RchiOBHm_Chr2g0091951

3-hydroxyisobutyrate dehydrogenase-like 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0016018
Physical Location & Seq
Forward (+)
5576567 .. 5577857
1291 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ46712

Sequence Viewer

Length: 1050 bp
ATGATCCTCAAAATAGTCAAACACATTTCACCTCCACTCCTTCCCCAACTCTCCCATCACTTCCACTCCCACTTCACCGCTCTCCTCCGCCGCACCATGGCCGCCGCCGCCGCCTCCTCCACCGAGCCCACTATAAGCCCATCAACCACCCGCGTGGGCTGGATCGGCACCGGAGTCATGGGCCGGTCCATGTGCGGCCATCTCCTCAAAGCCGGCTACACTCTCACCATCTTCAACCGCACCCTATCCAAGGCCCAGCCTCTTCTCGATTTGGGCGCCAAACTCGCCGATTCCCCACGCGCCGTCGCCTCCCAATCCGACGTCGTCTTCTCCATCGTCGGCTACCCCTCCGATGTCCGCTCCGTCCTCCTCGACCCCACCTCCGGCGCCCTCTCCGGCCTCCGCCCCGGCGGCGTCCTCGTCGACATGACCACCTCCGAGCCCTCCCTGGCCGTCGAGATCTCCCAATCCGCCGCCGCCAAGTCCTGCTCCTCGATCGACGCGCCCGTCACCGGCGGAGACGTCGGCGCGAAAAACGGGACCTTAGCGATTCTGGCGGGCGGCGACGAAGCCGTCGTGGGGAAGCTGATTCCCCTTTTTGCCCTCATGGGTAAGGTTAATTACATGGGTGCCCCCGGGAAGGGCCAGTTTGCAAAGCTAGCGAACCAGACCATCATATGTTCAACAATGTTGGGCCTGGTAGAAGGCATGGTTTTTGCCCACAAGGCTGGGCTTGATGTGGGCCTGTTCTTGAAGGCAATCTCGGTCGGGGCGGCCGGGTCCAGGTCGTTGGATTTGCATGGAGCTAAGATTCTGAAGAGGGATTTCGAGCCGGGATTCTATGTGAATCACTTTGTGAAGGACTTGGGGATTTGTTTGAAGGAGTGCCAGAACATGGGGCTGGCTCTGCCGGGGTTGGCTCTGGCTCAGCAGCTTTACTTGTCACTCAAGGCTCATGGTGAGGGCAATTTGGGCACCCAAGCGCTCATTGTGGCTCTGGAGCGCCTCAACAATGTGTCACTTGAACAACAGTCTGGGTTGACAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAD_binding_11 NAD_binding_2

Protein Analysis

349

Amino Acids

36.33

Weight (kDa)

9.16

Isoelectric Point (pI)

35.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Shikimate_DH PF01488 48 - 113 9.1e-06 Shikimate / quinate 5-dehydrogenase
NAD_binding_2 PF03446 51 - 209 6.5e-45 NAD binding domain of 6-phosphogluconate dehydrogenase
NAD_binding_11 PF14833 213 - 331 2.7e-33 NAD-binding of NADP-dependent 3-hydroxyisobutyrate dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016018)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G29120
fragaria_vesca FvH4_1g06170
malus_domestica MD02G1065900.v1.1 MD02G1066000.v1.1
prunus_persica Prupe.7G219600_v2.0.a1
pyrus_communis pycom02g05230
rosa_chinensis RchiOBHm_Chr2g0091951
rosa_laevigata RLG00000016265
rosa_multiflora Rmu_sc0009216.1_g000005 Rmu_sc0017125.1_g000007
rosa_roxburghii Rroxscaffold_2G00149590
rosa_rugosa Rorug02G0021800
rosa_samantha Rh2AG067400 Rh2DG066500
rosa_wichuraiana Rw2G005360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 506, 572
AatII GACGTC 2 cut(s) 324, 525
AccB1I GGYRCC 5 cut(s) 167, 275, 386, 629, 974
AccB7I CCANNNNNTGG 1 cut(s) 895
AccBSI CCGCTC 2 cut(s) 80, 360
AccI GTMKAC 1 cut(s) 423
AccII CGCG 4 cut(s) 153, 300, 503, 530
AclWI GGATC 1 cut(s) 170
AcoI YGGCCR 4 cut(s) 99, 196, 450, 774
AcuI CTGAAG 1 cut(s) 836
AcyI GRCGYC 5 cut(s) 276, 321, 387, 414, 522
AdeI CACNNNGTG 1 cut(s) 856
AfeI AGCGCT 1 cut(s) 984
AfiI CCNNNNNNNGG 9 cut(s) 97, 250, 383, 512, 579, 640, 641, 783, 895
AgsI TTSAA 5 cut(s) 235, 684, 754, 880, 1025
AjnI CCWGG 3 cut(s) 447, 696, 782
AleI CACNNNNGTG 1 cut(s) 152
AluBI AGCT 4 cut(s) 586, 658, 806, 934
AluI AGCT 4 cut(s) 586, 658, 806, 934
Alw26I GTCTC 1 cut(s) 513
AlwI GGATC 1 cut(s) 170
Ama87I CYCGRG 1 cut(s) 635
Aor51HI AGCGCT 1 cut(s) 984
ApeKI GCWGC 1 cut(s) 931
AspLEI GCGC 7 cut(s) 278, 302, 389, 505, 530, 985, 1005
AspS9I GGNCC 8 cut(s) 181, 186, 253, 540, 643, 694, 742, 780
AsuC2I CCSGG 6 cut(s) 408, 636, 637, 778, 834, 912
AsuHPI GGTGA 5 cut(s) 21, 67, 217, 502, 971
AsuNHI GCTAGC 1 cut(s) 658
AvaI CYCGRG 1 cut(s) 635
AvaII GGWCC 3 cut(s) 186, 540, 780
BaeGI GKGCMC 2 cut(s) 634, 977
BanI GGYRCC 5 cut(s) 167, 275, 386, 629, 974
BanII GRGCYC 2 cut(s) 129, 444
BbsI GAAGAC 1 cut(s) 319
BbvI GCAGC 1 cut(s) 943
BccI CCATC 6 cut(s) 63, 148, 207, 236, 341, 680
BceAI ACGGC 3 cut(s) 287, 437, 557
BciT130I CCWGG 3 cut(s) 449, 698, 784
BcnI CCSGG 6 cut(s) 408, 636, 637, 778, 834, 912
BcoDI GTCTC 1 cut(s) 513
BfaI CTAG 1 cut(s) 659
BfoI RGCGCY 4 cut(s) 279, 390, 986, 1006
BglI GCCNNNNNGGC 2 cut(s) 411, 725
BglII AGATCT 1 cut(s) 459
BlpI GCTNAGC 1 cut(s) 927
Bme1390I CCNGG 9 cut(s) 408, 449, 636, 637, 698, 778, 784, 834, 912
Bme18I GGWCC 3 cut(s) 186, 540, 780
BmeT110I CYCGRG 1 cut(s) 635
BmgT120I GGNCC 8 cut(s) 181, 186, 253, 540, 643, 694, 742, 780
BmiI GGNNCC 7 cut(s) 169, 277, 388, 541, 631, 781, 976
BmrFI CCNGG 9 cut(s) 408, 449, 636, 637, 698, 778, 784, 834, 912
BmtI GCTAGC 1 cut(s) 662
BpiI GAAGAC 1 cut(s) 319
BpmI CTGGAG 1 cut(s) 1019
Bpu10I CCTNAGC 1 cut(s) 544
Bpu1102I GCTNAGC 1 cut(s) 927
BpuEI CTTGAG 1 cut(s) 932
BpuMI CCSGG 6 cut(s) 408, 636, 637, 778, 834, 912
BsaHI GRCGYC 5 cut(s) 276, 321, 387, 414, 522
BsaJI CCNNGG 7 cut(s) 96, 249, 406, 447, 634, 635, 911
BsaWI WCCGGW 1 cut(s) 170
BsaXI ACNNNNNCTCC 6 cut(s) 21, 50, 51, 80, 365, 395
Bsc4I CCNNNNNNNGG 9 cut(s) 97, 250, 383, 512, 579, 640, 641, 783, 895
Bse118I RCCGGY 3 cut(s) 183, 212, 512
Bse1I ACTGG 1 cut(s) 646
BseBI CCWGG 3 cut(s) 449, 698, 784
BseDI CCNNGG 7 cut(s) 96, 249, 406, 447, 634, 635, 911
BseLI CCNNNNNNNGG 9 cut(s) 97, 250, 383, 512, 579, 640, 641, 783, 895
BseMII CTCAG 1 cut(s) 941
BseNI ACTGG 1 cut(s) 646
BseRI GAGGAG 5 cut(s) 74, 106, 194, 359, 481
BseSI GKGCMC 2 cut(s) 634, 977
BseX3I CGGCCG 1 cut(s) 774
BseXI GCAGC 1 cut(s) 943
BseYI CCCAGC 2 cut(s) 255, 728
Bsh1236I CGCG 4 cut(s) 153, 300, 503, 530
Bsh1285I CGRYCG 3 cut(s) 498, 768, 777
BshNI GGYRCC 5 cut(s) 167, 275, 386, 629, 974
BsiEI CGRYCG 3 cut(s) 498, 768, 777
BsiHKCI CYCGRG 1 cut(s) 635
BslFI GGGAC 1 cut(s) 553
BslI CCNNNNNNNGG 9 cut(s) 97, 250, 383, 512, 579, 640, 641, 783, 895
BsmAI GTCTC 1 cut(s) 513
BsmBI CGTCTC 1 cut(s) 513
BsmFI GGGAC 1 cut(s) 553
BsoBI CYCGRG 1 cut(s) 635
Bsp1286I GDGCHC 4 cut(s) 129, 444, 634, 977
Bsp143I GATC 4 cut(s) 3, 162, 459, 495
Bsp1720I GCTNAGC 1 cut(s) 927
Bsp19I CCATGG 1 cut(s) 96
BspCNI CTCAG 1 cut(s) 940
BspFNI CGCG 4 cut(s) 153, 300, 503, 530
BspLI GGNNCC 7 cut(s) 169, 277, 388, 541, 631, 781, 976
BspOI GCTAGC 1 cut(s) 662
BspPI GGATC 1 cut(s) 170
BspT107I GGYRCC 5 cut(s) 167, 275, 386, 629, 974
BsrBI CCGCTC 2 cut(s) 80, 360
BsrFI RCCGGY 3 cut(s) 183, 212, 512
BsrI ACTGG 1 cut(s) 646
BssAI RCCGGY 3 cut(s) 183, 212, 512
BssECI CCNNGG 7 cut(s) 96, 249, 406, 447, 634, 635, 911
BssMI GATC 4 cut(s) 3, 162, 459, 495
BssNI GRCGYC 5 cut(s) 276, 321, 387, 414, 522
BssT1I CCWWGG 2 cut(s) 96, 249
Bst2UI CCWGG 3 cut(s) 449, 698, 784
Bst4CI ACNGT 1 cut(s) 1032
Bst6I CTCTTC 2 cut(s) 267, 812
BstACI GRCGYC 5 cut(s) 276, 321, 387, 414, 522
BstC8I GCNNGC 4 cut(s) 214, 559, 660, 903
BstDEI CTNAG 3 cut(s) 544, 807, 927
BstDSI CCRYGG 1 cut(s) 96
BstENI CCTNNNNNAGG 1 cut(s) 248
BstFNI CGCG 4 cut(s) 153, 300, 503, 530
BstH2I RGCGCY 4 cut(s) 279, 390, 986, 1006
BstHHI GCGC 7 cut(s) 278, 302, 389, 505, 530, 985, 1005
BstKTI GATC 4 cut(s) 6, 165, 462, 498
BstMAI GTCTC 1 cut(s) 513
BstMBI GATC 4 cut(s) 3, 162, 459, 495
BstMCI CGRYCG 3 cut(s) 498, 768, 777
BstNI CCWGG 3 cut(s) 449, 698, 784
BstSCI CCNGG 9 cut(s) 406, 447, 634, 635, 696, 776, 782, 832, 910
BstSLI GKGCMC 2 cut(s) 634, 977
BstUI CGCG 4 cut(s) 153, 300, 503, 530
BstV1I GCAGC 1 cut(s) 943
BstV2I GAAGAC 1 cut(s) 319
BstX2I RGATCY 1 cut(s) 459
BstXI CCANNNNNNTGG 3 cut(s) 154, 728, 790
BstYI RGATCY 1 cut(s) 459
BstZI CGGCCG 1 cut(s) 774
BtgI CCRYGG 1 cut(s) 96
Cac8I GCNNGC 4 cut(s) 214, 559, 660, 903
CfoI GCGC 7 cut(s) 278, 302, 389, 505, 530, 985, 1005
Cfr10I RCCGGY 3 cut(s) 183, 212, 512
Cfr13I GGNCC 8 cut(s) 181, 186, 253, 540, 643, 694, 742, 780
Cfr9I CCCGGG 1 cut(s) 635
CseI GACGC 2 cut(s) 403, 509
DdeI CTNAG 3 cut(s) 544, 807, 927
DinI GGCGCC 2 cut(s) 277, 388
DpnI GATC 4 cut(s) 5, 164, 461, 497
DpnII GATC 4 cut(s) 3, 162, 459, 495
DraIII CACNNNGTG 1 cut(s) 856
DrdI GACNNNNNNGTC 2 cut(s) 506, 572
DseDI GACNNNNNNGTC 2 cut(s) 506, 572
EaeI YGGCCR 4 cut(s) 99, 196, 450, 774
EagI CGGCCG 1 cut(s) 774
Eam1104I CTCTTC 2 cut(s) 267, 812
EarI CTCTTC 2 cut(s) 267, 812
EciI GGCGGA 4 cut(s) 77, 392, 460, 531
EclXI CGGCCG 1 cut(s) 774
Eco130I CCWWGG 2 cut(s) 96, 249
Eco24I GRGCYC 2 cut(s) 129, 444
Eco47I GGWCC 3 cut(s) 186, 540, 780
Eco47III AGCGCT 1 cut(s) 984
Eco52I CGGCCG 1 cut(s) 774
Eco57I CTGAAG 1 cut(s) 836
Eco88I CYCGRG 1 cut(s) 635
EcoNI CCTNNNNNAGG 1 cut(s) 248
EcoO109I RGGNCCY 1 cut(s) 540
EcoRII CCWGG 3 cut(s) 447, 696, 782
EcoT14I CCWWGG 2 cut(s) 96, 249
EcoT38I GRGCYC 2 cut(s) 129, 444
EgeI GGCGCC 2 cut(s) 277, 388
EheI GGCGCC 2 cut(s) 277, 388
ErhI CCWWGG 2 cut(s) 96, 249
Esp3I CGTCTC 1 cut(s) 513
FaqI GGGAC 1 cut(s) 553
FauI CCCGC 2 cut(s) 158, 550
FauNDI CATATG 1 cut(s) 677
FblI GTMKAC 1 cut(s) 423
FriOI GRGCYC 2 cut(s) 129, 444
FspBI CTAG 1 cut(s) 659
GlaI GCGC 7 cut(s) 277, 301, 388, 504, 529, 984, 1004
GsaI CCCAGC 2 cut(s) 259, 732
GsuI CTGGAG 1 cut(s) 1019
HaeII RGCGCY 4 cut(s) 279, 390, 986, 1006
HgaI GACGC 2 cut(s) 403, 509
HhaI GCGC 7 cut(s) 278, 302, 389, 505, 530, 985, 1005
Hin1I GRCGYC 5 cut(s) 276, 321, 387, 414, 522
Hin6I GCGC 7 cut(s) 276, 300, 387, 503, 528, 983, 1003
HinP1I GCGC 7 cut(s) 276, 300, 387, 503, 528, 983, 1003
HincII GTYRAC 2 cut(s) 424, 1041
HindII GTYRAC 2 cut(s) 424, 1041
HinfI GANTC 7 cut(s) 174, 290, 550, 589, 811, 837, 847
HphI GGTGA 5 cut(s) 21, 67, 217, 502, 971
Hpy166II GTNNAC 2 cut(s) 424, 1041
Hpy188I TCNGA 4 cut(s) 319, 352, 439, 816
Hpy188III TCNNGA 4 cut(s) 266, 457, 751, 998
Hpy8I GTNNAC 2 cut(s) 424, 1041
HpyAV CCTTC 6 cut(s) 50, 634, 698, 748, 853, 874
HpyCH4III ACNGT 1 cut(s) 1032
HpyCH4IV ACGT 2 cut(s) 321, 522
HpyCH4V TGCA 2 cut(s) 653, 799
HpyF3I CTNAG 3 cut(s) 544, 807, 927
HpySE526I ACGT 2 cut(s) 321, 522
Hsp92I GRCGYC 5 cut(s) 276, 321, 387, 414, 522
HspAI GCGC 7 cut(s) 276, 300, 387, 503, 528, 983, 1003
KasI GGCGCC 2 cut(s) 275, 386
KroI GCCGGC 1 cut(s) 212
KroNI GCCGGC 1 cut(s) 214
Kzo9I GATC 4 cut(s) 3, 162, 459, 495
LmnI GCTCC 4 cut(s) 365, 494, 803, 1000
Lsp1109I GCAGC 1 cut(s) 943
MaeI CTAG 1 cut(s) 659
MaeII ACGT 2 cut(s) 321, 522
MaeIII GTNAC 3 cut(s) 508, 942, 1017
MalI GATC 4 cut(s) 5, 164, 461, 497
MbiI CCGCTC 2 cut(s) 80, 360
MboI GATC 4 cut(s) 3, 162, 459, 495
MboII GAAGA 4 cut(s) 223, 254, 319, 829
MflI RGATCY 1 cut(s) 459
MhlI GDGCHC 4 cut(s) 129, 444, 634, 977
MluCI AATT 2 cut(s) 619, 967
Mly113I GGCGCC 2 cut(s) 276, 387
MlyI GAGTC 1 cut(s) 183
MmeI TCCRAC 2 cut(s) 342, 771
MroNI GCCGGC 1 cut(s) 212
MseI TTAA 1 cut(s) 618
MslI CAYNNNNRTG 1 cut(s) 152
MspR9I CCNGG 9 cut(s) 408, 449, 636, 637, 698, 778, 784, 834, 912
MvaI CCWGG 3 cut(s) 449, 698, 784
MvnI CGCG 4 cut(s) 153, 300, 503, 530
NaeI GCCGGC 1 cut(s) 214
NarI GGCGCC 2 cut(s) 276, 387
NciI CCSGG 6 cut(s) 408, 636, 637, 778, 834, 912
NcoI CCATGG 1 cut(s) 96
NdeI CATATG 1 cut(s) 677
NdeII GATC 4 cut(s) 3, 162, 459, 495
NgoMIV GCCGGC 1 cut(s) 212
NheI GCTAGC 1 cut(s) 658
NlaIV GGNNCC 7 cut(s) 169, 277, 388, 541, 631, 781, 976
NmuCI GTSAC 3 cut(s) 508, 942, 1017
OliI CACNNNNGTG 1 cut(s) 152
PcsI WCGNNNNNNNCGW 2 cut(s) 504, 573
PdiI GCCGGC 1 cut(s) 214
PfeI GAWTC 6 cut(s) 290, 550, 589, 811, 837, 847
PflFI GACNNNGTC 1 cut(s) 323
PflMI CCANNNNNTGG 1 cut(s) 895
Ple19I CGATCG 1 cut(s) 498
PleI GAGTC 1 cut(s) 182
PluTI GGCGCC 2 cut(s) 279, 390
PpsI GAGTC 1 cut(s) 182
PpuMI RGGWCCY 1 cut(s) 540
Psp5II RGGWCCY 1 cut(s) 540
Psp6I CCWGG 3 cut(s) 447, 696, 782
PspFI CCCAGC 2 cut(s) 255, 728
PspGI CCWGG 3 cut(s) 447, 696, 782
PspN4I GGNNCC 7 cut(s) 169, 277, 388, 541, 631, 781, 976
PspPI GGNCC 8 cut(s) 181, 186, 253, 540, 643, 694, 742, 780
PspPPI RGGWCCY 1 cut(s) 540
PsuI RGATCY 1 cut(s) 459
PsyI GACNNNGTC 1 cut(s) 323
PvuI CGATCG 1 cut(s) 498
RseI CAYNNNNRTG 1 cut(s) 152
SalI GTCGAC 1 cut(s) 422
SaqAI TTAA 1 cut(s) 618
Sau3AI GATC 4 cut(s) 3, 162, 459, 495
Sau96I GGNCC 8 cut(s) 181, 186, 253, 540, 643, 694, 742, 780
SchI GAGTC 1 cut(s) 183
ScrFI CCNGG 9 cut(s) 408, 449, 636, 637, 698, 778, 784, 834, 912
SduI GDGCHC 4 cut(s) 129, 444, 634, 977
SfoI GGCGCC 2 cut(s) 277, 388
SgrAI CRCCGGYG 1 cut(s) 512
SinI GGWCC 3 cut(s) 186, 540, 780
SmaI CCCGGG 1 cut(s) 637
SmiMI CAYNNNNRTG 1 cut(s) 152
SmlI CTYRAG 1 cut(s) 947
SmoI CTYRAG 1 cut(s) 947
Sse9I AATT 2 cut(s) 619, 967
SspDI GGCGCC 2 cut(s) 275, 386
SspMI CTAG 1 cut(s) 659
StyD4I CCNGG 9 cut(s) 406, 447, 634, 635, 696, 776, 782, 832, 910
StyI CCWWGG 2 cut(s) 96, 249
TaaI ACNGT 1 cut(s) 1032
TaiI ACGT 2 cut(s) 324, 525
TaqI TCGA 7 cut(s) 267, 372, 423, 456, 494, 498, 828
TaqII GACCGA 1 cut(s) 754
TasI AATT 2 cut(s) 619, 967
TfiI GAWTC 6 cut(s) 290, 550, 589, 811, 837, 847
Tru1I TTAA 1 cut(s) 618
Tru9I TTAA 1 cut(s) 618
TseFI GTSAC 3 cut(s) 508, 942, 1017
TseI GCWGC 1 cut(s) 931
Tsp45I GTSAC 3 cut(s) 508, 942, 1017
TspGWI ACGGA 1 cut(s) 352
TspMI CCCGGG 1 cut(s) 635
Tth111I GACNNNGTC 1 cut(s) 323
Van91I CCANNNNNTGG 1 cut(s) 895
VpaK11BI GGWCC 3 cut(s) 186, 540, 780
XagI CCTNNNNNAGG 1 cut(s) 248
XmaI CCCGGG 1 cut(s) 635
XmiI GTMKAC 1 cut(s) 423
XspI CTAG 1 cut(s) 659
ZraI GACGTC 2 cut(s) 322, 523
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.