pycom02g05230

3-hydroxyisobutyrate dehydrogenase-like 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0016018
Physical Location & Seq
Forward (+)
3455487 .. 3456533
1047 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g05230.1

Sequence Viewer

Length: 1047 bp
ATGACAAAACTCAAAATAGTCAAGCTATTTCCACTTCCACTCCTCCGTCCACTCTCTCGCTCCACCAGGACCGCCACTCTCTCCCTCCTCCTCCGCCGTCGCCACATGGCCACCGTCGCGTTATCCGATTCCCCCATAAGCCCGTCAGACACCCGGGTCGGGTGGATCGGAACCGGCGTTATGGGCCGGTCCATGTGCACCCACCTCCTCAAGGCTGGCTACGCCCTCACCGTCTTCAACCGCACCCTCTCCAAGGCCCAGCCTCTCGTCGACCTCGGGGCCCACCTGGCCGACTCCCCAAGCGCCGTCGCCTCCAAATCCGACGTCGTCTTTTCCATCGTCGGCTACCCCTCCGACGTCCGCTCCGTCCTCCTCGACTCCACCACCGGCGCACTCTCTGGACTGCGCCCTGGAGGCATCCTCGTCGACATGACCACCTCCGAACCCTCCCTCGCCGTCGAGATCTCCTCCGCCGCTGAGTCCAAGTCCTGCTCAGCTGTCGATGCCCCCGTCACCGGCGGCGACGTTGGCGCCAAGAACGGAACTCTCGCGATCCTCGCCGGCGGCGACGAAAACACCGTCCGCAAGCTAACCCCTCTGTTCTCCCTCATGGGCAAAGTGAATTATATGGGCGGCGCCGGAAAGGGACAGTTCGCGAAGCTGGCGAACCAGACGATCATCGCTTCCACGATGGTGGGTCTGGTGGAGGGTATGATCTTCGCCCACAAGTCCGGCCTCGACGTGGGTCTTTTCCTAGATGCGATCAAAGTGGGCGCCGCAGGGTCGAAGTCGCTGGATTTGTACGGCGGCCGGATTCTGAAGAGGGATTTGGAGCCGGGATTCTATGTGAACCACTTCGTGAAAGATTTGGGGATTTGCTTGAAGGAGTGCCAGAAGATGGGACTTGCTCTTCCCGGATTAGCCCTGGCTCAGCAGCTTTATCTGTCGCTGAAGGCTCACGGCGAGGGCAATTTGGGAACTCAGGCGCTTATTGTGGCGGTGGAGCGGCTGAATAATGTGTCACTTGAATATGCTCCTGCAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAD_binding_11 NAD_binding_2

Protein Analysis

349

Amino Acids

36.41

Weight (kDa)

9.44

Isoelectric Point (pI)

35.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Shikimate_DH PF01488 49 - 114 2.9e-06 Shikimate / quinate 5-dehydrogenase
NAD_binding_2 PF03446 52 - 210 3.3e-46 NAD binding domain of 6-phosphogluconate dehydrogenase
NAD_binding_11 PF14833 214 - 331 5.8e-33 NAD-binding of NADP-dependent 3-hydroxyisobutyrate dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016018)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G29120
fragaria_vesca FvH4_1g06170
malus_domestica MD02G1065900.v1.1 MD02G1066000.v1.1
prunus_persica Prupe.7G219600_v2.0.a1
pyrus_communis pycom02g05230
rosa_chinensis RchiOBHm_Chr2g0091951
rosa_laevigata RLG00000016265
rosa_multiflora Rmu_sc0009216.1_g000005 Rmu_sc0017125.1_g000007
rosa_roxburghii Rroxscaffold_2G00149590
rosa_rugosa Rorug02G0021800
rosa_samantha Rh2AG067400 Rh2DG066500
rosa_wichuraiana Rw2G005360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 155
AatII GACGTC 2 cut(s) 327, 360
AccB1I GGYRCC 3 cut(s) 530, 635, 773
AccB7I CCANNNNNTGG 1 cut(s) 898
AccBSI CCGCTC 2 cut(s) 363, 1006
AccI GTMKAC 2 cut(s) 270, 426
AccII CGCG 3 cut(s) 119, 551, 656
AclWI GGATC 2 cut(s) 173, 547
AcoI YGGCCR 3 cut(s) 108, 288, 808
AcuI CTGAAG 2 cut(s) 839, 971
AcyI GRCGYC 5 cut(s) 324, 357, 531, 636, 774
AdeI CACNNNGTG 1 cut(s) 859
AfaI GTAC 1 cut(s) 803
AfiI CCNNNNNNNGG 6 cut(s) 159, 211, 253, 515, 742, 898
AgsI TTSAA 3 cut(s) 238, 883, 1028
AjiI CACGTC 1 cut(s) 742
AjnI CCWGG 4 cut(s) 65, 285, 409, 924
AjuI GAANNNNNNNTTGG 2 cut(s) 812, 844
AleI CACNNNNGTG 1 cut(s) 692
AluBI AGCT 5 cut(s) 25, 497, 589, 661, 937
AluI AGCT 5 cut(s) 25, 497, 589, 661, 937
Alw21I GWGCWC 1 cut(s) 200
Alw44I GTGCAC 1 cut(s) 196
AlwI GGATC 2 cut(s) 173, 547
Ama87I CYCGRG 2 cut(s) 153, 275
AoxI GGCC 7 cut(s) 108, 184, 255, 279, 288, 733, 808
ApaI GGGCCC 1 cut(s) 283
ApaLI GTGCAC 1 cut(s) 196
ApeKI GCWGC 1 cut(s) 934
ArsI GACNNNNNNTTYG 4 cut(s) 309, 341, 564, 596
Asp700I GAANNNNTTC 1 cut(s) 854
AspLEI GCGC 7 cut(s) 305, 392, 408, 533, 638, 776, 988
AspS9I GGNCC 6 cut(s) 69, 184, 189, 256, 279, 280
AsuC2I CCSGG 4 cut(s) 154, 155, 837, 915
AsuHPI GGTGA 2 cut(s) 220, 505
AvaI CYCGRG 2 cut(s) 153, 275
AvaII GGWCC 2 cut(s) 69, 189
BaeGI GKGCMC 2 cut(s) 200, 283
BalI TGGCCA 1 cut(s) 110
BanI GGYRCC 3 cut(s) 530, 635, 773
BanII GRGCYC 1 cut(s) 283
BbsI GAAGAC 1 cut(s) 226
Bbv12I GWGCWC 1 cut(s) 200
BbvI GCAGC 1 cut(s) 946
BccI CCATC 3 cut(s) 344, 685, 892
BceAI ACGGC 5 cut(s) 81, 290, 440, 820, 976
BcgI CGANNNNNNTGC 2 cut(s) 406, 440
BciT130I CCWGG 4 cut(s) 67, 287, 411, 926
BcnI CCSGG 4 cut(s) 154, 155, 837, 915
BfaI CTAG 1 cut(s) 755
BfoI RGCGCY 5 cut(s) 306, 534, 639, 777, 989
BglI GCCNNNNNGGC 2 cut(s) 287, 414
BglII AGATCT 1 cut(s) 462
BisI GCNGC 8 cut(s) 474, 520, 565, 634, 777, 808, 935, 1007
BlpI GCTNAGC 2 cut(s) 493, 930
BlsI GCNGC 8 cut(s) 475, 521, 566, 635, 778, 809, 936, 1008
Bme1390I CCNGG 8 cut(s) 67, 154, 155, 287, 411, 837, 915, 926
Bme18I GGWCC 2 cut(s) 69, 189
BmeT110I CYCGRG 2 cut(s) 153, 275
BmgBI CACGTC 1 cut(s) 742
BmgT120I GGNCC 6 cut(s) 69, 184, 189, 256, 279, 280
BmiI GGNNCC 7 cut(s) 172, 280, 281, 532, 637, 775, 834
BmrFI CCNGG 8 cut(s) 67, 154, 155, 287, 411, 837, 915, 926
BmsI GCATC 3 cut(s) 426, 493, 748
BoxI GACNNNNGTC 1 cut(s) 744
BpiI GAAGAC 1 cut(s) 226
BplI GAGNNNNNCTC 4 cut(s) 405, 437, 452, 484
BpmI CTGGAG 1 cut(s) 432
Bpu1102I GCTNAGC 2 cut(s) 493, 930
BpuEI CTTGAG 1 cut(s) 194
BpuMI CCSGG 4 cut(s) 154, 155, 837, 915
BsaHI GRCGYC 5 cut(s) 324, 357, 531, 636, 774
BsaJI CCNNGG 5 cut(s) 153, 252, 274, 409, 924
BsaXI ACNNNNNCTCC 4 cut(s) 24, 54, 347, 377
Bsc4I CCNNNNNNNGG 6 cut(s) 159, 211, 253, 515, 742, 898
Bse118I RCCGGY 5 cut(s) 173, 186, 386, 515, 560
BseBI CCWGG 4 cut(s) 67, 287, 411, 926
BseDI CCNNGG 5 cut(s) 153, 252, 274, 409, 924
BseGI GGATG 1 cut(s) 417
BseLI CCNNNNNNNGG 6 cut(s) 159, 211, 253, 515, 742, 898
BseMII CTCAG 4 cut(s) 468, 507, 944, 995
BseRI GAGGAG 6 cut(s) 32, 77, 80, 197, 362, 457
BseSI GKGCMC 2 cut(s) 200, 283
BseX3I CGGCCG 1 cut(s) 808
BseXI GCAGC 1 cut(s) 946
BseYI CCCAGC 1 cut(s) 258
Bsh1236I CGCG 3 cut(s) 119, 551, 656
Bsh1285I CGRYCG 1 cut(s) 811
BshFI GGCC 7 cut(s) 110, 186, 257, 281, 290, 735, 810
BshNI GGYRCC 3 cut(s) 530, 635, 773
BsiEI CGRYCG 1 cut(s) 811
BsiHKAI GWGCWC 1 cut(s) 200
BsiHKCI CYCGRG 2 cut(s) 153, 275
BslFI GGGAC 2 cut(s) 660, 915
BslI CCNNNNNNNGG 6 cut(s) 159, 211, 253, 515, 742, 898
BsmFI GGGAC 2 cut(s) 660, 915
BsnI GGCC 7 cut(s) 110, 186, 257, 281, 290, 735, 810
BsoBI CYCGRG 2 cut(s) 153, 275
Bsp120I GGGCCC 1 cut(s) 279
Bsp1286I GDGCHC 2 cut(s) 200, 283
Bsp143I GATC 6 cut(s) 165, 462, 552, 675, 714, 762
Bsp1720I GCTNAGC 2 cut(s) 493, 930
Bsp68I TCGCGA 2 cut(s) 551, 656
BspANI GGCC 7 cut(s) 110, 186, 257, 281, 290, 735, 810
BspCNI CTCAG 4 cut(s) 469, 506, 943, 994
BspFNI CGCG 3 cut(s) 119, 551, 656
BspLI GGNNCC 7 cut(s) 172, 280, 281, 532, 637, 775, 834
BspPI GGATC 2 cut(s) 173, 547
BspQI GCTCTTC 1 cut(s) 915
BspT107I GGYRCC 3 cut(s) 530, 635, 773
BsrBI CCGCTC 2 cut(s) 363, 1006
BsrFI RCCGGY 5 cut(s) 173, 186, 386, 515, 560
BssAI RCCGGY 5 cut(s) 173, 186, 386, 515, 560
BssECI CCNNGG 5 cut(s) 153, 252, 274, 409, 924
BssMI GATC 6 cut(s) 165, 462, 552, 675, 714, 762
BssNI GRCGYC 5 cut(s) 324, 357, 531, 636, 774
BssT1I CCWWGG 1 cut(s) 252
Bst2UI CCWGG 4 cut(s) 67, 287, 411, 926
Bst4CI ACNGT 4 cut(s) 115, 232, 580, 651
Bst6I CTCTTC 2 cut(s) 815, 915
BstACI GRCGYC 5 cut(s) 324, 357, 531, 636, 774
BstC8I GCNNGC 4 cut(s) 217, 562, 587, 663
BstDEI CTNAG 4 cut(s) 477, 493, 930, 981
BstENI CCTNNNNNAGG 2 cut(s) 209, 251
BstF5I GGATG 1 cut(s) 417
BstFNI CGCG 3 cut(s) 119, 551, 656
BstH2I RGCGCY 5 cut(s) 306, 534, 639, 777, 989
BstHHI GCGC 7 cut(s) 305, 392, 408, 533, 638, 776, 988
BstKTI GATC 6 cut(s) 168, 465, 555, 678, 717, 765
BstMBI GATC 6 cut(s) 165, 462, 552, 675, 714, 762
BstMCI CGRYCG 1 cut(s) 811
BstMWI GCNNNNNNNGC 9 cut(s) 116, 183, 221, 287, 414, 503, 528, 557, 662
BstNI CCWGG 4 cut(s) 67, 287, 411, 926
BstPAI GACNNNNGTC 1 cut(s) 744
BstSCI CCNGG 8 cut(s) 65, 152, 153, 285, 409, 835, 913, 924
BstSLI GKGCMC 2 cut(s) 200, 283
BstUI CGCG 3 cut(s) 119, 551, 656
BstV1I GCAGC 1 cut(s) 946
BstV2I GAAGAC 1 cut(s) 226
BstX2I RGATCY 1 cut(s) 462
BstXI CCANNNNNNTGG 1 cut(s) 694
BstYI RGATCY 1 cut(s) 462
BstZI CGGCCG 1 cut(s) 808
BsuRI GGCC 7 cut(s) 110, 186, 257, 281, 290, 735, 810
BtgZI GCGATG 1 cut(s) 664
BtrI CACGTC 1 cut(s) 742
BtsCI GGATG 1 cut(s) 417
BtuMI TCGCGA 2 cut(s) 551, 656
Cac8I GCNNGC 4 cut(s) 217, 562, 587, 663
CfoI GCGC 7 cut(s) 305, 392, 408, 533, 638, 776, 988
Cfr10I RCCGGY 5 cut(s) 173, 186, 386, 515, 560
Cfr13I GGNCC 6 cut(s) 69, 184, 189, 256, 279, 280
Cfr9I CCCGGG 1 cut(s) 153
Csp6I GTAC 1 cut(s) 802
CviAII CATG 4 cut(s) 106, 193, 430, 610
CviQI GTAC 1 cut(s) 802
DdeI CTNAG 4 cut(s) 477, 493, 930, 981
DinI GGCGCC 3 cut(s) 532, 637, 775
DpnI GATC 6 cut(s) 167, 464, 554, 677, 716, 764
DpnII GATC 6 cut(s) 165, 462, 552, 675, 714, 762
DraIII CACNNNGTG 1 cut(s) 859
DrdI GACNNNNNNGTC 1 cut(s) 155
DseDI GACNNNNNNGTC 1 cut(s) 155
EaeI YGGCCR 3 cut(s) 108, 288, 808
EagI CGGCCG 1 cut(s) 808
Eam1104I CTCTTC 2 cut(s) 815, 915
EarI CTCTTC 2 cut(s) 815, 915
EciI GGCGGA 2 cut(s) 83, 460
EclXI CGGCCG 1 cut(s) 808
Eco130I CCWWGG 1 cut(s) 252
Eco24I GRGCYC 1 cut(s) 283
Eco47I GGWCC 2 cut(s) 69, 189
Eco52I CGGCCG 1 cut(s) 808
Eco57I CTGAAG 2 cut(s) 839, 971
Eco88I CYCGRG 2 cut(s) 153, 275
EcoNI CCTNNNNNAGG 2 cut(s) 209, 251
EcoO109I RGGNCCY 1 cut(s) 279
EcoRII CCWGG 4 cut(s) 65, 285, 409, 924
EcoT14I CCWWGG 1 cut(s) 252
EcoT38I GRGCYC 1 cut(s) 283
EgeI GGCGCC 3 cut(s) 532, 637, 775
EheI GGCGCC 3 cut(s) 532, 637, 775
ErhI CCWWGG 1 cut(s) 252
FaeI CATG 4 cut(s) 109, 196, 433, 613
FaqI GGGAC 2 cut(s) 660, 915
FatI CATG 4 cut(s) 105, 192, 429, 609
FblI GTMKAC 2 cut(s) 270, 426
Fnu4HI GCNGC 8 cut(s) 474, 520, 565, 634, 777, 808, 935, 1007
FokI GGATG 1 cut(s) 404
FriOI GRGCYC 1 cut(s) 283
Fsp4HI GCNGC 8 cut(s) 474, 520, 565, 634, 777, 808, 935, 1007
FspBI CTAG 1 cut(s) 755
GlaI GCGC 7 cut(s) 304, 391, 407, 532, 637, 775, 987
GluI GCNGC 8 cut(s) 474, 520, 565, 634, 777, 808, 935, 1007
GsaI CCCAGC 1 cut(s) 262
GsuI CTGGAG 1 cut(s) 432
HaeII RGCGCY 5 cut(s) 306, 534, 639, 777, 989
HaeIII GGCC 7 cut(s) 110, 186, 257, 281, 290, 735, 810
HhaI GCGC 7 cut(s) 305, 392, 408, 533, 638, 776, 988
Hin1I GRCGYC 5 cut(s) 324, 357, 531, 636, 774
Hin1II CATG 4 cut(s) 109, 196, 433, 613
Hin6I GCGC 7 cut(s) 303, 390, 406, 531, 636, 774, 986
HinP1I GCGC 7 cut(s) 303, 390, 406, 531, 636, 774, 986
HincII GTYRAC 2 cut(s) 271, 427
HindII GTYRAC 2 cut(s) 271, 427
HinfI GANTC 6 cut(s) 128, 293, 377, 479, 814, 840
HphI GGTGA 2 cut(s) 220, 505
Hpy166II GTNNAC 5 cut(s) 50, 198, 271, 427, 850
Hpy188I TCNGA 7 cut(s) 127, 148, 170, 322, 355, 442, 819
Hpy188III TCNNGA 5 cut(s) 399, 460, 550, 655, 859
Hpy8I GTNNAC 5 cut(s) 50, 198, 271, 427, 850
HpyAV CCTTC 2 cut(s) 877, 946
HpyCH4III ACNGT 4 cut(s) 115, 232, 580, 651
HpyCH4IV ACGT 4 cut(s) 324, 357, 525, 741
HpyCH4V TGCA 2 cut(s) 198, 1040
HpyF10VI GCNNNNNNNGC 9 cut(s) 116, 183, 221, 287, 414, 503, 528, 557, 662
HpyF3I CTNAG 4 cut(s) 477, 493, 930, 981
HpySE526I ACGT 4 cut(s) 324, 357, 525, 741
Hsp92I GRCGYC 5 cut(s) 324, 357, 531, 636, 774
Hsp92II CATG 4 cut(s) 109, 196, 433, 613
HspAI GCGC 7 cut(s) 303, 390, 406, 531, 636, 774, 986
KasI GGCGCC 3 cut(s) 530, 635, 773
KroI GCCGGC 1 cut(s) 560
KroNI GCCGGC 1 cut(s) 562
Kzo9I GATC 6 cut(s) 165, 462, 552, 675, 714, 762
LguI GCTCTTC 1 cut(s) 915
LmnI GCTCC 5 cut(s) 65, 368, 832, 1003, 1039
Lsp1109I GCAGC 1 cut(s) 946
LweI GCATC 3 cut(s) 426, 493, 748
MaeI CTAG 1 cut(s) 755
MaeII ACGT 4 cut(s) 324, 357, 525, 741
MaeIII GTNAC 2 cut(s) 511, 1020
MalI GATC 6 cut(s) 167, 464, 554, 677, 716, 764
MbiI CCGCTC 2 cut(s) 363, 1006
MboI GATC 6 cut(s) 165, 462, 552, 675, 714, 762
MboII GAAGA 5 cut(s) 226, 709, 832, 902, 907
MflI RGATCY 1 cut(s) 462
MhlI GDGCHC 2 cut(s) 200, 283
MlsI TGGCCA 1 cut(s) 110
MluCI AATT 2 cut(s) 622, 970
MluNI TGGCCA 1 cut(s) 110
Mly113I GGCGCC 3 cut(s) 531, 636, 774
MlyI GAGTC 3 cut(s) 287, 371, 488
MmeI TCCRAC 2 cut(s) 345, 378
Mox20I TGGCCA 1 cut(s) 110
MreI CGCCGGCG 1 cut(s) 560
MroNI GCCGGC 1 cut(s) 560
MroXI GAANNNNTTC 1 cut(s) 854
MscI TGGCCA 1 cut(s) 110
MslI CAYNNNNRTG 1 cut(s) 692
Msp20I TGGCCA 1 cut(s) 110
MspA1I CMGCKG 2 cut(s) 476, 497
MspR9I CCNGG 8 cut(s) 67, 154, 155, 287, 411, 837, 915, 926
MvaI CCWGG 4 cut(s) 67, 287, 411, 926
MvnI CGCG 3 cut(s) 119, 551, 656
MwoI GCNNNNNNNGC 9 cut(s) 116, 183, 221, 287, 414, 503, 528, 557, 662
NaeI GCCGGC 1 cut(s) 562
NarI GGCGCC 3 cut(s) 531, 636, 774
NciI CCSGG 4 cut(s) 154, 155, 837, 915
NdeII GATC 6 cut(s) 165, 462, 552, 675, 714, 762
NgoMIV GCCGGC 1 cut(s) 560
NlaIII CATG 4 cut(s) 109, 196, 433, 613
NlaIV GGNNCC 7 cut(s) 172, 280, 281, 532, 637, 775, 834
NmuCI GTSAC 2 cut(s) 511, 1020
NruI TCGCGA 2 cut(s) 551, 656
OliI CACNNNNGTG 1 cut(s) 692
PciSI GCTCTTC 1 cut(s) 915
PcsI WCGNNNNNNNCGW 7 cut(s) 123, 174, 228, 363, 507, 564, 576
PdiI GCCGGC 1 cut(s) 562
PdmI GAANNNNTTC 1 cut(s) 854
PfeI GAWTC 3 cut(s) 128, 814, 840
PflFI GACNNNGTC 1 cut(s) 326
PflMI CCANNNNNTGG 1 cut(s) 898
PfoI TCCNGGA 1 cut(s) 913
PkrI GCNGC 8 cut(s) 475, 521, 566, 635, 778, 809, 936, 1008
PleI GAGTC 3 cut(s) 287, 371, 487
PluTI GGCGCC 3 cut(s) 534, 639, 777
PpsI GAGTC 3 cut(s) 287, 371, 487
PshAI GACNNNNGTC 1 cut(s) 744
Psp6I CCWGG 4 cut(s) 65, 285, 409, 924
PspFI CCCAGC 1 cut(s) 258
PspGI CCWGG 4 cut(s) 65, 285, 409, 924
PspN4I GGNNCC 7 cut(s) 172, 280, 281, 532, 637, 775, 834
PspOMI GGGCCC 1 cut(s) 279
PspPI GGNCC 6 cut(s) 69, 184, 189, 256, 279, 280
PsuI RGATCY 1 cut(s) 462
PsyI GACNNNGTC 1 cut(s) 326
PvuII CAGCTG 1 cut(s) 497
RruI TCGCGA 2 cut(s) 551, 656
RsaI GTAC 1 cut(s) 803
RsaNI GTAC 1 cut(s) 802
RseI CAYNNNNRTG 1 cut(s) 692
SalI GTCGAC 2 cut(s) 269, 425
SapI GCTCTTC 1 cut(s) 915
SatI GCNGC 8 cut(s) 474, 520, 565, 634, 777, 808, 935, 1007
Sau3AI GATC 6 cut(s) 165, 462, 552, 675, 714, 762
Sau96I GGNCC 6 cut(s) 69, 184, 189, 256, 279, 280
SchI GAGTC 3 cut(s) 287, 371, 488
ScrFI CCNGG 8 cut(s) 67, 154, 155, 287, 411, 837, 915, 926
SduI GDGCHC 2 cut(s) 200, 283
SfaNI GCATC 3 cut(s) 426, 493, 748
SfiI GGCCNNNNNGGCC 1 cut(s) 287
SfoI GGCGCC 3 cut(s) 532, 637, 775
SgrAI CRCCGGYG 3 cut(s) 386, 515, 560
SinI GGWCC 2 cut(s) 69, 189
SmaI CCCGGG 1 cut(s) 155
SmiMI CAYNNNNRTG 1 cut(s) 692
SmlI CTYRAG 1 cut(s) 209
SmoI CTYRAG 1 cut(s) 209
Sse9I AATT 2 cut(s) 622, 970
SspDI GGCGCC 3 cut(s) 530, 635, 773
SspMI CTAG 1 cut(s) 755
StyD4I CCNGG 8 cut(s) 65, 152, 153, 285, 409, 835, 913, 924
StyI CCWWGG 1 cut(s) 252
TaaI ACNGT 4 cut(s) 115, 232, 580, 651
TaiI ACGT 4 cut(s) 327, 360, 528, 744
TaqI TCGA 7 cut(s) 270, 375, 426, 459, 501, 738, 785
TasI AATT 2 cut(s) 622, 970
TauI GCSGC 7 cut(s) 476, 522, 567, 636, 779, 810, 1009
TfiI GAWTC 3 cut(s) 128, 814, 840
TseFI GTSAC 2 cut(s) 511, 1020
TseI GCWGC 1 cut(s) 934
Tsp45I GTSAC 2 cut(s) 511, 1020
TspGWI ACGGA 3 cut(s) 35, 355, 555
TspMI CCCGGG 1 cut(s) 153
Tth111I GACNNNGTC 1 cut(s) 326
Van91I CCANNNNNTGG 1 cut(s) 898
VneI GTGCAC 1 cut(s) 196
VpaK11BI GGWCC 2 cut(s) 69, 189
XagI CCTNNNNNAGG 2 cut(s) 209, 251
XmaI CCCGGG 1 cut(s) 153
XmiI GTMKAC 2 cut(s) 270, 426
XmnI GAANNNNTTC 1 cut(s) 854
XspI CTAG 1 cut(s) 755
ZraI GACGTC 2 cut(s) 325, 358
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.