pycom15g14760

chloride

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0011015
Physical Location & Seq
Forward (+)
10116152 .. 10119369
3218 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g14760.2

Sequence Viewer

Length: 2337 bp
ATGTTCATCAACGGCAACACCAACGCCAGCCTCAATGCGGCGGAGGACCCGGAATCCATCACCGCCCCGTTAATCTCGCGCCAGCGGTACGCCCCGAACTCCACGTCGCAGGTGGCGCTGGTCGGCGCCAAAGTCCGCCCAATCGAGAGCCTCGATTACGAGATTCTCGAGAACGAGTTCTTCAAGCAGGACTGGCGGAGTTGCGGGAAGGCCCACGTGTTACAGTACATATTCATGAAGTGGGTCTCGTGTTTCCTTGTTGGCATCGTCGTCGGACTCATTGCATTCTGCAACAACCTCGCCGTCGAAAACATCGCCGGCATTAAGTTTGTTGTCACGTCCAACATGATGTTGCAGCGCAGGTTTTGGCTGGCTTTTGGCGTCTTCTTCTTCTCAAATTTGGGTTTGACTTTATTCGCGGTTGTGGTCACCGCTTTCGTGGCGCCCGCCGCCGCAGGGTCCGGTATACCGGAGGTGAAGGCGTACCTGAACGGCGTCGATGCGCCCGGAATATATTCAATAAAGACTTTGCTTGTTAAGATTGTTGGCAGTGTTACTGCAGTGTCGTCCTCTCTTCTTATTGGAAAGGCAGGGCCCATGGTGCATATCGGTGCGTGTGTTGCATCATTGTTGGGTCAGGGAGGATCGAAGAAGCATGGCTTGACATGGAAATGGCTGCGATATTTCAAGAATGATCGCGATCGTAGGGATCTCGTAACGTGTGGAGCAGCTGCTGGAATTGGTGCATCCTTTCGTTCCCCTGTCGGTGGGGTACTCTTTGTTTTTGAAGAAATGGCATCTTGGTGGAGAAGTGCCCTTCTGTGGAGAGCTTTCTTTACAACAGCTGTAATCGCAATTGTGCTTCGAGCTCTGATTGATGTTTGCTACAGCGGAAAATGTGGGCTTTTTGGTACTGGGGGGCTGATAATGTTTGATGTCTATTCAGCAAATATATCATATCACATTACCGATGTGCCTCCGGTGCTTCTCCTTGGGTTTGTAGGCGGCATATTGGGCAGCCTATACAATTCTCTATTAACAAAAGTTATCCGAATATACAATATCATCCATGAGAGAGGTGTCGTTTATAAAATTCTTATAGCTTGTACAGTCTCCATTTTCACATCTTGTCTTCTTTTTGGATTACCGTGGCTTGCAACTTGTCGACCTTGCCCACGTGATGCAGTCGAAGCCTGCCCTACCATTGGACGGTCTGGTAACTACAAGAAGTTTCAATGTCCTGCTGGTCACTATAATGACCTTGCCAGCCTCATATTTAACACGAATGATGATGCTATTAGAAACCTCTTCAGCAAGAATACTGATTCTGAGTTTCAATATTCCTCGATGATCATATTTTTTGTCACCTGTTTTTTCTTGAGTATCTTTAGCTACGGGGTGGTTGTGCCAGCTGGTCTTTTCGTACCTGTTATTGTGACTGGTGCATCTTATGGGCGTTTTTTTGGAATGCTGGTTGGTAAACACTCGAATCTCAATCACGGTCTATATGCTGTGCTGGGTGCTGCTGCTCTTCTTGGTGGAACCATGAGGATGACGGTCTCTCTATGTGTAATTATTCTGGAATTGACCAATAATCTGTTATTGCTGCCATTAATAATGGTGGTTCTTCTTGTTTCAAAGACAACGGCAGATGCTTTCAATAGCAATATATATGATCTTATTATGAAAGCAAAGGGTCTTCCCTATCTGGAAAGTCATGTTGAACCATATATGCGGCAGCTGACAGTAGCAGATGTGGTAACAGGGCCACTTCAACTATTTCAAGGCATTGAGAAGGTTGGTAACATAGTACACGTCCTCCGTACTACAGGGCATAATGGGTTCCCTGTGATTGATGAGCCTCCGCTTTCTGAAACGCCTGTTTTGTTTGGTATAGTCCTCCGTGCCCATCTTATTGAATTGCTGAAGAAGAAAGCATTTGTGTCCAACCCAGTTCTTGCGAGCACCGATTCTTTTAAACTCTTCTCATCAGTGGATTTTGCCAAGAGGGGTTCAGGCAAAGGGGACAAGATAGAAGATATAGAACTAACCGAGGAAGAGATGGAAATGTTCATCGACTTGCATCCCTTTACTAATACTTCACCTTATACTGTTGTGGAAACGATGTCACTGGCAAAGGCTCTTATACTTTTCCGAGAAGTTGGCTTAAGGCACCTTCTTGTGATACCCAAGCTGTCCAATAGAACTCCCGTGGTGGGTATATTGACGAGGCACGACTTCATGCCAGAACATATATTGAGCTTGCACCCAATGCTGGTGAGTAGCAGGTGGAAAAGATTAAGATTCCAGATGCCATCTCCGCCAAAACTCATTTAG

Protein Analysis

779

Amino Acids

85.41

Weight (kDa)

9.02

Isoelectric Point (pI)

41.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011015)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G33280
fragaria_vesca FvH4_1g02460 FvH4_1g02460 FvH4_1g02460 FvH4_1g02460
malus_domestica MD02G1021600.v1.1 MD15G1164600.v1.1
prunus_persica Prupe.7G250100_v2.0.a1
pyrus_communis pycom02g01900 pycom15g14760
rosa_chinensis RchiOBHm_Chr2g0087431
rosa_laevigata RLG00000015868
rosa_multiflora Rmu_sc0006064.1_g000011
rosa_roxburghii Rroxscaffold_2G00153540
rosa_rugosa Rorug01G0475300
rosa_samantha Rh2AG028700 Rh2BG029000 Rh2CG029000 Rh2DG028800
rosa_wichuraiana Rw2G002290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1089
AarI CACCTGC 2 cut(s) 100, 2277
Acc36I ACCTGC 3 cut(s) 100, 351, 2277
AccB1I GGYRCC 3 cut(s) 125, 442, 2172
AccI GTMKAC 2 cut(s) 466, 1165
AccII CGCG 3 cut(s) 79, 419, 699
AclWI GGATC 2 cut(s) 652, 717
AcsI RAATTY 2 cut(s) 397, 1092
AcuI CTGAAG 2 cut(s) 1294, 1946
AcvI CACGTG 2 cut(s) 217, 1178
AcyI GRCGYC 4 cut(s) 126, 381, 443, 495
AfaI GTAC 9 cut(s) 89, 227, 485, 774, 913, 1108, 1425, 1812, 1825
AfiI CCNNNNNNNGG 8 cut(s) 37, 456, 767, 822, 1205, 1209, 2216, 2275
AflII CTTAAG 1 cut(s) 2167
AflIII ACRYGT 3 cut(s) 216, 719, 1813
AjiI CACGTC 3 cut(s) 105, 339, 1816
Alw21I GWGCWC 2 cut(s) 871, 1967
Alw26I GTCTC 3 cut(s) 250, 1117, 1564
AlwI GGATC 2 cut(s) 652, 717
AlwNI CAGNNNCTG 1 cut(s) 734
Ama87I CYCGRG 1 cut(s) 167
AoxI GGCC 3 cut(s) 210, 593, 1766
ApaI GGGCCC 1 cut(s) 597
ApeKI GCWGC 9 cut(s) 355, 676, 728, 731, 1017, 1523, 1526, 1606, 1738
ApoI RAATTY 2 cut(s) 397, 1092
ArsI GACNNNNNNTTYG 2 cut(s) 89, 121
AseI ATTAAT 1 cut(s) 1613
Asp700I GAANNNNTTC 2 cut(s) 176, 514
AspLEI GCGC 6 cut(s) 81, 118, 128, 360, 445, 505
AspS9I GGNCC 6 cut(s) 46, 211, 459, 593, 594, 1766
AsuC2I CCSGG 2 cut(s) 50, 507
AsuHPI GGTGA 6 cut(s) 52, 421, 487, 1357, 2094, 2290
AvaI CYCGRG 1 cut(s) 167
AvaII GGWCC 2 cut(s) 46, 459
BaeGI GKGCMC 3 cut(s) 597, 817, 1909
BanI GGYRCC 3 cut(s) 125, 442, 2172
BanII GRGCYC 2 cut(s) 597, 871
BauI CACGAG 1 cut(s) 247
BbrPI CACGTG 2 cut(s) 217, 1178
BbsI GAAGAC 3 cut(s) 376, 1124, 1691
Bbv12I GWGCWC 2 cut(s) 871, 1967
BbvI GCAGC 9 cut(s) 367, 663, 718, 740, 1029, 1510, 1513, 1593, 1750
BccI CCATC 4 cut(s) 65, 1917, 2056, 2323
BceAI ACGGC 4 cut(s) 28, 287, 508, 1662
BcgI CGANNNNNNTGC 4 cut(s) 253, 280, 287, 314
BclI TGATCA 1 cut(s) 1350
BcnI CCSGG 2 cut(s) 50, 507
BcoDI GTCTC 3 cut(s) 250, 1117, 1564
BfmI CTRYAG 3 cut(s) 558, 886, 1827
BfoI RGCGCY 3 cut(s) 119, 129, 446
BfrI CTTAAG 1 cut(s) 2167
BfuAI ACCTGC 3 cut(s) 100, 351, 2277
Bme1390I CCNGG 2 cut(s) 50, 507
Bme18I GGWCC 2 cut(s) 46, 459
BmeT110I CYCGRG 1 cut(s) 167
BmgBI CACGTC 3 cut(s) 105, 339, 1816
BmgT120I GGNCC 6 cut(s) 46, 211, 459, 593, 594, 1766
BmiI GGNNCC 8 cut(s) 48, 127, 444, 460, 595, 1543, 1844, 2174
BmrFI CCNGG 2 cut(s) 50, 507
BmrI ACTGGG 2 cut(s) 924, 1946
BmuI ACTGGG 2 cut(s) 924, 1946
BpiI GAAGAC 3 cut(s) 376, 1124, 1691
BpuEI CTTGAG 1 cut(s) 1399
BpuMI CCSGG 2 cut(s) 50, 507
BsaAI YACGTR 2 cut(s) 217, 1178
BsaBI GATNNNNATC 1 cut(s) 699
BsaHI GRCGYC 4 cut(s) 126, 381, 443, 495
BsaI GGTCTC 2 cut(s) 250, 1564
BsaJI CCNNGG 5 cut(s) 597, 991, 1148, 2052, 2211
BsaWI WCCGGW 3 cut(s) 461, 469, 979
BsaXI ACNNNNNCTCC 2 cut(s) 1803, 1833
Bsc4I CCNNNNNNNGG 8 cut(s) 37, 456, 767, 822, 1205, 1209, 2216, 2275
Bse118I RCCGGY 1 cut(s) 317
Bse1I ACTGG 5 cut(s) 197, 919, 1444, 1952, 2136
Bse3DI GCAATG 1 cut(s) 279
Bse8I GATNNNNATC 1 cut(s) 699
BseDI CCNNGG 5 cut(s) 597, 991, 1148, 2052, 2211
BseGI GGATG 4 cut(s) 746, 1065, 1557, 2083
BseJI GATNNNNATC 1 cut(s) 699
BseLI CCNNNNNNNGG 8 cut(s) 37, 456, 767, 822, 1205, 1209, 2216, 2275
BseMI GCAATG 1 cut(s) 279
BseMII CTCAG 1 cut(s) 1320
BseNI ACTGG 5 cut(s) 197, 919, 1444, 1952, 2136
BseSI GKGCMC 3 cut(s) 597, 817, 1909
BseXI GCAGC 9 cut(s) 367, 663, 718, 740, 1029, 1510, 1513, 1593, 1750
BseYI CCCAGC 1 cut(s) 1516
Bsh1236I CGCG 3 cut(s) 79, 419, 699
Bsh1285I CGRYCG 1 cut(s) 703
BshFI GGCC 3 cut(s) 212, 595, 1768
BshNI GGYRCC 3 cut(s) 125, 442, 2172
BsiEI CGRYCG 1 cut(s) 703
BsiHKAI GWGCWC 2 cut(s) 871, 1967
BsiHKCI CYCGRG 1 cut(s) 167
BsiSI CCGG 6 cut(s) 50, 318, 462, 470, 507, 980
BslFI GGGAC 1 cut(s) 2039
BslI CCNNNNNNNGG 8 cut(s) 37, 456, 767, 822, 1205, 1209, 2216, 2275
BsmAI GTCTC 3 cut(s) 250, 1117, 1564
BsmFI GGGAC 1 cut(s) 2039
BsmI GAATGC 2 cut(s) 284, 1473
BsnI GGCC 3 cut(s) 212, 595, 1768
Bso31I GGTCTC 2 cut(s) 250, 1564
BsoBI CYCGRG 1 cut(s) 167
Bsp120I GGGCCC 1 cut(s) 593
Bsp1286I GDGCHC 5 cut(s) 597, 817, 871, 1909, 1967
Bsp1407I TGTACA 1 cut(s) 1106
Bsp143I GATC 6 cut(s) 644, 694, 700, 709, 1350, 1675
Bsp19I CCATGG 1 cut(s) 597
Bsp68I TCGCGA 1 cut(s) 699
BspANI GGCC 3 cut(s) 212, 595, 1768
BspCNI CTCAG 1 cut(s) 1321
BspFNI CGCG 3 cut(s) 79, 419, 699
BspHI TCATGA 1 cut(s) 234
BspLI GGNNCC 8 cut(s) 48, 127, 444, 460, 595, 1543, 1844, 2174
BspMAI CTGCAG 1 cut(s) 562
BspMI ACCTGC 3 cut(s) 100, 351, 2277
BspPI GGATC 2 cut(s) 652, 717
BspQI GCTCTTC 1 cut(s) 1536
BspT107I GGYRCC 3 cut(s) 125, 442, 2172
BspTI CTTAAG 1 cut(s) 2167
BspTNI GGTCTC 2 cut(s) 250, 1564
BsrDI GCAATG 1 cut(s) 279
BsrFI RCCGGY 1 cut(s) 317
BsrGI TGTACA 1 cut(s) 1106
BsrI ACTGG 5 cut(s) 197, 919, 1444, 1952, 2136
BssAI RCCGGY 1 cut(s) 317
BssECI CCNNGG 5 cut(s) 597, 991, 1148, 2052, 2211
BssMI GATC 6 cut(s) 644, 694, 700, 709, 1350, 1675
BssNAI GTATAC 1 cut(s) 467
BssNI GRCGYC 4 cut(s) 126, 381, 443, 495
BssSI CACGAG 1 cut(s) 247
BssT1I CCWWGG 2 cut(s) 597, 991
Bst1107I GTATAC 1 cut(s) 467
Bst2BI CACGAG 1 cut(s) 247
Bst4CI ACNGT 8 cut(s) 225, 1111, 1149, 1212, 1502, 1558, 1747, 2113
Bst6I CTCTTC 5 cut(s) 579, 1313, 1536, 1988, 2052
BstACI GRCGYC 4 cut(s) 126, 381, 443, 495
BstAFI CTTAAG 1 cut(s) 2167
BstAPI GCANNNNNTGC 1 cut(s) 2272
BstAUI TGTACA 1 cut(s) 1106
BstBAI YACGTR 2 cut(s) 217, 1178
BstDEI CTNAG 1 cut(s) 1329
BstDSI CCRYGG 3 cut(s) 597, 1148, 2211
BstEII GGTNACC 1 cut(s) 427
BstF5I GGATG 4 cut(s) 746, 1065, 1557, 2083
BstFNI CGCG 3 cut(s) 79, 419, 699
BstH2I RGCGCY 3 cut(s) 119, 129, 446
BstHHI GCGC 6 cut(s) 81, 118, 128, 360, 445, 505
BstKTI GATC 6 cut(s) 647, 697, 703, 712, 1353, 1678
BstMAI GTCTC 3 cut(s) 250, 1117, 1564
BstMBI GATC 6 cut(s) 644, 694, 700, 709, 1350, 1675
BstMCI CGRYCG 1 cut(s) 703
BstPI GGTNACC 1 cut(s) 427
BstSCI CCNGG 2 cut(s) 48, 505
BstSFI CTRYAG 3 cut(s) 558, 886, 1827
BstSLI GKGCMC 3 cut(s) 597, 817, 1909
BstUI CGCG 3 cut(s) 79, 419, 699
BstV1I GCAGC 9 cut(s) 367, 663, 718, 740, 1029, 1510, 1513, 1593, 1750
BstV2I GAAGAC 3 cut(s) 376, 1124, 1691
BstX2I RGATCY 1 cut(s) 709
BstYI RGATCY 1 cut(s) 709
BstZ17I GTATAC 1 cut(s) 467
BsuRI GGCC 3 cut(s) 212, 595, 1768
BtgI CCRYGG 3 cut(s) 597, 1148, 2211
BtgZI GCGATG 1 cut(s) 298
BtrI CACGTC 3 cut(s) 105, 339, 1816
BtsCI GGATG 4 cut(s) 746, 1065, 1557, 2083
BtsI GCAGTG 2 cut(s) 556, 567
BtsIMutI CAGTG 4 cut(s) 556, 567, 1998, 2129
BtuMI TCGCGA 1 cut(s) 699
BveI ACCTGC 3 cut(s) 100, 351, 2277
CaiI CAGNNNCTG 1 cut(s) 734
CciI TCATGA 1 cut(s) 234
CfoI GCGC 6 cut(s) 81, 118, 128, 360, 445, 505
Cfr10I RCCGGY 1 cut(s) 317
Cfr13I GGNCC 6 cut(s) 46, 211, 459, 593, 594, 1766
CseI GACGC 2 cut(s) 370, 484
Csp6I GTAC 9 cut(s) 88, 226, 484, 773, 912, 1107, 1424, 1811, 1824
CviAII CATG 9 cut(s) 235, 346, 598, 656, 666, 1070, 1546, 1718, 2242
CviQI GTAC 9 cut(s) 88, 226, 484, 773, 912, 1107, 1424, 1811, 1824
DdeI CTNAG 1 cut(s) 1329
DinI GGCGCC 2 cut(s) 127, 444
DpnI GATC 6 cut(s) 646, 696, 702, 711, 1352, 1677
DpnII GATC 6 cut(s) 644, 694, 700, 709, 1350, 1675
DraI TTTAAA 1 cut(s) 1978
Eam1104I CTCTTC 5 cut(s) 579, 1313, 1536, 1988, 2052
EarI CTCTTC 5 cut(s) 579, 1313, 1536, 1988, 2052
EciI GGCGGA 4 cut(s) 56, 125, 211, 2310
Ecl136II GAGCTC 1 cut(s) 869
Eco130I CCWWGG 2 cut(s) 597, 991
Eco24I GRGCYC 2 cut(s) 597, 871
Eco31I GGTCTC 2 cut(s) 250, 1564
Eco47I GGWCC 2 cut(s) 46, 459
Eco53kI GAGCTC 1 cut(s) 869
Eco57I CTGAAG 2 cut(s) 1294, 1946
Eco72I CACGTG 2 cut(s) 217, 1178
Eco88I CYCGRG 1 cut(s) 167
Eco91I GGTNACC 1 cut(s) 427
EcoICRI GAGCTC 1 cut(s) 869
EcoO109I RGGNCCY 2 cut(s) 46, 593
EcoO65I GGTNACC 1 cut(s) 427
EcoT14I CCWWGG 2 cut(s) 597, 991
EcoT38I GRGCYC 2 cut(s) 597, 871
EgeI GGCGCC 2 cut(s) 127, 444
EheI GGCGCC 2 cut(s) 127, 444
ErhI CCWWGG 2 cut(s) 597, 991
FaeI CATG 9 cut(s) 238, 349, 601, 659, 669, 1073, 1549, 1721, 2245
FalI AAGNNNNNCTT 2 cut(s) 644, 676
FaqI GGGAC 1 cut(s) 2039
FatI CATG 9 cut(s) 234, 345, 597, 655, 665, 1069, 1545, 1717, 2241
FauI CCCGC 2 cut(s) 197, 454
FbaI TGATCA 1 cut(s) 1350
FblI GTMKAC 2 cut(s) 466, 1165
FokI GGATG 4 cut(s) 733, 1052, 1564, 2070
FriOI GRGCYC 2 cut(s) 597, 871
GlaI GCGC 6 cut(s) 80, 117, 127, 359, 444, 504
GsaI CCCAGC 1 cut(s) 1520
HaeII RGCGCY 3 cut(s) 119, 129, 446
HaeIII GGCC 3 cut(s) 212, 595, 1768
HapII CCGG 6 cut(s) 50, 318, 462, 470, 507, 980
HgaI GACGC 2 cut(s) 370, 484
HhaI GCGC 6 cut(s) 81, 118, 128, 360, 445, 505
Hin1I GRCGYC 4 cut(s) 126, 381, 443, 495
Hin1II CATG 9 cut(s) 238, 349, 601, 659, 669, 1073, 1549, 1721, 2245
Hin6I GCGC 6 cut(s) 79, 116, 126, 358, 443, 503
HinP1I GCGC 6 cut(s) 79, 116, 126, 358, 443, 503
HincII GTYRAC 1 cut(s) 1166
HindII GTYRAC 1 cut(s) 1166
HinfI GANTC 7 cut(s) 53, 163, 276, 1325, 1489, 1970, 2304
HpaII CCGG 6 cut(s) 50, 318, 462, 470, 507, 980
HphI GGTGA 6 cut(s) 52, 421, 487, 1357, 2094, 2290
Hpy166II GTNNAC 4 cut(s) 467, 1166, 1481, 1813
Hpy188I TCNGA 6 cut(s) 275, 873, 1052, 1330, 1873, 2156
Hpy8I GTNNAC 4 cut(s) 467, 1166, 1481, 1813
Hpy99I CGWCG 5 cut(s) 109, 272, 275, 308, 500
HpyAV CCTTC 5 cut(s) 202, 472, 827, 1789, 2186
HpyCH4III ACNGT 8 cut(s) 225, 1111, 1149, 1212, 1502, 1558, 1747, 2113
HpyCH4IV ACGT 6 cut(s) 104, 216, 338, 719, 1177, 1815
HpyF3I CTNAG 1 cut(s) 1329
HpySE526I ACGT 6 cut(s) 104, 216, 338, 719, 1177, 1815
Hsp92I GRCGYC 4 cut(s) 126, 381, 443, 495
Hsp92II CATG 9 cut(s) 238, 349, 601, 659, 669, 1073, 1549, 1721, 2245
HspAI GCGC 6 cut(s) 79, 116, 126, 358, 443, 503
KasI GGCGCC 2 cut(s) 125, 442
KroI GCCGGC 1 cut(s) 317
KroNI GCCGGC 1 cut(s) 319
Ksp22I TGATCA 1 cut(s) 1350
Kzo9I GATC 6 cut(s) 644, 694, 700, 709, 1350, 1675
LguI GCTCTTC 1 cut(s) 1536
LmnI GCTCC 1 cut(s) 725
Lsp1109I GCAGC 9 cut(s) 367, 663, 718, 740, 1029, 1510, 1513, 1593, 1750
MaeII ACGT 6 cut(s) 104, 216, 338, 719, 1177, 1815
MalI GATC 6 cut(s) 646, 696, 702, 711, 1352, 1677
MboI GATC 6 cut(s) 644, 694, 700, 709, 1350, 1675
MfeI CAATTG 1 cut(s) 855
MflI RGATCY 1 cut(s) 709
MhlI GDGCHC 5 cut(s) 597, 817, 871, 1909, 1967
MluCI AATT 8 cut(s) 397, 738, 855, 1027, 1092, 1572, 1583, 1919
Mly113I GGCGCC 2 cut(s) 126, 443
MlyI GAGTC 1 cut(s) 270
MmeI TCCRAC 3 cut(s) 253, 366, 1971
MroNI GCCGGC 1 cut(s) 317
MroXI GAANNNNTTC 2 cut(s) 176, 514
MseI TTAA 9 cut(s) 71, 324, 537, 1037, 1278, 1613, 1977, 2168, 2300
MslI CAYNNNNRTG 6 cut(s) 233, 609, 670, 802, 1254, 1550
MspA1I CMGCKG 6 cut(s) 85, 731, 845, 891, 1412, 1741
MspCI CTTAAG 1 cut(s) 2167
MspI CCGG 6 cut(s) 50, 318, 462, 470, 507, 980
MspR9I CCNGG 2 cut(s) 50, 507
MunI CAATTG 1 cut(s) 855
Mva1269I GAATGC 2 cut(s) 284, 1473
MvnI CGCG 3 cut(s) 79, 419, 699
NaeI GCCGGC 1 cut(s) 319
NarI GGCGCC 2 cut(s) 126, 443
NciI CCSGG 2 cut(s) 50, 507
NcoI CCATGG 1 cut(s) 597
NdeII GATC 6 cut(s) 644, 694, 700, 709, 1350, 1675
NgoMIV GCCGGC 1 cut(s) 317
NlaIII CATG 9 cut(s) 238, 349, 601, 659, 669, 1073, 1549, 1721, 2245
NlaIV GGNNCC 8 cut(s) 48, 127, 444, 460, 595, 1543, 1844, 2174
NmuCI GTSAC 6 cut(s) 334, 427, 1247, 1363, 1435, 2127
NruI TCGCGA 1 cut(s) 699
PaeR7I CTCGAG 1 cut(s) 167
PagI TCATGA 1 cut(s) 234
PaqCI CACCTGC 2 cut(s) 100, 2277
PciSI GCTCTTC 1 cut(s) 1536
PcsI WCGNNNNNNNCGW 2 cut(s) 150, 165
PctI GAATGC 2 cut(s) 284, 1473
PdiI GCCGGC 1 cut(s) 319
PdmI GAANNNNTTC 2 cut(s) 176, 514
PfeI GAWTC 6 cut(s) 53, 163, 1325, 1489, 1970, 2304
Ple19I CGATCG 1 cut(s) 703
PleI GAGTC 1 cut(s) 270
PluTI GGCGCC 2 cut(s) 129, 446
PmaCI CACGTG 2 cut(s) 217, 1178
PmlI CACGTG 2 cut(s) 217, 1178
PpsI GAGTC 1 cut(s) 270
Ppu21I YACGTR 2 cut(s) 217, 1178
PpuMI RGGWCCY 1 cut(s) 46
PshBI ATTAAT 1 cut(s) 1613
PsiI TTATAA 1 cut(s) 1089
Psp124BI GAGCTC 1 cut(s) 871
Psp5II RGGWCCY 1 cut(s) 46
PspCI CACGTG 2 cut(s) 217, 1178
PspEI GGTNACC 1 cut(s) 427
PspFI CCCAGC 1 cut(s) 1516
PspN4I GGNNCC 8 cut(s) 48, 127, 444, 460, 595, 1543, 1844, 2174
PspOMI GGGCCC 1 cut(s) 593
PspPI GGNCC 6 cut(s) 46, 211, 459, 593, 594, 1766
PspPPI RGGWCCY 1 cut(s) 46
PstI CTGCAG 1 cut(s) 562
PstNI CAGNNNCTG 1 cut(s) 734
PsuI RGATCY 1 cut(s) 709
PvuI CGATCG 1 cut(s) 703
PvuII CAGCTG 4 cut(s) 731, 845, 1412, 1741
RruI TCGCGA 1 cut(s) 699
RsaI GTAC 9 cut(s) 89, 227, 485, 774, 913, 1108, 1425, 1812, 1825
RsaNI GTAC 9 cut(s) 88, 226, 484, 773, 912, 1107, 1424, 1811, 1824
RseI CAYNNNNRTG 6 cut(s) 233, 609, 670, 802, 1254, 1550
SacI GAGCTC 1 cut(s) 871
SalI GTCGAC 1 cut(s) 1164
SapI GCTCTTC 1 cut(s) 1536
SaqAI TTAA 9 cut(s) 71, 324, 537, 1037, 1278, 1613, 1977, 2168, 2300
Sau3AI GATC 6 cut(s) 644, 694, 700, 709, 1350, 1675
Sau96I GGNCC 6 cut(s) 46, 211, 459, 593, 594, 1766
SchI GAGTC 1 cut(s) 270
ScrFI CCNGG 2 cut(s) 50, 507
SduI GDGCHC 5 cut(s) 597, 817, 871, 1909, 1967
SfcI CTRYAG 3 cut(s) 558, 886, 1827
SfoI GGCGCC 2 cut(s) 127, 444
Sfr274I CTCGAG 1 cut(s) 167
SinI GGWCC 2 cut(s) 46, 459
SlaI CTCGAG 1 cut(s) 167
SmiMI CAYNNNNRTG 6 cut(s) 233, 609, 670, 802, 1254, 1550
SmlI CTYRAG 3 cut(s) 167, 1378, 2167
SmoI CTYRAG 3 cut(s) 167, 1378, 2167
Sse9I AATT 8 cut(s) 397, 738, 855, 1027, 1092, 1572, 1583, 1919
SspDI GGCGCC 2 cut(s) 125, 442
SspI AATATT 1 cut(s) 1340
SstI GAGCTC 1 cut(s) 871
StyD4I CCNGG 2 cut(s) 48, 505
StyI CCWWGG 2 cut(s) 597, 991
TaaI ACNGT 8 cut(s) 225, 1111, 1149, 1212, 1502, 1558, 1747, 2113
TaiI ACGT 6 cut(s) 107, 219, 341, 722, 1180, 1818
TasI AATT 8 cut(s) 397, 738, 855, 1027, 1092, 1572, 1583, 1919
TatI WGTACW 3 cut(s) 225, 1106, 1810
TauI GCSGC 5 cut(s) 41, 452, 455, 1008, 1738
TfiI GAWTC 6 cut(s) 53, 163, 1325, 1489, 1970, 2304
Tru1I TTAA 9 cut(s) 71, 324, 537, 1037, 1278, 1613, 1977, 2168, 2300
Tru9I TTAA 9 cut(s) 71, 324, 537, 1037, 1278, 1613, 1977, 2168, 2300
TscAI CASTG 4 cut(s) 556, 567, 1998, 2136
TseFI GTSAC 6 cut(s) 334, 427, 1247, 1363, 1435, 2127
TseI GCWGC 9 cut(s) 355, 676, 728, 731, 1017, 1523, 1526, 1606, 1738
Tsp45I GTSAC 6 cut(s) 334, 427, 1247, 1363, 1435, 2127
TspDTI ATGAA 5 cut(s) 223, 251, 1700, 2062, 2230
TspGWI ACGGA 2 cut(s) 1811, 1892
TspRI CASTG 4 cut(s) 556, 567, 1998, 2136
Vha464I CTTAAG 1 cut(s) 2167
VpaK11BI GGWCC 2 cut(s) 46, 459
VspI ATTAAT 1 cut(s) 1613
XapI RAATTY 2 cut(s) 397, 1092
XcmI CCANNNNNNNNNTGG 1 cut(s) 109
XhoI CTCGAG 1 cut(s) 167
XmiI GTMKAC 2 cut(s) 466, 1165
XmnI GAANNNNTTC 2 cut(s) 176, 514
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.