pycom12g19370

Homeobox protein knotted-1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0013693
Physical Location & Seq
Forward (+)
21232295 .. 21235172
2878 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g19370.3

Sequence Viewer

Length: 792 bp
ATGGTTTGGGATCCGATCGATCATATGTCTGATCTGATAAAGTCCCAGATAGCCAGTCATCCTCGTTTCCCTGATTTAGTAGCTGCATACCTTGAATGCCAAAAGGTTGGAGCACCAGCGGAGATGAAAAATCTGCTTGAAGAAATTGAAAGGGCAAGCCACCCCATGAGCACTTGCAGTGAGATAGGAGCTGATCCTGAACTTGACGAGTTCATGGAATCATATATTGAGGTTCTCCATAGATACAAGGAGGAGCTATCTAAACCATTCGATGAAGCAACCACATTCTTGACCAACATTCAAACTCAACTTAGCAACCTCTGTAAAGGGACATTCTCAAAAACCTGGGACTATCACTCTGATGAAGGGGTCGGCAGTTCGGAGGAGGAACTTAGCTGTGGGGAGGTGGAAGCAGCAGAGGGTCAGGACACTGGTGGTGCTCGTTCTAGTGGTGATCGGGAACTGAAAGATATGCTGCTGAATAAGTACAGTGGCTACCTTAGCAGCTTGAGGAAGGAGTTCTTAAAGAAAAGAAAGAAAGGGAAACTACCAAAGGATGCCAGGAGTACCCTATCGGACTGGTGGATTACTCACTATAGGTGGCCATATCCTACGGAAGAGGAGAAACTCCAGCTGTCTGAGATGACTGGATTGGACCAAAAGCAGATTAACAACTGGTTCATAAACCAGAGGAAGAGGCATTGGAAACCATCTGAGGACATGAGGTTTGCTCTCAATGAAGGTGTCGGCGGGAGCATCGGAGGACCTATGATGTTTGACACTGGTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ELK Homeobox_KN KNOX1 KNOX2

Protein Analysis

264

Amino Acids

30.04

Weight (kDa)

5.08

Isoelectric Point (pI)

47.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013693)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g07460 FvH4_6g07460 FvH4_6g07460
malus_domestica MD04G1215500.v1.1 MD12G1205700.v1.1
prunus_persica Prupe.6G312900_v2.0.a1
pyrus_communis pycom04g17070 pycom12g19370
rosa_chinensis RchiOBHm_Chr3g0457121
rosa_laevigata RLG00000025247
rosa_multiflora Rmu_sc0000488.1_g000009
rosa_roxburghii Rroxscaffold_6G00422170
rosa_rugosa Rorug03G0018800
rosa_samantha Rh3AG078700 Rh3BG080500 Rh3CG080500 Rh3DG082300
rosa_wichuraiana Rw3G006760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 119, 750
AclWI GGATC 3 cut(s) 5, 18, 188
AcoI YGGCCR 1 cut(s) 602
AfaI GTAC 2 cut(s) 488, 568
AgsI TTSAA 4 cut(s) 95, 140, 149, 302
AjnI CCWGG 2 cut(s) 344, 560
AluBI AGCT 6 cut(s) 83, 191, 256, 396, 507, 634
AluI AGCT 6 cut(s) 83, 191, 256, 396, 507, 634
Alw21I GWGCWC 3 cut(s) 115, 173, 442
AlwI GGATC 3 cut(s) 5, 18, 188
AoxI GGCC 1 cut(s) 602
ApeKI GCWGC 4 cut(s) 83, 413, 475, 504
ArsI GACNNNNNNTTYG 2 cut(s) 710, 742
Asp700I GAANNNNTTC 1 cut(s) 518
AspS9I GGNCC 2 cut(s) 655, 764
AsuHPI GGTGA 1 cut(s) 464
AvaII GGWCC 2 cut(s) 655, 764
BalI TGGCCA 1 cut(s) 604
BamHI GGATCC 1 cut(s) 10
Bbv12I GWGCWC 3 cut(s) 115, 173, 442
BbvI GCAGC 4 cut(s) 70, 425, 462, 516
BccI CCATC 1 cut(s) 718
BciT130I CCWGG 2 cut(s) 346, 562
BfaI CTAG 1 cut(s) 447
BfmI CTRYAG 1 cut(s) 595
BisI GCNGC 4 cut(s) 84, 414, 476, 505
BlsI GCNGC 4 cut(s) 85, 415, 477, 506
Bme1390I CCNGG 2 cut(s) 346, 562
Bme18I GGWCC 2 cut(s) 655, 764
BmgT120I GGNCC 2 cut(s) 655, 764
BmiI GGNNCC 1 cut(s) 12
BmrFI CCNGG 2 cut(s) 346, 562
BmsI GCATC 2 cut(s) 547, 765
BplI GAGNNNNNCTC 2 cut(s) 715, 747
BpmI CTGGAG 1 cut(s) 614
Bpu10I CCTNAGC 1 cut(s) 500
BpuEI CTTGAG 1 cut(s) 529
Bsa29I ATCGAT 1 cut(s) 18
BsaJI CCNNGG 1 cut(s) 345
Bse1I ACTGG 6 cut(s) 54, 436, 584, 652, 680, 787
BseBI CCWGG 2 cut(s) 346, 562
BseCI ATCGAT 1 cut(s) 18
BseDI CCNNGG 1 cut(s) 345
BseGI GGATG 2 cut(s) 58, 562
BseMII CTCAG 2 cut(s) 630, 705
BseNI ACTGG 6 cut(s) 54, 436, 584, 652, 680, 787
BseRI GAGGAG 3 cut(s) 266, 398, 635
BseXI GCAGC 4 cut(s) 70, 425, 462, 516
Bsh1285I CGRYCG 1 cut(s) 18
BshFI GGCC 1 cut(s) 604
BshVI ATCGAT 1 cut(s) 18
BsiEI CGRYCG 1 cut(s) 18
BsiHKAI GWGCWC 3 cut(s) 115, 173, 442
BslFI GGGAC 3 cut(s) 28, 343, 362
BsmFI GGGAC 3 cut(s) 28, 343, 362
BsmI GAATGC 1 cut(s) 101
BsnI GGCC 1 cut(s) 604
Bsp1286I GDGCHC 3 cut(s) 115, 173, 442
Bsp143I GATC 6 cut(s) 10, 15, 19, 31, 193, 454
BspACI CCGC 2 cut(s) 119, 750
BspANI GGCC 1 cut(s) 604
BspCNI CTCAG 2 cut(s) 631, 706
BspDI ATCGAT 1 cut(s) 18
BspLI GGNNCC 1 cut(s) 12
BspPI GGATC 3 cut(s) 5, 18, 188
BsrI ACTGG 6 cut(s) 54, 436, 584, 652, 680, 787
BssECI CCNNGG 1 cut(s) 345
BssMI GATC 6 cut(s) 10, 15, 19, 31, 193, 454
Bst2UI CCWGG 2 cut(s) 346, 562
Bst4CI ACNGT 1 cut(s) 491
Bst6I CTCTTC 2 cut(s) 612, 689
BstC8I GCNNGC 1 cut(s) 157
BstDEI CTNAG 5 cut(s) 311, 392, 500, 639, 714
BstF5I GGATG 2 cut(s) 58, 562
BstKTI GATC 6 cut(s) 13, 18, 22, 34, 196, 457
BstMBI GATC 6 cut(s) 10, 15, 19, 31, 193, 454
BstMCI CGRYCG 1 cut(s) 18
BstMWI GCNNNNNNNGC 1 cut(s) 501
BstNI CCWGG 2 cut(s) 346, 562
BstSCI CCNGG 2 cut(s) 344, 560
BstSFI CTRYAG 1 cut(s) 595
BstV1I GCAGC 4 cut(s) 70, 425, 462, 516
BstX2I RGATCY 1 cut(s) 10
BstXI CCANNNNNNTGG 1 cut(s) 107
BstYI RGATCY 1 cut(s) 10
Bsu15I ATCGAT 1 cut(s) 18
BsuRI GGCC 1 cut(s) 604
BsuTUI ATCGAT 1 cut(s) 18
BtsCI GGATG 2 cut(s) 58, 562
BtsI GCAGTG 1 cut(s) 184
BtsIMutI CAGTG 4 cut(s) 184, 429, 496, 780
Cac8I GCNNGC 1 cut(s) 157
Cfr13I GGNCC 2 cut(s) 655, 764
ClaI ATCGAT 1 cut(s) 18
Csp6I GTAC 2 cut(s) 487, 567
CviAII CATG 3 cut(s) 166, 214, 721
CviQI GTAC 2 cut(s) 487, 567
DdeI CTNAG 5 cut(s) 311, 392, 500, 639, 714
DpnI GATC 6 cut(s) 12, 17, 21, 33, 195, 456
DpnII GATC 6 cut(s) 10, 15, 19, 31, 193, 454
EaeI YGGCCR 1 cut(s) 602
Eam1104I CTCTTC 2 cut(s) 612, 689
EarI CTCTTC 2 cut(s) 612, 689
Eco47I GGWCC 2 cut(s) 655, 764
EcoO109I RGGNCCY 1 cut(s) 764
EcoRII CCWGG 2 cut(s) 344, 560
FaeI CATG 3 cut(s) 169, 217, 724
FalI AAGNNNNNCTT 2 cut(s) 506, 538
FaqI GGGAC 3 cut(s) 28, 343, 362
FatI CATG 3 cut(s) 165, 213, 720
FauI CCCGC 1 cut(s) 743
FauNDI CATATG 1 cut(s) 24
Fnu4HI GCNGC 4 cut(s) 84, 414, 476, 505
FokI GGATG 2 cut(s) 45, 569
Fsp4HI GCNGC 4 cut(s) 84, 414, 476, 505
FspBI CTAG 1 cut(s) 447
GluI GCNGC 4 cut(s) 84, 414, 476, 505
GsuI CTGGAG 1 cut(s) 614
HaeIII GGCC 1 cut(s) 604
Hin1II CATG 3 cut(s) 169, 217, 724
HinfI GANTC 1 cut(s) 218
HphI GGTGA 1 cut(s) 464
Hpy188I TCNGA 9 cut(s) 15, 31, 36, 361, 382, 577, 640, 715, 761
Hpy188III TCNNGA 4 cut(s) 197, 289, 425, 458
HpyAV CCTTC 3 cut(s) 359, 508, 734
HpyCH4III ACNGT 1 cut(s) 491
HpyCH4V TGCA 2 cut(s) 86, 177
HpyF10VI GCNNNNNNNGC 1 cut(s) 501
HpyF3I CTNAG 5 cut(s) 311, 392, 500, 639, 714
Hsp92II CATG 3 cut(s) 169, 217, 724
Kzo9I GATC 6 cut(s) 10, 15, 19, 31, 193, 454
LmnI GCTCC 4 cut(s) 110, 188, 253, 753
Lsp1109I GCAGC 4 cut(s) 70, 425, 462, 516
LweI GCATC 2 cut(s) 547, 765
MaeI CTAG 1 cut(s) 447
MalI GATC 6 cut(s) 12, 17, 21, 33, 195, 456
MboI GATC 6 cut(s) 10, 15, 19, 31, 193, 454
MboII GAAGA 3 cut(s) 152, 629, 706
MflI RGATCY 1 cut(s) 10
MhlI GDGCHC 3 cut(s) 115, 173, 442
MlsI TGGCCA 1 cut(s) 604
MluCI AATT 1 cut(s) 144
MluNI TGGCCA 1 cut(s) 604
MmeI TCCRAC 1 cut(s) 88
Mox20I TGGCCA 1 cut(s) 604
MroXI GAANNNNTTC 1 cut(s) 518
MscI TGGCCA 1 cut(s) 604
MseI TTAA 2 cut(s) 524, 669
MslI CAYNNNNRTG 1 cut(s) 360
Msp20I TGGCCA 1 cut(s) 604
MspA1I CMGCKG 2 cut(s) 119, 634
MspR9I CCNGG 2 cut(s) 346, 562
Mva1269I GAATGC 1 cut(s) 101
MvaI CCWGG 2 cut(s) 346, 562
MwoI GCNNNNNNNGC 1 cut(s) 501
NdeI CATATG 1 cut(s) 24
NdeII GATC 6 cut(s) 10, 15, 19, 31, 193, 454
NlaIII CATG 3 cut(s) 169, 217, 724
NlaIV GGNNCC 1 cut(s) 12
PctI GAATGC 1 cut(s) 101
PdmI GAANNNNTTC 1 cut(s) 518
PfeI GAWTC 1 cut(s) 218
PkrI GCNGC 4 cut(s) 85, 415, 477, 506
Ple19I CGATCG 1 cut(s) 18
PpuMI RGGWCCY 1 cut(s) 764
Psp5II RGGWCCY 1 cut(s) 764
Psp6I CCWGG 2 cut(s) 344, 560
PspGI CCWGG 2 cut(s) 344, 560
PspN4I GGNNCC 1 cut(s) 12
PspPI GGNCC 2 cut(s) 655, 764
PspPPI RGGWCCY 1 cut(s) 764
PsuI RGATCY 1 cut(s) 10
PvuI CGATCG 1 cut(s) 18
PvuII CAGCTG 1 cut(s) 634
RsaI GTAC 2 cut(s) 488, 568
RsaNI GTAC 2 cut(s) 487, 567
RseI CAYNNNNRTG 1 cut(s) 360
SaqAI TTAA 2 cut(s) 524, 669
SatI GCNGC 4 cut(s) 84, 414, 476, 505
Sau3AI GATC 6 cut(s) 10, 15, 19, 31, 193, 454
Sau96I GGNCC 2 cut(s) 655, 764
ScrFI CCNGG 2 cut(s) 346, 562
SduI GDGCHC 3 cut(s) 115, 173, 442
SfaNI GCATC 2 cut(s) 547, 765
SfcI CTRYAG 1 cut(s) 595
SinI GGWCC 2 cut(s) 655, 764
SmiMI CAYNNNNRTG 1 cut(s) 360
SmlI CTYRAG 1 cut(s) 508
SmoI CTYRAG 1 cut(s) 508
Sse9I AATT 1 cut(s) 144
SsiI CCGC 2 cut(s) 119, 750
SspMI CTAG 1 cut(s) 447
StyD4I CCNGG 2 cut(s) 344, 560
TaaI ACNGT 1 cut(s) 491
TaqI TCGA 2 cut(s) 18, 270
TasI AATT 1 cut(s) 144
TatI WGTACW 1 cut(s) 486
TfiI GAWTC 1 cut(s) 218
Tru1I TTAA 2 cut(s) 524, 669
Tru9I TTAA 2 cut(s) 524, 669
TscAI CASTG 4 cut(s) 184, 436, 496, 787
TseI GCWGC 4 cut(s) 83, 413, 475, 504
TspDTI ATGAA 6 cut(s) 140, 202, 288, 378, 670, 753
TspGWI ACGGA 1 cut(s) 629
TspRI CASTG 4 cut(s) 184, 436, 496, 787
VpaK11BI GGWCC 2 cut(s) 655, 764
XmnI GAANNNNTTC 1 cut(s) 518
XspI CTAG 1 cut(s) 447
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.