Rh2AG067400

3-hydroxyisobutyrate dehydrogenase-like 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0016018
Physical Location & Seq
Forward (+)
5595692 .. 5597270
1579 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG067400.1

Sequence Viewer

Length: 1038 bp
ATGATCCTCAAAATAGTTTCACCTCCACTCCTTCCCCAACTCTCCCATCACTTCCACTCCCACTTCACCGCTCTCCTCCGCCGCACCATGGCCGCCGCCTCCTCCACCGAGCCCACTATAAGCCCATCAACCACCCGCGTGGGCTGGATCGGCACCGGCGTCATGGGCCGGTCCATGTGCGGCCACCTCCTCAAAGCCGGCTACACTCTCACCATCTTCAACCGCACCCTATCCAAGGCCCAGCCGCTTCTCGATTTGGGCGCCAAACTCGCCGATTCCCCACGCGCCGTCGCCTCCCAATCCGACGTCGTCTTCTCCATCGTCGGCTACCCCTCCGACGTCCGCTCCGTCCTCCTCGACCCCACCTCCGGCGCTCTCTCCGGCCTCCGCCCCGGCGGCGTCCTCGTCGACATGACCACCTCCGAGCCCTCCCTCGCCGTCGAGATCTCCCAATCCGCCGCCGCCAAATCCTGCTCCTCGATCGACGCGCCCGTCACCGGCGGAGACGTCGGCGCGAAAAACGGGACCTTAGCGATTCTGGCGGGCGGCGACGAAGCCGTCGTGGGGAAGCTGATTCCCCTTTTTGCCCTCATGGGTAAGGTTAATTACATGGGTGCCCCCGGGAAGGGCCAGTTTGCAAAGCTAGCGAACCAGACCATCATATGTTCAACAATGTTGGGCCTAGTAGAAGGCATGGTTTTTGCCCACAAGGCTGGGCTTGATGTGGGCCTGTTCTTGAAGGCAATCTCGGTCGGGGCGGCCGGGTCCAGGTCGTTGGATTTGTATGGAGCTAGGATTCTGAAGAGGGATTTCGAGCCGGGATTCTATGTGAATCACTTTGTGAAGGACTTGGGGATTTGTTTGAAGGAGTGTCAGAACATGGGGCTGGCTCTGCCGGGGTTGGCTCTGGCTCAGCAGCTTTACTTGTCACTCAAGGCTCATGGTGAGGGCAATTTGGGCACCCAGTCGCTCATTGTGGCTCTGGAGCGCCTCAACAATGTGTCACTTGAACAACAGTCTGAACCGTCAAAGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAD_binding_11 NAD_binding_2

Protein Analysis

345

Amino Acids

36.05

Weight (kDa)

9.04

Isoelectric Point (pI)

37.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Shikimate_DH PF01488 43 - 108 8.9e-06 Shikimate / quinate 5-dehydrogenase
NAD_binding_2 PF03446 46 - 204 6.3e-45 NAD binding domain of 6-phosphogluconate dehydrogenase
NAD_binding_11 PF14833 208 - 325 1.9e-33 NAD-binding of NADP-dependent 3-hydroxyisobutyrate dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016018)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G29120
fragaria_vesca FvH4_1g06170
malus_domestica MD02G1065900.v1.1 MD02G1066000.v1.1
prunus_persica Prupe.7G219600_v2.0.a1
pyrus_communis pycom02g05230
rosa_chinensis RchiOBHm_Chr2g0091951
rosa_laevigata RLG00000016265
rosa_multiflora Rmu_sc0009216.1_g000005 Rmu_sc0017125.1_g000007
rosa_roxburghii Rroxscaffold_2G00149590
rosa_rugosa Rorug02G0021800
rosa_samantha Rh2AG067400 Rh2DG066500
rosa_wichuraiana Rw2G005360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 491, 557
AatII GACGTC 3 cut(s) 309, 342, 510
AccB1I GGYRCC 4 cut(s) 152, 260, 614, 959
AccBSI CCGCTC 2 cut(s) 71, 345
AccI GTMKAC 1 cut(s) 408
AccII CGCG 4 cut(s) 138, 285, 488, 515
AclWI GGATC 1 cut(s) 155
AcoI YGGCCR 3 cut(s) 90, 181, 759
AcuI CTGAAG 1 cut(s) 821
AcyI GRCGYC 6 cut(s) 159, 261, 306, 339, 399, 507
AdeI CACNNNGTG 1 cut(s) 841
AfiI CCNNNNNNNGG 8 cut(s) 88, 235, 368, 497, 564, 625, 626, 768
AgsI TTSAA 5 cut(s) 220, 669, 739, 865, 1010
AjnI CCWGG 1 cut(s) 767
AleI CACNNNNGTG 1 cut(s) 137
AluBI AGCT 4 cut(s) 571, 643, 791, 919
AluI AGCT 4 cut(s) 571, 643, 791, 919
Alw26I GTCTC 1 cut(s) 498
AlwI GGATC 1 cut(s) 155
Ama87I CYCGRG 1 cut(s) 620
AoxI GGCC 9 cut(s) 90, 166, 181, 237, 382, 628, 679, 727, 759
ApeKI GCWGC 1 cut(s) 916
AspLEI GCGC 6 cut(s) 263, 287, 374, 490, 515, 990
AspS9I GGNCC 8 cut(s) 166, 171, 238, 525, 628, 679, 727, 765
AsuC2I CCSGG 6 cut(s) 393, 621, 622, 763, 819, 897
AsuHPI GGTGA 5 cut(s) 12, 58, 202, 487, 956
AsuNHI GCTAGC 1 cut(s) 643
AvaI CYCGRG 1 cut(s) 620
AvaII GGWCC 3 cut(s) 171, 525, 765
BaeGI GKGCMC 2 cut(s) 619, 962
BanI GGYRCC 4 cut(s) 152, 260, 614, 959
BanII GRGCYC 2 cut(s) 114, 429
BbsI GAAGAC 1 cut(s) 304
BbvI GCAGC 1 cut(s) 928
BccI CCATC 5 cut(s) 54, 133, 221, 326, 665
BceAI ACGGC 3 cut(s) 272, 422, 542
BciT130I CCWGG 1 cut(s) 769
BcnI CCSGG 6 cut(s) 393, 621, 622, 763, 819, 897
BcoDI GTCTC 1 cut(s) 498
BfaI CTAG 3 cut(s) 644, 683, 792
BfoI RGCGCY 3 cut(s) 264, 375, 991
BglI GCCNNNNNGGC 2 cut(s) 396, 710
BglII AGATCT 1 cut(s) 444
BlpI GCTNAGC 1 cut(s) 912
Bme1390I CCNGG 7 cut(s) 393, 621, 622, 763, 769, 819, 897
Bme18I GGWCC 3 cut(s) 171, 525, 765
BmeT110I CYCGRG 1 cut(s) 620
BmgT120I GGNCC 8 cut(s) 166, 171, 238, 525, 628, 679, 727, 765
BmiI GGNNCC 6 cut(s) 154, 262, 526, 616, 766, 961
BmrFI CCNGG 7 cut(s) 393, 621, 622, 763, 769, 819, 897
BmrI ACTGGG 1 cut(s) 958
BmtI GCTAGC 1 cut(s) 647
BmuI ACTGGG 1 cut(s) 958
BpiI GAAGAC 1 cut(s) 304
BpmI CTGGAG 1 cut(s) 1004
Bpu10I CCTNAGC 1 cut(s) 529
Bpu1102I GCTNAGC 1 cut(s) 912
BpuEI CTTGAG 1 cut(s) 917
BpuMI CCSGG 6 cut(s) 393, 621, 622, 763, 819, 897
BsaHI GRCGYC 6 cut(s) 159, 261, 306, 339, 399, 507
BsaJI CCNNGG 6 cut(s) 87, 234, 391, 619, 620, 896
BsaXI ACNNNNNCTCC 8 cut(s) 12, 41, 42, 71, 329, 350, 359, 380
Bsc4I CCNNNNNNNGG 8 cut(s) 88, 235, 368, 497, 564, 625, 626, 768
Bse118I RCCGGY 4 cut(s) 155, 168, 197, 497
Bse1I ACTGG 2 cut(s) 631, 964
BseBI CCWGG 1 cut(s) 769
BseDI CCNNGG 6 cut(s) 87, 234, 391, 619, 620, 896
BseLI CCNNNNNNNGG 8 cut(s) 88, 235, 368, 497, 564, 625, 626, 768
BseMII CTCAG 1 cut(s) 926
BseNI ACTGG 2 cut(s) 631, 964
BseRI GAGGAG 5 cut(s) 65, 91, 179, 344, 466
BseSI GKGCMC 2 cut(s) 619, 962
BseX3I CGGCCG 1 cut(s) 759
BseXI GCAGC 1 cut(s) 928
BseYI CCCAGC 2 cut(s) 240, 713
Bsh1236I CGCG 4 cut(s) 138, 285, 488, 515
Bsh1285I CGRYCG 3 cut(s) 483, 753, 762
BshFI GGCC 9 cut(s) 92, 168, 183, 239, 384, 630, 681, 729, 761
BshNI GGYRCC 4 cut(s) 152, 260, 614, 959
BsiEI CGRYCG 3 cut(s) 483, 753, 762
BsiHKCI CYCGRG 1 cut(s) 620
BslFI GGGAC 1 cut(s) 538
BslI CCNNNNNNNGG 8 cut(s) 88, 235, 368, 497, 564, 625, 626, 768
BsmAI GTCTC 1 cut(s) 498
BsmBI CGTCTC 1 cut(s) 498
BsmFI GGGAC 1 cut(s) 538
BsnI GGCC 9 cut(s) 92, 168, 183, 239, 384, 630, 681, 729, 761
BsoBI CYCGRG 1 cut(s) 620
Bsp1286I GDGCHC 4 cut(s) 114, 429, 619, 962
Bsp143I GATC 4 cut(s) 3, 147, 444, 480
Bsp1720I GCTNAGC 1 cut(s) 912
Bsp19I CCATGG 1 cut(s) 87
BspANI GGCC 9 cut(s) 92, 168, 183, 239, 384, 630, 681, 729, 761
BspCNI CTCAG 1 cut(s) 925
BspFNI CGCG 4 cut(s) 138, 285, 488, 515
BspLI GGNNCC 6 cut(s) 154, 262, 526, 616, 766, 961
BspOI GCTAGC 1 cut(s) 647
BspPI GGATC 1 cut(s) 155
BspT107I GGYRCC 4 cut(s) 152, 260, 614, 959
BsrBI CCGCTC 2 cut(s) 71, 345
BsrFI RCCGGY 4 cut(s) 155, 168, 197, 497
BsrI ACTGG 2 cut(s) 631, 964
BssAI RCCGGY 4 cut(s) 155, 168, 197, 497
BssECI CCNNGG 6 cut(s) 87, 234, 391, 619, 620, 896
BssMI GATC 4 cut(s) 3, 147, 444, 480
BssNI GRCGYC 6 cut(s) 159, 261, 306, 339, 399, 507
BssT1I CCWWGG 2 cut(s) 87, 234
Bst2UI CCWGG 1 cut(s) 769
Bst4CI ACNGT 2 cut(s) 1017, 1026
Bst6I CTCTTC 1 cut(s) 797
BstACI GRCGYC 6 cut(s) 159, 261, 306, 339, 399, 507
BstC8I GCNNGC 4 cut(s) 199, 544, 645, 888
BstDEI CTNAG 2 cut(s) 529, 912
BstDSI CCRYGG 1 cut(s) 87
BstENI CCTNNNNNAGG 1 cut(s) 233
BstFNI CGCG 4 cut(s) 138, 285, 488, 515
BstH2I RGCGCY 3 cut(s) 264, 375, 991
BstHHI GCGC 6 cut(s) 263, 287, 374, 490, 515, 990
BstKTI GATC 4 cut(s) 6, 150, 447, 483
BstMAI GTCTC 1 cut(s) 498
BstMBI GATC 4 cut(s) 3, 147, 444, 480
BstMCI CGRYCG 3 cut(s) 483, 753, 762
BstMWI GCNNNNNNNGC 9 cut(s) 150, 165, 269, 396, 539, 644, 710, 892, 957
BstNI CCWGG 1 cut(s) 769
BstSCI CCNGG 7 cut(s) 391, 619, 620, 761, 767, 817, 895
BstSLI GKGCMC 2 cut(s) 619, 962
BstUI CGCG 4 cut(s) 138, 285, 488, 515
BstV1I GCAGC 1 cut(s) 928
BstV2I GAAGAC 1 cut(s) 304
BstX2I RGATCY 1 cut(s) 444
BstXI CCANNNNNNTGG 3 cut(s) 139, 713, 775
BstYI RGATCY 1 cut(s) 444
BstZI CGGCCG 1 cut(s) 759
BsuRI GGCC 9 cut(s) 92, 168, 183, 239, 384, 630, 681, 729, 761
BtgI CCRYGG 1 cut(s) 87
Cac8I GCNNGC 4 cut(s) 199, 544, 645, 888
CfoI GCGC 6 cut(s) 263, 287, 374, 490, 515, 990
Cfr10I RCCGGY 4 cut(s) 155, 168, 197, 497
Cfr13I GGNCC 8 cut(s) 166, 171, 238, 525, 628, 679, 727, 765
Cfr9I CCCGGG 1 cut(s) 620
CseI GACGC 3 cut(s) 148, 388, 494
CviAII CATG 9 cut(s) 88, 163, 175, 412, 592, 610, 694, 880, 941
DdeI CTNAG 2 cut(s) 529, 912
DinI GGCGCC 1 cut(s) 262
DpnI GATC 4 cut(s) 5, 149, 446, 482
DpnII GATC 4 cut(s) 3, 147, 444, 480
DraIII CACNNNGTG 1 cut(s) 841
DrdI GACNNNNNNGTC 2 cut(s) 491, 557
DseDI GACNNNNNNGTC 2 cut(s) 491, 557
EaeI YGGCCR 3 cut(s) 90, 181, 759
EagI CGGCCG 1 cut(s) 759
Eam1104I CTCTTC 1 cut(s) 797
EarI CTCTTC 1 cut(s) 797
EciI GGCGGA 4 cut(s) 68, 377, 445, 516
EclXI CGGCCG 1 cut(s) 759
Eco130I CCWWGG 2 cut(s) 87, 234
Eco24I GRGCYC 2 cut(s) 114, 429
Eco47I GGWCC 3 cut(s) 171, 525, 765
Eco52I CGGCCG 1 cut(s) 759
Eco57I CTGAAG 1 cut(s) 821
Eco88I CYCGRG 1 cut(s) 620
EcoNI CCTNNNNNAGG 1 cut(s) 233
EcoO109I RGGNCCY 1 cut(s) 525
EcoRII CCWGG 1 cut(s) 767
EcoT14I CCWWGG 2 cut(s) 87, 234
EcoT38I GRGCYC 2 cut(s) 114, 429
EgeI GGCGCC 1 cut(s) 262
EheI GGCGCC 1 cut(s) 262
ErhI CCWWGG 2 cut(s) 87, 234
Esp3I CGTCTC 1 cut(s) 498
FaeI CATG 9 cut(s) 91, 166, 178, 415, 595, 613, 697, 883, 944
FaqI GGGAC 1 cut(s) 538
FatI CATG 9 cut(s) 87, 162, 174, 411, 591, 609, 693, 879, 940
FauI CCCGC 2 cut(s) 143, 535
FauNDI CATATG 1 cut(s) 662
FblI GTMKAC 1 cut(s) 408
FriOI GRGCYC 2 cut(s) 114, 429
FspBI CTAG 3 cut(s) 644, 683, 792
GlaI GCGC 6 cut(s) 262, 286, 373, 489, 514, 989
GsaI CCCAGC 2 cut(s) 244, 717
GsuI CTGGAG 1 cut(s) 1004
HaeII RGCGCY 3 cut(s) 264, 375, 991
HaeIII GGCC 9 cut(s) 92, 168, 183, 239, 384, 630, 681, 729, 761
HgaI GACGC 3 cut(s) 148, 388, 494
HhaI GCGC 6 cut(s) 263, 287, 374, 490, 515, 990
Hin1I GRCGYC 6 cut(s) 159, 261, 306, 339, 399, 507
Hin1II CATG 9 cut(s) 91, 166, 178, 415, 595, 613, 697, 883, 944
Hin6I GCGC 6 cut(s) 261, 285, 372, 488, 513, 988
HinP1I GCGC 6 cut(s) 261, 285, 372, 488, 513, 988
HincII GTYRAC 1 cut(s) 409
HindII GTYRAC 1 cut(s) 409
HinfI GANTC 6 cut(s) 275, 535, 574, 796, 822, 832
HphI GGTGA 5 cut(s) 12, 58, 202, 487, 956
Hpy166II GTNNAC 1 cut(s) 409
Hpy188I TCNGA 6 cut(s) 304, 337, 424, 801, 876, 1021
Hpy188III TCNNGA 4 cut(s) 251, 442, 736, 983
Hpy8I GTNNAC 1 cut(s) 409
HpyAV CCTTC 6 cut(s) 41, 619, 683, 733, 838, 859
HpyCH4III ACNGT 2 cut(s) 1017, 1026
HpyCH4IV ACGT 3 cut(s) 306, 339, 507
HpyCH4V TGCA 1 cut(s) 638
HpyF10VI GCNNNNNNNGC 9 cut(s) 150, 165, 269, 396, 539, 644, 710, 892, 957
HpyF3I CTNAG 2 cut(s) 529, 912
HpySE526I ACGT 3 cut(s) 306, 339, 507
Hsp92I GRCGYC 6 cut(s) 159, 261, 306, 339, 399, 507
Hsp92II CATG 9 cut(s) 91, 166, 178, 415, 595, 613, 697, 883, 944
HspAI GCGC 6 cut(s) 261, 285, 372, 488, 513, 988
KasI GGCGCC 1 cut(s) 260
KroI GCCGGC 1 cut(s) 197
KroNI GCCGGC 1 cut(s) 199
Kzo9I GATC 4 cut(s) 3, 147, 444, 480
LmnI GCTCC 4 cut(s) 350, 479, 788, 985
Lsp1109I GCAGC 1 cut(s) 928
MaeI CTAG 3 cut(s) 644, 683, 792
MaeII ACGT 3 cut(s) 306, 339, 507
MaeIII GTNAC 3 cut(s) 493, 927, 1002
MalI GATC 4 cut(s) 5, 149, 446, 482
MbiI CCGCTC 2 cut(s) 71, 345
MboI GATC 4 cut(s) 3, 147, 444, 480
MboII GAAGA 3 cut(s) 208, 304, 814
MflI RGATCY 1 cut(s) 444
MhlI GDGCHC 4 cut(s) 114, 429, 619, 962
MluCI AATT 2 cut(s) 604, 952
Mly113I GGCGCC 1 cut(s) 261
MmeI TCCRAC 3 cut(s) 327, 360, 756
MroNI GCCGGC 1 cut(s) 197
MseI TTAA 1 cut(s) 603
MslI CAYNNNNRTG 1 cut(s) 137
MspR9I CCNGG 7 cut(s) 393, 621, 622, 763, 769, 819, 897
MvaI CCWGG 1 cut(s) 769
MvnI CGCG 4 cut(s) 138, 285, 488, 515
MwoI GCNNNNNNNGC 9 cut(s) 150, 165, 269, 396, 539, 644, 710, 892, 957
NaeI GCCGGC 1 cut(s) 199
NarI GGCGCC 1 cut(s) 261
NciI CCSGG 6 cut(s) 393, 621, 622, 763, 819, 897
NcoI CCATGG 1 cut(s) 87
NdeI CATATG 1 cut(s) 662
NdeII GATC 4 cut(s) 3, 147, 444, 480
NgoMIV GCCGGC 1 cut(s) 197
NheI GCTAGC 1 cut(s) 643
NlaIII CATG 9 cut(s) 91, 166, 178, 415, 595, 613, 697, 883, 944
NlaIV GGNNCC 6 cut(s) 154, 262, 526, 616, 766, 961
NmuCI GTSAC 3 cut(s) 493, 927, 1002
OliI CACNNNNGTG 1 cut(s) 137
PcsI WCGNNNNNNNCGW 4 cut(s) 156, 345, 489, 558
PdiI GCCGGC 1 cut(s) 199
PfeI GAWTC 6 cut(s) 275, 535, 574, 796, 822, 832
PflFI GACNNNGTC 1 cut(s) 308
Ple19I CGATCG 1 cut(s) 483
PluTI GGCGCC 1 cut(s) 264
PpuMI RGGWCCY 1 cut(s) 525
Psp5II RGGWCCY 1 cut(s) 525
Psp6I CCWGG 1 cut(s) 767
PspFI CCCAGC 2 cut(s) 240, 713
PspGI CCWGG 1 cut(s) 767
PspN4I GGNNCC 6 cut(s) 154, 262, 526, 616, 766, 961
PspPI GGNCC 8 cut(s) 166, 171, 238, 525, 628, 679, 727, 765
PspPPI RGGWCCY 1 cut(s) 525
PsuI RGATCY 1 cut(s) 444
PsyI GACNNNGTC 1 cut(s) 308
PvuI CGATCG 1 cut(s) 483
RseI CAYNNNNRTG 1 cut(s) 137
SalI GTCGAC 1 cut(s) 407
SaqAI TTAA 1 cut(s) 603
Sau3AI GATC 4 cut(s) 3, 147, 444, 480
Sau96I GGNCC 8 cut(s) 166, 171, 238, 525, 628, 679, 727, 765
ScrFI CCNGG 7 cut(s) 393, 621, 622, 763, 769, 819, 897
SduI GDGCHC 4 cut(s) 114, 429, 619, 962
SfoI GGCGCC 1 cut(s) 262
SgrAI CRCCGGYG 2 cut(s) 155, 497
SinI GGWCC 3 cut(s) 171, 525, 765
SmaI CCCGGG 1 cut(s) 622
SmiMI CAYNNNNRTG 1 cut(s) 137
SmlI CTYRAG 1 cut(s) 932
SmoI CTYRAG 1 cut(s) 932
Sse9I AATT 2 cut(s) 604, 952
SspDI GGCGCC 1 cut(s) 260
SspMI CTAG 3 cut(s) 644, 683, 792
StyD4I CCNGG 7 cut(s) 391, 619, 620, 761, 767, 817, 895
StyI CCWWGG 2 cut(s) 87, 234
TaaI ACNGT 2 cut(s) 1017, 1026
TaiI ACGT 3 cut(s) 309, 342, 510
TaqI TCGA 7 cut(s) 252, 357, 408, 441, 479, 483, 813
TaqII GACCGA 1 cut(s) 739
TasI AATT 2 cut(s) 604, 952
TfiI GAWTC 6 cut(s) 275, 535, 574, 796, 822, 832
Tru1I TTAA 1 cut(s) 603
Tru9I TTAA 1 cut(s) 603
TseFI GTSAC 3 cut(s) 493, 927, 1002
TseI GCWGC 1 cut(s) 916
Tsp45I GTSAC 3 cut(s) 493, 927, 1002
TspGWI ACGGA 1 cut(s) 337
TspMI CCCGGG 1 cut(s) 620
Tth111I GACNNNGTC 1 cut(s) 308
VpaK11BI GGWCC 3 cut(s) 171, 525, 765
XagI CCTNNNNNAGG 1 cut(s) 233
XmaI CCCGGG 1 cut(s) 620
XmiI GTMKAC 1 cut(s) 408
XspI CTAG 3 cut(s) 644, 683, 792
ZraI GACGTC 3 cut(s) 307, 340, 508
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.