pycom04g15660

Zinc transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0011912
Physical Location & Seq
Forward (+)
18479685 .. 18482371
2687 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1791 bp
ATGACCTTCACATTTAGAAAAGTTCTATTATTTTCGCTGTTCTCCCTAGTTCTCTTTGGGTACACTACATCAGAGATTGAAAATGAAGTGACGCAAAAGCTGAGATCTGCTCCACACAAAAATGTGAGAATTAATGTTATTGATGGCACGGGTCAGGAGAATGCCATAAATTTTGAGGTTTCAAATAATGGACTGGGTGAGGTGAAGAGCGCAAACAGCAAAGTCTCAGTCTCTACTGTTGCGTTATTTACGTTGGCAATGGCTGCTGCCACAGGTTTAGGTGCAGTTCCATTCTTCTTTGTTGAGCTTGATCCACAATGGGCGGGATTATGCAATGGGATGGCTGCTGGTGTAATGTTGGCTGCAAGCTTTGATCTTGTACAGGAAGGACAAGGCCATGGTGCTGGGAACTGGGTTGTTGTTGGGCTTTTAGCTGGGGGCCTTTTTATTTGGCTATGCAAGCAGTTTCTTGAGCAATACGGAGAAGTTAGTATGTTAGACATCAAAGGTGCAGATGCAACTAAAGTTGTTCTTGTGATTGGAATAATGACTCTTCATTCATTTGGGGAGGGCTCTGGTGTTGGGGTTTCCTTTGCTGGCTCAAAGGGTTTCACTCAGGGCCTTTTGGTTACTTTGGCTATTGCTGTGCACAATATACCAGAAGGCTTAGCTGTGAGTATGGTGCTTGCATCCAGGGGTGTTTCTCCGCAGAATGCCATGTTATGGAGCATAATTACATCATTACCACAGCCCCTTGTAGCAGTTCCTTCATTCATATGCGCTGATGCATTTAGCAAGTTCCTTCCTTTCTGTACGGGCTTTGCTGCTGGATGTATGATCTGGATGGTTGTTGCGGAGGTACTTCCTGATGCATTTAAGGAAGCCTCTCCATCACAGGTTGCGTCAGCAGCTACTCTCTCTGTTGCATTCATGGAAGCTCTTAGCACTCTTTTTCAGAATTTCACCCATGATTACAACTCAGAGGATGCTTCTGGCTTCTTTGTGTCATTACTTTTTGGTCTTGGACCATTGCTCGGTGGCACTGTTCTTGTCGCCTTTGCATTAGCTTTCCATCTTCAGCATGCTCTTCTGATGGGTGCAGCATCTGGCATTGCCTTTGTCCTTGGTGCCTGGACACCATTGCAGCTACTTTTTTCATTAAAGATGGGATTTCTATCTGCTATATTTCTGCTTGCAACGGGAGCTGGATCCGTCCATATTTTGAGCTCTAGTATTCTGAAGCTCGCAGGTCGCAAAAAAACTTCGGTTCATAACTTACCCACGGCAAATGGTTTTCCAGTGAGCATTCACACCCTCCAGGCATTCTTGTCATGTGGAGCAGTTGCCTTTCATGCTTTGGCCGAAGGGCTTGCACTAGGAGTTGCTGCACCAAAAGCCTACGGACTCGGGCGACACATGGTCCTTCCTGTCTCCCTACATGGACTCCCTCGGGGTGCAGCTGTGGCTAGCTGCATATTTGGGGCTACTGATAACTGGCATGCAGCCCTTGTGGCGGCTGCTTTAATTGGATTCGTGGGTCCAATATCTGCAATAGGAGCAATACTGGCAGGAATTGATTATAGCGGATTAGACCATGTGATGGTTTTTGCTTGTGGGGGACTGCTCCCGAGTTTTGCAAAAGTAGTGAAGAGAGCAGTGAAGTTGGATACACGTAAAAGTAGCTGTGGGATTCTGATTGGTGTGGGGTTTGCAACTCTGTGTCTGACTTGCACGAAGTTGGTGTGCTTGCACACACCTTATTGCAATTCTGCACCAGAGGCTGTCAGATGA

Protein Analysis

597

Amino Acids

62.0

Weight (kDa)

7.23

Isoelectric Point (pI)

29.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zip PF02535 173 - 295 7.2e-12 ZIP Zinc transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011912)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G08650 AT3G08650
fragaria_vesca FvH4_6g09450 FvH4_6g09450
malus_domestica MD04G1177600.v1.1 MD12G1192900.v1.1
prunus_persica Prupe.6G297500_v2.0.a1
pyrus_communis pycom04g15660 pycom12g17960
rosa_chinensis RchiOBHm_Chr3g0459921
rosa_laevigata RLG00000025051
rosa_multiflora Rmu_sc0000240.1_g000047 Rmu_sc0016534.1_g000002
rosa_roxburghii Rroxscaffold_6G00419910
rosa_rugosa Rorug03G0038800
rosa_samantha Rh3AG097400 Rh3BG100300 Rh3CG102100 Rh3DG101800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1238
AccB1I GGYRCC 1 cut(s) 1127
AccB7I CCANNNNNTGG 2 cut(s) 723, 1600
AciI CCGC 5 cut(s) 323, 707, 854, 1514, 1584
AclWI GGATC 3 cut(s) 305, 1203, 1216
AcoI YGGCCR 1 cut(s) 1359
AcsI RAATTY 2 cut(s) 169, 958
AcuI CTGAAG 2 cut(s) 1061, 1259
AfaI GTAC 4 cut(s) 62, 381, 814, 861
AfiI CCNNNNNNNGG 4 cut(s) 723, 1034, 1513, 1600
AflIII ACRYGT 1 cut(s) 1670
AgsI TTSAA 2 cut(s) 80, 183
AhdI GACNNNNNGTC 1 cut(s) 1418
AjnI CCWGG 3 cut(s) 692, 1130, 1317
Alw21I GWGCWC 2 cut(s) 651, 1229
Alw26I GTCTC 3 cut(s) 229, 235, 1435
Alw44I GTGCAC 1 cut(s) 647
AlwI GGATC 3 cut(s) 305, 1203, 1216
AlwNI CAGNNNCTG 2 cut(s) 1106, 1781
Ama87I CYCGRG 3 cut(s) 1406, 1449, 1627
AoxI GGCC 4 cut(s) 394, 439, 619, 1359
ApaLI GTGCAC 1 cut(s) 647
ApoI RAATTY 2 cut(s) 169, 958
ArsI GACNNNNNNTTYG 2 cut(s) 213, 245
AseI ATTAAT 1 cut(s) 132
AspLEI GCGC 2 cut(s) 212, 782
AspS9I GGNCC 5 cut(s) 439, 619, 1025, 1420, 1538
AsuHPI GGTGA 3 cut(s) 209, 214, 955
AsuNHI GCTAGC 1 cut(s) 1466
AvaI CYCGRG 3 cut(s) 1406, 1449, 1627
AvaII GGWCC 3 cut(s) 1025, 1420, 1538
BaeGI GKGCMC 1 cut(s) 651
BamHI GGATCC 1 cut(s) 1208
BanI GGYRCC 1 cut(s) 1127
BanII GRGCYC 2 cut(s) 575, 1229
Bbv12I GWGCWC 2 cut(s) 651, 1229
BccI CCATC 8 cut(s) 137, 334, 838, 898, 1080, 1087, 1159, 1594
BceAI ACGGC 1 cut(s) 1299
BcgI CGANNNNNNTGC 2 cut(s) 1352, 1386
BciT130I CCWGG 3 cut(s) 694, 1132, 1319
BciVI GTATCC 1 cut(s) 1660
BcoDI GTCTC 3 cut(s) 229, 235, 1435
BfaI CTAG 4 cut(s) 47, 1230, 1376, 1467
BfuAI ACCTGC 1 cut(s) 1238
BfuI GTATCC 1 cut(s) 1660
BglI GCCNNNNNGGC 1 cut(s) 1511
BglII AGATCT 1 cut(s) 104
BlpI GCTNAGC 1 cut(s) 667
Bme1390I CCNGG 3 cut(s) 694, 1132, 1319
Bme18I GGWCC 3 cut(s) 1025, 1420, 1538
BmeRI GACNNNNNGTC 1 cut(s) 1418
BmeT110I CYCGRG 3 cut(s) 1406, 1449, 1627
BmgT120I GGNCC 5 cut(s) 439, 619, 1025, 1420, 1538
BmiI GGNNCC 4 cut(s) 440, 1129, 1210, 1539
BmrFI CCNGG 3 cut(s) 694, 1132, 1319
BmrI ACTGGG 2 cut(s) 203, 421
BmsI GCATC 6 cut(s) 505, 698, 775, 859, 976, 1112
BmtI GCTAGC 1 cut(s) 1470
BmuI ACTGGG 2 cut(s) 203, 421
BplI GAGNNNNNCTC 2 cut(s) 94, 126
BpmI CTGGAG 1 cut(s) 1301
Bpu1102I GCTNAGC 1 cut(s) 667
BpuEI CTTGAG 1 cut(s) 491
BsaAI YACGTR 1 cut(s) 1673
BsaBI GATNNNNATC 1 cut(s) 1174
BsaJI CCNNGG 5 cut(s) 397, 693, 1123, 1281, 1448
BsaXI ACNNNNNCTCC 2 cut(s) 1428, 1458
Bsc4I CCNNNNNNNGG 4 cut(s) 723, 1034, 1513, 1600
Bse1I ACTGG 5 cut(s) 198, 416, 1298, 1499, 1569
Bse3DI GCAATG 5 cut(s) 264, 340, 1028, 1110, 1139
Bse8I GATNNNNATC 1 cut(s) 1174
BseBI CCWGG 3 cut(s) 694, 1132, 1319
BseDI CCNNGG 5 cut(s) 397, 693, 1123, 1281, 1448
BseGI GGATG 5 cut(s) 345, 689, 836, 849, 991
BseJI GATNNNNATC 1 cut(s) 1174
BseLI CCNNNNNNNGG 4 cut(s) 723, 1034, 1513, 1600
BseMI GCAATG 5 cut(s) 264, 340, 1028, 1110, 1139
BseMII CTCAG 4 cut(s) 92, 240, 629, 993
BseNI ACTGG 5 cut(s) 198, 416, 1298, 1499, 1569
BseSI GKGCMC 1 cut(s) 651
BseYI CCCAGC 2 cut(s) 404, 434
BsgI GTGCAG 6 cut(s) 303, 531, 1119, 1371, 1476, 1755
BshFI GGCC 4 cut(s) 396, 441, 621, 1361
BshNI GGYRCC 1 cut(s) 1127
BsiHKAI GWGCWC 2 cut(s) 651, 1229
BsiHKCI CYCGRG 3 cut(s) 1406, 1449, 1627
BslFI GGGAC 1 cut(s) 1632
BslI CCNNNNNNNGG 4 cut(s) 723, 1034, 1513, 1600
BsmAI GTCTC 3 cut(s) 229, 235, 1435
BsmFI GGGAC 1 cut(s) 1632
BsmI GAATGC 5 cut(s) 166, 718, 926, 1305, 1322
BsnI GGCC 4 cut(s) 396, 441, 621, 1361
BsoBI CYCGRG 3 cut(s) 1406, 1449, 1627
Bsp1286I GDGCHC 3 cut(s) 575, 651, 1229
Bsp1407I TGTACA 1 cut(s) 379
Bsp143I GATC 5 cut(s) 104, 310, 373, 837, 1208
Bsp1720I GCTNAGC 1 cut(s) 667
Bsp19I CCATGG 1 cut(s) 397
BspACI CCGC 5 cut(s) 323, 707, 854, 1514, 1584
BspANI GGCC 4 cut(s) 396, 441, 621, 1361
BspCNI CTCAG 4 cut(s) 93, 239, 628, 992
BspLI GGNNCC 4 cut(s) 440, 1129, 1210, 1539
BspMI ACCTGC 1 cut(s) 1238
BspOI GCTAGC 1 cut(s) 1470
BspPI GGATC 3 cut(s) 305, 1203, 1216
BspQI GCTCTTC 2 cut(s) 200, 1092
BspT107I GGYRCC 1 cut(s) 1127
BsrDI GCAATG 5 cut(s) 264, 340, 1028, 1110, 1139
BsrGI TGTACA 1 cut(s) 379
BsrI ACTGG 5 cut(s) 198, 416, 1298, 1499, 1569
BssECI CCNNGG 5 cut(s) 397, 693, 1123, 1281, 1448
BssMI GATC 5 cut(s) 104, 310, 373, 837, 1208
BssT1I CCWWGG 2 cut(s) 397, 1123
Bst2UI CCWGG 3 cut(s) 694, 1132, 1319
Bst4CI ACNGT 2 cut(s) 238, 1045
Bst6I CTCTTC 4 cut(s) 200, 558, 1092, 1643
BstAPI GCANNNNNTGC 1 cut(s) 263
BstAUI TGTACA 1 cut(s) 379
BstBAI YACGTR 1 cut(s) 1673
BstDEI CTNAG 6 cut(s) 101, 226, 615, 667, 941, 979
BstDSI CCRYGG 2 cut(s) 397, 1281
BstF5I GGATG 5 cut(s) 345, 689, 836, 849, 991
BstHHI GCGC 2 cut(s) 212, 782
BstKTI GATC 5 cut(s) 107, 313, 376, 840, 1211
BstMAI GTCTC 3 cut(s) 229, 235, 1435
BstMBI GATC 5 cut(s) 104, 310, 373, 837, 1208
BstNI CCWGG 3 cut(s) 694, 1132, 1319
BstNSI RCATGY 2 cut(s) 1085, 1502
BstSCI CCNGG 3 cut(s) 692, 1130, 1317
BstSLI GKGCMC 1 cut(s) 651
BstX2I RGATCY 2 cut(s) 104, 1208
BstXI CCANNNNNNTGG 1 cut(s) 404
BstYI RGATCY 2 cut(s) 104, 1208
BsuI GTATCC 1 cut(s) 1660
BsuRI GGCC 4 cut(s) 396, 441, 621, 1361
BtgI CCRYGG 2 cut(s) 397, 1281
BtsCI GGATG 5 cut(s) 345, 689, 836, 849, 991
BtsI GCAGTG 1 cut(s) 1662
BtsIMutI CAGTG 3 cut(s) 1041, 1305, 1662
BveI ACCTGC 1 cut(s) 1238
CaiI CAGNNNCTG 2 cut(s) 1106, 1781
CfoI GCGC 2 cut(s) 212, 782
Cfr13I GGNCC 5 cut(s) 439, 619, 1025, 1420, 1538
CseI GACGC 2 cut(s) 100, 891
Csp6I GTAC 4 cut(s) 61, 380, 813, 860
CviQI GTAC 4 cut(s) 61, 380, 813, 860
DdeI CTNAG 6 cut(s) 101, 226, 615, 667, 941, 979
DpnI GATC 5 cut(s) 106, 312, 375, 839, 1210
DpnII GATC 5 cut(s) 104, 310, 373, 837, 1208
DriI GACNNNNNGTC 1 cut(s) 1418
EaeI YGGCCR 1 cut(s) 1359
Eam1104I CTCTTC 4 cut(s) 200, 558, 1092, 1643
Eam1105I GACNNNNNGTC 1 cut(s) 1418
EarI CTCTTC 4 cut(s) 200, 558, 1092, 1643
Ecl136II GAGCTC 1 cut(s) 1227
Eco130I CCWWGG 2 cut(s) 397, 1123
Eco24I GRGCYC 2 cut(s) 575, 1229
Eco47I GGWCC 3 cut(s) 1025, 1420, 1538
Eco53kI GAGCTC 1 cut(s) 1227
Eco57I CTGAAG 2 cut(s) 1061, 1259
Eco88I CYCGRG 3 cut(s) 1406, 1449, 1627
EcoICRI GAGCTC 1 cut(s) 1227
EcoO109I RGGNCCY 2 cut(s) 439, 619
EcoRII CCWGG 3 cut(s) 692, 1130, 1317
EcoT14I CCWWGG 2 cut(s) 397, 1123
EcoT22I ATGCAT 2 cut(s) 790, 874
EcoT38I GRGCYC 2 cut(s) 575, 1229
ErhI CCWWGG 2 cut(s) 397, 1123
FalI AAGNNNNNCTT 2 cut(s) 516, 548
FaqI GGGAC 1 cut(s) 1632
FauI CCCGC 1 cut(s) 316
FauNDI CATATG 1 cut(s) 776
FokI GGATG 5 cut(s) 352, 676, 843, 856, 998
FriOI GRGCYC 2 cut(s) 575, 1229
FspBI CTAG 4 cut(s) 47, 1230, 1376, 1467
GlaI GCGC 2 cut(s) 211, 781
GsaI CCCAGC 2 cut(s) 408, 438
GsuI CTGGAG 1 cut(s) 1301
HaeIII GGCC 4 cut(s) 396, 441, 621, 1361
HgaI GACGC 2 cut(s) 100, 891
HhaI GCGC 2 cut(s) 212, 782
Hin6I GCGC 2 cut(s) 210, 780
HinP1I GCGC 2 cut(s) 210, 780
HindIII AAGCTT 1 cut(s) 367
HinfI GANTC 5 cut(s) 550, 1404, 1443, 1530, 1690
HphI GGTGA 3 cut(s) 209, 214, 955
Hpy166II GTNNAC 2 cut(s) 63, 649
Hpy188I TCNGA 8 cut(s) 73, 957, 982, 1092, 1239, 1695, 1725, 1787
Hpy188III TCNNGA 5 cut(s) 155, 470, 841, 866, 1627
Hpy8I GTNNAC 2 cut(s) 63, 649
HpyAV CCTTC 7 cut(s) 16, 380, 656, 777, 812, 1358, 1433
HpyCH4III ACNGT 2 cut(s) 238, 1045
HpyCH4IV ACGT 2 cut(s) 251, 1672
HpyF3I CTNAG 6 cut(s) 101, 226, 615, 667, 941, 979
HpySE526I ACGT 2 cut(s) 251, 1672
HspAI GCGC 2 cut(s) 210, 780
Kzo9I GATC 5 cut(s) 104, 310, 373, 837, 1208
LguI GCTCTTC 2 cut(s) 200, 1092
LmnI GCTCC 6 cut(s) 115, 726, 1202, 1337, 1556, 1629
LweI GCATC 6 cut(s) 505, 698, 775, 859, 976, 1112
MaeI CTAG 4 cut(s) 47, 1230, 1376, 1467
MaeII ACGT 2 cut(s) 251, 1672
MaeIII GTNAC 2 cut(s) 88, 628
MalI GATC 5 cut(s) 106, 312, 375, 839, 1210
MboI GATC 5 cut(s) 104, 310, 373, 837, 1208
MboII GAAGA 6 cut(s) 217, 286, 545, 1067, 1079, 1660
MflI RGATCY 2 cut(s) 104, 1208
MhlI GDGCHC 3 cut(s) 575, 651, 1229
MluCI AATT 7 cut(s) 129, 169, 732, 958, 1524, 1572, 1765
MlyI GAGTC 3 cut(s) 544, 1398, 1437
MmeI TCCRAC 1 cut(s) 1644
MnlI CCTC 9 cut(s) 169, 193, 562, 850, 895, 976, 1325, 1458, 1771
Mph1103I ATGCAT 2 cut(s) 790, 874
MseI TTAA 4 cut(s) 132, 876, 1160, 1523
MslI CAYNNNNRTG 2 cut(s) 120, 775
MspA1I CMGCKG 1 cut(s) 1460
MspR9I CCNGG 3 cut(s) 694, 1132, 1319
Mva1269I GAATGC 5 cut(s) 166, 718, 926, 1305, 1322
MvaI CCWGG 3 cut(s) 694, 1132, 1319
NcoI CCATGG 1 cut(s) 397
NdeI CATATG 1 cut(s) 776
NdeII GATC 5 cut(s) 104, 310, 373, 837, 1208
NheI GCTAGC 1 cut(s) 1466
NlaIV GGNNCC 4 cut(s) 440, 1129, 1210, 1539
NmuCI GTSAC 1 cut(s) 88
NsiI ATGCAT 2 cut(s) 790, 874
NspI RCATGY 2 cut(s) 1085, 1502
PaeI GCATGC 2 cut(s) 1085, 1502
PciSI GCTCTTC 2 cut(s) 200, 1092
PctI GAATGC 5 cut(s) 166, 718, 926, 1305, 1322
PfeI GAWTC 2 cut(s) 1530, 1690
PflMI CCANNNNNTGG 2 cut(s) 723, 1600
PleI GAGTC 3 cut(s) 544, 1398, 1437
PpsI GAGTC 3 cut(s) 544, 1398, 1437
Ppu21I YACGTR 1 cut(s) 1673
PshBI ATTAAT 1 cut(s) 132
Psp124BI GAGCTC 1 cut(s) 1229
Psp6I CCWGG 3 cut(s) 692, 1130, 1317
PspFI CCCAGC 2 cut(s) 404, 434
PspGI CCWGG 3 cut(s) 692, 1130, 1317
PspN4I GGNNCC 4 cut(s) 440, 1129, 1210, 1539
PspPI GGNCC 5 cut(s) 439, 619, 1025, 1420, 1538
PstNI CAGNNNCTG 2 cut(s) 1106, 1781
PsuI RGATCY 2 cut(s) 104, 1208
PvuII CAGCTG 1 cut(s) 1460
RsaI GTAC 4 cut(s) 62, 381, 814, 861
RsaNI GTAC 4 cut(s) 61, 380, 813, 860
RseI CAYNNNNRTG 2 cut(s) 120, 775
SacI GAGCTC 1 cut(s) 1229
SapI GCTCTTC 2 cut(s) 200, 1092
SaqAI TTAA 4 cut(s) 132, 876, 1160, 1523
Sau3AI GATC 5 cut(s) 104, 310, 373, 837, 1208
Sau96I GGNCC 5 cut(s) 439, 619, 1025, 1420, 1538
SchI GAGTC 3 cut(s) 544, 1398, 1437
ScrFI CCNGG 3 cut(s) 694, 1132, 1319
SduI GDGCHC 3 cut(s) 575, 651, 1229
SfaNI GCATC 6 cut(s) 505, 698, 775, 859, 976, 1112
SinI GGWCC 3 cut(s) 1025, 1420, 1538
SmiMI CAYNNNNRTG 2 cut(s) 120, 775
SmlI CTYRAG 1 cut(s) 470
SmoI CTYRAG 1 cut(s) 470
SphI GCATGC 2 cut(s) 1085, 1502
Sse9I AATT 7 cut(s) 129, 169, 732, 958, 1524, 1572, 1765
SsiI CCGC 5 cut(s) 323, 707, 854, 1514, 1584
SspMI CTAG 4 cut(s) 47, 1230, 1376, 1467
SstI GAGCTC 1 cut(s) 1229
StyD4I CCNGG 3 cut(s) 692, 1130, 1317
StyI CCWWGG 2 cut(s) 397, 1123
TaaI ACNGT 2 cut(s) 238, 1045
TaiI ACGT 2 cut(s) 254, 1675
TasI AATT 7 cut(s) 129, 169, 732, 958, 1524, 1572, 1765
TatI WGTACW 1 cut(s) 379
TauI GCSGC 1 cut(s) 1517
TfiI GAWTC 2 cut(s) 1530, 1690
Tru1I TTAA 4 cut(s) 132, 876, 1160, 1523
Tru9I TTAA 4 cut(s) 132, 876, 1160, 1523
TscAI CASTG 3 cut(s) 1048, 1305, 1662
TseFI GTSAC 1 cut(s) 88
Tsp45I GTSAC 1 cut(s) 88
TspDTI ATGAA 9 cut(s) 99, 545, 549, 759, 763, 919, 1146, 1259, 1340
TspGWI ACGGA 3 cut(s) 495, 1201, 1416
TspRI CASTG 3 cut(s) 1048, 1305, 1662
Van91I CCANNNNNTGG 2 cut(s) 723, 1600
VneI GTGCAC 1 cut(s) 647
VpaK11BI GGWCC 3 cut(s) 1025, 1420, 1538
VspI ATTAAT 1 cut(s) 132
XapI RAATTY 2 cut(s) 169, 958
XceI RCATGY 2 cut(s) 1085, 1502
XspI CTAG 4 cut(s) 47, 1230, 1376, 1467
Zsp2I ATGCAT 2 cut(s) 790, 874
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.