pycom01g16580

Polyketide cyclase / dehydrase and lipid transport

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0017716
Physical Location & Seq
Forward (+)
16097384 .. 16098075
692 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g16580.1

Sequence Viewer

Length: 492 bp
ATGGCATCTGAGGCCAGAACTGGCAAATGGCGCGGAACAGTAGGGGGCATTGTTGAAGCTCCTATAGACAAAGTATGGAGCATGGTTTCTCAAACCAAAAGACTAGCCGAATGGATGCCGATGGTTGAGCGCTGCACTGATCTAGCTGGAGAGGAAGGCGTTCCAGGATATGTTCGGTTAGTCTCGGGCTTCATGTTCCCTCAAGACGATGGAGACAGGTCATGGATCAAGGAGAAGTTGGTGTCCATGGACTCATCACAACATAGTTATGCGTACAAACTGGAAGCAAGCAACGTAGGGTTGGATGGGTCTGTAAATTCACTGAAGCTTGTAGATTATGGGGATGAGTCAACCCTAGTTGACTGGTCATTTGAGGTCAACCCCGTAGAAGGTGCATGTGAGGACAGCACTGTAGATTATCTAGGGTTTCTGTATAAGTCTTGCATTAAGAGGGTTGAAGCTGCTTTAGAAGCAGCATCCAAGAAAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Polyketide_cyc2

Protein Analysis

164

Amino Acids

17.99

Weight (kDa)

4.72

Isoelectric Point (pI)

33.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Polyketide_cyc2 PF10604 18 - 156 3.7e-14 Polyketide cyclase / dehydrase and lipid transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017716)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g23970
malus_domestica MD01G1146400.v1.1
pyrus_communis pycom01g16580
rosa_chinensis RchiOBHm_Chr1g0369311
rosa_laevigata RLG00000027138
rosa_roxburghii Rroxscaffold_4G00287740
rosa_rugosa Rorug01G0349500
rosa_samantha Rh1AG356700 Rh1BG320700 Rh1CG334700 Rh1DG351400
rosa_wichuraiana Rw1G031490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 33
AciI CCGC 1 cut(s) 33
AclWI GGATC 1 cut(s) 233
AcsI RAATTY 1 cut(s) 316
AcuI CTGAAG 1 cut(s) 344
AfaI GTAC 1 cut(s) 275
AfeI AGCGCT 1 cut(s) 131
AfiI CCNNNNNNNGG 1 cut(s) 389
AgsI TTSAA 2 cut(s) 56, 458
AjnI CCWGG 1 cut(s) 163
AluBI AGCT 4 cut(s) 59, 146, 328, 461
AluI AGCT 4 cut(s) 59, 146, 328, 461
Alw26I GTCTC 2 cut(s) 187, 207
AlwI GGATC 1 cut(s) 233
Ama87I CYCGRG 1 cut(s) 184
Aor51HI AGCGCT 1 cut(s) 131
AoxI GGCC 1 cut(s) 12
ApeKI GCWGC 3 cut(s) 132, 461, 473
ApoI RAATTY 1 cut(s) 316
ArsI GACNNNNNNTTYG 2 cut(s) 353, 385
Asp700I GAANNNNTTC 1 cut(s) 159
AspLEI GCGC 2 cut(s) 33, 132
AvaI CYCGRG 1 cut(s) 184
BbvI GCAGC 3 cut(s) 119, 448, 485
BccI CCATC 3 cut(s) 115, 203, 299
BciT130I CCWGG 1 cut(s) 165
BcoDI GTCTC 2 cut(s) 187, 207
BfaI CTAG 4 cut(s) 104, 143, 356, 422
BfmI CTRYAG 2 cut(s) 63, 411
BfoI RGCGCY 1 cut(s) 133
BisI GCNGC 3 cut(s) 133, 462, 474
BlsI GCNGC 3 cut(s) 134, 463, 475
Bme1390I CCNGG 1 cut(s) 165
BmeT110I CYCGRG 1 cut(s) 184
BmrFI CCNGG 1 cut(s) 165
BmsI GCATC 3 cut(s) 14, 105, 485
BpmI CTGGAG 1 cut(s) 168
BpuEI CTTGAG 1 cut(s) 186
BsaJI CCNNGG 1 cut(s) 246
Bsc4I CCNNNNNNNGG 1 cut(s) 389
Bse1I ACTGG 3 cut(s) 25, 285, 368
BseBI CCWGG 1 cut(s) 165
BseDI CCNNGG 1 cut(s) 246
BseGI GGATG 4 cut(s) 120, 310, 349, 476
BseLI CCNNNNNNNGG 1 cut(s) 389
BseNI ACTGG 3 cut(s) 25, 285, 368
BseXI GCAGC 3 cut(s) 119, 448, 485
BsgI GTGCAG 1 cut(s) 118
Bsh1236I CGCG 1 cut(s) 33
BshFI GGCC 1 cut(s) 14
BsiHKCI CYCGRG 1 cut(s) 184
BslI CCNNNNNNNGG 1 cut(s) 389
BsmAI GTCTC 2 cut(s) 187, 207
BsnI GGCC 1 cut(s) 14
BsoBI CYCGRG 1 cut(s) 184
Bsp143I GATC 2 cut(s) 139, 225
Bsp19I CCATGG 1 cut(s) 246
BspACI CCGC 1 cut(s) 33
BspANI GGCC 1 cut(s) 14
BspFNI CGCG 1 cut(s) 33
BspPI GGATC 1 cut(s) 233
BsrI ACTGG 3 cut(s) 25, 285, 368
BssECI CCNNGG 1 cut(s) 246
BssMI GATC 2 cut(s) 139, 225
BssT1I CCWWGG 1 cut(s) 246
Bst2UI CCWGG 1 cut(s) 165
Bst4CI ACNGT 2 cut(s) 40, 412
BstC8I GCNNGC 1 cut(s) 289
BstDEI CTNAG 1 cut(s) 9
BstDSI CCRYGG 1 cut(s) 246
BstF5I GGATG 4 cut(s) 120, 310, 349, 476
BstFNI CGCG 1 cut(s) 33
BstH2I RGCGCY 1 cut(s) 133
BstHHI GCGC 2 cut(s) 33, 132
BstKTI GATC 2 cut(s) 142, 228
BstMAI GTCTC 2 cut(s) 187, 207
BstMBI GATC 2 cut(s) 139, 225
BstMWI GCNNNNNNNGC 3 cut(s) 11, 30, 470
BstNI CCWGG 1 cut(s) 165
BstNSI RCATGY 1 cut(s) 399
BstSCI CCNGG 1 cut(s) 163
BstSFI CTRYAG 2 cut(s) 63, 411
BstUI CGCG 1 cut(s) 33
BstV1I GCAGC 3 cut(s) 119, 448, 485
BsuRI GGCC 1 cut(s) 14
BtgI CCRYGG 1 cut(s) 246
BtsCI GGATG 4 cut(s) 120, 310, 349, 476
BtsIMutI CAGTG 3 cut(s) 135, 320, 408
Cac8I GCNNGC 1 cut(s) 289
CfoI GCGC 2 cut(s) 33, 132
Csp6I GTAC 1 cut(s) 274
CviAII CATG 5 cut(s) 82, 193, 222, 247, 396
CviJI RGCY 7 cut(s) 14, 59, 107, 146, 189, 328, 461
CviKI_1 RGCY 7 cut(s) 14, 59, 107, 146, 189, 328, 461
CviQI GTAC 1 cut(s) 274
DdeI CTNAG 1 cut(s) 9
DpnI GATC 2 cut(s) 141, 227
DpnII GATC 2 cut(s) 139, 225
Eco130I CCWWGG 1 cut(s) 246
Eco47III AGCGCT 1 cut(s) 131
Eco57I CTGAAG 1 cut(s) 344
Eco88I CYCGRG 1 cut(s) 184
EcoRII CCWGG 1 cut(s) 163
EcoT14I CCWWGG 1 cut(s) 246
ErhI CCWWGG 1 cut(s) 246
FaeI CATG 5 cut(s) 85, 196, 225, 250, 399
FatI CATG 5 cut(s) 81, 192, 221, 246, 395
Fnu4HI GCNGC 3 cut(s) 133, 462, 474
FokI GGATG 4 cut(s) 127, 317, 356, 463
Fsp4HI GCNGC 3 cut(s) 133, 462, 474
FspBI CTAG 4 cut(s) 104, 143, 356, 422
GlaI GCGC 2 cut(s) 32, 131
GluI GCNGC 3 cut(s) 133, 462, 474
GsuI CTGGAG 1 cut(s) 168
HaeII RGCGCY 1 cut(s) 133
HaeIII GGCC 1 cut(s) 14
HhaI GCGC 2 cut(s) 33, 132
Hin1II CATG 5 cut(s) 85, 196, 225, 250, 399
Hin6I GCGC 2 cut(s) 31, 130
HinP1I GCGC 2 cut(s) 31, 130
HincII GTYRAC 3 cut(s) 351, 361, 379
HindII GTYRAC 3 cut(s) 351, 361, 379
HindIII AAGCTT 1 cut(s) 326
HinfI GANTC 2 cut(s) 251, 347
Hpy166II GTNNAC 3 cut(s) 351, 361, 379
Hpy188I TCNGA 1 cut(s) 10
Hpy188III TCNNGA 1 cut(s) 203
Hpy8I GTNNAC 3 cut(s) 351, 361, 379
HpyAV CCTTC 2 cut(s) 149, 383
HpyCH4III ACNGT 2 cut(s) 40, 412
HpyCH4IV ACGT 1 cut(s) 294
HpyCH4V TGCA 3 cut(s) 135, 395, 444
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 30, 470
HpyF3I CTNAG 1 cut(s) 9
HpySE526I ACGT 1 cut(s) 294
Hsp92II CATG 5 cut(s) 85, 196, 225, 250, 399
HspAI GCGC 2 cut(s) 31, 130
Kzo9I GATC 2 cut(s) 139, 225
LmnI GCTCC 2 cut(s) 64, 78
LpnPI CCDG 8 cut(s) 6, 28, 132, 150, 177, 202, 266, 349
Lsp1109I GCAGC 3 cut(s) 119, 448, 485
LweI GCATC 3 cut(s) 14, 105, 485
MaeI CTAG 4 cut(s) 104, 143, 356, 422
MaeII ACGT 1 cut(s) 294
MalI GATC 2 cut(s) 141, 227
MboI GATC 2 cut(s) 139, 225
MluCI AATT 1 cut(s) 316
MlyI GAGTC 2 cut(s) 245, 356
MmeI TCCRAC 1 cut(s) 282
MnlI CCTC 6 cut(s) 4, 145, 210, 367, 394, 444
MroXI GAANNNNTTC 1 cut(s) 159
MseI TTAA 1 cut(s) 447
MslI CAYNNNNRTG 1 cut(s) 267
MspR9I CCNGG 1 cut(s) 165
MvaI CCWGG 1 cut(s) 165
MvnI CGCG 1 cut(s) 33
MwoI GCNNNNNNNGC 3 cut(s) 11, 30, 470
NcoI CCATGG 1 cut(s) 246
NdeII GATC 2 cut(s) 139, 225
NlaIII CATG 5 cut(s) 85, 196, 225, 250, 399
NspI RCATGY 1 cut(s) 399
PdmI GAANNNNTTC 1 cut(s) 159
PfoI TCCNGGA 1 cut(s) 163
PkrI GCNGC 3 cut(s) 134, 463, 475
PleI GAGTC 2 cut(s) 245, 355
PpsI GAGTC 2 cut(s) 245, 355
Psp6I CCWGG 1 cut(s) 163
PspGI CCWGG 1 cut(s) 163
RsaI GTAC 1 cut(s) 275
RsaNI GTAC 1 cut(s) 274
RseI CAYNNNNRTG 1 cut(s) 267
SaqAI TTAA 1 cut(s) 447
SatI GCNGC 3 cut(s) 133, 462, 474
Sau3AI GATC 2 cut(s) 139, 225
SchI GAGTC 2 cut(s) 245, 356
ScrFI CCNGG 1 cut(s) 165
SetI ASST 8 cut(s) 61, 148, 221, 297, 330, 378, 394, 463
SfaNI GCATC 3 cut(s) 14, 105, 485
SfcI CTRYAG 2 cut(s) 63, 411
SmiMI CAYNNNNRTG 1 cut(s) 267
SmlI CTYRAG 1 cut(s) 201
SmoI CTYRAG 1 cut(s) 201
Sse9I AATT 1 cut(s) 316
SsiI CCGC 1 cut(s) 33
SspMI CTAG 4 cut(s) 104, 143, 356, 422
StyD4I CCNGG 1 cut(s) 163
StyI CCWWGG 1 cut(s) 246
TaaI ACNGT 2 cut(s) 40, 412
TaiI ACGT 1 cut(s) 297
TasI AATT 1 cut(s) 316
Tru1I TTAA 1 cut(s) 447
Tru9I TTAA 1 cut(s) 447
TscAI CASTG 3 cut(s) 142, 327, 415
TseI GCWGC 3 cut(s) 132, 461, 473
TspDTI ATGAA 1 cut(s) 181
TspRI CASTG 3 cut(s) 142, 327, 415
XapI RAATTY 1 cut(s) 316
XceI RCATGY 1 cut(s) 399
XmnI GAANNNNTTC 1 cut(s) 159
XspI CTAG 4 cut(s) 104, 143, 356, 422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.