pycom03g03080

galactinol--sucrose galactosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0012127
Physical Location & Seq
Reverse (-)
2427712 .. 2430832
3121 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1665 bp
ATGACGGTTGGGGCTGGGATTTCTGTGGAGGATGGGAACTTGGTGGTGTTGGGGAACAAAGTGTTGTCTGAGGTTCATGACAACGTTGTGGTTACTCCGGCATCTGGGGGTGCATTGACCAATGGTGCTTTCATCGGTGTCCAATCTGATCAGGTTGGTAGCCGCAGGGTCTTCCCGATTGGCAAACTTGAGGGATTGCGCTTCATGTGCGTTTTCCGGTTTAAATTATGGTGGATGACACAGAGGATGGGCAATTGTGGCCAAGATGTTCCCTTTGAGACTCAGTTTTTGATTGTGGAAACAAAGGATGAGTCTCATTTTGGTGAAGGAAGCAGAGACGGGGCGGATCAGTCTGCAGCTTATACCGTTATCTTACCGATTCTCGAAGGGGACTTCAGGGCTGTTCTTCAAGGAAATGAACTTAATGAGATTGAAATCTGCCTAGAAAGTGGAGATCCTGCTGCTGATGGATTTGAGGGTAGTCATTTGGTTTTCGTTGGAGCGGGATCAGACCCATTCGATGTCATCGCAGATTCTGTGAAGACCGTGGAGAAACATTTGCAGACATTTTCTCATCGCGAGAGAAAGAAGATGCCAGACATGTTGAACTGGTTTGGCTGGTGTACGTGGGATGCTTTCTACACCGATGTGACTTCAGAAGGCCTGAAGCAAGGACTGCAGAGCCTGGAAAGTGGTGGAGCCCCTCCAAAGTTTGTTATTCTTGATGACGGATGGCAGTCAGTCAACATGGATTCCTCTGGTGTTGGATACGACGCTGATAATGCAGCAAACTTTGCAAACAGGTTAACACATATAAAAGAGAACCACAAATTTCAGAAAGATGGTAAAGAGGGTCACAGAGTAGAGGATCCTTCTTTGGGGCTTCGCCACATTGTTACCGAAATCAAAGAAAAACACGCTTTGAAATATGCTTATGTGTGGCATGCCATAACTGGATATTGGGGCGGTGTTAGACCTGGTGTTGCTGAAATGGAACACTATGATTCAAAGTTGGCTTACCCAATTTCATCTCCAGGAGTCGAGTCTAATGAGGATTGTGATGCCTTGAAAAGTATTACCACAAATGGGCTCGGCCTTGTGAATCCCGAGAAAGTTTTCAACTTTTATGATGAACTACACTCTTATCTCGCATCAGCTGGTATAGATGGAGTAAAGGTTGATGTTCAGAACATTCTTGAAACCCTAGGGGCAGGCCATGGTGGACGGGTAAAACTTACAAGAAAATACCATCAAGCATTGGAAGCGTCTGTCGCTAGAAACTTTCCTGACAATGGAATTATTTCTTGTATGAGTCACAATACAGATGGTTTATACAGTGTGAAGCGGACAGCTGTTATAAGGGCATCAGATGATTTCTGGCCTAGAGATCCAGCATCACACACAATTCACATCGCATCAGTTGCTTACAACACTGTTTTCCTTGGGGAGTTTATGCAGCCGGATTGGGATATGTTTCATAGCCTGCATCCAATGGCTGAATATCATGGAGCAGCTCGTGCGGTTGGAGGATGTGCAATTTATGTTAGTGACAAGCCTGGGCAGCATGACTTCGATATTCTGAGGAAGCTCGTACTTCCTGATGGATCGATCCAGCTTGAGGGCCAAACTTCCAGGAAGACCAACAAGGGATTGCTTATTGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 map00052
Pfam Domains
Protein Families
Raffinose_syn

Protein Analysis

555

Amino Acids

60.84

Weight (kDa)

5.37

Isoelectric Point (pI)

37.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raffinose_syn PF05691 7 - 544 4.9e-231 Raffinose synthase or seed imbibition protein Sip1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012127)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G55740
fragaria_vesca FvH4_3g41930 FvH4_3g41930 FvH4_3g41930
malus_domestica MD03G1040100.v1.1 MD11G1041500.v1.1
prunus_persica Prupe.6G032400_v2.0.a1 Prupe.6G032400_v2.0.a1
pyrus_communis pycom03g03080 pycom11g03510
rosa_chinensis RchiOBHm_Chr5g0075141
rosa_laevigata RLG00000036533
rosa_multiflora Rmu_ssc0000217.1_g000009
rosa_roxburghii Rroxscaffold_1G00006480
rosa_rugosa Rorug05G0436500
rosa_samantha Rh5AG493800 Rh5CG539100 Rh5DG527900
rosa_wichuraiana Rw5G045810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1358
AccBSI CCGCTC 1 cut(s) 503
AccII CGCG 1 cut(s) 579
AciI CCGC 6 cut(s) 163, 344, 503, 966, 1345, 1520
AclI AACGTT 1 cut(s) 84
AclWI GGATC 8 cut(s) 354, 449, 514, 863, 876, 1382, 1603, 1612
AcoI YGGCCR 1 cut(s) 259
AcsI RAATTY 1 cut(s) 830
AcuI CTGAAG 3 cut(s) 379, 639, 686
AfaI GTAC 2 cut(s) 625, 1593
AfiI CCNNNNNNNGG 5 cut(s) 104, 878, 1086, 1292, 1618
AflIII ACRYGT 1 cut(s) 600
AgsI TTSAA 8 cut(s) 410, 434, 607, 925, 1008, 1069, 1120, 1199
AjnI CCWGG 5 cut(s) 684, 976, 1033, 1555, 1631
AjuI GAANNNNNNNTTGG 2 cut(s) 113, 145
AleI CACNNNNGTG 1 cut(s) 647
AluBI AGCT 6 cut(s) 359, 1157, 1352, 1514, 1588, 1615
AluI AGCT 6 cut(s) 359, 1157, 1352, 1514, 1588, 1615
Alw26I GTCTC 3 cut(s) 272, 318, 330
AlwI GGATC 8 cut(s) 354, 449, 514, 863, 876, 1382, 1603, 1612
AlwNI CAGNNNCTG 2 cut(s) 536, 685
Ama87I CYCGRG 1 cut(s) 1106
AoxI GGCC 6 cut(s) 259, 661, 1093, 1213, 1379, 1621
ApeKI GCWGC 6 cut(s) 356, 461, 785, 1456, 1511, 1561
ApoI RAATTY 1 cut(s) 830
ArsI GACNNNNNNTTYG 4 cut(s) 271, 296, 303, 328
Asp700I GAANNNNTTC 2 cut(s) 1115, 1300
AspA2I CCTAGG 1 cut(s) 1204
AspLEI GCGC 1 cut(s) 201
AspS9I GGNCC 1 cut(s) 1621
AsuHPI GGTGA 1 cut(s) 335
AvaI CYCGRG 1 cut(s) 1106
AvrII CCTAGG 1 cut(s) 1204
BalI TGGCCA 1 cut(s) 261
BamHI GGATCC 1 cut(s) 868
BanII GRGCYC 2 cut(s) 703, 1092
BauI CACGAG 1 cut(s) 1515
BbsI GAAGAC 3 cut(s) 163, 548, 1643
BbvI GCAGC 6 cut(s) 368, 448, 797, 1468, 1523, 1573
BccI CCATC 9 cut(s) 26, 241, 461, 726, 836, 1160, 1257, 1319, 1595
BciT130I CCWGG 5 cut(s) 686, 978, 1035, 1557, 1633
BciVI GTATCC 1 cut(s) 761
BclI TGATCA 1 cut(s) 148
BcoDI GTCTC 3 cut(s) 272, 318, 330
BfaI CTAG 4 cut(s) 443, 1205, 1275, 1383
BfmI CTRYAG 2 cut(s) 354, 677
BfuI GTATCC 1 cut(s) 761
BisI GCNGC 7 cut(s) 163, 357, 462, 786, 1457, 1512, 1562
BlnI CCTAGG 1 cut(s) 1204
BlsI GCNGC 7 cut(s) 164, 358, 463, 787, 1458, 1513, 1563
Bme1390I CCNGG 5 cut(s) 686, 978, 1035, 1557, 1633
BmeT110I CYCGRG 1 cut(s) 1106
BmgT120I GGNCC 1 cut(s) 1621
BmiI GGNNCC 2 cut(s) 700, 870
BmrFI CCNGG 5 cut(s) 686, 978, 1035, 1557, 1633
BmsI GCATC 9 cut(s) 110, 582, 622, 1051, 1160, 1373, 1403, 1424, 1495
BpiI GAAGAC 3 cut(s) 163, 548, 1643
BpmI CTGGAG 1 cut(s) 1017
BpuEI CTTGAG 2 cut(s) 209, 1637
Bsa29I ATCGAT 1 cut(s) 1607
BsaAI YACGTR 1 cut(s) 627
BsaBI GATNNNNATC 1 cut(s) 434
BsaJI CCNNGG 5 cut(s) 546, 1204, 1216, 1441, 1556
BsaWI WCCGGW 1 cut(s) 216
BsaXI ACNNNNNCTCC 2 cut(s) 1161, 1191
Bsc4I CCNNNNNNNGG 5 cut(s) 104, 878, 1086, 1292, 1618
Bse1I ACTGG 2 cut(s) 614, 958
Bse8I GATNNNNATC 1 cut(s) 434
BseBI CCWGG 5 cut(s) 686, 978, 1035, 1557, 1633
BseCI ATCGAT 1 cut(s) 1607
BseDI CCNNGG 5 cut(s) 546, 1204, 1216, 1441, 1556
BseGI GGATG 8 cut(s) 37, 240, 252, 313, 637, 737, 1486, 1535
BseJI GATNNNNATC 1 cut(s) 434
BseLI CCNNNNNNNGG 5 cut(s) 104, 878, 1086, 1292, 1618
BseMII CTCAG 3 cut(s) 60, 296, 1571
BseNI ACTGG 2 cut(s) 614, 958
BseXI GCAGC 6 cut(s) 368, 448, 797, 1468, 1523, 1573
BseYI CCCAGC 1 cut(s) 14
Bsh1236I CGCG 1 cut(s) 579
BshFI GGCC 6 cut(s) 261, 663, 1095, 1215, 1381, 1623
BshVI ATCGAT 1 cut(s) 1607
BsiHKCI CYCGRG 1 cut(s) 1106
BsiSI CCGG 3 cut(s) 98, 217, 1460
BslFI GGGAC 1 cut(s) 404
BslI CCNNNNNNNGG 5 cut(s) 104, 878, 1086, 1292, 1618
BsmAI GTCTC 3 cut(s) 272, 318, 330
BsmBI CGTCTC 1 cut(s) 330
BsmFI GGGAC 1 cut(s) 404
BsnI GGCC 6 cut(s) 261, 663, 1095, 1215, 1381, 1623
BsoBI CYCGRG 1 cut(s) 1106
Bsp1286I GDGCHC 2 cut(s) 703, 1092
Bsp143I GATC 8 cut(s) 148, 346, 454, 506, 868, 1387, 1604, 1608
Bsp19I CCATGG 1 cut(s) 1216
Bsp68I TCGCGA 1 cut(s) 579
BspACI CCGC 6 cut(s) 163, 344, 503, 966, 1345, 1520
BspANI GGCC 6 cut(s) 261, 663, 1095, 1215, 1381, 1623
BspCNI CTCAG 3 cut(s) 61, 295, 1572
BspDI ATCGAT 1 cut(s) 1607
BspFNI CGCG 1 cut(s) 579
BspHI TCATGA 1 cut(s) 76
BspLI GGNNCC 2 cut(s) 700, 870
BspMAI CTGCAG 2 cut(s) 358, 681
BspPI GGATC 8 cut(s) 354, 449, 514, 863, 876, 1382, 1603, 1612
BsrBI CCGCTC 1 cut(s) 503
BsrI ACTGG 2 cut(s) 614, 958
BssECI CCNNGG 5 cut(s) 546, 1204, 1216, 1441, 1556
BssMI GATC 8 cut(s) 148, 346, 454, 506, 868, 1387, 1604, 1608
BssSI CACGAG 1 cut(s) 1515
BssT1I CCWWGG 3 cut(s) 1204, 1216, 1441
Bst2BI CACGAG 1 cut(s) 1515
Bst2UI CCWGG 5 cut(s) 686, 978, 1035, 1557, 1633
Bst4CI ACNGT 5 cut(s) 7, 367, 547, 1337, 1435
BstAPI GCANNNNNTGC 4 cut(s) 676, 794, 1421, 1517
BstBAI YACGTR 1 cut(s) 627
BstC8I GCNNGC 3 cut(s) 945, 1213, 1484
BstDEI CTNAG 3 cut(s) 69, 282, 1580
BstDSI CCRYGG 2 cut(s) 546, 1216
BstF5I GGATG 8 cut(s) 37, 240, 252, 313, 637, 737, 1486, 1535
BstFNI CGCG 1 cut(s) 579
BstHHI GCGC 1 cut(s) 201
BstKTI GATC 8 cut(s) 151, 349, 457, 509, 871, 1390, 1607, 1611
BstMAI GTCTC 3 cut(s) 272, 318, 330
BstMBI GATC 8 cut(s) 148, 346, 454, 506, 868, 1387, 1604, 1608
BstNI CCWGG 5 cut(s) 686, 978, 1035, 1557, 1633
BstNSI RCATGY 2 cut(s) 604, 947
BstSCI CCNGG 5 cut(s) 684, 976, 1033, 1555, 1631
BstSFI CTRYAG 2 cut(s) 354, 677
BstUI CGCG 1 cut(s) 579
BstV1I GCAGC 6 cut(s) 368, 448, 797, 1468, 1523, 1573
BstV2I GAAGAC 3 cut(s) 163, 548, 1643
BstX2I RGATCY 3 cut(s) 454, 868, 1387
BstYI RGATCY 3 cut(s) 454, 868, 1387
Bsu15I ATCGAT 1 cut(s) 1607
BsuI GTATCC 1 cut(s) 761
BsuRI GGCC 6 cut(s) 261, 663, 1095, 1215, 1381, 1623
BsuTUI ATCGAT 1 cut(s) 1607
BtgI CCRYGG 2 cut(s) 546, 1216
BtgZI GCGATG 3 cut(s) 511, 560, 1396
BtsCI GGATG 8 cut(s) 37, 240, 252, 313, 637, 737, 1486, 1535
BtsIMutI CAGTG 2 cut(s) 1342, 1431
BtuMI TCGCGA 1 cut(s) 579
Cac8I GCNNGC 3 cut(s) 945, 1213, 1484
CaiI CAGNNNCTG 2 cut(s) 536, 685
CciI TCATGA 1 cut(s) 76
CfoI GCGC 1 cut(s) 201
Cfr13I GGNCC 1 cut(s) 1621
ClaI ATCGAT 1 cut(s) 1607
CseI GACGC 2 cut(s) 782, 1254
CsiI ACCWGGT 1 cut(s) 976
Csp6I GTAC 2 cut(s) 624, 1592
CviAII CATG 8 cut(s) 77, 205, 601, 748, 944, 1217, 1505, 1565
CviQI GTAC 2 cut(s) 624, 1592
DdeI CTNAG 3 cut(s) 69, 282, 1580
DpnI GATC 8 cut(s) 150, 348, 456, 508, 870, 1389, 1606, 1610
DpnII GATC 8 cut(s) 148, 346, 454, 506, 868, 1387, 1604, 1608
DraI TTTAAA 1 cut(s) 223
EaeI YGGCCR 1 cut(s) 259
EciI GGCGGA 1 cut(s) 359
Eco130I CCWWGG 3 cut(s) 1204, 1216, 1441
Eco147I AGGCCT 1 cut(s) 663
Eco24I GRGCYC 2 cut(s) 703, 1092
Eco57I CTGAAG 3 cut(s) 379, 639, 686
Eco88I CYCGRG 1 cut(s) 1106
EcoRII CCWGG 5 cut(s) 684, 976, 1033, 1555, 1631
EcoT14I CCWWGG 3 cut(s) 1204, 1216, 1441
EcoT38I GRGCYC 2 cut(s) 703, 1092
ErhI CCWWGG 3 cut(s) 1204, 1216, 1441
Esp3I CGTCTC 1 cut(s) 330
FaeI CATG 8 cut(s) 80, 208, 604, 751, 947, 1220, 1508, 1568
FaqI GGGAC 1 cut(s) 404
FatI CATG 8 cut(s) 76, 204, 600, 747, 943, 1216, 1504, 1564
FauI CCCGC 1 cut(s) 496
FbaI TGATCA 1 cut(s) 148
Fnu4HI GCNGC 7 cut(s) 163, 357, 462, 786, 1457, 1512, 1562
FokI GGATG 8 cut(s) 44, 247, 259, 320, 644, 744, 1473, 1542
FriOI GRGCYC 2 cut(s) 703, 1092
Fsp4HI GCNGC 7 cut(s) 163, 357, 462, 786, 1457, 1512, 1562
FspBI CTAG 4 cut(s) 443, 1205, 1275, 1383
GlaI GCGC 1 cut(s) 200
GluI GCNGC 7 cut(s) 163, 357, 462, 786, 1457, 1512, 1562
GsaI CCCAGC 1 cut(s) 18
GsuI CTGGAG 1 cut(s) 1017
HaeIII GGCC 6 cut(s) 261, 663, 1095, 1215, 1381, 1623
HapII CCGG 3 cut(s) 98, 217, 1460
HgaI GACGC 2 cut(s) 782, 1254
HhaI GCGC 1 cut(s) 201
Hin1II CATG 8 cut(s) 80, 208, 604, 751, 947, 1220, 1508, 1568
Hin6I GCGC 1 cut(s) 199
HinP1I GCGC 1 cut(s) 199
HincII GTYRAC 2 cut(s) 745, 807
HindII GTYRAC 2 cut(s) 745, 807
HpaI GTTAAC 1 cut(s) 807
HpaII CCGG 3 cut(s) 98, 217, 1460
HphI GGTGA 1 cut(s) 335
Hpy166II GTNNAC 4 cut(s) 624, 745, 807, 1223
Hpy188I TCNGA 9 cut(s) 70, 148, 511, 658, 837, 1188, 1369, 1581, 1664
Hpy188III TCNNGA 9 cut(s) 77, 175, 383, 578, 722, 1106, 1196, 1286, 1598
Hpy8I GTNNAC 4 cut(s) 624, 745, 807, 1223
Hpy99I CGWCG 1 cut(s) 776
HpyAV CCTTC 4 cut(s) 320, 380, 653, 882
HpyCH4III ACNGT 5 cut(s) 7, 367, 547, 1337, 1435
HpyCH4IV ACGT 2 cut(s) 84, 626
HpyCH4V TGCA 9 cut(s) 113, 356, 562, 679, 785, 797, 1456, 1486, 1535
HpyF3I CTNAG 3 cut(s) 69, 282, 1580
HpySE526I ACGT 2 cut(s) 84, 626
Hsp92II CATG 8 cut(s) 80, 208, 604, 751, 947, 1220, 1508, 1568
HspAI GCGC 1 cut(s) 199
Ksp22I TGATCA 1 cut(s) 148
KspAI GTTAAC 1 cut(s) 807
Kzo9I GATC 8 cut(s) 148, 346, 454, 506, 868, 1387, 1604, 1608
LmnI GCTCC 3 cut(s) 500, 698, 1508
Lsp1109I GCAGC 6 cut(s) 368, 448, 797, 1468, 1523, 1573
LweI GCATC 9 cut(s) 110, 582, 622, 1051, 1160, 1373, 1403, 1424, 1495
MabI ACCWGGT 1 cut(s) 976
MaeI CTAG 4 cut(s) 443, 1205, 1275, 1383
MaeII ACGT 2 cut(s) 84, 626
MaeIII GTNAC 6 cut(s) 91, 649, 854, 895, 1313, 1547
MalI GATC 8 cut(s) 150, 348, 456, 508, 870, 1389, 1606, 1610
MbiI CCGCTC 1 cut(s) 503
MboI GATC 8 cut(s) 148, 346, 454, 506, 868, 1387, 1604, 1608
MboII GAAGA 5 cut(s) 163, 398, 553, 601, 1648
MfeI CAATTG 1 cut(s) 253
MflI RGATCY 3 cut(s) 454, 868, 1387
MhlI GDGCHC 2 cut(s) 703, 1092
MlsI TGGCCA 1 cut(s) 261
MluCI AATT 7 cut(s) 224, 253, 830, 1023, 1296, 1404, 1536
MluNI TGGCCA 1 cut(s) 261
MlyI GAGTC 5 cut(s) 274, 320, 1047, 1052, 1321
MmeI TCCRAC 3 cut(s) 478, 745, 1504
Mox20I TGGCCA 1 cut(s) 261
MroXI GAANNNNTTC 2 cut(s) 1115, 1300
MscI TGGCCA 1 cut(s) 261
MseI TTAA 3 cut(s) 222, 423, 806
MslI CAYNNNNRTG 2 cut(s) 321, 647
Msp20I TGGCCA 1 cut(s) 261
MspA1I CMGCKG 2 cut(s) 1157, 1352
MspI CCGG 3 cut(s) 98, 217, 1460
MspR9I CCNGG 5 cut(s) 686, 978, 1035, 1557, 1633
MunI CAATTG 1 cut(s) 253
MvaI CCWGG 5 cut(s) 686, 978, 1035, 1557, 1633
MvnI CGCG 1 cut(s) 579
NcoI CCATGG 1 cut(s) 1216
NdeII GATC 8 cut(s) 148, 346, 454, 506, 868, 1387, 1604, 1608
NlaIII CATG 8 cut(s) 80, 208, 604, 751, 947, 1220, 1508, 1568
NlaIV GGNNCC 2 cut(s) 700, 870
NmeAIII GCCGAG 1 cut(s) 1071
NmuCI GTSAC 4 cut(s) 649, 854, 1313, 1547
NruI TCGCGA 1 cut(s) 579
NspI RCATGY 2 cut(s) 604, 947
OliI CACNNNNGTG 1 cut(s) 647
PaeI GCATGC 1 cut(s) 947
PagI TCATGA 1 cut(s) 76
PceI AGGCCT 1 cut(s) 663
PciI ACATGT 1 cut(s) 600
PdmI GAANNNNTTC 2 cut(s) 1115, 1300
PfeI GAWTC 5 cut(s) 379, 533, 752, 1004, 1102
PfoI TCCNGGA 2 cut(s) 1033, 1631
PkrI GCNGC 7 cut(s) 164, 358, 463, 787, 1458, 1513, 1563
PleI GAGTC 5 cut(s) 274, 319, 1046, 1051, 1320
PpsI GAGTC 5 cut(s) 274, 319, 1046, 1051, 1320
Ppu21I YACGTR 1 cut(s) 627
PscI ACATGT 1 cut(s) 600
PsiI TTATAA 1 cut(s) 1358
Psp1406I AACGTT 1 cut(s) 84
Psp6I CCWGG 5 cut(s) 684, 976, 1033, 1555, 1631
PspFI CCCAGC 1 cut(s) 14
PspGI CCWGG 5 cut(s) 684, 976, 1033, 1555, 1631
PspN4I GGNNCC 2 cut(s) 700, 870
PspPI GGNCC 1 cut(s) 1621
PstI CTGCAG 2 cut(s) 358, 681
PstNI CAGNNNCTG 2 cut(s) 536, 685
PsuI RGATCY 3 cut(s) 454, 868, 1387
PvuII CAGCTG 2 cut(s) 1157, 1352
RruI TCGCGA 1 cut(s) 579
RsaI GTAC 2 cut(s) 625, 1593
RsaNI GTAC 2 cut(s) 624, 1592
RseI CAYNNNNRTG 2 cut(s) 321, 647
SaqAI TTAA 3 cut(s) 222, 423, 806
SatI GCNGC 7 cut(s) 163, 357, 462, 786, 1457, 1512, 1562
Sau3AI GATC 8 cut(s) 148, 346, 454, 506, 868, 1387, 1604, 1608
Sau96I GGNCC 1 cut(s) 1621
SchI GAGTC 5 cut(s) 274, 320, 1047, 1052, 1321
ScrFI CCNGG 5 cut(s) 686, 978, 1035, 1557, 1633
SduI GDGCHC 2 cut(s) 703, 1092
SexAI ACCWGGT 1 cut(s) 976
SfaNI GCATC 9 cut(s) 110, 582, 622, 1051, 1160, 1373, 1403, 1424, 1495
SfcI CTRYAG 2 cut(s) 354, 677
SmiMI CAYNNNNRTG 2 cut(s) 321, 647
SmlI CTYRAG 2 cut(s) 188, 1616
SmoI CTYRAG 2 cut(s) 188, 1616
SphI GCATGC 1 cut(s) 947
Sse9I AATT 7 cut(s) 224, 253, 830, 1023, 1296, 1404, 1536
SseBI AGGCCT 1 cut(s) 663
SsiI CCGC 6 cut(s) 163, 344, 503, 966, 1345, 1520
SspMI CTAG 4 cut(s) 443, 1205, 1275, 1383
StuI AGGCCT 1 cut(s) 663
StyD4I CCNGG 5 cut(s) 684, 976, 1033, 1555, 1631
StyI CCWWGG 3 cut(s) 1204, 1216, 1441
TaaI ACNGT 5 cut(s) 7, 367, 547, 1337, 1435
TaiI ACGT 2 cut(s) 87, 629
TaqI TCGA 5 cut(s) 384, 519, 1041, 1572, 1607
TasI AATT 7 cut(s) 224, 253, 830, 1023, 1296, 1404, 1536
TauI GCSGC 1 cut(s) 165
TfiI GAWTC 5 cut(s) 379, 533, 752, 1004, 1102
Tru1I TTAA 3 cut(s) 222, 423, 806
Tru9I TTAA 3 cut(s) 222, 423, 806
TscAI CASTG 2 cut(s) 1342, 1438
TseFI GTSAC 4 cut(s) 649, 854, 1313, 1547
TseI GCWGC 6 cut(s) 356, 461, 785, 1456, 1511, 1561
Tsp45I GTSAC 4 cut(s) 649, 854, 1313, 1547
TspDTI ATGAA 7 cut(s) 65, 121, 193, 432, 1017, 1146, 1466
TspGWI ACGGA 1 cut(s) 744
TspRI CASTG 2 cut(s) 1342, 1438
XapI RAATTY 1 cut(s) 830
XceI RCATGY 2 cut(s) 604, 947
XmaJI CCTAGG 1 cut(s) 1204
XmnI GAANNNNTTC 2 cut(s) 1115, 1300
XspI CTAG 4 cut(s) 443, 1205, 1275, 1383
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.