pycom03g18610

Topless-related protein 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0017337
Physical Location & Seq
Forward (+)
19558038 .. 19560246
2209 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g18610.12

Sequence Viewer

Length: 1674 bp
ATGACTCCCCCTCTAACTTGGTCTCGATTCCTTGAGACTTGGAAATCCCTCACCCCAAACATAGCCAAAAGAAGTCAAAATTTTGATTCAGGGGCCAAACTGAGAATCGGAACATCACCCCCAAAATATGCAAAAACAATCTACGAACGAATAGCAACACATAATCTTCAAAGAGTACTGCAAATTAACATGTTCCATTTAACAAGGATCCCCATGCAGTCTGCTTTCTGTCAATATGTGCACTACCTGAAAGAACTCATGCTGCGCACCAAAGTAAAGACACGACTAATCGCAACCACGATCCGACCACAGCAATATGCCAATATTGCTCCTGATCACTCAATCACGACAATGGCCTATGATGCAGATGGTAAAAGGTTGGTATACCACTCCTTTCAAATGTTATTGCGACCTACACCTATATTCCAGGCAACAAGAAAATTCAATCTTTACATTAAACAATCTCATGTTGTTGAATGGAATGAAAATGATGGTGTAATAAAGAGGAATTACGTAGGATTTCAAAAACCATCTTTGGGTGTTGTTCAGTTTGATACAAGCAAGAATAAGTATTTGGCTGTTGGCGATGACTATGCACTCAAAGTTTGGGGCATGGATAAAATCAATATTTTGACCACTGTTGATGCTGAAGGCGGTCTACCAAATGCACAAGGGATTGCTTTATTTTTTAGGAAACTTGGATTCACAAAGGATACCACAATCTATTTGACCGAATCAAAATGGGACAGCAGTCTTGATGATCTGAAAGACATCTTCCCGAAGACGTATATCAAGGTGCACACATCTGTTATGCCTTGCATTTTTTACCATGTTTCAGTTTCTTTCCGAAACCCATATCATTGCAATGCTATTTCGGATGTAGATTTTGTACTTAATGAGTCTGGAATTCATTGCAGGATGAAAGCTGCTTGGAACCCCTACTGGTGCATTTCTAGATCTGTTCTCTACTGTTCGAGAAAGCTGCTTCACTGCTTAAGAGAAGAGCAAGAGATAATGTGCACGAAAATTCCTGAAATCAGATACATATCAAACCAGGATGGACTCCTGCAGTTTGATAATTGTAGTATTTCATACTCTTTTTCTAAGGGGGTTGCAGCACAAAACTTTACGATTATGCAAGAGATACCGATATTGCTAATCACATGTTGTTTTCGGGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTGATTTTTATGTGGTGTTTATATGTGCAGGTGACAGTCGCCTTGAGGTTGGTCTCCAACGGTTTCTCATCAACTCATCTACCGAATCCACAAAACCAACTGCACAAATCCTAAATTTTTCTTGGATCGATCTGGGTGTACGTGCGTGTGTGGAAATAATAAATGTTAAATTTGAATATATTGATGTGTTGTTAAGAATACAAGATAAAGCCTCTCTTATAGAGAAGCCGAGAAGGAGGCAAAGACGCAAGAAGCAATTACAGAAAGTGGAAAGGAAAAATAAGGAAAAAAAAAGGTGGGGTATGGTGGAGATGATGGTAAATGTATTTGGCTTCTATATATGTTCTCTTAAGACCAACTCCAAACCCAAATTACCCTCTAAAAGCCAAATTACCCTCCAAAATTTCCCCCCAAACCCACTCCAACTCAAGGGAAAATTTTGGGCTAAATCCCAGAAATCGGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04934 map04934
Pfam Domains
Protein Families
WD40

Protein Analysis

558

Amino Acids

64.87

Weight (kDa)

9.59

Isoelectric Point (pI)

45.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1226
Acc16I TGCGCA 1 cut(s) 266
Acc36I ACCTGC 1 cut(s) 1226
AccI GTMKAC 2 cut(s) 384, 658
AciI CCGC 1 cut(s) 654
AclWI GGATC 4 cut(s) 202, 215, 295, 1340
AcsI RAATTY 8 cut(s) 79, 440, 906, 1026, 1321, 1376, 1609, 1643
AcuI CTGAAG 1 cut(s) 669
AfaI GTAC 3 cut(s) 177, 891, 1347
AfiI CCNNNNNNNGG 3 cut(s) 536, 1636, 1666
AflII CTTAAG 2 cut(s) 994, 1556
AflIII ACRYGT 2 cut(s) 189, 1163
AgsI TTSAA 6 cut(s) 170, 398, 445, 476, 524, 1382
AjnI CCWGG 2 cut(s) 426, 1053
AjuI GAANNNNNNNTTGG 2 cut(s) 557, 589
AluBI AGCT 2 cut(s) 926, 982
AluI AGCT 2 cut(s) 926, 982
Alw21I GWGCWC 3 cut(s) 243, 801, 1022
Alw26I GTCTC 3 cut(s) 27, 29, 1265
Alw44I GTGCAC 3 cut(s) 239, 797, 1018
AlwI GGATC 4 cut(s) 202, 215, 295, 1340
AoxI GGCC 2 cut(s) 93, 354
ApaLI GTGCAC 3 cut(s) 239, 797, 1018
ApeKI GCWGC 4 cut(s) 262, 926, 982, 1115
ApoI RAATTY 8 cut(s) 79, 440, 906, 1026, 1321, 1376, 1609, 1643
AspLEI GCGC 1 cut(s) 267
AspS9I GGNCC 1 cut(s) 93
AsuHPI GGTGA 3 cut(s) 43, 108, 1250
BaeGI GKGCMC 3 cut(s) 243, 801, 1022
BamHI GGATCC 1 cut(s) 207
BarI GAAGNNNNNNTAC 2 cut(s) 642, 674
BbsI GAAGAC 1 cut(s) 788
Bbv12I GWGCWC 3 cut(s) 243, 801, 1022
BbvI GCAGC 4 cut(s) 249, 913, 969, 1127
BccI CCATC 5 cut(s) 362, 485, 538, 1052, 1516
BcgI CGANNNNNNTGC 4 cut(s) 575, 609, 964, 998
BciT130I CCWGG 2 cut(s) 428, 1055
BciVI GTATCC 1 cut(s) 706
BclI TGATCA 1 cut(s) 334
BcoDI GTCTC 3 cut(s) 27, 29, 1265
BfaI CTAG 1 cut(s) 954
BfmI CTRYAG 1 cut(s) 1067
BfrI CTTAAG 2 cut(s) 994, 1556
BfuAI ACCTGC 1 cut(s) 1226
BfuI GTATCC 1 cut(s) 706
BglII AGATCT 1 cut(s) 956
BisI GCNGC 4 cut(s) 263, 927, 983, 1116
BlsI GCNGC 4 cut(s) 264, 928, 984, 1117
BmcAI AGTACT 1 cut(s) 177
Bme1390I CCNGG 2 cut(s) 428, 1055
BmgT120I GGNCC 1 cut(s) 93
BmiI GGNNCC 3 cut(s) 94, 209, 935
BmrFI CCNGG 2 cut(s) 428, 1055
BmsI GCATC 2 cut(s) 352, 634
BoxI GACNNNNGTC 1 cut(s) 750
BpiI GAAGAC 1 cut(s) 788
BpuEI CTTGAG 3 cut(s) 53, 1271, 1619
Bsa29I ATCGAT 1 cut(s) 1335
BsaAI YACGTR 2 cut(s) 514, 1349
BsaBI GATNNNNATC 1 cut(s) 1045
BsaI GGTCTC 2 cut(s) 27, 1265
Bsc4I CCNNNNNNNGG 3 cut(s) 536, 1636, 1666
Bse1I ACTGG 1 cut(s) 947
Bse3DI GCAATG 3 cut(s) 859, 871, 910
Bse8I GATNNNNATC 1 cut(s) 1045
BseBI CCWGG 2 cut(s) 428, 1055
BseCI ATCGAT 1 cut(s) 1335
BseGI GGATG 3 cut(s) 883, 924, 1063
BseJI GATNNNNATC 1 cut(s) 1045
BseLI CCNNNNNNNGG 3 cut(s) 536, 1636, 1666
BseMI GCAATG 3 cut(s) 859, 871, 910
BseMII CTCAG 1 cut(s) 92
BseNI ACTGG 1 cut(s) 947
BseSI GKGCMC 3 cut(s) 243, 801, 1022
BseXI GCAGC 4 cut(s) 249, 913, 969, 1127
BsgI GTGCAG 2 cut(s) 1254, 1293
BshFI GGCC 2 cut(s) 95, 356
BshVI ATCGAT 1 cut(s) 1335
BsiHKAI GWGCWC 3 cut(s) 243, 801, 1022
BslFI GGGAC 1 cut(s) 758
BslI CCNNNNNNNGG 3 cut(s) 536, 1636, 1666
BsmAI GTCTC 3 cut(s) 27, 29, 1265
BsmFI GGGAC 1 cut(s) 758
BsnI GGCC 2 cut(s) 95, 356
Bso31I GGTCTC 2 cut(s) 27, 1265
Bsp1286I GDGCHC 3 cut(s) 243, 801, 1022
Bsp143I GATC 7 cut(s) 207, 300, 334, 760, 956, 1332, 1336
BspACI CCGC 1 cut(s) 654
BspANI GGCC 2 cut(s) 95, 356
BspCNI CTCAG 1 cut(s) 93
BspDI ATCGAT 1 cut(s) 1335
BspLI GGNNCC 3 cut(s) 94, 209, 935
BspMAI CTGCAG 1 cut(s) 1071
BspMI ACCTGC 1 cut(s) 1226
BspPI GGATC 4 cut(s) 202, 215, 295, 1340
BspQI GCTCTTC 1 cut(s) 996
BspTI CTTAAG 2 cut(s) 994, 1556
BspTNI GGTCTC 2 cut(s) 27, 1265
BsrDI GCAATG 3 cut(s) 859, 871, 910
BsrI ACTGG 1 cut(s) 947
BssMI GATC 7 cut(s) 207, 300, 334, 760, 956, 1332, 1336
BssNAI GTATAC 1 cut(s) 385
Bst1107I GTATAC 1 cut(s) 385
Bst2UI CCWGG 2 cut(s) 428, 1055
Bst4CI ACNGT 4 cut(s) 640, 971, 1244, 1269
Bst6I CTCTTC 1 cut(s) 996
BstAFI CTTAAG 2 cut(s) 994, 1556
BstBAI YACGTR 2 cut(s) 514, 1349
BstDEI CTNAG 2 cut(s) 101, 1104
BstF5I GGATG 3 cut(s) 883, 924, 1063
BstHHI GCGC 1 cut(s) 267
BstKTI GATC 7 cut(s) 210, 303, 337, 763, 959, 1335, 1339
BstMAI GTCTC 3 cut(s) 27, 29, 1265
BstMBI GATC 7 cut(s) 207, 300, 334, 760, 956, 1332, 1336
BstMWI GCNNNNNNNGC 2 cut(s) 326, 362
BstNI CCWGG 2 cut(s) 428, 1055
BstNSI RCATGY 2 cut(s) 193, 1167
BstPAI GACNNNNGTC 1 cut(s) 750
BstSCI CCNGG 2 cut(s) 426, 1053
BstSFI CTRYAG 1 cut(s) 1067
BstSLI GKGCMC 3 cut(s) 243, 801, 1022
BstSNI TACGTA 1 cut(s) 514
BstV1I GCAGC 4 cut(s) 249, 913, 969, 1127
BstV2I GAAGAC 1 cut(s) 788
BstX2I RGATCY 2 cut(s) 207, 956
BstYI RGATCY 2 cut(s) 207, 956
BstZ17I GTATAC 1 cut(s) 385
Bsu15I ATCGAT 1 cut(s) 1335
BsuI GTATCC 1 cut(s) 706
BsuRI GGCC 2 cut(s) 95, 356
BsuTUI ATCGAT 1 cut(s) 1335
BtgZI GCGATG 1 cut(s) 600
BtsCI GGATG 3 cut(s) 883, 924, 1063
BtsI GCAGTG 1 cut(s) 988
BtsIMutI CAGTG 2 cut(s) 636, 988
BveI ACCTGC 1 cut(s) 1226
CfoI GCGC 1 cut(s) 267
Cfr13I GGNCC 1 cut(s) 93
ClaI ATCGAT 1 cut(s) 1335
CseI GACGC 1 cut(s) 1461
Csp6I GTAC 3 cut(s) 176, 890, 1346
CviAII CATG 7 cut(s) 190, 214, 259, 467, 613, 830, 1164
CviQI GTAC 3 cut(s) 176, 890, 1346
DdeI CTNAG 2 cut(s) 101, 1104
DpnI GATC 7 cut(s) 209, 302, 336, 762, 958, 1334, 1338
DpnII GATC 7 cut(s) 207, 300, 334, 760, 956, 1332, 1336
Eam1104I CTCTTC 1 cut(s) 996
EarI CTCTTC 1 cut(s) 996
Eco105I TACGTA 1 cut(s) 514
Eco31I GGTCTC 2 cut(s) 27, 1265
Eco57I CTGAAG 1 cut(s) 669
EcoRI GAATTC 1 cut(s) 906
EcoRII CCWGG 2 cut(s) 426, 1053
FaeI CATG 7 cut(s) 193, 217, 262, 470, 616, 833, 1167
FalI AAGNNNNNCTT 2 cut(s) 1407, 1439
FaqI GGGAC 1 cut(s) 758
FatI CATG 7 cut(s) 189, 213, 258, 466, 612, 829, 1163
FbaI TGATCA 1 cut(s) 334
FblI GTMKAC 2 cut(s) 384, 658
Fnu4HI GCNGC 4 cut(s) 263, 927, 983, 1116
FokI GGATG 3 cut(s) 890, 931, 1070
Fsp4HI GCNGC 4 cut(s) 263, 927, 983, 1116
FspBI CTAG 1 cut(s) 954
FspI TGCGCA 1 cut(s) 266
GlaI GCGC 1 cut(s) 266
GluI GCNGC 4 cut(s) 263, 927, 983, 1116
HaeIII GGCC 2 cut(s) 95, 356
HgaI GACGC 1 cut(s) 1461
HhaI GCGC 1 cut(s) 267
Hin1II CATG 7 cut(s) 193, 217, 262, 470, 616, 833, 1167
Hin6I GCGC 1 cut(s) 265
HinP1I GCGC 1 cut(s) 265
HinfI GANTC 9 cut(s) 4, 27, 86, 105, 702, 734, 899, 1062, 1292
HphI GGTGA 3 cut(s) 43, 108, 1250
Hpy166II GTNNAC 6 cut(s) 241, 385, 659, 799, 1020, 1346
Hpy188I TCNGA 7 cut(s) 110, 305, 765, 848, 877, 1040, 1669
Hpy188III TCNNGA 9 cut(s) 24, 332, 346, 755, 778, 903, 954, 975, 1031
Hpy8I GTNNAC 6 cut(s) 241, 385, 659, 799, 1020, 1346
HpyAV CCTTC 2 cut(s) 644, 1434
HpyCH4III ACNGT 4 cut(s) 640, 971, 1244, 1269
HpyCH4IV ACGT 3 cut(s) 513, 785, 1348
HpyF10VI GCNNNNNNNGC 2 cut(s) 326, 362
HpyF3I CTNAG 2 cut(s) 101, 1104
HpySE526I ACGT 3 cut(s) 513, 785, 1348
Hsp92II CATG 7 cut(s) 193, 217, 262, 470, 616, 833, 1167
HspAI GCGC 1 cut(s) 265
Ksp22I TGATCA 1 cut(s) 334
Kzo9I GATC 7 cut(s) 207, 300, 334, 760, 956, 1332, 1336
LguI GCTCTTC 1 cut(s) 996
LmnI GCTCC 1 cut(s) 334
Lsp1109I GCAGC 4 cut(s) 249, 913, 969, 1127
LweI GCATC 2 cut(s) 352, 634
MaeI CTAG 1 cut(s) 954
MaeII ACGT 3 cut(s) 513, 785, 1348
MaeIII GTNAC 1 cut(s) 1238
MalI GATC 7 cut(s) 209, 302, 336, 762, 958, 1334, 1338
MboI GATC 7 cut(s) 207, 300, 334, 760, 956, 1332, 1336
MboII GAAGA 4 cut(s) 158, 766, 793, 1013
MflI RGATCY 2 cut(s) 207, 956
MhlI GDGCHC 3 cut(s) 243, 801, 1022
MlyI GAGTC 2 cut(s) 908, 1056
MmeI TCCRAC 3 cut(s) 328, 1288, 1654
MnlI CCTC 8 cut(s) 21, 59, 498, 1246, 1429, 1437, 1594, 1613
MseI TTAA 8 cut(s) 186, 200, 456, 894, 995, 1374, 1400, 1557
MslI CAYNNNNRTG 2 cut(s) 350, 864
MspCI CTTAAG 2 cut(s) 994, 1556
MspR9I CCNGG 2 cut(s) 428, 1055
MvaI CCWGG 2 cut(s) 428, 1055
MwoI GCNNNNNNNGC 2 cut(s) 326, 362
NdeII GATC 7 cut(s) 207, 300, 334, 760, 956, 1332, 1336
NlaIII CATG 7 cut(s) 193, 217, 262, 470, 616, 833, 1167
NlaIV GGNNCC 3 cut(s) 94, 209, 935
NmeAIII GCCGAG 1 cut(s) 1461
NmuCI GTSAC 1 cut(s) 1238
NsbI TGCGCA 1 cut(s) 266
NspI RCATGY 2 cut(s) 193, 1167
PaqCI CACCTGC 1 cut(s) 1226
PciI ACATGT 2 cut(s) 189, 1163
PciSI GCTCTTC 1 cut(s) 996
PfeI GAWTC 6 cut(s) 27, 86, 105, 702, 734, 1292
PkrI GCNGC 4 cut(s) 264, 928, 984, 1117
PleI GAGTC 2 cut(s) 907, 1056
PpsI GAGTC 2 cut(s) 907, 1056
Ppu21I YACGTR 2 cut(s) 514, 1349
PscI ACATGT 2 cut(s) 189, 1163
PshAI GACNNNNGTC 1 cut(s) 750
Psp6I CCWGG 2 cut(s) 426, 1053
PspGI CCWGG 2 cut(s) 426, 1053
PspN4I GGNNCC 3 cut(s) 94, 209, 935
PspPI GGNCC 1 cut(s) 93
PstI CTGCAG 1 cut(s) 1071
PsuI RGATCY 2 cut(s) 207, 956
RsaI GTAC 3 cut(s) 177, 891, 1347
RsaNI GTAC 3 cut(s) 176, 890, 1346
RseI CAYNNNNRTG 2 cut(s) 350, 864
SapI GCTCTTC 1 cut(s) 996
SaqAI TTAA 8 cut(s) 186, 200, 456, 894, 995, 1374, 1400, 1557
SatI GCNGC 4 cut(s) 263, 927, 983, 1116
Sau3AI GATC 7 cut(s) 207, 300, 334, 760, 956, 1332, 1336
Sau96I GGNCC 1 cut(s) 93
ScaI AGTACT 1 cut(s) 177
SchI GAGTC 2 cut(s) 908, 1056
ScrFI CCNGG 2 cut(s) 428, 1055
SduI GDGCHC 3 cut(s) 243, 801, 1022
SfaNI GCATC 2 cut(s) 352, 634
SfcI CTRYAG 1 cut(s) 1067
SmiMI CAYNNNNRTG 2 cut(s) 350, 864
SmlI CTYRAG 5 cut(s) 32, 994, 1250, 1556, 1634
SmoI CTYRAG 5 cut(s) 32, 994, 1250, 1556, 1634
SnaBI TACGTA 1 cut(s) 514
SsiI CCGC 1 cut(s) 654
SspI AATATT 2 cut(s) 325, 628
SspMI CTAG 1 cut(s) 954
StyD4I CCNGG 2 cut(s) 426, 1053
TaaI ACNGT 4 cut(s) 640, 971, 1244, 1269
TaiI ACGT 3 cut(s) 516, 788, 1351
TaqI TCGA 3 cut(s) 25, 974, 1335
TaqII GACCGA 1 cut(s) 746
TatI WGTACW 2 cut(s) 175, 889
TfiI GAWTC 6 cut(s) 27, 86, 105, 702, 734, 1292
Tru1I TTAA 8 cut(s) 186, 200, 456, 894, 995, 1374, 1400, 1557
Tru9I TTAA 8 cut(s) 186, 200, 456, 894, 995, 1374, 1400, 1557
TscAI CASTG 2 cut(s) 643, 995
TseFI GTSAC 1 cut(s) 1238
TseI GCWGC 4 cut(s) 262, 926, 982, 1115
Tsp45I GTSAC 1 cut(s) 1238
TspDTI ATGAA 4 cut(s) 498, 899, 935, 1080
TspRI CASTG 2 cut(s) 643, 995
Vha464I CTTAAG 2 cut(s) 994, 1556
VneI GTGCAC 3 cut(s) 239, 797, 1018
XapI RAATTY 8 cut(s) 79, 440, 906, 1026, 1321, 1376, 1609, 1643
XbaI TCTAGA 1 cut(s) 953
XceI RCATGY 2 cut(s) 193, 1167
XmiI GTMKAC 2 cut(s) 384, 658
XspI CTAG 1 cut(s) 954
ZrmI AGTACT 1 cut(s) 177
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.