pycom01g22400

Belongs to the glycosyl hydrolase 18 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0019771
Physical Location & Seq
Reverse (-)
20007063 .. 20007956
894 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 894 bp
ATGGCAGCCAAAACACAAACCCTAGCCCTAACGCTGTCCCTCTTGATCCTCATTTCTTCATGCGAGTCCTCTCAAGCCGCGGGAATTGCAACGTATTGGGGCCAAAACGGCAACGAAGGAACCCTAGCAGATGCTTGCAACTCGGGCAACTACCAGTTTGTTAACATAGCTTTCCTCACGACTTTCGGAAACAATCAAGCCCCTGTCCTAAACCTCGCCGGCCACTGCGACCCCAGCAGTAGTACTTGCACGGGGCTGAGCGTCGACATCAGAGCTTGCCAATCCCAAAACATAAAAGTCCTCCTCTCGATTGGAGGGGCTGTCGGAAGTTACAATCTCACTTCAGCTGATGATGCAAGGCAAGTTGCTGATTACATCTGGAACAACTTCCTAGGTGGTCAGTCCGCTTCGCGCCCGCTTGGGGACGCTGTTTTGGACGGCGTTGATTTCGCTATTGTTATAGGTGGTGGGCAGTTCTATGATGAGCTCGCCAGGTCACTCAACGGACACAACGGACAGGCACAAACGGTCTATTTAGCCGCAGCTCCACAATGTCCAATCCCGGATGCTCACCTAGACGGCGCAATCCAAACCGGCTTATTTGACTACGTTTGGGTTCAGTTCTACAACAACCCCCCATGCCAGTATGCAGACGGTAATGCCGACGCTCTTTTGAGCAGGTGGAAACAATGGGCCTCGGTTCCAGCCAGCGAGGTGTTCTTGGGTTTACCGGCAGCTCCTGAGGCCGCTCCGAGCGGCGGATTTATTCCTGCTGATGCTCTCAAGTCACAAGTTCTTCCAACGATTAAGAATTCGCCCAAGTACGGAGGAGTTATGCTCTGGAGCAGGCAGTATGACAACGGTTATAGTGCATCCATTATGGACAACATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005615 GO:0044421
KEGG Pathways
Metabolic & Signaling
ko00520 ko01100 map00520 map01100
Pfam Domains
Protein Families
Glyco_hydro_18

Protein Analysis

298

Amino Acids

31.3

Weight (kDa)

4.42

Isoelectric Point (pI)

43.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_18 PF00704 29 - 246 2.5e-17 Glycosyl hydrolases family 18
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019771)

Species Orthologous Gene IDs
malus_domestica MD01G1212900.v1.1 MD01G1213100.v1.1 MD01G1213300.v1.1 MD07G1282900.v1.1
prunus_persica Prupe.2G305200_v2.0.a1
pyrus_communis pycom01g22400 pycom01g22420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 669
Acc36I ACCTGC 1 cut(s) 669
AccBSI CCGCTC 2 cut(s) 749, 756
AccI GTMKAC 1 cut(s) 264
AccII CGCG 2 cut(s) 80, 412
AciI CCGC 8 cut(s) 78, 80, 405, 416, 540, 747, 756, 759
AclWI GGATC 1 cut(s) 40
AcoI YGGCCR 1 cut(s) 220
AcsI RAATTY 1 cut(s) 811
AcuI CTGAAG 1 cut(s) 327
AfaI GTAC 2 cut(s) 244, 824
AfiI CCNNNNNNNGG 3 cut(s) 421, 758, 824
AjnI CCWGG 1 cut(s) 491
AluBI AGCT 6 cut(s) 170, 275, 347, 487, 545, 737
AluI AGCT 6 cut(s) 170, 275, 347, 487, 545, 737
Alw21I GWGCWC 1 cut(s) 489
AlwI GGATC 1 cut(s) 40
AlwNI CAGNNNCTG 1 cut(s) 740
Ama87I CYCGRG 1 cut(s) 142
AoxI GGCC 4 cut(s) 100, 220, 693, 744
ApeKI GCWGC 3 cut(s) 5, 542, 734
ApoI RAATTY 1 cut(s) 811
Asp700I GAANNNNTTC 1 cut(s) 386
AspA2I CCTAGG 1 cut(s) 391
AspLEI GCGC 2 cut(s) 414, 584
AspS9I GGNCC 2 cut(s) 100, 693
AsuC2I CCSGG 1 cut(s) 563
AsuHPI GGTGA 1 cut(s) 563
AvaI CYCGRG 1 cut(s) 142
AvrII CCTAGG 1 cut(s) 391
AxyI CCTNAGG 1 cut(s) 741
BanII GRGCYC 1 cut(s) 489
Bbv12I GWGCWC 1 cut(s) 489
BbvI GCAGC 3 cut(s) 17, 554, 746
BceAI ACGGC 3 cut(s) 124, 454, 595
BciT130I CCWGG 1 cut(s) 493
BcnI CCSGG 1 cut(s) 563
BfaI CTAG 4 cut(s) 23, 125, 392, 575
BfuAI ACCTGC 1 cut(s) 669
BglI GCCNNNNNGGC 1 cut(s) 108
BisI GCNGC 7 cut(s) 6, 78, 540, 543, 735, 747, 757
BlnI CCTAGG 1 cut(s) 391
BlpI GCTNAGC 1 cut(s) 257
BlsI GCNGC 7 cut(s) 7, 79, 541, 544, 736, 748, 758
BmcAI AGTACT 1 cut(s) 244
Bme1390I CCNGG 2 cut(s) 493, 563
BmeT110I CYCGRG 1 cut(s) 142
BmgT120I GGNCC 2 cut(s) 100, 693
BmiI GGNNCC 3 cut(s) 101, 121, 702
BmrFI CCNGG 2 cut(s) 493, 563
BmsI GCATC 5 cut(s) 121, 343, 556, 766, 881
BplI GAGNNNNNCTC 2 cut(s) 822, 854
BpmI CTGGAG 1 cut(s) 862
Bpu1102I GCTNAGC 1 cut(s) 257
BpuEI CTTGAG 2 cut(s) 57, 767
BpuMI CCSGG 1 cut(s) 563
BsaJI CCNNGG 3 cut(s) 78, 391, 696
BsaXI ACNNNNNCTCC 2 cut(s) 306, 336
Bsc4I CCNNNNNNNGG 3 cut(s) 421, 758, 824
Bse118I RCCGGY 3 cut(s) 218, 593, 730
Bse1I ACTGG 2 cut(s) 154, 643
Bse21I CCTNAGG 1 cut(s) 741
BseBI CCWGG 1 cut(s) 493
BseDI CCNNGG 3 cut(s) 78, 391, 696
BseGI GGATG 2 cut(s) 571, 872
BseLI CCNNNNNNNGG 3 cut(s) 421, 758, 824
BseMII CTCAG 2 cut(s) 248, 732
BseNI ACTGG 2 cut(s) 154, 643
BseRI GAGGAG 2 cut(s) 293, 843
BseXI GCAGC 3 cut(s) 17, 554, 746
BseYI CCCAGC 1 cut(s) 233
Bsh1236I CGCG 2 cut(s) 80, 412
BshFI GGCC 4 cut(s) 102, 222, 695, 746
BsiHKAI GWGCWC 1 cut(s) 489
BsiHKCI CYCGRG 1 cut(s) 142
BsiSI CCGG 4 cut(s) 219, 563, 594, 731
BslFI GGGAC 2 cut(s) 22, 437
BslI CCNNNNNNNGG 3 cut(s) 421, 758, 824
BsmFI GGGAC 2 cut(s) 22, 437
BsnI GGCC 4 cut(s) 102, 222, 695, 746
BsoBI CYCGRG 1 cut(s) 142
Bsp1286I GDGCHC 1 cut(s) 489
Bsp143I GATC 1 cut(s) 45
Bsp1720I GCTNAGC 1 cut(s) 257
BspACI CCGC 8 cut(s) 78, 80, 405, 416, 540, 747, 756, 759
BspANI GGCC 4 cut(s) 102, 222, 695, 746
BspCNI CTCAG 2 cut(s) 249, 733
BspFNI CGCG 2 cut(s) 80, 412
BspLI GGNNCC 3 cut(s) 101, 121, 702
BspMI ACCTGC 1 cut(s) 669
BspPI GGATC 1 cut(s) 40
BsrBI CCGCTC 2 cut(s) 749, 756
BsrFI RCCGGY 3 cut(s) 218, 593, 730
BsrI ACTGG 2 cut(s) 154, 643
BssAI RCCGGY 3 cut(s) 218, 593, 730
BssECI CCNNGG 3 cut(s) 78, 391, 696
BssMI GATC 1 cut(s) 45
BssT1I CCWWGG 1 cut(s) 391
Bst2UI CCWGG 1 cut(s) 493
Bst4CI ACNGT 3 cut(s) 529, 656, 863
BstC8I GCNNGC 7 cut(s) 136, 220, 277, 416, 489, 709, 848
BstDEI CTNAG 2 cut(s) 257, 741
BstDSI CCRYGG 1 cut(s) 78
BstF5I GGATG 2 cut(s) 571, 872
BstFNI CGCG 2 cut(s) 80, 412
BstHHI GCGC 2 cut(s) 414, 584
BstKTI GATC 1 cut(s) 48
BstMBI GATC 1 cut(s) 45
BstMWI GCNNNNNNNGC 6 cut(s) 86, 108, 144, 234, 353, 743
BstNI CCWGG 1 cut(s) 493
BstSCI CCNGG 2 cut(s) 491, 561
BstUI CGCG 2 cut(s) 80, 412
BstV1I GCAGC 3 cut(s) 17, 554, 746
Bsu36I CCTNAGG 1 cut(s) 741
BsuRI GGCC 4 cut(s) 102, 222, 695, 746
BtgI CCRYGG 1 cut(s) 78
BtsCI GGATG 2 cut(s) 571, 872
BtsI GCAGTG 1 cut(s) 223
BtsIMutI CAGTG 1 cut(s) 223
BveI ACCTGC 1 cut(s) 669
Cac8I GCNNGC 7 cut(s) 136, 220, 277, 416, 489, 709, 848
CaiI CAGNNNCTG 1 cut(s) 740
CfoI GCGC 2 cut(s) 414, 584
Cfr10I RCCGGY 3 cut(s) 218, 593, 730
Cfr13I GGNCC 2 cut(s) 100, 693
Cfr42I CCGCGG 1 cut(s) 81
CseI GACGC 3 cut(s) 250, 434, 674
Csp6I GTAC 2 cut(s) 243, 823
CviAII CATG 2 cut(s) 60, 639
CviQI GTAC 2 cut(s) 243, 823
DdeI CTNAG 2 cut(s) 257, 741
DpnI GATC 1 cut(s) 47
DpnII GATC 1 cut(s) 45
EaeI YGGCCR 1 cut(s) 220
EciI GGCGGA 1 cut(s) 774
Ecl136II GAGCTC 1 cut(s) 487
Eco130I CCWWGG 1 cut(s) 391
Eco24I GRGCYC 1 cut(s) 489
Eco53kI GAGCTC 1 cut(s) 487
Eco57I CTGAAG 1 cut(s) 327
Eco81I CCTNAGG 1 cut(s) 741
Eco88I CYCGRG 1 cut(s) 142
EcoICRI GAGCTC 1 cut(s) 487
EcoRI GAATTC 1 cut(s) 811
EcoRII CCWGG 1 cut(s) 491
EcoT14I CCWWGG 1 cut(s) 391
EcoT38I GRGCYC 1 cut(s) 489
ErhI CCWWGG 1 cut(s) 391
FaeI CATG 2 cut(s) 63, 642
FaqI GGGAC 2 cut(s) 22, 437
FatI CATG 2 cut(s) 59, 638
FauI CCCGC 2 cut(s) 73, 423
FblI GTMKAC 1 cut(s) 264
Fnu4HI GCNGC 7 cut(s) 6, 78, 540, 543, 735, 747, 757
FokI GGATG 2 cut(s) 578, 859
FriOI GRGCYC 1 cut(s) 489
Fsp4HI GCNGC 7 cut(s) 6, 78, 540, 543, 735, 747, 757
FspBI CTAG 4 cut(s) 23, 125, 392, 575
GlaI GCGC 2 cut(s) 413, 583
GluI GCNGC 7 cut(s) 6, 78, 540, 543, 735, 747, 757
GsaI CCCAGC 1 cut(s) 237
GsuI CTGGAG 1 cut(s) 862
HaeIII GGCC 4 cut(s) 102, 222, 695, 746
HapII CCGG 4 cut(s) 219, 563, 594, 731
HgaI GACGC 3 cut(s) 250, 434, 674
HhaI GCGC 2 cut(s) 414, 584
Hin1II CATG 2 cut(s) 63, 642
Hin6I GCGC 2 cut(s) 412, 582
HinP1I GCGC 2 cut(s) 412, 582
HincII GTYRAC 2 cut(s) 163, 265
HindII GTYRAC 2 cut(s) 163, 265
HinfI GANTC 1 cut(s) 65
HpaI GTTAAC 1 cut(s) 163
HpaII CCGG 4 cut(s) 219, 563, 594, 731
HphI GGTGA 1 cut(s) 563
Hpy166II GTNNAC 3 cut(s) 163, 265, 728
Hpy188I TCNGA 4 cut(s) 188, 272, 326, 753
Hpy188III TCNNGA 6 cut(s) 43, 178, 307, 379, 740, 841
Hpy8I GTNNAC 3 cut(s) 163, 265, 728
Hpy99I CGWCG 2 cut(s) 266, 668
HpyAV CCTTC 1 cut(s) 110
HpyCH4III ACNGT 3 cut(s) 529, 656, 863
HpyCH4IV ACGT 2 cut(s) 92, 609
HpyCH4V TGCA 6 cut(s) 89, 138, 249, 356, 650, 872
HpyF10VI GCNNNNNNNGC 6 cut(s) 86, 108, 144, 234, 353, 743
HpyF3I CTNAG 2 cut(s) 257, 741
HpySE526I ACGT 2 cut(s) 92, 609
Hsp92II CATG 2 cut(s) 63, 642
HspAI GCGC 2 cut(s) 412, 582
KroI GCCGGC 1 cut(s) 218
KroNI GCCGGC 1 cut(s) 220
KspAI GTTAAC 1 cut(s) 163
KspI CCGCGG 1 cut(s) 81
Kzo9I GATC 1 cut(s) 45
LmnI GCTCC 4 cut(s) 550, 742, 754, 843
Lsp1109I GCAGC 3 cut(s) 17, 554, 746
LweI GCATC 5 cut(s) 121, 343, 556, 766, 881
MaeI CTAG 4 cut(s) 23, 125, 392, 575
MaeII ACGT 2 cut(s) 92, 609
MaeIII GTNAC 3 cut(s) 329, 495, 786
MalI GATC 1 cut(s) 47
MbiI CCGCTC 2 cut(s) 749, 756
MboI GATC 1 cut(s) 45
MboII GAAGA 2 cut(s) 48, 788
MhlI GDGCHC 1 cut(s) 489
MluCI AATT 2 cut(s) 84, 811
MlyI GAGTC 1 cut(s) 74
MmeI TCCRAC 2 cut(s) 304, 824
MroNI GCCGGC 1 cut(s) 218
MroXI GAANNNNTTC 1 cut(s) 386
MseI TTAA 2 cut(s) 162, 807
MspA1I CMGCKG 2 cut(s) 80, 347
MspI CCGG 4 cut(s) 219, 563, 594, 731
MspR9I CCNGG 2 cut(s) 493, 563
MvaI CCWGG 1 cut(s) 493
MvnI CGCG 2 cut(s) 80, 412
MwoI GCNNNNNNNGC 6 cut(s) 86, 108, 144, 234, 353, 743
NaeI GCCGGC 1 cut(s) 220
NciI CCSGG 1 cut(s) 563
NdeII GATC 1 cut(s) 45
NgoMIV GCCGGC 1 cut(s) 218
NlaIII CATG 2 cut(s) 63, 642
NlaIV GGNNCC 3 cut(s) 101, 121, 702
NmuCI GTSAC 2 cut(s) 495, 786
PaqCI CACCTGC 1 cut(s) 669
PcsI WCGNNNNNNNCGW 1 cut(s) 660
PdiI GCCGGC 1 cut(s) 220
PdmI GAANNNNTTC 1 cut(s) 386
PfoI TCCNGGA 1 cut(s) 561
PkrI GCNGC 7 cut(s) 7, 79, 541, 544, 736, 748, 758
PleI GAGTC 1 cut(s) 73
PpsI GAGTC 1 cut(s) 73
Psp124BI GAGCTC 1 cut(s) 489
Psp6I CCWGG 1 cut(s) 491
PspFI CCCAGC 1 cut(s) 233
PspGI CCWGG 1 cut(s) 491
PspN4I GGNNCC 3 cut(s) 101, 121, 702
PspPI GGNCC 2 cut(s) 100, 693
PstNI CAGNNNCTG 1 cut(s) 740
PvuII CAGCTG 1 cut(s) 347
RsaI GTAC 2 cut(s) 244, 824
RsaNI GTAC 2 cut(s) 243, 823
SacI GAGCTC 1 cut(s) 489
SacII CCGCGG 1 cut(s) 81
SalI GTCGAC 1 cut(s) 263
SaqAI TTAA 2 cut(s) 162, 807
SatI GCNGC 7 cut(s) 6, 78, 540, 543, 735, 747, 757
Sau3AI GATC 1 cut(s) 45
Sau96I GGNCC 2 cut(s) 100, 693
ScaI AGTACT 1 cut(s) 244
SchI GAGTC 1 cut(s) 74
ScrFI CCNGG 2 cut(s) 493, 563
SduI GDGCHC 1 cut(s) 489
SfaNI GCATC 5 cut(s) 121, 343, 556, 766, 881
Sfr303I CCGCGG 1 cut(s) 81
SgrBI CCGCGG 1 cut(s) 81
SmlI CTYRAG 2 cut(s) 72, 782
SmoI CTYRAG 2 cut(s) 72, 782
Sse9I AATT 2 cut(s) 84, 811
SsiI CCGC 8 cut(s) 78, 80, 405, 416, 540, 747, 756, 759
SspMI CTAG 4 cut(s) 23, 125, 392, 575
SstI GAGCTC 1 cut(s) 489
StyD4I CCNGG 2 cut(s) 491, 561
StyI CCWWGG 1 cut(s) 391
TaaI ACNGT 3 cut(s) 529, 656, 863
TaiI ACGT 2 cut(s) 95, 612
TaqI TCGA 2 cut(s) 264, 308
TasI AATT 2 cut(s) 84, 811
TatI WGTACW 1 cut(s) 242
TauI GCSGC 4 cut(s) 80, 542, 749, 759
Tru1I TTAA 2 cut(s) 162, 807
Tru9I TTAA 2 cut(s) 162, 807
TscAI CASTG 1 cut(s) 230
TseFI GTSAC 2 cut(s) 495, 786
TseI GCWGC 3 cut(s) 5, 542, 734
Tsp45I GTSAC 2 cut(s) 495, 786
TspDTI ATGAA 1 cut(s) 48
TspGWI ACGGA 3 cut(s) 519, 528, 840
TspRI CASTG 1 cut(s) 230
XapI RAATTY 1 cut(s) 811
XmaJI CCTAGG 1 cut(s) 391
XmiI GTMKAC 1 cut(s) 264
XmnI GAANNNNTTC 1 cut(s) 386
XspI CTAG 4 cut(s) 23, 125, 392, 575
ZrmI AGTACT 1 cut(s) 244
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.