pycom03g15150

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0014255
Physical Location & Seq
Forward (+)
16484010 .. 16487343
3334 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 576 bp
ATGATTTGGAATTGGGGGAGAGAAATGTCGAAGCTGTTTCAGAAGGTTGCTGGGTTCTTTACCAATCGGACTACGGTGGGAATGGACAGAGCTGGCAACCGTTACTTCGCCAGAAAAGAGGAGGTCGATGGCATCATGAAAGAGAAAAGATGGGTGGCATTCAAGGGCGAGGACGATCCGACCTCAATTCCAGTTGAGTGGATATGTTGGCTCAACGGGCAGCGGAAAAGGGCTCCAACACTGGAGGAACTGGCAGAATTGGAAGCAAGACGTGAACGTGTCCGGCAGAATGTTGCTCTTCTAAAGAAAGAAGAAGAGGAAAGGAAAGCAAGAGAATGCAGTACGCACAAAATCATCAACAACGATAAAGTCGGAGGTCCAGATTTGAAAAGTTTCATTCGGCAATTTCCTGGGTCTTCTGAAGGTGGTGTAAATCAGGAAGAATCTGTTGCAACAGAAGGAGTGAGGAACTCCAAAGAGAAAGAGGCAAAGAAAGCAAAAGAAGTTCCAGAGTCTACAGAGCCAACAGGATCCGGTCAAACATTCAGGCCAGGGAAATGGCAGCCACCAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
NDUFA12

Protein Analysis

192

Amino Acids

21.88

Weight (kDa)

9.2

Isoelectric Point (pI)

45.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NDUFA12 PF05071 24 - 99 1.4e-11 NADH ubiquinone oxidoreductase subunit NDUFA12
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014255)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26965 AT4G26965
fragaria_vesca FvH4_3g22870
malus_domestica MD11G1216700.v1.1
prunus_persica Prupe.4G214700_v2.0.a1 Prupe.4G214700_v2.0.a1
pyrus_communis pycom03g15150 pycom11g19080
rosa_chinensis RchiOBHm_Chr5g0040171
rosa_laevigata RLG00000033962
rosa_multiflora Rmu_sc0002908.1_g000018 Rmu_sc0010162.1_g000010
rosa_roxburghii Rroxscaffold_1G00041110
rosa_rugosa Rorug05G0183900
rosa_samantha Rh5CG306900 Rh5DG282200
rosa_wichuraiana Rw5G025280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 515
AciI CCGC 1 cut(s) 223
AclWI GGATC 3 cut(s) 170, 525, 538
AcuI CTGAAG 1 cut(s) 441
AfaI GTAC 1 cut(s) 343
AflIII ACRYGT 1 cut(s) 277
AgsI TTSAA 2 cut(s) 163, 388
AjiI CACGTC 1 cut(s) 272
AjnI CCWGG 2 cut(s) 409, 550
AluBI AGCT 2 cut(s) 34, 92
AluI AGCT 2 cut(s) 34, 92
AlwI GGATC 3 cut(s) 170, 525, 538
AoxI GGCC 1 cut(s) 548
ApeKI GCWGC 2 cut(s) 220, 562
Asp700I GAANNNNTTC 1 cut(s) 392
AspS9I GGNCC 1 cut(s) 377
AvaII GGWCC 1 cut(s) 377
BamHI GGATCC 1 cut(s) 530
BanII GRGCYC 1 cut(s) 235
BbsI GAAGAC 1 cut(s) 408
BbvI GCAGC 1 cut(s) 232
BccI CCATC 2 cut(s) 122, 144
BciT130I CCWGG 2 cut(s) 411, 552
BfmI CTRYAG 1 cut(s) 516
BisI GCNGC 2 cut(s) 221, 563
BlsI GCNGC 2 cut(s) 222, 564
Bme1390I CCNGG 2 cut(s) 411, 552
Bme18I GGWCC 1 cut(s) 377
BmgBI CACGTC 1 cut(s) 272
BmgT120I GGNCC 1 cut(s) 377
BmiI GGNNCC 2 cut(s) 234, 532
BmrFI CCNGG 2 cut(s) 411, 552
BmsI GCATC 1 cut(s) 141
BpiI GAAGAC 1 cut(s) 408
BpmI CTGGAG 1 cut(s) 263
BsaJI CCNNGG 2 cut(s) 410, 551
BsaWI WCCGGW 1 cut(s) 533
Bse1I ACTGG 3 cut(s) 191, 246, 255
BseBI CCWGG 2 cut(s) 411, 552
BseDI CCNNGG 2 cut(s) 410, 551
BseNI ACTGG 3 cut(s) 191, 246, 255
BseRI GAGGAG 1 cut(s) 134
BseXI GCAGC 1 cut(s) 232
BseYI CCCAGC 1 cut(s) 50
BshFI GGCC 1 cut(s) 550
BsiSI CCGG 2 cut(s) 283, 534
BsmI GAATGC 2 cut(s) 158, 341
BsnI GGCC 1 cut(s) 550
Bsp1286I GDGCHC 1 cut(s) 235
Bsp143I GATC 2 cut(s) 175, 530
BspACI CCGC 1 cut(s) 223
BspANI GGCC 1 cut(s) 550
BspHI TCATGA 1 cut(s) 135
BspLI GGNNCC 2 cut(s) 234, 532
BspPI GGATC 3 cut(s) 170, 525, 538
BspQI GCTCTTC 1 cut(s) 303
BsrI ACTGG 3 cut(s) 191, 246, 255
BssECI CCNNGG 2 cut(s) 410, 551
BssMI GATC 2 cut(s) 175, 530
Bst2UI CCWGG 2 cut(s) 411, 552
Bst4CI ACNGT 2 cut(s) 76, 101
Bst6I CTCTTC 2 cut(s) 303, 309
BstC8I GCNNGC 1 cut(s) 94
BstKTI GATC 2 cut(s) 178, 533
BstMBI GATC 2 cut(s) 175, 530
BstMWI GCNNNNNNNGC 2 cut(s) 217, 494
BstNI CCWGG 2 cut(s) 411, 552
BstSCI CCNGG 2 cut(s) 409, 550
BstSFI CTRYAG 1 cut(s) 516
BstV1I GCAGC 1 cut(s) 232
BstV2I GAAGAC 1 cut(s) 408
BstX2I RGATCY 1 cut(s) 530
BstXI CCANNNNNNTGG 2 cut(s) 198, 558
BstYI RGATCY 1 cut(s) 530
BsuRI GGCC 1 cut(s) 550
BtrI CACGTC 1 cut(s) 272
BtsIMutI CAGTG 1 cut(s) 239
Cac8I GCNNGC 1 cut(s) 94
CciI TCATGA 1 cut(s) 135
Cfr13I GGNCC 1 cut(s) 377
Csp6I GTAC 1 cut(s) 342
CviAII CATG 2 cut(s) 136, 573
CviJI RGCY 7 cut(s) 34, 92, 211, 233, 523, 550, 565
CviKI_1 RGCY 7 cut(s) 34, 92, 211, 233, 523, 550, 565
CviQI GTAC 1 cut(s) 342
DpnI GATC 2 cut(s) 177, 532
DpnII GATC 2 cut(s) 175, 530
Eam1104I CTCTTC 2 cut(s) 303, 309
EarI CTCTTC 2 cut(s) 303, 309
Eco24I GRGCYC 1 cut(s) 235
Eco47I GGWCC 1 cut(s) 377
Eco57I CTGAAG 1 cut(s) 441
EcoRII CCWGG 2 cut(s) 409, 550
EcoT38I GRGCYC 1 cut(s) 235
FaeI CATG 2 cut(s) 139, 576
FaiI YATR 3 cut(s) 137, 205, 574
FatI CATG 2 cut(s) 135, 572
FblI GTMKAC 1 cut(s) 515
Fnu4HI GCNGC 2 cut(s) 221, 563
FriOI GRGCYC 1 cut(s) 235
Fsp4HI GCNGC 2 cut(s) 221, 563
GluI GCNGC 2 cut(s) 221, 563
GsaI CCCAGC 1 cut(s) 54
GsuI CTGGAG 1 cut(s) 263
HaeIII GGCC 1 cut(s) 550
HapII CCGG 2 cut(s) 283, 534
Hin1II CATG 2 cut(s) 139, 576
HinfI GANTC 2 cut(s) 443, 512
HpaII CCGG 2 cut(s) 283, 534
Hpy166II GTNNAC 2 cut(s) 275, 516
Hpy188I TCNGA 5 cut(s) 42, 69, 180, 374, 421
Hpy188III TCNNGA 4 cut(s) 136, 380, 437, 509
Hpy8I GTNNAC 2 cut(s) 275, 516
HpyAV CCTTC 3 cut(s) 37, 416, 452
HpyCH4III ACNGT 2 cut(s) 76, 101
HpyCH4IV ACGT 2 cut(s) 271, 277
HpyCH4V TGCA 2 cut(s) 339, 452
HpyF10VI GCNNNNNNNGC 2 cut(s) 217, 494
HpySE526I ACGT 2 cut(s) 271, 277
Hsp92II CATG 2 cut(s) 139, 576
Kzo9I GATC 2 cut(s) 175, 530
LguI GCTCTTC 1 cut(s) 303
LmnI GCTCC 1 cut(s) 238
Lsp1109I GCAGC 1 cut(s) 232
LweI GCATC 1 cut(s) 141
MaeII ACGT 2 cut(s) 271, 277
MaeIII GTNAC 1 cut(s) 101
MalI GATC 2 cut(s) 177, 532
MboI GATC 2 cut(s) 175, 530
MboII GAAGA 5 cut(s) 290, 323, 326, 408, 452
MflI RGATCY 1 cut(s) 530
MhlI GDGCHC 1 cut(s) 235
MluCI AATT 4 cut(s) 10, 186, 257, 404
MlyI GAGTC 1 cut(s) 521
MmeI TCCRAC 3 cut(s) 203, 260, 352
MnlI CCTC 9 cut(s) 112, 115, 163, 193, 238, 310, 368, 459, 478
MroXI GAANNNNTTC 1 cut(s) 392
MslI CAYNNNNRTG 1 cut(s) 571
MspA1I CMGCKG 1 cut(s) 223
MspI CCGG 2 cut(s) 283, 534
MspR9I CCNGG 2 cut(s) 411, 552
Mva1269I GAATGC 2 cut(s) 158, 341
MvaI CCWGG 2 cut(s) 411, 552
MwoI GCNNNNNNNGC 2 cut(s) 217, 494
NdeII GATC 2 cut(s) 175, 530
NlaIII CATG 2 cut(s) 139, 576
NlaIV GGNNCC 2 cut(s) 234, 532
PagI TCATGA 1 cut(s) 135
PciSI GCTCTTC 1 cut(s) 303
PctI GAATGC 2 cut(s) 158, 341
PdmI GAANNNNTTC 1 cut(s) 392
PfeI GAWTC 1 cut(s) 443
PkrI GCNGC 2 cut(s) 222, 564
PleI GAGTC 1 cut(s) 520
PpsI GAGTC 1 cut(s) 520
Psp6I CCWGG 2 cut(s) 409, 550
PspFI CCCAGC 1 cut(s) 50
PspGI CCWGG 2 cut(s) 409, 550
PspN4I GGNNCC 2 cut(s) 234, 532
PspPI GGNCC 1 cut(s) 377
PsuI RGATCY 1 cut(s) 530
RsaI GTAC 1 cut(s) 343
RsaNI GTAC 1 cut(s) 342
RseI CAYNNNNRTG 1 cut(s) 571
SapI GCTCTTC 1 cut(s) 303
SatI GCNGC 2 cut(s) 221, 563
Sau3AI GATC 2 cut(s) 175, 530
Sau96I GGNCC 1 cut(s) 377
SchI GAGTC 1 cut(s) 521
ScrFI CCNGG 2 cut(s) 411, 552
SduI GDGCHC 1 cut(s) 235
SetI ASST 9 cut(s) 36, 48, 94, 126, 185, 274, 280, 379, 427
SfaNI GCATC 1 cut(s) 141
SfcI CTRYAG 1 cut(s) 516
SinI GGWCC 1 cut(s) 377
SmiMI CAYNNNNRTG 1 cut(s) 571
Sse9I AATT 4 cut(s) 10, 186, 257, 404
SsiI CCGC 1 cut(s) 223
StyD4I CCNGG 2 cut(s) 409, 550
TaaI ACNGT 2 cut(s) 76, 101
TaiI ACGT 2 cut(s) 274, 280
TaqI TCGA 2 cut(s) 29, 126
TasI AATT 4 cut(s) 10, 186, 257, 404
TfiI GAWTC 1 cut(s) 443
TscAI CASTG 1 cut(s) 246
TseI GCWGC 2 cut(s) 220, 562
TspDTI ATGAA 2 cut(s) 152, 385
TspRI CASTG 1 cut(s) 246
VpaK11BI GGWCC 1 cut(s) 377
XmiI GTMKAC 1 cut(s) 515
XmnI GAANNNNTTC 1 cut(s) 392
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.