pycom01g18770

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0015695
Physical Location & Seq
Forward (+)
17612644 .. 17613648
1005 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g18770.1

Sequence Viewer

Length: 1005 bp
ATGGAAGAACAAGCTCAGCACTACACAAGCTGCTGCTCATTGACGATGAGTAATATTAGCAGTCCACATGTGATGCCGTGCTTAACAGAACAATGGGGAAATTTACCCTTCCAAGTCGACGATTCTGAGGACATGATCGTCTACAACTCCCTTCGTGATGCTTTTAGCCTCGGATGGTCTCCCGACCACACTTTTCCCTTCCAAGTCGACGATTGGGAGGACATGATCAACTACAACTCAGACGCTTTTAGCATCGGCTGGTCTCCCGACCACACCTTGGAATGCATCAAACCCGAACAAGCGTATGATCTTGATCATCTTTTTGATCAATATCATGATCAGCTGCCACCTGATCTTTTCACTCAATCTCAACCCCACGATTTTGATGTTTCCATTAATGGAAACAAACCCTCACAAACCCCAGTACTCCCACCTGATACTGATATTCCTCCGTTGGATCATGACGATCGTGATAAAAATCAAGTACAAGTAGGAGATGCAGTAGCTGAACAAGAGGAAGTGAATGTGAATGTCCCGAAATTACAAAAGTCGGGGGTGGCGAGAGGGAAGCATTATCGAGGGGTGAGGCAGAGGCCGTGGGGGAAATTCGCGGCAGAGATTCGTGACCCGGCTAAGAATGGGGCCAGAGTGTGGCTTGGTACCTACGAGACTTCCGAGGAAGCTGCTTTGGCTTACGACCGCGCTGCCTTTCGCATTCGTGGCTCCAAGGCTCTGCTCAATTTTCCTCACCGGATTGGGTTGAATGAGCCTCCGCCTGTTCGAGTCACCGGCAAGCGAAAAGAGCCAGAGCAAGAGGCCGCCGCCAGCAGTAGCACTTCTTCTTCTTCTTCAGCGACGACAACGGCGGCCACCAAGAGGTCCAAATTAGACAAGTCGGTCTCCTCGTCAGCAGATCATGATGATCAAGCAAATGTGTGTCACACAAGGCAAAAGAATAATACCTCATATATGCTGCCACTTGGCGAGCAACTATTGGTTAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 ko04626 map04075 map04626
Pfam Domains
Protein Families
AP2

Protein Analysis

335

Amino Acids

37.36

Weight (kDa)

5.01

Isoelectric Point (pI)

54.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 191 - 241 1.8e-15 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015695)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g26920
malus_domestica MD01G1177000.v1.1
prunus_persica Prupe.2G272300_v2.0.a1 Prupe.3G108800_v2.0.a1
pyrus_communis pycom01g18770
rosa_chinensis RchiOBHm_Chr1g0373621
rosa_laevigata RLG00000026812
rosa_multiflora Rmu_co8453887.1_g000001 Rmu_sc0013090.1_g000004
rosa_roxburghii Rroxscaffold_4G00284110
rosa_rugosa Rorug01G0378500.1
rosa_samantha Rh1AG387900 Rh1BG352300 Rh1CG365100 Rh1DG382800
rosa_wichuraiana Rw1G034730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 137, 896
Acc65I GGTACC 1 cut(s) 659
AccB1I GGYRCC 1 cut(s) 659
AccB7I CCANNNNNTGG 2 cut(s) 277, 651
AccI GTMKAC 3 cut(s) 117, 141, 207
AccII CGCG 2 cut(s) 611, 702
AciI CCGC 6 cut(s) 611, 700, 773, 819, 822, 866
AclWI GGATC 1 cut(s) 465
AcoI YGGCCR 1 cut(s) 867
AcsI RAATTY 2 cut(s) 100, 605
AcuI CTGAAG 1 cut(s) 834
AfaI GTAC 3 cut(s) 426, 486, 661
AfiI CCNNNNNNNGG 3 cut(s) 277, 651, 876
AflIII ACRYGT 1 cut(s) 67
AgsI TTSAA 1 cut(s) 763
AluBI AGCT 6 cut(s) 14, 30, 343, 506, 683, 1002
AluI AGCT 6 cut(s) 14, 30, 343, 506, 683, 1002
Alw26I GTCTC 4 cut(s) 183, 267, 662, 904
AlwI GGATC 1 cut(s) 465
AlwNI CAGNNNCTG 1 cut(s) 506
AoxI GGCC 4 cut(s) 593, 642, 816, 867
ApeKI GCWGC 6 cut(s) 30, 33, 343, 683, 704, 973
ApoI RAATTY 2 cut(s) 100, 605
AseI ATTAAT 1 cut(s) 396
Asp718I GGTACC 1 cut(s) 659
AspLEI GCGC 1 cut(s) 704
AspS9I GGNCC 2 cut(s) 642, 879
AsuC2I CCSGG 1 cut(s) 629
AsuHPI GGTGA 3 cut(s) 595, 740, 778
AvaII GGWCC 1 cut(s) 879
BanI GGYRCC 1 cut(s) 659
BarI GAAGNNNNNNTAC 2 cut(s) 823, 855
BbvI GCAGC 6 cut(s) 17, 20, 330, 670, 691, 960
BccI CCATC 1 cut(s) 168
BceAI ACGGC 3 cut(s) 61, 580, 879
BcgI CGANNNNNNTGC 4 cut(s) 665, 686, 699, 720
BclI TGATCA 5 cut(s) 225, 313, 325, 337, 922
BcnI CCSGG 1 cut(s) 629
BcoDI GTCTC 4 cut(s) 183, 267, 662, 904
BlpI GCTNAGC 1 cut(s) 15
BmcAI AGTACT 1 cut(s) 426
Bme1390I CCNGG 1 cut(s) 629
Bme18I GGWCC 1 cut(s) 879
BmgT120I GGNCC 2 cut(s) 642, 879
BmiI GGNNCC 3 cut(s) 643, 661, 724
BmrFI CCNGG 1 cut(s) 629
BmrI ACTGGG 1 cut(s) 416
BmsI GCATC 5 cut(s) 63, 148, 261, 294, 487
BmuI ACTGGG 1 cut(s) 416
Bpu1102I GCTNAGC 1 cut(s) 15
BpuMI CCSGG 1 cut(s) 629
BsaBI GATNNNNATC 3 cut(s) 312, 330, 477
BsaI GGTCTC 3 cut(s) 183, 267, 904
BsaJI CCNNGG 5 cut(s) 169, 276, 596, 675, 726
BsaWI WCCGGW 1 cut(s) 750
BsaXI ACNNNNNCTCC 2 cut(s) 486, 516
Bsc4I CCNNNNNNNGG 3 cut(s) 277, 651, 876
Bse118I RCCGGY 1 cut(s) 788
Bse1I ACTGG 1 cut(s) 422
Bse8I GATNNNNATC 3 cut(s) 312, 330, 477
BseDI CCNNGG 5 cut(s) 169, 276, 596, 675, 726
BseGI GGATG 1 cut(s) 179
BseJI GATNNNNATC 3 cut(s) 312, 330, 477
BseLI CCNNNNNNNGG 3 cut(s) 277, 651, 876
BseMII CTCAG 3 cut(s) 29, 117, 252
BseNI ACTGG 1 cut(s) 422
BseRI GAGGAG 1 cut(s) 892
BseXI GCAGC 6 cut(s) 17, 20, 330, 670, 691, 960
Bsh1236I CGCG 2 cut(s) 611, 702
Bsh1285I CGRYCG 2 cut(s) 469, 700
BshFI GGCC 4 cut(s) 595, 644, 818, 869
BshNI GGYRCC 1 cut(s) 659
BsiEI CGRYCG 2 cut(s) 469, 700
BsiSI CCGG 3 cut(s) 629, 751, 789
BslFI GGGAC 1 cut(s) 518
BslI CCNNNNNNNGG 3 cut(s) 277, 651, 876
BsmAI GTCTC 4 cut(s) 183, 267, 662, 904
BsmFI GGGAC 1 cut(s) 518
BsmI GAATGC 2 cut(s) 287, 714
BsnI GGCC 4 cut(s) 595, 644, 818, 869
Bso31I GGTCTC 3 cut(s) 183, 267, 904
Bsp1720I GCTNAGC 1 cut(s) 15
BspACI CCGC 6 cut(s) 611, 700, 773, 819, 822, 866
BspANI GGCC 4 cut(s) 595, 644, 818, 869
BspCNI CTCAG 3 cut(s) 28, 118, 251
BspFNI CGCG 2 cut(s) 611, 702
BspHI TCATGA 3 cut(s) 334, 460, 916
BspLI GGNNCC 3 cut(s) 643, 661, 724
BspPI GGATC 1 cut(s) 465
BspT107I GGYRCC 1 cut(s) 659
BspTNI GGTCTC 3 cut(s) 183, 267, 904
BsrFI RCCGGY 1 cut(s) 788
BsrI ACTGG 1 cut(s) 422
BssAI RCCGGY 1 cut(s) 788
BssECI CCNNGG 5 cut(s) 169, 276, 596, 675, 726
BssT1I CCWWGG 2 cut(s) 276, 726
BstC8I GCNNGC 3 cut(s) 794, 826, 986
BstDEI CTNAG 4 cut(s) 15, 126, 238, 633
BstDSI CCRYGG 1 cut(s) 596
BstF5I GGATG 1 cut(s) 179
BstFNI CGCG 2 cut(s) 611, 702
BstHHI GCGC 1 cut(s) 704
BstMAI GTCTC 4 cut(s) 183, 267, 662, 904
BstMCI CGRYCG 2 cut(s) 469, 700
BstMWI GCNNNNNNNGC 3 cut(s) 689, 720, 802
BstNSI RCATGY 1 cut(s) 71
BstSCI CCNGG 1 cut(s) 627
BstUI CGCG 2 cut(s) 611, 702
BstV1I GCAGC 6 cut(s) 17, 20, 330, 670, 691, 960
BsuRI GGCC 4 cut(s) 595, 644, 818, 869
BtgI CCRYGG 1 cut(s) 596
BtsCI GGATG 1 cut(s) 179
Cac8I GCNNGC 3 cut(s) 794, 826, 986
CaiI CAGNNNCTG 1 cut(s) 506
CciI TCATGA 3 cut(s) 334, 460, 916
CfoI GCGC 1 cut(s) 704
Cfr10I RCCGGY 1 cut(s) 788
Cfr13I GGNCC 2 cut(s) 642, 879
CseI GACGC 1 cut(s) 251
Csp6I GTAC 3 cut(s) 425, 485, 660
CviAII CATG 6 cut(s) 68, 133, 223, 335, 461, 917
CviQI GTAC 3 cut(s) 425, 485, 660
DdeI CTNAG 4 cut(s) 15, 126, 238, 633
DrdI GACNNNNNNGTC 2 cut(s) 137, 896
DseDI GACNNNNNNGTC 2 cut(s) 137, 896
EaeI YGGCCR 1 cut(s) 867
EciI GGCGGA 1 cut(s) 762
Eco130I CCWWGG 2 cut(s) 276, 726
Eco31I GGTCTC 3 cut(s) 183, 267, 904
Eco47I GGWCC 1 cut(s) 879
Eco57I CTGAAG 1 cut(s) 834
EcoT14I CCWWGG 2 cut(s) 276, 726
EcoT22I ATGCAT 1 cut(s) 287
ErhI CCWWGG 2 cut(s) 276, 726
FaeI CATG 6 cut(s) 71, 136, 226, 338, 464, 920
FaqI GGGAC 1 cut(s) 518
FatI CATG 6 cut(s) 67, 132, 222, 334, 460, 916
FbaI TGATCA 5 cut(s) 225, 313, 325, 337, 922
FblI GTMKAC 3 cut(s) 117, 141, 207
FokI GGATG 1 cut(s) 186
GlaI GCGC 1 cut(s) 703
HaeIII GGCC 4 cut(s) 595, 644, 818, 869
HapII CCGG 3 cut(s) 629, 751, 789
HgaI GACGC 1 cut(s) 251
HhaI GCGC 1 cut(s) 704
Hin1II CATG 6 cut(s) 71, 136, 226, 338, 464, 920
Hin6I GCGC 1 cut(s) 702
HinP1I GCGC 1 cut(s) 702
HincII GTYRAC 2 cut(s) 118, 208
HindII GTYRAC 2 cut(s) 118, 208
HinfI GANTC 3 cut(s) 122, 619, 783
HpaII CCGG 3 cut(s) 629, 751, 789
HphI GGTGA 3 cut(s) 595, 740, 778
Hpy166II GTNNAC 4 cut(s) 65, 118, 142, 208
Hpy188I TCNGA 4 cut(s) 127, 173, 241, 676
Hpy8I GTNNAC 4 cut(s) 65, 118, 142, 208
Hpy99I CGWCG 3 cut(s) 122, 212, 859
HpyAV CCTTC 3 cut(s) 118, 161, 208
HpyCH4V TGCA 2 cut(s) 285, 500
HpyF10VI GCNNNNNNNGC 3 cut(s) 689, 720, 802
HpyF3I CTNAG 4 cut(s) 15, 126, 238, 633
Hsp92II CATG 6 cut(s) 71, 136, 226, 338, 464, 920
HspAI GCGC 1 cut(s) 702
KpnI GGTACC 1 cut(s) 663
Ksp22I TGATCA 5 cut(s) 225, 313, 325, 337, 922
LmnI GCTCC 1 cut(s) 728
Lsp1109I GCAGC 6 cut(s) 17, 20, 330, 670, 691, 960
LweI GCATC 5 cut(s) 63, 148, 261, 294, 487
MaeIII GTNAC 3 cut(s) 623, 784, 938
MboII GAAGA 5 cut(s) 17, 831, 834, 837, 840
MluCI AATT 5 cut(s) 100, 539, 605, 739, 884
MlyI GAGTC 1 cut(s) 792
MmeI TCCRAC 1 cut(s) 435
Mph1103I ATGCAT 1 cut(s) 287
MseI TTAA 2 cut(s) 83, 396
MspA1I CMGCKG 1 cut(s) 343
MspI CCGG 3 cut(s) 629, 751, 789
MspR9I CCNGG 1 cut(s) 629
Mva1269I GAATGC 2 cut(s) 287, 714
MvnI CGCG 2 cut(s) 611, 702
MwoI GCNNNNNNNGC 3 cut(s) 689, 720, 802
NciI CCSGG 1 cut(s) 629
NlaIII CATG 6 cut(s) 71, 136, 226, 338, 464, 920
NlaIV GGNNCC 3 cut(s) 643, 661, 724
NmuCI GTSAC 3 cut(s) 623, 784, 938
NsiI ATGCAT 1 cut(s) 287
NspI RCATGY 1 cut(s) 71
PagI TCATGA 3 cut(s) 334, 460, 916
PciI ACATGT 1 cut(s) 67
PcsI WCGNNNNNNNCGW 3 cut(s) 557, 672, 902
PctI GAATGC 2 cut(s) 287, 714
PfeI GAWTC 2 cut(s) 122, 619
PflMI CCANNNNNTGG 2 cut(s) 277, 651
Ple19I CGATCG 1 cut(s) 469
PleI GAGTC 1 cut(s) 791
PpsI GAGTC 1 cut(s) 791
PscI ACATGT 1 cut(s) 67
PshBI ATTAAT 1 cut(s) 396
PspN4I GGNNCC 3 cut(s) 643, 661, 724
PspPI GGNCC 2 cut(s) 642, 879
PstNI CAGNNNCTG 1 cut(s) 506
PvuI CGATCG 1 cut(s) 469
PvuII CAGCTG 1 cut(s) 343
RsaI GTAC 3 cut(s) 426, 486, 661
RsaNI GTAC 3 cut(s) 425, 485, 660
SalI GTCGAC 2 cut(s) 116, 206
SaqAI TTAA 2 cut(s) 83, 396
Sau96I GGNCC 2 cut(s) 642, 879
ScaI AGTACT 1 cut(s) 426
SchI GAGTC 1 cut(s) 792
ScrFI CCNGG 1 cut(s) 629
SfaNI GCATC 5 cut(s) 63, 148, 261, 294, 487
SinI GGWCC 1 cut(s) 879
Sse9I AATT 5 cut(s) 100, 539, 605, 739, 884
SsiI CCGC 6 cut(s) 611, 700, 773, 819, 822, 866
SspI AATATT 1 cut(s) 55
StyD4I CCNGG 1 cut(s) 627
StyI CCWWGG 2 cut(s) 276, 726
TaqI TCGA 4 cut(s) 117, 207, 577, 781
TaqII GACCGA 1 cut(s) 886
TasI AATT 5 cut(s) 100, 539, 605, 739, 884
TatI WGTACW 2 cut(s) 424, 484
TauI GCSGC 4 cut(s) 614, 821, 824, 869
TfiI GAWTC 2 cut(s) 122, 619
Tru1I TTAA 2 cut(s) 83, 396
Tru9I TTAA 2 cut(s) 83, 396
TseFI GTSAC 3 cut(s) 623, 784, 938
TseI GCWGC 6 cut(s) 30, 33, 343, 683, 704, 973
Tsp45I GTSAC 3 cut(s) 623, 784, 938
TspGWI ACGGA 1 cut(s) 441
Van91I CCANNNNNTGG 2 cut(s) 277, 651
VpaK11BI GGWCC 1 cut(s) 879
VspI ATTAAT 1 cut(s) 396
XapI RAATTY 2 cut(s) 100, 605
XceI RCATGY 1 cut(s) 71
XmiI GTMKAC 3 cut(s) 117, 141, 207
ZrmI AGTACT 1 cut(s) 426
Zsp2I ATGCAT 1 cut(s) 287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.