pycom03g12540

NAD(P)H-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0014247
Physical Location & Seq
Reverse (-)
13141986 .. 13146752
4767 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1161 bp
ATGAAAAATATCACTTACTATAAATGGAATGGATATTTGGGGGACATTTTGGTAAATCTGATAAGAAATACAACGACCACAAGTCATAAACTGCTGTTTCCATCTCCTCTTTCCCTCCTCTGCTTTCTTGCTCGCATGGCCATGGCTTCCAGACTCTCACCTACTTCGATTCTTAATCTTCCAATCCCAGCTAGAAATCTCCCAAACCACCCTCACAGAATACCTCTTTTTTCTATTCCACCACCTCTGGGTCTCCATGCAAATTCCTCCTTCAATTCAACAACCATTTCGCGCTCTAAAGGACGATCATCGCCACCGGCACTTGGTGCTGTCCAAGAGGAAGTCGCCCAGTCCCCAAGTTCAGAGCGCACCGGAAATTCAGAAGCTTCCCCTTCTTCCTCTTCCAAGCTCGTTCTCGTCATTGGCGGCACCGGCGGCGTTGGTCAGCTGGTGGTGGCTTCGTTGCTCGACCGGAATATCAAGTCCCGACTATTGTTGCGTGACCCCGAGAAAGCCACTACATTATTTGGTGAACACGATGAAGAAACATTGCAGGTATTCAAAGGGGACACTCGGAATCCAGAGGATCTGGATCCATCTATTTTTGAGGGAGTCACCCATGTGATTTGCTGCTCGGGAACTACTGCCTTTCCTTCAAAGCGGTGGGAAGGCGATAATACACCAGAAAGAGTTGATTGGGAGGGTGTGAGAAATCTTATATCTGCACTGCCTTCATCGCTGAAGAGAATTGTTCTTGTATCATCTGTAGGCGTAACCAAGTCAAATGAACTGCCATGGAGCATTATGAACCTTTTTGGCGTTCTCAAATATAAAAAGATGGGGGAGGATATGCTTCGTAATTCTGGCCTACCATTTACAATAATCAGACCTGGTAGATTGACAGATGGACCTTACACATCATATGATCTTAATACATTGCTCAAAGCTACAGCTGGCAAACGGCGTGCAGTCCTATTAGGTCAAGGAGATAAGCTAGTTGGAGAGGTTAGCAGGCATGTCGTTGCTGAAGCTTGCATACAGGCGTTGGACATAGAATGTACTGAAGGACAAATTTTTGAAGTCAACTCGGTCGAGGGAGAAGGCCCAGGAAGTGATCAGCAAAAGTGGCGGGAATTGTTCAAAGCTGCCCGAGCTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAD_binding_10

Protein Analysis

387

Amino Acids

42.1

Weight (kDa)

8.89

Isoelectric Point (pI)

52.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 135 - 213 8.1e-08 NmrA-like family
Epimerase PF01370 138 - 259 1.2e-07 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 139 - 270 1.2e-06 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_10 PF13460 142 - 350 1e-29 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014247)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G31530 AT4G31530
fragaria_vesca FvH4_3g28150
malus_domestica MD03G1169200.v1.1
prunus_persica Prupe.4G258400_v2.0.a1 Prupe.4G258400_v2.0.a1
pyrus_communis pycom03g12540
rosa_chinensis RchiOBHm_Chr5g0050831
rosa_laevigata RLG00000034742
rosa_multiflora Rmu_sc0007394.1_g000008
rosa_roxburghii Rroxscaffold_1G00029880
rosa_rugosa Rorug05G0264500.1
rosa_samantha Rh5AG336500 Rh5BG346000 Rh5CG373700 Rh5DG359000
rosa_wichuraiana Rw5G031730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 544
AccB1I GGYRCC 1 cut(s) 428
AccII CGCG 1 cut(s) 292
AciI CCGC 4 cut(s) 426, 435, 661, 1129
AclWI GGATC 3 cut(s) 587, 594, 600
AcoI YGGCCR 1 cut(s) 138
AcsI RAATTY 3 cut(s) 262, 376, 1071
AcuI CTGAAG 3 cut(s) 761, 1047, 1083
AdeI CACNNNGTG 1 cut(s) 326
AfaI GTAC 1 cut(s) 1060
AfiI CCNNNNNNNGG 2 cut(s) 248, 323
AgsI TTSAA 6 cut(s) 274, 279, 562, 657, 1079, 1141
AhdI GACNNNNNGTC 1 cut(s) 81
AjnI CCWGG 2 cut(s) 889, 1105
AjuI GAANNNNNNNTTGG 2 cut(s) 20, 52
AleI CACNNNNGTG 1 cut(s) 620
Alw21I GWGCWC 1 cut(s) 1156
Alw26I GTCTC 1 cut(s) 257
AlwI GGATC 3 cut(s) 587, 594, 600
Ama87I CYCGRG 3 cut(s) 506, 634, 1149
AoxI GGCC 3 cut(s) 138, 865, 1102
ApeKI GCWGC 2 cut(s) 630, 1145
ApoI RAATTY 3 cut(s) 262, 376, 1071
AspLEI GCGC 2 cut(s) 294, 369
AspS9I GGNCC 2 cut(s) 908, 1103
AsuHPI GGTGA 3 cut(s) 150, 542, 607
AvaI CYCGRG 3 cut(s) 506, 634, 1149
AvaII GGWCC 1 cut(s) 908
BalI TGGCCA 1 cut(s) 140
BamHI GGATCC 1 cut(s) 592
BanI GGYRCC 1 cut(s) 428
BanII GRGCYC 1 cut(s) 1156
BarI GAAGNNNNNNTAC 2 cut(s) 1020, 1052
Bbv12I GWGCWC 1 cut(s) 1156
BbvI GCAGC 2 cut(s) 617, 1132
BccI CCATC 4 cut(s) 109, 604, 832, 899
BceAI ACGGC 1 cut(s) 977
BcgI CGANNNNNNTGC 2 cut(s) 1000, 1034
BciT130I CCWGG 2 cut(s) 891, 1107
BclI TGATCA 1 cut(s) 1114
BcoDI GTCTC 1 cut(s) 257
BfaI CTAG 2 cut(s) 192, 995
BfmI CTRYAG 2 cut(s) 765, 948
BfuAI ACCTGC 1 cut(s) 544
BisI GCNGC 4 cut(s) 427, 436, 631, 1146
BlsI GCNGC 4 cut(s) 428, 437, 632, 1147
Bme1390I CCNGG 2 cut(s) 891, 1107
Bme18I GGWCC 1 cut(s) 908
BmeRI GACNNNNNGTC 1 cut(s) 81
BmeT110I CYCGRG 3 cut(s) 506, 634, 1149
BmgT120I GGNCC 2 cut(s) 908, 1103
BmiI GGNNCC 2 cut(s) 430, 594
BmrFI CCNGG 2 cut(s) 891, 1107
BmrI ACTGGG 1 cut(s) 343
BmuI ACTGGG 1 cut(s) 343
BsaBI GATNNNNATC 1 cut(s) 591
BsaI GGTCTC 1 cut(s) 257
BsaJI CCNNGG 3 cut(s) 141, 794, 1105
BsaWI WCCGGW 2 cut(s) 371, 471
Bsc4I CCNNNNNNNGG 2 cut(s) 248, 323
Bse118I RCCGGY 2 cut(s) 316, 431
Bse1I ACTGG 1 cut(s) 349
Bse3DI GCAATG 2 cut(s) 548, 935
Bse8I GATNNNNATC 1 cut(s) 591
BseBI CCWGG 2 cut(s) 891, 1107
BseDI CCNNGG 3 cut(s) 141, 794, 1105
BseJI GATNNNNATC 1 cut(s) 591
BseLI CCNNNNNNNGG 2 cut(s) 248, 323
BseMI GCAATG 2 cut(s) 548, 935
BseNI ACTGG 1 cut(s) 349
BseRI GAGGAG 2 cut(s) 96, 107
BseXI GCAGC 2 cut(s) 617, 1132
BseYI CCCAGC 1 cut(s) 187
BsgI GTGCAG 2 cut(s) 708, 987
Bsh1236I CGCG 1 cut(s) 292
Bsh1285I CGRYCG 2 cut(s) 472, 1092
BshFI GGCC 3 cut(s) 140, 867, 1104
BshNI GGYRCC 1 cut(s) 428
BsiEI CGRYCG 2 cut(s) 472, 1092
BsiHKAI GWGCWC 1 cut(s) 1156
BsiHKCI CYCGRG 3 cut(s) 506, 634, 1149
BsiSI CCGG 4 cut(s) 317, 372, 432, 472
BslFI GGGAC 4 cut(s) 56, 337, 469, 581
BslI CCNNNNNNNGG 2 cut(s) 248, 323
BsmAI GTCTC 1 cut(s) 257
BsmFI GGGAC 4 cut(s) 56, 337, 469, 581
BsnI GGCC 3 cut(s) 140, 867, 1104
Bso31I GGTCTC 1 cut(s) 257
BsoBI CYCGRG 3 cut(s) 506, 634, 1149
Bsp1286I GDGCHC 1 cut(s) 1156
Bsp143I GATC 5 cut(s) 305, 586, 592, 925, 1114
Bsp19I CCATGG 2 cut(s) 141, 794
BspACI CCGC 4 cut(s) 426, 435, 661, 1129
BspANI GGCC 3 cut(s) 140, 867, 1104
BspFNI CGCG 1 cut(s) 292
BspLI GGNNCC 2 cut(s) 430, 594
BspMI ACCTGC 1 cut(s) 544
BspPI GGATC 3 cut(s) 587, 594, 600
BspT107I GGYRCC 1 cut(s) 428
BspTNI GGTCTC 1 cut(s) 257
BsrDI GCAATG 2 cut(s) 548, 935
BsrFI RCCGGY 2 cut(s) 316, 431
BsrI ACTGG 1 cut(s) 349
BssAI RCCGGY 2 cut(s) 316, 431
BssECI CCNNGG 3 cut(s) 141, 794, 1105
BssMI GATC 5 cut(s) 305, 586, 592, 925, 1114
BssT1I CCWWGG 2 cut(s) 141, 794
Bst2UI CCWGG 2 cut(s) 891, 1107
Bst6I CTCTTC 2 cut(s) 406, 737
BstAPI GCANNNNNTGC 1 cut(s) 326
BstC8I GCNNGC 5 cut(s) 133, 955, 966, 1013, 1033
BstDSI CCRYGG 2 cut(s) 141, 794
BstFNI CGCG 1 cut(s) 292
BstHHI GCGC 2 cut(s) 294, 369
BstKTI GATC 5 cut(s) 308, 589, 595, 928, 1117
BstMAI GTCTC 1 cut(s) 257
BstMBI GATC 5 cut(s) 305, 586, 592, 925, 1114
BstMCI CGRYCG 2 cut(s) 472, 1092
BstMWI GCNNNNNNNGC 7 cut(s) 137, 326, 432, 435, 736, 1126, 1151
BstNI CCWGG 2 cut(s) 891, 1107
BstNSI RCATGY 1 cut(s) 1019
BstSCI CCNGG 2 cut(s) 889, 1105
BstSFI CTRYAG 2 cut(s) 765, 948
BstUI CGCG 1 cut(s) 292
BstV1I GCAGC 2 cut(s) 617, 1132
BstX2I RGATCY 2 cut(s) 586, 592
BstYI RGATCY 2 cut(s) 586, 592
BsuRI GGCC 3 cut(s) 140, 867, 1104
BtgI CCRYGG 2 cut(s) 141, 794
BtgZI GCGATG 2 cut(s) 294, 720
BtsI GCAGTG 1 cut(s) 725
BtsIMutI CAGTG 1 cut(s) 725
BveI ACCTGC 1 cut(s) 544
Cac8I GCNNGC 5 cut(s) 133, 955, 966, 1013, 1033
CfoI GCGC 2 cut(s) 294, 369
Cfr10I RCCGGY 2 cut(s) 316, 431
Cfr13I GGNCC 2 cut(s) 908, 1103
CsiI ACCWGGT 1 cut(s) 889
Csp6I GTAC 1 cut(s) 1059
CviAII CATG 6 cut(s) 136, 142, 257, 620, 795, 1016
CviQI GTAC 1 cut(s) 1059
DpnI GATC 5 cut(s) 307, 588, 594, 927, 1116
DpnII GATC 5 cut(s) 305, 586, 592, 925, 1114
DraIII CACNNNGTG 1 cut(s) 326
DriI GACNNNNNGTC 1 cut(s) 81
EaeI YGGCCR 1 cut(s) 138
Eam1104I CTCTTC 2 cut(s) 406, 737
Eam1105I GACNNNNNGTC 1 cut(s) 81
EarI CTCTTC 2 cut(s) 406, 737
Ecl136II GAGCTC 1 cut(s) 1154
Eco130I CCWWGG 2 cut(s) 141, 794
Eco24I GRGCYC 1 cut(s) 1156
Eco31I GGTCTC 1 cut(s) 257
Eco47I GGWCC 1 cut(s) 908
Eco53kI GAGCTC 1 cut(s) 1154
Eco57I CTGAAG 3 cut(s) 761, 1047, 1083
Eco88I CYCGRG 3 cut(s) 506, 634, 1149
EcoICRI GAGCTC 1 cut(s) 1154
EcoRII CCWGG 2 cut(s) 889, 1105
EcoT14I CCWWGG 2 cut(s) 141, 794
EcoT38I GRGCYC 1 cut(s) 1156
ErhI CCWWGG 2 cut(s) 141, 794
FaeI CATG 6 cut(s) 139, 145, 260, 623, 798, 1019
FaqI GGGAC 4 cut(s) 56, 337, 469, 581
FatI CATG 6 cut(s) 135, 141, 256, 619, 794, 1015
FauI CCCGC 1 cut(s) 1122
FauNDI CATATG 1 cut(s) 922
FbaI TGATCA 1 cut(s) 1114
Fnu4HI GCNGC 4 cut(s) 427, 436, 631, 1146
FriOI GRGCYC 1 cut(s) 1156
Fsp4HI GCNGC 4 cut(s) 427, 436, 631, 1146
FspBI CTAG 2 cut(s) 192, 995
GlaI GCGC 2 cut(s) 293, 368
GluI GCNGC 4 cut(s) 427, 436, 631, 1146
GsaI CCCAGC 1 cut(s) 191
HaeIII GGCC 3 cut(s) 140, 867, 1104
HapII CCGG 4 cut(s) 317, 372, 432, 472
HhaI GCGC 2 cut(s) 294, 369
Hin1II CATG 6 cut(s) 139, 145, 260, 623, 798, 1019
Hin6I GCGC 2 cut(s) 292, 367
HinP1I GCGC 2 cut(s) 292, 367
HincII GTYRAC 1 cut(s) 1084
HindII GTYRAC 1 cut(s) 1084
HindIII AAGCTT 2 cut(s) 384, 1029
HinfI GANTC 4 cut(s) 153, 169, 577, 612
HpaII CCGG 4 cut(s) 317, 372, 432, 472
HphI GGTGA 3 cut(s) 150, 542, 607
Hpy166II GTNNAC 2 cut(s) 533, 1084
Hpy188I TCNGA 5 cut(s) 60, 364, 382, 576, 887
Hpy188III TCNNGA 5 cut(s) 150, 486, 581, 590, 636
Hpy8I GTNNAC 2 cut(s) 533, 1084
HpyAV CCTTC 7 cut(s) 280, 402, 662, 663, 741, 1058, 1094
HpyCH4V TGCA 5 cut(s) 260, 553, 725, 968, 1035
HpyF10VI GCNNNNNNNGC 7 cut(s) 137, 326, 432, 435, 736, 1126, 1151
Hsp92II CATG 6 cut(s) 139, 145, 260, 623, 798, 1019
HspAI GCGC 2 cut(s) 292, 367
Ksp22I TGATCA 1 cut(s) 1114
Kzo9I GATC 5 cut(s) 305, 586, 592, 925, 1114
LmnI GCTCC 1 cut(s) 798
Lsp1109I GCAGC 2 cut(s) 617, 1132
MabI ACCWGGT 1 cut(s) 889
MaeI CTAG 2 cut(s) 192, 995
MaeIII GTNAC 3 cut(s) 500, 613, 772
MalI GATC 5 cut(s) 307, 588, 594, 927, 1116
MboI GATC 5 cut(s) 305, 586, 592, 925, 1114
MboII GAAGA 5 cut(s) 170, 387, 393, 554, 754
MflI RGATCY 2 cut(s) 586, 592
MhlI GDGCHC 1 cut(s) 1156
MlsI TGGCCA 1 cut(s) 140
MluCI AATT 7 cut(s) 262, 274, 376, 747, 859, 1071, 1133
MluNI TGGCCA 1 cut(s) 140
MlyI GAGTC 2 cut(s) 147, 621
MmeI TCCRAC 2 cut(s) 979, 1026
Mox20I TGGCCA 1 cut(s) 140
MscI TGGCCA 1 cut(s) 140
MseI TTAA 2 cut(s) 174, 930
MslI CAYNNNNRTG 2 cut(s) 140, 620
Msp20I TGGCCA 1 cut(s) 140
MspA1I CMGCKG 2 cut(s) 448, 953
MspI CCGG 4 cut(s) 317, 372, 432, 472
MspR9I CCNGG 2 cut(s) 891, 1107
MvaI CCWGG 2 cut(s) 891, 1107
MvnI CGCG 1 cut(s) 292
MwoI GCNNNNNNNGC 7 cut(s) 137, 326, 432, 435, 736, 1126, 1151
NcoI CCATGG 2 cut(s) 141, 794
NdeI CATATG 1 cut(s) 922
NdeII GATC 5 cut(s) 305, 586, 592, 925, 1114
NlaIII CATG 6 cut(s) 139, 145, 260, 623, 798, 1019
NlaIV GGNNCC 2 cut(s) 430, 594
NmuCI GTSAC 2 cut(s) 500, 613
NspI RCATGY 1 cut(s) 1019
OliI CACNNNNGTG 1 cut(s) 620
PfeI GAWTC 2 cut(s) 169, 577
PkrI GCNGC 4 cut(s) 428, 437, 632, 1147
PleI GAGTC 2 cut(s) 147, 620
PpsI GAGTC 2 cut(s) 147, 620
Psp124BI GAGCTC 1 cut(s) 1156
Psp6I CCWGG 2 cut(s) 889, 1105
PspFI CCCAGC 1 cut(s) 187
PspGI CCWGG 2 cut(s) 889, 1105
PspN4I GGNNCC 2 cut(s) 430, 594
PspPI GGNCC 2 cut(s) 908, 1103
PsuI RGATCY 2 cut(s) 586, 592
PvuII CAGCTG 2 cut(s) 448, 953
RsaI GTAC 1 cut(s) 1060
RsaNI GTAC 1 cut(s) 1059
RseI CAYNNNNRTG 2 cut(s) 140, 620
SacI GAGCTC 1 cut(s) 1156
SaqAI TTAA 2 cut(s) 174, 930
SatI GCNGC 4 cut(s) 427, 436, 631, 1146
Sau3AI GATC 5 cut(s) 305, 586, 592, 925, 1114
Sau96I GGNCC 2 cut(s) 908, 1103
SchI GAGTC 2 cut(s) 147, 621
ScrFI CCNGG 2 cut(s) 891, 1107
SduI GDGCHC 1 cut(s) 1156
SexAI ACCWGGT 1 cut(s) 889
SfcI CTRYAG 2 cut(s) 765, 948
SgrAI CRCCGGYG 1 cut(s) 431
SinI GGWCC 1 cut(s) 908
SmiMI CAYNNNNRTG 2 cut(s) 140, 620
Sse9I AATT 7 cut(s) 262, 274, 376, 747, 859, 1071, 1133
SsiI CCGC 4 cut(s) 426, 435, 661, 1129
SspMI CTAG 2 cut(s) 192, 995
SstI GAGCTC 1 cut(s) 1156
StyD4I CCNGG 2 cut(s) 889, 1105
StyI CCWWGG 2 cut(s) 141, 794
TaqI TCGA 3 cut(s) 167, 468, 1092
TaqII GACCGA 1 cut(s) 1078
TasI AATT 7 cut(s) 262, 274, 376, 747, 859, 1071, 1133
TatI WGTACW 1 cut(s) 1058
TauI GCSGC 2 cut(s) 429, 438
TfiI GAWTC 2 cut(s) 169, 577
Tru1I TTAA 2 cut(s) 174, 930
Tru9I TTAA 2 cut(s) 174, 930
TscAI CASTG 1 cut(s) 732
TseFI GTSAC 2 cut(s) 500, 613
TseI GCWGC 2 cut(s) 630, 1145
Tsp45I GTSAC 2 cut(s) 500, 613
TspDTI ATGAA 5 cut(s) 17, 555, 723, 801, 821
TspRI CASTG 1 cut(s) 732
VpaK11BI GGWCC 1 cut(s) 908
XapI RAATTY 3 cut(s) 262, 376, 1071
XceI RCATGY 1 cut(s) 1019
XspI CTAG 2 cut(s) 192, 995
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.