Prupe.6G312900_v2.0.a1

Homeobox protein knotted-1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0013693
Physical Location & Seq
Forward (+)
28007304 .. 28011199
3896 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G312900.1

Sequence Viewer

Length: 1005 bp
ATGAATATGGAAGATTTCTACAGGCTGAACCCAGGTGTGATATCAGGCTCAGAGATTGATGTTAATGTTGTTCATCAAGTTGAAAATATGGCTACAAGTACAAGCAATACCAGTTGTGGGTTCCATGGTCATCCTCATCCACAGGGTCATGTAGTGGATGATAACATGCTCTTGTTTCAAACGGAGGGCATGGGATCAGATAATCATATGTCTGATCTGATCAAAACCCAGATAGCCAATCACCCTCGTTTCCCTGATTTAGTTGATGCATACCTCGAATGCCAAAAGGTTGGAGCACCACTGGAGATGAAAAGTCTTCTTGAAGAAATTGGACGGGTGAGCCATCATCCCATGAGCACTTGCAGTGAGATAGGAGCTGATCCAGAACTTGACGAGTTCATGGAATCATACTGTGAGGTTCTTCGTGGATACAAGGAGGAGCTATCCAAACCGTTCGATGAAGCAACCAACTTCTTGACCAACATTCAGTCGCAACTTAGAAACCTCTGCAAAGGCACATTCCCAAAAACCAGCTGGGACTGTAATTCTGATGAAGGGGTTGGAAGTTCAGAGGAGGAATTTAGCTGTGGGGAGGTAGAAGCAGCAGAGAGTCAAGAAACAGCTGCTGCTCGTGCGGGTGGTGATCGGGAGCTGAAAGATATGCTGTTGCATAAGTACAGTGGCTATCTTACCAATTTGAGGAAGGAGTTCTTGAAGAAACGAAAGAAAGGGAAGCTACCAAAGGATGCCAGGACTGCCCTATTGGATTGGTGGACTACTCACTATAGGTGGCCATATCCTACGGAAGAGGAGAAATTGCACCTGTCTGAGGTAACTGGACTAGACCAAAAGCAGATCAACAATTGGTTCATAAACCAGAGGAAGCGACATTGGAAGCCATCTGAGGACATGAGGTTTGCTCTTATGGAGGGTGTTGGTGGCAGCATCGGAGCTCCCATGTTATTCGACGCTGGGGTCGGGACCGGAAATATTGATGATATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ELK Homeobox_KN KNOX1 KNOX2

Protein Analysis

335

Amino Acids

37.69

Weight (kDa)

5.05

Isoelectric Point (pI)

38.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013693)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g07460 FvH4_6g07460 FvH4_6g07460
malus_domestica MD04G1215500.v1.1 MD12G1205700.v1.1
prunus_persica Prupe.6G312900_v2.0.a1
pyrus_communis pycom04g17070 pycom12g19370
rosa_chinensis RchiOBHm_Chr3g0457121
rosa_laevigata RLG00000025247
rosa_multiflora Rmu_sc0000488.1_g000009
rosa_roxburghii Rroxscaffold_6G00422170
rosa_rugosa Rorug03G0018800
rosa_samantha Rh3AG078700 Rh3BG080500 Rh3CG080500 Rh3DG082300
rosa_wichuraiana Rw3G006760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 974
AciI CCGC 1 cut(s) 635
AclWI GGATC 2 cut(s) 202, 374
AcoI YGGCCR 1 cut(s) 791
AcsI RAATTY 1 cut(s) 578
AfaI GTAC 2 cut(s) 100, 677
AfiI CCNNNNNNNGG 3 cut(s) 117, 699, 829
AgsI TTSAA 4 cut(s) 83, 179, 323, 715
AjnI CCWGG 2 cut(s) 31, 749
AluBI AGCT 8 cut(s) 377, 442, 534, 585, 623, 652, 736, 953
AluI AGCT 8 cut(s) 377, 442, 534, 585, 623, 652, 736, 953
Alw21I GWGCWC 3 cut(s) 298, 359, 955
AlwI GGATC 2 cut(s) 202, 374
AlwNI CAGNNNCTG 1 cut(s) 626
AoxI GGCC 1 cut(s) 791
ApeKI GCWGC 4 cut(s) 602, 623, 626, 942
ApoI RAATTY 1 cut(s) 578
ArsI GACNNNNNNTTYG 2 cut(s) 899, 931
Asp700I GAANNNNTTC 1 cut(s) 707
AspS9I GGNCC 1 cut(s) 981
AsuHPI GGTGA 3 cut(s) 233, 349, 653
AvaII GGWCC 1 cut(s) 981
BalI TGGCCA 1 cut(s) 793
BanII GRGCYC 1 cut(s) 955
BauI CACGAG 1 cut(s) 630
BbsI GAAGAC 1 cut(s) 308
Bbv12I GWGCWC 3 cut(s) 298, 359, 955
BbvI GCAGC 4 cut(s) 610, 613, 614, 954
BccI CCATC 2 cut(s) 351, 907
BciT130I CCWGG 2 cut(s) 33, 751
BciVI GTATCC 1 cut(s) 422
BclI TGATCA 1 cut(s) 219
BfaI CTAG 1 cut(s) 842
BfmI CTRYAG 2 cut(s) 19, 784
BfuI GTATCC 1 cut(s) 422
BisI GCNGC 4 cut(s) 603, 624, 627, 943
BlsI GCNGC 4 cut(s) 604, 625, 628, 944
Bme1390I CCNGG 2 cut(s) 33, 751
Bme18I GGWCC 1 cut(s) 981
BmgT120I GGNCC 1 cut(s) 981
BmiI GGNNCC 2 cut(s) 122, 982
BmrFI CCNGG 2 cut(s) 33, 751
BmsI GCATC 3 cut(s) 256, 736, 954
BpiI GAAGAC 1 cut(s) 308
BplI GAGNNNNNCTC 2 cut(s) 904, 936
BpmI CTGGAG 1 cut(s) 323
BsaJI CCNNGG 2 cut(s) 31, 124
BsaWI WCCGGW 1 cut(s) 983
Bsc4I CCNNNNNNNGG 3 cut(s) 117, 699, 829
Bse1I ACTGG 3 cut(s) 111, 306, 841
BseBI CCWGG 2 cut(s) 33, 751
BseDI CCNNGG 2 cut(s) 31, 124
BseGI GGATG 5 cut(s) 130, 136, 163, 346, 751
BseLI CCNNNNNNNGG 3 cut(s) 117, 699, 829
BseMII CTCAG 3 cut(s) 63, 819, 894
BseNI ACTGG 3 cut(s) 111, 306, 841
BseRI GAGGAG 3 cut(s) 452, 587, 824
BseXI GCAGC 4 cut(s) 610, 613, 614, 954
BseYI CCCAGC 2 cut(s) 534, 971
BshFI GGCC 1 cut(s) 793
BsiHKAI GWGCWC 3 cut(s) 298, 359, 955
BsiSI CCGG 1 cut(s) 984
BslFI GGGAC 2 cut(s) 551, 994
BslI CCNNNNNNNGG 3 cut(s) 117, 699, 829
BsmFI GGGAC 2 cut(s) 551, 994
BsmI GAATGC 1 cut(s) 284
BsnI GGCC 1 cut(s) 793
Bsp1286I GDGCHC 3 cut(s) 298, 359, 955
Bsp143I GATC 6 cut(s) 194, 214, 219, 379, 643, 855
Bsp19I CCATGG 1 cut(s) 124
BspACI CCGC 1 cut(s) 635
BspANI GGCC 1 cut(s) 793
BspCNI CTCAG 3 cut(s) 62, 820, 895
BspLI GGNNCC 2 cut(s) 122, 982
BspPI GGATC 2 cut(s) 202, 374
BsrI ACTGG 3 cut(s) 111, 306, 841
BssECI CCNNGG 2 cut(s) 31, 124
BssMI GATC 6 cut(s) 194, 214, 219, 379, 643, 855
BssSI CACGAG 1 cut(s) 630
BssT1I CCWWGG 1 cut(s) 124
Bst2BI CACGAG 1 cut(s) 630
Bst2UI CCWGG 2 cut(s) 33, 751
Bst4CI ACNGT 4 cut(s) 413, 453, 542, 680
Bst6I CTCTTC 1 cut(s) 801
BstDEI CTNAG 4 cut(s) 49, 497, 828, 903
BstDSI CCRYGG 1 cut(s) 124
BstENI CCTNNNNNAGG 1 cut(s) 827
BstF5I GGATG 5 cut(s) 130, 136, 163, 346, 751
BstKTI GATC 6 cut(s) 197, 217, 222, 382, 646, 858
BstMBI GATC 6 cut(s) 194, 214, 219, 379, 643, 855
BstMWI GCNNNNNNNGC 2 cut(s) 632, 755
BstNI CCWGG 2 cut(s) 33, 751
BstNSI RCATGY 1 cut(s) 169
BstSCI CCNGG 2 cut(s) 31, 749
BstSFI CTRYAG 2 cut(s) 19, 784
BstV1I GCAGC 4 cut(s) 610, 613, 614, 954
BstV2I GAAGAC 1 cut(s) 308
BstXI CCANNNNNNTGG 1 cut(s) 290
BsuI GTATCC 1 cut(s) 422
BsuRI GGCC 1 cut(s) 793
BtgI CCRYGG 1 cut(s) 124
BtsCI GGATG 5 cut(s) 130, 136, 163, 346, 751
BtsI GCAGTG 1 cut(s) 370
BtsIMutI CAGTG 3 cut(s) 299, 370, 685
CaiI CAGNNNCTG 1 cut(s) 626
Cfr13I GGNCC 1 cut(s) 981
CseI GACGC 1 cut(s) 977
Csp6I GTAC 2 cut(s) 99, 676
CviAII CATG 8 cut(s) 125, 149, 166, 190, 352, 400, 910, 958
CviQI GTAC 2 cut(s) 99, 676
DdeI CTNAG 4 cut(s) 49, 497, 828, 903
DpnI GATC 6 cut(s) 196, 216, 221, 381, 645, 857
DpnII GATC 6 cut(s) 194, 214, 219, 379, 643, 855
DrdI GACNNNNNNGTC 1 cut(s) 974
DseDI GACNNNNNNGTC 1 cut(s) 974
EaeI YGGCCR 1 cut(s) 791
Eam1104I CTCTTC 1 cut(s) 801
EarI CTCTTC 1 cut(s) 801
Ecl136II GAGCTC 1 cut(s) 953
Eco130I CCWWGG 1 cut(s) 124
Eco24I GRGCYC 1 cut(s) 955
Eco32I GATATC 1 cut(s) 42
Eco47I GGWCC 1 cut(s) 981
Eco53kI GAGCTC 1 cut(s) 953
EcoICRI GAGCTC 1 cut(s) 953
EcoNI CCTNNNNNAGG 1 cut(s) 827
EcoRII CCWGG 2 cut(s) 31, 749
EcoRV GATATC 1 cut(s) 42
EcoT14I CCWWGG 1 cut(s) 124
EcoT22I ATGCAT 1 cut(s) 271
EcoT38I GRGCYC 1 cut(s) 955
ErhI CCWWGG 1 cut(s) 124
FaeI CATG 8 cut(s) 128, 152, 169, 193, 355, 403, 913, 961
FalI AAGNNNNNCTT 2 cut(s) 695, 727
FaqI GGGAC 2 cut(s) 551, 994
FatI CATG 8 cut(s) 124, 148, 165, 189, 351, 399, 909, 957
FauI CCCGC 1 cut(s) 628
FauNDI CATATG 1 cut(s) 207
FbaI TGATCA 1 cut(s) 219
Fnu4HI GCNGC 4 cut(s) 603, 624, 627, 943
FokI GGATG 5 cut(s) 117, 123, 170, 333, 758
FriOI GRGCYC 1 cut(s) 955
Fsp4HI GCNGC 4 cut(s) 603, 624, 627, 943
FspBI CTAG 1 cut(s) 842
GluI GCNGC 4 cut(s) 603, 624, 627, 943
GsaI CCCAGC 2 cut(s) 538, 975
GsuI CTGGAG 1 cut(s) 323
HaeIII GGCC 1 cut(s) 793
HapII CCGG 1 cut(s) 984
HgaI GACGC 1 cut(s) 977
Hin1II CATG 8 cut(s) 128, 152, 169, 193, 355, 403, 913, 961
HinfI GANTC 2 cut(s) 404, 610
HpaII CCGG 1 cut(s) 984
HphI GGTGA 3 cut(s) 233, 349, 653
Hpy166II GTNNAC 1 cut(s) 774
Hpy188I TCNGA 9 cut(s) 52, 199, 214, 219, 550, 571, 829, 904, 950
Hpy188III TCNNGA 7 cut(s) 320, 383, 475, 614, 647, 712, 979
Hpy8I GTNNAC 1 cut(s) 774
Hpy99I CGWCG 1 cut(s) 971
HpyAV CCTTC 2 cut(s) 548, 697
HpyCH4III ACNGT 4 cut(s) 413, 453, 542, 680
HpyCH4V TGCA 5 cut(s) 269, 363, 510, 670, 820
HpyF10VI GCNNNNNNNGC 2 cut(s) 632, 755
HpyF3I CTNAG 4 cut(s) 49, 497, 828, 903
Hsp92II CATG 8 cut(s) 128, 152, 169, 193, 355, 403, 913, 961
Ksp22I TGATCA 1 cut(s) 219
Kzo9I GATC 6 cut(s) 194, 214, 219, 379, 643, 855
LmnI GCTCC 6 cut(s) 293, 374, 439, 649, 950, 958
Lsp1109I GCAGC 4 cut(s) 610, 613, 614, 954
LweI GCATC 3 cut(s) 256, 736, 954
MaeI CTAG 1 cut(s) 842
MaeIII GTNAC 1 cut(s) 832
MalI GATC 6 cut(s) 196, 216, 221, 381, 645, 857
MboI GATC 6 cut(s) 194, 214, 219, 379, 643, 855
MboII GAAGA 6 cut(s) 23, 308, 335, 413, 727, 818
MfeI CAATTG 1 cut(s) 862
MhlI GDGCHC 3 cut(s) 298, 359, 955
MlsI TGGCCA 1 cut(s) 793
MluCI AATT 6 cut(s) 327, 544, 578, 694, 815, 862
MluNI TGGCCA 1 cut(s) 793
MlyI GAGTC 1 cut(s) 619
MmeI TCCRAC 2 cut(s) 271, 541
Mox20I TGGCCA 1 cut(s) 793
Mph1103I ATGCAT 1 cut(s) 271
MroXI GAANNNNTTC 1 cut(s) 707
MscI TGGCCA 1 cut(s) 793
MseI TTAA 1 cut(s) 63
Msp20I TGGCCA 1 cut(s) 793
MspA1I CMGCKG 2 cut(s) 534, 623
MspI CCGG 1 cut(s) 984
MspR9I CCNGG 2 cut(s) 33, 751
MunI CAATTG 1 cut(s) 862
Mva1269I GAATGC 1 cut(s) 284
MvaI CCWGG 2 cut(s) 33, 751
MwoI GCNNNNNNNGC 2 cut(s) 632, 755
NcoI CCATGG 1 cut(s) 124
NdeI CATATG 1 cut(s) 207
NdeII GATC 6 cut(s) 194, 214, 219, 379, 643, 855
NlaIII CATG 8 cut(s) 128, 152, 169, 193, 355, 403, 913, 961
NlaIV GGNNCC 2 cut(s) 122, 982
NsiI ATGCAT 1 cut(s) 271
NspI RCATGY 1 cut(s) 169
PctI GAATGC 1 cut(s) 284
PdmI GAANNNNTTC 1 cut(s) 707
PfeI GAWTC 1 cut(s) 404
PkrI GCNGC 4 cut(s) 604, 625, 628, 944
PleI GAGTC 1 cut(s) 618
PpsI GAGTC 1 cut(s) 618
Psp124BI GAGCTC 1 cut(s) 955
Psp6I CCWGG 2 cut(s) 31, 749
PspFI CCCAGC 2 cut(s) 534, 971
PspGI CCWGG 2 cut(s) 31, 749
PspN4I GGNNCC 2 cut(s) 122, 982
PspPI GGNCC 1 cut(s) 981
PstNI CAGNNNCTG 1 cut(s) 626
PvuII CAGCTG 2 cut(s) 534, 623
RsaI GTAC 2 cut(s) 100, 677
RsaNI GTAC 2 cut(s) 99, 676
SacI GAGCTC 1 cut(s) 955
SaqAI TTAA 1 cut(s) 63
SatI GCNGC 4 cut(s) 603, 624, 627, 943
Sau3AI GATC 6 cut(s) 194, 214, 219, 379, 643, 855
Sau96I GGNCC 1 cut(s) 981
SchI GAGTC 1 cut(s) 619
ScrFI CCNGG 2 cut(s) 33, 751
SduI GDGCHC 3 cut(s) 298, 359, 955
SfaNI GCATC 3 cut(s) 256, 736, 954
SfcI CTRYAG 2 cut(s) 19, 784
SinI GGWCC 1 cut(s) 981
Sse9I AATT 6 cut(s) 327, 544, 578, 694, 815, 862
SsiI CCGC 1 cut(s) 635
SspI AATATT 1 cut(s) 991
SspMI CTAG 1 cut(s) 842
SstI GAGCTC 1 cut(s) 955
StyD4I CCNGG 2 cut(s) 31, 749
StyI CCWWGG 1 cut(s) 124
TaaI ACNGT 4 cut(s) 413, 453, 542, 680
TaqI TCGA 3 cut(s) 276, 456, 966
TasI AATT 6 cut(s) 327, 544, 578, 694, 815, 862
TatI WGTACW 2 cut(s) 98, 675
TfiI GAWTC 1 cut(s) 404
Tru1I TTAA 1 cut(s) 63
Tru9I TTAA 1 cut(s) 63
TscAI CASTG 3 cut(s) 306, 370, 685
TseI GCWGC 4 cut(s) 602, 623, 626, 942
TspDTI ATGAA 7 cut(s) 17, 62, 323, 388, 474, 567, 859
TspGWI ACGGA 2 cut(s) 197, 818
TspRI CASTG 3 cut(s) 306, 370, 685
VpaK11BI GGWCC 1 cut(s) 981
XagI CCTNNNNNAGG 1 cut(s) 827
XapI RAATTY 1 cut(s) 578
XceI RCATGY 1 cut(s) 169
XcmI CCANNNNNNNNNTGG 1 cut(s) 531
XmnI GAANNNNTTC 1 cut(s) 707
XspI CTAG 1 cut(s) 842
Zsp2I ATGCAT 1 cut(s) 271
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.