pycom03g14750

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0009402
Physical Location & Seq
Reverse (-)
16061018 .. 16061728
711 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g14750.1

Sequence Viewer

Length: 384 bp
ATGTCAGCCCGCGATAGCAAGGCCGTCGATCACATTTTATTACACTCTGTTTTGACGACCACCAAAAACAAGGGCGGCGGAGAGGCTGATTCTGATTTTGTCCAAACCACACGATCCATCTATTTGGAAGATCTCATTCTTTCCTCGGATTTGCTTTTATCTCGATTGTTAGGGAAGATGAAGAAAGGGTTGCACCCTCAGATGCAATGGATATCGTACGTGACGCAGAGCGGCAGATTGATGCAGGTTATGATGACCAAAATCCACCCCGTTGGTAAAGTCTACCACTTGAAGGCGAAACGCCAAATGGCTGAGAACATCGGCCAGGTTGCCAAGTTTAAGCAGCGGTACGAGAAGCAGATTGCCCCGAAGGACGAGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

128

Amino Acids

14.41

Weight (kDa)

9.8

Isoelectric Point (pI)

45.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 235
AccBSI CCGCTC 1 cut(s) 231
AccI GTMKAC 1 cut(s) 282
AccII CGCG 1 cut(s) 12
AciI CCGC 5 cut(s) 10, 75, 78, 231, 346
AclWI GGATC 1 cut(s) 108
AcoI YGGCCR 1 cut(s) 322
AfaI GTAC 2 cut(s) 218, 350
AfiI CCNNNNNNNGG 1 cut(s) 292
AgsI TTSAA 1 cut(s) 292
AjnI CCWGG 1 cut(s) 324
AlwI GGATC 1 cut(s) 108
AoxI GGCC 2 cut(s) 21, 322
ApeKI GCWGC 1 cut(s) 343
BbvI GCAGC 1 cut(s) 355
BccI CCATC 1 cut(s) 125
BceAI ACGGC 1 cut(s) 8
BcgI CGANNNNNNTGC 2 cut(s) 7, 41
BciT130I CCWGG 1 cut(s) 326
BfuAI ACCTGC 1 cut(s) 235
BglII AGATCT 1 cut(s) 130
BisI GCNGC 3 cut(s) 76, 232, 344
BlsI GCNGC 3 cut(s) 77, 233, 345
Bme1390I CCNGG 1 cut(s) 326
BmrFI CCNGG 1 cut(s) 326
BmsI GCATC 2 cut(s) 192, 231
BsaAI YACGTR 1 cut(s) 220
BsaJI CCNNGG 1 cut(s) 144
Bsc4I CCNNNNNNNGG 1 cut(s) 292
Bse3DI GCAATG 1 cut(s) 212
BseBI CCWGG 1 cut(s) 326
BseDI CCNNGG 1 cut(s) 144
BseLI CCNNNNNNNGG 1 cut(s) 292
BseMI GCAATG 1 cut(s) 212
BseMII CTCAG 2 cut(s) 212, 303
BseXI GCAGC 1 cut(s) 355
Bsh1236I CGCG 1 cut(s) 12
BshFI GGCC 2 cut(s) 23, 324
BsiWI CGTACG 1 cut(s) 216
BslI CCNNNNNNNGG 1 cut(s) 292
BsnI GGCC 2 cut(s) 23, 324
Bsp143I GATC 3 cut(s) 28, 113, 130
BspACI CCGC 5 cut(s) 10, 75, 78, 231, 346
BspANI GGCC 2 cut(s) 23, 324
BspCNI CTCAG 2 cut(s) 211, 304
BspFNI CGCG 1 cut(s) 12
BspMI ACCTGC 1 cut(s) 235
BspPI GGATC 1 cut(s) 108
BsrBI CCGCTC 1 cut(s) 231
BsrDI GCAATG 1 cut(s) 212
BssECI CCNNGG 1 cut(s) 144
BssMI GATC 3 cut(s) 28, 113, 130
Bst2UI CCWGG 1 cut(s) 326
BstBAI YACGTR 1 cut(s) 220
BstC8I GCNNGC 1 cut(s) 10
BstDEI CTNAG 2 cut(s) 198, 312
BstFNI CGCG 1 cut(s) 12
BstKTI GATC 3 cut(s) 31, 116, 133
BstMBI GATC 3 cut(s) 28, 113, 130
BstNI CCWGG 1 cut(s) 326
BstSCI CCNGG 1 cut(s) 324
BstUI CGCG 1 cut(s) 12
BstV1I GCAGC 1 cut(s) 355
BstX2I RGATCY 1 cut(s) 130
BstXI CCANNNNNNTGG 2 cut(s) 124, 272
BstYI RGATCY 1 cut(s) 130
BsuRI GGCC 2 cut(s) 23, 324
BveI ACCTGC 1 cut(s) 235
Cac8I GCNNGC 1 cut(s) 10
CseI GACGC 1 cut(s) 232
Csp6I GTAC 2 cut(s) 217, 349
CviJI RGCY 6 cut(s) 8, 23, 86, 311, 324, 379
CviKI_1 RGCY 6 cut(s) 8, 23, 86, 311, 324, 379
CviQI GTAC 2 cut(s) 217, 349
DdeI CTNAG 2 cut(s) 198, 312
DpnI GATC 3 cut(s) 30, 115, 132
DpnII GATC 3 cut(s) 28, 113, 130
EaeI YGGCCR 1 cut(s) 322
EciI GGCGGA 1 cut(s) 93
Eco32I GATATC 1 cut(s) 213
EcoRII CCWGG 1 cut(s) 324
EcoRV GATATC 1 cut(s) 213
FaiI YATR 1 cut(s) 251
FauI CCCGC 1 cut(s) 17
FblI GTMKAC 1 cut(s) 282
Fnu4HI GCNGC 3 cut(s) 76, 232, 344
Fsp4HI GCNGC 3 cut(s) 76, 232, 344
GluI GCNGC 3 cut(s) 76, 232, 344
HaeIII GGCC 2 cut(s) 23, 324
HgaI GACGC 1 cut(s) 232
HinfI GANTC 1 cut(s) 89
Hpy166II GTNNAC 1 cut(s) 283
Hpy188I TCNGA 3 cut(s) 94, 148, 201
Hpy188III TCNNGA 1 cut(s) 162
Hpy8I GTNNAC 1 cut(s) 283
Hpy99I CGWCG 1 cut(s) 29
HpyAV CCTTC 2 cut(s) 286, 364
HpyCH4IV ACGT 1 cut(s) 219
HpyCH4V TGCA 3 cut(s) 193, 205, 244
HpyF3I CTNAG 2 cut(s) 198, 312
HpySE526I ACGT 1 cut(s) 219
Kzo9I GATC 3 cut(s) 28, 113, 130
LpnPI CCDG 3 cut(s) 230, 311, 338
Lsp1109I GCAGC 1 cut(s) 355
LweI GCATC 2 cut(s) 192, 231
MaeII ACGT 1 cut(s) 219
MaeIII GTNAC 1 cut(s) 220
MalI GATC 3 cut(s) 30, 115, 132
MbiI CCGCTC 1 cut(s) 231
MboI GATC 3 cut(s) 28, 113, 130
MboII GAAGA 3 cut(s) 140, 187, 193
MflI RGATCY 1 cut(s) 130
MnlI CCTC 3 cut(s) 76, 154, 207
MseI TTAA 1 cut(s) 339
MspA1I CMGCKG 1 cut(s) 346
MspR9I CCNGG 1 cut(s) 326
MvaI CCWGG 1 cut(s) 326
MvnI CGCG 1 cut(s) 12
NdeII GATC 3 cut(s) 28, 113, 130
NmuCI GTSAC 1 cut(s) 220
PfeI GAWTC 1 cut(s) 89
Pfl23II CGTACG 1 cut(s) 216
PkrI GCNGC 3 cut(s) 77, 233, 345
Ppu21I YACGTR 1 cut(s) 220
Psp6I CCWGG 1 cut(s) 324
PspGI CCWGG 1 cut(s) 324
PspLI CGTACG 1 cut(s) 216
PsuI RGATCY 1 cut(s) 130
RsaI GTAC 2 cut(s) 218, 350
RsaNI GTAC 2 cut(s) 217, 349
SaqAI TTAA 1 cut(s) 339
SatI GCNGC 3 cut(s) 76, 232, 344
Sau3AI GATC 3 cut(s) 28, 113, 130
ScrFI CCNGG 1 cut(s) 326
SetI ASST 3 cut(s) 222, 249, 330
SfaNI GCATC 2 cut(s) 192, 231
SsiI CCGC 5 cut(s) 10, 75, 78, 231, 346
StyD4I CCNGG 1 cut(s) 324
TaiI ACGT 1 cut(s) 222
TaqI TCGA 2 cut(s) 27, 163
TauI GCSGC 2 cut(s) 78, 234
TfiI GAWTC 1 cut(s) 89
Tru1I TTAA 1 cut(s) 339
Tru9I TTAA 1 cut(s) 339
TseFI GTSAC 1 cut(s) 220
TseI GCWGC 1 cut(s) 343
Tsp45I GTSAC 1 cut(s) 220
TspDTI ATGAA 1 cut(s) 194
XmiI GTMKAC 1 cut(s) 282
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.