pycom01g13240

DNL-type zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0015054
Physical Location & Seq
Reverse (-)
13754031 .. 13756057
2027 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 582 bp
ATGGCGGCTCGAATGTTGCAGAGACGCTTAGGCTCACTCCTCCTCCGCAATCAGCAGCCTAACGCCCATCTCTTCAAAGATCATTTTGTTCCTTCTGCAAGTTCCATACTCAGAAGATACGGATTTGACAAAAGAGAGATTCAGACGCAAACAAATCCAACAAATCCGGCTAATGAGGGTTTGGTGAATCTTGAAGATAGCCGTTTGAAGCCTAAGATTGACGCAAATGCTATCAACAGTTCCGATGCCAAATTTGCTGATGCTTCCACCCAGAAAATATCCCCAAGGCATGACCTTGCCATGCTTTTTACTTGCAAAGTCTGTGAAACAAGATCTATGAAGACGTGCAGCAGAGAATCATACGAGAAAGGTGTGGTGGTAGCTCGCTGCGGCGGGTGTAACAATCTCCACCTGATTGCTGATCATCTCGGGTATTTCGGACAACCATCCAGCATTGAGCAATTTCTGGCTGCCCGCGGGGAAGAGGTGAAGAAAGGTTCCGCCGATACGCTGAATCTGACTCTTGAAGATATAGCCGGAAAGAAACCTGGTGAAGGGGTTGAAGTTAAGCAATTGGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-DNL

Protein Analysis

194

Amino Acids

21.33

Weight (kDa)

8.81

Isoelectric Point (pI)

38.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-DNL PF05180 101 - 161 1.6e-24 DNL zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015054)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G54826
fragaria_vesca FvH4_7g19000
malus_domestica MD01G1105600.v1.1 MD07G1171800.v1.1
pyrus_communis pycom01g13240 pycom07g16640
rosa_chinensis RchiOBHm_Chr1g0362931
rosa_laevigata RLG00000027611
rosa_multiflora Rmu_sc0001502.1_g000005
rosa_roxburghii Rroxscaffold_4G00293640
rosa_rugosa Rorug01G0302200
rosa_samantha Rh1AG311700 Rh1BG274800 Rh1CG292000 Rh1DG305500
rosa_wichuraiana Rw1G027620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 477
AciI CCGC 7 cut(s) 5, 46, 390, 393, 475, 477, 501
AcsI RAATTY 1 cut(s) 251
AfiI CCNNNNNNNGG 1 cut(s) 554
AgsI TTSAA 5 cut(s) 76, 194, 208, 527, 563
AjiI CACGTC 1 cut(s) 345
AjnI CCWGG 1 cut(s) 547
AluBI AGCT 1 cut(s) 383
AluI AGCT 1 cut(s) 383
Alw26I GTCTC 1 cut(s) 16
Ama87I CYCGRG 1 cut(s) 428
ApeKI GCWGC 4 cut(s) 55, 348, 387, 470
ApoI RAATTY 1 cut(s) 251
AsuHPI GGTGA 3 cut(s) 196, 499, 563
AvaI CYCGRG 1 cut(s) 428
BbsI GAAGAC 1 cut(s) 347
BbvI GCAGC 4 cut(s) 67, 360, 374, 457
BccI CCATC 2 cut(s) 75, 454
BceAI ACGGC 1 cut(s) 186
BciT130I CCWGG 1 cut(s) 549
BclI TGATCA 1 cut(s) 421
BcoDI GTCTC 1 cut(s) 16
BglII AGATCT 1 cut(s) 332
BisI GCNGC 6 cut(s) 6, 56, 349, 388, 391, 471
BlsI GCNGC 6 cut(s) 7, 57, 350, 389, 392, 472
Bme1390I CCNGG 1 cut(s) 549
BmeT110I CYCGRG 1 cut(s) 428
BmgBI CACGTC 1 cut(s) 345
BmiI GGNNCC 1 cut(s) 499
BmrFI CCNGG 1 cut(s) 549
BmsI GCATC 2 cut(s) 235, 250
BpiI GAAGAC 1 cut(s) 347
Bpu10I CCTNAGC 1 cut(s) 28
BsaJI CCNNGG 2 cut(s) 284, 475
BsaXI ACNNNNNCTCC 2 cut(s) 27, 57
Bsc4I CCNNNNNNNGG 1 cut(s) 554
BseBI CCWGG 1 cut(s) 549
BseDI CCNNGG 2 cut(s) 284, 475
BseGI GGATG 1 cut(s) 446
BseLI CCNNNNNNNGG 1 cut(s) 554
BseMII CTCAG 1 cut(s) 124
BseRI GAGGAG 2 cut(s) 29, 32
BseXI GCAGC 4 cut(s) 67, 360, 374, 457
BsgI GTGCAG 1 cut(s) 367
Bsh1236I CGCG 1 cut(s) 477
BsiHKCI CYCGRG 1 cut(s) 428
BsiSI CCGG 2 cut(s) 167, 537
BslI CCNNNNNNNGG 1 cut(s) 554
BsmAI GTCTC 1 cut(s) 16
BsmBI CGTCTC 1 cut(s) 16
BsoBI CYCGRG 1 cut(s) 428
Bsp143I GATC 3 cut(s) 79, 332, 421
BspACI CCGC 7 cut(s) 5, 46, 390, 393, 475, 477, 501
BspCNI CTCAG 1 cut(s) 123
BspFNI CGCG 1 cut(s) 477
BspLI GGNNCC 1 cut(s) 499
BssECI CCNNGG 2 cut(s) 284, 475
BssMI GATC 3 cut(s) 79, 332, 421
BssT1I CCWWGG 1 cut(s) 284
Bst2UI CCWGG 1 cut(s) 549
Bst4CI ACNGT 1 cut(s) 239
Bst6I CTCTTC 2 cut(s) 77, 477
BstC8I GCNNGC 2 cut(s) 385, 475
BstDEI CTNAG 3 cut(s) 28, 110, 213
BstDSI CCRYGG 1 cut(s) 475
BstENI CCTNNNNNAGG 1 cut(s) 552
BstF5I GGATG 1 cut(s) 446
BstFNI CGCG 1 cut(s) 477
BstKTI GATC 3 cut(s) 82, 335, 424
BstMAI GTCTC 1 cut(s) 16
BstMBI GATC 3 cut(s) 79, 332, 421
BstMWI GCNNNNNNNGC 1 cut(s) 254
BstNI CCWGG 1 cut(s) 549
BstSCI CCNGG 1 cut(s) 547
BstUI CGCG 1 cut(s) 477
BstV1I GCAGC 4 cut(s) 67, 360, 374, 457
BstV2I GAAGAC 1 cut(s) 347
BstX2I RGATCY 1 cut(s) 332
BstYI RGATCY 1 cut(s) 332
BtgI CCRYGG 1 cut(s) 475
BtrI CACGTC 1 cut(s) 345
BtsCI GGATG 1 cut(s) 446
Cac8I GCNNGC 2 cut(s) 385, 475
Cfr42I CCGCGG 1 cut(s) 478
CseI GACGC 3 cut(s) 33, 154, 230
CsiI ACCWGGT 1 cut(s) 547
CviAII CATG 2 cut(s) 290, 301
CviJI RGCY 9 cut(s) 8, 33, 58, 170, 201, 211, 383, 470, 536
CviKI_1 RGCY 9 cut(s) 8, 33, 58, 170, 201, 211, 383, 470, 536
DdeI CTNAG 3 cut(s) 28, 110, 213
DpnI GATC 3 cut(s) 81, 334, 423
DpnII GATC 3 cut(s) 79, 332, 421
Eam1104I CTCTTC 2 cut(s) 77, 477
EarI CTCTTC 2 cut(s) 77, 477
EciI GGCGGA 1 cut(s) 490
Eco130I CCWWGG 1 cut(s) 284
Eco88I CYCGRG 1 cut(s) 428
EcoNI CCTNNNNNAGG 1 cut(s) 552
EcoRII CCWGG 1 cut(s) 547
EcoT14I CCWWGG 1 cut(s) 284
ErhI CCWWGG 1 cut(s) 284
Esp3I CGTCTC 1 cut(s) 16
FaeI CATG 2 cut(s) 293, 304
FaiI YATR 6 cut(s) 107, 291, 302, 338, 361, 533
FatI CATG 2 cut(s) 289, 300
FauI CCCGC 3 cut(s) 386, 470, 482
FbaI TGATCA 1 cut(s) 421
Fnu4HI GCNGC 6 cut(s) 6, 56, 349, 388, 391, 471
FokI GGATG 1 cut(s) 433
Fsp4HI GCNGC 6 cut(s) 6, 56, 349, 388, 391, 471
GluI GCNGC 6 cut(s) 6, 56, 349, 388, 391, 471
HapII CCGG 2 cut(s) 167, 537
HgaI GACGC 3 cut(s) 33, 154, 230
Hin1II CATG 2 cut(s) 293, 304
HinfI GANTC 5 cut(s) 139, 187, 356, 514, 520
HpaII CCGG 2 cut(s) 167, 537
HphI GGTGA 3 cut(s) 196, 499, 563
Hpy188I TCNGA 5 cut(s) 113, 144, 244, 440, 519
Hpy188III TCNNGA 2 cut(s) 191, 524
HpyAV CCTTC 2 cut(s) 102, 548
HpyCH4III ACNGT 1 cut(s) 239
HpyCH4IV ACGT 1 cut(s) 344
HpyCH4V TGCA 4 cut(s) 19, 98, 315, 348
HpyF10VI GCNNNNNNNGC 1 cut(s) 254
HpyF3I CTNAG 3 cut(s) 28, 110, 213
HpySE526I ACGT 1 cut(s) 344
Hsp92II CATG 2 cut(s) 293, 304
Ksp22I TGATCA 1 cut(s) 421
KspI CCGCGG 1 cut(s) 478
Kzo9I GATC 3 cut(s) 79, 332, 421
LpnPI CCDG 8 cut(s) 180, 284, 425, 452, 463, 534, 550, 561
Lsp1109I GCAGC 4 cut(s) 67, 360, 374, 457
LweI GCATC 2 cut(s) 235, 250
MabI ACCWGGT 1 cut(s) 547
MaeII ACGT 1 cut(s) 344
MaeIII GTNAC 1 cut(s) 398
MalI GATC 3 cut(s) 81, 334, 423
MboI GATC 3 cut(s) 79, 332, 421
MboII GAAGA 7 cut(s) 64, 126, 206, 352, 494, 502, 539
MfeI CAATTG 1 cut(s) 572
MflI RGATCY 1 cut(s) 332
MluCI AATT 3 cut(s) 251, 461, 572
MlyI GAGTC 1 cut(s) 514
MmeI TCCRAC 1 cut(s) 182
MnlI CCTC 4 cut(s) 50, 53, 169, 478
MseI TTAA 1 cut(s) 567
MspA1I CMGCKG 1 cut(s) 477
MspI CCGG 2 cut(s) 167, 537
MspR9I CCNGG 1 cut(s) 549
MunI CAATTG 1 cut(s) 572
MvaI CCWGG 1 cut(s) 549
MvnI CGCG 1 cut(s) 477
MwoI GCNNNNNNNGC 1 cut(s) 254
NdeII GATC 3 cut(s) 79, 332, 421
NlaIII CATG 2 cut(s) 293, 304
NlaIV GGNNCC 1 cut(s) 499
PfeI GAWTC 4 cut(s) 139, 187, 356, 514
PkrI GCNGC 6 cut(s) 7, 57, 350, 389, 392, 472
PleI GAGTC 1 cut(s) 514
PpsI GAGTC 1 cut(s) 514
Psp6I CCWGG 1 cut(s) 547
PspGI CCWGG 1 cut(s) 547
PspN4I GGNNCC 1 cut(s) 499
PsuI RGATCY 1 cut(s) 332
SacII CCGCGG 1 cut(s) 478
SaqAI TTAA 1 cut(s) 567
SatI GCNGC 6 cut(s) 6, 56, 349, 388, 391, 471
Sau3AI GATC 3 cut(s) 79, 332, 421
SchI GAGTC 1 cut(s) 514
ScrFI CCNGG 1 cut(s) 549
SetI ASST 8 cut(s) 297, 347, 373, 385, 414, 489, 499, 550
SexAI ACCWGGT 1 cut(s) 547
SfaNI GCATC 2 cut(s) 235, 250
Sfr303I CCGCGG 1 cut(s) 478
SgrBI CCGCGG 1 cut(s) 478
Sse9I AATT 3 cut(s) 251, 461, 572
SsiI CCGC 7 cut(s) 5, 46, 390, 393, 475, 477, 501
StyD4I CCNGG 1 cut(s) 547
StyI CCWWGG 1 cut(s) 284
TaaI ACNGT 1 cut(s) 239
TaiI ACGT 1 cut(s) 347
TaqI TCGA 1 cut(s) 10
TasI AATT 3 cut(s) 251, 461, 572
TauI GCSGC 2 cut(s) 8, 393
TfiI GAWTC 4 cut(s) 139, 187, 356, 514
Tru1I TTAA 1 cut(s) 567
Tru9I TTAA 1 cut(s) 567
TseI GCWGC 4 cut(s) 55, 348, 387, 470
TspDTI ATGAA 1 cut(s) 353
TspGWI ACGGA 1 cut(s) 135
XagI CCTNNNNNAGG 1 cut(s) 552
XapI RAATTY 1 cut(s) 251
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.