pycom01g03820

spliceosomal complex assembly

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0019313
Physical Location & Seq
Forward (+)
2872394 .. 2874012
1619 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g03820.1

Sequence Viewer

Length: 1248 bp
ATGTGTAGTATCTTGATAGTGGGTGCTCTCATTCACATGACTGGAGACAATGAGACTTTCTCATCTCTTGCTCCTTTTATTGGTAGTGGTGTTGTGTTTGGTGGTGGAGACAAGTCTCAGATCACAGGGAAAGGTGATGTCAACCTCCGATCGATCCTTGGATTACCTAAGCTTGAAAATGTTGGCTATGTTGATGGATTGAAGTCAAATATCATTAGCATTAGCCATTTGTGTGATGATGTGGTTGGTGAAGTGTGCTTCTCAAAACAAGGTTGCTGGATTGTGGATGAACATGGAAAGAACTTGCTTGTACTACCGAGATCTGGAGACAGTTGCTACATTCTTGATGTCTGTAAAGTTGCTGAATACTCCAAGTGCCATAAAGCTATCGATGAAACTAGTGTATTATGGCATGATAGGATTAAAAGCACACCACAAAGTAATTTGTCAATTGTAGCATATGAAAATAAGGGTATTTCCCACAGAAGATTGTTTTATCTAACTACTTTAAATGTCACAAAAACTGGGTTGCTGTCCTTACTGACCATCTACGAACTTTTATATGCAAACACTAGACGCACAAAGCTACACACACACACACACACACACACACACACACACGGACATAAATATTTGGGATTTTTGGAGGGAATTGCTATCCACCACCAAATAATCATGCAAACATGTTATGTTTCATTCAAATTCTTTTTATTTCCGGATGAAGATGTCAATATGTTAAGAGAATGGCGTTTTCAACTTAACTTAGTTCCTAGCATGCAATCTAGAATGGCGTGTTCATCACGAGCCATCGTAAGTACTAGCGTTGTCTTACTTATCCTAGAAATTGGTTCACATGTTAATCTTGACAATGGCAGGCACACACAAATTCATAGCATTAGAATTCTAGATATGCTTACTAAGTATTTTCATCCATTCATAAAAGTAATTCAAACAAAATGTCATAACAAACTTGCAATCATATTCGGGGTTTCAAAACAGCCCCTAACTACTAAAAATTTAGTTACACATAATGAGGCTTCCTCTTGCTTCCTTCCCCAACACTGCTGCCTTGCCTTTTTTCTTCATTTCCGCTGCAACTTGCAGGTATTTTTTTTTTCAAAATTATTTTTGAAAAAAAAATTGCGCTTTATTTCCTCCCTCAACACTCCTTTAAACCAACACTTGCTGCATCATTTCCATAATTGGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCACCAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2 gag_pre-integrs rve zf-CCHC

Protein Analysis

416

Amino Acids

46.92

Weight (kDa)

9.06

Isoelectric Point (pI)

39.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pol_BBD PF22936 8 - 79 4.9e-12 Pol polyprotein, beta-barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1093
AccIII TCCGGA 1 cut(s) 715
AciI CCGC 1 cut(s) 1090
AclWI GGATC 1 cut(s) 148
AcsI RAATTY 4 cut(s) 701, 885, 900, 1015
AfaI GTAC 2 cut(s) 312, 817
AfiI CCNNNNNNNGG 2 cut(s) 80, 323
AflIII ACRYGT 2 cut(s) 683, 853
AgsI TTSAA 8 cut(s) 176, 202, 700, 755, 950, 993, 1119, 1132
AhlI ACTAGT 1 cut(s) 398
AluBI AGCT 3 cut(s) 172, 386, 586
AluI AGCT 3 cut(s) 172, 386, 586
Alw21I GWGCWC 1 cut(s) 28
Alw26I GTCTC 5 cut(s) 39, 47, 102, 120, 321
AlwI GGATC 1 cut(s) 148
Aor13HI TCCGGA 1 cut(s) 715
AoxI GGCC 1 cut(s) 1205
ApeKI GCWGC 3 cut(s) 1065, 1092, 1186
ApoI RAATTY 4 cut(s) 701, 885, 900, 1015
ArsI GACNNNNNNTTYG 2 cut(s) 943, 975
AspLEI GCGC 1 cut(s) 1146
AspS9I GGNCC 1 cut(s) 1206
AsuHPI GGTGA 2 cut(s) 146, 260
BauI CACGAG 1 cut(s) 801
Bbv12I GWGCWC 1 cut(s) 28
BbvI GCAGC 3 cut(s) 1052, 1079, 1173
BccI CCATC 3 cut(s) 188, 554, 815
BcoDI GTCTC 5 cut(s) 39, 47, 102, 120, 321
BcuI ACTAGT 1 cut(s) 398
BfaI CTAG 7 cut(s) 399, 573, 771, 783, 819, 839, 905
BfuAI ACCTGC 1 cut(s) 1093
BglII AGATCT 1 cut(s) 320
BisI GCNGC 3 cut(s) 1066, 1093, 1187
BlsI GCNGC 3 cut(s) 1067, 1094, 1188
BmcAI AGTACT 1 cut(s) 817
BmgT120I GGNCC 1 cut(s) 1206
BmiI GGNNCC 1 cut(s) 1208
BmrI ACTGGG 1 cut(s) 534
BmsI GCATC 1 cut(s) 1198
BmuI ACTGGG 1 cut(s) 534
BplI GAGNNNNNCTC 4 cut(s) 44, 76, 1025, 1057
BpmI CTGGAG 2 cut(s) 63, 345
Bpu10I CCTNAGC 1 cut(s) 168
Bsa29I ATCGAT 2 cut(s) 152, 390
BsaJI CCNNGG 1 cut(s) 157
BsaWI WCCGGW 1 cut(s) 715
Bsc4I CCNNNNNNNGG 2 cut(s) 80, 323
Bse1I ACTGG 2 cut(s) 46, 529
BseAI TCCGGA 1 cut(s) 715
BseCI ATCGAT 2 cut(s) 152, 390
BseDI CCNNGG 1 cut(s) 157
BseGI GGATG 3 cut(s) 292, 724, 928
BseLI CCNNNNNNNGG 2 cut(s) 80, 323
BseMII CTCAG 1 cut(s) 131
BseNI ACTGG 2 cut(s) 46, 529
BseXI GCAGC 3 cut(s) 1052, 1079, 1173
Bsh1285I CGRYCG 1 cut(s) 152
BshFI GGCC 1 cut(s) 1207
BshVI ATCGAT 2 cut(s) 152, 390
BsiEI CGRYCG 1 cut(s) 152
BsiHKAI GWGCWC 1 cut(s) 28
BsiSI CCGG 1 cut(s) 716
BslI CCNNNNNNNGG 2 cut(s) 80, 323
BsmAI GTCTC 5 cut(s) 39, 47, 102, 120, 321
BsnI GGCC 1 cut(s) 1207
Bsp1286I GDGCHC 1 cut(s) 28
Bsp13I TCCGGA 1 cut(s) 715
Bsp143I GATC 4 cut(s) 120, 149, 153, 320
BspACI CCGC 1 cut(s) 1090
BspANI GGCC 1 cut(s) 1207
BspCNI CTCAG 1 cut(s) 130
BspDI ATCGAT 2 cut(s) 152, 390
BspEI TCCGGA 1 cut(s) 715
BspLI GGNNCC 1 cut(s) 1208
BspMI ACCTGC 1 cut(s) 1093
BspPI GGATC 1 cut(s) 148
BsrI ACTGG 2 cut(s) 46, 529
BssECI CCNNGG 1 cut(s) 157
BssMI GATC 4 cut(s) 120, 149, 153, 320
BssSI CACGAG 1 cut(s) 801
BssT1I CCWWGG 1 cut(s) 157
Bst2BI CACGAG 1 cut(s) 801
Bst4CI ACNGT 1 cut(s) 332
BstC8I GCNNGC 2 cut(s) 776, 875
BstDEI CTNAG 4 cut(s) 117, 168, 763, 918
BstF5I GGATG 3 cut(s) 292, 724, 928
BstHHI GCGC 1 cut(s) 1146
BstKTI GATC 4 cut(s) 123, 152, 156, 323
BstMAI GTCTC 5 cut(s) 39, 47, 102, 120, 321
BstMBI GATC 4 cut(s) 120, 149, 153, 320
BstMCI CGRYCG 1 cut(s) 152
BstNSI RCATGY 3 cut(s) 687, 778, 857
BstV1I GCAGC 3 cut(s) 1052, 1079, 1173
BstX2I RGATCY 1 cut(s) 320
BstYI RGATCY 1 cut(s) 320
Bsu15I ATCGAT 2 cut(s) 152, 390
BsuRI GGCC 1 cut(s) 1207
BsuTUI ATCGAT 2 cut(s) 152, 390
BtsCI GGATG 3 cut(s) 292, 724, 928
BtsI GCAGTG 1 cut(s) 1060
BtsIMutI CAGTG 1 cut(s) 1060
BveI ACCTGC 1 cut(s) 1093
Cac8I GCNNGC 2 cut(s) 776, 875
CfoI GCGC 1 cut(s) 1146
Cfr13I GGNCC 1 cut(s) 1206
ClaI ATCGAT 2 cut(s) 152, 390
CseI GACGC 1 cut(s) 585
Csp6I GTAC 2 cut(s) 311, 816
CviAII CATG 7 cut(s) 37, 293, 413, 676, 684, 775, 854
CviJI RGCY 9 cut(s) 172, 186, 225, 386, 586, 806, 1000, 1037, 1207
CviKI_1 RGCY 9 cut(s) 172, 186, 225, 386, 586, 806, 1000, 1037, 1207
CviQI GTAC 2 cut(s) 311, 816
DdeI CTNAG 4 cut(s) 117, 168, 763, 918
DpnI GATC 4 cut(s) 122, 151, 155, 322
DpnII GATC 4 cut(s) 120, 149, 153, 320
DraI TTTAAA 2 cut(s) 510, 1173
Eco130I CCWWGG 1 cut(s) 157
EcoRI GAATTC 1 cut(s) 900
EcoT14I CCWWGG 1 cut(s) 157
ErhI CCWWGG 1 cut(s) 157
FaeI CATG 7 cut(s) 40, 296, 416, 679, 687, 778, 857
FatI CATG 7 cut(s) 36, 292, 412, 675, 683, 774, 853
FauNDI CATATG 1 cut(s) 460
Fnu4HI GCNGC 3 cut(s) 1066, 1093, 1187
FokI GGATG 3 cut(s) 299, 731, 915
Fsp4HI GCNGC 3 cut(s) 1066, 1093, 1187
FspBI CTAG 7 cut(s) 399, 573, 771, 783, 819, 839, 905
GlaI GCGC 1 cut(s) 1145
GluI GCNGC 3 cut(s) 1066, 1093, 1187
GsuI CTGGAG 2 cut(s) 63, 345
HaeIII GGCC 1 cut(s) 1207
HapII CCGG 1 cut(s) 716
HgaI GACGC 1 cut(s) 585
HhaI GCGC 1 cut(s) 1146
Hin1II CATG 7 cut(s) 40, 296, 416, 679, 687, 778, 857
Hin6I GCGC 1 cut(s) 1144
HinP1I GCGC 1 cut(s) 1144
HincII GTYRAC 1 cut(s) 142
HindII GTYRAC 1 cut(s) 142
HindIII AAGCTT 1 cut(s) 170
HpaII CCGG 1 cut(s) 716
HphI GGTGA 2 cut(s) 146, 260
Hpy166II GTNNAC 2 cut(s) 142, 851
Hpy188I TCNGA 2 cut(s) 120, 149
Hpy188III TCNNGA 8 cut(s) 13, 324, 344, 716, 783, 801, 863, 905
Hpy8I GTNNAC 2 cut(s) 142, 851
HpyAV CCTTC 1 cut(s) 1061
HpyCH4III ACNGT 1 cut(s) 332
HpyCH4V TGCA 7 cut(s) 566, 679, 778, 974, 1095, 1102, 1189
HpyF3I CTNAG 4 cut(s) 117, 168, 763, 918
Hsp92II CATG 7 cut(s) 40, 296, 416, 679, 687, 778, 857
HspAI GCGC 1 cut(s) 1144
Kpn2I TCCGGA 1 cut(s) 715
Kzo9I GATC 4 cut(s) 120, 149, 153, 320
LmnI GCTCC 1 cut(s) 76
LpnPI CCDG 8 cut(s) 27, 111, 262, 309, 510, 729, 859, 1088
Lsp1109I GCAGC 3 cut(s) 1052, 1079, 1173
LweI GCATC 1 cut(s) 1198
MaeI CTAG 7 cut(s) 399, 573, 771, 783, 819, 839, 905
MaeIII GTNAC 2 cut(s) 514, 1021
MalI GATC 4 cut(s) 122, 151, 155, 322
MboI GATC 4 cut(s) 120, 149, 153, 320
MboII GAAGA 3 cut(s) 498, 734, 1073
MfeI CAATTG 1 cut(s) 450
MflI RGATCY 1 cut(s) 320
MhlI GDGCHC 1 cut(s) 28
MnlI CCTC 6 cut(s) 155, 640, 1027, 1051, 1165, 1169
MroI TCCGGA 1 cut(s) 715
MseI TTAA 6 cut(s) 423, 509, 737, 759, 858, 1172
MslI CAYNNNNRTG 2 cut(s) 35, 231
MspA1I CMGCKG 1 cut(s) 1092
MspI CCGG 1 cut(s) 716
MunI CAATTG 1 cut(s) 450
NdeI CATATG 1 cut(s) 460
NdeII GATC 4 cut(s) 120, 149, 153, 320
NlaIII CATG 7 cut(s) 40, 296, 416, 679, 687, 778, 857
NlaIV GGNNCC 1 cut(s) 1208
NmuCI GTSAC 1 cut(s) 514
NspI RCATGY 3 cut(s) 687, 778, 857
PaeI GCATGC 1 cut(s) 778
PciI ACATGT 2 cut(s) 683, 853
PkrI GCNGC 3 cut(s) 1067, 1094, 1188
Ple19I CGATCG 1 cut(s) 152
PscI ACATGT 2 cut(s) 683, 853
PspN4I GGNNCC 1 cut(s) 1208
PspPI GGNCC 1 cut(s) 1206
PsuI RGATCY 1 cut(s) 320
PvuI CGATCG 1 cut(s) 152
RsaI GTAC 2 cut(s) 312, 817
RsaNI GTAC 2 cut(s) 311, 816
RseI CAYNNNNRTG 2 cut(s) 35, 231
SaqAI TTAA 6 cut(s) 423, 509, 737, 759, 858, 1172
SatI GCNGC 3 cut(s) 1066, 1093, 1187
Sau3AI GATC 4 cut(s) 120, 149, 153, 320
Sau96I GGNCC 1 cut(s) 1206
ScaI AGTACT 1 cut(s) 817
SduI GDGCHC 1 cut(s) 28
SetI ASST 8 cut(s) 136, 147, 169, 174, 274, 388, 588, 1107
SfaNI GCATC 1 cut(s) 1198
SmiMI CAYNNNNRTG 2 cut(s) 35, 231
SpeI ACTAGT 1 cut(s) 398
SphI GCATGC 1 cut(s) 778
SsiI CCGC 1 cut(s) 1090
SspI AATATT 1 cut(s) 632
SspMI CTAG 7 cut(s) 399, 573, 771, 783, 819, 839, 905
StyI CCWWGG 1 cut(s) 157
TaaI ACNGT 1 cut(s) 332
TaqI TCGA 2 cut(s) 152, 390
TatI WGTACW 2 cut(s) 310, 815
Tru1I TTAA 6 cut(s) 423, 509, 737, 759, 858, 1172
Tru9I TTAA 6 cut(s) 423, 509, 737, 759, 858, 1172
TscAI CASTG 1 cut(s) 1067
TseFI GTSAC 1 cut(s) 514
TseI GCWGC 3 cut(s) 1065, 1092, 1186
Tsp45I GTSAC 1 cut(s) 514
TspGWI ACGGA 1 cut(s) 636
TspRI CASTG 1 cut(s) 1067
XapI RAATTY 4 cut(s) 701, 885, 900, 1015
XbaI TCTAGA 2 cut(s) 782, 904
XceI RCATGY 3 cut(s) 687, 778, 857
XspI CTAG 7 cut(s) 399, 573, 771, 783, 819, 839, 905
ZrmI AGTACT 1 cut(s) 817
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.