Prupe.5G147700_v2.0.a1

recQ-mediated genome instability protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0015275
Physical Location & Seq
Forward (+)
13537130 .. 13541811
4682 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G147700.1

Sequence Viewer

Length: 2106 bp
ATGCTCAGAAGACGTCTCAGGCTCAGCTACTCTTCCGACGAAGAAGAACAACCACAACCACAACAACACCAGAATGAAGAACCTCAGCAGCAATCGTCAGCTGTCGCTCACCCACCCGTTACCCTAGCCCCTCCAAACCCTTACCCCTCCGAACCGCTCCCAATCACCGACGACGACGACGAGTTCATCGATGTCTCCGACAACCTCTCGTCTCCGTCACCTCAGCCGTCTCCGGAACCCGAGAGCGACAATCGACCACATCATCCTCCGCCGGCTCAGGATCCGGCACCGGAACCCATTAACCGGCCGCCTCCACCTCCATCTCCATCTCCACCGAGCTCCGGCGGGTGTCCGATAAGCGAACATCTCCAGGGGTTAGGGTTAAGGCTAAAGAGGGAGTGGCTTGATGCTTCTGTCCATGGACTCCAGCAATCCGTAACGGGATTTCAAAATTTTGATGTGGAGACGAAGGCGAAGCTCTGCTTGGAGCAGTTTTTGGTTTCAGATATGAATCTGTCCGGCGGAGGCGTGCTTCCAGAAAATGTGGCTTCTCTCCATCTCGTCGATCTTCCCGGACCTTATGTGTTGCAGGTTGATGAGATTGTTAATATCAGCAATCCTCTCAAGATTAGGTATCAGAAAGCCGCCGTTGGACTGAAGAGGTGTCTCAAGTTGTCTATAACAGACGGTGTTCAACGCGTCTTTGCCATGGAATATAGGCCTATTCAGGCGCTTGAAGCTTTGGCTCCTGCTGGTTTGAAGGTTGCTCTGTGTAATGTGCATATACGCCACGGACTTCTTATGCTGGTCCCTGAATCTGTTGAAGTTCTAGGAGGATTAGTTGAGGAACTGGATGCTGCACGTCAACGCCTTGTTGATGAAGTAAATAAGCCACCTAGAGGAAACAGAACCAGGAATGGAGTGGTTCCTCCTCTAGCTACAAGGTTAACTCTTGCTGCATGGCCTCCTAACGGTGTTCATGATGCTGGACACACCAATAGTTCAACAATGGAAGCCCCAACCCCATTTCAGGCGTCTAACCAAGGTGCTACATTCTCTGTATCAGCCAGAAATACCAGCTCCGTATCTTCTAGAAACACCAGCCCAGTGACTGCCGAAGAATCTACTGTTCCTCTCAGTGGAGAACACTCTGTTGTTAACCCATCATCTACTGATGTATTTGGTGTTGAAGAGATGCCTATGGATACTACTCCCTATAGCAGAAGAAATGCTATTCCCAACCCATTATCTGATGATTTTGGTGTTGAGAATCTCCACATAAATAGTGCTGGTCATAGCAGGGAAAGTCGAGTACCTGAAAATGTCATTCCTAATCCATCCTTTGAGGATGCTTTGGATGTTGAAGATGTCGAAATGGATGCCATTCATATTGGGGGAGAAAACCATACCTCAGATTCTATTTTGAATGATGAAGATATCAATATGATTAACGAGGGCGAAACTCATGGGGTTTACCCATCGTCTGATGCTATTTTGAATGATGAGGATATCAATGTGGTTAATGAGGTCGAGCGCCAAGAAATACTATCTGGAGACCACGAACCACCTTTCAGTTACCTATGTAGTTTGTCAGCCAAGTTAGCTATGATGAAGAACACACATTCAATCCAGGGGAAGGTTAAGTGTGTTTTGACTGGTGTGAAGAAGTTCCAATACACACATACATATGAGCTTCAGGCGTATGTTGATGACGGCACTCTCATTAAAGAAATTCTCGTTGATCACAATGTTGTACAGGAGGGGATAGGTCATTCTCCTGAGGAGGTCAAAGCTGCCATTTCTTCTCCAGACAAAACAATAGTCCGTAACATGAAGGAGAAAATGAAGCAGTTTCAAAAATTCTTAACAAATTTTGAGGGCATAATGCTTATTGAGATGAGATTGCATGATCTTCCAATTGCCCTTGAGATGAAGCAGGGTTGTACTCAATCTGATGCGTGGTTGCTTTTGAGGAGAATAAAGGCCTCCCCTCCAGCTCGGGCATCAGGGCATGCCTCCTCTGCAGCTCGGACATCACAGTATCCTTCCTCTCCAGCTCGGACTTCACAACATCTCTCCTCCCACCCCATTGATATATCCCCCTGA

Protein Analysis

702

Amino Acids

76.63

Weight (kDa)

5.1

Isoelectric Point (pI)

60.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015275)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G63540 AT5G63540
fragaria_vesca FvH4_1g26610
malus_domestica MD14G1152700.v1.1
prunus_persica Prupe.5G147700_v2.0.a1
pyrus_communis pycom14g12710
rosa_chinensis RchiOBHm_Chr7g0189541
rosa_laevigata RLG00000004602
rosa_multiflora Rmu_sc0001924.1_g000011
rosa_roxburghii Rroxscaffold_3G00265320
rosa_rugosa Rorug06G0498900
rosa_samantha Rh7AG103800 Rh7BG106500 Rh7CG108600 Rh7DG105900
rosa_wichuraiana Rw7G008960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 16
Acc36I ACCTGC 1 cut(s) 580
AccB1I GGYRCC 1 cut(s) 286
AccBSI CCGCTC 1 cut(s) 157
AccII CGCG 1 cut(s) 699
AccIII TCCGGA 1 cut(s) 232
AciI CCGC 6 cut(s) 155, 269, 308, 345, 522, 645
AclWI GGATC 2 cut(s) 275, 288
AcoI YGGCCR 1 cut(s) 305
AcsI RAATTY 4 cut(s) 451, 1731, 1859, 1870
AcuI CTGAAG 2 cut(s) 677, 1679
AcyI GRCGYC 2 cut(s) 13, 1034
AfaI GTAC 3 cut(s) 1314, 1755, 1945
AfiI CCNNNNNNNGG 7 cut(s) 303, 341, 899, 971, 1030, 1139, 1636
AflIII ACRYGT 1 cut(s) 697
AjiI CACGTC 1 cut(s) 863
AjnI CCWGG 3 cut(s) 369, 911, 1629
AjuI GAANNNNNNNTTGG 2 cut(s) 467, 499
Alw21I GWGCWC 1 cut(s) 341
Alw26I GTCTC 7 cut(s) 20, 199, 216, 234, 458, 671, 1548
AlwI GGATC 2 cut(s) 275, 288
AlwNI CAGNNNCTG 1 cut(s) 1112
Ama87I CYCGRG 2 cut(s) 239, 1998
Aor13HI TCCGGA 1 cut(s) 232
AoxI GGCC 4 cut(s) 305, 719, 962, 1983
ApeKI GCWGC 5 cut(s) 88, 857, 956, 1793, 2024
ApoI RAATTY 4 cut(s) 451, 1731, 1859, 1870
ArsI GACNNNNNNTTYG 2 cut(s) 1806, 1838
Asp700I GAANNNNTTC 1 cut(s) 1667
AspLEI GCGC 2 cut(s) 733, 1536
AspS9I GGNCC 2 cut(s) 575, 808
AsuC2I CCSGG 1 cut(s) 573
AsuHPI GGTGA 3 cut(s) 101, 157, 210
AvaI CYCGRG 2 cut(s) 239, 1998
AvaII GGWCC 2 cut(s) 575, 808
AxyI CCTNAGG 1 cut(s) 1779
BamHI GGATCC 1 cut(s) 280
BanI GGYRCC 1 cut(s) 286
BanII GRGCYC 1 cut(s) 341
BarI GAAGNNNNNNTAC 2 cut(s) 1658, 1690
BbsI GAAGAC 1 cut(s) 16
Bbv12I GWGCWC 1 cut(s) 341
BbvCI CCTCAGC 2 cut(s) 84, 222
BbvI GCAGC 5 cut(s) 100, 844, 943, 1780, 2036
BccI CCATC 6 cut(s) 328, 334, 564, 1171, 1345, 1486
BceAI ACGGC 3 cut(s) 211, 632, 1729
BcgI CGANNNNNNTGC 2 cut(s) 1361, 1395
BciT130I CCWGG 3 cut(s) 371, 913, 1631
BciVI GTATCC 2 cut(s) 1198, 2052
BclI TGATCA 1 cut(s) 1741
BcnI CCSGG 1 cut(s) 573
BcoDI GTCTC 7 cut(s) 20, 199, 216, 234, 458, 671, 1548
BfaI CTAG 5 cut(s) 125, 830, 897, 935, 1092
BfmI CTRYAG 2 cut(s) 1216, 2022
BfoI RGCGCY 2 cut(s) 734, 1537
BfuAI ACCTGC 1 cut(s) 580
BfuI GTATCC 2 cut(s) 1198, 2052
BisI GCNGC 7 cut(s) 89, 308, 645, 858, 957, 1794, 2025
BlpI GCTNAGC 1 cut(s) 23
BlsI GCNGC 7 cut(s) 90, 309, 646, 859, 958, 1795, 2026
Bme1390I CCNGG 4 cut(s) 371, 573, 913, 1631
Bme18I GGWCC 2 cut(s) 575, 808
BmeT110I CYCGRG 2 cut(s) 239, 1998
BmgBI CACGTC 1 cut(s) 863
BmgT120I GGNCC 2 cut(s) 575, 808
BmiI GGNNCC 7 cut(s) 237, 282, 288, 294, 747, 810, 927
BmrFI CCNGG 4 cut(s) 371, 573, 913, 1631
BmrI ACTGGG 1 cut(s) 1100
BmsI GCATC 9 cut(s) 397, 844, 973, 1185, 1339, 1369, 1477, 1945, 2012
BmuI ACTGGG 1 cut(s) 1100
BpiI GAAGAC 1 cut(s) 16
BpmI CTGGAG 6 cut(s) 353, 410, 1572, 1791, 1977, 2037
Bpu10I CCTNAGC 3 cut(s) 84, 222, 276
Bpu1102I GCTNAGC 1 cut(s) 23
BpuEI CTTGAG 3 cut(s) 608, 653, 1946
BpuMI CCSGG 1 cut(s) 573
Bsa29I ATCGAT 1 cut(s) 189
BsaBI GATNNNNATC 1 cut(s) 510
BsaHI GRCGYC 2 cut(s) 13, 1034
BsaI GGTCTC 1 cut(s) 1548
BsaJI CCNNGG 6 cut(s) 370, 418, 708, 790, 1042, 1630
BsaWI WCCGGW 2 cut(s) 232, 289
BsaXI ACNNNNNCTCC 2 cut(s) 825, 855
Bsc4I CCNNNNNNNGG 7 cut(s) 303, 341, 899, 971, 1030, 1139, 1636
Bse118I RCCGGY 2 cut(s) 271, 303
Bse1I ACTGG 3 cut(s) 855, 1106, 1660
Bse21I CCTNAGG 1 cut(s) 1779
Bse8I GATNNNNATC 1 cut(s) 510
BseAI TCCGGA 1 cut(s) 232
BseBI CCWGG 3 cut(s) 371, 913, 1631
BseCI ATCGAT 1 cut(s) 189
BseDI CCNNGG 6 cut(s) 370, 418, 708, 790, 1042, 1630
BseGI GGATG 6 cut(s) 262, 859, 1337, 1354, 1363, 1384
BseJI GATNNNNATC 1 cut(s) 510
BseLI CCNNNNNNNGG 7 cut(s) 303, 341, 899, 971, 1030, 1139, 1636
BseMII CTCAG 9 cut(s) 19, 31, 37, 98, 236, 290, 1150, 1425, 1770
BseNI ACTGG 3 cut(s) 855, 1106, 1660
BseRI GAGGAG 5 cut(s) 921, 1796, 1987, 2008, 2068
BseX3I CGGCCG 1 cut(s) 305
BseXI GCAGC 5 cut(s) 100, 844, 943, 1780, 2036
BsgI GTGCAG 1 cut(s) 843
Bsh1236I CGCG 1 cut(s) 699
Bsh1285I CGRYCG 1 cut(s) 308
BshFI GGCC 4 cut(s) 307, 721, 964, 1985
BshNI GGYRCC 1 cut(s) 286
BshVI ATCGAT 1 cut(s) 189
BsiEI CGRYCG 1 cut(s) 308
BsiHKAI GWGCWC 1 cut(s) 341
BsiHKCI CYCGRG 2 cut(s) 239, 1998
BsiSI CCGG 8 cut(s) 233, 272, 284, 290, 304, 342, 519, 573
BslFI GGGAC 1 cut(s) 794
BslI CCNNNNNNNGG 7 cut(s) 303, 341, 899, 971, 1030, 1139, 1636
BsmAI GTCTC 7 cut(s) 20, 199, 216, 234, 458, 671, 1548
BsmBI CGTCTC 4 cut(s) 20, 216, 234, 458
BsmFI GGGAC 1 cut(s) 794
BsnI GGCC 4 cut(s) 307, 721, 964, 1985
Bso31I GGTCTC 1 cut(s) 1548
BsoBI CYCGRG 2 cut(s) 239, 1998
Bsp1286I GDGCHC 1 cut(s) 341
Bsp13I TCCGGA 1 cut(s) 232
Bsp1407I TGTACA 1 cut(s) 1753
Bsp143I GATC 4 cut(s) 280, 565, 1741, 1909
Bsp1720I GCTNAGC 1 cut(s) 23
Bsp19I CCATGG 2 cut(s) 418, 708
BspACI CCGC 6 cut(s) 155, 269, 308, 345, 522, 645
BspANI GGCC 4 cut(s) 307, 721, 964, 1985
BspCNI CTCAG 9 cut(s) 18, 30, 36, 97, 235, 289, 1149, 1424, 1771
BspDI ATCGAT 1 cut(s) 189
BspEI TCCGGA 1 cut(s) 232
BspFNI CGCG 1 cut(s) 699
BspHI TCATGA 1 cut(s) 979
BspLI GGNNCC 7 cut(s) 237, 282, 288, 294, 747, 810, 927
BspMAI CTGCAG 1 cut(s) 2026
BspMI ACCTGC 1 cut(s) 580
BspPI GGATC 2 cut(s) 275, 288
BspT107I GGYRCC 1 cut(s) 286
BspTNI GGTCTC 1 cut(s) 1548
BsrBI CCGCTC 1 cut(s) 157
BsrFI RCCGGY 2 cut(s) 271, 303
BsrGI TGTACA 1 cut(s) 1753
BsrI ACTGG 3 cut(s) 855, 1106, 1660
BssAI RCCGGY 2 cut(s) 271, 303
BssECI CCNNGG 6 cut(s) 370, 418, 708, 790, 1042, 1630
BssMI GATC 4 cut(s) 280, 565, 1741, 1909
BssNI GRCGYC 2 cut(s) 13, 1034
BssT1I CCWWGG 3 cut(s) 418, 708, 1042
Bst2UI CCWGG 3 cut(s) 371, 913, 1631
Bst4CI ACNGT 4 cut(s) 689, 974, 1129, 2040
Bst6I CTCTTC 3 cut(s) 37, 653, 1185
BstACI GRCGYC 2 cut(s) 13, 1034
BstAUI TGTACA 1 cut(s) 1753
BstC8I GCNNGC 3 cut(s) 273, 530, 2013
BstDEI CTNAG 9 cut(s) 5, 17, 23, 84, 222, 276, 1136, 1411, 1779
BstDSI CCRYGG 3 cut(s) 418, 708, 790
BstF5I GGATG 6 cut(s) 262, 859, 1337, 1354, 1363, 1384
BstFNI CGCG 1 cut(s) 699
BstH2I RGCGCY 2 cut(s) 734, 1537
BstHHI GCGC 2 cut(s) 733, 1536
BstKTI GATC 4 cut(s) 283, 568, 1744, 1912
BstMAI GTCTC 7 cut(s) 20, 199, 216, 234, 458, 671, 1548
BstMBI GATC 4 cut(s) 280, 565, 1741, 1909
BstMCI CGRYCG 1 cut(s) 308
BstMWI GCNNNNNNNGC 3 cut(s) 737, 1601, 2021
BstNI CCWGG 3 cut(s) 371, 913, 1631
BstNSI RCATGY 1 cut(s) 2015
BstSCI CCNGG 4 cut(s) 369, 571, 911, 1629
BstSFI CTRYAG 2 cut(s) 1216, 2022
BstUI CGCG 1 cut(s) 699
BstV1I GCAGC 5 cut(s) 100, 844, 943, 1780, 2036
BstV2I GAAGAC 1 cut(s) 16
BstX2I RGATCY 1 cut(s) 280
BstYI RGATCY 1 cut(s) 280
BstZI CGGCCG 1 cut(s) 305
Bsu15I ATCGAT 1 cut(s) 189
Bsu36I CCTNAGG 1 cut(s) 1779
BsuI GTATCC 2 cut(s) 1198, 2052
BsuRI GGCC 4 cut(s) 307, 721, 964, 1985
BsuTUI ATCGAT 1 cut(s) 189
BtgI CCRYGG 3 cut(s) 418, 708, 790
BtrI CACGTC 1 cut(s) 863
BtsCI GGATG 6 cut(s) 262, 859, 1337, 1354, 1363, 1384
BtsIMutI CAGTG 2 cut(s) 1113, 1144
BveI ACCTGC 1 cut(s) 580
Cac8I GCNNGC 3 cut(s) 273, 530, 2013
CaiI CAGNNNCTG 1 cut(s) 1112
CciI TCATGA 1 cut(s) 979
CfoI GCGC 2 cut(s) 733, 1536
Cfr10I RCCGGY 2 cut(s) 271, 303
Cfr13I GGNCC 2 cut(s) 575, 808
ClaI ATCGAT 1 cut(s) 189
CseI GACGC 2 cut(s) 688, 1023
Csp6I GTAC 3 cut(s) 1313, 1754, 1944
CviAII CATG 8 cut(s) 419, 709, 960, 980, 1466, 1831, 1907, 2012
CviQI GTAC 3 cut(s) 1313, 1754, 1944
DdeI CTNAG 9 cut(s) 5, 17, 23, 84, 222, 276, 1136, 1411, 1779
DpnI GATC 4 cut(s) 282, 567, 1743, 1911
DpnII GATC 4 cut(s) 280, 565, 1741, 1909
EaeI YGGCCR 1 cut(s) 305
EagI CGGCCG 1 cut(s) 305
Eam1104I CTCTTC 3 cut(s) 37, 653, 1185
EarI CTCTTC 3 cut(s) 37, 653, 1185
EciI GGCGGA 2 cut(s) 258, 537
Ecl136II GAGCTC 1 cut(s) 339
EclXI CGGCCG 1 cut(s) 305
Eco130I CCWWGG 3 cut(s) 418, 708, 1042
Eco147I AGGCCT 2 cut(s) 721, 1985
Eco24I GRGCYC 1 cut(s) 341
Eco31I GGTCTC 1 cut(s) 1548
Eco32I GATATC 2 cut(s) 1438, 1510
Eco47I GGWCC 2 cut(s) 575, 808
Eco52I CGGCCG 1 cut(s) 305
Eco53kI GAGCTC 1 cut(s) 339
Eco57I CTGAAG 2 cut(s) 677, 1679
Eco81I CCTNAGG 1 cut(s) 1779
Eco88I CYCGRG 2 cut(s) 239, 1998
EcoICRI GAGCTC 1 cut(s) 339
EcoRII CCWGG 3 cut(s) 369, 911, 1629
EcoRV GATATC 2 cut(s) 1438, 1510
EcoT14I CCWWGG 3 cut(s) 418, 708, 1042
EcoT38I GRGCYC 1 cut(s) 341
ErhI CCWWGG 3 cut(s) 418, 708, 1042
Esp3I CGTCTC 4 cut(s) 20, 216, 234, 458
FaeI CATG 8 cut(s) 422, 712, 963, 983, 1469, 1834, 1910, 2015
FalI AAGNNNNNCTT 2 cut(s) 467, 499
FaqI GGGAC 1 cut(s) 794
FatI CATG 8 cut(s) 418, 708, 959, 979, 1465, 1830, 1906, 2011
FauI CCCGC 1 cut(s) 338
FauNDI CATATG 1 cut(s) 1687
FbaI TGATCA 1 cut(s) 1741
Fnu4HI GCNGC 7 cut(s) 89, 308, 645, 858, 957, 1794, 2025
FokI GGATG 6 cut(s) 249, 866, 1324, 1361, 1370, 1391
FriOI GRGCYC 1 cut(s) 341
Fsp4HI GCNGC 7 cut(s) 89, 308, 645, 858, 957, 1794, 2025
FspBI CTAG 5 cut(s) 125, 830, 897, 935, 1092
GlaI GCGC 2 cut(s) 732, 1535
GluI GCNGC 7 cut(s) 89, 308, 645, 858, 957, 1794, 2025
GsuI CTGGAG 6 cut(s) 353, 410, 1572, 1791, 1977, 2037
HaeII RGCGCY 2 cut(s) 734, 1537
HaeIII GGCC 4 cut(s) 307, 721, 964, 1985
HapII CCGG 8 cut(s) 233, 272, 284, 290, 304, 342, 519, 573
HgaI GACGC 2 cut(s) 688, 1023
HhaI GCGC 2 cut(s) 733, 1536
Hin1I GRCGYC 2 cut(s) 13, 1034
Hin1II CATG 8 cut(s) 422, 712, 963, 983, 1469, 1834, 1910, 2015
Hin6I GCGC 2 cut(s) 731, 1534
HinP1I GCGC 2 cut(s) 731, 1534
HincII GTYRAC 3 cut(s) 866, 948, 1159
HindII GTYRAC 3 cut(s) 866, 948, 1159
HindIII AAGCTT 1 cut(s) 738
HinfI GANTC 6 cut(s) 423, 511, 815, 1121, 1270, 1415
HpaI GTTAAC 2 cut(s) 948, 1159
HpaII CCGG 8 cut(s) 233, 272, 284, 290, 304, 342, 519, 573
HphI GGTGA 3 cut(s) 101, 157, 210
Hpy166II GTNNAC 4 cut(s) 866, 948, 1159, 1474
Hpy188III TCNNGA 9 cut(s) 233, 278, 536, 625, 980, 1092, 1551, 1778, 1808
Hpy8I GTNNAC 4 cut(s) 866, 948, 1159, 1474
Hpy99I CGWCG 6 cut(s) 41, 173, 176, 179, 182, 566
HpyAV CCTTC 5 cut(s) 463, 754, 1630, 1828, 2055
HpyCH4III ACNGT 4 cut(s) 689, 974, 1129, 2040
HpyCH4IV ACGT 2 cut(s) 13, 862
HpyCH4V TGCA 6 cut(s) 589, 781, 860, 959, 1906, 2024
HpyF10VI GCNNNNNNNGC 3 cut(s) 737, 1601, 2021
HpyF3I CTNAG 9 cut(s) 5, 17, 23, 84, 222, 276, 1136, 1411, 1779
HpySE526I ACGT 2 cut(s) 13, 862
Hsp92I GRCGYC 2 cut(s) 13, 1034
Hsp92II CATG 8 cut(s) 422, 712, 963, 983, 1469, 1834, 1910, 2015
HspAI GCGC 2 cut(s) 731, 1534
Kpn2I TCCGGA 1 cut(s) 232
KroI GCCGGC 1 cut(s) 271
KroNI GCCGGC 1 cut(s) 273
Ksp22I TGATCA 1 cut(s) 1741
KspAI GTTAAC 2 cut(s) 948, 1159
Kzo9I GATC 4 cut(s) 280, 565, 1741, 1909
LmnI GCTCC 5 cut(s) 162, 344, 487, 751, 1085
Lsp1109I GCAGC 5 cut(s) 100, 844, 943, 1780, 2036
LweI GCATC 9 cut(s) 397, 844, 973, 1185, 1339, 1369, 1477, 1945, 2012
MaeI CTAG 5 cut(s) 125, 830, 897, 935, 1092
MaeII ACGT 2 cut(s) 13, 862
MaeIII GTNAC 6 cut(s) 118, 216, 436, 1108, 1574, 1826
MalI GATC 4 cut(s) 282, 567, 1743, 1911
MbiI CCGCTC 1 cut(s) 157
MboI GATC 4 cut(s) 280, 565, 1741, 1909
MfeI CAATTG 1 cut(s) 1917
MflI RGATCY 1 cut(s) 280
MhlI GDGCHC 1 cut(s) 341
MluCI AATT 5 cut(s) 451, 1731, 1859, 1870, 1917
MluI ACGCGT 1 cut(s) 697
MlyI GAGTC 1 cut(s) 417
MmeI TCCRAC 3 cut(s) 60, 222, 631
MroI TCCGGA 1 cut(s) 232
MroNI GCCGGC 1 cut(s) 271
MroXI GAANNNNTTC 1 cut(s) 1667
MslI CAYNNNNRTG 2 cut(s) 72, 1686
MspA1I CMGCKG 1 cut(s) 101
MspI CCGG 8 cut(s) 233, 272, 284, 290, 304, 342, 519, 573
MspR9I CCNGG 4 cut(s) 371, 573, 913, 1631
MunI CAATTG 1 cut(s) 1917
MvaI CCWGG 3 cut(s) 371, 913, 1631
MvnI CGCG 1 cut(s) 699
MwoI GCNNNNNNNGC 3 cut(s) 737, 1601, 2021
NaeI GCCGGC 1 cut(s) 273
NciI CCSGG 1 cut(s) 573
NcoI CCATGG 2 cut(s) 418, 708
NdeI CATATG 1 cut(s) 1687
NdeII GATC 4 cut(s) 280, 565, 1741, 1909
NgoMIV GCCGGC 1 cut(s) 271
NlaIII CATG 8 cut(s) 422, 712, 963, 983, 1469, 1834, 1910, 2015
NlaIV GGNNCC 7 cut(s) 237, 282, 288, 294, 747, 810, 927
NmuCI GTSAC 2 cut(s) 216, 1108
NspI RCATGY 1 cut(s) 2015
PaeI GCATGC 1 cut(s) 2015
PagI TCATGA 1 cut(s) 979
PceI AGGCCT 2 cut(s) 721, 1985
PcsI WCGNNNNNNNCGW 3 cut(s) 177, 186, 195
PdiI GCCGGC 1 cut(s) 273
PdmI GAANNNNTTC 1 cut(s) 1667
PfeI GAWTC 5 cut(s) 511, 815, 1121, 1270, 1415
PfoI TCCNGGA 1 cut(s) 571
PkrI GCNGC 7 cut(s) 90, 309, 646, 859, 958, 1795, 2026
PleI GAGTC 1 cut(s) 417
PpsI GAGTC 1 cut(s) 417
Psp124BI GAGCTC 1 cut(s) 341
Psp6I CCWGG 3 cut(s) 369, 911, 1629
PspGI CCWGG 3 cut(s) 369, 911, 1629
PspN4I GGNNCC 7 cut(s) 237, 282, 288, 294, 747, 810, 927
PspPI GGNCC 2 cut(s) 575, 808
PstI CTGCAG 1 cut(s) 2026
PstNI CAGNNNCTG 1 cut(s) 1112
PsuI RGATCY 1 cut(s) 280
PvuII CAGCTG 1 cut(s) 101
RsaI GTAC 3 cut(s) 1314, 1755, 1945
RsaNI GTAC 3 cut(s) 1313, 1754, 1944
RseI CAYNNNNRTG 2 cut(s) 72, 1686
SacI GAGCTC 1 cut(s) 341
SatI GCNGC 7 cut(s) 89, 308, 645, 858, 957, 1794, 2025
Sau3AI GATC 4 cut(s) 280, 565, 1741, 1909
Sau96I GGNCC 2 cut(s) 575, 808
SchI GAGTC 1 cut(s) 417
ScrFI CCNGG 4 cut(s) 371, 573, 913, 1631
SduI GDGCHC 1 cut(s) 341
SfaNI GCATC 9 cut(s) 397, 844, 973, 1185, 1339, 1369, 1477, 1945, 2012
SfcI CTRYAG 2 cut(s) 1216, 2022
SinI GGWCC 2 cut(s) 575, 808
SmiMI CAYNNNNRTG 2 cut(s) 72, 1686
SmlI CTYRAG 3 cut(s) 623, 668, 1925
SmoI CTYRAG 3 cut(s) 623, 668, 1925
SphI GCATGC 1 cut(s) 2015
Sse9I AATT 5 cut(s) 451, 1731, 1859, 1870, 1917
SseBI AGGCCT 2 cut(s) 721, 1985
SsiI CCGC 6 cut(s) 155, 269, 308, 345, 522, 645
SspMI CTAG 5 cut(s) 125, 830, 897, 935, 1092
SstI GAGCTC 1 cut(s) 341
StuI AGGCCT 2 cut(s) 721, 1985
StyD4I CCNGG 4 cut(s) 369, 571, 911, 1629
StyI CCWWGG 3 cut(s) 418, 708, 1042
TaaI ACNGT 4 cut(s) 689, 974, 1129, 2040
TaiI ACGT 2 cut(s) 16, 865
TaqI TCGA 6 cut(s) 189, 253, 564, 1309, 1371, 1530
TasI AATT 5 cut(s) 451, 1731, 1859, 1870, 1917
TatI WGTACW 2 cut(s) 1753, 1943
TauI GCSGC 2 cut(s) 310, 647
TfiI GAWTC 5 cut(s) 511, 815, 1121, 1270, 1415
TscAI CASTG 2 cut(s) 1113, 1144
TseFI GTSAC 2 cut(s) 216, 1108
TseI GCWGC 5 cut(s) 88, 857, 956, 1793, 2024
Tsp45I GTSAC 2 cut(s) 216, 1108
TspGWI ACGGA 5 cut(s) 204, 424, 807, 1072, 1814
TspRI CASTG 2 cut(s) 1113, 1144
VpaK11BI GGWCC 2 cut(s) 575, 808
XapI RAATTY 4 cut(s) 451, 1731, 1859, 1870
XbaI TCTAGA 1 cut(s) 1091
XceI RCATGY 1 cut(s) 2015
XcmI CCANNNNNNNNNTGG 1 cut(s) 919
XmnI GAANNNNTTC 1 cut(s) 1667
XspI CTAG 5 cut(s) 125, 830, 897, 935, 1092
ZraI GACGTC 1 cut(s) 14
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.