Rh2DG066500

3-hydroxyisobutyrate dehydrogenase-like 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0016018
Physical Location & Seq
Forward (+)
4985363 .. 4987022
1660 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG066500.1

Sequence Viewer

Length: 1047 bp
ATGATCCTCAAAATAGTCAAACACATTTCACCTCCACTCCTTCCCCAACTCTCCCATCACTTCCACTCCCACTTCACCGCTCTCCTCCGCCGCACCATGGCCGCCGCCGCCTCCTCCACCGAGCCCACTATAAGCCCATCAACCACCCGCGTGGGCTGGATCGGCACCGGAGTCATGGGCCGGTCCATGTGCGGCCACCTCCTCAAAGCCGGCTACACTCTCACCATCTTCAACCGCACCCTATCCAAGGCCCAGCCGCTTCTCGATTTGGGCGCCAAACTCGCCGATTCCCCACGCGCCGTCGCCTCCCAATCCGACGTCGTCTTCTCCATCGTCGGCTACCCCTCCGACGTCCGCTCCGTCCTCCTCGACCCCACCTCCGGCGCCCTCTCCGGCCTCCGCCCCGGCGGCGTCCTTGTCGACATGACCACCTCCGAGCCCTCCCTCGCCGTCGAGATCTCCCAATCCGCCGCCGCCAAATCCTGCTCCTCGGTCGACGCGCCCGTCACCGGCGGAGACGTTGGCGCGAAAAACGGGACCTTAGCGATTCTGGCGGGCGGCGACGAAGCCGTCGTGGGGAAGCTGATTCCCCTTTTTGCCCTTATGGGTAAGGTTAATTACATGGGTGCCCCCGGGAAGGGCCAGTTTGCAAAGCTAGCGAACCAGACCATCATATGTTCAACAATGTTGGGCCTGGTAGAAGGCATGGTTTTTGCCCACAAGGCTGGGCTTGATGTGGGCCTGTTCTTGAAGGCAATCTCGGTCGGGGCGGCCGGGTCCAGGTCGTTGGATTTGCACGGAGCTAAGATTCTGAAGAGGGATTTCGAGCCGGGATTCTATGTGAATCACTTTGTGAAGGACTTGGGAATTTGTTTGAAGGAGTGTCAGAACATGGGGCTGGCTCTGCCGGGGTTGGCTCTGGCTCAGCAGCTTTACTTGTCACTCAAGGCTCATGGTGAGGGCAATTTGGGCACCCAAGCGCTCATTGTGGCTCTGGAGCGCCTCAACAATGTGTCACTTGAACAACAGTCTGGGTTGACAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAD_binding_11 NAD_binding_2

Protein Analysis

348

Amino Acids

36.25

Weight (kDa)

9.16

Isoelectric Point (pI)

35.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Shikimate_DH PF01488 47 - 112 9.1e-06 Shikimate / quinate 5-dehydrogenase
NAD_binding_2 PF03446 50 - 208 6.6e-45 NAD binding domain of 6-phosphogluconate dehydrogenase
NAD_binding_11 PF14833 212 - 330 2.7e-33 NAD-binding of NADP-dependent 3-hydroxyisobutyrate dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016018)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G29120
fragaria_vesca FvH4_1g06170
malus_domestica MD02G1065900.v1.1 MD02G1066000.v1.1
prunus_persica Prupe.7G219600_v2.0.a1
pyrus_communis pycom02g05230
rosa_chinensis RchiOBHm_Chr2g0091951
rosa_laevigata RLG00000016265
rosa_multiflora Rmu_sc0009216.1_g000005 Rmu_sc0017125.1_g000007
rosa_roxburghii Rroxscaffold_2G00149590
rosa_rugosa Rorug02G0021800
rosa_samantha Rh2AG067400 Rh2DG066500
rosa_wichuraiana Rw2G005360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 503, 569
AatII GACGTC 2 cut(s) 321, 354
AccB1I GGYRCC 5 cut(s) 164, 272, 383, 626, 971
AccBSI CCGCTC 2 cut(s) 80, 357
AccI GTMKAC 2 cut(s) 420, 495
AccII CGCG 4 cut(s) 150, 297, 500, 527
AclWI GGATC 1 cut(s) 167
AcoI YGGCCR 3 cut(s) 99, 193, 771
AcsI RAATTY 1 cut(s) 867
AcuI CTGAAG 1 cut(s) 833
AcyI GRCGYC 5 cut(s) 273, 318, 351, 384, 411
AdeI CACNNNGTG 1 cut(s) 853
AfeI AGCGCT 1 cut(s) 981
AfiI CCNNNNNNNGG 8 cut(s) 97, 247, 380, 509, 576, 637, 638, 780
AgsI TTSAA 5 cut(s) 232, 681, 751, 877, 1022
AjnI CCWGG 2 cut(s) 693, 779
AleI CACNNNNGTG 1 cut(s) 149
AluBI AGCT 4 cut(s) 583, 655, 803, 931
AluI AGCT 4 cut(s) 583, 655, 803, 931
Alw26I GTCTC 1 cut(s) 510
AlwI GGATC 1 cut(s) 167
Ama87I CYCGRG 1 cut(s) 632
Aor51HI AGCGCT 1 cut(s) 981
AoxI GGCC 9 cut(s) 99, 178, 193, 249, 394, 640, 691, 739, 771
ApeKI GCWGC 1 cut(s) 928
ApoI RAATTY 1 cut(s) 867
AspLEI GCGC 7 cut(s) 275, 299, 386, 502, 527, 982, 1002
AspS9I GGNCC 8 cut(s) 178, 183, 250, 537, 640, 691, 739, 777
AsuC2I CCSGG 6 cut(s) 405, 633, 634, 775, 831, 909
AsuHPI GGTGA 5 cut(s) 21, 67, 214, 499, 968
AsuNHI GCTAGC 1 cut(s) 655
AvaI CYCGRG 1 cut(s) 632
AvaII GGWCC 3 cut(s) 183, 537, 777
BaeGI GKGCMC 2 cut(s) 631, 974
BanI GGYRCC 5 cut(s) 164, 272, 383, 626, 971
BanII GRGCYC 2 cut(s) 126, 441
BbsI GAAGAC 1 cut(s) 316
BbvI GCAGC 1 cut(s) 940
BccI CCATC 5 cut(s) 63, 145, 233, 338, 677
BceAI ACGGC 3 cut(s) 284, 434, 554
BciT130I CCWGG 2 cut(s) 695, 781
BcnI CCSGG 6 cut(s) 405, 633, 634, 775, 831, 909
BcoDI GTCTC 1 cut(s) 510
BfaI CTAG 1 cut(s) 656
BfoI RGCGCY 4 cut(s) 276, 387, 983, 1003
BglI GCCNNNNNGGC 2 cut(s) 408, 722
BglII AGATCT 1 cut(s) 456
BlpI GCTNAGC 1 cut(s) 924
Bme1390I CCNGG 8 cut(s) 405, 633, 634, 695, 775, 781, 831, 909
Bme18I GGWCC 3 cut(s) 183, 537, 777
BmeT110I CYCGRG 1 cut(s) 632
BmgT120I GGNCC 8 cut(s) 178, 183, 250, 537, 640, 691, 739, 777
BmiI GGNNCC 7 cut(s) 166, 274, 385, 538, 628, 778, 973
BmrFI CCNGG 8 cut(s) 405, 633, 634, 695, 775, 781, 831, 909
BmtI GCTAGC 1 cut(s) 659
BpiI GAAGAC 1 cut(s) 316
BpmI CTGGAG 1 cut(s) 1016
Bpu10I CCTNAGC 1 cut(s) 541
Bpu1102I GCTNAGC 1 cut(s) 924
BpuEI CTTGAG 1 cut(s) 929
BpuMI CCSGG 6 cut(s) 405, 633, 634, 775, 831, 909
BsaHI GRCGYC 5 cut(s) 273, 318, 351, 384, 411
BsaJI CCNNGG 7 cut(s) 96, 246, 403, 489, 631, 632, 908
BsaWI WCCGGW 1 cut(s) 167
BsaXI ACNNNNNCTCC 8 cut(s) 21, 50, 51, 80, 341, 362, 371, 392
Bsc4I CCNNNNNNNGG 8 cut(s) 97, 247, 380, 509, 576, 637, 638, 780
Bse118I RCCGGY 3 cut(s) 180, 209, 509
Bse1I ACTGG 1 cut(s) 643
BseBI CCWGG 2 cut(s) 695, 781
BseDI CCNNGG 7 cut(s) 96, 246, 403, 489, 631, 632, 908
BseLI CCNNNNNNNGG 8 cut(s) 97, 247, 380, 509, 576, 637, 638, 780
BseMII CTCAG 1 cut(s) 938
BseNI ACTGG 1 cut(s) 643
BseRI GAGGAG 5 cut(s) 74, 103, 191, 356, 478
BseSI GKGCMC 2 cut(s) 631, 974
BseX3I CGGCCG 1 cut(s) 771
BseXI GCAGC 1 cut(s) 940
BseYI CCCAGC 2 cut(s) 252, 725
Bsh1236I CGCG 4 cut(s) 150, 297, 500, 527
Bsh1285I CGRYCG 3 cut(s) 495, 765, 774
BshFI GGCC 9 cut(s) 101, 180, 195, 251, 396, 642, 693, 741, 773
BshNI GGYRCC 5 cut(s) 164, 272, 383, 626, 971
BsiEI CGRYCG 3 cut(s) 495, 765, 774
BsiHKCI CYCGRG 1 cut(s) 632
BslFI GGGAC 1 cut(s) 550
BslI CCNNNNNNNGG 8 cut(s) 97, 247, 380, 509, 576, 637, 638, 780
BsmAI GTCTC 1 cut(s) 510
BsmBI CGTCTC 1 cut(s) 510
BsmFI GGGAC 1 cut(s) 550
BsnI GGCC 9 cut(s) 101, 180, 195, 251, 396, 642, 693, 741, 773
BsoBI CYCGRG 1 cut(s) 632
Bsp1286I GDGCHC 4 cut(s) 126, 441, 631, 974
Bsp143I GATC 3 cut(s) 3, 159, 456
Bsp1720I GCTNAGC 1 cut(s) 924
Bsp19I CCATGG 1 cut(s) 96
BspANI GGCC 9 cut(s) 101, 180, 195, 251, 396, 642, 693, 741, 773
BspCNI CTCAG 1 cut(s) 937
BspFNI CGCG 4 cut(s) 150, 297, 500, 527
BspLI GGNNCC 7 cut(s) 166, 274, 385, 538, 628, 778, 973
BspOI GCTAGC 1 cut(s) 659
BspPI GGATC 1 cut(s) 167
BspT107I GGYRCC 5 cut(s) 164, 272, 383, 626, 971
BsrBI CCGCTC 2 cut(s) 80, 357
BsrFI RCCGGY 3 cut(s) 180, 209, 509
BsrI ACTGG 1 cut(s) 643
BssAI RCCGGY 3 cut(s) 180, 209, 509
BssECI CCNNGG 7 cut(s) 96, 246, 403, 489, 631, 632, 908
BssMI GATC 3 cut(s) 3, 159, 456
BssNI GRCGYC 5 cut(s) 273, 318, 351, 384, 411
BssT1I CCWWGG 2 cut(s) 96, 246
Bst2UI CCWGG 2 cut(s) 695, 781
Bst4CI ACNGT 1 cut(s) 1029
Bst6I CTCTTC 1 cut(s) 809
BstACI GRCGYC 5 cut(s) 273, 318, 351, 384, 411
BstC8I GCNNGC 4 cut(s) 211, 556, 657, 900
BstDEI CTNAG 3 cut(s) 541, 804, 924
BstDSI CCRYGG 1 cut(s) 96
BstENI CCTNNNNNAGG 1 cut(s) 245
BstFNI CGCG 4 cut(s) 150, 297, 500, 527
BstH2I RGCGCY 4 cut(s) 276, 387, 983, 1003
BstHHI GCGC 7 cut(s) 275, 299, 386, 502, 527, 982, 1002
BstKTI GATC 3 cut(s) 6, 162, 459
BstMAI GTCTC 1 cut(s) 510
BstMBI GATC 3 cut(s) 3, 159, 456
BstMCI CGRYCG 3 cut(s) 495, 765, 774
BstMWI GCNNNNNNNGC 9 cut(s) 107, 162, 281, 408, 551, 656, 722, 904, 969
BstNI CCWGG 2 cut(s) 695, 781
BstSCI CCNGG 8 cut(s) 403, 631, 632, 693, 773, 779, 829, 907
BstSLI GKGCMC 2 cut(s) 631, 974
BstUI CGCG 4 cut(s) 150, 297, 500, 527
BstV1I GCAGC 1 cut(s) 940
BstV2I GAAGAC 1 cut(s) 316
BstX2I RGATCY 1 cut(s) 456
BstXI CCANNNNNNTGG 3 cut(s) 151, 725, 787
BstYI RGATCY 1 cut(s) 456
BstZI CGGCCG 1 cut(s) 771
BsuRI GGCC 9 cut(s) 101, 180, 195, 251, 396, 642, 693, 741, 773
BtgI CCRYGG 1 cut(s) 96
Cac8I GCNNGC 4 cut(s) 211, 556, 657, 900
CfoI GCGC 7 cut(s) 275, 299, 386, 502, 527, 982, 1002
Cfr10I RCCGGY 3 cut(s) 180, 209, 509
Cfr13I GGNCC 8 cut(s) 178, 183, 250, 537, 640, 691, 739, 777
Cfr9I CCCGGG 1 cut(s) 632
CseI GACGC 2 cut(s) 400, 506
CviAII CATG 8 cut(s) 97, 175, 187, 424, 622, 706, 892, 953
DdeI CTNAG 3 cut(s) 541, 804, 924
DinI GGCGCC 2 cut(s) 274, 385
DpnI GATC 3 cut(s) 5, 161, 458
DpnII GATC 3 cut(s) 3, 159, 456
DraIII CACNNNGTG 1 cut(s) 853
DrdI GACNNNNNNGTC 2 cut(s) 503, 569
DseDI GACNNNNNNGTC 2 cut(s) 503, 569
EaeI YGGCCR 3 cut(s) 99, 193, 771
EagI CGGCCG 1 cut(s) 771
Eam1104I CTCTTC 1 cut(s) 809
EarI CTCTTC 1 cut(s) 809
EciI GGCGGA 4 cut(s) 77, 389, 457, 528
EclXI CGGCCG 1 cut(s) 771
Eco130I CCWWGG 2 cut(s) 96, 246
Eco24I GRGCYC 2 cut(s) 126, 441
Eco47I GGWCC 3 cut(s) 183, 537, 777
Eco47III AGCGCT 1 cut(s) 981
Eco52I CGGCCG 1 cut(s) 771
Eco57I CTGAAG 1 cut(s) 833
Eco88I CYCGRG 1 cut(s) 632
EcoNI CCTNNNNNAGG 1 cut(s) 245
EcoO109I RGGNCCY 1 cut(s) 537
EcoRII CCWGG 2 cut(s) 693, 779
EcoT14I CCWWGG 2 cut(s) 96, 246
EcoT38I GRGCYC 2 cut(s) 126, 441
EgeI GGCGCC 2 cut(s) 274, 385
EheI GGCGCC 2 cut(s) 274, 385
ErhI CCWWGG 2 cut(s) 96, 246
Esp3I CGTCTC 1 cut(s) 510
FaeI CATG 8 cut(s) 100, 178, 190, 427, 625, 709, 895, 956
FaqI GGGAC 1 cut(s) 550
FatI CATG 8 cut(s) 96, 174, 186, 423, 621, 705, 891, 952
FauI CCCGC 2 cut(s) 155, 547
FauNDI CATATG 1 cut(s) 674
FblI GTMKAC 2 cut(s) 420, 495
FriOI GRGCYC 2 cut(s) 126, 441
FspBI CTAG 1 cut(s) 656
GlaI GCGC 7 cut(s) 274, 298, 385, 501, 526, 981, 1001
GsaI CCCAGC 2 cut(s) 256, 729
GsuI CTGGAG 1 cut(s) 1016
HaeII RGCGCY 4 cut(s) 276, 387, 983, 1003
HaeIII GGCC 9 cut(s) 101, 180, 195, 251, 396, 642, 693, 741, 773
HgaI GACGC 2 cut(s) 400, 506
HhaI GCGC 7 cut(s) 275, 299, 386, 502, 527, 982, 1002
Hin1I GRCGYC 5 cut(s) 273, 318, 351, 384, 411
Hin1II CATG 8 cut(s) 100, 178, 190, 427, 625, 709, 895, 956
Hin6I GCGC 7 cut(s) 273, 297, 384, 500, 525, 980, 1000
HinP1I GCGC 7 cut(s) 273, 297, 384, 500, 525, 980, 1000
HincII GTYRAC 3 cut(s) 421, 496, 1038
HindII GTYRAC 3 cut(s) 421, 496, 1038
HinfI GANTC 7 cut(s) 171, 287, 547, 586, 808, 834, 844
HphI GGTGA 5 cut(s) 21, 67, 214, 499, 968
Hpy166II GTNNAC 3 cut(s) 421, 496, 1038
Hpy188I TCNGA 5 cut(s) 316, 349, 436, 813, 888
Hpy188III TCNNGA 4 cut(s) 263, 454, 748, 995
Hpy8I GTNNAC 3 cut(s) 421, 496, 1038
Hpy99I CGWCG 9 cut(s) 305, 320, 323, 338, 353, 455, 500, 566, 575
HpyAV CCTTC 6 cut(s) 50, 631, 695, 745, 850, 871
HpyCH4III ACNGT 1 cut(s) 1029
HpyCH4IV ACGT 3 cut(s) 318, 351, 519
HpyCH4V TGCA 2 cut(s) 650, 796
HpyF10VI GCNNNNNNNGC 9 cut(s) 107, 162, 281, 408, 551, 656, 722, 904, 969
HpyF3I CTNAG 3 cut(s) 541, 804, 924
HpySE526I ACGT 3 cut(s) 318, 351, 519
Hsp92I GRCGYC 5 cut(s) 273, 318, 351, 384, 411
Hsp92II CATG 8 cut(s) 100, 178, 190, 427, 625, 709, 895, 956
HspAI GCGC 7 cut(s) 273, 297, 384, 500, 525, 980, 1000
KasI GGCGCC 2 cut(s) 272, 383
KroI GCCGGC 1 cut(s) 209
KroNI GCCGGC 1 cut(s) 211
Kzo9I GATC 3 cut(s) 3, 159, 456
LmnI GCTCC 4 cut(s) 362, 491, 800, 997
Lsp1109I GCAGC 1 cut(s) 940
MaeI CTAG 1 cut(s) 656
MaeII ACGT 3 cut(s) 318, 351, 519
MaeIII GTNAC 3 cut(s) 505, 939, 1014
MalI GATC 3 cut(s) 5, 161, 458
MbiI CCGCTC 2 cut(s) 80, 357
MboI GATC 3 cut(s) 3, 159, 456
MboII GAAGA 3 cut(s) 220, 316, 826
MflI RGATCY 1 cut(s) 456
MhlI GDGCHC 4 cut(s) 126, 441, 631, 974
MluCI AATT 3 cut(s) 616, 867, 964
Mly113I GGCGCC 2 cut(s) 273, 384
MlyI GAGTC 1 cut(s) 180
MmeI TCCRAC 3 cut(s) 339, 372, 768
MroNI GCCGGC 1 cut(s) 209
MseI TTAA 1 cut(s) 615
MslI CAYNNNNRTG 1 cut(s) 149
MspR9I CCNGG 8 cut(s) 405, 633, 634, 695, 775, 781, 831, 909
MvaI CCWGG 2 cut(s) 695, 781
MvnI CGCG 4 cut(s) 150, 297, 500, 527
MwoI GCNNNNNNNGC 9 cut(s) 107, 162, 281, 408, 551, 656, 722, 904, 969
NaeI GCCGGC 1 cut(s) 211
NarI GGCGCC 2 cut(s) 273, 384
NciI CCSGG 6 cut(s) 405, 633, 634, 775, 831, 909
NcoI CCATGG 1 cut(s) 96
NdeI CATATG 1 cut(s) 674
NdeII GATC 3 cut(s) 3, 159, 456
NgoMIV GCCGGC 1 cut(s) 209
NheI GCTAGC 1 cut(s) 655
NlaIII CATG 8 cut(s) 100, 178, 190, 427, 625, 709, 895, 956
NlaIV GGNNCC 7 cut(s) 166, 274, 385, 538, 628, 778, 973
NmuCI GTSAC 3 cut(s) 505, 939, 1014
OliI CACNNNNGTG 1 cut(s) 149
PcsI WCGNNNNNNNCGW 3 cut(s) 357, 501, 570
PdiI GCCGGC 1 cut(s) 211
PfeI GAWTC 6 cut(s) 287, 547, 586, 808, 834, 844
PflFI GACNNNGTC 1 cut(s) 320
PleI GAGTC 1 cut(s) 179
PluTI GGCGCC 2 cut(s) 276, 387
PpsI GAGTC 1 cut(s) 179
PpuMI RGGWCCY 1 cut(s) 537
Psp5II RGGWCCY 1 cut(s) 537
Psp6I CCWGG 2 cut(s) 693, 779
PspFI CCCAGC 2 cut(s) 252, 725
PspGI CCWGG 2 cut(s) 693, 779
PspN4I GGNNCC 7 cut(s) 166, 274, 385, 538, 628, 778, 973
PspPI GGNCC 8 cut(s) 178, 183, 250, 537, 640, 691, 739, 777
PspPPI RGGWCCY 1 cut(s) 537
PsuI RGATCY 1 cut(s) 456
PsyI GACNNNGTC 1 cut(s) 320
RseI CAYNNNNRTG 1 cut(s) 149
SalI GTCGAC 2 cut(s) 419, 494
SaqAI TTAA 1 cut(s) 615
Sau3AI GATC 3 cut(s) 3, 159, 456
Sau96I GGNCC 8 cut(s) 178, 183, 250, 537, 640, 691, 739, 777
SchI GAGTC 1 cut(s) 180
ScrFI CCNGG 8 cut(s) 405, 633, 634, 695, 775, 781, 831, 909
SduI GDGCHC 4 cut(s) 126, 441, 631, 974
SfoI GGCGCC 2 cut(s) 274, 385
SgrAI CRCCGGYG 1 cut(s) 509
SinI GGWCC 3 cut(s) 183, 537, 777
SmaI CCCGGG 1 cut(s) 634
SmiMI CAYNNNNRTG 1 cut(s) 149
SmlI CTYRAG 1 cut(s) 944
SmoI CTYRAG 1 cut(s) 944
Sse9I AATT 3 cut(s) 616, 867, 964
SspDI GGCGCC 2 cut(s) 272, 383
SspMI CTAG 1 cut(s) 656
StyD4I CCNGG 8 cut(s) 403, 631, 632, 693, 773, 779, 829, 907
StyI CCWWGG 2 cut(s) 96, 246
TaaI ACNGT 1 cut(s) 1029
TaiI ACGT 3 cut(s) 321, 354, 522
TaqI TCGA 6 cut(s) 264, 369, 420, 453, 495, 825
TaqII GACCGA 2 cut(s) 481, 751
TasI AATT 3 cut(s) 616, 867, 964
TfiI GAWTC 6 cut(s) 287, 547, 586, 808, 834, 844
Tru1I TTAA 1 cut(s) 615
Tru9I TTAA 1 cut(s) 615
TseFI GTSAC 3 cut(s) 505, 939, 1014
TseI GCWGC 1 cut(s) 928
Tsp45I GTSAC 3 cut(s) 505, 939, 1014
TspGWI ACGGA 2 cut(s) 349, 813
TspMI CCCGGG 1 cut(s) 632
Tth111I GACNNNGTC 1 cut(s) 320
VpaK11BI GGWCC 3 cut(s) 183, 537, 777
XagI CCTNNNNNAGG 1 cut(s) 245
XapI RAATTY 1 cut(s) 867
XmaI CCCGGG 1 cut(s) 632
XmiI GTMKAC 2 cut(s) 420, 495
XspI CTAG 1 cut(s) 656
ZraI GACGTC 2 cut(s) 319, 352
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.