Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0011015

Physical Location & Seq

Genomic Coordinates Forward (+)

1886131 .. 1889981

Gene Structure 3851 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G002290.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2334 bp

ATGTCGAGTAACGGAATCGACAACGGCGAGGACCCGGAGTCGATCACGGCGCCGTTACTTTCCGGCCGCCGATTCGCCCTCAACAACCACACCTCCCAGGTGGCCCTCGTCGGCGCCAATGTGTCCCCAATCGAAAGCCTCGACTACGAGATTTTGGAGAACGAGTTCTTCAAGCAGGACTGGCGCAGCCGCGAGACCGTGCACGTCTTCCAGTACATCTTCATGAAGTGGTTCACCTGCTTCCTCGTCGGAATTATCGTCGGAATGATCGGTTTCTGCAACAACCTCGCCGTCGAGAACATCGCCGGCGTCAAGTTCGTCATCACCTCCAACATGATGCTGGAGCGGAGGTTTTGGATGGCGTTTGTTGTCTTCTTCTCTTATAACTTGGCGCTCACTGTGTTTGCGTCTACTATCACTTCTTTAGTGGCGCCGGCGGCTGCCGGCTCCGGCATACCGGAGGTGAAGGCTTACCTTAATGGAGTGGACGCTCCGGGGATTTTTTCAATTCGGACTTTGATCGTAAAGATAGTTGGCAGTATTACTGCTGTGTCATCGTCTCTTTTAATTGGGAAGGCAGGGCCGATGGTGCATACTGGCGCATGTGTTGCATCATTGTTGGGACAGGGTGGATCAAAGAAGCATGGTTTGACGTGGAAGTGGCTACGATACTTCAAGAATGATCGAGACCGTAGGGATCTTGTGACTTGTGGATCAGCTGCTGGAATTGCTGCCTCTTTCCGTTCACCTGTTGGTGGTCTGCTCTTTGCCCTTGAAGAGATGGCATCTTGGTGGCGAGGAGCCCTTTTGTGGAGAGCTTTTTTCACAACTGCTGTAATTGCGATTGTGCTCCGAGCTTGGATTGATTTTTGCTTGAGTGGAAAATGTGGGCTATTTGGTACCGGAGGCCTGATAATGTTTGACGTATATTCATCGAATATGTCTTATCACCTAACAGATGTGCCTCCTGTGCTACTCCTGGCATTTGTAGGTGGCATATTGGGGAGCTTCTACAATTATCTATTAACAAAAGTTCTCCGAATTTACAATCTCATCCACGAGAAAGGCATCTTTTATAAAGTTCTTCTTGCTTGCTCAATTTCCATTTTCACATCTTGTCTTCTCTTTGGATTGCCATGGCTTGCATCTTGCCGACCTTGCCCACCTGATGCATCAGAGGCCTGTCCTACAATTGGAAGGTCCGGTAACTACAAGAAGTTCCAATGTCCTCCTGGTCACTATAATGACCTTGCCAGCCTCATATTTAACACAAATGATGATGCTATCAGAAATCTTTTCAGCAAGGACACTGATACCGAGTTTCAATACTCCTCAGTTCTCATATTTTTAATCTCCTGCTTTTTCTTAAGTATCTTTAGCTATGGGGTTGTTGTTCCTGCTGGTTTGTTTGTACCTGTTATTGTGACTGGTGCATCATATGGCCGCTTTGTTGGAATGTTGGTGGGTTCATACACAGATCTCAATCATGGCCTTTATGCTGTGCTGGGGGCTGCTTCTTTACTTGGTGGAACCATGAGGATGACCGTGTCTTTGTGTGTAATTATTCTGGAGCTGACCAATAATCTGTTATTGCTACCATTGATAATGATAGTTCTTCTTGTTTCCAAGACCACAGCAGATGCTTTCAATGGCAACATATACAATCTTATTATGAAAGCAAAGGGTCTTCCATATTTAAAGGGTCATGCTGAACCATATATGAGGCAGCTGACAGTAAGTGACGTAGTGACGGGGCCTCTTCAACTCTTTAATGGCATTGAGAAAGTTGGTAATATAGTACATGTTCTCCGAACTACAGGGCATAATGGTTTCCCTGTAATTGATGAGTCTCCACCACCTGAACCTCCTGTTTTGTTTGGTTTGGTCCTCCGTGCCCATCTTATTGAATTGTTGAAGAAGAAATCTTTTGTGTCTACTCCAGTGCGGACGACTACTGATGCCTTCAGACAGTTTTCGTCGGAGGATTTTGCAAAGAGGGGTTCAGGCAATGGTGACAAGATAGACGACATAGAACTGACTGAGGAGGAGATGGAAATGTACATAGACTTGCATCCATTTACTAACGCTTCACCTTATACTGTTGTGGAGACAATGTCATTAGCAAAGGCTCTCATACTTTTCCGACAAGTTGGTTTGAGGCACCTTCTGGTGATACCAAAGATGTCCTGTAGATCTCCTATAGTGGGCATATTGACAAGGCACGACTTCATGCCAGAACATATATTCCGCTTGCACCCTACGCTGGTAAGTAGCAGGTGGAAAAGATTAAGACTCCGGCTGCCCGTTCGAGAAAAATCTTTTAGGCTATCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005216 GO:0005244 GO:0005247 GO:0005253 GO:0005254 GO:0005575 GO:0006810 GO:0006811 GO:0006820 GO:0006821 GO:0008150 GO:0008308 GO:0008509 GO:0015075 GO:0015103 GO:0015108 GO:0015267 GO:0015318 GO:0015698 GO:0016020 GO:0022803 GO:0022832 GO:0022836 GO:0022838 GO:0022839 GO:0022857 GO:0034220 GO:0051179 GO:0051234 GO:0055085 GO:0098656 GO:0098660 GO:0098661 GO:1902476

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CBS Voltage_CLC

Protein Analysis

777

Amino Acids

85.79

Weight (kDa)

8.86

Isoelectric Point (pI)

43.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Voltage_CLC	PF00654	134 - 546	1.6e-79	Voltage gated chloride channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0011015)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G33280
fragaria_vesca	FvH4_1g02460 FvH4_1g02460 FvH4_1g02460 FvH4_1g02460
malus_domestica	MD02G1021600.v1.1 MD15G1164600.v1.1
prunus_persica	Prupe.7G250100_v2.0.a1
pyrus_communis	pycom02g01900 pycom15g14760
rosa_chinensis	RchiOBHm_Chr2g0087431
rosa_laevigata	RLG00000015868
rosa_multiflora	Rmu_sc0006064.1_g000011
rosa_roxburghii	Rroxscaffold_2G00153540
rosa_rugosa	Rorug01G0475300
rosa_samantha	Rh2AG028700 Rh2BG029000 Rh2CG029000 Rh2DG028800
rosa_wichuraiana	Rw2G002290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	384, 1077
AarI	CACCTGC	2 cut(s)	245, 2265
Acc36I	ACCTGC	2 cut(s)	245, 2265
Acc65I	GGTACC	1 cut(s)	899
AccB1I	GGYRCC	5 cut(s)	49, 113, 430, 899, 2160
AccBSI	CCGCTC	1 cut(s)	346
AccI	GTMKAC	2 cut(s)	410, 1934
AccII	CGCG	1 cut(s)	192
AciI	CCGC	7 cut(s)	67, 190, 346, 437, 1444, 1945, 2248
AclWI	GGATC	3 cut(s)	640, 705, 721
AcoI	YGGCCR	2 cut(s)	64, 1441
AcsI	RAATTY	1 cut(s)	1041
AcuI	CTGAAG	1 cut(s)	1948
AcyI	GRCGYC	4 cut(s)	50, 114, 309, 431
AfaI	GTAC	5 cut(s)	215, 901, 1413, 1800, 2060
AfiI	CCNNNNNNNGG	5 cut(s)	755, 810, 1193, 2204, 2263
AflII	CTTAAG	1 cut(s)	1366
AflIII	ACRYGT	1 cut(s)	1801
AgsI	TTSAA	9 cut(s)	172, 507, 676, 776, 1325, 1648, 1763, 1907, 1915
AhdI	GACNNNNNGTC	1 cut(s)	37
AjiI	CACGTC	2 cut(s)	205, 654
AjnI	CCWGG	3 cut(s)	96, 978, 1231
AloI	GAACNNNNNNTCC	4 cut(s)	149, 181, 2229, 2261
AluBI	AGCT	7 cut(s)	719, 818, 857, 1008, 1380, 1573, 1729
AluI	AGCT	7 cut(s)	719, 818, 857, 1008, 1380, 1573, 1729
Alw21I	GWGCWC	2 cut(s)	204, 852
Alw26I	GTCTC	5 cut(s)	188, 564, 681, 1854, 2102
Alw44I	GTGCAC	1 cut(s)	200
AlwI	GGATC	3 cut(s)	640, 705, 721
AlwNI	CAGNNNCTG	1 cut(s)	722
AoxI	GGCC	8 cut(s)	64, 102, 581, 907, 1179, 1441, 1489, 1754
ApaLI	GTGCAC	1 cut(s)	200
ApeKI	GCWGC	7 cut(s)	186, 440, 719, 731, 1511, 1726, 2299
ApoI	RAATTY	1 cut(s)	1041
ArsI	GACNNNNNNTTYG	2 cut(s)	1534, 1566
Asp700I	GAANNNNTTC	2 cut(s)	164, 230
Asp718I	GGTACC	1 cut(s)	899
AspLEI	GCGC	6 cut(s)	52, 116, 186, 394, 433, 602
AspS9I	GGNCC	6 cut(s)	31, 103, 581, 1200, 1754, 1885
AsuC2I	CCSGG	2 cut(s)	35, 495
AsuHPI	GGTGA	8 cut(s)	226, 316, 475, 738, 941, 2024, 2082, 2182
AvaII	GGWCC	3 cut(s)	31, 1200, 1885
BaeGI	GKGCMC	2 cut(s)	204, 1897
BanI	GGYRCC	5 cut(s)	49, 113, 430, 899, 2160
BanII	GRGCYC	1 cut(s)	805
BauI	CACGAG	1 cut(s)	1058
BbsI	GAAGAC	4 cut(s)	199, 364, 1112, 1679
Bbv12I	GWGCWC	2 cut(s)	204, 852
BbvI	GCAGC	7 cut(s)	198, 427, 706, 718, 1498, 1738, 2286
BccI	CCATC	5 cut(s)	352, 580, 775, 1905, 2044
BceAI	ACGGC	4 cut(s)	37, 40, 63, 275
BcgI	CGANNNNNNTGC	2 cut(s)	268, 302
BciT130I	CCWGG	3 cut(s)	98, 980, 1233
BcnI	CCSGG	2 cut(s)	35, 495
BcoDI	GTCTC	5 cut(s)	188, 564, 681, 1854, 2102
BfmI	CTRYAG	3 cut(s)	1815, 2188, 2199
BfoI	RGCGCY	4 cut(s)	53, 117, 395, 434
BfrI	CTTAAG	1 cut(s)	1366
BfuAI	ACCTGC	2 cut(s)	245, 2265
BglII	AGATCT	2 cut(s)	1477, 2192
Bme1390I	CCNGG	5 cut(s)	35, 98, 495, 980, 1233
Bme18I	GGWCC	3 cut(s)	31, 1200, 1885
BmeRI	GACNNNNNGTC	1 cut(s)	37
BmgBI	CACGTC	2 cut(s)	205, 654
BmgT120I	GGNCC	6 cut(s)	31, 103, 581, 1200, 1754, 1885
BmrFI	CCNGG	5 cut(s)	35, 98, 495, 980, 1233
BpiI	GAAGAC	4 cut(s)	199, 364, 1112, 1679
BpmI	CTGGAG	3 cut(s)	362, 1589, 1923
BpuEI	CTTGAG	1 cut(s)	895
BpuMI	CCSGG	2 cut(s)	35, 495
BsaBI	GATNNNNATC	1 cut(s)	1482
BsaHI	GRCGYC	4 cut(s)	50, 114, 309, 431
BsaI	GGTCTC	2 cut(s)	188, 681
BsaJI	CCNNGG	3 cut(s)	96, 494, 1136
BsaWI	WCCGGW	3 cut(s)	457, 902, 1202
Bsc4I	CCNNNNNNNGG	5 cut(s)	755, 810, 1193, 2204, 2263
Bse118I	RCCGGY	3 cut(s)	305, 433, 443
Bse1I	ACTGG	5 cut(s)	185, 211, 601, 1432, 1940
Bse3DI	GCAATG	1 cut(s)	2014
Bse8I	GATNNNNATC	1 cut(s)	1482
BseBI	CCWGG	3 cut(s)	98, 980, 1233
BseDI	CCNNGG	3 cut(s)	96, 494, 1136
BseGI	GGATG	4 cut(s)	363, 1053, 1545, 2071
BseJI	GATNNNNATC	1 cut(s)	1482
BseLI	CCNNNNNNNGG	5 cut(s)	755, 810, 1193, 2204, 2263
BseMI	GCAATG	1 cut(s)	2014
BseMII	CTCAG	2 cut(s)	1347, 2031
BseNI	ACTGG	5 cut(s)	185, 211, 601, 1432, 1940
BseRI	GAGGAG	4 cut(s)	813, 1321, 2057, 2060
BseSI	GKGCMC	2 cut(s)	204, 1897
BseX3I	CGGCCG	1 cut(s)	64
BseXI	GCAGC	7 cut(s)	198, 427, 706, 718, 1498, 1738, 2286
BseYI	CCCAGC	1 cut(s)	1504
Bsh1236I	CGCG	1 cut(s)	192
Bsh1285I	CGRYCG	1 cut(s)	67
BshFI	GGCC	8 cut(s)	66, 104, 583, 909, 1181, 1443, 1491, 1756
BshNI	GGYRCC	5 cut(s)	49, 113, 430, 899, 2160
BsiEI	CGRYCG	1 cut(s)	67
BsiHKAI	GWGCWC	2 cut(s)	204, 852
BslFI	GGGAC	2 cut(s)	109, 636
BslI	CCNNNNNNNGG	5 cut(s)	755, 810, 1193, 2204, 2263
BsmAI	GTCTC	5 cut(s)	188, 564, 681, 1854, 2102
BsmBI	CGTCTC	1 cut(s)	564
BsmFI	GGGAC	2 cut(s)	109, 636
BsnI	GGCC	8 cut(s)	66, 104, 583, 909, 1181, 1443, 1491, 1756
Bso31I	GGTCTC	2 cut(s)	188, 681
Bsp1286I	GDGCHC	4 cut(s)	204, 805, 852, 1897
Bsp1407I	TGTACA	1 cut(s)	2058
Bsp143I	GATC	9 cut(s)	42, 267, 519, 632, 682, 697, 713, 1477, 2192
Bsp19I	CCATGG	1 cut(s)	1136
BspACI	CCGC	7 cut(s)	67, 190, 346, 437, 1444, 1945, 2248
BspANI	GGCC	8 cut(s)	66, 104, 583, 909, 1181, 1443, 1491, 1756
BspCNI	CTCAG	2 cut(s)	1346, 2032
BspFNI	CGCG	1 cut(s)	192
BspHI	TCATGA	1 cut(s)	222
BspMI	ACCTGC	2 cut(s)	245, 2265
BspPI	GGATC	3 cut(s)	640, 705, 721
BspT107I	GGYRCC	5 cut(s)	49, 113, 430, 899, 2160
BspTI	CTTAAG	1 cut(s)	1366
BspTNI	GGTCTC	2 cut(s)	188, 681
BsrBI	CCGCTC	1 cut(s)	346
BsrDI	GCAATG	1 cut(s)	2014
BsrFI	RCCGGY	3 cut(s)	305, 433, 443
BsrGI	TGTACA	1 cut(s)	2058
BsrI	ACTGG	5 cut(s)	185, 211, 601, 1432, 1940
BssAI	RCCGGY	3 cut(s)	305, 433, 443
BssECI	CCNNGG	3 cut(s)	96, 494, 1136
BssMI	GATC	9 cut(s)	42, 267, 519, 632, 682, 697, 713, 1477, 2192
BssNI	GRCGYC	4 cut(s)	50, 114, 309, 431
BssSI	CACGAG	1 cut(s)	1058
BssT1I	CCWWGG	1 cut(s)	1136
Bst2BI	CACGAG	1 cut(s)	1058
Bst2UI	CCWGG	3 cut(s)	98, 980, 1233
Bst4CI	ACNGT	7 cut(s)	199, 400, 692, 1546, 1735, 1971, 2101
Bst6I	CTCTTC	2 cut(s)	771, 1764
BstACI	GRCGYC	4 cut(s)	50, 114, 309, 431
BstAFI	CTTAAG	1 cut(s)	1366
BstAPI	GCANNNNNTGC	1 cut(s)	608
BstAUI	TGTACA	1 cut(s)	2058
BstC8I	GCNNGC	7 cut(s)	307, 435, 445, 1093, 1143, 1255, 2252
BstDEI	CTNAG	3 cut(s)	1333, 2040, 2331
BstDSI	CCRYGG	1 cut(s)	1136
BstF5I	GGATG	4 cut(s)	363, 1053, 1545, 2071
BstFNI	CGCG	1 cut(s)	192
BstH2I	RGCGCY	4 cut(s)	53, 117, 395, 434
BstHHI	GCGC	6 cut(s)	52, 116, 186, 394, 433, 602
BstKTI	GATC	9 cut(s)	45, 270, 522, 635, 685, 700, 716, 1480, 2195
BstMAI	GTCTC	5 cut(s)	188, 564, 681, 1854, 2102
BstMBI	GATC	9 cut(s)	42, 267, 519, 632, 682, 697, 713, 1477, 2192
BstMCI	CGRYCG	1 cut(s)	67
BstNI	CCWGG	3 cut(s)	98, 980, 1233
BstNSI	RCATGY	2 cut(s)	606, 1805
BstSCI	CCNGG	5 cut(s)	33, 96, 493, 978, 1231
BstSFI	CTRYAG	3 cut(s)	1815, 2188, 2199
BstSLI	GKGCMC	2 cut(s)	204, 1897
BstUI	CGCG	1 cut(s)	192
BstV1I	GCAGC	7 cut(s)	198, 427, 706, 718, 1498, 1738, 2286
BstV2I	GAAGAC	4 cut(s)	199, 364, 1112, 1679
BstX2I	RGATCY	3 cut(s)	697, 1477, 2192
BstYI	RGATCY	3 cut(s)	697, 1477, 2192
BstZI	CGGCCG	1 cut(s)	64
BsuRI	GGCC	8 cut(s)	66, 104, 583, 909, 1181, 1443, 1491, 1756
BtgI	CCRYGG	1 cut(s)	1136
BtgZI	GCGATG	1 cut(s)	286
BtrI	CACGTC	2 cut(s)	205, 654
BtsCI	GGATG	4 cut(s)	363, 1053, 1545, 2071
BtsIMutI	CAGTG	3 cut(s)	396, 1308, 1947
BveI	ACCTGC	2 cut(s)	245, 2265
Cac8I	GCNNGC	7 cut(s)	307, 435, 445, 1093, 1143, 1255, 2252
CaiI	CAGNNNCTG	1 cut(s)	722
CciI	TCATGA	1 cut(s)	222
CfoI	GCGC	6 cut(s)	52, 116, 186, 394, 433, 602
Cfr10I	RCCGGY	3 cut(s)	305, 433, 443
Cfr13I	GGNCC	6 cut(s)	31, 103, 581, 1200, 1754, 1885
CseI	GACGC	3 cut(s)	298, 396, 497
Csp6I	GTAC	5 cut(s)	214, 900, 1412, 1799, 2059
CviQI	GTAC	5 cut(s)	214, 900, 1412, 1799, 2059
DdeI	CTNAG	3 cut(s)	1333, 2040, 2331
DinI	GGCGCC	3 cut(s)	51, 115, 432
DpnI	GATC	9 cut(s)	44, 269, 521, 634, 684, 699, 715, 1479, 2194
DpnII	GATC	9 cut(s)	42, 267, 519, 632, 682, 697, 713, 1477, 2192
DraI	TTTAAA	1 cut(s)	1698
DriI	GACNNNNNGTC	1 cut(s)	37
EaeI	YGGCCR	2 cut(s)	64, 1441
EagI	CGGCCG	1 cut(s)	64
Eam1104I	CTCTTC	2 cut(s)	771, 1764
Eam1105I	GACNNNNNGTC	1 cut(s)	37
EarI	CTCTTC	2 cut(s)	771, 1764
EclXI	CGGCCG	1 cut(s)	64
Eco130I	CCWWGG	1 cut(s)	1136
Eco147I	AGGCCT	2 cut(s)	909, 1181
Eco24I	GRGCYC	1 cut(s)	805
Eco31I	GGTCTC	2 cut(s)	188, 681
Eco47I	GGWCC	3 cut(s)	31, 1200, 1885
Eco52I	CGGCCG	1 cut(s)	64
Eco57I	CTGAAG	1 cut(s)	1948
EcoO109I	RGGNCCY	2 cut(s)	31, 1754
EcoRII	CCWGG	3 cut(s)	96, 978, 1231
EcoT14I	CCWWGG	1 cut(s)	1136
EcoT22I	ATGCAT	1 cut(s)	1174
EcoT38I	GRGCYC	1 cut(s)	805
EgeI	GGCGCC	3 cut(s)	51, 115, 432
EheI	GGCGCC	3 cut(s)	51, 115, 432
ErhI	CCWWGG	1 cut(s)	1136
Esp3I	CGTCTC	1 cut(s)	564
FalI	AAGNNNNNCTT	2 cut(s)	1071, 1103
FaqI	GGGAC	2 cut(s)	109, 636
FauNDI	CATATG	1 cut(s)	1438
FblI	GTMKAC	2 cut(s)	410, 1934
FokI	GGATG	4 cut(s)	370, 1040, 1552, 2058
FriOI	GRGCYC	1 cut(s)	805
GlaI	GCGC	6 cut(s)	51, 115, 185, 393, 432, 601
GsaI	CCCAGC	1 cut(s)	1508
GsuI	CTGGAG	3 cut(s)	362, 1589, 1923
HaeII	RGCGCY	4 cut(s)	53, 117, 395, 434
HaeIII	GGCC	8 cut(s)	66, 104, 583, 909, 1181, 1443, 1491, 1756
HgaI	GACGC	3 cut(s)	298, 396, 497
HhaI	GCGC	6 cut(s)	52, 116, 186, 394, 433, 602
Hin1I	GRCGYC	4 cut(s)	50, 114, 309, 431
Hin6I	GCGC	6 cut(s)	50, 114, 184, 392, 431, 600
HinP1I	GCGC	6 cut(s)	50, 114, 184, 392, 431, 600
HinfI	GANTC	5 cut(s)	15, 38, 72, 1847, 2292
HphI	GGTGA	8 cut(s)	226, 316, 475, 738, 941, 2024, 2082, 2182
Hpy166II	GTNNAC	6 cut(s)	202, 234, 411, 487, 746, 1935
Hpy188III	TCNNGA	6 cut(s)	223, 295, 676, 686, 1568, 2309
Hpy8I	GTNNAC	6 cut(s)	202, 234, 411, 487, 746, 1935
Hpy99I	CGWCG	5 cut(s)	113, 251, 263, 296, 1981
HpyAV	CCTTC	5 cut(s)	460, 568, 1191, 1972, 2174
HpyCH4III	ACNGT	7 cut(s)	199, 400, 692, 1546, 1735, 1971, 2101
HpyCH4IV	ACGT	4 cut(s)	204, 653, 924, 1743
HpyF3I	CTNAG	3 cut(s)	1333, 2040, 2331
HpySE526I	ACGT	4 cut(s)	204, 653, 924, 1743
Hsp92I	GRCGYC	4 cut(s)	50, 114, 309, 431
HspAI	GCGC	6 cut(s)	50, 114, 184, 392, 431, 600
KasI	GGCGCC	3 cut(s)	49, 113, 430
KpnI	GGTACC	1 cut(s)	903
KroI	GCCGGC	3 cut(s)	305, 433, 443
KroNI	GCCGGC	3 cut(s)	307, 435, 445
Kzo9I	GATC	9 cut(s)	42, 267, 519, 632, 682, 697, 713, 1477, 2192
LmnI	GCTCC	7 cut(s)	343, 452, 496, 800, 855, 1005, 1570
Lsp1109I	GCAGC	7 cut(s)	198, 427, 706, 718, 1498, 1738, 2286
MaeII	ACGT	4 cut(s)	204, 653, 924, 1743
MaeIII	GTNAC	9 cut(s)	8, 54, 703, 1205, 1235, 1423, 1739, 1747, 2012
MalI	GATC	9 cut(s)	44, 269, 521, 634, 684, 699, 715, 1479, 2194
MbiI	CCGCTC	1 cut(s)	346
MboI	GATC	9 cut(s)	42, 267, 519, 632, 682, 697, 713, 1477, 2192
MfeI	CAATTG	1 cut(s)	1191
MflI	RGATCY	3 cut(s)	697, 1477, 2192
MhlI	GDGCHC	4 cut(s)	204, 805, 852, 1897
Mly113I	GGCGCC	3 cut(s)	50, 114, 431
MlyI	GAGTC	3 cut(s)	47, 1856, 2286
MmeI	TCCRAC	6 cut(s)	229, 241, 354, 1432, 1959, 2167
Mph1103I	ATGCAT	1 cut(s)	1174
MreI	CGCCGGCG	2 cut(s)	305, 433
MroNI	GCCGGC	3 cut(s)	305, 433, 443
MroXI	GAANNNNTTC	2 cut(s)	164, 230
MseI	TTAA	9 cut(s)	477, 566, 1025, 1266, 1349, 1367, 1697, 1770, 2288
MslI	CAYNNNNRTG	4 cut(s)	221, 790, 1242, 1538
MspA1I	CMGCKG	2 cut(s)	719, 1729
MspCI	CTTAAG	1 cut(s)	1366
MspR9I	CCNGG	5 cut(s)	35, 98, 495, 980, 1233
MunI	CAATTG	1 cut(s)	1191
MvaI	CCWGG	3 cut(s)	98, 980, 1233
MvnI	CGCG	1 cut(s)	192
NaeI	GCCGGC	3 cut(s)	307, 435, 445
NarI	GGCGCC	3 cut(s)	50, 114, 431
NciI	CCSGG	2 cut(s)	35, 495
NcoI	CCATGG	1 cut(s)	1136
NdeI	CATATG	1 cut(s)	1438
NdeII	GATC	9 cut(s)	42, 267, 519, 632, 682, 697, 713, 1477, 2192
NgoMIV	GCCGGC	3 cut(s)	305, 433, 443
NmuCI	GTSAC	6 cut(s)	703, 1235, 1423, 1739, 1747, 2012
NsiI	ATGCAT	1 cut(s)	1174
NspI	RCATGY	2 cut(s)	606, 1805
PagI	TCATGA	1 cut(s)	222
PaqCI	CACCTGC	2 cut(s)	245, 2265
PceI	AGGCCT	2 cut(s)	909, 1181
PciI	ACATGT	1 cut(s)	1801
PcsI	WCGNNNNNNNCGW	3 cut(s)	24, 138, 255
PdiI	GCCGGC	3 cut(s)	307, 435, 445
PdmI	GAANNNNTTC	2 cut(s)	164, 230
PfeI	GAWTC	2 cut(s)	15, 72
PflFI	GACNNNGTC	2 cut(s)	1546, 2113
PleI	GAGTC	3 cut(s)	46, 1855, 2286
PluTI	GGCGCC	3 cut(s)	53, 117, 434
PpsI	GAGTC	3 cut(s)	46, 1855, 2286
PpuMI	RGGWCCY	1 cut(s)	31
PscI	ACATGT	1 cut(s)	1801
PsiI	TTATAA	2 cut(s)	384, 1077
Psp5II	RGGWCCY	1 cut(s)	31
Psp6I	CCWGG	3 cut(s)	96, 978, 1231
PspFI	CCCAGC	1 cut(s)	1504
PspGI	CCWGG	3 cut(s)	96, 978, 1231
PspPI	GGNCC	6 cut(s)	31, 103, 581, 1200, 1754, 1885
PspPPI	RGGWCCY	1 cut(s)	31
PstNI	CAGNNNCTG	1 cut(s)	722
PsuI	RGATCY	3 cut(s)	697, 1477, 2192
PsyI	GACNNNGTC	2 cut(s)	1546, 2113
PvuII	CAGCTG	2 cut(s)	719, 1729
RsaI	GTAC	5 cut(s)	215, 901, 1413, 1800, 2060
RsaNI	GTAC	5 cut(s)	214, 900, 1412, 1799, 2059
RseI	CAYNNNNRTG	4 cut(s)	221, 790, 1242, 1538
SaqAI	TTAA	9 cut(s)	477, 566, 1025, 1266, 1349, 1367, 1697, 1770, 2288
Sau3AI	GATC	9 cut(s)	42, 267, 519, 632, 682, 697, 713, 1477, 2192
Sau96I	GGNCC	6 cut(s)	31, 103, 581, 1200, 1754, 1885
SchI	GAGTC	3 cut(s)	47, 1856, 2286
ScrFI	CCNGG	5 cut(s)	35, 98, 495, 980, 1233
SduI	GDGCHC	4 cut(s)	204, 805, 852, 1897
SfcI	CTRYAG	3 cut(s)	1815, 2188, 2199
SfoI	GGCGCC	3 cut(s)	51, 115, 432
SgrAI	CRCCGGYG	2 cut(s)	305, 433
SinI	GGWCC	3 cut(s)	31, 1200, 1885
SmiMI	CAYNNNNRTG	4 cut(s)	221, 790, 1242, 1538
SmlI	CTYRAG	2 cut(s)	874, 1366
SmoI	CTYRAG	2 cut(s)	874, 1366
SseBI	AGGCCT	2 cut(s)	909, 1181
SsiI	CCGC	7 cut(s)	67, 190, 346, 437, 1444, 1945, 2248
SspDI	GGCGCC	3 cut(s)	49, 113, 430
StuI	AGGCCT	2 cut(s)	909, 1181
StyD4I	CCNGG	5 cut(s)	33, 96, 493, 978, 1231
StyI	CCWWGG	1 cut(s)	1136
TaaI	ACNGT	7 cut(s)	199, 400, 692, 1546, 1735, 1971, 2101
TaiI	ACGT	4 cut(s)	207, 656, 927, 1746
TaqI	TCGA	9 cut(s)	5, 18, 41, 132, 141, 294, 685, 935, 2308
TatI	WGTACW	3 cut(s)	213, 1798, 2058
TauI	GCSGC	4 cut(s)	69, 192, 440, 1446
TfiI	GAWTC	2 cut(s)	15, 72
Tru1I	TTAA	9 cut(s)	477, 566, 1025, 1266, 1349, 1367, 1697, 1770, 2288
Tru9I	TTAA	9 cut(s)	477, 566, 1025, 1266, 1349, 1367, 1697, 1770, 2288
TscAI	CASTG	3 cut(s)	403, 1315, 1947
TseFI	GTSAC	6 cut(s)	703, 1235, 1423, 1739, 1747, 2012
TseI	GCWGC	7 cut(s)	186, 440, 719, 731, 1511, 1726, 2299
Tsp45I	GTSAC	6 cut(s)	703, 1235, 1423, 1739, 1747, 2012
TspDTI	ATGAA	6 cut(s)	211, 239, 921, 1458, 1688, 2218
TspGWI	ACGGA	3 cut(s)	27, 731, 1880
TspRI	CASTG	3 cut(s)	403, 1315, 1947
Tth111I	GACNNNGTC	2 cut(s)	1546, 2113
Vha464I	CTTAAG	1 cut(s)	1366
VneI	GTGCAC	1 cut(s)	200
VpaK11BI	GGWCC	3 cut(s)	31, 1200, 1885
XapI	RAATTY	1 cut(s)	1041
XceI	RCATGY	2 cut(s)	606, 1805
XcmI	CCANNNNNNNNNTGG	2 cut(s)	337, 1229
XmiI	GTMKAC	2 cut(s)	410, 1934
XmnI	GAANNNNTTC	2 cut(s)	164, 230
Zsp2I	ATGCAT	1 cut(s)	1174

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.