Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr2

Orthogroup ID

OG0011015

Physical Location & Seq

Genomic Coordinates Forward (+)

1135467 .. 1138670

Gene Structure 3204 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2337 bp

ATGTTCGTCCACGGAAACACCAACGGCAGCCTCAATGCGGCGGAGGACCCCGAATCCATCACCGCCCCGTTAATCTCGCGCCAGCGATCCGCCCCGAACTCCACGTCGCAGGTGGCTCTGGTGGGCGCCAATGTCTGCCCGATCGAGAGCCTCGATTACGAGATTCTCGAGAACGAGTGCTTCAAGCAGGACTGGCGGAGCCGCGGGAAGGTCCACGCGTTCCAGTACCTATTCATGAAGTGGGTCTCGTGTTTCCTCATCGGCATCGTTGTCGGACTCATTGGATTCTGCAACAACCTCGCTGTCGAAAACATTGCCGGAATTAAGTTCGTTGTCACTTCCAACATGATGTTGCAGCGCAGGTTTTGGCTGGCTTTTGCTGTCTTCTTCTTCTCGAATTTTGGTCTCACTCTGTTTGCGGTTGTGATCACGGCTTTGGTGGCGCCGGCCGCCGCGGGATCCGGTATACCGGAGGTGAAGGCTTACCTGAACGGCGTCGATGCGCCCAGAATATTTTCCATTAAGACTTTGTTTGTTAAGATTGTTGGTAGTGTTACTGCGGTGTCATCGTCTCTTCTTATCGGAAAGGCAGGGCCCATGGTGCATACCGGTGCATGTGTTGCATCATTGGTGGGTCAGGGAGGATCGAAGAAGTATGGCTTGACATGGAAATGGCTGCGATACTTCAAGAATGATCGTGACCGTAGGGATCTTGTAACGTGTGGAGCAGCTGCTGGAATTGCTGCCTCTTTCCGTTCCCCTGTCGGTGGTGTACTCTTTGCTTTTGAAGAAATGGCATCTTGGTGGAGAAGTGCACTTCTGTGGAGAGCTTTCTTTACAACTGCTCTAATTGCGATTGTGCTTCGAGCTCTGATTGATGTTTGCTACAGCGGAAAATGTGGGCTATTTGGTACTGGGGGGCTGATAATGTTTGATGTTTATTCAGCTAGTATATCATATCACATTACCGATGTGCCTCCCGTGCTCCTCCTTGGCTTTGTAGGCGGCATATTGGGCAGCCTTTACAATTCTCTATTAACAAGAGTTCTCCGAGTATACAATATCATCCATGAGAGAGGTGTCATGTATAAAATTCTTATAGCTTGCACAATCTCCGTTTTCACATCTTGTCTTCTCTTTGGATTACCATGGCTTGCATCTTGTCGACCTTGCCCACCTCATGCAGCAGAAGCCTGCCCTACGATTGGACGGTCTGGTAACTACAAGAAGTTTCAGTGCCCTCCTGGTCATTATAATGACCTTGCCAGCCTCATATTTAACACGAATGATGATGCTATTAGAAACCTCTTCAGCAAGAACACTGATTCTGAGTTTCAATATTCCTCAATTATCATATTCTTCGTCACCTGTTTTTTCTTAAGTATCTTCAGCTACGGGGTTGTTGTGCCAGCTGGTCTTTTCGTACCTGTTATTGTGACTGGTGCATCTTATGGTCGTTTTGTCGGAATGCTAGTTGGCAAACACTCGAATCTCAATCATGGTCTATATGCTGTGTTGGGTGCTGCTTCTTTTCTTGGTGGAACCATGAGGATGACGGTCTCGCTGTGTGTAATTATTCTGGAATTGACCAATAATCTGTTATTGTTGCCATTAATAATGGTGGTTCTTCTTGTTTCCAAGACAACGGCGGATGCTTTCAATGGTAATATATATGATCTTATTATGAAAGCAAAGGGTCTTCCCTATCTGGAAAGTCATGTTGAACCGTACATGAGGCAGCTGACAGTAGCGGATGTAGTAATAGGGCCACTTCAACTCTTTCAAGGCATTGAGAAGGTTGGTAACATAGTACATGTCCTCCGGACTACAGGGCATAACGGGTTCCCTGTGATCGATGAGCCTCCGTTGTCTGAAACCCCTGTTTTGTATGGTTTAGTCCTCCGCACCCATCTTATTGAATTGTTGAAGAAGAAAGCATTCGTGTCCACCCCAGTTCGGACGAGCACCAATTCTTTTAAACTCTTCTCATCGGTGGATTTTGCCAAGAGGGGTTCAGGCAACGGTGACAAGATAGAAGACATAGAATTGACTGAGGAAGAGAAGGAAATGTTCATCGACTTGCATCCCTTCACTAATGCTTCACCTTATACTGTTGTGGAGACAATGTCACTAGCAAAGGCGCTCACACTTTTCCGAGAAGTTGGCTTAAGGCACCTTCTAGTGATACCCAAGTTGTCCAATAGATCTCCCGTGGTGGGTATATTGACAAGGCACGACTTCATGCCAGAACATATACTGAGCTTACACCCTATGCTGGTAAGGAGCAGGTGGAAAAGATTAAGATTCCGGCTGCCACCTCCGTCTAAACTCTTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005216 GO:0005244 GO:0005247 GO:0005253 GO:0005254 GO:0005575 GO:0006810 GO:0006811 GO:0006820 GO:0006821 GO:0008150 GO:0008308 GO:0008509 GO:0015075 GO:0015103 GO:0015108 GO:0015267 GO:0015318 GO:0015698 GO:0016020 GO:0022803 GO:0022832 GO:0022836 GO:0022838 GO:0022839 GO:0022857 GO:0034220 GO:0051179 GO:0051234 GO:0055085 GO:0098656 GO:0098660 GO:0098661 GO:1902476

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CBS Voltage_CLC

Protein Analysis

779

Amino Acids

85.53

Weight (kDa)

9.18

Isoelectric Point (pI)

41.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Voltage_CLC	PF00654	137 - 550	6.4e-79	Voltage gated chloride channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0011015)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G33280
fragaria_vesca	FvH4_1g02460 FvH4_1g02460 FvH4_1g02460 FvH4_1g02460
malus_domestica	MD02G1021600.v1.1 MD15G1164600.v1.1
prunus_persica	Prupe.7G250100_v2.0.a1
pyrus_communis	pycom02g01900 pycom15g14760
rosa_chinensis	RchiOBHm_Chr2g0087431
rosa_laevigata	RLG00000015868
rosa_multiflora	Rmu_sc0006064.1_g000011
rosa_roxburghii	Rroxscaffold_2G00153540
rosa_rugosa	Rorug01G0475300
rosa_samantha	Rh2AG028700 Rh2BG029000 Rh2CG029000 Rh2DG028800
rosa_wichuraiana	Rw2G002290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1254
AarI	CACCTGC	2 cut(s)	100, 2277
Acc36I	ACCTGC	3 cut(s)	100, 351, 2277
AccB1I	GGYRCC	3 cut(s)	125, 442, 2172
AccI	GTMKAC	3 cut(s)	466, 1056, 1165
AccII	CGCG	4 cut(s)	79, 204, 218, 455
AccIII	TCCGGA	1 cut(s)	1821
AclWI	GGATC	5 cut(s)	81, 453, 466, 652, 717
AcoI	YGGCCR	1 cut(s)	447
AcsI	RAATTY	2 cut(s)	397, 1092
AcuI	CTGAAG	2 cut(s)	1294, 1372
AcyI	GRCGYC	3 cut(s)	126, 443, 495
AfaI	GTAC	6 cut(s)	227, 774, 913, 1425, 1730, 1812
AfiI	CCNNNNNNNGG	7 cut(s)	37, 208, 767, 1205, 1956, 2216, 2275
AflII	CTTAAG	2 cut(s)	1378, 2167
AflIII	ACRYGT	3 cut(s)	216, 719, 1813
AgeI	ACCGGT	1 cut(s)	608
AjiI	CACGTC	1 cut(s)	105
AjnI	CCWGG	1 cut(s)	1243
AleI	CACNNNNGTG	1 cut(s)	820
AluBI	AGCT	9 cut(s)	731, 830, 869, 947, 1103, 1392, 1412, 1741, 2262
AluI	AGCT	9 cut(s)	731, 830, 869, 947, 1103, 1392, 1412, 1741, 2262
Alw21I	GWGCWC	4 cut(s)	817, 871, 987, 1967
Alw26I	GTCTC	5 cut(s)	250, 410, 576, 1564, 2114
Alw44I	GTGCAC	1 cut(s)	813
AlwI	GGATC	5 cut(s)	81, 453, 466, 652, 717
AlwNI	CAGNNNCTG	1 cut(s)	734
Ama87I	CYCGRG	1 cut(s)	167
Aor13HI	TCCGGA	1 cut(s)	1821
AoxI	GGCC	3 cut(s)	447, 593, 1766
ApaI	GGGCCC	1 cut(s)	597
ApaLI	GTGCAC	1 cut(s)	813
ApoI	RAATTY	2 cut(s)	397, 1092
ArsI	GACNNNNNNTTYG	2 cut(s)	89, 121
AseI	ATTAAT	1 cut(s)	1613
AsiGI	ACCGGT	1 cut(s)	608
Asp700I	GAANNNNTTC	2 cut(s)	514, 1937
AspLEI	GCGC	6 cut(s)	81, 128, 360, 445, 505, 2143
AspS9I	GGNCC	5 cut(s)	46, 211, 593, 594, 1766
AsuHPI	GGTGA	5 cut(s)	52, 487, 1357, 2036, 2094
AvaI	CYCGRG	1 cut(s)	167
AvaII	GGWCC	2 cut(s)	46, 211
BaeGI	GKGCMC	3 cut(s)	597, 817, 1241
BaeI	ACNNNNGTAYC	2 cut(s)	2177, 2210
BamHI	GGATCC	1 cut(s)	458
BanI	GGYRCC	3 cut(s)	125, 442, 2172
BanII	GRGCYC	2 cut(s)	597, 871
BauI	CACGAG	1 cut(s)	247
BbsI	GAAGAC	4 cut(s)	376, 1124, 1691, 2043
Bbv12I	GWGCWC	4 cut(s)	817, 871, 987, 1967
BccI	CCATC	2 cut(s)	65, 1917
BceAI	ACGGC	4 cut(s)	40, 447, 508, 1662
BcgI	CGANNNNNNTGC	6 cut(s)	253, 280, 287, 296, 314, 330
BciT130I	CCWGG	1 cut(s)	1245
BclI	TGATCA	1 cut(s)	426
BcoDI	GTCTC	5 cut(s)	250, 410, 576, 1564, 2114
BfaI	CTAG	4 cut(s)	948, 1472, 2132, 2180
BfmI	CTRYAG	2 cut(s)	886, 1827
BfoI	RGCGCY	3 cut(s)	129, 446, 2144
BfrI	CTTAAG	2 cut(s)	1378, 2167
BfuAI	ACCTGC	3 cut(s)	100, 351, 2277
BglII	AGATCT	1 cut(s)	2204
Bme1390I	CCNGG	1 cut(s)	1245
Bme18I	GGWCC	2 cut(s)	46, 211
BmeT110I	CYCGRG	1 cut(s)	167
BmgBI	CACGTC	1 cut(s)	105
BmgT120I	GGNCC	5 cut(s)	46, 211, 593, 594, 1766
BmiI	GGNNCC	9 cut(s)	48, 127, 200, 444, 460, 595, 1543, 1844, 2174
BmrFI	CCNGG	1 cut(s)	1245
BmrI	ACTGGG	2 cut(s)	924, 1946
BmsI	GCATC	9 cut(s)	273, 490, 632, 806, 1166, 1282, 1454, 1642, 2092
BmuI	ACTGGG	2 cut(s)	924, 1946
BpiI	GAAGAC	4 cut(s)	376, 1124, 1691, 2043
Bsa29I	ATCGAT	1 cut(s)	1854
BsaHI	GRCGYC	3 cut(s)	126, 443, 495
BsaI	GGTCTC	3 cut(s)	250, 410, 1564
BsaJI	CCNNGG	7 cut(s)	10, 202, 453, 597, 991, 1148, 2211
BsaWI	WCCGGW	4 cut(s)	461, 469, 608, 1821
BsaXI	ACNNNNNCTCC	4 cut(s)	1803, 1833, 2111, 2141
Bsc4I	CCNNNNNNNGG	7 cut(s)	37, 208, 767, 1205, 1956, 2216, 2275
Bse118I	RCCGGY	2 cut(s)	445, 608
Bse1I	ACTGG	5 cut(s)	197, 223, 919, 1444, 1952
Bse3DI	GCAATG	1 cut(s)	312
BseAI	TCCGGA	1 cut(s)	1821
BseBI	CCWGG	1 cut(s)	1245
BseCI	ATCGAT	1 cut(s)	1854
BseDI	CCNNGG	7 cut(s)	10, 202, 453, 597, 991, 1148, 2211
BseGI	GGATG	5 cut(s)	1065, 1557, 1657, 1759, 2083
BseLI	CCNNNNNNNGG	7 cut(s)	37, 208, 767, 1205, 1956, 2216, 2275
BseMI	GCAATG	1 cut(s)	312
BseMII	CTCAG	3 cut(s)	1320, 2043, 2249
BseNI	ACTGG	5 cut(s)	197, 223, 919, 1444, 1952
BseRI	GAGGAG	1 cut(s)	977
BseSI	GKGCMC	3 cut(s)	597, 817, 1241
BseX3I	CGGCCG	1 cut(s)	447
Bsh1236I	CGCG	4 cut(s)	79, 204, 218, 455
Bsh1285I	CGRYCG	2 cut(s)	144, 450
BshFI	GGCC	3 cut(s)	449, 595, 1768
BshNI	GGYRCC	3 cut(s)	125, 442, 2172
BshTI	ACCGGT	1 cut(s)	608
BshVI	ATCGAT	1 cut(s)	1854
BsiEI	CGRYCG	2 cut(s)	144, 450
BsiHKAI	GWGCWC	4 cut(s)	817, 871, 987, 1967
BsiHKCI	CYCGRG	1 cut(s)	167
BsiSI	CCGG	7 cut(s)	318, 446, 462, 470, 609, 1822, 2308
BslI	CCNNNNNNNGG	7 cut(s)	37, 208, 767, 1205, 1956, 2216, 2275
BsmAI	GTCTC	5 cut(s)	250, 410, 576, 1564, 2114
BsmBI	CGTCTC	1 cut(s)	576
BsmI	GAATGC	2 cut(s)	1473, 1937
BsnI	GGCC	3 cut(s)	449, 595, 1768
Bso31I	GGTCTC	3 cut(s)	250, 410, 1564
BsoBI	CYCGRG	1 cut(s)	167
Bsp120I	GGGCCC	1 cut(s)	593
Bsp1286I	GDGCHC	6 cut(s)	597, 817, 871, 987, 1241, 1967
Bsp13I	TCCGGA	1 cut(s)	1821
Bsp19I	CCATGG	2 cut(s)	597, 1148
BspANI	GGCC	3 cut(s)	449, 595, 1768
BspCNI	CTCAG	3 cut(s)	1321, 2044, 2250
BspDI	ATCGAT	1 cut(s)	1854
BspEI	TCCGGA	1 cut(s)	1821
BspFNI	CGCG	4 cut(s)	79, 204, 218, 455
BspHI	TCATGA	1 cut(s)	234
BspLI	GGNNCC	9 cut(s)	48, 127, 200, 444, 460, 595, 1543, 1844, 2174
BspMI	ACCTGC	3 cut(s)	100, 351, 2277
BspPI	GGATC	5 cut(s)	81, 453, 466, 652, 717
BspT107I	GGYRCC	3 cut(s)	125, 442, 2172
BspTI	CTTAAG	2 cut(s)	1378, 2167
BspTNI	GGTCTC	3 cut(s)	250, 410, 1564
BsrDI	GCAATG	1 cut(s)	312
BsrFI	RCCGGY	2 cut(s)	445, 608
BsrI	ACTGG	5 cut(s)	197, 223, 919, 1444, 1952
BssAI	RCCGGY	2 cut(s)	445, 608
BssECI	CCNNGG	7 cut(s)	10, 202, 453, 597, 991, 1148, 2211
BssNAI	GTATAC	2 cut(s)	467, 1057
BssNI	GRCGYC	3 cut(s)	126, 443, 495
BssSI	CACGAG	1 cut(s)	247
BssT1I	CCWWGG	3 cut(s)	597, 991, 1148
Bst1107I	GTATAC	2 cut(s)	467, 1057
Bst2BI	CACGAG	1 cut(s)	247
Bst2UI	CCWGG	1 cut(s)	1245
Bst4CI	ACNGT	7 cut(s)	704, 1212, 1558, 1728, 1747, 2024, 2113
Bst6I	CTCTTC	4 cut(s)	579, 1313, 1988, 2052
BstACI	GRCGYC	3 cut(s)	126, 443, 495
BstAFI	CTTAAG	2 cut(s)	1378, 2167
BstAPI	GCANNNNNTGC	1 cut(s)	620
BstC8I	GCNNGC	8 cut(s)	83, 372, 447, 1105, 1155, 1195, 1267, 1410
BstDEI	CTNAG	3 cut(s)	1329, 2052, 2258
BstDSI	CCRYGG	6 cut(s)	10, 202, 453, 597, 1148, 2211
BstF5I	GGATG	5 cut(s)	1065, 1557, 1657, 1759, 2083
BstFNI	CGCG	4 cut(s)	79, 204, 218, 455
BstH2I	RGCGCY	3 cut(s)	129, 446, 2144
BstHHI	GCGC	6 cut(s)	81, 128, 360, 445, 505, 2143
BstMAI	GTCTC	5 cut(s)	250, 410, 576, 1564, 2114
BstMCI	CGRYCG	2 cut(s)	144, 450
BstNI	CCWGG	1 cut(s)	1245
BstNSI	RCATGY	2 cut(s)	618, 1817
BstSCI	CCNGG	1 cut(s)	1243
BstSFI	CTRYAG	2 cut(s)	886, 1827
BstSLI	GKGCMC	3 cut(s)	597, 817, 1241
BstUI	CGCG	4 cut(s)	79, 204, 218, 455
BstV2I	GAAGAC	4 cut(s)	376, 1124, 1691, 2043
BstX2I	RGATCY	3 cut(s)	458, 709, 2204
BstYI	RGATCY	3 cut(s)	458, 709, 2204
BstZ17I	GTATAC	2 cut(s)	467, 1057
BstZI	CGGCCG	1 cut(s)	447
Bsu15I	ATCGAT	1 cut(s)	1854
BsuRI	GGCC	3 cut(s)	449, 595, 1768
BsuTUI	ATCGAT	1 cut(s)	1854
BtgI	CCRYGG	6 cut(s)	10, 202, 453, 597, 1148, 2211
BtrI	CACGTC	1 cut(s)	105
BtsCI	GGATG	5 cut(s)	1065, 1557, 1657, 1759, 2083
BtsIMutI	CAGTG	2 cut(s)	1241, 1320
BveI	ACCTGC	3 cut(s)	100, 351, 2277
Cac8I	GCNNGC	8 cut(s)	83, 372, 447, 1105, 1155, 1195, 1267, 1410
CaiI	CAGNNNCTG	1 cut(s)	734
CciI	TCATGA	1 cut(s)	234
CfoI	GCGC	6 cut(s)	81, 128, 360, 445, 505, 2143
Cfr10I	RCCGGY	2 cut(s)	445, 608
Cfr13I	GGNCC	5 cut(s)	46, 211, 593, 594, 1766
Cfr42I	CCGCGG	2 cut(s)	205, 456
ClaI	ATCGAT	1 cut(s)	1854
CseI	GACGC	1 cut(s)	484
Csp6I	GTAC	6 cut(s)	226, 773, 912, 1424, 1729, 1811
CspAI	ACCGGT	1 cut(s)	608
CviQI	GTAC	6 cut(s)	226, 773, 912, 1424, 1729, 1811
DdeI	CTNAG	3 cut(s)	1329, 2052, 2258
DinI	GGCGCC	2 cut(s)	127, 444
DraI	TTTAAA	1 cut(s)	1978
EaeI	YGGCCR	1 cut(s)	447
EagI	CGGCCG	1 cut(s)	447
Eam1104I	CTCTTC	4 cut(s)	579, 1313, 1988, 2052
EarI	CTCTTC	4 cut(s)	579, 1313, 1988, 2052
EciI	GGCGGA	4 cut(s)	56, 79, 211, 1664
Ecl136II	GAGCTC	1 cut(s)	869
EclXI	CGGCCG	1 cut(s)	447
Eco130I	CCWWGG	3 cut(s)	597, 991, 1148
Eco24I	GRGCYC	2 cut(s)	597, 871
Eco31I	GGTCTC	3 cut(s)	250, 410, 1564
Eco47I	GGWCC	2 cut(s)	46, 211
Eco52I	CGGCCG	1 cut(s)	447
Eco53kI	GAGCTC	1 cut(s)	869
Eco57I	CTGAAG	2 cut(s)	1294, 1372
Eco88I	CYCGRG	1 cut(s)	167
EcoICRI	GAGCTC	1 cut(s)	869
EcoO109I	RGGNCCY	2 cut(s)	46, 593
EcoRII	CCWGG	1 cut(s)	1243
EcoT14I	CCWWGG	3 cut(s)	597, 991, 1148
EcoT38I	GRGCYC	2 cut(s)	597, 871
EgeI	GGCGCC	2 cut(s)	127, 444
EheI	GGCGCC	2 cut(s)	127, 444
ErhI	CCWWGG	3 cut(s)	597, 991, 1148
Esp3I	CGTCTC	1 cut(s)	576
FalI	AAGNNNNNCTT	2 cut(s)	644, 676
FauI	CCCGC	2 cut(s)	197, 448
FbaI	TGATCA	1 cut(s)	426
FblI	GTMKAC	3 cut(s)	466, 1056, 1165
FokI	GGATG	5 cut(s)	1052, 1564, 1664, 1766, 2070
FriOI	GRGCYC	2 cut(s)	597, 871
FspBI	CTAG	4 cut(s)	948, 1472, 2132, 2180
GlaI	GCGC	6 cut(s)	80, 127, 359, 444, 504, 2142
HaeII	RGCGCY	3 cut(s)	129, 446, 2144
HaeIII	GGCC	3 cut(s)	449, 595, 1768
HapII	CCGG	7 cut(s)	318, 446, 462, 470, 609, 1822, 2308
HgaI	GACGC	1 cut(s)	484
HhaI	GCGC	6 cut(s)	81, 128, 360, 445, 505, 2143
Hin1I	GRCGYC	3 cut(s)	126, 443, 495
Hin6I	GCGC	6 cut(s)	79, 126, 358, 443, 503, 2141
HinP1I	GCGC	6 cut(s)	79, 126, 358, 443, 503, 2141
HincII	GTYRAC	1 cut(s)	1166
HindII	GTYRAC	1 cut(s)	1166
HinfI	GANTC	7 cut(s)	53, 163, 276, 285, 1325, 1489, 2304
HpaII	CCGG	7 cut(s)	318, 446, 462, 470, 609, 1822, 2308
HphI	GGTGA	5 cut(s)	52, 487, 1357, 2036, 2094
Hpy166II	GTNNAC	8 cut(s)	10, 214, 467, 773, 815, 1057, 1166, 1947
Hpy188I	TCNGA	9 cut(s)	275, 584, 873, 1052, 1330, 1466, 1873, 1959, 2156
Hpy8I	GTNNAC	8 cut(s)	10, 214, 467, 773, 815, 1057, 1166, 1947
Hpy99I	CGWCG	2 cut(s)	109, 500
HpyAV	CCTTC	6 cut(s)	202, 472, 1789, 2056, 2098, 2186
HpyCH4III	ACNGT	7 cut(s)	704, 1212, 1558, 1728, 1747, 2024, 2113
HpyCH4IV	ACGT	2 cut(s)	104, 719
HpyF3I	CTNAG	3 cut(s)	1329, 2052, 2258
HpySE526I	ACGT	2 cut(s)	104, 719
Hsp92I	GRCGYC	3 cut(s)	126, 443, 495
HspAI	GCGC	6 cut(s)	79, 126, 358, 443, 503, 2141
KasI	GGCGCC	2 cut(s)	125, 442
Kpn2I	TCCGGA	1 cut(s)	1821
KroI	GCCGGC	1 cut(s)	445
KroNI	GCCGGC	1 cut(s)	447
Ksp22I	TGATCA	1 cut(s)	426
KspI	CCGCGG	2 cut(s)	205, 456
LmnI	GCTCC	4 cut(s)	198, 725, 990, 2283
LweI	GCATC	9 cut(s)	273, 490, 632, 806, 1166, 1282, 1454, 1642, 2092
MaeI	CTAG	4 cut(s)	948, 1472, 2132, 2180
MaeII	ACGT	2 cut(s)	104, 719
MflI	RGATCY	3 cut(s)	458, 709, 2204
MhlI	GDGCHC	6 cut(s)	597, 817, 871, 987, 1241, 1967
MluI	ACGCGT	1 cut(s)	216
Mly113I	GGCGCC	2 cut(s)	126, 443
MlyI	GAGTC	1 cut(s)	270
MmeI	TCCRAC	3 cut(s)	253, 366, 1444
MroI	TCCGGA	1 cut(s)	1821
MroNI	GCCGGC	1 cut(s)	445
MroXI	GAANNNNTTC	2 cut(s)	514, 1937
MslI	CAYNNNNRTG	6 cut(s)	609, 670, 802, 820, 1254, 1550
MspA1I	CMGCKG	6 cut(s)	204, 455, 731, 891, 1412, 1741
MspCI	CTTAAG	2 cut(s)	1378, 2167
MspI	CCGG	7 cut(s)	318, 446, 462, 470, 609, 1822, 2308
MspR9I	CCNGG	1 cut(s)	1245
Mva1269I	GAATGC	2 cut(s)	1473, 1937
MvaI	CCWGG	1 cut(s)	1245
MvnI	CGCG	4 cut(s)	79, 204, 218, 455
NaeI	GCCGGC	1 cut(s)	447
NarI	GGCGCC	2 cut(s)	126, 443
NcoI	CCATGG	2 cut(s)	597, 1148
NgoMIV	GCCGGC	1 cut(s)	445
NlaIV	GGNNCC	9 cut(s)	48, 127, 200, 444, 460, 595, 1543, 1844, 2174
NmuCI	GTSAC	6 cut(s)	334, 698, 1363, 1435, 2024, 2127
NspI	RCATGY	2 cut(s)	618, 1817
OliI	CACNNNNGTG	1 cut(s)	820
PaeR7I	CTCGAG	1 cut(s)	167
PagI	TCATGA	1 cut(s)	234
PaqCI	CACCTGC	2 cut(s)	100, 2277
PciI	ACATGT	1 cut(s)	1813
PcsI	WCGNNNNNNNCGW	2 cut(s)	150, 165
PctI	GAATGC	2 cut(s)	1473, 1937
PdiI	GCCGGC	1 cut(s)	447
PdmI	GAANNNNTTC	2 cut(s)	514, 1937
PfeI	GAWTC	6 cut(s)	53, 163, 285, 1325, 1489, 2304
PflFI	GACNNNGTC	1 cut(s)	2125
PinAI	ACCGGT	1 cut(s)	608
Ple19I	CGATCG	1 cut(s)	144
PleI	GAGTC	1 cut(s)	270
PluTI	GGCGCC	2 cut(s)	129, 446
PpsI	GAGTC	1 cut(s)	270
PpuMI	RGGWCCY	1 cut(s)	46
PscI	ACATGT	1 cut(s)	1813
PshBI	ATTAAT	1 cut(s)	1613
PsiI	TTATAA	1 cut(s)	1254
Psp124BI	GAGCTC	1 cut(s)	871
Psp5II	RGGWCCY	1 cut(s)	46
Psp6I	CCWGG	1 cut(s)	1243
PspGI	CCWGG	1 cut(s)	1243
PspN4I	GGNNCC	9 cut(s)	48, 127, 200, 444, 460, 595, 1543, 1844, 2174
PspOMI	GGGCCC	1 cut(s)	593
PspPI	GGNCC	5 cut(s)	46, 211, 593, 594, 1766
PspPPI	RGGWCCY	1 cut(s)	46
PstNI	CAGNNNCTG	1 cut(s)	734
PsuI	RGATCY	3 cut(s)	458, 709, 2204
PsyI	GACNNNGTC	1 cut(s)	2125
PvuI	CGATCG	1 cut(s)	144
PvuII	CAGCTG	3 cut(s)	731, 1412, 1741
RsaI	GTAC	6 cut(s)	227, 774, 913, 1425, 1730, 1812
RsaNI	GTAC	6 cut(s)	226, 773, 912, 1424, 1729, 1811
RseI	CAYNNNNRTG	6 cut(s)	609, 670, 802, 820, 1254, 1550
SacI	GAGCTC	1 cut(s)	871
SacII	CCGCGG	2 cut(s)	205, 456
SalI	GTCGAC	1 cut(s)	1164
Sau96I	GGNCC	5 cut(s)	46, 211, 593, 594, 1766
SchI	GAGTC	1 cut(s)	270
ScrFI	CCNGG	1 cut(s)	1245
SduI	GDGCHC	6 cut(s)	597, 817, 871, 987, 1241, 1967
SfaNI	GCATC	9 cut(s)	273, 490, 632, 806, 1166, 1282, 1454, 1642, 2092
SfcI	CTRYAG	2 cut(s)	886, 1827
SfoI	GGCGCC	2 cut(s)	127, 444
Sfr274I	CTCGAG	1 cut(s)	167
Sfr303I	CCGCGG	2 cut(s)	205, 456
SgrBI	CCGCGG	2 cut(s)	205, 456
SinI	GGWCC	2 cut(s)	46, 211
SlaI	CTCGAG	1 cut(s)	167
SmiMI	CAYNNNNRTG	6 cut(s)	609, 670, 802, 820, 1254, 1550
SmlI	CTYRAG	3 cut(s)	167, 1378, 2167
SmoI	CTYRAG	3 cut(s)	167, 1378, 2167
SspDI	GGCGCC	2 cut(s)	125, 442
SspI	AATATT	2 cut(s)	513, 1340
SspMI	CTAG	4 cut(s)	948, 1472, 2132, 2180
SstI	GAGCTC	1 cut(s)	871
StyD4I	CCNGG	1 cut(s)	1243
StyI	CCWWGG	3 cut(s)	597, 991, 1148
TaaI	ACNGT	7 cut(s)	704, 1212, 1558, 1728, 1747, 2024, 2113
TaiI	ACGT	2 cut(s)	107, 722
TatI	WGTACW	2 cut(s)	772, 1810
TauI	GCSGC	5 cut(s)	41, 204, 452, 455, 1008
TfiI	GAWTC	6 cut(s)	53, 163, 285, 1325, 1489, 2304
TscAI	CASTG	2 cut(s)	1241, 1327
TseFI	GTSAC	6 cut(s)	334, 698, 1363, 1435, 2024, 2127
Tsp45I	GTSAC	6 cut(s)	334, 698, 1363, 1435, 2024, 2127
TspDTI	ATGAA	5 cut(s)	223, 251, 1700, 2062, 2230
TspGWI	ACGGA	5 cut(s)	27, 743, 1105, 1854, 2310
TspRI	CASTG	2 cut(s)	1241, 1327
Tth111I	GACNNNGTC	1 cut(s)	2125
Vha464I	CTTAAG	2 cut(s)	1378, 2167
VneI	GTGCAC	1 cut(s)	813
VpaK11BI	GGWCC	2 cut(s)	46, 211
VspI	ATTAAT	1 cut(s)	1613
XapI	RAATTY	2 cut(s)	397, 1092
XceI	RCATGY	2 cut(s)	618, 1817
XcmI	CCANNNNNNNNNTGG	1 cut(s)	109
XhoI	CTCGAG	1 cut(s)	167
XmiI	GTMKAC	3 cut(s)	466, 1056, 1165
XmnI	GAANNNNTTC	2 cut(s)	514, 1937
XspI	CTAG	4 cut(s)	948, 1472, 2132, 2180

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.