pycom02g23660

breast cancer carboxy-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0012645
Physical Location & Seq
Forward (+)
21813023 .. 21820567
7545 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3291 bp
ATGTCTCATTCTGTCGACACGCTAGGGTTTCGTCCTCCACAGTTCTCCGAGGATTTAGCTTGGCTTCCTGGTTGGCTTCAGCAGCACCAGAAAGAACAATTGGATGAATGCACCAACGAACTTAAGGGTACCAACTTAGAGCTAGCGTCCAAGGATTTGAGAATTTTTCAAGGAAACACTAGTGAAGGAAAAGATGCTAATACATTTTCACACGAGGAAGTTGGGTACAAAAGCTGTCATTTATTCTTATCTGGGGAAGACAATTCTGCAGTTAGCTTTGCTTCATCTCCTGGAAATGTACTTCATTTCCATCTTCATCTTTCATCAAATGGATATTCACAATGTAGCCCACTTCAACCTTTGGATGCTTCTCAAAATCATCTTGAATCCAATACAGTTCTCTCCGTGCAACTAAATAACACCTCAGTGGGTTCTGAATTGAAGAGCTGCTCCAAAATTGGCCTAAATGTTGGTGGGATAAATTCTTTACCTCCAAAGTCCATTGAAAAACCCAGGGAGGATACTGTTCCACCCTGCCCCTCCAATAACAAGAAATCAGCAAGTCATTCTGATGAAAAGCTTGATACGAGGTACCTGAAAGCAGCTGACATCTCTGATGCAGTTGAACTCTCCATTGCAGCATCTGAAGCATTGGTTATAAATGAAATAATGGGGAGTGGCTTGCCTTCGGACGTATTGCCAACAGCAGTGGTACTGGAAGCAGCCCTTCAAGTAAAGAAAGCAAGATTGGAGTGGCTAGATGATTCCTTAGATGGCCCAGCTGAGGAAACTGAAAATTGTGATTCTCTCTCAGATTTGGATGATTTCAACATGGCCGATGTATATAAAGATGTAGGGCTGTCTCAGAGCATACCTTCTGATGAGTGTGCATGTGATTCAGCTATTTCCCAAGTGAAAGAGACCCCTCTCTCTGGAATTCTGTATGAGTGTGTAAATCTATCTGATTCTTCAGAGCTCAGGGCTCAATGTGTCAAGTTTGATGATATCCCTATGCAAAAAGAACTAGGACAGAATTTGGTTATGGATCTCAAATCAACTGAGAACTTCCATCCAGAATCCGTCAACTATGAGAGGGAACAGCTTCATGATAAGCTTGTTCTGGGTTCAAATATCAGTGTGGCCAGATATGATCCTTCAGCTCTAAAAAATTCAGATGGTTTTATCATGAAGCAGACAGTTGGCGCTATGGTGGATGTAGCATCATTTCAGCCTCAAAATAATGTAAACTTCCGCCCACAAGCTTGGAATTCAGGGAACTCTAAGGGAGAAGATACAGTAAGTTACTTAGCTTCGAACAGATTCAGGAGCCGGTGGTTAGGGGGTTGGACAGGCCAGGATGCATCTGCTACTCCAGAATTGAAACAGAATACTAAAAGTGTGATGAAGTGTTTTGCTGGTGAGACGAGTTTTTTCTCAGAATCGGCAGATATTGCTCCAGACGTAAACTCTTTTGTGCAAGTGCATGACACCAAGTTTTATAGAACATCACAGTCAAGCATAGCCTTTTCTGGTTTACATGACGAAGATAACAATGGGATAATGCTCTCTCAAGATGTGGGGACATCTTCTAGCCTATCTTCAGTAGATCCTCTTTGTTCCGTTGTCCCGTGCAGTATTTCTTCAGAAAATGCTAGTCTTACATTAGCTCACAATCAGAAGGATAAGGAAAATCATAATGAAGAATGCTTCAGACCCACACAGCAACTTGCGGTGGAGAATTCACACAAATCGTCAAATCCAATTATTAAATTTCCCCATGAGGACGGGCCATCTATGCCCACAATTAATGGTGAGCGTTCTCCAGTCACAGTTCGAAGGAAAATGATTTCACTCAGGACATATAGTACGCTTCTCCCAAACCTTGTTTCCATTTTCGATGGAGGAAGCTTTTATCGTGATCGGTCATTTGAACTAGAACTCGATCAGAGGCTGAATCCCTTGAATAAGGATGTCCCTTGCGATAGATCTTCTGATAAAAGGAGTTATAAAGAGTCGCTTCCTTCCAATACTGTATCCTCATATTCTACTGGAAGAGACAATGAGGGAAACAGTGAAACTACCTTAGATGCGAATCCTGTTGCAACACTGAAAGATCAGAAGAGAAGTTATCACGAGACTGAAGGAAATGGGAACGAGCTACCTATTCAAGCATTGAAAAAGAGGAGGCAACCTCTTATTTTTAATCATAGGTCACGTTTCCGTATCCAGGCTTCTAAACCTTTAATGAACAATTCCACTTTGGAGAAACATCTCAAACTTGATTTGCTGCCGGAAAATGTTGTTAAGCTTCAACAAAATGAGGAACTCCACACTATACAATCTGCATGCAAGAACTTCCCTGACAGAGATGTTTTAGTGAAAAAGAGAGTTCATTTCTGTGAAGCAGATATTGCAGTTCAGCAGAACAAGAACCTCCAAAAGCTAGATTCTTCAACCAAATATTGCTCAAATGCTAGAGCAAGTAAAAGATGGAAACATCCCAAGTTTCAAAGTCATGAGAGAAGCAGCCGTACAAATTGTCACCTTAAGTCTGGGAAGAGATTACTGTTTCATGGTATAGAATTCCTGCTTACAGGATTTTCTAGTCAGAAGGAAAAGGACGTTGAACGACAAATATGGAAACATGGTGGCATTGTTCTTTCTGATATTCCTTCTCCAAATTTAAGGGCAAAAAGAAGCTTACGATCCAATGGCTACCATCTTCCCGTTATTCTATGTATGAATAAGCTACAGACCACCAAGTTTTTGTATGGTTGTGCAGTGAACTCCTTAATATTAAAAGTTGATTGGCTTACTAATTCAATTTCGGCAGGCTGTATTTTACTACCTGAGAAGAAATACATGATACTACCGAATCGAGCTGATGCAGAGCATATCATTATTGGGGAGCCATTTCACCACAACCGCAATTATGTCTTTGAAAAAGTAGGAATCATGCTTCACGGAAAGCACAGTTTCTACAGCAAATTGGCAAAAATAATTAAGCACGGAGGTGGGAAGGTCTTTAAAACCCTCCAGTGGTTAGTCCACAGTTTGGACAAAGAGAAAGTTACTTTGGGAGCCATTGTTGCTGAGGATGAAGGTAGGACATCACGTCACTTGAGGCAGTGCGCATCCGAGCAGAAGATAGCAGTGATGCCAGTTAGCTGGATCATAAAAGGCTTGTATTTAGGTAAGCTGCTTCCGTTTCCAGATAAAGGTCGGCCCGCTTTACCAACAATTAAGATCTCAGAGATCCCAGTTTCCGCAAAAGTGAGTGAAGATTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BRCT_2 RTT107_BRCT_5

Protein Analysis

1097

Amino Acids

121.95

Weight (kDa)

6.49

Isoelectric Point (pI)

48.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BRCT_3 PF18428 979 - 1071 2e-06 BRCA1 C Terminus (BRCT) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 659, 2007
Acc16I TGCGCA 1 cut(s) 3133
Acc65I GGTACC 2 cut(s) 128, 591
AccB1I GGYRCC 2 cut(s) 128, 591
AccB7I CCANNNNNTGG 1 cut(s) 3055
AccI GTMKAC 1 cut(s) 15
AciI CCGC 5 cut(s) 1252, 1730, 2926, 3228, 3267
AclWI GGATC 6 cut(s) 1053, 1145, 1601, 2700, 3179, 3250
AcoI YGGCCR 2 cut(s) 834, 1140
AcuI CTGAAG 8 cut(s) 62, 666, 954, 1140, 1584, 1626, 1693, 2160
AfaI GTAC 7 cut(s) 130, 227, 300, 593, 714, 1867, 2533
AfiI CCNNNNNNNGG 6 cut(s) 784, 932, 2227, 3039, 3055, 3218
AflII CTTAAG 2 cut(s) 122, 2546
AhdI GACNNNNNGTC 1 cut(s) 3114
AhlI ACTAGT 1 cut(s) 179
AjiI CACGTC 1 cut(s) 3116
AjnI CCWGG 5 cut(s) 67, 289, 512, 1353, 2226
AjuI GAANNNNNNNTTGG 8 cut(s) 83, 115, 731, 763, 1870, 1902, 3059, 3091
AleI CACNNNNGTG 2 cut(s) 425, 3012
Alw21I GWGCWC 1 cut(s) 978
Alw26I GTCTC 6 cut(s) 9, 867, 914, 1415, 2049, 2129
AlwI GGATC 6 cut(s) 1053, 1145, 1601, 2700, 3179, 3250
AlwNI CAGNNNCTG 2 cut(s) 644, 1951
AoxI GGCC 7 cut(s) 460, 775, 834, 1140, 1351, 1787, 3224
ApeKI GCWGC 8 cut(s) 82, 447, 602, 638, 722, 2287, 2526, 3199
AseI ATTAAT 1 cut(s) 1806
Asp700I GAANNNNTTC 2 cut(s) 1101, 1319
Asp718I GGTACC 2 cut(s) 128, 591
AspLEI GCGC 2 cut(s) 1205, 3134
AspS9I GGNCC 3 cut(s) 776, 1787, 3225
AsuHPI GGTGA 4 cut(s) 1430, 1823, 2534, 2909
AsuII TTCGAA 2 cut(s) 1313, 1834
AsuNHI GCTAGC 1 cut(s) 142
BalI TGGCCA 1 cut(s) 1142
BanI GGYRCC 2 cut(s) 128, 591
BanII GRGCYC 2 cut(s) 978, 985
BarI GAAGNNNNNNTAC 4 cut(s) 2215, 2247, 2515, 2547
BauI CACGAG 2 cut(s) 212, 2132
BbsI GAAGAC 1 cut(s) 264
Bbv12I GWGCWC 1 cut(s) 978
BbvCI CCTCAGC 2 cut(s) 783, 3093
BbvI GCAGC 8 cut(s) 94, 434, 614, 650, 734, 2274, 2538, 3186
BccI CCATC 8 cut(s) 318, 767, 1077, 1169, 1798, 1892, 2484, 2727
BceAI ACGGC 1 cut(s) 2514
BciT130I CCWGG 5 cut(s) 69, 291, 514, 1355, 2228
BciVI GTATCC 3 cut(s) 514, 2044, 2234
BcoDI GTCTC 6 cut(s) 9, 867, 914, 1415, 2049, 2129
BcuI ACTAGT 1 cut(s) 179
BfmI CTRYAG 3 cut(s) 267, 2750, 2980
BfoI RGCGCY 1 cut(s) 1206
BfrI CTTAAG 2 cut(s) 122, 2546
BfuI GTATCC 3 cut(s) 514, 2044, 2234
BglII AGATCT 2 cut(s) 1985, 3246
BisI GCNGC 8 cut(s) 83, 448, 603, 639, 723, 2288, 2527, 3200
BlsI GCNGC 8 cut(s) 84, 449, 604, 640, 724, 2289, 2528, 3201
Bme1390I CCNGG 5 cut(s) 69, 291, 514, 1355, 2228
BmeRI GACNNNNNGTC 1 cut(s) 3114
BmgBI CACGTC 1 cut(s) 3116
BmgT120I GGNCC 3 cut(s) 776, 1787, 3225
BmiI GGNNCC 5 cut(s) 130, 593, 1328, 2910, 3082
BmrFI CCNGG 5 cut(s) 69, 291, 514, 1355, 2228
BmrI ACTGGG 1 cut(s) 3254
BmtI GCTAGC 1 cut(s) 146
BmuI ACTGGG 1 cut(s) 3254
BpiI GAAGAC 1 cut(s) 264
BplI GAGNNNNNCTC 2 cut(s) 2176, 2208
BpmI CTGGAG 4 cut(s) 1356, 1440, 1806, 3020
Bpu10I CCTNAGC 3 cut(s) 783, 977, 3093
Bpu14I TTCGAA 2 cut(s) 1313, 1834
BpuEI CTTGAG 2 cut(s) 1554, 3142
BsaBI GATNNNNATC 1 cut(s) 2091
BsaI GGTCTC 1 cut(s) 914
BsaJI CCNNGG 4 cut(s) 48, 150, 512, 513
BsaXI ACNNNNNCTCC 2 cut(s) 3072, 3102
Bsc4I CCNNNNNNNGG 6 cut(s) 784, 932, 2227, 3039, 3055, 3218
Bse118I RCCGGY 1 cut(s) 1329
Bse1I ACTGG 6 cut(s) 720, 1823, 2053, 3037, 3161, 3260
Bse3DI GCAATG 1 cut(s) 633
Bse8I GATNNNNATC 1 cut(s) 2091
BseBI CCWGG 5 cut(s) 69, 291, 514, 1355, 2228
BseDI CCNNGG 4 cut(s) 48, 150, 512, 513
BseJI GATNNNNATC 1 cut(s) 2091
BseLI CCNNNNNNNGG 6 cut(s) 784, 932, 2227, 3039, 3055, 3218
BseMI GCAATG 1 cut(s) 633
BseNI ACTGG 6 cut(s) 720, 1823, 2053, 3037, 3161, 3260
BseRI GAGGAG 1 cut(s) 2197
BseXI GCAGC 8 cut(s) 94, 434, 614, 650, 734, 2274, 2538, 3186
BseYI CCCAGC 1 cut(s) 778
BsgI GTGCAG 2 cut(s) 1651, 2799
BshFI GGCC 7 cut(s) 462, 777, 836, 1142, 1353, 1789, 3226
BshNI GGYRCC 2 cut(s) 128, 591
BsiHKAI GWGCWC 1 cut(s) 978
BsiSI CCGG 2 cut(s) 1330, 2291
BslFI GGGAC 3 cut(s) 1594, 1610, 1958
BslI CCNNNNNNNGG 6 cut(s) 784, 932, 2227, 3039, 3055, 3218
BsmAI GTCTC 6 cut(s) 9, 867, 914, 1415, 2049, 2129
BsmBI CGTCTC 1 cut(s) 1415
BsmFI GGGAC 3 cut(s) 1594, 1610, 1958
BsmI GAATGC 2 cut(s) 113, 1709
BsnI GGCC 7 cut(s) 462, 777, 836, 1142, 1353, 1789, 3226
Bso31I GGTCTC 1 cut(s) 914
Bsp119I TTCGAA 2 cut(s) 1313, 1834
Bsp1286I GDGCHC 2 cut(s) 978, 985
BspACI CCGC 5 cut(s) 1252, 1730, 2926, 3228, 3267
BspANI GGCC 7 cut(s) 462, 777, 836, 1142, 1353, 1789, 3226
BspHI TCATGA 3 cut(s) 1105, 1185, 2515
BspLI GGNNCC 5 cut(s) 130, 593, 1328, 2910, 3082
BspMAI CTGCAG 1 cut(s) 271
BspOI GCTAGC 1 cut(s) 146
BspPI GGATC 6 cut(s) 1053, 1145, 1601, 2700, 3179, 3250
BspQI GCTCTTC 1 cut(s) 437
BspT104I TTCGAA 2 cut(s) 1313, 1834
BspT107I GGYRCC 2 cut(s) 128, 591
BspTI CTTAAG 2 cut(s) 122, 2546
BspTNI GGTCTC 1 cut(s) 914
BsrDI GCAATG 1 cut(s) 633
BsrFI RCCGGY 1 cut(s) 1329
BsrI ACTGG 6 cut(s) 720, 1823, 2053, 3037, 3161, 3260
BssAI RCCGGY 1 cut(s) 1329
BssECI CCNNGG 4 cut(s) 48, 150, 512, 513
BssSI CACGAG 2 cut(s) 212, 2132
BssT1I CCWWGG 1 cut(s) 150
Bst2BI CACGAG 2 cut(s) 212, 2132
Bst2UI CCWGG 5 cut(s) 69, 291, 514, 1355, 2228
Bst6I CTCTTC 4 cut(s) 437, 2047, 2114, 2552
BstAFI CTTAAG 2 cut(s) 122, 2546
BstAPI GCANNNNNTGC 2 cut(s) 1451, 2411
BstBI TTCGAA 2 cut(s) 1313, 1834
BstC8I GCNNGC 5 cut(s) 144, 683, 2347, 2833, 3228
BstH2I RGCGCY 1 cut(s) 1206
BstHHI GCGC 2 cut(s) 1205, 3134
BstMAI GTCTC 6 cut(s) 9, 867, 914, 1415, 2049, 2129
BstMWI GCNNNNNNNGC 6 cut(s) 82, 647, 1451, 1795, 2411, 3089
BstNI CCWGG 5 cut(s) 69, 291, 514, 1355, 2228
BstNSI RCATGY 2 cut(s) 894, 2349
BstSCI CCNGG 5 cut(s) 67, 289, 512, 1353, 2226
BstSFI CTRYAG 3 cut(s) 267, 2750, 2980
BstV1I GCAGC 8 cut(s) 94, 434, 614, 650, 734, 2274, 2538, 3186
BstV2I GAAGAC 1 cut(s) 264
BstX2I RGATCY 5 cut(s) 1045, 1606, 1985, 3246, 3255
BstXI CCANNNNNNTGG 2 cut(s) 1263, 3168
BstYI RGATCY 5 cut(s) 1045, 1606, 1985, 3246, 3255
BsuI GTATCC 3 cut(s) 514, 2044, 2234
BsuRI GGCC 7 cut(s) 462, 777, 836, 1142, 1353, 1789, 3226
BtrI CACGTC 1 cut(s) 3116
BtsI GCAGTG 4 cut(s) 714, 2787, 3134, 3159
BtsIMutI CAGTG 9 cut(s) 432, 714, 1141, 2077, 2105, 2787, 3044, 3134, 3159
Cac8I GCNNGC 5 cut(s) 144, 683, 2347, 2833, 3228
CaiI CAGNNNCTG 2 cut(s) 644, 1951
CciI TCATGA 3 cut(s) 1105, 1185, 2515
CfoI GCGC 2 cut(s) 1205, 3134
Cfr10I RCCGGY 1 cut(s) 1329
Cfr13I GGNCC 3 cut(s) 776, 1787, 3225
CseI GACGC 1 cut(s) 135
Csp6I GTAC 7 cut(s) 129, 226, 299, 592, 713, 1866, 2532
CspCI CAANNNNNGTGG 2 cut(s) 690, 725
CviQI GTAC 7 cut(s) 129, 226, 299, 592, 713, 1866, 2532
DraI TTTAAA 1 cut(s) 3028
DriI GACNNNNNGTC 1 cut(s) 3114
EaeI YGGCCR 2 cut(s) 834, 1140
Eam1104I CTCTTC 4 cut(s) 437, 2047, 2114, 2552
Eam1105I GACNNNNNGTC 1 cut(s) 3114
EarI CTCTTC 4 cut(s) 437, 2047, 2114, 2552
EciI GGCGGA 1 cut(s) 1241
Ecl136II GAGCTC 1 cut(s) 976
Eco130I CCWWGG 1 cut(s) 150
Eco24I GRGCYC 2 cut(s) 978, 985
Eco31I GGTCTC 1 cut(s) 914
Eco32I GATATC 1 cut(s) 1006
Eco53kI GAGCTC 1 cut(s) 976
Eco57I CTGAAG 8 cut(s) 62, 666, 954, 1140, 1584, 1626, 1693, 2160
EcoICRI GAGCTC 1 cut(s) 976
EcoRI GAATTC 4 cut(s) 936, 1267, 1738, 2582
EcoRII CCWGG 5 cut(s) 67, 289, 512, 1353, 2226
EcoRV GATATC 1 cut(s) 1006
EcoT14I CCWWGG 1 cut(s) 150
EcoT22I ATGCAT 1 cut(s) 1363
EcoT38I GRGCYC 2 cut(s) 978, 985
ErhI CCWWGG 1 cut(s) 150
Esp3I CGTCTC 1 cut(s) 1415
FalI AAGNNNNNCTT 4 cut(s) 711, 743, 2001, 2033
FaqI GGGAC 3 cut(s) 1594, 1610, 1958
FauI CCCGC 1 cut(s) 3235
FblI GTMKAC 1 cut(s) 15
Fnu4HI GCNGC 8 cut(s) 83, 448, 603, 639, 723, 2288, 2527, 3200
FriOI GRGCYC 2 cut(s) 978, 985
Fsp4HI GCNGC 8 cut(s) 83, 448, 603, 639, 723, 2288, 2527, 3200
FspAI RTGCGCAY 1 cut(s) 3133
FspI TGCGCA 1 cut(s) 3133
GlaI GCGC 2 cut(s) 1204, 3133
GluI GCNGC 8 cut(s) 83, 448, 603, 639, 723, 2288, 2527, 3200
GsaI CCCAGC 1 cut(s) 782
GsuI CTGGAG 4 cut(s) 1356, 1440, 1806, 3020
HaeII RGCGCY 1 cut(s) 1206
HaeIII GGCC 7 cut(s) 462, 777, 836, 1142, 1353, 1789, 3226
HapII CCGG 2 cut(s) 1330, 2291
HgaI GACGC 1 cut(s) 135
HhaI GCGC 2 cut(s) 1205, 3134
Hin6I GCGC 2 cut(s) 1203, 3132
HinP1I GCGC 2 cut(s) 1203, 3132
HincII GTYRAC 2 cut(s) 16, 1084
HindII GTYRAC 2 cut(s) 16, 1084
HindIII AAGCTT 6 cut(s) 578, 1112, 1260, 1906, 2306, 2698
HpaII CCGG 2 cut(s) 1330, 2291
HphI GGTGA 4 cut(s) 1430, 1823, 2534, 2909
Hpy166II GTNNAC 7 cut(s) 16, 1084, 1246, 1465, 1535, 2785, 3049
Hpy8I GTNNAC 7 cut(s) 16, 1084, 1246, 1465, 1535, 2785, 3049
HpyCH4IV ACGT 5 cut(s) 693, 1461, 2215, 2622, 3115
HpyF10VI GCNNNNNNNGC 6 cut(s) 82, 647, 1451, 1795, 2411, 3089
HpySE526I ACGT 5 cut(s) 693, 1461, 2215, 2622, 3115
HspAI GCGC 2 cut(s) 1203, 3132
KpnI GGTACC 2 cut(s) 132, 595
LguI GCTCTTC 1 cut(s) 437
LmnI GCTCC 5 cut(s) 455, 1326, 1459, 2908, 3080
Lsp1109I GCAGC 8 cut(s) 94, 434, 614, 650, 734, 2274, 2538, 3186
MaeII ACGT 5 cut(s) 693, 1461, 2215, 2622, 3115
MaeIII GTNAC 6 cut(s) 1301, 1825, 2211, 2540, 3070, 3116
MfeI CAATTG 1 cut(s) 98
MflI RGATCY 5 cut(s) 1045, 1606, 1985, 3246, 3255
MhlI GDGCHC 2 cut(s) 978, 985
MlsI TGGCCA 1 cut(s) 1142
MluNI TGGCCA 1 cut(s) 1142
MlyI GAGTC 1 cut(s) 2021
MmeI TCCRAC 1 cut(s) 1325
Mox20I TGGCCA 1 cut(s) 1142
Mph1103I ATGCAT 1 cut(s) 1363
MroXI GAANNNNTTC 2 cut(s) 1101, 1319
MscI TGGCCA 1 cut(s) 1142
MslI CAYNNNNRTG 4 cut(s) 425, 570, 2397, 3012
Msp20I TGGCCA 1 cut(s) 1142
MspA1I CMGCKG 2 cut(s) 605, 782
MspCI CTTAAG 2 cut(s) 122, 2546
MspI CCGG 2 cut(s) 1330, 2291
MspR9I CCNGG 5 cut(s) 69, 291, 514, 1355, 2228
MunI CAATTG 1 cut(s) 98
Mva1269I GAATGC 2 cut(s) 113, 1709
MvaI CCWGG 5 cut(s) 69, 291, 514, 1355, 2228
MwoI GCNNNNNNNGC 6 cut(s) 82, 647, 1451, 1795, 2411, 3089
NheI GCTAGC 1 cut(s) 142
NlaIV GGNNCC 5 cut(s) 130, 593, 1328, 2910, 3082
NmuCI GTSAC 4 cut(s) 1825, 2211, 2540, 3116
NsbI TGCGCA 1 cut(s) 3133
NsiI ATGCAT 1 cut(s) 1363
NspI RCATGY 2 cut(s) 894, 2349
NspV TTCGAA 2 cut(s) 1313, 1834
OliI CACNNNNGTG 2 cut(s) 425, 3012
PaeI GCATGC 1 cut(s) 2349
PagI TCATGA 3 cut(s) 1105, 1185, 2515
PasI CCCWGGG 1 cut(s) 513
PciSI GCTCTTC 1 cut(s) 437
PctI GAATGC 2 cut(s) 113, 1709
PdmI GAANNNNTTC 2 cut(s) 1101, 1319
PflMI CCANNNNNTGG 1 cut(s) 3055
PfoI TCCNGGA 1 cut(s) 289
PkrI GCNGC 8 cut(s) 84, 449, 604, 640, 724, 2289, 2528, 3201
PleI GAGTC 1 cut(s) 2020
PpsI GAGTC 1 cut(s) 2020
PshBI ATTAAT 1 cut(s) 1806
PsiI TTATAA 2 cut(s) 659, 2007
Psp124BI GAGCTC 1 cut(s) 978
Psp6I CCWGG 5 cut(s) 67, 289, 512, 1353, 2226
PspFI CCCAGC 1 cut(s) 778
PspGI CCWGG 5 cut(s) 67, 289, 512, 1353, 2226
PspN4I GGNNCC 5 cut(s) 130, 593, 1328, 2910, 3082
PspPI GGNCC 3 cut(s) 776, 1787, 3225
PstI CTGCAG 1 cut(s) 271
PstNI CAGNNNCTG 2 cut(s) 644, 1951
PsuI RGATCY 5 cut(s) 1045, 1606, 1985, 3246, 3255
PvuII CAGCTG 2 cut(s) 605, 782
RsaI GTAC 7 cut(s) 130, 227, 300, 593, 714, 1867, 2533
RsaNI GTAC 7 cut(s) 129, 226, 299, 592, 713, 1866, 2532
RseI CAYNNNNRTG 4 cut(s) 425, 570, 2397, 3012
SacI GAGCTC 1 cut(s) 978
SalI GTCGAC 1 cut(s) 14
SapI GCTCTTC 1 cut(s) 437
SatI GCNGC 8 cut(s) 83, 448, 603, 639, 723, 2288, 2527, 3200
Sau96I GGNCC 3 cut(s) 776, 1787, 3225
SchI GAGTC 1 cut(s) 2021
ScrFI CCNGG 5 cut(s) 69, 291, 514, 1355, 2228
SduI GDGCHC 2 cut(s) 978, 985
SfcI CTRYAG 3 cut(s) 267, 2750, 2980
SfuI TTCGAA 2 cut(s) 1313, 1834
SmiMI CAYNNNNRTG 4 cut(s) 425, 570, 2397, 3012
SmlI CTYRAG 4 cut(s) 122, 1569, 2546, 3121
SmoI CTYRAG 4 cut(s) 122, 1569, 2546, 3121
SpeI ACTAGT 1 cut(s) 179
SphI GCATGC 1 cut(s) 2349
SsiI CCGC 5 cut(s) 1252, 1730, 2926, 3228, 3267
SspI AATATT 2 cut(s) 2462, 2796
SstI GAGCTC 1 cut(s) 978
StyD4I CCNGG 5 cut(s) 67, 289, 512, 1353, 2226
StyI CCWWGG 1 cut(s) 150
TaiI ACGT 5 cut(s) 696, 1464, 2218, 2625, 3118
TaqI TCGA 6 cut(s) 15, 1313, 1834, 1896, 1941, 2878
TaqII GACCGA 1 cut(s) 1911
TatI WGTACW 1 cut(s) 298
TscAI CASTG 9 cut(s) 432, 714, 1141, 2077, 2112, 2787, 3044, 3134, 3159
TseFI GTSAC 4 cut(s) 1825, 2211, 2540, 3116
TseI GCWGC 8 cut(s) 82, 447, 602, 638, 722, 2287, 2526, 3199
Tsp45I GTSAC 4 cut(s) 1825, 2211, 2540, 3116
TspGWI ACGGA 7 cut(s) 394, 1069, 1609, 2210, 2979, 3024, 3195
TspRI CASTG 9 cut(s) 432, 714, 1141, 2077, 2112, 2787, 3044, 3134, 3159
Van91I CCANNNNNTGG 1 cut(s) 3055
Vha464I CTTAAG 2 cut(s) 122, 2546
VspI ATTAAT 1 cut(s) 1806
XceI RCATGY 2 cut(s) 894, 2349
XmiI GTMKAC 1 cut(s) 15
XmnI GAANNNNTTC 2 cut(s) 1101, 1319
Zsp2I ATGCAT 1 cut(s) 1363
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.