pycom02g23060

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0007527
Physical Location & Seq
Reverse (-)
21178475 .. 21182365
3891 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1488 bp
ATGCTCCAACTACATACAACTCGTTCTTGGGATTTCTTGGAGACGGAATTGGCCAGGCCATTCAAGAACAAATATCTGCACCCATCAAGTGATGTTATAATTGGGATGATAGACACAGGGATATGGCCTGAGTCTCCGAGCTTCAGTGATGAGGGAATTGGTGCAGTCCCTTCAAGATGGAAAGGAGTCTGTATGGAAGGCTTGGACTTCAAGAAATCTAATTGTAATAGAAAGTTGATAGGTGCAAGATACTATAATGTTCCGGTGACAACAAATGGTGACCAGAGCCATTTAGCCAGTACCAGTGGCTCACCAAGAGATTCTGTCGGCCATGGAACTCACACTGCATCCATAGCAGCCGGTGTGCAAGTTCCCAATGCCAGTTACTATGGCTTGGCACAAGGTACAGCAAAGGGAGGCTCACCGTCAGCTAGGATTGCATGCTACAAGGCATGCTTGGATGTTGGTTGCTCTGGTGCCACCATATTGAAGGCTATCGATGATGCAGTTAGAGATGGAGTAGACGTCATATCTATTTCCATTGGGATGAGCTCACTGTTTCAGTCTGACTACTTAAATGACCCCATAGCCATCGGAGCATTTCACGCTGAGCAAATGGGAGTGATGGTAATTTGCTCTGCTGGAAATGATGGTCCCAATCCTTACACTATTGTAAACACAGCACCATGGATCTTCACCGTTGCAGCTTCTAACATTGATAGGGATTTCCAATCTAATATCATTCTTGGAAATGGGAGAACATTCACTGGTTCTGCCATCAATTTCTCCAATCTCACTCGTTCAAAAACATATCCCCTTGTATTTGGAAAAGATGCCGCTGGCATTAACACGCCTGTATCGGAAGCAAGAAATTGCTATCCGGGATCATTGGATCAAAAGAAAGTAGTTGGCAAAATAGTCGTTTGTGTTGATGATGACCCGGCTGTTTCACGGAGAACCAAGAAATTAGTTGTTGCTGATACTAAAGCCAAAGGGTTGATTTTGATTGATGAAGCTGAGAAAGGTGTACCTTTCGATTCAGGCATTTTCCCATTTACAGAAGTTGGCAATATTGCAGGGCTTCAGATTCTCAAGTACATAAATTCTACCCAAAAACCAACAGCAACGATTCTCCCAACAGTTGTAGTTCCACAGTATAGACCTGCACCGGCCGTTGCATTTTTTTCATCGAGGGGTCCTGCACAGCTTACAGAAAACATTCTCAAGCCTGATATAATGGCTCCAGGAGTGGCCGTTTTAGCTGCCATAGCTCCCAAGAATGAAACAGGGACAGTCCCAAATGGGAAGAAGCCATCAAAATTTTCCATAAAATCCGGGACATCAATGGCTTGTCCACACGTAACGGGGACTGCTGCATTCATCAAGTCAGTGCATCGTCAATGGACTACTTCCATCGATCAAATCAGCCCTTATGACAACAGCAACCATTTACAACAACATGAAAATGCCTTTGACAGACAACTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

496

Amino Acids

52.84

Weight (kDa)

8.44

Isoelectric Point (pI)

39.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 30 - 470 1.1e-40 Subtilase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 98
AatII GACGTC 1 cut(s) 528
Acc36I ACCTGC 1 cut(s) 1171
AccB1I GGYRCC 1 cut(s) 476
AccI GTMKAC 1 cut(s) 522
AciI CCGC 1 cut(s) 837
AclWI GGATC 3 cut(s) 698, 892, 900
AcoI YGGCCR 4 cut(s) 51, 328, 1170, 1251
AcsI RAATTY 2 cut(s) 1102, 1319
AcuI CTGAAG 2 cut(s) 127, 1067
AcyI GRCGYC 1 cut(s) 525
AfaI GTAC 4 cut(s) 301, 406, 1029, 1097
AflIII ACRYGT 1 cut(s) 1357
AgsI TTSAA 5 cut(s) 64, 174, 211, 490, 804
AjnI CCWGG 2 cut(s) 53, 1243
AluBI AGCT 8 cut(s) 141, 431, 552, 707, 1016, 1207, 1262, 1271
AluI AGCT 8 cut(s) 141, 431, 552, 707, 1016, 1207, 1262, 1271
Alw21I GWGCWC 1 cut(s) 554
Alw26I GTCTC 2 cut(s) 35, 138
AlwI GGATC 3 cut(s) 698, 892, 900
AoxI GGCC 6 cut(s) 51, 56, 125, 328, 1170, 1251
ApeKI GCWGC 4 cut(s) 356, 704, 1262, 1373
ApoI RAATTY 2 cut(s) 1102, 1319
Asp700I GAANNNNTTC 1 cut(s) 1218
AspS9I GGNCC 2 cut(s) 653, 1196
AsuC2I CCSGG 3 cut(s) 882, 941, 1336
AsuHPI GGTGA 5 cut(s) 277, 290, 303, 414, 688
AvaII GGWCC 2 cut(s) 653, 1196
BaeI ACNNNNGTAYC 2 cut(s) 840, 873
BalI TGGCCA 1 cut(s) 53
BanI GGYRCC 1 cut(s) 476
BanII GRGCYC 1 cut(s) 554
Bbv12I GWGCWC 1 cut(s) 554
BbvI GCAGC 4 cut(s) 368, 716, 1249, 1360
BccI CCATC 9 cut(s) 91, 171, 509, 599, 619, 644, 785, 1321, 1421
BceAI ACGGC 2 cut(s) 1157, 1238
BcgI CGANNNNNNTGC 2 cut(s) 901, 935
BciT130I CCWGG 2 cut(s) 55, 1245
BcnI CCSGG 3 cut(s) 882, 941, 1336
BcoDI GTCTC 2 cut(s) 35, 138
BfaI CTAG 1 cut(s) 432
BfuAI ACCTGC 1 cut(s) 1171
BisI GCNGC 5 cut(s) 357, 705, 837, 1263, 1374
BlpI GCTNAGC 1 cut(s) 609
BlsI GCNGC 5 cut(s) 358, 706, 838, 1264, 1375
Bme1390I CCNGG 5 cut(s) 55, 882, 941, 1245, 1336
Bme18I GGWCC 2 cut(s) 653, 1196
BmgT120I GGNCC 2 cut(s) 653, 1196
BmiI GGNNCC 4 cut(s) 478, 655, 1197, 1242
BmrFI CCNGG 5 cut(s) 55, 882, 941, 1245, 1336
BmsI GCATC 4 cut(s) 356, 493, 823, 1402
BpmI CTGGAG 1 cut(s) 1227
Bpu1102I GCTNAGC 1 cut(s) 609
BpuEI CTTGAG 2 cut(s) 1076, 1208
BpuMI CCSGG 3 cut(s) 882, 941, 1336
Bsa29I ATCGAT 2 cut(s) 498, 1416
BsaAI YACGTR 1 cut(s) 1360
BsaHI GRCGYC 1 cut(s) 525
BsaJI CCNNGG 2 cut(s) 331, 686
BsaWI WCCGGW 1 cut(s) 262
BsaXI ACNNNNNCTCC 6 cut(s) 510, 540, 612, 642, 1239, 1269
Bse118I RCCGGY 2 cut(s) 359, 1168
Bse1I ACTGG 4 cut(s) 297, 303, 381, 772
BseBI CCWGG 2 cut(s) 55, 1245
BseCI ATCGAT 2 cut(s) 498, 1416
BseDI CCNNGG 2 cut(s) 331, 686
BseGI GGATG 4 cut(s) 111, 347, 466, 552
BseMII CTCAG 3 cut(s) 120, 600, 1008
BseNI ACTGG 4 cut(s) 297, 303, 381, 772
BseX3I CGGCCG 1 cut(s) 1170
BseXI GCAGC 4 cut(s) 368, 716, 1249, 1360
BsgI GTGCAG 4 cut(s) 62, 183, 1149, 1185
Bsh1285I CGRYCG 1 cut(s) 1173
BshFI GGCC 6 cut(s) 53, 58, 127, 330, 1172, 1253
BshNI GGYRCC 1 cut(s) 476
BshVI ATCGAT 2 cut(s) 498, 1416
BsiEI CGRYCG 1 cut(s) 1173
BsiHKAI GWGCWC 1 cut(s) 554
BsiSI CCGG 6 cut(s) 263, 360, 881, 941, 1169, 1335
BslFI GGGAC 6 cut(s) 152, 639, 1280, 1303, 1351, 1381
BsmAI GTCTC 2 cut(s) 35, 138
BsmBI CGTCTC 1 cut(s) 35
BsmFI GGGAC 6 cut(s) 152, 639, 1280, 1303, 1351, 1381
BsmI GAATGC 1 cut(s) 1376
BsnI GGCC 6 cut(s) 53, 58, 127, 330, 1172, 1253
Bsp1286I GDGCHC 1 cut(s) 554
Bsp143I GATC 4 cut(s) 690, 884, 892, 1417
Bsp1720I GCTNAGC 1 cut(s) 609
Bsp19I CCATGG 2 cut(s) 331, 686
BspACI CCGC 1 cut(s) 837
BspANI GGCC 6 cut(s) 53, 58, 127, 330, 1172, 1253
BspCNI CTCAG 3 cut(s) 121, 601, 1009
BspDI ATCGAT 2 cut(s) 498, 1416
BspLI GGNNCC 4 cut(s) 478, 655, 1197, 1242
BspMI ACCTGC 1 cut(s) 1171
BspPI GGATC 3 cut(s) 698, 892, 900
BspT107I GGYRCC 1 cut(s) 476
BsrFI RCCGGY 2 cut(s) 359, 1168
BsrI ACTGG 4 cut(s) 297, 303, 381, 772
BssAI RCCGGY 2 cut(s) 359, 1168
BssECI CCNNGG 2 cut(s) 331, 686
BssMI GATC 4 cut(s) 690, 884, 892, 1417
BssNI GRCGYC 1 cut(s) 525
BssT1I CCWWGG 2 cut(s) 331, 686
Bst2UI CCWGG 2 cut(s) 55, 1245
Bst4CI ACNGT 6 cut(s) 426, 558, 700, 1141, 1155, 1294
BstACI GRCGYC 1 cut(s) 525
BstBAI YACGTR 1 cut(s) 1360
BstC8I GCNNGC 3 cut(s) 442, 454, 841
BstDEI CTNAG 3 cut(s) 129, 609, 1017
BstDSI CCRYGG 2 cut(s) 331, 686
BstEII GGTNACC 1 cut(s) 278
BstF5I GGATG 4 cut(s) 111, 347, 466, 552
BstKTI GATC 4 cut(s) 693, 887, 895, 1420
BstMAI GTCTC 2 cut(s) 35, 138
BstMBI GATC 4 cut(s) 690, 884, 892, 1417
BstMCI CGRYCG 1 cut(s) 1173
BstMWI GCNNNNNNNGC 6 cut(s) 353, 437, 596, 605, 1259, 1268
BstNI CCWGG 2 cut(s) 55, 1245
BstNSI RCATGY 2 cut(s) 444, 456
BstPI GGTNACC 1 cut(s) 278
BstSCI CCNGG 5 cut(s) 53, 880, 939, 1243, 1334
BstV1I GCAGC 4 cut(s) 368, 716, 1249, 1360
BstX2I RGATCY 1 cut(s) 690
BstYI RGATCY 1 cut(s) 690
BstZI CGGCCG 1 cut(s) 1170
Bsu15I ATCGAT 2 cut(s) 498, 1416
BsuRI GGCC 6 cut(s) 53, 58, 127, 330, 1172, 1253
BsuTUI ATCGAT 2 cut(s) 498, 1416
BtgI CCRYGG 2 cut(s) 331, 686
BtsCI GGATG 4 cut(s) 111, 347, 466, 552
BtsI GCAGTG 1 cut(s) 342
BtsIMutI CAGTG 6 cut(s) 151, 310, 342, 554, 765, 1395
BveI ACCTGC 1 cut(s) 1171
Cac8I GCNNGC 3 cut(s) 442, 454, 841
Cfr10I RCCGGY 2 cut(s) 359, 1168
Cfr13I GGNCC 2 cut(s) 653, 1196
ClaI ATCGAT 2 cut(s) 498, 1416
Csp6I GTAC 4 cut(s) 300, 405, 1028, 1096
CviAII CATG 5 cut(s) 332, 441, 453, 687, 1460
CviQI GTAC 4 cut(s) 300, 405, 1028, 1096
DdeI CTNAG 3 cut(s) 129, 609, 1017
DpnI GATC 4 cut(s) 692, 886, 894, 1419
DpnII GATC 4 cut(s) 690, 884, 892, 1417
EaeI YGGCCR 4 cut(s) 51, 328, 1170, 1251
EagI CGGCCG 1 cut(s) 1170
Ecl136II GAGCTC 1 cut(s) 552
EclXI CGGCCG 1 cut(s) 1170
Eco130I CCWWGG 2 cut(s) 331, 686
Eco24I GRGCYC 1 cut(s) 554
Eco47I GGWCC 2 cut(s) 653, 1196
Eco52I CGGCCG 1 cut(s) 1170
Eco53kI GAGCTC 1 cut(s) 552
Eco57I CTGAAG 2 cut(s) 127, 1067
Eco91I GGTNACC 1 cut(s) 278
EcoICRI GAGCTC 1 cut(s) 552
EcoO109I RGGNCCY 1 cut(s) 1196
EcoO65I GGTNACC 1 cut(s) 278
EcoRII CCWGG 2 cut(s) 53, 1243
EcoT14I CCWWGG 2 cut(s) 331, 686
EcoT38I GRGCYC 1 cut(s) 554
ErhI CCWWGG 2 cut(s) 331, 686
Esp3I CGTCTC 1 cut(s) 35
FaeI CATG 5 cut(s) 335, 444, 456, 690, 1463
FalI AAGNNNNNCTT 2 cut(s) 440, 472
FaqI GGGAC 6 cut(s) 152, 639, 1280, 1303, 1351, 1381
FatI CATG 5 cut(s) 331, 440, 452, 686, 1459
FblI GTMKAC 1 cut(s) 522
Fnu4HI GCNGC 5 cut(s) 357, 705, 837, 1263, 1374
FokI GGATG 4 cut(s) 118, 334, 473, 559
FriOI GRGCYC 1 cut(s) 554
Fsp4HI GCNGC 5 cut(s) 357, 705, 837, 1263, 1374
FspBI CTAG 1 cut(s) 432
GluI GCNGC 5 cut(s) 357, 705, 837, 1263, 1374
GsuI CTGGAG 1 cut(s) 1227
HaeIII GGCC 6 cut(s) 53, 58, 127, 330, 1172, 1253
HapII CCGG 6 cut(s) 263, 360, 881, 941, 1169, 1335
Hin1I GRCGYC 1 cut(s) 525
Hin1II CATG 5 cut(s) 335, 444, 456, 690, 1463
HinfI GANTC 6 cut(s) 131, 186, 320, 1037, 1087, 1129
HpaII CCGG 6 cut(s) 263, 360, 881, 941, 1169, 1335
HphI GGTGA 5 cut(s) 277, 290, 303, 414, 688
Hpy166II GTNNAC 4 cut(s) 523, 676, 1028, 1355
Hpy188I TCNGA 5 cut(s) 138, 568, 596, 862, 1086
Hpy188III TCNNGA 3 cut(s) 64, 174, 211
Hpy8I GTNNAC 4 cut(s) 523, 676, 1028, 1355
HpyAV CCTTC 3 cut(s) 180, 191, 484
HpyCH4III ACNGT 6 cut(s) 426, 558, 700, 1141, 1155, 1294
HpyCH4IV ACGT 2 cut(s) 525, 1359
HpyF10VI GCNNNNNNNGC 6 cut(s) 353, 437, 596, 605, 1259, 1268
HpyF3I CTNAG 3 cut(s) 129, 609, 1017
HpySE526I ACGT 2 cut(s) 525, 1359
Hsp92I GRCGYC 1 cut(s) 525
Hsp92II CATG 5 cut(s) 335, 444, 456, 690, 1463
Kzo9I GATC 4 cut(s) 690, 884, 892, 1417
LmnI GCTCC 4 cut(s) 9, 596, 1246, 1276
Lsp1109I GCAGC 4 cut(s) 368, 716, 1249, 1360
LweI GCATC 4 cut(s) 356, 493, 823, 1402
MaeI CTAG 1 cut(s) 432
MaeII ACGT 2 cut(s) 525, 1359
MaeIII GTNAC 4 cut(s) 265, 278, 383, 1360
MalI GATC 4 cut(s) 692, 886, 894, 1419
MboI GATC 4 cut(s) 690, 884, 892, 1417
MboII GAAGA 2 cut(s) 685, 1318
MflI RGATCY 1 cut(s) 690
MhlI GDGCHC 1 cut(s) 554
MlsI TGGCCA 1 cut(s) 53
MluNI TGGCCA 1 cut(s) 53
MlyI GAGTC 2 cut(s) 140, 195
MmeI TCCRAC 1 cut(s) 31
MnlI CCTC 3 cut(s) 145, 410, 1185
Mox20I TGGCCA 1 cut(s) 53
MroXI GAANNNNTTC 1 cut(s) 1218
MscI TGGCCA 1 cut(s) 53
MseI TTAA 2 cut(s) 575, 846
MslI CAYNNNNRTG 2 cut(s) 545, 1464
Msp20I TGGCCA 1 cut(s) 53
MspA1I CMGCKG 1 cut(s) 839
MspI CCGG 6 cut(s) 263, 360, 881, 941, 1169, 1335
MspR9I CCNGG 5 cut(s) 55, 882, 941, 1245, 1336
Mva1269I GAATGC 1 cut(s) 1376
MvaI CCWGG 2 cut(s) 55, 1245
MwoI GCNNNNNNNGC 6 cut(s) 353, 437, 596, 605, 1259, 1268
NciI CCSGG 3 cut(s) 882, 941, 1336
NcoI CCATGG 2 cut(s) 331, 686
NdeII GATC 4 cut(s) 690, 884, 892, 1417
NlaIII CATG 5 cut(s) 335, 444, 456, 690, 1463
NlaIV GGNNCC 4 cut(s) 478, 655, 1197, 1242
NmuCI GTSAC 2 cut(s) 265, 278
NspI RCATGY 2 cut(s) 444, 456
PaeI GCATGC 2 cut(s) 444, 456
PctI GAATGC 1 cut(s) 1376
PdmI GAANNNNTTC 1 cut(s) 1218
PfeI GAWTC 4 cut(s) 320, 1037, 1087, 1129
PfoI TCCNGGA 3 cut(s) 880, 1243, 1334
PkrI GCNGC 5 cut(s) 358, 706, 838, 1264, 1375
PleI GAGTC 2 cut(s) 139, 194
PpsI GAGTC 2 cut(s) 139, 194
Ppu21I YACGTR 1 cut(s) 1360
PpuMI RGGWCCY 1 cut(s) 1196
PsiI TTATAA 1 cut(s) 98
Psp124BI GAGCTC 1 cut(s) 554
Psp5II RGGWCCY 1 cut(s) 1196
Psp6I CCWGG 2 cut(s) 53, 1243
PspEI GGTNACC 1 cut(s) 278
PspGI CCWGG 2 cut(s) 53, 1243
PspN4I GGNNCC 4 cut(s) 478, 655, 1197, 1242
PspPI GGNCC 2 cut(s) 653, 1196
PspPPI RGGWCCY 1 cut(s) 1196
PsuI RGATCY 1 cut(s) 690
RsaI GTAC 4 cut(s) 301, 406, 1029, 1097
RsaNI GTAC 4 cut(s) 300, 405, 1028, 1096
RseI CAYNNNNRTG 2 cut(s) 545, 1464
SacI GAGCTC 1 cut(s) 554
SaqAI TTAA 2 cut(s) 575, 846
SatI GCNGC 5 cut(s) 357, 705, 837, 1263, 1374
Sau3AI GATC 4 cut(s) 690, 884, 892, 1417
Sau96I GGNCC 2 cut(s) 653, 1196
SchI GAGTC 2 cut(s) 140, 195
ScrFI CCNGG 5 cut(s) 55, 882, 941, 1245, 1336
SduI GDGCHC 1 cut(s) 554
SfaNI GCATC 4 cut(s) 356, 493, 823, 1402
SinI GGWCC 2 cut(s) 653, 1196
SmiMI CAYNNNNRTG 2 cut(s) 545, 1464
SmlI CTYRAG 2 cut(s) 1091, 1223
SmoI CTYRAG 2 cut(s) 1091, 1223
SphI GCATGC 2 cut(s) 444, 456
SsiI CCGC 1 cut(s) 837
SspI AATATT 1 cut(s) 1072
SspMI CTAG 1 cut(s) 432
SstI GAGCTC 1 cut(s) 554
StyD4I CCNGG 5 cut(s) 53, 880, 939, 1243, 1334
StyI CCWWGG 2 cut(s) 331, 686
TaaI ACNGT 6 cut(s) 426, 558, 700, 1141, 1155, 1294
TaiI ACGT 2 cut(s) 528, 1362
TaqI TCGA 4 cut(s) 498, 1035, 1190, 1416
TatI WGTACW 1 cut(s) 1095
TauI GCSGC 1 cut(s) 839
TfiI GAWTC 4 cut(s) 320, 1037, 1087, 1129
Tru1I TTAA 2 cut(s) 575, 846
Tru9I TTAA 2 cut(s) 575, 846
TscAI CASTG 6 cut(s) 151, 310, 349, 561, 772, 1395
TseFI GTSAC 2 cut(s) 265, 278
TseI GCWGC 4 cut(s) 356, 704, 1262, 1373
Tsp45I GTSAC 2 cut(s) 265, 278
TspDTI ATGAA 5 cut(s) 1026, 1176, 1296, 1369, 1476
TspGWI ACGGA 2 cut(s) 59, 967
TspRI CASTG 6 cut(s) 151, 310, 349, 561, 772, 1395
VpaK11BI GGWCC 2 cut(s) 653, 1196
XapI RAATTY 2 cut(s) 1102, 1319
XceI RCATGY 2 cut(s) 444, 456
XmiI GTMKAC 1 cut(s) 522
XmnI GAANNNNTTC 1 cut(s) 1218
XspI CTAG 1 cut(s) 432
ZraI GACGTC 1 cut(s) 526
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.