Prupe.4G266300_v2.0.a1

Glycosyl transferases group 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0016637
Physical Location & Seq
Forward (+)
19894136 .. 19895644
1509 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G266300.1

Sequence Viewer

Length: 1509 bp
ATGGATTCAAATGAAACCCAACAACCCATTGAAAACCAACAACAACCCAATCCAACTCTCCCCTTCACATCAAAATTGAAACCCTATGCATTCTACTTATCATCCATCCTAATTCTCATCCTTGCTATCTCATTCAGTTTGACCAAAACAAACTACCTCAAAACCCAGCAGCTGAAGTACACATTCTCATCACAGCCCGCCATTTTTCAAGCTCTGTTTGGCTTTCTTCAACCAAAACAAAAAACCAAGCAAACCCAGGTACCAAATCCCATTTCCAAGCCCCCCTACTGTGTTCTGTGGATGGCTCCTTTCCTTTCAGGTGGTGGGTACAGTTCAGAAAGCTGGTCATATATTTTAGCCCTTCATGAACACAGTAAAAACCCAAACTTTAGAATGGCTATTGAGCAACATGGTGATCTGGAGTCCCTAGAATTCTGGGGGGGCTTGCCTAAATATATGAAGAACTTGGCAGTTGAGCTTTACCACACACAATGTAGTATGAAGGAGACCATAGTCATATGCCACAGTGAGCCTGGTGCTTGGAACCCTCCATTGTTTGAAACACTCCCTTGCCCACCAACTGCTTACCAAAATTTCAAGTCAGTGATTGGCCGGACCATGTTTGAGACCGATAGGGTGAATCCGGAACATGTGAAGCGCTGTAACCAAATGGATTATGTTTGGGTTCCTACTGAGTTTCATGTGTCTACATTTGTACAAAGTGGGGTTGATAAATCTAAGGTTGTGAAAATTGTGCAGCCTATTGATGTGAAGTTCTTTGATCCACTTGAGTATGAGCCTTTAAATCTTGCCTCTATAGGGAAGTTTGTTATGGGCAAGACAACCCAGAATTCGAAAGTGAAGAAGAAATTTGTATTTATGAGTATATTTAAGTGGGAGTATAGGAAAGGATGGGATGTGTTGTTGAAATCTTATTTGGAAGAATTCAGTGAGGCAGACGGGGTTGCCTTGTATCTTCTAACAAATCCTTATCACTCTGATAGAGATTTTGGGAATAAGATAGTGGAGTTTGTGGAAAAATCTGGAATGCAGAAACCAGTGACTGGTTGGGCTCCAGTTTATGTGATTGATACTCACATAGCACAGATTGATTTGCCACGGGTATACAAGGCAGCTGATGCATTTGTTCTTCCATCTAGAGGGGAAGGGTGGGGAAGGCCTCTTGTTGAAGCTATGGCAATGTCATTGCCAGTGATTGCTACGAATTGGTCAGGGCCAACGGAGTATCTGACTGAGGAGAATAGCTATCGACTGCCGGTGGATAGAATGAGCGACATAATGGAAGGGCCTTTCAGAGGGCATCGCTGGGCAGAACCTTCTGTCAGTAAGCTTCGTGTGCTCATGAGGCATGTGCTGAATAATGTAGAGGAAGCCAAGGTGAAAGGGGAAAAGGCAAGGGAAGACATGATCACAAGGTTCTCTCCTGAGATCGTTGCAGATATCGTTACTAAACATGTGCATAATATACTTCAGAAGGTGGATCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_trans_1_4 Glycos_transf_1

Protein Analysis

503

Amino Acids

57.51

Weight (kDa)

8.07

Isoelectric Point (pI)

40.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016637)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G10630
fragaria_vesca FvH4_5g28400
malus_domestica MD03G1165900.v1.1
prunus_persica Prupe.4G266300_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0331951
rosa_laevigata RLG00000029701
rosa_multiflora Rmu_sc0008128.1_g000008
rosa_roxburghii Rroxscaffold_4G00318590
rosa_rugosa Rorug01G0097100
rosa_samantha Rh1AG121200 Rh1BG092800 Rh1CG116000 Rh1DG127100
rosa_wichuraiana Rw1G009890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 259
AccB1I GGYRCC 1 cut(s) 259
AccB7I CCANNNNNTGG 1 cut(s) 1064
AccI GTMKAC 2 cut(s) 707, 1125
AccIII TCCGGA 1 cut(s) 643
AciI CCGC 1 cut(s) 198
AclWI GGATC 1 cut(s) 776
AcoI YGGCCR 1 cut(s) 610
AcsI RAATTY 5 cut(s) 431, 592, 850, 869, 944
AcuI CTGAAG 2 cut(s) 194, 1475
AfaI GTAC 4 cut(s) 179, 261, 329, 717
AfeI AGCGCT 1 cut(s) 659
AfiI CCNNNNNNNGG 4 cut(s) 819, 1064, 1160, 1316
AflIII ACRYGT 2 cut(s) 649, 1474
AgsI TTSAA 9 cut(s) 9, 32, 79, 209, 230, 560, 598, 928, 1190
AjnI CCWGG 2 cut(s) 255, 532
AjuI GAANNNNNNNTTGG 4 cut(s) 256, 288, 920, 952
AluBI AGCT 8 cut(s) 172, 212, 342, 478, 1136, 1193, 1266, 1351
AluI AGCT 8 cut(s) 172, 212, 342, 478, 1136, 1193, 1266, 1351
Alw21I GWGCWC 1 cut(s) 1362
Alw26I GTCTC 2 cut(s) 500, 620
AlwI GGATC 1 cut(s) 776
AlwNI CAGNNNCTG 2 cut(s) 172, 1064
Aor13HI TCCGGA 1 cut(s) 643
Aor51HI AGCGCT 1 cut(s) 659
AoxI GGCC 4 cut(s) 610, 1178, 1235, 1307
ApeKI GCWGC 3 cut(s) 169, 757, 1133
ApoI RAATTY 5 cut(s) 431, 592, 850, 869, 944
Asp718I GGTACC 1 cut(s) 259
AspLEI GCGC 1 cut(s) 660
AspS9I GGNCC 3 cut(s) 615, 1235, 1307
AsuHPI GGTGA 3 cut(s) 425, 649, 1411
AsuII TTCGAA 1 cut(s) 854
AvaII GGWCC 1 cut(s) 615
BaeI ACNNNNGTAYC 2 cut(s) 243, 276
BanI GGYRCC 1 cut(s) 259
BanII GRGCYC 1 cut(s) 1075
BbsI GAAGAC 1 cut(s) 1428
Bbv12I GWGCWC 1 cut(s) 1362
BbvI GCAGC 3 cut(s) 181, 769, 1145
BccI CCATC 4 cut(s) 113, 295, 906, 1162
BciT130I CCWGG 2 cut(s) 257, 534
BclI TGATCA 1 cut(s) 1428
BcoDI GTCTC 2 cut(s) 500, 620
BfaI CTAG 2 cut(s) 428, 1158
BfmI CTRYAG 1 cut(s) 816
BfoI RGCGCY 1 cut(s) 661
BisI GCNGC 3 cut(s) 170, 758, 1134
BlsI GCNGC 3 cut(s) 171, 759, 1135
Bme1390I CCNGG 2 cut(s) 257, 534
Bme18I GGWCC 1 cut(s) 615
BmgT120I GGNCC 3 cut(s) 615, 1235, 1307
BmiI GGNNCC 5 cut(s) 261, 306, 545, 687, 1074
BmrFI CCNGG 2 cut(s) 257, 534
BmsI GCATC 2 cut(s) 1129, 1330
BoxI GACNNNNGTC 1 cut(s) 512
BpiI GAAGAC 1 cut(s) 1428
BpmI CTGGAG 2 cut(s) 440, 1059
Bpu14I TTCGAA 1 cut(s) 854
BpuEI CTTGAG 1 cut(s) 809
BsaI GGTCTC 2 cut(s) 500, 620
BsaJI CCNNGG 3 cut(s) 255, 1118, 1395
BsaWI WCCGGW 1 cut(s) 643
Bsc4I CCNNNNNNNGG 4 cut(s) 819, 1064, 1160, 1316
Bse118I RCCGGY 1 cut(s) 1276
Bse1I ACTGG 4 cut(s) 1058, 1069, 1076, 1211
Bse3DI GCAATG 2 cut(s) 1205, 1206
BseAI TCCGGA 1 cut(s) 643
BseBI CCWGG 2 cut(s) 257, 534
BseDI CCNNGG 3 cut(s) 255, 1118, 1395
BseGI GGATG 6 cut(s) 101, 105, 117, 306, 917, 922
BseLI CCNNNNNNNGG 4 cut(s) 819, 1064, 1160, 1316
BseMI GCAATG 2 cut(s) 1205, 1206
BseMII CTCAG 3 cut(s) 684, 1245, 1437
BseNI ACTGG 4 cut(s) 1058, 1069, 1076, 1211
BseRI GAGGAG 1 cut(s) 1271
BseXI GCAGC 3 cut(s) 181, 769, 1145
BseYI CCCAGC 2 cut(s) 165, 1326
BsgI GTGCAG 1 cut(s) 776
BshFI GGCC 4 cut(s) 612, 1180, 1237, 1309
BshNI GGYRCC 1 cut(s) 259
BsiHKAI GWGCWC 1 cut(s) 1362
BsiSI CCGG 3 cut(s) 613, 644, 1277
BslFI GGGAC 1 cut(s) 409
BslI CCNNNNNNNGG 4 cut(s) 819, 1064, 1160, 1316
BsmAI GTCTC 2 cut(s) 500, 620
BsmFI GGGAC 1 cut(s) 409
BsmI GAATGC 2 cut(s) 89, 1053
BsnI GGCC 4 cut(s) 612, 1180, 1237, 1309
Bso31I GGTCTC 2 cut(s) 500, 620
Bsp119I TTCGAA 1 cut(s) 854
Bsp1286I GDGCHC 2 cut(s) 1075, 1362
Bsp13I TCCGGA 1 cut(s) 643
Bsp1407I TGTACA 1 cut(s) 715
Bsp143I GATC 5 cut(s) 415, 781, 1428, 1449, 1501
BspACI CCGC 1 cut(s) 198
BspANI GGCC 4 cut(s) 612, 1180, 1237, 1309
BspCNI CTCAG 3 cut(s) 685, 1246, 1438
BspEI TCCGGA 1 cut(s) 643
BspHI TCATGA 2 cut(s) 364, 1362
BspLI GGNNCC 5 cut(s) 261, 306, 545, 687, 1074
BspPI GGATC 1 cut(s) 776
BspT104I TTCGAA 1 cut(s) 854
BspT107I GGYRCC 1 cut(s) 259
BspTNI GGTCTC 2 cut(s) 500, 620
BsrDI GCAATG 2 cut(s) 1205, 1206
BsrFI RCCGGY 1 cut(s) 1276
BsrGI TGTACA 1 cut(s) 715
BsrI ACTGG 4 cut(s) 1058, 1069, 1076, 1211
BssAI RCCGGY 1 cut(s) 1276
BssECI CCNNGG 3 cut(s) 255, 1118, 1395
BssMI GATC 5 cut(s) 415, 781, 1428, 1449, 1501
BssNAI GTATAC 1 cut(s) 1126
BssT1I CCWWGG 1 cut(s) 1395
Bst1107I GTATAC 1 cut(s) 1126
Bst2UI CCWGG 2 cut(s) 257, 534
Bst4CI ACNGT 4 cut(s) 290, 332, 374, 527
BstAPI GCANNNNNTGC 1 cut(s) 1139
BstAUI TGTACA 1 cut(s) 715
BstBI TTCGAA 1 cut(s) 854
BstC8I GCNNGC 2 cut(s) 198, 446
BstDEI CTNAG 4 cut(s) 693, 738, 1254, 1446
BstDSI CCRYGG 1 cut(s) 1118
BstENI CCTNNNNNAGG 1 cut(s) 1314
BstF5I GGATG 6 cut(s) 101, 105, 117, 306, 917, 922
BstH2I RGCGCY 1 cut(s) 661
BstHHI GCGC 1 cut(s) 660
BstKTI GATC 5 cut(s) 418, 784, 1431, 1452, 1504
BstMAI GTCTC 2 cut(s) 500, 620
BstMBI GATC 5 cut(s) 415, 781, 1428, 1449, 1501
BstMWI GCNNNNNNNGC 3 cut(s) 1139, 1357, 1366
BstNI CCWGG 2 cut(s) 257, 534
BstNSI RCATGY 3 cut(s) 653, 1373, 1478
BstPAI GACNNNNGTC 1 cut(s) 512
BstSCI CCNGG 2 cut(s) 255, 532
BstSFI CTRYAG 1 cut(s) 816
BstV1I GCAGC 3 cut(s) 181, 769, 1145
BstV2I GAAGAC 1 cut(s) 1428
BstZ17I GTATAC 1 cut(s) 1126
BsuRI GGCC 4 cut(s) 612, 1180, 1237, 1309
BtgI CCRYGG 1 cut(s) 1118
BtgZI GCGATG 1 cut(s) 1307
BtsCI GGATG 6 cut(s) 101, 105, 117, 306, 917, 922
BtsIMutI CAGTG 5 cut(s) 532, 609, 955, 1065, 1218
Cac8I GCNNGC 2 cut(s) 198, 446
CaiI CAGNNNCTG 2 cut(s) 172, 1064
CciI TCATGA 2 cut(s) 364, 1362
CfoI GCGC 1 cut(s) 660
Cfr10I RCCGGY 1 cut(s) 1276
Cfr13I GGNCC 3 cut(s) 615, 1235, 1307
Csp6I GTAC 4 cut(s) 178, 260, 328, 716
CviAII CATG 9 cut(s) 365, 410, 619, 650, 701, 1363, 1370, 1426, 1475
CviQI GTAC 4 cut(s) 178, 260, 328, 716
DdeI CTNAG 4 cut(s) 693, 738, 1254, 1446
DpnI GATC 5 cut(s) 417, 783, 1430, 1451, 1503
DpnII GATC 5 cut(s) 415, 781, 1428, 1449, 1501
DraI TTTAAA 1 cut(s) 804
EaeI YGGCCR 1 cut(s) 610
Eco130I CCWWGG 1 cut(s) 1395
Eco147I AGGCCT 1 cut(s) 1180
Eco24I GRGCYC 1 cut(s) 1075
Eco31I GGTCTC 2 cut(s) 500, 620
Eco32I GATATC 1 cut(s) 1462
Eco47I GGWCC 1 cut(s) 615
Eco47III AGCGCT 1 cut(s) 659
Eco57I CTGAAG 2 cut(s) 194, 1475
EcoNI CCTNNNNNAGG 1 cut(s) 1314
EcoO109I RGGNCCY 1 cut(s) 1307
EcoRI GAATTC 3 cut(s) 431, 850, 944
EcoRII CCWGG 2 cut(s) 255, 532
EcoRV GATATC 1 cut(s) 1462
EcoT14I CCWWGG 1 cut(s) 1395
EcoT22I ATGCAT 2 cut(s) 91, 1144
EcoT38I GRGCYC 1 cut(s) 1075
ErhI CCWWGG 1 cut(s) 1395
FaeI CATG 9 cut(s) 368, 413, 622, 653, 704, 1366, 1373, 1429, 1478
FaqI GGGAC 1 cut(s) 409
FatI CATG 9 cut(s) 364, 409, 618, 649, 700, 1362, 1369, 1425, 1474
FauI CCCGC 1 cut(s) 205
FauNDI CATATG 1 cut(s) 518
FbaI TGATCA 1 cut(s) 1428
FblI GTMKAC 2 cut(s) 707, 1125
Fnu4HI GCNGC 3 cut(s) 170, 758, 1134
FokI GGATG 6 cut(s) 88, 92, 104, 313, 924, 929
FriOI GRGCYC 1 cut(s) 1075
Fsp4HI GCNGC 3 cut(s) 170, 758, 1134
FspBI CTAG 2 cut(s) 428, 1158
GlaI GCGC 1 cut(s) 659
GluI GCNGC 3 cut(s) 170, 758, 1134
GsaI CCCAGC 2 cut(s) 169, 1330
GsuI CTGGAG 2 cut(s) 440, 1059
HaeII RGCGCY 1 cut(s) 661
HaeIII GGCC 4 cut(s) 612, 1180, 1237, 1309
HapII CCGG 3 cut(s) 613, 644, 1277
HhaI GCGC 1 cut(s) 660
Hin1II CATG 9 cut(s) 368, 413, 622, 653, 704, 1366, 1373, 1429, 1478
Hin6I GCGC 1 cut(s) 658
HinP1I GCGC 1 cut(s) 658
HindIII AAGCTT 1 cut(s) 1349
HinfI GANTC 3 cut(s) 5, 422, 640
HpaII CCGG 3 cut(s) 613, 644, 1277
HphI GGTGA 3 cut(s) 425, 649, 1411
Hpy166II GTNNAC 3 cut(s) 180, 708, 1126
Hpy188I TCNGA 5 cut(s) 337, 1000, 1251, 1316, 1494
Hpy188III TCNNGA 7 cut(s) 365, 419, 644, 1044, 1158, 1363, 1445
Hpy8I GTNNAC 3 cut(s) 180, 708, 1126
HpyAV CCTTC 8 cut(s) 73, 371, 496, 1160, 1170, 1298, 1347, 1489
HpyCH4III ACNGT 4 cut(s) 290, 332, 374, 527
HpyCH4V TGCA 6 cut(s) 89, 757, 1051, 1142, 1457, 1480
HpyF10VI GCNNNNNNNGC 3 cut(s) 1139, 1357, 1366
HpyF3I CTNAG 4 cut(s) 693, 738, 1254, 1446
Hsp92II CATG 9 cut(s) 368, 413, 622, 653, 704, 1366, 1373, 1429, 1478
HspAI GCGC 1 cut(s) 658
Kpn2I TCCGGA 1 cut(s) 643
KpnI GGTACC 1 cut(s) 263
Ksp22I TGATCA 1 cut(s) 1428
Kzo9I GATC 5 cut(s) 415, 781, 1428, 1449, 1501
LmnI GCTCC 2 cut(s) 310, 1078
Lsp1109I GCAGC 3 cut(s) 181, 769, 1145
LweI GCATC 2 cut(s) 1129, 1330
MaeI CTAG 2 cut(s) 428, 1158
MaeIII GTNAC 3 cut(s) 662, 1060, 1465
MalI GATC 5 cut(s) 417, 783, 1430, 1451, 1503
MboI GATC 5 cut(s) 415, 781, 1428, 1449, 1501
MboII GAAGA 8 cut(s) 218, 472, 874, 877, 953, 968, 1142, 1433
MhlI GDGCHC 2 cut(s) 1075, 1362
MluCI AATT 9 cut(s) 74, 111, 431, 592, 750, 850, 869, 944, 1225
MlyI GAGTC 1 cut(s) 431
MmeI TCCRAC 1 cut(s) 77
Mph1103I ATGCAT 2 cut(s) 91, 1144
MroI TCCGGA 1 cut(s) 643
MseI TTAA 2 cut(s) 803, 891
MspA1I CMGCKG 2 cut(s) 172, 1136
MspI CCGG 3 cut(s) 613, 644, 1277
MspR9I CCNGG 2 cut(s) 257, 534
Mva1269I GAATGC 2 cut(s) 89, 1053
MvaI CCWGG 2 cut(s) 257, 534
MwoI GCNNNNNNNGC 3 cut(s) 1139, 1357, 1366
NdeI CATATG 1 cut(s) 518
NdeII GATC 5 cut(s) 415, 781, 1428, 1449, 1501
NlaIII CATG 9 cut(s) 368, 413, 622, 653, 704, 1366, 1373, 1429, 1478
NlaIV GGNNCC 5 cut(s) 261, 306, 545, 687, 1074
NmuCI GTSAC 1 cut(s) 1060
NsiI ATGCAT 2 cut(s) 91, 1144
NspI RCATGY 3 cut(s) 653, 1373, 1478
NspV TTCGAA 1 cut(s) 854
PagI TCATGA 2 cut(s) 364, 1362
PceI AGGCCT 1 cut(s) 1180
PciI ACATGT 2 cut(s) 649, 1474
PctI GAATGC 2 cut(s) 89, 1053
PfeI GAWTC 2 cut(s) 5, 640
PflMI CCANNNNNTGG 1 cut(s) 1064
PkrI GCNGC 3 cut(s) 171, 759, 1135
PleI GAGTC 1 cut(s) 430
PpsI GAGTC 1 cut(s) 430
PscI ACATGT 2 cut(s) 649, 1474
PshAI GACNNNNGTC 1 cut(s) 512
Psp6I CCWGG 2 cut(s) 255, 532
PspFI CCCAGC 2 cut(s) 165, 1326
PspGI CCWGG 2 cut(s) 255, 532
PspN4I GGNNCC 5 cut(s) 261, 306, 545, 687, 1074
PspPI GGNCC 3 cut(s) 615, 1235, 1307
PstNI CAGNNNCTG 2 cut(s) 172, 1064
PvuII CAGCTG 2 cut(s) 172, 1136
RsaI GTAC 4 cut(s) 179, 261, 329, 717
RsaNI GTAC 4 cut(s) 178, 260, 328, 716
SaqAI TTAA 2 cut(s) 803, 891
SatI GCNGC 3 cut(s) 170, 758, 1134
Sau3AI GATC 5 cut(s) 415, 781, 1428, 1449, 1501
Sau96I GGNCC 3 cut(s) 615, 1235, 1307
SchI GAGTC 1 cut(s) 431
ScrFI CCNGG 2 cut(s) 257, 534
SduI GDGCHC 2 cut(s) 1075, 1362
SfaNI GCATC 2 cut(s) 1129, 1330
SfcI CTRYAG 1 cut(s) 816
SfuI TTCGAA 1 cut(s) 854
SinI GGWCC 1 cut(s) 615
SmlI CTYRAG 1 cut(s) 788
SmoI CTYRAG 1 cut(s) 788
Sse9I AATT 9 cut(s) 74, 111, 431, 592, 750, 850, 869, 944, 1225
SseBI AGGCCT 1 cut(s) 1180
SsiI CCGC 1 cut(s) 198
SspMI CTAG 2 cut(s) 428, 1158
StuI AGGCCT 1 cut(s) 1180
StyD4I CCNGG 2 cut(s) 255, 532
StyI CCWWGG 1 cut(s) 1395
TaaI ACNGT 4 cut(s) 290, 332, 374, 527
TaqI TCGA 2 cut(s) 854, 1270
TaqII GACCGA 1 cut(s) 644
TasI AATT 9 cut(s) 74, 111, 431, 592, 750, 850, 869, 944, 1225
TatI WGTACW 2 cut(s) 177, 715
TfiI GAWTC 2 cut(s) 5, 640
Tru1I TTAA 2 cut(s) 803, 891
Tru9I TTAA 2 cut(s) 803, 891
TscAI CASTG 5 cut(s) 532, 609, 955, 1065, 1218
TseFI GTSAC 1 cut(s) 1060
TseI GCWGC 3 cut(s) 169, 757, 1133
Tsp45I GTSAC 1 cut(s) 1060
TspDTI ATGAA 6 cut(s) 27, 353, 381, 473, 515, 689
TspGWI ACGGA 1 cut(s) 1256
TspRI CASTG 5 cut(s) 532, 609, 955, 1065, 1218
Van91I CCANNNNNTGG 1 cut(s) 1064
VpaK11BI GGWCC 1 cut(s) 615
XagI CCTNNNNNAGG 1 cut(s) 1314
XapI RAATTY 5 cut(s) 431, 592, 850, 869, 944
XbaI TCTAGA 1 cut(s) 1157
XceI RCATGY 3 cut(s) 653, 1373, 1478
XcmI CCANNNNNNNNNTGG 2 cut(s) 530, 1065
XmiI GTMKAC 2 cut(s) 707, 1125
XspI CTAG 2 cut(s) 428, 1158
Zsp2I ATGCAT 2 cut(s) 91, 1144
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.