RLG00000027138

Polyketide cyclase / dehydrase and lipid transport

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0017716
Physical Location & Seq
Reverse (-)
8882533 .. 8883110
578 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027138

Sequence Viewer

Length: 489 bp
ATGGCATCAGAGGCCAGAAGTGAGAAATGGCGCGGATCAGTTGGCGGAATTGTTGAAGCTCCCATAGAAAAAGTCTGGTGCATGGTGTCACAAACCAAAAGACTAGCAGAGTGGATGCCAATGGTTGAGCGCTGCACTGATTTAGCCGGAGAGGAAGGTGTTCCTGGTTATGTTCGACTAGTCTCGGGATTCATGTTCCCCCAAGAAGATGGAGGAAGGTCATGGATCAAAGAGAAGCTGCTGTCCATAAATTCATCACAACATAGATATGTTTACAAATTGGAGGCAAGCAATGTAGGCTTAGATGGGTCTGTCAATTCAGTCAAGCTTGTAGATTATGGGAATGATTCGACACTAGTTGATTGGTCATATGAGCTAAACCCCTTACAAGGTATATGTGAGGATAGCATTATAGACTATCTGGGATTTCTATACAAATCTTGTATTAACAGGATTGAGGGTGCTATAGAAGCATCCAGCAAGGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Polyketide_cyc2

Protein Analysis

163

Amino Acids

18.07

Weight (kDa)

4.89

Isoelectric Point (pI)

46.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Polyketide_cyc2 PF10604 18 - 131 2.1e-11 Polyketide cyclase / dehydrase and lipid transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017716)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g23970
malus_domestica MD01G1146400.v1.1
pyrus_communis pycom01g16580
rosa_chinensis RchiOBHm_Chr1g0369311
rosa_laevigata RLG00000027138
rosa_roxburghii Rroxscaffold_4G00287740
rosa_rugosa Rorug01G0349500
rosa_samantha Rh1AG356700 Rh1BG320700 Rh1CG334700 Rh1DG351400
rosa_wichuraiana Rw1G031490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 33
AciI CCGC 2 cut(s) 33, 45
AclWI GGATC 2 cut(s) 43, 233
AcsI RAATTY 1 cut(s) 250
AfeI AGCGCT 1 cut(s) 131
AfiI CCNNNNNNNGG 1 cut(s) 389
AgsI TTSAA 1 cut(s) 56
AhlI ACTAGT 2 cut(s) 178, 355
AjnI CCWGG 1 cut(s) 163
AluBI AGCT 4 cut(s) 59, 238, 328, 376
AluI AGCT 4 cut(s) 59, 238, 328, 376
Alw26I GTCTC 1 cut(s) 187
AlwI GGATC 2 cut(s) 43, 233
Ama87I CYCGRG 1 cut(s) 184
Aor51HI AGCGCT 1 cut(s) 131
AoxI GGCC 1 cut(s) 12
ApeKI GCWGC 2 cut(s) 132, 238
ApoI RAATTY 1 cut(s) 250
Asp700I GAANNNNTTC 1 cut(s) 159
AspLEI GCGC 2 cut(s) 33, 132
AvaI CYCGRG 1 cut(s) 184
BbvI GCAGC 2 cut(s) 119, 225
BccI CCATC 2 cut(s) 203, 299
BciT130I CCWGG 1 cut(s) 165
BcoDI GTCTC 1 cut(s) 187
BcuI ACTAGT 2 cut(s) 178, 355
BfaI CTAG 3 cut(s) 104, 179, 356
BfmI CTRYAG 1 cut(s) 465
BfoI RGCGCY 1 cut(s) 133
BisI GCNGC 2 cut(s) 133, 239
BlsI GCNGC 2 cut(s) 134, 240
Bme1390I CCNGG 1 cut(s) 165
BmeT110I CYCGRG 1 cut(s) 184
BmrFI CCNGG 1 cut(s) 165
BmsI GCATC 3 cut(s) 14, 105, 482
Bsc4I CCNNNNNNNGG 1 cut(s) 389
Bse3DI GCAATG 1 cut(s) 298
BseBI CCWGG 1 cut(s) 165
BseGI GGATG 2 cut(s) 120, 473
BseLI CCNNNNNNNGG 1 cut(s) 389
BseMI GCAATG 1 cut(s) 298
BseXI GCAGC 2 cut(s) 119, 225
BsgI GTGCAG 1 cut(s) 118
Bsh1236I CGCG 1 cut(s) 33
BshFI GGCC 1 cut(s) 14
BsiHKCI CYCGRG 1 cut(s) 184
BsiSI CCGG 1 cut(s) 147
BslI CCNNNNNNNGG 1 cut(s) 389
BsmAI GTCTC 1 cut(s) 187
BsnI GGCC 1 cut(s) 14
BsoBI CYCGRG 1 cut(s) 184
Bsp143I GATC 2 cut(s) 35, 225
BspACI CCGC 2 cut(s) 33, 45
BspANI GGCC 1 cut(s) 14
BspFNI CGCG 1 cut(s) 33
BspPI GGATC 2 cut(s) 43, 233
BsrDI GCAATG 1 cut(s) 298
BssMI GATC 2 cut(s) 35, 225
Bst2UI CCWGG 1 cut(s) 165
BstC8I GCNNGC 1 cut(s) 289
BstDEI CTNAG 1 cut(s) 301
BstF5I GGATG 2 cut(s) 120, 473
BstFNI CGCG 1 cut(s) 33
BstH2I RGCGCY 1 cut(s) 133
BstHHI GCGC 2 cut(s) 33, 132
BstKTI GATC 2 cut(s) 38, 228
BstMAI GTCTC 1 cut(s) 187
BstMBI GATC 2 cut(s) 35, 225
BstMWI GCNNNNNNNGC 3 cut(s) 11, 297, 470
BstNI CCWGG 1 cut(s) 165
BstSCI CCNGG 1 cut(s) 163
BstSFI CTRYAG 1 cut(s) 465
BstUI CGCG 1 cut(s) 33
BstV1I GCAGC 2 cut(s) 119, 225
BstXI CCANNNNNNTGG 1 cut(s) 209
BsuRI GGCC 1 cut(s) 14
BtsCI GGATG 2 cut(s) 120, 473
BtsIMutI CAGTG 1 cut(s) 135
Cac8I GCNNGC 1 cut(s) 289
CfoI GCGC 2 cut(s) 33, 132
CviAII CATG 3 cut(s) 82, 193, 222
CviJI RGCY 7 cut(s) 14, 59, 146, 238, 300, 328, 376
CviKI_1 RGCY 7 cut(s) 14, 59, 146, 238, 300, 328, 376
DdeI CTNAG 1 cut(s) 301
DpnI GATC 2 cut(s) 37, 227
DpnII GATC 2 cut(s) 35, 225
EciI GGCGGA 1 cut(s) 60
Eco47III AGCGCT 1 cut(s) 131
Eco88I CYCGRG 1 cut(s) 184
EcoRII CCWGG 1 cut(s) 163
FaeI CATG 3 cut(s) 85, 196, 225
FatI CATG 3 cut(s) 81, 192, 221
FauNDI CATATG 1 cut(s) 370
Fnu4HI GCNGC 2 cut(s) 133, 239
FokI GGATG 2 cut(s) 127, 460
Fsp4HI GCNGC 2 cut(s) 133, 239
FspBI CTAG 3 cut(s) 104, 179, 356
GlaI GCGC 2 cut(s) 32, 131
GluI GCNGC 2 cut(s) 133, 239
HaeII RGCGCY 1 cut(s) 133
HaeIII GGCC 1 cut(s) 14
HapII CCGG 1 cut(s) 147
HhaI GCGC 2 cut(s) 33, 132
Hin1II CATG 3 cut(s) 85, 196, 225
Hin6I GCGC 2 cut(s) 31, 130
HinP1I GCGC 2 cut(s) 31, 130
HindIII AAGCTT 1 cut(s) 326
HinfI GANTC 2 cut(s) 189, 347
HpaII CCGG 1 cut(s) 147
Hpy166II GTNNAC 1 cut(s) 274
Hpy188I TCNGA 2 cut(s) 10, 488
Hpy188III TCNNGA 1 cut(s) 186
Hpy8I GTNNAC 1 cut(s) 274
HpyAV CCTTC 2 cut(s) 149, 210
HpyCH4V TGCA 2 cut(s) 81, 135
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 297, 470
HpyF3I CTNAG 1 cut(s) 301
Hsp92II CATG 3 cut(s) 85, 196, 225
HspAI GCGC 2 cut(s) 31, 130
Kzo9I GATC 2 cut(s) 35, 225
LmnI GCTCC 1 cut(s) 64
LpnPI CCDG 7 cut(s) 28, 61, 150, 160, 177, 407, 436
Lsp1109I GCAGC 2 cut(s) 119, 225
LweI GCATC 3 cut(s) 14, 105, 482
MaeI CTAG 3 cut(s) 104, 179, 356
MaeIII GTNAC 1 cut(s) 87
MalI GATC 2 cut(s) 37, 227
MboI GATC 2 cut(s) 35, 225
MboII GAAGA 1 cut(s) 218
MluCI AATT 4 cut(s) 48, 250, 278, 316
MnlI CCTC 6 cut(s) 4, 145, 206, 277, 394, 451
MroXI GAANNNNTTC 1 cut(s) 159
MseI TTAA 1 cut(s) 447
MslI CAYNNNNRTG 1 cut(s) 267
MspI CCGG 1 cut(s) 147
MspR9I CCNGG 1 cut(s) 165
MvaI CCWGG 1 cut(s) 165
MvnI CGCG 1 cut(s) 33
MwoI GCNNNNNNNGC 3 cut(s) 11, 297, 470
NdeI CATATG 1 cut(s) 370
NdeII GATC 2 cut(s) 35, 225
NlaIII CATG 3 cut(s) 85, 196, 225
NmuCI GTSAC 1 cut(s) 87
PdmI GAANNNNTTC 1 cut(s) 159
PfeI GAWTC 2 cut(s) 189, 347
PkrI GCNGC 2 cut(s) 134, 240
Psp6I CCWGG 1 cut(s) 163
PspGI CCWGG 1 cut(s) 163
RseI CAYNNNNRTG 1 cut(s) 267
SaqAI TTAA 1 cut(s) 447
SatI GCNGC 2 cut(s) 133, 239
Sau3AI GATC 2 cut(s) 35, 225
ScrFI CCNGG 1 cut(s) 165
SetI ASST 8 cut(s) 61, 160, 221, 240, 330, 378, 394, 486
SfaNI GCATC 3 cut(s) 14, 105, 482
SfcI CTRYAG 1 cut(s) 465
SmiMI CAYNNNNRTG 1 cut(s) 267
SpeI ACTAGT 2 cut(s) 178, 355
Sse9I AATT 4 cut(s) 48, 250, 278, 316
SsiI CCGC 2 cut(s) 33, 45
SspMI CTAG 3 cut(s) 104, 179, 356
StyD4I CCNGG 1 cut(s) 163
TaqI TCGA 2 cut(s) 175, 350
TasI AATT 4 cut(s) 48, 250, 278, 316
TfiI GAWTC 2 cut(s) 189, 347
Tru1I TTAA 1 cut(s) 447
Tru9I TTAA 1 cut(s) 447
TscAI CASTG 1 cut(s) 142
TseFI GTSAC 1 cut(s) 87
TseI GCWGC 2 cut(s) 132, 238
Tsp45I GTSAC 1 cut(s) 87
TspDTI ATGAA 2 cut(s) 181, 243
TspRI CASTG 1 cut(s) 142
XapI RAATTY 1 cut(s) 250
XmnI GAANNNNTTC 1 cut(s) 159
XspI CTAG 3 cut(s) 104, 179, 356
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.