pycom04g07400

Asparagine-specific endopeptidase involved in the processing of vacuolar seed protein precursors into the mature forms

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0015210
Physical Location & Seq
Forward (+)
7869306 .. 7875033
5728 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g07400.1

Sequence Viewer

Length: 1185 bp
ATGGCGGTCCAACATCAGTCCAGGTTGATGATTGGTAACTATACTGTGTCGTGGATATCGTTGTTTTTGTTTGTACTGGTCATCCAAGCTATAGGTGGAGGCCGAGCCGCCCGGTTGGAGCGGTGGGACTCTGGAATCCGGTTGCCATCCGAGAAGGATAAACCGGAAGATGTTCATCACAAAGAACCGGGTACAAGATGGGCGGTTCTTGTAGCCGGTTCAAACGGGTATGGAAACTACAGGCATCAGGCAGATATTTGCCACGCATATCAGTTACTGAAAAAAGGTGGGCTGAAGGAGGAGAACATAGTGGTATTTATGTACGACGACATAGCCAAGCACTCAATGAATCCGAGGCCGGGAGTTATCATCAACCATCCTCAGGGTGGTGATGTTTATGCCGGCGTGCCTAAGGATTACACTGGTGAGCAAGTAACTGCTGCCAATCTGTATGCGGTCCTCCTTGGGGACAAAAAGGCCGTGAAAGGCGGAAGTGGGAAGGTTGTGGATAGCAAACCTAATGATAGGATCTTTTTATACTACTCAGATCATGGGGGTCCTGGTGTCCTTGGGATGCCAAACATGCCTTTCCTTTATGCCATGGATTTTATTGAGGTTTTGAAGAAGAAACATGCATCGGGGAGCTATAAAGAGATGGTTATTTATGTAGAAGCTTGTGAGAGTGGGAGCATATTCGAAGGCATAATGCCGAAAGACCTGAACATTTATGTGACTACAGCATCAAATGCACAAGAGAACAGCTTTGGAACTTATTGTCCTGGGATGGACCCCCCACCACCACCTGAATATATCACTTGCTTGGGCGACTTGTACAGTATTTCTTGGATGGAAGATAGCGAGAGGCACAATCTGAAAAAAGAAACAATTGAACAACAGTATCAGGCGGTAAAGCAACGGACTTCCAATTCAAACAATTACGATGTGGGATCGCATGTGATGGAATATGGGAGCAAAAACATTACACAAGAAAAGCTGTATTTGTACCTAGGTTTTGATCCTGCCTCTGTGAACTTGCCTCCTAACAACGGCCAATTAGAAAAGCCTATGGAAGTTGTCAACCAGAGAGATGCAGAGATTTTCTACATGTGGCAAATGTACAAAAGATCAGAACGTGGATCTGGAAAAAGTAGAGAAATCCTCAATGATCGATCAAAGAAACAATGA

Protein Analysis

395

Amino Acids

44.27

Weight (kDa)

6.61

Isoelectric Point (pI)

48.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C13 PF01650 66 - 337 3.3e-117 Peptidase C13 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015210)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G62710
fragaria_vesca FvH4_5g00370
malus_domestica MD04G1082300.v1.1
prunus_persica Prupe.5G076300_v2.0.a1
pyrus_communis pycom04g07400
rosa_chinensis RchiOBHm_Chr7g0202151
rosa_laevigata RLG00000003621
rosa_multiflora Rmu_sc0000018.1_g000001 Rmu_sc0003823.1_g000019
rosa_roxburghii Rroxscaffold_3G00254800
rosa_rugosa Rorug07G0068500
rosa_samantha Rh7AG195900 Rh7BG197200 Rh7CG207000 Rh7DG202500
rosa_wichuraiana Rw7G017160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 121
AciI CCGC 7 cut(s) 5, 108, 121, 203, 455, 489, 905
AclWI GGATC 4 cut(s) 536, 955, 1010, 1144
AcoI YGGCCR 1 cut(s) 1048
AcuI CTGAAG 1 cut(s) 314
AfaI GTAC 6 cut(s) 75, 193, 323, 833, 1004, 1118
AfiI CCNNNNNNNGG 5 cut(s) 359, 382, 386, 466, 1046
AflIII ACRYGT 1 cut(s) 1104
AgsI TTSAA 4 cut(s) 222, 622, 890, 930
AjnI CCWGG 3 cut(s) 20, 559, 778
AloI GAACNNNNNNTCC 2 cut(s) 760, 792
AluBI AGCT 5 cut(s) 89, 645, 674, 762, 994
AluI AGCT 5 cut(s) 89, 645, 674, 762, 994
AlwI GGATC 4 cut(s) 536, 955, 1010, 1144
AlwNI CAGNNNCTG 1 cut(s) 277
AoxI GGCC 4 cut(s) 100, 356, 477, 1048
ApeKI GCWGC 1 cut(s) 440
Asp700I GAANNNNTTC 1 cut(s) 171
AspA2I CCTAGG 1 cut(s) 1006
AspS9I GGNCC 4 cut(s) 7, 457, 557, 787
AsuC2I CCSGG 3 cut(s) 112, 189, 360
AsuHPI GGTGA 2 cut(s) 401, 437
AsuII TTCGAA 1 cut(s) 696
AvaII GGWCC 4 cut(s) 7, 457, 557, 787
AvrII CCTAGG 1 cut(s) 1006
AxyI CCTNAGG 2 cut(s) 381, 411
BaeI ACNNNNGTAYC 2 cut(s) 881, 914
BbvI GCAGC 1 cut(s) 427
BccI CCATC 7 cut(s) 154, 192, 384, 649, 778, 841, 952
BceAI ACGGC 2 cut(s) 464, 1063
BciT130I CCWGG 3 cut(s) 22, 561, 780
BcnI CCSGG 3 cut(s) 112, 189, 360
BfaI CTAG 1 cut(s) 1007
BfmI CTRYAG 3 cut(s) 90, 238, 735
BisI GCNGC 2 cut(s) 108, 441
BlnI CCTAGG 1 cut(s) 1006
BlsI GCNGC 2 cut(s) 109, 442
Bme1390I CCNGG 6 cut(s) 22, 112, 189, 360, 561, 780
Bme18I GGWCC 4 cut(s) 7, 457, 557, 787
BmgT120I GGNCC 4 cut(s) 7, 457, 557, 787
BmiI GGNNCC 2 cut(s) 558, 789
BmrFI CCNGG 6 cut(s) 22, 112, 189, 360, 561, 780
BmsI GCATC 5 cut(s) 253, 564, 644, 749, 1078
BplI GAGNNNNNCTC 2 cut(s) 1143, 1175
Bpu14I TTCGAA 1 cut(s) 696
BpuMI CCSGG 3 cut(s) 112, 189, 360
Bsa29I ATCGAT 1 cut(s) 1168
BsaBI GATNNNNATC 1 cut(s) 174
BsaJI CCNNGG 6 cut(s) 353, 463, 568, 600, 779, 1006
BsaWI WCCGGW 2 cut(s) 138, 163
Bsc4I CCNNNNNNNGG 5 cut(s) 359, 382, 386, 466, 1046
Bse118I RCCGGY 2 cut(s) 215, 401
Bse1I ACTGG 2 cut(s) 81, 427
Bse21I CCTNAGG 2 cut(s) 381, 411
Bse8I GATNNNNATC 1 cut(s) 174
BseBI CCWGG 3 cut(s) 22, 561, 780
BseCI ATCGAT 1 cut(s) 1168
BseDI CCNNGG 6 cut(s) 353, 463, 568, 600, 779, 1006
BseGI GGATG 6 cut(s) 81, 146, 376, 579, 789, 852
BseJI GATNNNNATC 1 cut(s) 174
BseLI CCNNNNNNNGG 5 cut(s) 359, 382, 386, 466, 1046
BseMII CTCAG 2 cut(s) 395, 558
BseNI ACTGG 2 cut(s) 81, 427
BseRI GAGGAG 1 cut(s) 314
BseXI GCAGC 1 cut(s) 427
BshFI GGCC 4 cut(s) 102, 358, 479, 1050
BshVI ATCGAT 1 cut(s) 1168
BsiSI CCGG 7 cut(s) 112, 139, 164, 188, 216, 359, 402
BslFI GGGAC 2 cut(s) 140, 482
BslI CCNNNNNNNGG 5 cut(s) 359, 382, 386, 466, 1046
BsmFI GGGAC 2 cut(s) 140, 482
BsnI GGCC 4 cut(s) 102, 358, 479, 1050
Bsp119I TTCGAA 1 cut(s) 696
Bsp1407I TGTACA 2 cut(s) 831, 1116
Bsp143I GATC 8 cut(s) 528, 547, 947, 1015, 1124, 1136, 1165, 1169
Bsp19I CCATGG 1 cut(s) 600
BspACI CCGC 7 cut(s) 5, 108, 121, 203, 455, 489, 905
BspANI GGCC 4 cut(s) 102, 358, 479, 1050
BspCNI CTCAG 2 cut(s) 394, 557
BspDI ATCGAT 1 cut(s) 1168
BspLI GGNNCC 2 cut(s) 558, 789
BspPI GGATC 4 cut(s) 536, 955, 1010, 1144
BspT104I TTCGAA 1 cut(s) 696
BsrBI CCGCTC 1 cut(s) 121
BsrFI RCCGGY 2 cut(s) 215, 401
BsrGI TGTACA 2 cut(s) 831, 1116
BsrI ACTGG 2 cut(s) 81, 427
BssAI RCCGGY 2 cut(s) 215, 401
BssECI CCNNGG 6 cut(s) 353, 463, 568, 600, 779, 1006
BssMI GATC 8 cut(s) 528, 547, 947, 1015, 1124, 1136, 1165, 1169
BssT1I CCWWGG 4 cut(s) 463, 568, 600, 1006
Bst2UI CCWGG 3 cut(s) 22, 561, 780
Bst4CI ACNGT 3 cut(s) 46, 836, 897
BstAPI GCANNNNNTGC 1 cut(s) 746
BstAUI TGTACA 2 cut(s) 831, 1116
BstBI TTCGAA 1 cut(s) 696
BstC8I GCNNGC 2 cut(s) 403, 407
BstDEI CTNAG 3 cut(s) 381, 411, 544
BstDSI CCRYGG 1 cut(s) 600
BstF5I GGATG 6 cut(s) 81, 146, 376, 579, 789, 852
BstKTI GATC 8 cut(s) 531, 550, 950, 1018, 1127, 1139, 1168, 1172
BstMBI GATC 8 cut(s) 528, 547, 947, 1015, 1124, 1136, 1165, 1169
BstMWI GCNNNNNNNGC 2 cut(s) 583, 746
BstNI CCWGG 3 cut(s) 22, 561, 780
BstNSI RCATGY 4 cut(s) 586, 635, 956, 1108
BstSCI CCNGG 6 cut(s) 20, 110, 187, 358, 559, 778
BstSFI CTRYAG 3 cut(s) 90, 238, 735
BstV1I GCAGC 1 cut(s) 427
BstX2I RGATCY 2 cut(s) 528, 1136
BstYI RGATCY 2 cut(s) 528, 1136
Bsu15I ATCGAT 1 cut(s) 1168
Bsu36I CCTNAGG 2 cut(s) 381, 411
BsuRI GGCC 4 cut(s) 102, 358, 479, 1050
BsuTUI ATCGAT 1 cut(s) 1168
BtgI CCRYGG 1 cut(s) 600
BtsCI GGATG 6 cut(s) 81, 146, 376, 579, 789, 852
BtsIMutI CAGTG 1 cut(s) 420
Cac8I GCNNGC 2 cut(s) 403, 407
CaiI CAGNNNCTG 1 cut(s) 277
Cfr10I RCCGGY 2 cut(s) 215, 401
Cfr13I GGNCC 4 cut(s) 7, 457, 557, 787
ClaI ATCGAT 1 cut(s) 1168
Csp6I GTAC 6 cut(s) 74, 192, 322, 832, 1003, 1117
CviAII CATG 6 cut(s) 551, 583, 601, 632, 953, 1105
CviQI GTAC 6 cut(s) 74, 192, 322, 832, 1003, 1117
DdeI CTNAG 3 cut(s) 381, 411, 544
DpnI GATC 8 cut(s) 530, 549, 949, 1017, 1126, 1138, 1167, 1171
DpnII GATC 8 cut(s) 528, 547, 947, 1015, 1124, 1136, 1165, 1169
EaeI YGGCCR 1 cut(s) 1048
EciI GGCGGA 1 cut(s) 504
Eco130I CCWWGG 4 cut(s) 463, 568, 600, 1006
Eco32I GATATC 1 cut(s) 57
Eco47I GGWCC 4 cut(s) 7, 457, 557, 787
Eco57I CTGAAG 1 cut(s) 314
Eco81I CCTNAGG 2 cut(s) 381, 411
EcoO109I RGGNCCY 1 cut(s) 557
EcoRII CCWGG 3 cut(s) 20, 559, 778
EcoRV GATATC 1 cut(s) 57
EcoT14I CCWWGG 4 cut(s) 463, 568, 600, 1006
EcoT22I ATGCAT 1 cut(s) 637
ErhI CCWWGG 4 cut(s) 463, 568, 600, 1006
FaeI CATG 6 cut(s) 554, 586, 604, 635, 956, 1108
FaqI GGGAC 2 cut(s) 140, 482
FatI CATG 6 cut(s) 550, 582, 600, 631, 952, 1104
Fnu4HI GCNGC 2 cut(s) 108, 441
FokI GGATG 6 cut(s) 68, 133, 363, 586, 796, 859
Fsp4HI GCNGC 2 cut(s) 108, 441
FspBI CTAG 1 cut(s) 1007
GluI GCNGC 2 cut(s) 108, 441
HaeIII GGCC 4 cut(s) 102, 358, 479, 1050
HapII CCGG 7 cut(s) 112, 139, 164, 188, 216, 359, 402
Hin1II CATG 6 cut(s) 554, 586, 604, 635, 956, 1108
HincII GTYRAC 1 cut(s) 1078
HindII GTYRAC 1 cut(s) 1078
HindIII AAGCTT 1 cut(s) 672
HinfI GANTC 3 cut(s) 128, 135, 349
HpaII CCGG 7 cut(s) 112, 139, 164, 188, 216, 359, 402
HphI GGTGA 2 cut(s) 401, 437
Hpy166II GTNNAC 2 cut(s) 1030, 1078
Hpy188I TCNGA 5 cut(s) 151, 354, 547, 873, 1129
Hpy188III TCNNGA 2 cut(s) 132, 1140
Hpy8I GTNNAC 2 cut(s) 1030, 1078
Hpy99I CGWCG 1 cut(s) 329
HpyAV CCTTC 4 cut(s) 148, 289, 493, 692
HpyCH4III ACNGT 3 cut(s) 46, 836, 897
HpyCH4IV ACGT 1 cut(s) 1132
HpyCH4V TGCA 3 cut(s) 635, 749, 1091
HpyF10VI GCNNNNNNNGC 2 cut(s) 583, 746
HpyF3I CTNAG 3 cut(s) 381, 411, 544
HpySE526I ACGT 1 cut(s) 1132
Hsp92II CATG 6 cut(s) 554, 586, 604, 635, 956, 1108
KroI GCCGGC 1 cut(s) 401
KroNI GCCGGC 1 cut(s) 403
Kzo9I GATC 8 cut(s) 528, 547, 947, 1015, 1124, 1136, 1165, 1169
LmnI GCTCC 4 cut(s) 118, 642, 687, 969
Lsp1109I GCAGC 1 cut(s) 427
LweI GCATC 5 cut(s) 253, 564, 644, 749, 1078
MaeI CTAG 1 cut(s) 1007
MaeII ACGT 1 cut(s) 1132
MaeIII GTNAC 4 cut(s) 35, 273, 433, 730
MalI GATC 8 cut(s) 530, 549, 949, 1017, 1126, 1138, 1167, 1171
MbiI CCGCTC 1 cut(s) 121
MboI GATC 8 cut(s) 528, 547, 947, 1015, 1124, 1136, 1165, 1169
MboII GAAGA 4 cut(s) 179, 634, 637, 863
MfeI CAATTG 1 cut(s) 885
MflI RGATCY 2 cut(s) 528, 1136
MluCI AATT 4 cut(s) 885, 925, 934, 1052
MlyI GAGTC 1 cut(s) 122
MmeI TCCRAC 2 cut(s) 34, 96
Mph1103I ATGCAT 1 cut(s) 637
MroNI GCCGGC 1 cut(s) 401
MroXI GAANNNNTTC 1 cut(s) 171
MslI CAYNNNNRTG 1 cut(s) 728
MspI CCGG 7 cut(s) 112, 139, 164, 188, 216, 359, 402
MspR9I CCNGG 6 cut(s) 22, 112, 189, 360, 561, 780
MunI CAATTG 1 cut(s) 885
MvaI CCWGG 3 cut(s) 22, 561, 780
MwoI GCNNNNNNNGC 2 cut(s) 583, 746
NaeI GCCGGC 1 cut(s) 403
NciI CCSGG 3 cut(s) 112, 189, 360
NcoI CCATGG 1 cut(s) 600
NdeII GATC 8 cut(s) 528, 547, 947, 1015, 1124, 1136, 1165, 1169
NgoMIV GCCGGC 1 cut(s) 401
NlaIII CATG 6 cut(s) 554, 586, 604, 635, 956, 1108
NlaIV GGNNCC 2 cut(s) 558, 789
NmeAIII GCCGAG 1 cut(s) 128
NmuCI GTSAC 1 cut(s) 730
NsiI ATGCAT 1 cut(s) 637
NspI RCATGY 4 cut(s) 586, 635, 956, 1108
NspV TTCGAA 1 cut(s) 696
PciI ACATGT 1 cut(s) 1104
PcsI WCGNNNNNNNCGW 1 cut(s) 56
PdiI GCCGGC 1 cut(s) 403
PdmI GAANNNNTTC 1 cut(s) 171
PfeI GAWTC 2 cut(s) 135, 349
PkrI GCNGC 2 cut(s) 109, 442
PleI GAGTC 1 cut(s) 122
PpsI GAGTC 1 cut(s) 122
PpuMI RGGWCCY 1 cut(s) 557
PscI ACATGT 1 cut(s) 1104
Psp5II RGGWCCY 1 cut(s) 557
Psp6I CCWGG 3 cut(s) 20, 559, 778
PspGI CCWGG 3 cut(s) 20, 559, 778
PspN4I GGNNCC 2 cut(s) 558, 789
PspPI GGNCC 4 cut(s) 7, 457, 557, 787
PspPPI RGGWCCY 1 cut(s) 557
PstNI CAGNNNCTG 1 cut(s) 277
PsuI RGATCY 2 cut(s) 528, 1136
RsaI GTAC 6 cut(s) 75, 193, 323, 833, 1004, 1118
RsaNI GTAC 6 cut(s) 74, 192, 322, 832, 1003, 1117
RseI CAYNNNNRTG 1 cut(s) 728
SatI GCNGC 2 cut(s) 108, 441
Sau3AI GATC 8 cut(s) 528, 547, 947, 1015, 1124, 1136, 1165, 1169
Sau96I GGNCC 4 cut(s) 7, 457, 557, 787
SchI GAGTC 1 cut(s) 122
ScrFI CCNGG 6 cut(s) 22, 112, 189, 360, 561, 780
SfaNI GCATC 5 cut(s) 253, 564, 644, 749, 1078
SfcI CTRYAG 3 cut(s) 90, 238, 735
SfuI TTCGAA 1 cut(s) 696
SinI GGWCC 4 cut(s) 7, 457, 557, 787
SmiMI CAYNNNNRTG 1 cut(s) 728
Sse9I AATT 4 cut(s) 885, 925, 934, 1052
SsiI CCGC 7 cut(s) 5, 108, 121, 203, 455, 489, 905
SspMI CTAG 1 cut(s) 1007
StyD4I CCNGG 6 cut(s) 20, 110, 187, 358, 559, 778
StyI CCWWGG 4 cut(s) 463, 568, 600, 1006
TaaI ACNGT 3 cut(s) 46, 836, 897
TaiI ACGT 1 cut(s) 1135
TaqI TCGA 2 cut(s) 696, 1168
TasI AATT 4 cut(s) 885, 925, 934, 1052
TatI WGTACW 3 cut(s) 73, 831, 1116
TauI GCSGC 1 cut(s) 110
TfiI GAWTC 2 cut(s) 135, 349
TscAI CASTG 1 cut(s) 427
TseFI GTSAC 1 cut(s) 730
TseI GCWGC 1 cut(s) 440
Tsp45I GTSAC 1 cut(s) 730
TspDTI ATGAA 2 cut(s) 164, 362
TspGWI ACGGA 1 cut(s) 931
TspRI CASTG 1 cut(s) 427
VpaK11BI GGWCC 4 cut(s) 7, 457, 557, 787
XceI RCATGY 4 cut(s) 586, 635, 956, 1108
XcmI CCANNNNNNNNNTGG 2 cut(s) 92, 383
XmaJI CCTAGG 1 cut(s) 1006
XmnI GAANNNNTTC 1 cut(s) 171
XspI CTAG 1 cut(s) 1007
Zsp2I ATGCAT 1 cut(s) 637
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.