Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0011015

Physical Location & Seq

Genomic Coordinates Reverse (-)

90342311 .. 90346079

Gene Structure 3769 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00153540.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2073 bp

ATGTCGAGTAACGGAATCCACAACGGCGAGGGCGAGGACGACCCGGAGTCAATCACGGCGCCGTTACTTTCCGGCCGCCGATACGCCCTCAACAACCACACCTCTCAGCTGGCCCTCGTCGGCGCCAATGTGTCCCCAATCGAAAGCCTCGACTACGAGATTTTGGAGAACGAGTTCTTCAAGCAGGACTGGCGCAGCCGCGAGACCGTCCACGTCTTCCAGTACATCTTCATGAAGTGGTTCACCTGTTTCCTCGTCGGAATTATCGTCGGAATTATCGGTTTCTGCAATAACCTCGCCGTCGAGAACATCGCCGGCGTCAAGTTCGTCATCACCTCCAACATGATGCTGGAGCAGAGGTTTTGGATGGCGTTTGTTGTCTTCTTCTCTTATAACTTGGCGCTCACTCTGTTTGCGTCTACTATCACTTGTTTAGTGGCGCCGGCGGCTGCCGGCTCCGGCATACCGGAGGTGAAGGCTTACCTTAATGGAGTCGACGCTCCGGGGATTTTTTCAATTCGGACTTTGATCGTAAAGATAGTTGGCAGTATTACTGCTGTGTCATCGTCTCTTTTAATTGGGAAGGCAGGGCCGATGGTGCATACTGGTGCATGTGTTGCATCATTGTTGGGACAGGGTGGATCAAAGAAGTATGGTTTGACATGGAAGTGGCTACGATACTTCAAGAATGATCGAGACCGTAGGGATCTTGTGACTTGTGGATCAGCTGCTGGAATTGCTGCCTCTTTCCGTTCACCTGTTGGTGGTCTGCTCTTTGCTCTTGAAGAGATGGCATCTTGGAAAGGCATCTTTTATAAAGTTCTTCTTGCTTGCTCAATTTCCATTTTCACATCTTGTCTTCTCTTTGGATTGCCATGGCTTGCATCTTGCCGACCTTGCCCACCTGATGCATCAGAGGCCTGTCCTACAATTGGAAGGTCCGGTAACTACAAGAAGTTCCAATGTCCTCCTGGTCACTATAATGACCTTGCCAGCCTCATATTTAACACAAATGATGATGCTATCAGAAATCTTTTCAGCAAGGACACTGATACCGAGTTTCAGTACTCCTCAGTTCTCATATTTTTAATCTTATGTTTTTTCTTAAGTATCTTTAGCTATGGGGTTGTTGTTCCTGCTGGTTTGTTTGTACCTGTTATTGTGACTGGTGCATCATATGGCCGCTTTGTTGGAATGTTGGTGGGTGCATACACAGATCTCAATCATGGCCTTTATGCTGTGCTGGGGGCTGCTTCTTTACTTGGTGGAACCATGAGGATGACCGTGTCTTTGTGTGTAATTATTCTGGAGCTGACCAATAATCTGTTGTTGCTACCATTGATAATGATAGTTCTTCTTGTTTCCAAGACCACAGCAGATGCTTTCAATGGCAACATATATGATCTTATCATGAAAGCAAAGGGTCTTCCATATTTAAAGGGTCATGCTGAACCATATATGAGGCAGCTGACAGTAAGTGACGTAGTGACGGGGCCACTTCAACTCTTTAATGGCATTGAGAAAGTTGGTAATATAGTACATGTTCTCAGAACTACAGGGCATAATGGTTTCCCTGTAATTGATGAGTCTCCACCACCTGAACCTCCTGTTTTGTTTGGTTTGGTCCTCCGTGCCCATCTTATTGAATTGTTGAAGAAGAAATCTTTTGTGTCTACCCCAGTGCGGACGACTACTGACGCCTTCAGACAGTTTTCGTCAGAGGATTTTGCAAAGAGGGGTTCAGGCAATGGTGACAAGATAGAAGACATAGAACTAACTGAGGAGGAGATGGAAATGTACATAGACTTGCATCCATTTACTAACGCTTCACCTTATACTGTTGTGGAGACAATGTCATTAGCAAAGGCTCTCATACTTTTCCGACAAGTTGGTTTGAGGCACCTTCTAGTGATACCAAAGATGTCCTGTAGATCTCCTATAGTGGGCATATTGACAAGGCACGACTTCATGCCAGAACATATATTCCGCTTGCACCCTACGCTGGTAAGTAGCAGGTGGAAAAGATTAAGACTCCGGCTGCCCGTTCGAGAAAAATCTTTTAGGCTGTCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005216 GO:0005244 GO:0005247 GO:0005253 GO:0005254 GO:0005575 GO:0006810 GO:0006811 GO:0006820 GO:0006821 GO:0008150 GO:0008308 GO:0008509 GO:0015075 GO:0015103 GO:0015108 GO:0015267 GO:0015318 GO:0015698 GO:0016020 GO:0022803 GO:0022832 GO:0022836 GO:0022838 GO:0022839 GO:0022857 GO:0034220 GO:0051179 GO:0051234 GO:0055085 GO:0098656 GO:0098660 GO:0098661 GO:1902476

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CBS Voltage_CLC

Protein Analysis

690

Amino Acids

76.07

Weight (kDa)

8.52

Isoelectric Point (pI)

45.34

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Voltage_CLC	PF00654	136 - 290	1.6e-36	Voltage gated chloride channel
Voltage_CLC	PF00654	280 - 459	1.4e-26	Voltage gated chloride channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011015)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G33280
fragaria_vesca	FvH4_1g02460 FvH4_1g02460 FvH4_1g02460 FvH4_1g02460
malus_domestica	MD02G1021600.v1.1 MD15G1164600.v1.1
prunus_persica	Prupe.7G250100_v2.0.a1
pyrus_communis	pycom02g01900 pycom15g14760
rosa_chinensis	RchiOBHm_Chr2g0087431
rosa_laevigata	RLG00000015868
rosa_multiflora	Rmu_sc0006064.1_g000011
rosa_roxburghii	Rroxscaffold_2G00153540
rosa_rugosa	Rorug01G0475300
rosa_samantha	Rh2AG028700 Rh2BG029000 Rh2CG029000 Rh2DG028800
rosa_wichuraiana	Rw2G002290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	393, 816
AarI	CACCTGC	1 cut(s)	2004
Acc36I	ACCTGC	1 cut(s)	2004
AccB1I	GGYRCC	4 cut(s)	58, 122, 439, 1899
AccI	GTMKAC	3 cut(s)	419, 495, 1673
AccII	CGCG	1 cut(s)	201
AciI	CCGC	6 cut(s)	76, 199, 446, 1183, 1684, 1987
AclWI	GGATC	3 cut(s)	649, 714, 730
AcoI	YGGCCR	2 cut(s)	73, 1180
AcuI	CTGAAG	1 cut(s)	1687
AcyI	GRCGYC	5 cut(s)	59, 123, 318, 440, 1698
AfaI	GTAC	5 cut(s)	224, 1067, 1152, 1539, 1799
AfiI	CCNNNNNNNGG	5 cut(s)	764, 932, 1683, 1943, 2002
AflII	CTTAAG	1 cut(s)	1105
AflIII	ACRYGT	1 cut(s)	1540
AgsI	TTSAA	8 cut(s)	181, 516, 685, 785, 1387, 1502, 1646, 1654
AhdI	GACNNNNNGTC	1 cut(s)	46
AjiI	CACGTC	1 cut(s)	214
AjnI	CCWGG	1 cut(s)	970
AloI	GAACNNNNNNTCC	4 cut(s)	158, 190, 1968, 2000
AluBI	AGCT	5 cut(s)	109, 728, 1119, 1312, 1468
AluI	AGCT	5 cut(s)	109, 728, 1119, 1312, 1468
Alw26I	GTCTC	5 cut(s)	197, 573, 690, 1593, 1841
AlwI	GGATC	3 cut(s)	649, 714, 730
AlwNI	CAGNNNCTG	1 cut(s)	731
AoxI	GGCC	7 cut(s)	73, 111, 590, 918, 1180, 1228, 1493
ApeKI	GCWGC	7 cut(s)	195, 449, 728, 740, 1250, 1465, 2038
ArsI	GACNNNNNNTTYG	2 cut(s)	1273, 1305
Asp700I	GAANNNNTTC	2 cut(s)	173, 239
AspLEI	GCGC	5 cut(s)	61, 125, 195, 403, 442
AspS9I	GGNCC	5 cut(s)	112, 590, 939, 1493, 1624
AsuC2I	CCSGG	2 cut(s)	44, 504
AsuHPI	GGTGA	6 cut(s)	235, 325, 484, 747, 1763, 1821
AvaII	GGWCC	2 cut(s)	939, 1624
BaeGI	GKGCMC	1 cut(s)	1636
BanI	GGYRCC	4 cut(s)	58, 122, 439, 1899
BbsI	GAAGAC	5 cut(s)	208, 373, 851, 1418, 1770
BbvI	GCAGC	7 cut(s)	207, 436, 715, 727, 1237, 1477, 2025
BccI	CCATC	5 cut(s)	361, 589, 784, 1644, 1783
BceAI	ACGGC	4 cut(s)	40, 46, 72, 284
BcgI	CGANNNNNNTGC	2 cut(s)	277, 311
BciT130I	CCWGG	1 cut(s)	972
BcnI	CCSGG	2 cut(s)	44, 504
BcoDI	GTCTC	5 cut(s)	197, 573, 690, 1593, 1841
BfaI	CTAG	1 cut(s)	1907
BfmI	CTRYAG	3 cut(s)	1554, 1927, 1938
BfoI	RGCGCY	4 cut(s)	62, 126, 404, 443
BfrI	CTTAAG	1 cut(s)	1105
BfuAI	ACCTGC	1 cut(s)	2004
BglII	AGATCT	2 cut(s)	1216, 1931
BmcAI	AGTACT	1 cut(s)	1067
Bme1390I	CCNGG	3 cut(s)	44, 504, 972
Bme18I	GGWCC	2 cut(s)	939, 1624
BmeRI	GACNNNNNGTC	1 cut(s)	46
BmgBI	CACGTC	1 cut(s)	214
BmgT120I	GGNCC	5 cut(s)	112, 590, 939, 1493, 1624
BmiI	GGNNCC	7 cut(s)	60, 124, 441, 457, 1270, 1494, 1901
BmrFI	CCNGG	3 cut(s)	44, 504, 972
BmrI	ACTGGG	1 cut(s)	1673
BmuI	ACTGGG	1 cut(s)	1673
BpiI	GAAGAC	5 cut(s)	208, 373, 851, 1418, 1770
BpmI	CTGGAG	2 cut(s)	371, 1328
BpuMI	CCSGG	2 cut(s)	44, 504
BsaBI	GATNNNNATC	1 cut(s)	1221
BsaHI	GRCGYC	5 cut(s)	59, 123, 318, 440, 1698
BsaI	GGTCTC	2 cut(s)	197, 690
BsaJI	CCNNGG	2 cut(s)	503, 875
BsaWI	WCCGGW	2 cut(s)	466, 941
BsaXI	ACNNNNNCTCC	6 cut(s)	158, 188, 344, 374, 1838, 1868
Bsc4I	CCNNNNNNNGG	5 cut(s)	764, 932, 1683, 1943, 2002
Bse118I	RCCGGY	3 cut(s)	314, 442, 452
Bse1I	ACTGG	5 cut(s)	194, 220, 610, 1171, 1679
Bse3DI	GCAATG	1 cut(s)	1753
Bse8I	GATNNNNATC	1 cut(s)	1221
BseBI	CCWGG	1 cut(s)	972
BseDI	CCNNGG	2 cut(s)	503, 875
BseGI	GGATG	3 cut(s)	372, 1284, 1810
BseJI	GATNNNNATC	1 cut(s)	1221
BseLI	CCNNNNNNNGG	5 cut(s)	764, 932, 1683, 1943, 2002
BseMI	GCAATG	1 cut(s)	1753
BseMII	CTCAG	4 cut(s)	119, 1086, 1561, 1770
BseNI	ACTGG	5 cut(s)	194, 220, 610, 1171, 1679
BseRI	GAGGAG	3 cut(s)	1060, 1796, 1799
BseSI	GKGCMC	1 cut(s)	1636
BseX3I	CGGCCG	1 cut(s)	73
BseXI	GCAGC	7 cut(s)	207, 436, 715, 727, 1237, 1477, 2025
BseYI	CCCAGC	1 cut(s)	1243
Bsh1236I	CGCG	1 cut(s)	201
Bsh1285I	CGRYCG	1 cut(s)	76
BshFI	GGCC	7 cut(s)	75, 113, 592, 920, 1182, 1230, 1495
BshNI	GGYRCC	4 cut(s)	58, 122, 439, 1899
BsiEI	CGRYCG	1 cut(s)	76
BslFI	GGGAC	2 cut(s)	118, 645
BslI	CCNNNNNNNGG	5 cut(s)	764, 932, 1683, 1943, 2002
BsmAI	GTCTC	5 cut(s)	197, 573, 690, 1593, 1841
BsmBI	CGTCTC	1 cut(s)	573
BsmFI	GGGAC	2 cut(s)	118, 645
BsnI	GGCC	7 cut(s)	75, 113, 592, 920, 1182, 1230, 1495
Bso31I	GGTCTC	2 cut(s)	197, 690
Bsp1286I	GDGCHC	1 cut(s)	1636
Bsp1407I	TGTACA	1 cut(s)	1797
Bsp143I	GATC	8 cut(s)	528, 641, 691, 706, 722, 1216, 1402, 1931
Bsp19I	CCATGG	1 cut(s)	875
BspACI	CCGC	6 cut(s)	76, 199, 446, 1183, 1684, 1987
BspANI	GGCC	7 cut(s)	75, 113, 592, 920, 1182, 1230, 1495
BspCNI	CTCAG	4 cut(s)	118, 1085, 1560, 1771
BspFNI	CGCG	1 cut(s)	201
BspHI	TCATGA	2 cut(s)	231, 1410
BspLI	GGNNCC	7 cut(s)	60, 124, 441, 457, 1270, 1494, 1901
BspMI	ACCTGC	1 cut(s)	2004
BspPI	GGATC	3 cut(s)	649, 714, 730
BspT107I	GGYRCC	4 cut(s)	58, 122, 439, 1899
BspTI	CTTAAG	1 cut(s)	1105
BspTNI	GGTCTC	2 cut(s)	197, 690
BsrDI	GCAATG	1 cut(s)	1753
BsrFI	RCCGGY	3 cut(s)	314, 442, 452
BsrGI	TGTACA	1 cut(s)	1797
BsrI	ACTGG	5 cut(s)	194, 220, 610, 1171, 1679
BssAI	RCCGGY	3 cut(s)	314, 442, 452
BssECI	CCNNGG	2 cut(s)	503, 875
BssMI	GATC	8 cut(s)	528, 641, 691, 706, 722, 1216, 1402, 1931
BssNI	GRCGYC	5 cut(s)	59, 123, 318, 440, 1698
BssT1I	CCWWGG	1 cut(s)	875
Bst2UI	CCWGG	1 cut(s)	972
Bst4CI	ACNGT	6 cut(s)	208, 701, 1285, 1474, 1710, 1840
Bst6I	CTCTTC	1 cut(s)	780
BstACI	GRCGYC	5 cut(s)	59, 123, 318, 440, 1698
BstAFI	CTTAAG	1 cut(s)	1105
BstAPI	GCANNNNNTGC	1 cut(s)	617
BstAUI	TGTACA	1 cut(s)	1797
BstC8I	GCNNGC	8 cut(s)	111, 316, 444, 454, 832, 882, 994, 1991
BstDEI	CTNAG	4 cut(s)	105, 1072, 1547, 1779
BstDSI	CCRYGG	1 cut(s)	875
BstF5I	GGATG	3 cut(s)	372, 1284, 1810
BstFNI	CGCG	1 cut(s)	201
BstH2I	RGCGCY	4 cut(s)	62, 126, 404, 443
BstHHI	GCGC	5 cut(s)	61, 125, 195, 403, 442
BstKTI	GATC	8 cut(s)	531, 644, 694, 709, 725, 1219, 1405, 1934
BstMAI	GTCTC	5 cut(s)	197, 573, 690, 1593, 1841
BstMBI	GATC	8 cut(s)	528, 641, 691, 706, 722, 1216, 1402, 1931
BstMCI	CGRYCG	1 cut(s)	76
BstMWI	GCNNNNNNNGC	8 cut(s)	190, 446, 598, 617, 737, 897, 917, 1999
BstNI	CCWGG	1 cut(s)	972
BstNSI	RCATGY	2 cut(s)	615, 1544
BstSCI	CCNGG	3 cut(s)	42, 502, 970
BstSFI	CTRYAG	3 cut(s)	1554, 1927, 1938
BstSLI	GKGCMC	1 cut(s)	1636
BstUI	CGCG	1 cut(s)	201
BstV1I	GCAGC	7 cut(s)	207, 436, 715, 727, 1237, 1477, 2025
BstV2I	GAAGAC	5 cut(s)	208, 373, 851, 1418, 1770
BstX2I	RGATCY	3 cut(s)	706, 1216, 1931
BstYI	RGATCY	3 cut(s)	706, 1216, 1931
BstZI	CGGCCG	1 cut(s)	73
BsuRI	GGCC	7 cut(s)	75, 113, 592, 920, 1182, 1230, 1495
BtgI	CCRYGG	1 cut(s)	875
BtgZI	GCGATG	1 cut(s)	295
BtrI	CACGTC	1 cut(s)	214
BtsCI	GGATG	3 cut(s)	372, 1284, 1810
BtsIMutI	CAGTG	2 cut(s)	1047, 1686
BveI	ACCTGC	1 cut(s)	2004
Cac8I	GCNNGC	8 cut(s)	111, 316, 444, 454, 832, 882, 994, 1991
CaiI	CAGNNNCTG	1 cut(s)	731
CciI	TCATGA	2 cut(s)	231, 1410
CfoI	GCGC	5 cut(s)	61, 125, 195, 403, 442
Cfr10I	RCCGGY	3 cut(s)	314, 442, 452
Cfr13I	GGNCC	5 cut(s)	112, 590, 939, 1493, 1624
CseI	GACGC	4 cut(s)	307, 405, 506, 1706
Csp6I	GTAC	5 cut(s)	223, 1066, 1151, 1538, 1798
CviQI	GTAC	5 cut(s)	223, 1066, 1151, 1538, 1798
DdeI	CTNAG	4 cut(s)	105, 1072, 1547, 1779
DinI	GGCGCC	3 cut(s)	60, 124, 441
DpnI	GATC	8 cut(s)	530, 643, 693, 708, 724, 1218, 1404, 1933
DpnII	GATC	8 cut(s)	528, 641, 691, 706, 722, 1216, 1402, 1931
DraI	TTTAAA	1 cut(s)	1437
DriI	GACNNNNNGTC	1 cut(s)	46
EaeI	YGGCCR	2 cut(s)	73, 1180
EagI	CGGCCG	1 cut(s)	73
Eam1104I	CTCTTC	1 cut(s)	780
Eam1105I	GACNNNNNGTC	1 cut(s)	46
EarI	CTCTTC	1 cut(s)	780
EclXI	CGGCCG	1 cut(s)	73
Eco130I	CCWWGG	1 cut(s)	875
Eco147I	AGGCCT	1 cut(s)	920
Eco31I	GGTCTC	2 cut(s)	197, 690
Eco47I	GGWCC	2 cut(s)	939, 1624
Eco52I	CGGCCG	1 cut(s)	73
Eco57I	CTGAAG	1 cut(s)	1687
EcoRII	CCWGG	1 cut(s)	970
EcoT14I	CCWWGG	1 cut(s)	875
EcoT22I	ATGCAT	1 cut(s)	913
EgeI	GGCGCC	3 cut(s)	60, 124, 441
EheI	GGCGCC	3 cut(s)	60, 124, 441
ErhI	CCWWGG	1 cut(s)	875
Esp3I	CGTCTC	1 cut(s)	573
FalI	AAGNNNNNCTT	2 cut(s)	810, 842
FaqI	GGGAC	2 cut(s)	118, 645
FauNDI	CATATG	1 cut(s)	1177
FblI	GTMKAC	3 cut(s)	419, 495, 1673
FokI	GGATG	3 cut(s)	379, 1291, 1797
FspBI	CTAG	1 cut(s)	1907
GlaI	GCGC	5 cut(s)	60, 124, 194, 402, 441
GsaI	CCCAGC	1 cut(s)	1247
GsuI	CTGGAG	2 cut(s)	371, 1328
HaeII	RGCGCY	4 cut(s)	62, 126, 404, 443
HaeIII	GGCC	7 cut(s)	75, 113, 592, 920, 1182, 1230, 1495
HgaI	GACGC	4 cut(s)	307, 405, 506, 1706
HhaI	GCGC	5 cut(s)	61, 125, 195, 403, 442
Hin1I	GRCGYC	5 cut(s)	59, 123, 318, 440, 1698
Hin6I	GCGC	5 cut(s)	59, 123, 193, 401, 440
HinP1I	GCGC	5 cut(s)	59, 123, 193, 401, 440
HincII	GTYRAC	1 cut(s)	496
HindII	GTYRAC	1 cut(s)	496
HinfI	GANTC	5 cut(s)	15, 47, 492, 1586, 2031
HphI	GGTGA	6 cut(s)	235, 325, 484, 747, 1763, 1821
Hpy166II	GTNNAC	6 cut(s)	211, 243, 420, 496, 755, 1674
Hpy188I	TCNGA	9 cut(s)	260, 272, 522, 916, 1028, 1550, 1706, 1720, 1883
Hpy188III	TCNNGA	8 cut(s)	232, 304, 685, 695, 782, 1307, 1411, 2048
Hpy8I	GTNNAC	6 cut(s)	211, 243, 420, 496, 755, 1674
Hpy99I	CGWCG	5 cut(s)	122, 260, 272, 305, 500
HpyAV	CCTTC	5 cut(s)	469, 577, 930, 1711, 1913
HpyCH4III	ACNGT	6 cut(s)	208, 701, 1285, 1474, 1710, 1840
HpyCH4IV	ACGT	2 cut(s)	213, 1482
HpyF10VI	GCNNNNNNNGC	8 cut(s)	190, 446, 598, 617, 737, 897, 917, 1999
HpyF3I	CTNAG	4 cut(s)	105, 1072, 1547, 1779
HpySE526I	ACGT	2 cut(s)	213, 1482
Hsp92I	GRCGYC	5 cut(s)	59, 123, 318, 440, 1698
HspAI	GCGC	5 cut(s)	59, 123, 193, 401, 440
KasI	GGCGCC	3 cut(s)	58, 122, 439
KroI	GCCGGC	3 cut(s)	314, 442, 452
KroNI	GCCGGC	3 cut(s)	316, 444, 454
Kzo9I	GATC	8 cut(s)	528, 641, 691, 706, 722, 1216, 1402, 1931
LmnI	GCTCC	4 cut(s)	352, 461, 505, 1309
Lsp1109I	GCAGC	7 cut(s)	207, 436, 715, 727, 1237, 1477, 2025
MaeI	CTAG	1 cut(s)	1907
MaeII	ACGT	2 cut(s)	213, 1482
MaeIII	GTNAC	9 cut(s)	8, 63, 712, 944, 974, 1162, 1478, 1486, 1751
MalI	GATC	8 cut(s)	530, 643, 693, 708, 724, 1218, 1404, 1933
MboI	GATC	8 cut(s)	528, 641, 691, 706, 722, 1216, 1402, 1931
MfeI	CAATTG	1 cut(s)	930
MflI	RGATCY	3 cut(s)	706, 1216, 1931
MhlI	GDGCHC	1 cut(s)	1636
Mly113I	GGCGCC	3 cut(s)	59, 123, 440
MlyI	GAGTC	4 cut(s)	56, 501, 1595, 2025
MmeI	TCCRAC	5 cut(s)	238, 250, 363, 1171, 1906
Mph1103I	ATGCAT	1 cut(s)	913
MreI	CGCCGGCG	2 cut(s)	314, 442
MroNI	GCCGGC	3 cut(s)	314, 442, 452
MroXI	GAANNNNTTC	2 cut(s)	173, 239
MseI	TTAA	8 cut(s)	486, 575, 1005, 1088, 1106, 1436, 1509, 2027
MslI	CAYNNNNRTG	5 cut(s)	230, 606, 667, 981, 1277
MspA1I	CMGCKG	3 cut(s)	109, 728, 1468
MspCI	CTTAAG	1 cut(s)	1105
MspR9I	CCNGG	3 cut(s)	44, 504, 972
MunI	CAATTG	1 cut(s)	930
MvaI	CCWGG	1 cut(s)	972
MvnI	CGCG	1 cut(s)	201
MwoI	GCNNNNNNNGC	8 cut(s)	190, 446, 598, 617, 737, 897, 917, 1999
NaeI	GCCGGC	3 cut(s)	316, 444, 454
NarI	GGCGCC	3 cut(s)	59, 123, 440
NciI	CCSGG	2 cut(s)	44, 504
NcoI	CCATGG	1 cut(s)	875
NdeI	CATATG	1 cut(s)	1177
NdeII	GATC	8 cut(s)	528, 641, 691, 706, 722, 1216, 1402, 1931
NgoMIV	GCCGGC	3 cut(s)	314, 442, 452
NlaIV	GGNNCC	7 cut(s)	60, 124, 441, 457, 1270, 1494, 1901
NmuCI	GTSAC	6 cut(s)	712, 974, 1162, 1478, 1486, 1751
NsiI	ATGCAT	1 cut(s)	913
NspI	RCATGY	2 cut(s)	615, 1544
PagI	TCATGA	2 cut(s)	231, 1410
PaqCI	CACCTGC	1 cut(s)	2004
PceI	AGGCCT	1 cut(s)	920
PciI	ACATGT	1 cut(s)	1540
PcsI	WCGNNNNNNNCGW	3 cut(s)	30, 147, 264
PdiI	GCCGGC	3 cut(s)	316, 444, 454
PdmI	GAANNNNTTC	2 cut(s)	173, 239
PfeI	GAWTC	1 cut(s)	15
PflFI	GACNNNGTC	2 cut(s)	1285, 1852
PleI	GAGTC	4 cut(s)	55, 500, 1594, 2025
PluTI	GGCGCC	3 cut(s)	62, 126, 443
PpsI	GAGTC	4 cut(s)	55, 500, 1594, 2025
PscI	ACATGT	1 cut(s)	1540
PsiI	TTATAA	2 cut(s)	393, 816
Psp6I	CCWGG	1 cut(s)	970
PspFI	CCCAGC	1 cut(s)	1243
PspGI	CCWGG	1 cut(s)	970
PspN4I	GGNNCC	7 cut(s)	60, 124, 441, 457, 1270, 1494, 1901
PspPI	GGNCC	5 cut(s)	112, 590, 939, 1493, 1624
PstNI	CAGNNNCTG	1 cut(s)	731
PsuI	RGATCY	3 cut(s)	706, 1216, 1931
PsyI	GACNNNGTC	2 cut(s)	1285, 1852
PvuII	CAGCTG	3 cut(s)	109, 728, 1468
RsaI	GTAC	5 cut(s)	224, 1067, 1152, 1539, 1799
RsaNI	GTAC	5 cut(s)	223, 1066, 1151, 1538, 1798
RseI	CAYNNNNRTG	5 cut(s)	230, 606, 667, 981, 1277
SalI	GTCGAC	1 cut(s)	494
SaqAI	TTAA	8 cut(s)	486, 575, 1005, 1088, 1106, 1436, 1509, 2027
Sau3AI	GATC	8 cut(s)	528, 641, 691, 706, 722, 1216, 1402, 1931
Sau96I	GGNCC	5 cut(s)	112, 590, 939, 1493, 1624
ScaI	AGTACT	1 cut(s)	1067
SchI	GAGTC	4 cut(s)	56, 501, 1595, 2025
ScrFI	CCNGG	3 cut(s)	44, 504, 972
SduI	GDGCHC	1 cut(s)	1636
SfcI	CTRYAG	3 cut(s)	1554, 1927, 1938
SfoI	GGCGCC	3 cut(s)	60, 124, 441
SgrAI	CRCCGGYG	2 cut(s)	314, 442
SinI	GGWCC	2 cut(s)	939, 1624
SmiMI	CAYNNNNRTG	5 cut(s)	230, 606, 667, 981, 1277
SmlI	CTYRAG	1 cut(s)	1105
SmoI	CTYRAG	1 cut(s)	1105
SseBI	AGGCCT	1 cut(s)	920
SsiI	CCGC	6 cut(s)	76, 199, 446, 1183, 1684, 1987
SspDI	GGCGCC	3 cut(s)	58, 122, 439
SspMI	CTAG	1 cut(s)	1907
StuI	AGGCCT	1 cut(s)	920
StyD4I	CCNGG	3 cut(s)	42, 502, 970
StyI	CCWWGG	1 cut(s)	875
TaaI	ACNGT	6 cut(s)	208, 701, 1285, 1474, 1710, 1840
TaiI	ACGT	2 cut(s)	216, 1485
TaqI	TCGA	7 cut(s)	5, 141, 150, 303, 495, 694, 2047
TatI	WGTACW	4 cut(s)	222, 1065, 1537, 1797
TauI	GCSGC	4 cut(s)	78, 201, 449, 1185
TfiI	GAWTC	1 cut(s)	15
Tru1I	TTAA	8 cut(s)	486, 575, 1005, 1088, 1106, 1436, 1509, 2027
Tru9I	TTAA	8 cut(s)	486, 575, 1005, 1088, 1106, 1436, 1509, 2027
TscAI	CASTG	2 cut(s)	1054, 1686
TseFI	GTSAC	6 cut(s)	712, 974, 1162, 1478, 1486, 1751
TseI	GCWGC	7 cut(s)	195, 449, 728, 740, 1250, 1465, 2038
Tsp45I	GTSAC	6 cut(s)	712, 974, 1162, 1478, 1486, 1751
TspDTI	ATGAA	4 cut(s)	220, 248, 1427, 1957
TspGWI	ACGGA	3 cut(s)	27, 740, 1619
TspRI	CASTG	2 cut(s)	1054, 1686
Tth111I	GACNNNGTC	2 cut(s)	1285, 1852
Vha464I	CTTAAG	1 cut(s)	1105
VpaK11BI	GGWCC	2 cut(s)	939, 1624
XceI	RCATGY	2 cut(s)	615, 1544
XcmI	CCANNNNNNNNNTGG	2 cut(s)	346, 968
XmiI	GTMKAC	3 cut(s)	419, 495, 1673
XmnI	GAANNNNTTC	2 cut(s)	173, 239
XspI	CTAG	1 cut(s)	1907
ZrmI	AGTACT	1 cut(s)	1067
Zsp2I	ATGCAT	1 cut(s)	913

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.