Rw1G034730

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0015695
Physical Location & Seq
Reverse (-)
62645505 .. 62646364
860 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G034730.1

Sequence Viewer

Length: 780 bp
ATGACAGAGCTAAATGGTCTTCACGATCATTTTCTTCCTCATCCAAACTTAATCATCGACCCTCTAACAGAACAGTGGGGAGACTTGCCCTTCATGTTCGACGATTTCGATGACATGCTCGTCTACGACTCCCTCTGGACCGCCTTCAGCTCCGATTTGTCCACCCCCTCCATGGAGGACTTAACCACCGCCGTCAAGCCCGAGCCGGCCTATAACTACTTCGATGAACTACATCAAATTTTGCAGCCTCAAGAGTCATCGATTCTACATGATCAAGTTTCCATCAACATCAGCGCGCCAAATGAACTATCATCACCGGAAACTGAATCAACAGTCTCTCAGACGAGCTATGATCAGCTACAAGAGGAAGTCCCTACCCCAAAAGTACAGAAGGTACTTGGCGGCGGTACGAGTAGCGTTTCAAGAGGGAGGCATTACAGAGGGGTGAGACAGCGGCCGTGGGGAAAGTTCGCCGCCGAGATTCGAGATCCAGCGAAGAACGGGGCTCGAGTGTGGCTTGGCACCTATGAGACCTCCGAGGAAGCGGCTGTGGCTTATGACCGAGCCGCTTACCGGATGCGTGACCCTGATCAACAAGCCGGAAACAGTCCCACATCGACGACTACAAGAACGCCGAACTTGAATAAGAGGACCAAAGCCAGCTTGGCGTCGCCGGCGCCTGATATGGGAGCTGATGCTAAGTTTGAAAGAGATGATCGTACCAGAGTAAATGTCAATCGTACAAGACATAATAGTAATGTGGGTTTACTAGTCAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 ko04626 map04075 map04626
Pfam Domains
Protein Families
AP2

Protein Analysis

259

Amino Acids

29.01

Weight (kDa)

5.0

Isoelectric Point (pI)

51.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 145 - 194 6.3e-14 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015695)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g26920
malus_domestica MD01G1177000.v1.1
prunus_persica Prupe.2G272300_v2.0.a1 Prupe.3G108800_v2.0.a1
pyrus_communis pycom01g18770
rosa_chinensis RchiOBHm_Chr1g0373621
rosa_laevigata RLG00000026812
rosa_multiflora Rmu_co8453887.1_g000001 Rmu_sc0013090.1_g000004
rosa_roxburghii Rroxscaffold_4G00284110
rosa_rugosa Rorug01G0378500.1
rosa_samantha Rh1AG387900 Rh1BG352300 Rh1CG365100 Rh1DG382800
rosa_wichuraiana Rw1G034730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 119
AccB1I GGYRCC 2 cut(s) 521, 676
AccI GTMKAC 1 cut(s) 123
AccII CGCG 1 cut(s) 296
AciI CCGC 8 cut(s) 141, 189, 402, 405, 454, 474, 545, 567
AclWI GGATC 1 cut(s) 482
AcoI YGGCCR 1 cut(s) 455
AcsI RAATTY 1 cut(s) 237
AcuI CTGAAG 1 cut(s) 130
AcyI GRCGYC 2 cut(s) 668, 677
AfaI GTAC 5 cut(s) 387, 396, 409, 721, 742
AfiI CCNNNNNNNGG 3 cut(s) 172, 573, 686
AgsI TTSAA 3 cut(s) 423, 643, 707
AhlI ACTAGT 1 cut(s) 769
AluBI AGCT 6 cut(s) 10, 150, 348, 358, 663, 692
AluI AGCT 6 cut(s) 10, 150, 348, 358, 663, 692
Alw26I GTCTC 4 cut(s) 75, 340, 442, 524
AlwI GGATC 1 cut(s) 482
Ama87I CYCGRG 2 cut(s) 200, 507
AoxI GGCC 2 cut(s) 207, 455
ApeKI GCWGC 1 cut(s) 244
ApoI RAATTY 1 cut(s) 237
AspLEI GCGC 3 cut(s) 296, 298, 679
AspS9I GGNCC 2 cut(s) 138, 651
AsuHPI GGTGA 2 cut(s) 306, 457
AvaI CYCGRG 2 cut(s) 200, 507
AvaII GGWCC 2 cut(s) 138, 651
BanI GGYRCC 2 cut(s) 521, 676
BanII GRGCYC 1 cut(s) 508
BbsI GAAGAC 1 cut(s) 11
BbvI GCAGC 1 cut(s) 256
BccI CCATC 1 cut(s) 290
BceAI ACGGC 2 cut(s) 176, 442
BclI TGATCA 3 cut(s) 271, 352, 589
BcoDI GTCTC 4 cut(s) 75, 340, 442, 524
BcuI ACTAGT 1 cut(s) 769
BfaI CTAG 1 cut(s) 770
BfoI RGCGCY 1 cut(s) 680
BglI GCCNNNNNGGC 1 cut(s) 665
BisI GCNGC 6 cut(s) 245, 403, 455, 474, 546, 567
BlsI GCNGC 6 cut(s) 246, 404, 456, 475, 547, 568
Bme18I GGWCC 2 cut(s) 138, 651
BmeT110I CYCGRG 2 cut(s) 200, 507
BmgT120I GGNCC 2 cut(s) 138, 651
BmiI GGNNCC 2 cut(s) 523, 678
BmsI GCATC 2 cut(s) 567, 685
BpiI GAAGAC 1 cut(s) 11
BpuEI CTTGAG 1 cut(s) 234
Bsa29I ATCGAT 1 cut(s) 260
BsaHI GRCGYC 2 cut(s) 668, 677
BsaI GGTCTC 1 cut(s) 524
BsaJI CCNNGG 3 cut(s) 171, 458, 537
BsaWI WCCGGW 2 cut(s) 316, 573
Bsc4I CCNNNNNNNGG 3 cut(s) 172, 573, 686
Bse118I RCCGGY 2 cut(s) 205, 673
BseCI ATCGAT 1 cut(s) 260
BseDI CCNNGG 3 cut(s) 171, 458, 537
BseGI GGATG 2 cut(s) 40, 582
BseLI CCNNNNNNNGG 3 cut(s) 172, 573, 686
BseMII CTCAG 1 cut(s) 353
BsePI GCGCGC 1 cut(s) 294
BseX3I CGGCCG 1 cut(s) 455
BseXI GCAGC 1 cut(s) 256
Bsh1236I CGCG 1 cut(s) 296
Bsh1285I CGRYCG 1 cut(s) 458
BshFI GGCC 2 cut(s) 209, 457
BshNI GGYRCC 2 cut(s) 521, 676
BshVI ATCGAT 1 cut(s) 260
BsiEI CGRYCG 1 cut(s) 458
BsiHKCI CYCGRG 2 cut(s) 200, 507
BsiSI CCGG 5 cut(s) 206, 317, 574, 600, 674
BslFI GGGAC 2 cut(s) 356, 594
BslI CCNNNNNNNGG 3 cut(s) 172, 573, 686
BsmAI GTCTC 4 cut(s) 75, 340, 442, 524
BsmFI GGGAC 2 cut(s) 356, 594
BsnI GGCC 2 cut(s) 209, 457
Bso31I GGTCTC 1 cut(s) 524
BsoBI CYCGRG 2 cut(s) 200, 507
Bsp1286I GDGCHC 1 cut(s) 508
Bsp143I GATC 6 cut(s) 25, 271, 352, 487, 589, 715
Bsp19I CCATGG 1 cut(s) 171
BspACI CCGC 8 cut(s) 141, 189, 402, 405, 454, 474, 545, 567
BspANI GGCC 2 cut(s) 209, 457
BspCNI CTCAG 1 cut(s) 352
BspDI ATCGAT 1 cut(s) 260
BspFNI CGCG 1 cut(s) 296
BspLI GGNNCC 2 cut(s) 523, 678
BspPI GGATC 1 cut(s) 482
BspT107I GGYRCC 2 cut(s) 521, 676
BspTNI GGTCTC 1 cut(s) 524
BsrFI RCCGGY 2 cut(s) 205, 673
BssAI RCCGGY 2 cut(s) 205, 673
BssECI CCNNGG 3 cut(s) 171, 458, 537
BssHII GCGCGC 1 cut(s) 294
BssMI GATC 6 cut(s) 25, 271, 352, 487, 589, 715
BssNI GRCGYC 2 cut(s) 668, 677
BssT1I CCWWGG 1 cut(s) 171
Bst4CI ACNGT 3 cut(s) 75, 334, 608
BstACI GRCGYC 2 cut(s) 668, 677
BstC8I GCNNGC 4 cut(s) 207, 296, 661, 675
BstDEI CTNAG 2 cut(s) 339, 699
BstDSI CCRYGG 2 cut(s) 171, 458
BstF5I GGATG 2 cut(s) 40, 582
BstFNI CGCG 1 cut(s) 296
BstH2I RGCGCY 1 cut(s) 680
BstHHI GCGC 3 cut(s) 296, 298, 679
BstKTI GATC 6 cut(s) 28, 274, 355, 490, 592, 718
BstMAI GTCTC 4 cut(s) 75, 340, 442, 524
BstMBI GATC 6 cut(s) 25, 271, 352, 487, 589, 715
BstMCI CGRYCG 1 cut(s) 458
BstMWI GCNNNNNNNGC 3 cut(s) 551, 665, 674
BstNSI RCATGY 1 cut(s) 118
BstUI CGCG 1 cut(s) 296
BstV1I GCAGC 1 cut(s) 256
BstV2I GAAGAC 1 cut(s) 11
BstX2I RGATCY 1 cut(s) 487
BstYI RGATCY 1 cut(s) 487
BstZI CGGCCG 1 cut(s) 455
Bsu15I ATCGAT 1 cut(s) 260
BsuRI GGCC 2 cut(s) 209, 457
BsuTUI ATCGAT 1 cut(s) 260
BtgI CCRYGG 2 cut(s) 171, 458
BtsCI GGATG 2 cut(s) 40, 582
BtsIMutI CAGTG 1 cut(s) 80
Cac8I GCNNGC 4 cut(s) 207, 296, 661, 675
CfoI GCGC 3 cut(s) 296, 298, 679
Cfr10I RCCGGY 2 cut(s) 205, 673
Cfr13I GGNCC 2 cut(s) 138, 651
ClaI ATCGAT 1 cut(s) 260
CseI GACGC 1 cut(s) 657
Csp6I GTAC 5 cut(s) 386, 395, 408, 720, 741
CviAII CATG 4 cut(s) 94, 115, 172, 269
CviQI GTAC 5 cut(s) 386, 395, 408, 720, 741
DdeI CTNAG 2 cut(s) 339, 699
DinI GGCGCC 1 cut(s) 678
DpnI GATC 6 cut(s) 27, 273, 354, 489, 591, 717
DpnII GATC 6 cut(s) 25, 271, 352, 487, 589, 715
DrdI GACNNNNNNGTC 1 cut(s) 119
DseDI GACNNNNNNGTC 1 cut(s) 119
EaeI YGGCCR 1 cut(s) 455
EagI CGGCCG 1 cut(s) 455
EclXI CGGCCG 1 cut(s) 455
Eco130I CCWWGG 1 cut(s) 171
Eco24I GRGCYC 1 cut(s) 508
Eco31I GGTCTC 1 cut(s) 524
Eco47I GGWCC 2 cut(s) 138, 651
Eco52I CGGCCG 1 cut(s) 455
Eco57I CTGAAG 1 cut(s) 130
Eco88I CYCGRG 2 cut(s) 200, 507
EcoT14I CCWWGG 1 cut(s) 171
EcoT38I GRGCYC 1 cut(s) 508
EgeI GGCGCC 1 cut(s) 678
EheI GGCGCC 1 cut(s) 678
ErhI CCWWGG 1 cut(s) 171
FaeI CATG 4 cut(s) 97, 118, 175, 272
FaqI GGGAC 2 cut(s) 356, 594
FatI CATG 4 cut(s) 93, 114, 171, 268
FbaI TGATCA 3 cut(s) 271, 352, 589
FblI GTMKAC 1 cut(s) 123
Fnu4HI GCNGC 6 cut(s) 245, 403, 455, 474, 546, 567
FokI GGATG 2 cut(s) 27, 589
FriOI GRGCYC 1 cut(s) 508
Fsp4HI GCNGC 6 cut(s) 245, 403, 455, 474, 546, 567
FspBI CTAG 1 cut(s) 770
GlaI GCGC 3 cut(s) 295, 297, 678
GluI GCNGC 6 cut(s) 245, 403, 455, 474, 546, 567
HaeII RGCGCY 1 cut(s) 680
HaeIII GGCC 2 cut(s) 209, 457
HapII CCGG 5 cut(s) 206, 317, 574, 600, 674
HgaI GACGC 1 cut(s) 657
HhaI GCGC 3 cut(s) 296, 298, 679
Hin1I GRCGYC 2 cut(s) 668, 677
Hin1II CATG 4 cut(s) 97, 118, 175, 272
Hin6I GCGC 3 cut(s) 294, 296, 677
HinP1I GCGC 3 cut(s) 294, 296, 677
HincII GTYRAC 1 cut(s) 775
HindII GTYRAC 1 cut(s) 775
HinfI GANTC 5 cut(s) 128, 254, 262, 326, 481
HpaII CCGG 5 cut(s) 206, 317, 574, 600, 674
HphI GGTGA 2 cut(s) 306, 457
Hpy166II GTNNAC 4 cut(s) 124, 162, 767, 775
Hpy188I TCNGA 3 cut(s) 154, 342, 538
Hpy188III TCNNGA 5 cut(s) 23, 136, 251, 423, 485
Hpy8I GTNNAC 4 cut(s) 124, 162, 767, 775
Hpy99I CGWCG 3 cut(s) 104, 622, 673
HpyAV CCTTC 3 cut(s) 100, 154, 385
HpyCH4III ACNGT 3 cut(s) 75, 334, 608
HpyCH4V TGCA 1 cut(s) 244
HpyF10VI GCNNNNNNNGC 3 cut(s) 551, 665, 674
HpyF3I CTNAG 2 cut(s) 339, 699
Hsp92I GRCGYC 2 cut(s) 668, 677
Hsp92II CATG 4 cut(s) 97, 118, 175, 272
HspAI GCGC 3 cut(s) 294, 296, 677
KasI GGCGCC 1 cut(s) 676
KroI GCCGGC 2 cut(s) 205, 673
KroNI GCCGGC 2 cut(s) 207, 675
Ksp22I TGATCA 3 cut(s) 271, 352, 589
Kzo9I GATC 6 cut(s) 25, 271, 352, 487, 589, 715
LmnI GCTCC 2 cut(s) 155, 689
Lsp1109I GCAGC 1 cut(s) 256
LweI GCATC 2 cut(s) 567, 685
MaeI CTAG 1 cut(s) 770
MaeIII GTNAC 1 cut(s) 581
MalI GATC 6 cut(s) 27, 273, 354, 489, 591, 717
MboI GATC 6 cut(s) 25, 271, 352, 487, 589, 715
MboII GAAGA 3 cut(s) 11, 26, 508
MflI RGATCY 1 cut(s) 487
MhlI GDGCHC 1 cut(s) 508
MluCI AATT 1 cut(s) 237
Mly113I GGCGCC 1 cut(s) 677
MlyI GAGTC 2 cut(s) 122, 263
MreI CGCCGGCG 1 cut(s) 673
MroNI GCCGGC 2 cut(s) 205, 673
MseI TTAA 2 cut(s) 50, 182
MspA1I CMGCKG 1 cut(s) 454
MspI CCGG 5 cut(s) 206, 317, 574, 600, 674
MvnI CGCG 1 cut(s) 296
MwoI GCNNNNNNNGC 3 cut(s) 551, 665, 674
NaeI GCCGGC 2 cut(s) 207, 675
NarI GGCGCC 1 cut(s) 677
NcoI CCATGG 1 cut(s) 171
NdeII GATC 6 cut(s) 25, 271, 352, 487, 589, 715
NgoMIV GCCGGC 2 cut(s) 205, 673
NlaIII CATG 4 cut(s) 97, 118, 175, 272
NlaIV GGNNCC 2 cut(s) 523, 678
NmeAIII GCCGAG 1 cut(s) 502
NmuCI GTSAC 1 cut(s) 581
NspI RCATGY 1 cut(s) 118
PaeR7I CTCGAG 1 cut(s) 507
PauI GCGCGC 1 cut(s) 294
PcsI WCGNNNNNNNCGW 1 cut(s) 105
PdiI GCCGGC 2 cut(s) 207, 675
PfeI GAWTC 3 cut(s) 262, 326, 481
PkrI GCNGC 6 cut(s) 246, 404, 456, 475, 547, 568
PleI GAGTC 2 cut(s) 122, 262
PluTI GGCGCC 1 cut(s) 680
PpsI GAGTC 2 cut(s) 122, 262
PspN4I GGNNCC 2 cut(s) 523, 678
PspPI GGNCC 2 cut(s) 138, 651
PspXI VCTCGAGB 1 cut(s) 507
PsuI RGATCY 1 cut(s) 487
PteI GCGCGC 1 cut(s) 294
RsaI GTAC 5 cut(s) 387, 396, 409, 721, 742
RsaNI GTAC 5 cut(s) 386, 395, 408, 720, 741
SaqAI TTAA 2 cut(s) 50, 182
SatI GCNGC 6 cut(s) 245, 403, 455, 474, 546, 567
Sau3AI GATC 6 cut(s) 25, 271, 352, 487, 589, 715
Sau96I GGNCC 2 cut(s) 138, 651
SchI GAGTC 2 cut(s) 122, 263
SduI GDGCHC 1 cut(s) 508
SetI ASST 9 cut(s) 12, 152, 350, 360, 396, 527, 536, 665, 694
SfaNI GCATC 2 cut(s) 567, 685
SfoI GGCGCC 1 cut(s) 678
Sfr274I CTCGAG 1 cut(s) 507
SgrAI CRCCGGYG 1 cut(s) 673
SinI GGWCC 2 cut(s) 138, 651
SlaI CTCGAG 1 cut(s) 507
SmlI CTYRAG 2 cut(s) 249, 507
SmoI CTYRAG 2 cut(s) 249, 507
SpeI ACTAGT 1 cut(s) 769
Sse9I AATT 1 cut(s) 237
SsiI CCGC 8 cut(s) 141, 189, 402, 405, 454, 474, 545, 567
SspDI GGCGCC 1 cut(s) 676
SspMI CTAG 1 cut(s) 770
StyI CCWWGG 1 cut(s) 171
TaaI ACNGT 3 cut(s) 75, 334, 608
TaqI TCGA 8 cut(s) 57, 99, 108, 222, 260, 484, 508, 617
TaqII GACCGA 1 cut(s) 576
TasI AATT 1 cut(s) 237
TatI WGTACW 1 cut(s) 385
TauI GCSGC 5 cut(s) 405, 457, 476, 548, 569
TfiI GAWTC 3 cut(s) 262, 326, 481
Tru1I TTAA 2 cut(s) 50, 182
Tru9I TTAA 2 cut(s) 50, 182
TscAI CASTG 1 cut(s) 80
TseFI GTSAC 1 cut(s) 581
TseI GCWGC 1 cut(s) 244
Tsp45I GTSAC 1 cut(s) 581
TspDTI ATGAA 3 cut(s) 82, 240, 318
TspRI CASTG 1 cut(s) 80
VpaK11BI GGWCC 2 cut(s) 138, 651
XapI RAATTY 1 cut(s) 237
XceI RCATGY 1 cut(s) 118
XcmI CCANNNNNNNNNTGG 2 cut(s) 169, 661
XhoI CTCGAG 1 cut(s) 507
XmiI GTMKAC 1 cut(s) 123
XspI CTAG 1 cut(s) 770
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.