pycom13g24270

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0019313
Physical Location & Seq
Forward (+)
21468107 .. 21472716
4610 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g24270.28

Sequence Viewer

Length: 3261 bp
ATGCAAAATATATTGCCATATAGAAGCTTACAACATTGTTGTTTGGATGGGAAAGACACTAATGGGGTGATAGCTCAAGCCAGGGAATCAAGGTCTCATCAAGGTCCGAACTCAAATGAGAGACTGAACCACATGATTGAGAATCATGCCTACTACTCAGCCATTTATGAAATGACTACAGAAGAGGTATCATCACTGATGACTAAGCTGATTGGCTCTAGTGCAAGTGGGAGATTGTTGGAAATGTGCCCTAAAACCAATCATATGATGATACTTTATGGACATTTCACATGCTTAAACGATAAGTCCAAGGAATATGTGATTGGAAGAATGCGATCTAAAGAAGTTAGATTCATGAGACCATTCTTTCATCAGTACGTGCTATGTCTTCTCTCAGGGAGAGTGACTAGTCTCGAGTCATTGGTGTGTGTGACATCAAGACAAGCATGTAGGTGCTCAATAGAGAATGAGTTCACTGAACACGATCAACGAGGGGTTCTCATATTCATGTCACATGAGAACACATGCTATCTTGTGGTTAGGTCCTTAATCTTTGATTATGTCAAAGTCACTCCATCAGGAGGGTGTCCACGGCGCAACAAGGGATCTCTAACCTTCAAACTGTTTGAGGGAGAATACTCTATGATATGTTTTAGAATCTCTGGCCAGAGTATGAATGAGATGTTGGAATGCGTTCCAAATCACATTCAAGGTAATCATATAAGCACAAGACTCGCATTGGATAAGTATTGTATTAGAGAAAGACCGTATTGCATTTGTAATCCTAAACTGAATAGGTTCTCCACCTCTTCTGATTACCCTAAAAGTGTATTATCACTAACGGGAGAATTGAAAGTAAGTTTCAATTCACAATCGATTAGAAAGAGTTTTAATCGCCCACTGCCTCTAAATGGTTTAATTATTTATCTAAGGCTAGGATTAATTAATATGTTAATTAATCAAACGAATAAGTTCGTTAAAGACCTCGGGATAGTTTTGGACCTTAAGGCCCAATGGGCTTCGAACGTCAATCACAAAGGCCAAAATAGCCCAATAAATCCCCTTAAATTCGGCCATTTAGAGGAGGGTAGTGAACTTGGCTTAATTAGCCATTTCATCCTAAGGGTTTTTCTAAGGAAAAAAGATGAGAACAAACTCTCCTTTCTCTCTAAAATGGTCACTCATTTCTTCTCCAATCTTACCTTGGTGTGGAGACTTAGAGGTTCTCAATTTTGGGAACTTGGAGAAACCTTTTCATCCATCCAAATCCATGCATCTAAGATGCAAGGAATGAAGGCCCTCTCTTTGGTTGAGTCTTGGTTCACCTATCTACTAGGCTTTGAAGTTCATGGGTTAATGCATGTTGTTGATGTTGCTCAAATGAACTTGTTTTTCACAAATATTCCTTCAGATGAACAACAAGTGGATGAGTTTCTCACAAAGGGTTTTATTCGGGAAAGCTTGTGTGGTTCCTGCCTTAGTCGAGCGATCAACAAAATCACAATTAAGTATCGATTTTCGATGCCAAGATTTAATGATCTTCTTTACCAACTGTGTGGTGCCATCGTACTCAGTCAAATTGATTTGAAAAGTGGGTATCATCAGATACGCATTAGAGAGGATGAATCGTTGGTCATGCCATTCGGGTTGACTAATGCTCCGAGTACTTTTATGCGAGTTATGAATCAGGTACTTCAGCCTTTTATTGGAACTTATGTGGTGGTATATTTTGATGACATTCTTGTCTACAGCACATGTCATGCCTTACATAAATTTTATGCAAATTTAAAGAAGTGTACATATATGACTGATCACATGCTGTTCCTTCGATACGTTGTATCTAAAAATGGAATACCTATGGATAATAAACAGGTTGAAGCAGTCACCACATGGCCTACGCCACAATCCATACATGATGTACAAAGTTTTCATGGATTTTATGGCGCTTTGATCACCGATTGTATGAATGGTGGAAAGTTCTCTTGGAATGGAGAAGCAGCAGTTGCCTTCAATCAAATCAAGAAAAAGTTGAGTTTGGCTTCGTTGCTTGTGTTACCTGACTTCTCAAAAGTTTTTGAGCTATCTTGCGATGCATCCAAGATTAGGATTGGAGCTATTCTAAGTCAAGAAGGGAAGCTTATTGCTTATTTCAATGAAAAACTGAGTAGTTCAAGATTGAATTATTCCACATATGACTTAGAATTCAATGCTATTTTTGTTATGTTCTCTGATCATGACTCTCTAAAGCATATAAACAGTCAGGAAAAATTGTCAACGAGGCATGCTAAGTGGGCAGCATACTTACAAGAATTTATATTTGTCTTAAAGCACAAAGTCAGTGTGCAAAATCAAGTTGCAGATGCCTTAAGTCGAAGGAGATATTTACTTGGTTCGATTAGAATTCAAGTATTAGGGTTTGACTCTTCGAAGGATTTATTGAAAATTGATCCTTTCTTCTCTACAATTGTCGAAAATGTTGAGGATGGAACTAGAACATATTATGTTTTGCATGAAGGGTTTCTCTTTAAAGGAAATCAACTTTTTACTAATGAAGGCCACGTTGGAAGGGACAAGACCTTTGAGCTTGTTTCTTTCTCTTATATTTGGCCTACCATGCGGAAAGAAGTATATCATTTTGTGGAACGATGCCGAATCTATCAAGTTTCCAAGGGCAAGGCGACCAACACTGGATTGTACATGTCATTACCTATTCCATCACAACCATGGGTGGATATAAGCATGGATTCTGTTTTGGATTTTGATAGGATTAAAAGAACACCACAAAGTAGCACACAAATAAAAATAAGGATCTATCCCGTAGAAGATAGTTTTATCCAACTACTTAAAATGTCACAAAAACTGGGTTACTACTTATCTAAGTCTATGAAATTTTATATGAAAACACTAGACACACAGAGCTACACTCACACAAATTTTTGGGACTTTTGTAAAAAACGAGTTAGGGGAACTGTTATCCACCAACGAATAATCATGCAAACATGTTTTGTTTCATTCAAATTCCTTTTATTTCCGGATGAAGATGCTCAAGTTGCCTTAATGTTAGAACTCAACCTATTACTCTTTCTTATGTACTGGAATGGCGTGGTCATAGATTTAACAAGCATCGTTAGTACTAGCGTTGTCTTACTTATCCTAGAAATTGATTCACATGTTAATATCGACAAGGGCAAGCACACACATATTCATAGCATTAGAATCCTAGATATGCTTACTAAGTATGCATCTGTAGAAAACACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 Retrotrans_gag rve zf-CCHC zf-H2C2

Protein Analysis

1087

Amino Acids

125.05

Weight (kDa)

8.99

Isoelectric Point (pI)

36.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1742
AccB1I GGYRCC 1 cut(s) 1559
AccI GTMKAC 1 cut(s) 1746
AccIII TCCGGA 1 cut(s) 3031
AciI CCGC 1 cut(s) 2617
AclWI GGATC 3 cut(s) 613, 2441, 2816
AcoI YGGCCR 2 cut(s) 664, 1072
AcsI RAATTY 9 cut(s) 1067, 1772, 1783, 2201, 2309, 2400, 2888, 2932, 3017
AcuI CTGAAG 2 cut(s) 1392, 1679
AfaI GTAC 9 cut(s) 377, 1569, 1666, 1692, 1798, 1920, 2696, 3092, 3133
AfiI CCNNNNNNNGG 7 cut(s) 581, 911, 1081, 1209, 1306, 1706, 2103
AflII CTTAAG 2 cut(s) 1004, 2365
AflIII ACRYGT 4 cut(s) 1754, 2697, 2999, 3169
AhlI ACTAGT 1 cut(s) 407
AjnI CCWGG 1 cut(s) 80
AjuI GAANNNNNNNTTGG 8 cut(s) 306, 338, 668, 700, 1966, 1998, 2544, 2576
AloI GAACNNNNNNTCC 2 cut(s) 1142, 1174
AluBI AGCT 9 cut(s) 27, 74, 208, 1461, 2080, 2114, 2137, 2584, 2919
AluI AGCT 9 cut(s) 27, 74, 208, 1461, 2080, 2114, 2137, 2584, 2919
Alw21I GWGCWC 1 cut(s) 458
Alw26I GTCTC 5 cut(s) 99, 115, 352, 416, 1207
AlwI GGATC 3 cut(s) 613, 2441, 2816
Ama87I CYCGRG 2 cut(s) 413, 986
Aor13HI TCCGGA 1 cut(s) 3031
AoxI GGCC 8 cut(s) 664, 1008, 1039, 1072, 1296, 1892, 2554, 2606
ApeKI GCWGC 2 cut(s) 1997, 2294
ApoI RAATTY 9 cut(s) 1067, 1772, 1783, 2201, 2309, 2400, 2888, 2932, 3017
ArsI GACNNNNNNTTYG 2 cut(s) 2561, 2593
AseI ATTAAT 3 cut(s) 941, 945, 957
Asp700I GAANNNNTTC 5 cut(s) 470, 693, 797, 971, 2517
AspLEI GCGC 2 cut(s) 597, 1946
AspS9I GGNCC 5 cut(s) 104, 543, 1000, 1009, 1297
AsuHPI GGTGA 4 cut(s) 79, 1315, 1876, 1945
AsuII TTCGAA 2 cut(s) 1022, 2426
AvaI CYCGRG 2 cut(s) 413, 986
AvaII GGWCC 3 cut(s) 104, 543, 1000
AxyI CCTNAGG 1 cut(s) 1121
BaeGI GKGCMC 1 cut(s) 251
BalI TGGCCA 1 cut(s) 666
BanI GGYRCC 1 cut(s) 1559
BbsI GAAGAC 1 cut(s) 380
Bbv12I GWGCWC 1 cut(s) 458
BbvI GCAGC 2 cut(s) 2009, 2306
BccI CCATC 6 cut(s) 41, 583, 1268, 1571, 2477, 2722
BceAI ACGGC 1 cut(s) 608
BcgI CGANNNNNNTGC 2 cut(s) 715, 749
BciT130I CCWGG 1 cut(s) 82
BclI TGATCA 3 cut(s) 1810, 1950, 2230
BcoDI GTCTC 5 cut(s) 99, 115, 352, 416, 1207
BcuI ACTAGT 1 cut(s) 407
BfaI CTAG 9 cut(s) 219, 408, 935, 1334, 2490, 2906, 3135, 3155, 3221
BfmI CTRYAG 3 cut(s) 177, 1747, 3246
BfoI RGCGCY 1 cut(s) 1947
BfrI CTTAAG 2 cut(s) 1004, 2365
BglI GCCNNNNNGGC 1 cut(s) 1016
BisI GCNGC 2 cut(s) 1998, 2295
BlsI GCNGC 2 cut(s) 1999, 2296
BmcAI AGTACT 2 cut(s) 1666, 3133
Bme1390I CCNGG 1 cut(s) 82
Bme18I GGWCC 3 cut(s) 104, 543, 1000
BmeT110I CYCGRG 2 cut(s) 413, 986
BmgT120I GGNCC 5 cut(s) 104, 543, 1000, 1009, 1297
BmiI GGNNCC 2 cut(s) 1471, 1561
BmrFI CCNGG 1 cut(s) 82
BmrI ACTGGG 1 cut(s) 2870
BmuI ACTGGG 1 cut(s) 2870
BpiI GAAGAC 1 cut(s) 380
BplI GAGNNNNNCTC 4 cut(s) 483, 515, 2908, 2940
Bpu14I TTCGAA 2 cut(s) 1022, 2426
BpuEI CTTGAG 2 cut(s) 60, 3030
Bsa29I ATCGAT 2 cut(s) 875, 1513
BsaAI YACGTR 1 cut(s) 379
BsaI GGTCTC 2 cut(s) 99, 352
BsaJI CCNNGG 7 cut(s) 81, 309, 590, 985, 1203, 2667, 2723
BsaWI WCCGGW 1 cut(s) 3031
BsaXI ACNNNNNCTCC 4 cut(s) 1142, 1172, 1642, 1672
Bsc4I CCNNNNNNNGG 7 cut(s) 581, 911, 1081, 1209, 1306, 1706, 2103
Bse1I ACTGG 3 cut(s) 2692, 2865, 3098
Bse21I CCTNAGG 1 cut(s) 1121
BseAI TCCGGA 1 cut(s) 3031
BseBI CCWGG 1 cut(s) 82
BseCI ATCGAT 2 cut(s) 875, 1513
BseDI CCNNGG 7 cut(s) 81, 309, 590, 985, 1203, 2667, 2723
BseGI GGATG 9 cut(s) 52, 1116, 1256, 1260, 1432, 1627, 2093, 2488, 3040
BseLI CCNNNNNNNGG 7 cut(s) 581, 911, 1081, 1209, 1306, 1706, 2103
BseMII CTCAG 4 cut(s) 171, 408, 1585, 2153
BseNI ACTGG 3 cut(s) 2692, 2865, 3098
BseRI GAGGAG 1 cut(s) 1097
BseSI GKGCMC 1 cut(s) 251
BseXI GCAGC 2 cut(s) 2009, 2306
BshFI GGCC 8 cut(s) 666, 1010, 1041, 1074, 1298, 1894, 2556, 2608
BshNI GGYRCC 1 cut(s) 1559
BshVI ATCGAT 2 cut(s) 875, 1513
BsiHKAI GWGCWC 1 cut(s) 458
BsiHKCI CYCGRG 2 cut(s) 413, 986
BsiSI CCGG 1 cut(s) 3032
BslFI GGGAC 2 cut(s) 2582, 2954
BslI CCNNNNNNNGG 7 cut(s) 581, 911, 1081, 1209, 1306, 1706, 2103
BsmAI GTCTC 5 cut(s) 99, 115, 352, 416, 1207
BsmFI GGGAC 2 cut(s) 2582, 2954
BsmI GAATGC 2 cut(s) 336, 695
BsnI GGCC 8 cut(s) 666, 1010, 1041, 1074, 1298, 1894, 2556, 2608
Bso31I GGTCTC 2 cut(s) 99, 352
BsoBI CYCGRG 2 cut(s) 413, 986
Bsp119I TTCGAA 2 cut(s) 1022, 2426
Bsp1286I GDGCHC 2 cut(s) 251, 458
Bsp13I TCCGGA 1 cut(s) 3031
Bsp1407I TGTACA 3 cut(s) 1796, 1918, 2694
Bsp19I CCATGG 1 cut(s) 2723
BspACI CCGC 1 cut(s) 2617
BspANI GGCC 8 cut(s) 666, 1010, 1041, 1074, 1298, 1894, 2556, 2608
BspCNI CTCAG 4 cut(s) 170, 407, 1584, 2154
BspDI ATCGAT 2 cut(s) 875, 1513
BspEI TCCGGA 1 cut(s) 3031
BspHI TCATGA 2 cut(s) 354, 2233
BspLI GGNNCC 2 cut(s) 1471, 1561
BspPI GGATC 3 cut(s) 613, 2441, 2816
BspT104I TTCGAA 2 cut(s) 1022, 2426
BspT107I GGYRCC 1 cut(s) 1559
BspTI CTTAAG 2 cut(s) 1004, 2365
BspTNI GGTCTC 2 cut(s) 99, 352
BsrGI TGTACA 3 cut(s) 1796, 1918, 2694
BsrI ACTGG 3 cut(s) 2692, 2865, 3098
BssECI CCNNGG 7 cut(s) 81, 309, 590, 985, 1203, 2667, 2723
BssT1I CCWWGG 4 cut(s) 309, 1203, 2667, 2723
Bst2UI CCWGG 1 cut(s) 82
Bst4CI ACNGT 5 cut(s) 624, 768, 1554, 2258, 2971
Bst6I CTCTTC 3 cut(s) 177, 814, 2428
BstAFI CTTAAG 2 cut(s) 1004, 2365
BstAPI GCANNNNNTGC 1 cut(s) 2003
BstAUI TGTACA 3 cut(s) 1796, 1918, 2694
BstBAI YACGTR 1 cut(s) 379
BstBI TTCGAA 2 cut(s) 1022, 2426
BstC8I GCNNGC 2 cut(s) 2283, 3191
BstDSI CCRYGG 2 cut(s) 590, 2723
BstF5I GGATG 9 cut(s) 52, 1116, 1256, 1260, 1432, 1627, 2093, 2488, 3040
BstH2I RGCGCY 1 cut(s) 1947
BstHHI GCGC 2 cut(s) 597, 1946
BstMAI GTCTC 5 cut(s) 99, 115, 352, 416, 1207
BstMWI GCNNNNNNNGC 7 cut(s) 1016, 1047, 1107, 2003, 2291, 2614, 3050
BstNI CCWGG 1 cut(s) 82
BstSCI CCNGG 1 cut(s) 80
BstSFI CTRYAG 3 cut(s) 177, 1747, 3246
BstSLI GKGCMC 1 cut(s) 251
BstV1I GCAGC 2 cut(s) 2009, 2306
BstV2I GAAGAC 1 cut(s) 380
BstX2I RGATCY 2 cut(s) 605, 2808
BstXI CCANNNNNNTGG 1 cut(s) 1556
BstYI RGATCY 2 cut(s) 605, 2808
Bsu15I ATCGAT 2 cut(s) 875, 1513
Bsu36I CCTNAGG 1 cut(s) 1121
BsuRI GGCC 8 cut(s) 666, 1010, 1041, 1074, 1298, 1894, 2556, 2608
BsuTUI ATCGAT 2 cut(s) 875, 1513
BtgI CCRYGG 2 cut(s) 590, 2723
BtgZI GCGATG 1 cut(s) 2103
BtsCI GGATG 9 cut(s) 52, 1116, 1256, 1260, 1432, 1627, 2093, 2488, 3040
BtsI GCAGTG 1 cut(s) 899
BtsIMutI CAGTG 5 cut(s) 194, 474, 899, 2344, 2685
Cac8I GCNNGC 2 cut(s) 2283, 3191
CciI TCATGA 2 cut(s) 354, 2233
CfoI GCGC 2 cut(s) 597, 1946
Cfr13I GGNCC 5 cut(s) 104, 543, 1000, 1009, 1297
ClaI ATCGAT 2 cut(s) 875, 1513
Csp6I GTAC 9 cut(s) 376, 1568, 1665, 1691, 1797, 1919, 2695, 3091, 3132
CviQI GTAC 9 cut(s) 376, 1568, 1665, 1691, 1797, 1919, 2695, 3091, 3132
DraI TTTAAA 2 cut(s) 1788, 2527
DrdI GACNNNNNNGTC 1 cut(s) 1742
DseDI GACNNNNNNGTC 1 cut(s) 1742
EaeI YGGCCR 2 cut(s) 664, 1072
Eam1104I CTCTTC 3 cut(s) 177, 814, 2428
EarI CTCTTC 3 cut(s) 177, 814, 2428
Eco130I CCWWGG 4 cut(s) 309, 1203, 2667, 2723
Eco31I GGTCTC 2 cut(s) 99, 352
Eco47I GGWCC 3 cut(s) 104, 543, 1000
Eco57I CTGAAG 2 cut(s) 1392, 1679
Eco81I CCTNAGG 1 cut(s) 1121
Eco88I CYCGRG 2 cut(s) 413, 986
EcoO109I RGGNCCY 2 cut(s) 543, 1297
EcoRI GAATTC 2 cut(s) 2201, 2400
EcoRII CCWGG 1 cut(s) 80
EcoT14I CCWWGG 4 cut(s) 309, 1203, 2667, 2723
EcoT22I ATGCAT 4 cut(s) 1276, 1362, 2095, 3244
ErhI CCWWGG 4 cut(s) 309, 1203, 2667, 2723
FalI AAGNNNNNCTT 2 cut(s) 2121, 2153
FaqI GGGAC 2 cut(s) 2582, 2954
FauNDI CATATG 2 cut(s) 264, 2191
FbaI TGATCA 3 cut(s) 1810, 1950, 2230
FblI GTMKAC 1 cut(s) 1746
Fnu4HI GCNGC 2 cut(s) 1998, 2295
FokI GGATG 9 cut(s) 59, 1103, 1243, 1247, 1439, 1634, 2080, 2495, 3047
Fsp4HI GCNGC 2 cut(s) 1998, 2295
FspBI CTAG 9 cut(s) 219, 408, 935, 1334, 2490, 2906, 3135, 3155, 3221
GlaI GCGC 2 cut(s) 596, 1945
GluI GCNGC 2 cut(s) 1998, 2295
HaeII RGCGCY 1 cut(s) 1947
HaeIII GGCC 8 cut(s) 666, 1010, 1041, 1074, 1298, 1894, 2556, 2608
HapII CCGG 1 cut(s) 3032
HhaI GCGC 2 cut(s) 597, 1946
Hin6I GCGC 2 cut(s) 595, 1944
HinP1I GCGC 2 cut(s) 595, 1944
HincII GTYRAC 2 cut(s) 1650, 2274
HindII GTYRAC 2 cut(s) 1650, 2274
HindIII AAGCTT 3 cut(s) 25, 1459, 2135
HpaII CCGG 1 cut(s) 3032
HphI GGTGA 4 cut(s) 79, 1315, 1876, 1945
Hpy166II GTNNAC 8 cut(s) 474, 590, 1094, 1323, 1650, 1747, 1797, 2274
Hpy188I TCNGA 6 cut(s) 108, 814, 1411, 1605, 1662, 2230
Hpy8I GTNNAC 8 cut(s) 474, 590, 1094, 1323, 1650, 1747, 1797, 2274
HpyCH4III ACNGT 5 cut(s) 624, 768, 1554, 2258, 2971
HpyCH4IV ACGT 4 cut(s) 378, 1026, 1833, 2559
HpyF10VI GCNNNNNNNGC 7 cut(s) 1016, 1047, 1107, 2003, 2291, 2614, 3050
HpySE526I ACGT 4 cut(s) 378, 1026, 1833, 2559
HspAI GCGC 2 cut(s) 595, 1944
Kpn2I TCCGGA 1 cut(s) 3031
Ksp22I TGATCA 3 cut(s) 1810, 1950, 2230
LmnI GCTCC 2 cut(s) 1663, 2111
Lsp1109I GCAGC 2 cut(s) 2009, 2306
MaeI CTAG 9 cut(s) 219, 408, 935, 1334, 2490, 2906, 3135, 3155, 3221
MaeII ACGT 4 cut(s) 378, 1026, 1833, 2559
MaeIII GTNAC 9 cut(s) 403, 430, 510, 568, 1177, 1882, 2052, 2850, 2864
MfeI CAATTG 1 cut(s) 2463
MflI RGATCY 2 cut(s) 605, 2808
MhlI GDGCHC 2 cut(s) 251, 458
MlsI TGGCCA 1 cut(s) 666
MluNI TGGCCA 1 cut(s) 666
MlyI GAGTC 5 cut(s) 425, 726, 1322, 2231, 2414
MmeI TCCRAC 4 cut(s) 219, 666, 2542, 2860
Mox20I TGGCCA 1 cut(s) 666
Mph1103I ATGCAT 4 cut(s) 1276, 1362, 2095, 3244
MroI TCCGGA 1 cut(s) 3031
MroXI GAANNNNTTC 5 cut(s) 470, 693, 797, 971, 2517
MscI TGGCCA 1 cut(s) 666
MslI CAYNNNNRTG 4 cut(s) 424, 451, 506, 2722
Msp20I TGGCCA 1 cut(s) 666
MspCI CTTAAG 2 cut(s) 1004, 2365
MspI CCGG 1 cut(s) 3032
MspR9I CCNGG 1 cut(s) 82
MunI CAATTG 1 cut(s) 2463
Mva1269I GAATGC 2 cut(s) 336, 695
MvaI CCWGG 1 cut(s) 82
MwoI GCNNNNNNNGC 7 cut(s) 1016, 1047, 1107, 2003, 2291, 2614, 3050
NcoI CCATGG 1 cut(s) 2723
NdeI CATATG 2 cut(s) 264, 2191
NlaIV GGNNCC 2 cut(s) 1471, 1561
NmuCI GTSAC 7 cut(s) 403, 430, 510, 568, 1177, 1882, 2850
NsiI ATGCAT 4 cut(s) 1276, 1362, 2095, 3244
NspV TTCGAA 2 cut(s) 1022, 2426
PacI TTAATTAA 2 cut(s) 945, 957
PaeI GCATGC 1 cut(s) 2285
PaeR7I CTCGAG 1 cut(s) 413
PagI TCATGA 2 cut(s) 354, 2233
PciI ACATGT 4 cut(s) 1754, 2697, 2999, 3169
PctI GAATGC 2 cut(s) 336, 695
PdmI GAANNNNTTC 5 cut(s) 470, 693, 797, 971, 2517
PkrI GCNGC 2 cut(s) 1999, 2296
PleI GAGTC 5 cut(s) 424, 726, 1321, 2231, 2414
PpsI GAGTC 5 cut(s) 424, 726, 1321, 2231, 2414
Ppu21I YACGTR 1 cut(s) 379
PpuMI RGGWCCY 1 cut(s) 543
PscI ACATGT 4 cut(s) 1754, 2697, 2999, 3169
PshBI ATTAAT 3 cut(s) 941, 945, 957
Psp5II RGGWCCY 1 cut(s) 543
Psp6I CCWGG 1 cut(s) 80
PspGI CCWGG 1 cut(s) 80
PspN4I GGNNCC 2 cut(s) 1471, 1561
PspPI GGNCC 5 cut(s) 104, 543, 1000, 1009, 1297
PspPPI RGGWCCY 1 cut(s) 543
PsuI RGATCY 2 cut(s) 605, 2808
RsaI GTAC 9 cut(s) 377, 1569, 1666, 1692, 1798, 1920, 2696, 3092, 3133
RsaNI GTAC 9 cut(s) 376, 1568, 1665, 1691, 1797, 1919, 2695, 3091, 3132
RseI CAYNNNNRTG 4 cut(s) 424, 451, 506, 2722
SatI GCNGC 2 cut(s) 1998, 2295
Sau96I GGNCC 5 cut(s) 104, 543, 1000, 1009, 1297
ScaI AGTACT 2 cut(s) 1666, 3133
SchI GAGTC 5 cut(s) 425, 726, 1322, 2231, 2414
ScrFI CCNGG 1 cut(s) 82
SduI GDGCHC 2 cut(s) 251, 458
SfcI CTRYAG 3 cut(s) 177, 1747, 3246
Sfr274I CTCGAG 1 cut(s) 413
SfuI TTCGAA 2 cut(s) 1022, 2426
SinI GGWCC 3 cut(s) 104, 543, 1000
SlaI CTCGAG 1 cut(s) 413
SmiMI CAYNNNNRTG 4 cut(s) 424, 451, 506, 2722
SmlI CTYRAG 5 cut(s) 75, 413, 1004, 2365, 3045
SmoI CTYRAG 5 cut(s) 75, 413, 1004, 2365, 3045
SpeI ACTAGT 1 cut(s) 407
SphI GCATGC 1 cut(s) 2285
SsiI CCGC 1 cut(s) 2617
SspI AATATT 1 cut(s) 1402
SspMI CTAG 9 cut(s) 219, 408, 935, 1334, 2490, 2906, 3135, 3155, 3221
StyD4I CCNGG 1 cut(s) 80
StyI CCWWGG 4 cut(s) 309, 1203, 2667, 2723
TaaI ACNGT 5 cut(s) 624, 768, 1554, 2258, 2971
TaiI ACGT 4 cut(s) 381, 1029, 1836, 2562
TatI WGTACW 6 cut(s) 1664, 1796, 1918, 2694, 3090, 3131
TscAI CASTG 5 cut(s) 201, 481, 906, 2344, 2692
TseFI GTSAC 7 cut(s) 403, 430, 510, 568, 1177, 1882, 2850
TseI GCWGC 2 cut(s) 1997, 2294
Tsp45I GTSAC 7 cut(s) 403, 430, 510, 568, 1177, 1882, 2850
TspRI CASTG 5 cut(s) 201, 481, 906, 2344, 2692
Vha464I CTTAAG 2 cut(s) 1004, 2365
VpaK11BI GGWCC 3 cut(s) 104, 543, 1000
VspI ATTAAT 3 cut(s) 941, 945, 957
XapI RAATTY 9 cut(s) 1067, 1772, 1783, 2201, 2309, 2400, 2888, 2932, 3017
XcmI CCANNNNNNNNNTGG 2 cut(s) 1201, 2721
XhoI CTCGAG 1 cut(s) 413
XmiI GTMKAC 1 cut(s) 1746
XmnI GAANNNNTTC 5 cut(s) 470, 693, 797, 971, 2517
XspI CTAG 9 cut(s) 219, 408, 935, 1334, 2490, 2906, 3135, 3155, 3221
ZrmI AGTACT 2 cut(s) 1666, 3133
Zsp2I ATGCAT 4 cut(s) 1276, 1362, 2095, 3244
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.