Rmu_sc0009216.1_g000005

3-hydroxyisobutyrate dehydrogenase-like 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0009216.1
OG0016018
Physical Location & Seq
Reverse (-)
14704 .. 15750
1047 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0009216.1_g000005.1.cds

Sequence Viewer

Length: 1047 bp
atgatcctcaaaatagtcaaacacatttcacctccactccttccccaactctcccatcacttccactcccacttcaccgctctcctccgccgcaccatggccgccgcctcctccaccgagcccactattagtccatcaaccacccgcgtgggctggatcggcaccggagtcatgggccggtccatgtgcggccacctcctcaaagccggctacactctcaccatcttcaaccgcaccctatccaaggcccagcctcttctcgatttgggcgccaaactcgccgattccccacgcgccgtcgcctcccaatccgacgtcgtcttctccatcgtcggctacccttccgatgtccgctccgtcctcctcgaccccacctccggcgccctctccggcctccgccccggcggcgtcctcgtcgacatgaccacctccgagccttccctcgccgtcgagatctcccaatccgccgccgccaagtcctgctcctcgatcgacgcgcctgtcaccggcggagacgtcggcgcgaaaaacgggaccttagcgattctggcgggcggcgacgaagccgtcgtggggaagctgattcccctttttgccctcatgggtaaggttaattacatgggtgcccccgggaagggccagtttgcaaagctagcgaaccagaccatcatatgttcaacaatgttgggcctggtagaaggcatggtttttgcccacaaggctgggcttgatgtgggcctgttcttgaaggcaatctcggtcggggcggccgggtccaggtcgttggatttgcatggagctaagattctgaagagggatttcgagccgggattctatgtgaatcactttgtgaaggacttggggatttgtttgaaggagtgccagaacatggggctggctctgccggggttggctctggctcagcagctttacttgtcactcaaggctcatggtgagggcaatttgggcacccaagcgctcattgtggctctggagcgcctcaacaatgtgtcacttgaacaacagtctgaaccgacaaagaagtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAD_binding_11 NAD_binding_2

Protein Analysis

348

Amino Acids

36.37

Weight (kDa)

9.15

Isoelectric Point (pI)

36.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016018)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G29120
fragaria_vesca FvH4_1g06170
malus_domestica MD02G1065900.v1.1 MD02G1066000.v1.1
prunus_persica Prupe.7G219600_v2.0.a1
pyrus_communis pycom02g05230
rosa_chinensis RchiOBHm_Chr2g0091951
rosa_laevigata RLG00000016265
rosa_multiflora Rmu_sc0009216.1_g000005 Rmu_sc0017125.1_g000007
rosa_roxburghii Rroxscaffold_2G00149590
rosa_rugosa Rorug02G0021800
rosa_samantha Rh2AG067400 Rh2DG066500
rosa_wichuraiana Rw2G005360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 500, 566
AatII GACGTC 2 cut(s) 318, 519
AccB1I GGYRCC 5 cut(s) 161, 269, 380, 623, 968
AccB7I CCANNNNNTGG 1 cut(s) 889
AccBSI CCGCTC 2 cut(s) 80, 354
AccI GTMKAC 1 cut(s) 417
AccII CGCG 4 cut(s) 147, 294, 497, 524
AclWI GGATC 1 cut(s) 164
AcoI YGGCCR 3 cut(s) 99, 190, 768
AcuI CTGAAG 1 cut(s) 830
AcyI GRCGYC 5 cut(s) 270, 315, 381, 408, 516
AdeI CACNNNGTG 1 cut(s) 850
AfeI AGCGCT 1 cut(s) 978
AfiI CCNNNNNNNGG 9 cut(s) 97, 244, 377, 506, 573, 634, 635, 777, 889
AgsI TTSAA 5 cut(s) 229, 678, 748, 874, 1019
AjnI CCWGG 2 cut(s) 690, 776
AleI CACNNNNGTG 1 cut(s) 146
AluBI AGCT 4 cut(s) 580, 652, 800, 928
AluI AGCT 4 cut(s) 580, 652, 800, 928
Alw26I GTCTC 1 cut(s) 507
AlwI GGATC 1 cut(s) 164
Ama87I CYCGRG 1 cut(s) 629
Aor51HI AGCGCT 1 cut(s) 978
AoxI GGCC 9 cut(s) 99, 175, 190, 246, 391, 637, 688, 736, 768
ApeKI GCWGC 1 cut(s) 925
AspLEI GCGC 7 cut(s) 272, 296, 383, 499, 524, 979, 999
AspS9I GGNCC 8 cut(s) 175, 180, 247, 534, 637, 688, 736, 774
AsuC2I CCSGG 6 cut(s) 402, 630, 631, 772, 828, 906
AsuHPI GGTGA 5 cut(s) 21, 67, 211, 496, 965
AsuNHI GCTAGC 1 cut(s) 652
AvaI CYCGRG 1 cut(s) 629
AvaII GGWCC 3 cut(s) 180, 534, 774
BaeGI GKGCMC 2 cut(s) 628, 971
BanI GGYRCC 5 cut(s) 161, 269, 380, 623, 968
BanII GRGCYC 1 cut(s) 123
BbsI GAAGAC 1 cut(s) 313
BbvI GCAGC 1 cut(s) 937
BccI CCATC 5 cut(s) 63, 142, 230, 335, 674
BceAI ACGGC 3 cut(s) 281, 431, 551
BciT130I CCWGG 2 cut(s) 692, 778
BcnI CCSGG 6 cut(s) 402, 630, 631, 772, 828, 906
BcoDI GTCTC 1 cut(s) 507
BfaI CTAG 1 cut(s) 653
BfoI RGCGCY 4 cut(s) 273, 384, 980, 1000
BglI GCCNNNNNGGC 2 cut(s) 405, 719
BglII AGATCT 1 cut(s) 453
BlpI GCTNAGC 1 cut(s) 921
Bme1390I CCNGG 8 cut(s) 402, 630, 631, 692, 772, 778, 828, 906
Bme18I GGWCC 3 cut(s) 180, 534, 774
BmeT110I CYCGRG 1 cut(s) 629
BmgT120I GGNCC 8 cut(s) 175, 180, 247, 534, 637, 688, 736, 774
BmiI GGNNCC 7 cut(s) 163, 271, 382, 535, 625, 775, 970
BmrFI CCNGG 8 cut(s) 402, 630, 631, 692, 772, 778, 828, 906
BmtI GCTAGC 1 cut(s) 656
BpiI GAAGAC 1 cut(s) 313
BpmI CTGGAG 1 cut(s) 1013
Bpu10I CCTNAGC 1 cut(s) 538
Bpu1102I GCTNAGC 1 cut(s) 921
BpuEI CTTGAG 1 cut(s) 926
BpuMI CCSGG 6 cut(s) 402, 630, 631, 772, 828, 906
BsaHI GRCGYC 5 cut(s) 270, 315, 381, 408, 516
BsaJI CCNNGG 6 cut(s) 96, 243, 400, 628, 629, 905
BsaWI WCCGGW 1 cut(s) 164
BsaXI ACNNNNNCTCC 6 cut(s) 21, 50, 51, 80, 359, 389
Bsc4I CCNNNNNNNGG 9 cut(s) 97, 244, 377, 506, 573, 634, 635, 777, 889
Bse118I RCCGGY 3 cut(s) 177, 206, 506
Bse1I ACTGG 1 cut(s) 640
BseBI CCWGG 2 cut(s) 692, 778
BseDI CCNNGG 6 cut(s) 96, 243, 400, 628, 629, 905
BseLI CCNNNNNNNGG 9 cut(s) 97, 244, 377, 506, 573, 634, 635, 777, 889
BseMII CTCAG 1 cut(s) 935
BseNI ACTGG 1 cut(s) 640
BseRI GAGGAG 5 cut(s) 74, 100, 188, 353, 475
BseSI GKGCMC 2 cut(s) 628, 971
BseX3I CGGCCG 1 cut(s) 768
BseXI GCAGC 1 cut(s) 937
BseYI CCCAGC 2 cut(s) 249, 722
Bsh1236I CGCG 4 cut(s) 147, 294, 497, 524
Bsh1285I CGRYCG 3 cut(s) 492, 762, 771
BshFI GGCC 9 cut(s) 101, 177, 192, 248, 393, 639, 690, 738, 770
BshNI GGYRCC 5 cut(s) 161, 269, 380, 623, 968
BsiEI CGRYCG 3 cut(s) 492, 762, 771
BsiHKCI CYCGRG 1 cut(s) 629
BslFI GGGAC 1 cut(s) 547
BslI CCNNNNNNNGG 9 cut(s) 97, 244, 377, 506, 573, 634, 635, 777, 889
BsmAI GTCTC 1 cut(s) 507
BsmBI CGTCTC 1 cut(s) 507
BsmFI GGGAC 1 cut(s) 547
BsnI GGCC 9 cut(s) 101, 177, 192, 248, 393, 639, 690, 738, 770
BsoBI CYCGRG 1 cut(s) 629
Bsp1286I GDGCHC 3 cut(s) 123, 628, 971
Bsp143I GATC 4 cut(s) 3, 156, 453, 489
Bsp1720I GCTNAGC 1 cut(s) 921
Bsp19I CCATGG 1 cut(s) 96
BspANI GGCC 9 cut(s) 101, 177, 192, 248, 393, 639, 690, 738, 770
BspCNI CTCAG 1 cut(s) 934
BspFNI CGCG 4 cut(s) 147, 294, 497, 524
BspLI GGNNCC 7 cut(s) 163, 271, 382, 535, 625, 775, 970
BspOI GCTAGC 1 cut(s) 656
BspPI GGATC 1 cut(s) 164
BspT107I GGYRCC 5 cut(s) 161, 269, 380, 623, 968
BsrBI CCGCTC 2 cut(s) 80, 354
BsrFI RCCGGY 3 cut(s) 177, 206, 506
BsrI ACTGG 1 cut(s) 640
BssAI RCCGGY 3 cut(s) 177, 206, 506
BssECI CCNNGG 6 cut(s) 96, 243, 400, 628, 629, 905
BssMI GATC 4 cut(s) 3, 156, 453, 489
BssNI GRCGYC 5 cut(s) 270, 315, 381, 408, 516
BssT1I CCWWGG 2 cut(s) 96, 243
Bst2UI CCWGG 2 cut(s) 692, 778
Bst4CI ACNGT 1 cut(s) 1026
Bst6I CTCTTC 2 cut(s) 261, 806
BstACI GRCGYC 5 cut(s) 270, 315, 381, 408, 516
BstC8I GCNNGC 4 cut(s) 208, 553, 654, 897
BstDEI CTNAG 3 cut(s) 538, 801, 921
BstDSI CCRYGG 1 cut(s) 96
BstENI CCTNNNNNAGG 1 cut(s) 242
BstFNI CGCG 4 cut(s) 147, 294, 497, 524
BstH2I RGCGCY 4 cut(s) 273, 384, 980, 1000
BstHHI GCGC 7 cut(s) 272, 296, 383, 499, 524, 979, 999
BstKTI GATC 4 cut(s) 6, 159, 456, 492
BstMAI GTCTC 1 cut(s) 507
BstMBI GATC 4 cut(s) 3, 156, 453, 489
BstMCI CGRYCG 3 cut(s) 492, 762, 771
BstMWI GCNNNNNNNGC 8 cut(s) 159, 278, 405, 548, 653, 719, 901, 966
BstNI CCWGG 2 cut(s) 692, 778
BstSCI CCNGG 8 cut(s) 400, 628, 629, 690, 770, 776, 826, 904
BstSLI GKGCMC 2 cut(s) 628, 971
BstUI CGCG 4 cut(s) 147, 294, 497, 524
BstV1I GCAGC 1 cut(s) 937
BstV2I GAAGAC 1 cut(s) 313
BstX2I RGATCY 1 cut(s) 453
BstXI CCANNNNNNTGG 3 cut(s) 148, 722, 784
BstYI RGATCY 1 cut(s) 453
BstZI CGGCCG 1 cut(s) 768
BsuRI GGCC 9 cut(s) 101, 177, 192, 248, 393, 639, 690, 738, 770
BtgI CCRYGG 1 cut(s) 96
Cac8I GCNNGC 4 cut(s) 208, 553, 654, 897
CfoI GCGC 7 cut(s) 272, 296, 383, 499, 524, 979, 999
Cfr10I RCCGGY 3 cut(s) 177, 206, 506
Cfr13I GGNCC 8 cut(s) 175, 180, 247, 534, 637, 688, 736, 774
Cfr9I CCCGGG 1 cut(s) 629
CseI GACGC 2 cut(s) 397, 503
DdeI CTNAG 3 cut(s) 538, 801, 921
DinI GGCGCC 2 cut(s) 271, 382
DpnI GATC 4 cut(s) 5, 158, 455, 491
DpnII GATC 4 cut(s) 3, 156, 453, 489
DraIII CACNNNGTG 1 cut(s) 850
DrdI GACNNNNNNGTC 2 cut(s) 500, 566
DseDI GACNNNNNNGTC 2 cut(s) 500, 566
EaeI YGGCCR 3 cut(s) 99, 190, 768
EagI CGGCCG 1 cut(s) 768
Eam1104I CTCTTC 2 cut(s) 261, 806
EarI CTCTTC 2 cut(s) 261, 806
EciI GGCGGA 4 cut(s) 77, 386, 454, 525
EclXI CGGCCG 1 cut(s) 768
Eco130I CCWWGG 2 cut(s) 96, 243
Eco24I GRGCYC 1 cut(s) 123
Eco47I GGWCC 3 cut(s) 180, 534, 774
Eco47III AGCGCT 1 cut(s) 978
Eco52I CGGCCG 1 cut(s) 768
Eco57I CTGAAG 1 cut(s) 830
Eco88I CYCGRG 1 cut(s) 629
EcoNI CCTNNNNNAGG 1 cut(s) 242
EcoO109I RGGNCCY 1 cut(s) 534
EcoRII CCWGG 2 cut(s) 690, 776
EcoT14I CCWWGG 2 cut(s) 96, 243
EcoT38I GRGCYC 1 cut(s) 123
EgeI GGCGCC 2 cut(s) 271, 382
EheI GGCGCC 2 cut(s) 271, 382
ErhI CCWWGG 2 cut(s) 96, 243
Esp3I CGTCTC 1 cut(s) 507
FaqI GGGAC 1 cut(s) 547
FauI CCCGC 2 cut(s) 152, 544
FauNDI CATATG 1 cut(s) 671
FblI GTMKAC 1 cut(s) 417
FriOI GRGCYC 1 cut(s) 123
FspBI CTAG 1 cut(s) 653
GlaI GCGC 7 cut(s) 271, 295, 382, 498, 523, 978, 998
GsaI CCCAGC 2 cut(s) 253, 726
GsuI CTGGAG 1 cut(s) 1013
HaeII RGCGCY 4 cut(s) 273, 384, 980, 1000
HaeIII GGCC 9 cut(s) 101, 177, 192, 248, 393, 639, 690, 738, 770
HgaI GACGC 2 cut(s) 397, 503
HhaI GCGC 7 cut(s) 272, 296, 383, 499, 524, 979, 999
Hin1I GRCGYC 5 cut(s) 270, 315, 381, 408, 516
Hin6I GCGC 7 cut(s) 270, 294, 381, 497, 522, 977, 997
HinP1I GCGC 7 cut(s) 270, 294, 381, 497, 522, 977, 997
HincII GTYRAC 1 cut(s) 418
HindII GTYRAC 1 cut(s) 418
HinfI GANTC 7 cut(s) 168, 284, 544, 583, 805, 831, 841
HphI GGTGA 5 cut(s) 21, 67, 211, 496, 965
Hpy166II GTNNAC 1 cut(s) 418
Hpy188I TCNGA 5 cut(s) 313, 346, 433, 810, 1030
Hpy188III TCNNGA 4 cut(s) 260, 451, 745, 992
Hpy8I GTNNAC 1 cut(s) 418
HpyAV CCTTC 8 cut(s) 50, 351, 447, 628, 692, 742, 847, 868
HpyCH4III ACNGT 1 cut(s) 1026
HpyCH4IV ACGT 2 cut(s) 315, 516
HpyCH4V TGCA 2 cut(s) 647, 793
HpyF10VI GCNNNNNNNGC 8 cut(s) 159, 278, 405, 548, 653, 719, 901, 966
HpyF3I CTNAG 3 cut(s) 538, 801, 921
HpySE526I ACGT 2 cut(s) 315, 516
Hsp92I GRCGYC 5 cut(s) 270, 315, 381, 408, 516
HspAI GCGC 7 cut(s) 270, 294, 381, 497, 522, 977, 997
KasI GGCGCC 2 cut(s) 269, 380
KroI GCCGGC 1 cut(s) 206
KroNI GCCGGC 1 cut(s) 208
Kzo9I GATC 4 cut(s) 3, 156, 453, 489
LmnI GCTCC 4 cut(s) 359, 488, 797, 994
Lsp1109I GCAGC 1 cut(s) 937
MaeI CTAG 1 cut(s) 653
MaeII ACGT 2 cut(s) 315, 516
MaeIII GTNAC 3 cut(s) 502, 936, 1011
MalI GATC 4 cut(s) 5, 158, 455, 491
MbiI CCGCTC 2 cut(s) 80, 354
MboI GATC 4 cut(s) 3, 156, 453, 489
MboII GAAGA 4 cut(s) 217, 248, 313, 823
MflI RGATCY 1 cut(s) 453
MhlI GDGCHC 3 cut(s) 123, 628, 971
MluCI AATT 2 cut(s) 613, 961
Mly113I GGCGCC 2 cut(s) 270, 381
MlyI GAGTC 1 cut(s) 177
MmeI TCCRAC 2 cut(s) 336, 765
MroNI GCCGGC 1 cut(s) 206
MseI TTAA 1 cut(s) 612
MslI CAYNNNNRTG 1 cut(s) 146
MspR9I CCNGG 8 cut(s) 402, 630, 631, 692, 772, 778, 828, 906
MvaI CCWGG 2 cut(s) 692, 778
MvnI CGCG 4 cut(s) 147, 294, 497, 524
MwoI GCNNNNNNNGC 8 cut(s) 159, 278, 405, 548, 653, 719, 901, 966
NaeI GCCGGC 1 cut(s) 208
NarI GGCGCC 2 cut(s) 270, 381
NciI CCSGG 6 cut(s) 402, 630, 631, 772, 828, 906
NcoI CCATGG 1 cut(s) 96
NdeI CATATG 1 cut(s) 671
NdeII GATC 4 cut(s) 3, 156, 453, 489
NgoMIV GCCGGC 1 cut(s) 206
NheI GCTAGC 1 cut(s) 652
NlaIV GGNNCC 7 cut(s) 163, 271, 382, 535, 625, 775, 970
NmuCI GTSAC 3 cut(s) 502, 936, 1011
OliI CACNNNNGTG 1 cut(s) 146
PcsI WCGNNNNNNNCGW 1 cut(s) 567
PdiI GCCGGC 1 cut(s) 208
PfeI GAWTC 6 cut(s) 284, 544, 583, 805, 831, 841
PflFI GACNNNGTC 1 cut(s) 317
PflMI CCANNNNNTGG 1 cut(s) 889
Ple19I CGATCG 1 cut(s) 492
PleI GAGTC 1 cut(s) 176
PluTI GGCGCC 2 cut(s) 273, 384
PpsI GAGTC 1 cut(s) 176
PpuMI RGGWCCY 1 cut(s) 534
Psp5II RGGWCCY 1 cut(s) 534
Psp6I CCWGG 2 cut(s) 690, 776
PspFI CCCAGC 2 cut(s) 249, 722
PspGI CCWGG 2 cut(s) 690, 776
PspN4I GGNNCC 7 cut(s) 163, 271, 382, 535, 625, 775, 970
PspPI GGNCC 8 cut(s) 175, 180, 247, 534, 637, 688, 736, 774
PspPPI RGGWCCY 1 cut(s) 534
PsuI RGATCY 1 cut(s) 453
PsyI GACNNNGTC 1 cut(s) 317
PvuI CGATCG 1 cut(s) 492
RseI CAYNNNNRTG 1 cut(s) 146
SalI GTCGAC 1 cut(s) 416
SaqAI TTAA 1 cut(s) 612
Sau3AI GATC 4 cut(s) 3, 156, 453, 489
Sau96I GGNCC 8 cut(s) 175, 180, 247, 534, 637, 688, 736, 774
SchI GAGTC 1 cut(s) 177
ScrFI CCNGG 8 cut(s) 402, 630, 631, 692, 772, 778, 828, 906
SduI GDGCHC 3 cut(s) 123, 628, 971
SfoI GGCGCC 2 cut(s) 271, 382
SgrAI CRCCGGYG 1 cut(s) 506
SinI GGWCC 3 cut(s) 180, 534, 774
SmaI CCCGGG 1 cut(s) 631
SmiMI CAYNNNNRTG 1 cut(s) 146
SmlI CTYRAG 1 cut(s) 941
SmoI CTYRAG 1 cut(s) 941
Sse9I AATT 2 cut(s) 613, 961
SspDI GGCGCC 2 cut(s) 269, 380
SspMI CTAG 1 cut(s) 653
StyD4I CCNGG 8 cut(s) 400, 628, 629, 690, 770, 776, 826, 904
StyI CCWWGG 2 cut(s) 96, 243
TaaI ACNGT 1 cut(s) 1026
TaiI ACGT 2 cut(s) 318, 519
TaqI TCGA 7 cut(s) 261, 366, 417, 450, 488, 492, 822
TaqII GACCGA 1 cut(s) 748
TasI AATT 2 cut(s) 613, 961
TauI GCSGC 9 cut(s) 93, 104, 107, 192, 408, 470, 473, 558, 770
TfiI GAWTC 6 cut(s) 284, 544, 583, 805, 831, 841
Tru1I TTAA 1 cut(s) 612
Tru9I TTAA 1 cut(s) 612
TseFI GTSAC 3 cut(s) 502, 936, 1011
TseI GCWGC 1 cut(s) 925
Tsp45I GTSAC 3 cut(s) 502, 936, 1011
TspGWI ACGGA 1 cut(s) 346
TspMI CCCGGG 1 cut(s) 629
Tth111I GACNNNGTC 1 cut(s) 317
Van91I CCANNNNNTGG 1 cut(s) 889
VpaK11BI GGWCC 3 cut(s) 180, 534, 774
XagI CCTNNNNNAGG 1 cut(s) 242
XmaI CCCGGG 1 cut(s) 629
XmiI GTMKAC 1 cut(s) 417
XspI CTAG 1 cut(s) 653
ZraI GACGTC 2 cut(s) 316, 517
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.