pycom02g15810

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0014085
Physical Location & Seq
Reverse (-)
12772779 .. 12776451
3673 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2973 bp
ATGACGCAATTAGCAGCTGTAAAGCCGGTTTTAGTATTATTTTCCGCACTGTTATGCGTTTTAACAGTCGCTGCCTCTTTGGCCGGCGATACTCAGCACTTGATTCAGGTCAAGGCCCAACTGTCCGACCCGGACGGAAATCTCGACGACTGGGTCCCGAGCACGGATCCCAATCCATGCAACTGGATGGGAATTACATGCGACCCGAAGAACCACTCTGTTCTTGCTGTAAACTTATCCGGGTTTAACGTTTCCGGTGGGTTTCCGTTCGGGTTTTGTAGCATTCGGACCCTCCAGAACCTCTCTCTGTCCGTCAACTCCATCAGCGGCTCTCTCCAAACCCACACCCTCTCTCTCTGCTCCCACCTCCATGTTCTCGAGCTCGACAACAACCTCTTCGTCGGATCATTGCCGGAGTTTCAGCCGGAGTTCACCGACTTGCGAATCCTGAACTTTATGGACAACAACTTCACCGGCGACATTCCGGCGAGTTTCGGCCGGTTTCCCGCTTTGCAAGTCCTGATGCTCTCCGGGAACTTACTCACCGGCACAATCCCCAAGTTCTTGGGAAATCTCAGTGAGTTGACTCACTTGGAACTCGCTATCAATCCATTGATGTCCGGCGAGCTACCTTCCGAAATCGGAAACTTGACGAAGCTCCAGAACCTCTTCGCCCCCCAATGCAACCTCAGAGGCCGTATACCGGACTCCATCGGAAACCTGGCGTCCCTTACGAACCTCGATTTGTCCACGAATTCGCTATCCGGTCCGATTCCGGCGAGCATCGGCGGATTGGCAAGCGTGACGCAGATCGAGCTATTCGCAAACCAGATGTTCGGCGAACTGCCCGAGAGCATCGGAAACCTGACTTTTCTCCAGAACTTAGATCTCTCTATGAACGGCTTCACCGGAAAATTCCCAGAAAAAATTGCACGATTGCATTTGGAGTCTCTGAATCTCAACGACAATTTCTTTTCCGGCGAGCTTCCTCCGATCTTGGCTTCCAATCCCAACTTGCACCAGTTCAAAATCTTCAACAACAGCTTCTCCGGCTCCCTCCCGGAAAATCTCGGCCGATATTCCGATTTGGCAGACCTCGACGTCTCGACGAACAAGTTTTCCGGCGAGCTGCCGAAGTTCCTCTGTTACCGGAAAAAGCTCTGGAATTTGATAGTTATGGAGAACCAGTTCTCCGGAAATTTACCTGATACGTTAAACGAGTGTCATTCTCTGGAATACGTTCGAATCGAATTCAATGAGCTCGACGGCGTCGTTTCGGGCAAGTTTTGGGGTCTGCCCGGGCTATATTTCCTGACAATGAATAACAACAGATTCAACGGCAGCGTCTCTCCGTCCATTTCGGCGGCTACGAGATTCTCGACGCTTACGATCTCCAGCAATGAATTTTCCGGCGCGCTTCCGTCGGCGATTTGTAAGCTTACGAACCTCTCGACCCTCGACGTGAGCGACAACCAATTCTCCGGTGACTTGCCGTCGTGCATGACGGAGCTGAATATTTTGCAGAAGTTAAAAATGGAGGTGAACATGTTCTCCGGTCAGATTCCGAGTTCAGTGAGTTCGTGGACCCAGTTGACGGAGCTGAACCTATCTCACAATCACTTATCGGGTCGGATCCCACCGCAACTCGGCTACTTGCCGGTATTGAATTACTTGGATCTCTCGGCAAATTTACTCACCGGTGAGCTTCCGGCCGAGTTGACCAAGCTCAGGCTCAATCAGTTCAACGTCTCGAATAATAAACTGTATGGAAAAATACCTACCGGTTTCGATTACAGTCCCTTCAGCTCGGGTCTACTGGGTAACCCGAACTTGTGCAGTCCGGATCTGGATCACTTCCCAACCTGCTCCAAACCCAAATCTCCTACCCCAATTTTGATAGTCATTTTATCCGTGTGCGTTGTGCTGGTCGTCGGGTCGGTTCTTTCGTTTTTGAAATATCGATCCAAAGCACTTGGCGGTAAAACGAAGCGGCTCTACAGGCTCACCACATTCCAACGGGTCGGGTTTAACGAAGAGGAGGTGATGCAATCATTAACGGAGCAGAATCTGATTGCAACGGGTGGGTCGGGTCATGTGTACAAAGTGCAACTGAAGACGGGGCAGACCGTGGCGGTTAAGAAGCTATGGGGCGGGGCAAGAAAACCCGAAACTGAATCCGTTTTTAGCTCCGAGGTGGAGACTTTGGGTCGGATCCGACATGGCAATATTGTAAAATTGGTGTTTTGTTGTAGTGGTGAGGAAAGTAGAATTTTGGGTTATGAGTACATGGAGAATGGAAGCTTGGGTGATTGTTTGCATGGAGAAAAGGTTGGGCCTTTGGAGGATTGGGCCAAAAGATTCAACATCGCCGTCGGGTCGGCCTACGGCTTGGCCTACCTCCACCACGACTGTGCTCCGGCCATCGTTCACCGGGACGTGAAGAGTAATAACATACTGTTGGACGAGGACTGGACCCCACGGGTGGCGGATTTCGGACTCGCCAAGACTTTGCAGCGTGACATGGCGGCCGGATGTGGTACCATGTCACAGATTGCTGGGTCCTACGGTTATATCGCTCCGGAGTACGCATACACTCTTAAAGTCACTGAGAAGAGTGATGTCTACAGCTTCGGTGTGGTGCTGTTGGAACTGATAACCGGCAAGAGTCCGAATGACGCGTCCTTTGGTGAGAACACAGACATTGTCAAGTGGGTTAGAGAGGCTGCCATGTCTACTCCTGAAGGAGAAGAAGAAGGCAGCAATGACGGCGGCTTCTGCGGCGCCAATCTCTGCCAGATAATCGATCCAAGGGTTGATCCGTCGACATGTAATTACGAAGAGATTGAGAAGGTTTTGAAGGTGGCTCTTCTTTGTACCTCTGCATTCCCAATCAACAGGCCCTCCATGAGAAGGGTGGTTGAATTGCTTAGAGATCAGAAACCATCTCGACCGTCCAAAATGATAAGCCTTTGA

Protein Analysis

991

Amino Acids

108.16

Weight (kDa)

5.42

Isoelectric Point (pI)

33.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 28 - 68 3.1e-11 Leucine rich repeat N-terminal domain
LRR_8 PF13855 147 - 205 1e-06 Leucine rich repeat
LRR_14 PF23598 163 - 274 6.4e-11 Leucine-rich repeat region
LRR_14 PF23598 471 - 560 2.7e-06 Leucine-rich repeat region
Pkinase PF00069 688 - 898 6.9e-40 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 688 - 908 2.2e-37 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014085)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G65710
fragaria_vesca FvH4_1g18670
malus_domestica MD02G1194100.v1.1 MD05G1283300.v1.1 MD15G1304600.v1.1
prunus_persica Prupe.6G212600_v2.0.a1
pyrus_communis pycom02g15810 pycom15g26710
rosa_chinensis RchiOBHm_Chr2g0109521
rosa_laevigata RLG00000017742
rosa_multiflora Rmu_sc0000332.1_g000032
rosa_roxburghii Rroxscaffold_2G00134180
rosa_rugosa Rorug02G0160600
rosa_samantha Rh2AG212900 Rh2BG223100 Rh2CG214700 Rh2DG218500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 152, 1100
AatII GACGTC 1 cut(s) 1104
Acc36I ACCTGC 1 cut(s) 1871
Acc65I GGTACC 1 cut(s) 2537
AccB1I GGYRCC 2 cut(s) 2537, 2780
AccI GTMKAC 5 cut(s) 700, 1813, 2622, 2732, 2822
AccII CGCG 2 cut(s) 1415, 2678
AccIII TCCGGA 3 cut(s) 1193, 1840, 2578
AclI AACGTT 1 cut(s) 249
AcoI YGGCCR 6 cut(s) 81, 496, 1072, 1710, 2418, 2526
AcsI RAATTY 8 cut(s) 754, 914, 1165, 1198, 1250, 1403, 1687, 2268
AcuI CTGAAG 3 cut(s) 1786, 2132, 2760
AcyI GRCGYC 4 cut(s) 725, 1101, 1269, 2781
AfaI GTAC 5 cut(s) 2099, 2285, 2539, 2585, 2875
AfiI CCNNNNNNNGG 7 cut(s) 163, 703, 1594, 1646, 1728, 2482, 2910
AflIII ACRYGT 3 cut(s) 1545, 2676, 2825
AgeI ACCGGT 2 cut(s) 1697, 1781
AhdI GACNNNNNGTC 2 cut(s) 1492, 2205
AjiI CACGTC 2 cut(s) 1462, 2437
AjnI CCWGG 1 cut(s) 720
AjuI GAANNNNNNNTTGG 2 cut(s) 2285, 2317
AleI CACNNNNGTG 1 cut(s) 2409
AloI GAACNNNNNNTCC 8 cut(s) 1103, 1135, 1172, 1175, 1204, 1207, 1535, 1567
Alw21I GWGCWC 4 cut(s) 164, 384, 1263, 2416
Alw26I GTCTC 5 cut(s) 954, 1108, 1351, 1753, 2192
AlwNI CAGNNNCTG 2 cut(s) 71, 2068
Ama87I CYCGRG 5 cut(s) 157, 377, 848, 1298, 1807
Aor13HI TCCGGA 3 cut(s) 1193, 1840, 2578
ApeKI GCWGC 7 cut(s) 14, 71, 1129, 1341, 2512, 2723, 2757
ApoI RAATTY 8 cut(s) 754, 914, 1165, 1198, 1250, 1403, 1687, 2268
ArsI GACNNNNNNTTYG 4 cut(s) 2663, 2666, 2695, 2698
AsiGI ACCGGT 2 cut(s) 1697, 1781
Asp700I GAANNNNTTC 5 cut(s) 668, 902, 1187, 1239, 1547
Asp718I GGTACC 1 cut(s) 2537
AspLEI GCGC 3 cut(s) 1415, 1417, 2783
AsuC2I CCSGG 7 cut(s) 131, 241, 532, 1061, 1299, 1300, 2432
AsuII TTCGAA 1 cut(s) 1243
AvaI CYCGRG 5 cut(s) 157, 377, 848, 1298, 1807
AvaII GGWCC 6 cut(s) 154, 288, 767, 1584, 2472, 2559
BamHI GGATCC 3 cut(s) 166, 1632, 2211
BanI GGYRCC 2 cut(s) 2537, 2780
BanII GRGCYC 2 cut(s) 384, 1263
BarI GAAGNNNNNNTAC 2 cut(s) 1979, 2011
BbsI GAAGAC 1 cut(s) 2120
Bbv12I GWGCWC 4 cut(s) 164, 384, 1263, 2416
BbvI GCAGC 7 cut(s) 26, 58, 1116, 1353, 2524, 2710, 2769
BccI CCATC 5 cut(s) 181, 329, 719, 2429, 2950
BceAI ACGGC 8 cut(s) 681, 916, 1282, 1354, 1477, 2354, 2401, 2782
BciT130I CCWGG 1 cut(s) 722
BcnI CCSGG 7 cut(s) 131, 241, 532, 1061, 1299, 1300, 2432
BcoDI GTCTC 5 cut(s) 954, 1108, 1351, 1753, 2192
BfmI CTRYAG 2 cut(s) 1996, 2623
BfoI RGCGCY 1 cut(s) 2784
BfuAI ACCTGC 1 cut(s) 1871
BglI GCCNNNNNGGC 1 cut(s) 80
BglII AGATCT 1 cut(s) 886
Bme1390I CCNGG 8 cut(s) 131, 241, 532, 722, 1061, 1299, 1300, 2432
Bme18I GGWCC 6 cut(s) 154, 288, 767, 1584, 2472, 2559
BmeRI GACNNNNNGTC 2 cut(s) 1492, 2205
BmeT110I CYCGRG 5 cut(s) 157, 377, 848, 1298, 1807
BmgBI CACGTC 2 cut(s) 1462, 2437
BmrFI CCNGG 8 cut(s) 131, 241, 532, 722, 1061, 1299, 1300, 2432
BmrI ACTGGG 3 cut(s) 160, 1582, 1826
BmsI GCATC 4 cut(s) 513, 792, 864, 2034
BmuI ACTGGG 3 cut(s) 160, 1582, 1826
BpiI GAAGAC 1 cut(s) 2120
BpmI CTGGAG 4 cut(s) 278, 644, 860, 1378
Bpu10I CCTNAGC 1 cut(s) 1727
Bpu14I TTCGAA 1 cut(s) 1243
BpuMI CCSGG 7 cut(s) 131, 241, 532, 1061, 1299, 1300, 2432
Bsa29I ATCGAT 2 cut(s) 1960, 2802
BsaBI GATNNNNATC 2 cut(s) 171, 1848
BsaHI GRCGYC 4 cut(s) 725, 1101, 1269, 2781
BsaJI CCNNGG 5 cut(s) 1298, 2127, 2190, 2477, 2807
Bsc4I CCNNNNNNNGG 7 cut(s) 163, 703, 1594, 1646, 1728, 2482, 2910
Bse118I RCCGGY 9 cut(s) 25, 83, 473, 498, 545, 1657, 1697, 1781, 2657
Bse1I ACTGG 7 cut(s) 155, 188, 1021, 1186, 1588, 1821, 2474
Bse3DI GCAATG 3 cut(s) 407, 1405, 2767
Bse8I GATNNNNATC 2 cut(s) 171, 1848
BseAI TCCGGA 3 cut(s) 1193, 1840, 2578
BseBI CCWGG 1 cut(s) 722
BseCI ATCGAT 2 cut(s) 1960, 2802
BseDI CCNNGG 5 cut(s) 1298, 2127, 2190, 2477, 2807
BseGI GGATG 2 cut(s) 192, 2537
BseJI GATNNNNATC 2 cut(s) 171, 1848
BseLI CCNNNNNNNGG 7 cut(s) 163, 703, 1594, 1646, 1728, 2482, 2910
BseMI GCAATG 3 cut(s) 407, 1405, 2767
BseMII CTCAG 5 cut(s) 107, 589, 703, 1741, 2598
BseNI ACTGG 7 cut(s) 155, 188, 1021, 1186, 1588, 1821, 2474
BsePI GCGCGC 1 cut(s) 1413
BseRI GAGGAG 1 cut(s) 2051
BseX3I CGGCCG 4 cut(s) 496, 1072, 1710, 2526
BseXI GCAGC 7 cut(s) 26, 58, 1116, 1353, 2524, 2710, 2769
BseYI CCCAGC 1 cut(s) 2555
BsgI GTGCAG 1 cut(s) 1855
Bsh1236I CGCG 2 cut(s) 1415, 2678
Bsh1285I CGRYCG 5 cut(s) 499, 1075, 1713, 2529, 2951
BshNI GGYRCC 2 cut(s) 2537, 2780
BshTI ACCGGT 2 cut(s) 1697, 1781
BshVI ATCGAT 2 cut(s) 1960, 2802
BsiEI CGRYCG 5 cut(s) 499, 1075, 1713, 2529, 2951
BsiHKAI GWGCWC 4 cut(s) 164, 384, 1263, 2416
BsiHKCI CYCGRG 5 cut(s) 157, 377, 848, 1298, 1807
BslFI GGGAC 4 cut(s) 140, 712, 1782, 2447
BslI CCNNNNNNNGG 7 cut(s) 163, 703, 1594, 1646, 1728, 2482, 2910
BsmAI GTCTC 5 cut(s) 954, 1108, 1351, 1753, 2192
BsmBI CGTCTC 3 cut(s) 1108, 1351, 1753
BsmFI GGGAC 4 cut(s) 140, 712, 1782, 2447
BsmI GAATGC 2 cut(s) 282, 2882
BsoBI CYCGRG 5 cut(s) 157, 377, 848, 1298, 1807
Bsp119I TTCGAA 1 cut(s) 1243
Bsp1286I GDGCHC 4 cut(s) 164, 384, 1263, 2416
Bsp13I TCCGGA 3 cut(s) 1193, 1840, 2578
Bsp1407I TGTACA 1 cut(s) 2097
BspCNI CTCAG 5 cut(s) 106, 588, 702, 1740, 2599
BspDI ATCGAT 2 cut(s) 1960, 2802
BspEI TCCGGA 3 cut(s) 1193, 1840, 2578
BspFNI CGCG 2 cut(s) 1415, 2678
BspMI ACCTGC 1 cut(s) 1871
BspQI GCTCTTC 1 cut(s) 2871
BspT104I TTCGAA 1 cut(s) 1243
BspT107I GGYRCC 2 cut(s) 2537, 2780
BsrDI GCAATG 3 cut(s) 407, 1405, 2767
BsrFI RCCGGY 9 cut(s) 25, 83, 473, 498, 545, 1657, 1697, 1781, 2657
BsrGI TGTACA 1 cut(s) 2097
BsrI ACTGG 7 cut(s) 155, 188, 1021, 1186, 1588, 1821, 2474
BssAI RCCGGY 9 cut(s) 25, 83, 473, 498, 545, 1657, 1697, 1781, 2657
BssECI CCNNGG 5 cut(s) 1298, 2127, 2190, 2477, 2807
BssHII GCGCGC 1 cut(s) 1413
BssNAI GTATAC 1 cut(s) 701
BssNI GRCGYC 4 cut(s) 725, 1101, 1269, 2781
BssT1I CCWWGG 1 cut(s) 2807
Bst1107I GTATAC 1 cut(s) 701
Bst2UI CCWGG 1 cut(s) 722
Bst6I CTCTTC 7 cut(s) 401, 674, 2028, 2435, 2606, 2832, 2871
BstACI GRCGYC 4 cut(s) 725, 1101, 1269, 2781
BstAUI TGTACA 1 cut(s) 2097
BstBI TTCGAA 1 cut(s) 1243
BstC8I GCNNGC 7 cut(s) 85, 626, 781, 799, 983, 1127, 1415
BstDEI CTNAG 7 cut(s) 93, 575, 689, 883, 1727, 2607, 2927
BstDSI CCRYGG 2 cut(s) 2127, 2477
BstEII GGTNACC 1 cut(s) 1820
BstF5I GGATG 2 cut(s) 192, 2537
BstFNI CGCG 2 cut(s) 1415, 2678
BstH2I RGCGCY 1 cut(s) 2784
BstHHI GCGC 3 cut(s) 1415, 1417, 2783
BstMAI GTCTC 5 cut(s) 954, 1108, 1351, 1753, 2192
BstMCI CGRYCG 5 cut(s) 499, 1075, 1713, 2529, 2951
BstMWI GCNNNNNNNGC 7 cut(s) 80, 814, 1050, 1999, 2766, 2775, 2778
BstNI CCWGG 1 cut(s) 722
BstNSI RCATGY 3 cut(s) 201, 1549, 2829
BstPI GGTNACC 1 cut(s) 1820
BstSCI CCNGG 8 cut(s) 129, 239, 530, 720, 1059, 1297, 1298, 2430
BstSFI CTRYAG 2 cut(s) 1996, 2623
BstUI CGCG 2 cut(s) 1415, 2678
BstV1I GCAGC 7 cut(s) 26, 58, 1116, 1353, 2524, 2710, 2769
BstV2I GAAGAC 1 cut(s) 2120
BstX2I RGATCY 6 cut(s) 166, 886, 1632, 1675, 1843, 2211
BstXI CCANNNNNNTGG 2 cut(s) 183, 565
BstYI RGATCY 6 cut(s) 166, 886, 1632, 1675, 1843, 2211
BstZ17I GTATAC 1 cut(s) 701
BstZI CGGCCG 4 cut(s) 496, 1072, 1710, 2526
Bsu15I ATCGAT 2 cut(s) 1960, 2802
BsuTUI ATCGAT 2 cut(s) 1960, 2802
BtgI CCRYGG 2 cut(s) 2127, 2477
BtgZI GCGATG 1 cut(s) 2350
BtrI CACGTC 2 cut(s) 1462, 2437
BtsCI GGATG 2 cut(s) 192, 2537
BtsIMutI CAGTG 4 cut(s) 47, 583, 1578, 2604
BveI ACCTGC 1 cut(s) 1871
Cac8I GCNNGC 7 cut(s) 85, 626, 781, 799, 983, 1127, 1415
CaiI CAGNNNCTG 2 cut(s) 71, 2068
CfoI GCGC 3 cut(s) 1415, 1417, 2783
Cfr10I RCCGGY 9 cut(s) 25, 83, 473, 498, 545, 1657, 1697, 1781, 2657
Cfr9I CCCGGG 1 cut(s) 1298
ClaI ATCGAT 2 cut(s) 1960, 2802
CpoI CGGWCCG 1 cut(s) 767
CseI GACGC 8 cut(s) 13, 714, 814, 1258, 1333, 1390, 2667, 2684
Csp6I GTAC 5 cut(s) 2098, 2284, 2538, 2584, 2874
CspAI ACCGGT 2 cut(s) 1697, 1781
CspI CGGWCCG 1 cut(s) 767
CviQI GTAC 5 cut(s) 2098, 2284, 2538, 2584, 2874
DdeI CTNAG 7 cut(s) 93, 575, 689, 883, 1727, 2607, 2927
DinI GGCGCC 1 cut(s) 2782
DrdI GACNNNNNNGTC 2 cut(s) 152, 1100
DriI GACNNNNNGTC 2 cut(s) 1492, 2205
DseDI GACNNNNNNGTC 2 cut(s) 152, 1100
EaeI YGGCCR 6 cut(s) 81, 496, 1072, 1710, 2418, 2526
EagI CGGCCG 4 cut(s) 496, 1072, 1710, 2526
Eam1104I CTCTTC 7 cut(s) 401, 674, 2028, 2435, 2606, 2832, 2871
Eam1105I GACNNNNNGTC 2 cut(s) 1492, 2205
EarI CTCTTC 7 cut(s) 401, 674, 2028, 2435, 2606, 2832, 2871
EciI GGCGGA 2 cut(s) 804, 2501
Ecl136II GAGCTC 2 cut(s) 382, 1261
EclXI CGGCCG 4 cut(s) 496, 1072, 1710, 2526
Eco130I CCWWGG 1 cut(s) 2807
Eco24I GRGCYC 2 cut(s) 384, 1263
Eco47I GGWCC 6 cut(s) 154, 288, 767, 1584, 2472, 2559
Eco52I CGGCCG 4 cut(s) 496, 1072, 1710, 2526
Eco53kI GAGCTC 2 cut(s) 382, 1261
Eco57I CTGAAG 3 cut(s) 1786, 2132, 2760
Eco88I CYCGRG 5 cut(s) 157, 377, 848, 1298, 1807
Eco91I GGTNACC 1 cut(s) 1820
EcoICRI GAGCTC 2 cut(s) 382, 1261
EcoO109I RGGNCCY 3 cut(s) 154, 2559, 2898
EcoO65I GGTNACC 1 cut(s) 1820
EcoRI GAATTC 2 cut(s) 754, 1250
EcoRII CCWGG 1 cut(s) 720
EcoT14I CCWWGG 1 cut(s) 2807
EcoT38I GRGCYC 2 cut(s) 384, 1263
EgeI GGCGCC 1 cut(s) 2782
EheI GGCGCC 1 cut(s) 2782
ErhI CCWWGG 1 cut(s) 2807
Esp3I CGTCTC 3 cut(s) 1108, 1351, 1753
FaqI GGGAC 4 cut(s) 140, 712, 1782, 2447
FauI CCCGC 2 cut(s) 514, 2144
FblI GTMKAC 5 cut(s) 700, 1813, 2622, 2732, 2822
FokI GGATG 2 cut(s) 199, 2544
FriOI GRGCYC 2 cut(s) 384, 1263
GlaI GCGC 3 cut(s) 1414, 1416, 2782
GsaI CCCAGC 1 cut(s) 2559
GsuI CTGGAG 4 cut(s) 278, 644, 860, 1378
HaeII RGCGCY 1 cut(s) 2784
HgaI GACGC 8 cut(s) 13, 714, 814, 1258, 1333, 1390, 2667, 2684
HhaI GCGC 3 cut(s) 1415, 1417, 2783
Hin1I GRCGYC 4 cut(s) 725, 1101, 1269, 2781
Hin6I GCGC 3 cut(s) 1413, 1415, 2781
HinP1I GCGC 3 cut(s) 1413, 1415, 2781
HincII GTYRAC 5 cut(s) 316, 585, 1593, 1719, 2823
HindII GTYRAC 5 cut(s) 316, 585, 1593, 1719, 2823
HindIII AAGCTT 2 cut(s) 1436, 2299
HpyAV CCTTC 7 cut(s) 642, 1810, 2735, 2747, 2842, 2851, 2904
HpyCH4IV ACGT 7 cut(s) 249, 1101, 1211, 1239, 1461, 1746, 2436
HpyF10VI GCNNNNNNNGC 7 cut(s) 80, 814, 1050, 1999, 2766, 2775, 2778
HpyF3I CTNAG 7 cut(s) 93, 575, 689, 883, 1727, 2607, 2927
HpySE526I ACGT 7 cut(s) 249, 1101, 1211, 1239, 1461, 1746, 2436
Hsp92I GRCGYC 4 cut(s) 725, 1101, 1269, 2781
HspAI GCGC 3 cut(s) 1413, 1415, 2781
KasI GGCGCC 1 cut(s) 2780
KflI GGGWCCC 1 cut(s) 154
Kpn2I TCCGGA 3 cut(s) 1193, 1840, 2578
KpnI GGTACC 1 cut(s) 2541
KroI GCCGGC 1 cut(s) 83
KroNI GCCGGC 1 cut(s) 85
LguI GCTCTTC 1 cut(s) 2871
Lsp1109I GCAGC 7 cut(s) 26, 58, 1116, 1353, 2524, 2710, 2769
LweI GCATC 4 cut(s) 513, 792, 864, 2034
MaeII ACGT 7 cut(s) 249, 1101, 1211, 1239, 1461, 1746, 2436
MaeIII GTNAC 7 cut(s) 802, 1145, 1484, 1820, 2516, 2544, 2602
MflI RGATCY 6 cut(s) 166, 886, 1632, 1675, 1843, 2211
MhlI GDGCHC 4 cut(s) 164, 384, 1263, 2416
MluI ACGCGT 1 cut(s) 2676
Mly113I GGCGCC 1 cut(s) 2781
MlyI GAGTC 5 cut(s) 580, 701, 956, 2490, 2674
MmeI TCCRAC 8 cut(s) 150, 382, 1610, 2038, 2189, 2239, 2439, 2625
MroI TCCGGA 3 cut(s) 1193, 1840, 2578
MroNI GCCGGC 1 cut(s) 83
MroXI GAANNNNTTC 5 cut(s) 668, 902, 1187, 1239, 1547
MseI TTAA 8 cut(s) 62, 246, 1214, 1529, 2028, 2054, 2136, 2598
MslI CAYNNNNRTG 3 cut(s) 52, 369, 2409
MspA1I CMGCKG 2 cut(s) 17, 327
MspR9I CCNGG 8 cut(s) 131, 241, 532, 722, 1061, 1299, 1300, 2432
Mva1269I GAATGC 2 cut(s) 282, 2882
MvaI CCWGG 1 cut(s) 722
MvnI CGCG 2 cut(s) 1415, 2678
MwoI GCNNNNNNNGC 7 cut(s) 80, 814, 1050, 1999, 2766, 2775, 2778
NaeI GCCGGC 1 cut(s) 85
NarI GGCGCC 1 cut(s) 2781
NciI CCSGG 7 cut(s) 131, 241, 532, 1061, 1299, 1300, 2432
NgoMIV GCCGGC 1 cut(s) 83
NmeAIII GCCGAG 4 cut(s) 1050, 1626, 1661, 1738
NmuCI GTSAC 5 cut(s) 802, 1484, 2516, 2544, 2602
NspI RCATGY 3 cut(s) 201, 1549, 2829
NspV TTCGAA 1 cut(s) 1243
OliI CACNNNNGTG 1 cut(s) 2409
PaeR7I CTCGAG 1 cut(s) 377
PauI GCGCGC 1 cut(s) 1413
PciI ACATGT 2 cut(s) 1545, 2825
PciSI GCTCTTC 1 cut(s) 2871
PctI GAATGC 2 cut(s) 282, 2882
PdiI GCCGGC 1 cut(s) 85
PdmI GAANNNNTTC 5 cut(s) 668, 902, 1187, 1239, 1547
PflFI GACNNNGTC 3 cut(s) 152, 1268, 2702
PfoI TCCNGGA 2 cut(s) 530, 1059
PinAI ACCGGT 2 cut(s) 1697, 1781
PleI GAGTC 5 cut(s) 580, 701, 955, 2490, 2673
PluTI GGCGCC 1 cut(s) 2784
PpsI GAGTC 5 cut(s) 580, 701, 955, 2490, 2673
PpuMI RGGWCCY 2 cut(s) 154, 2559
PscI ACATGT 2 cut(s) 1545, 2825
Psp124BI GAGCTC 2 cut(s) 384, 1263
Psp1406I AACGTT 1 cut(s) 249
Psp5II RGGWCCY 2 cut(s) 154, 2559
Psp6I CCWGG 1 cut(s) 720
PspEI GGTNACC 1 cut(s) 1820
PspFI CCCAGC 1 cut(s) 2555
PspGI CCWGG 1 cut(s) 720
PspPPI RGGWCCY 2 cut(s) 154, 2559
PstNI CAGNNNCTG 2 cut(s) 71, 2068
PsuI RGATCY 6 cut(s) 166, 886, 1632, 1675, 1843, 2211
PsyI GACNNNGTC 3 cut(s) 152, 1268, 2702
PteI GCGCGC 1 cut(s) 1413
PvuII CAGCTG 1 cut(s) 17
RsaI GTAC 5 cut(s) 2099, 2285, 2539, 2585, 2875
RsaNI GTAC 5 cut(s) 2098, 2284, 2538, 2584, 2874
RseI CAYNNNNRTG 3 cut(s) 52, 369, 2409
Rsr2I CGGWCCG 1 cut(s) 767
RsrII CGGWCCG 1 cut(s) 767
SacI GAGCTC 2 cut(s) 384, 1263
SalI GTCGAC 1 cut(s) 2821
SapI GCTCTTC 1 cut(s) 2871
SaqAI TTAA 8 cut(s) 62, 246, 1214, 1529, 2028, 2054, 2136, 2598
SchI GAGTC 5 cut(s) 580, 701, 956, 2490, 2674
ScrFI CCNGG 8 cut(s) 131, 241, 532, 722, 1061, 1299, 1300, 2432
SduI GDGCHC 4 cut(s) 164, 384, 1263, 2416
SfaNI GCATC 4 cut(s) 513, 792, 864, 2034
SfcI CTRYAG 2 cut(s) 1996, 2623
SfoI GGCGCC 1 cut(s) 2782
Sfr274I CTCGAG 1 cut(s) 377
SfuI TTCGAA 1 cut(s) 1243
SgrAI CRCCGGYG 2 cut(s) 473, 1697
SinI GGWCC 6 cut(s) 154, 288, 767, 1584, 2472, 2559
SlaI CTCGAG 1 cut(s) 377
SmaI CCCGGG 1 cut(s) 1300
SmiMI CAYNNNNRTG 3 cut(s) 52, 369, 2409
SmlI CTYRAG 1 cut(s) 377
SmoI CTYRAG 1 cut(s) 377
SrfI GCCCGGGC 1 cut(s) 1300
SspDI GGCGCC 1 cut(s) 2780
SspI AATATT 2 cut(s) 1516, 2227
SstI GAGCTC 2 cut(s) 384, 1263
StyD4I CCNGG 8 cut(s) 129, 239, 530, 720, 1059, 1297, 1298, 2430
StyI CCWWGG 1 cut(s) 2807
TaiI ACGT 7 cut(s) 252, 1104, 1214, 1242, 1464, 1749, 2439
TatI WGTACW 2 cut(s) 2097, 2283
TauI GCSGC 6 cut(s) 330, 1367, 1993, 2528, 2772, 2781
Tru1I TTAA 8 cut(s) 62, 246, 1214, 1529, 2028, 2054, 2136, 2598
Tru9I TTAA 8 cut(s) 62, 246, 1214, 1529, 2028, 2054, 2136, 2598
TscAI CASTG 4 cut(s) 54, 583, 1578, 2611
TseFI GTSAC 5 cut(s) 802, 1484, 2516, 2544, 2602
TseI GCWGC 7 cut(s) 14, 71, 1129, 1341, 2512, 2723, 2757
Tsp45I GTSAC 5 cut(s) 802, 1484, 2516, 2544, 2602
TspDTI ATGAA 3 cut(s) 911, 1334, 1416
TspMI CCCGGG 1 cut(s) 1298
TspRI CASTG 4 cut(s) 54, 583, 1578, 2611
Tth111I GACNNNGTC 3 cut(s) 152, 1268, 2702
VpaK11BI GGWCC 6 cut(s) 154, 288, 767, 1584, 2472, 2559
XapI RAATTY 8 cut(s) 754, 914, 1165, 1198, 1250, 1403, 1687, 2268
XceI RCATGY 3 cut(s) 201, 1549, 2829
XcmI CCANNNNNNNNNTGG 2 cut(s) 718, 2911
XhoI CTCGAG 1 cut(s) 377
XmaI CCCGGG 1 cut(s) 1298
XmiI GTMKAC 5 cut(s) 700, 1813, 2622, 2732, 2822
XmnI GAANNNNTTC 5 cut(s) 668, 902, 1187, 1239, 1547
ZraI GACGTC 1 cut(s) 1102
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.