pycom04g16840

Belongs to the peptidase C1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0019773
Physical Location & Seq
Forward (+)
19427633 .. 19432141
4509 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2178 bp
ATGGCTCTTCTCATCACAAACCAAGGCCAATCCCTCCGCTTGGCCTCATTTCTCATGCTCATTTTGGCCTTCTGCTCCTCGAAAGCACTTTGTAGTCGCCCACTTTATGGCAACAAAATCACGGTTGTCAGGCACGAGGAGTGGATGGCTCAACATGGACGCACTTATAATGATGCACAAGAGAAGGAGATGCGCCACAACATATTCAAGAACAATGTGGAATTTGTACAAGCTTTTAACAAAAACAAGGAGGGAAAAAAGTACACGCTCAGTGTCAATAAGTTTGCGGACAAAACTAACGAGGAAATTCAGCCAATGCGCAATGGCTACAAGAGAAAGCAACGTTCAGGGTTCATGTCCGACTCTTTGATAGCATCTCCTTTTCGATATGGAAATGTCAGTAATGTGCCATCTTCTGTGGATTGGAGATTGTCTGGCGCTGTGACCGCGGTCAAGAACCAAGGATTTGAGTGTGGGTGTTGTTGGGCGTTCGCAACAGTAGCAACCATTGAAGGACTTAACAAACTCAAAACAAGAAACCTAATTTCACTATCAGAACAAGAGCTAGTTGATTGTGACACGAGCAGTTACAATCACGGATGTAATGGCGGTAATTCAAAGGTTGCGTATGACTACATGATCAACCGCAACAGGAGCCTTGTAACTGAAGATAATTACCCTTACCAGGCTAAAGATGGAGGAATTTGCAAAACTGCTAGTGCTGCTCCTGCATCTGGTGCCATAACCATAACCGGCTACGAGAATGTGCCTGCAAACAACGAAGATGCATTGTTGAAGGCTGTTGCGAACCAGCCGGTTTCTGTTGCCATTGATATTGACGCAGAGGAATTCAAGTATTATTCGGGTGGCGTGTACACTGGACCATGTGGTACAAACTTAAGCCATGAAGTGACTGCAGTTGGGTACGGTACTAGTAATGATGGGACCAAGTATTGGCTGCTCAAGAATTCTTGGGGCAAAGATTGGGGAGAGGGTGGATACATGAGATTACAAAGAGACGTGCCCGCCAAGGAAGGGTTATGTGGCATTGCTATGGAAGCTACTTATCCAACCAAAATCCCACGCCATGCTGAATGTGTTGTATACCAAGATAACTTATCTAAAGTACTCCAAGTGCTTACCAATATCATGGCTCTACTCATCACAAACCAAGGCCAATCCCTCGCCTTATTTCTGGTTCTCGTTTTGGGCTTCTGCTCCTCCCAAGCTTTTTGTAGTCGTCAACTTTCTGAGGACGAACAAATCATGTTGGAGAGGCACAAAGAGTGGATGGCTCGACATGGACGTGTTTACAAGGATGCACAAGAGAAAGACAGGCGTTACAGGACATTCAAGTCCAGTGTGGAGTTTATAGAAGGTTTTAACAAGAAGAGCGAAAACAAGTACAGGCTTGACATCAATAAGTTTGTTGACCTAACTAACGAGGAATTTCAGGCGACGAGTCAAGGCTACAAGAGGATACACCTCCCAGAGATCATGTCCAACTCTTCCAAAGCAGCTCCAGATACCAATTTTCGATATGAAAATGTCACCGATGTGCCAAAATCTAAGGATTATTTTTCAATTGGCGCTGTGACCACGGTCAAATACCAAGGGCAATGTGGATGTTGTTGGGCGTTCGCAACAGTAGCAACCATAGAAGGGCTTAACAAACTCAAAACAGGAAACCTAATTTCACTATCAGAACAAGATCAAAGGTTGCGTATGACTACATCGATCAAAAGCAACAAGAGCCTTGCAACTGAAGATAATTACCCTTACCAGGCTAAAGATGGAGGAATTTGCAGAACTGCTGCTGCTGCTCCTGCATCTGGTGCCATAACCATAACCGGCTACGAGAATGTGCCTGCAAACAACGAAGATGCATTGTTGAAGGCTGTTGCGAACCAGCCGGTTTCTGTTGCCATTGATATTAACGCAGAGGAATTCAGGTATTATTCGAGTGGCGTGTACACTGGACCATGCGGTACAAACTTAAGCCATGAAGTGACTGCAGTTGGGTACGGTACTAGTAATGATGGGACTAAGTATTGGCTGCTCAAGAATTCTTGGGGCAAAGAGTGGGGGGAGAGTGGATACATTAGATTACAAAGAGACGTGCCAGCCAAGGAAGGTCTATGTGGCATTGCCATGGAAGCTACCTATCCAACCGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000323 GO:0000325 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005773 GO:0006508 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0008219 GO:0008233 GO:0008234 GO:0009056 GO:0009057 GO:0009314 GO:0009411 GO:0009416 GO:0009605 GO:0009607 GO:0009620 GO:0009626 GO:0009628 GO:0009719 GO:0009725 GO:0009733 GO:0009735 GO:0009743 GO:0009744 GO:0009746 GO:0009749 GO:0009750 GO:0009791 GO:0009814 GO:0009817 GO:0009908 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010150 GO:0010224 GO:0010282 GO:0010623 GO:0012501 GO:0016787 GO:0019538 GO:0022414 GO:0030154 GO:0030163 GO:0032501 GO:0032502 GO:0033554 GO:0034050 GO:0034284 GO:0034285 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044424 GO:0044444 GO:0044464 GO:0045087 GO:0046688 GO:0048366 GO:0048367 GO:0048437 GO:0048468 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050832 GO:0050896 GO:0051603 GO:0051704 GO:0051707 GO:0051716 GO:0061458 GO:0070011 GO:0071704 GO:0080187 GO:0090567 GO:0090693 GO:0097501 GO:0098542 GO:0099402 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1901700 GO:1990169
KEGG Pathways
Metabolic & Signaling
ko04140 ko04142 ko04145 ko04210 ko04612 ko05205 ko05323 ko05418 map04140 map04142 map04145 map04210 map04612 map05205 map05323 map05418
Pfam Domains
Protein Families
Inhibitor_I29 Peptidase_C1

Protein Analysis

726

Amino Acids

80.4

Weight (kDa)

8.1

Isoelectric Point (pI)

35.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I29 PF08246 45 - 103 5.2e-16 Cathepsin propeptide inhibitor domain (I29)
Peptidase_C1 PF00112 136 - 358 7.9e-74 Papain family cysteine protease
Inhibitor_I29 PF08246 427 - 484 4.5e-15 Cathepsin propeptide inhibitor domain (I29)
Peptidase_C1 PF00112 520 - 724 4.2e-54 Papain family cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019773)

Species Orthologous Gene IDs
malus_domestica MD04G1190100.v1.1 MD04G1190200.v1.1 MD12G1202900.v1.1 MD12G1203000.v1.1
prunus_persica Prupe.4G153800_v2.0.a1
pyrus_communis pycom04g16840 pycom12g18940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 168
AasI GACNNNNNNGTC 1 cut(s) 1354
Acc16I TGCGCA 1 cut(s) 320
AccB1I GGYRCC 2 cut(s) 737, 1835
AccB7I CCANNNNNTGG 2 cut(s) 107, 954
AccI GTMKAC 1 cut(s) 1104
AccII CGCG 1 cut(s) 449
AciI CCGC 9 cut(s) 37, 287, 447, 449, 609, 646, 1026, 1986, 2172
AclI AACGTT 1 cut(s) 343
AcsI RAATTY 9 cut(s) 221, 306, 702, 848, 967, 1448, 1800, 1946, 2065
AcuI CTGAAG 2 cut(s) 687, 1785
AfiI CCNNNNNNNGG 9 cut(s) 40, 107, 685, 734, 954, 1035, 1662, 1783, 1832
AflII CTTAAG 2 cut(s) 898, 1996
AflIII ACRYGT 1 cut(s) 1306
AgsI TTSAA 8 cut(s) 208, 512, 618, 796, 853, 1354, 1584, 1894
AhlI ACTAGT 2 cut(s) 932, 2030
AjiI CACGTC 3 cut(s) 1021, 1307, 2119
AjnI CCWGG 2 cut(s) 684, 1782
AleI CACNNNNGTG 1 cut(s) 1556
AluBI AGCT 6 cut(s) 233, 565, 1061, 1229, 1520, 2159
AluI AGCT 6 cut(s) 233, 565, 1061, 1229, 1520, 2159
Alw26I GTCTC 2 cut(s) 1011, 2109
AoxI GGCC 4 cut(s) 25, 42, 66, 1174
ApeKI GCWGC 7 cut(s) 722, 958, 1517, 1814, 1817, 1820, 2056
ApoI RAATTY 9 cut(s) 221, 306, 702, 848, 967, 1448, 1800, 1946, 2065
AspLEI GCGC 4 cut(s) 195, 321, 440, 1592
AspS9I GGNCC 3 cut(s) 881, 945, 1979
AsuHPI GGTGA 1 cut(s) 1543
AvaII GGWCC 3 cut(s) 881, 945, 1979
BaeGI GKGCMC 1 cut(s) 1026
BanI GGYRCC 2 cut(s) 737, 1835
BauI CACGAG 2 cut(s) 134, 580
BbvI GCAGC 7 cut(s) 709, 945, 1529, 1801, 1804, 1807, 2043
BccI CCATC 7 cut(s) 139, 418, 689, 935, 1285, 1787, 2033
BciT130I CCWGG 2 cut(s) 686, 1784
BciVI GTATCC 3 cut(s) 992, 1473, 2090
BclI TGATCA 1 cut(s) 639
BcoDI GTCTC 2 cut(s) 1011, 2109
BcuI ACTAGT 2 cut(s) 932, 2030
BfaI CTAG 4 cut(s) 566, 717, 933, 2031
BfmI CTRYAG 2 cut(s) 915, 2013
BfoI RGCGCY 2 cut(s) 441, 1593
BfrI CTTAAG 2 cut(s) 898, 1996
BfuI GTATCC 3 cut(s) 992, 1473, 2090
BisI GCNGC 7 cut(s) 723, 959, 1518, 1815, 1818, 1821, 2057
BlsI GCNGC 7 cut(s) 724, 960, 1519, 1816, 1819, 1822, 2058
BmcAI AGTACT 1 cut(s) 1128
Bme1390I CCNGG 2 cut(s) 686, 1784
Bme18I GGWCC 3 cut(s) 881, 945, 1979
BmgBI CACGTC 3 cut(s) 1021, 1307, 2119
BmgT120I GGNCC 3 cut(s) 881, 945, 1979
BmiI GGNNCC 4 cut(s) 656, 739, 946, 1837
BmrFI CCNGG 2 cut(s) 686, 1784
BmsI GCATC 8 cut(s) 163, 180, 383, 740, 775, 1309, 1838, 1873
BoxI GACNNNNGTC 2 cut(s) 449, 1601
BpmI CTGGAG 1 cut(s) 1506
BpuEI CTTGAG 2 cut(s) 947, 2045
Bsa29I ATCGAT 1 cut(s) 1736
BsaJI CCNNGG 9 cut(s) 22, 447, 460, 1029, 1171, 1599, 1612, 2127, 2151
Bsc4I CCNNNNNNNGG 9 cut(s) 40, 107, 685, 734, 954, 1035, 1662, 1783, 1832
Bse118I RCCGGY 4 cut(s) 752, 814, 1850, 1912
Bse1I ACTGG 3 cut(s) 883, 1359, 1981
Bse3DI GCAATG 4 cut(s) 328, 1047, 1625, 2145
BseBI CCWGG 2 cut(s) 686, 1784
BseCI ATCGAT 1 cut(s) 1736
BseDI CCNNGG 9 cut(s) 22, 447, 460, 1029, 1171, 1599, 1612, 2127, 2151
BseGI GGATG 5 cut(s) 150, 605, 1296, 1324, 1631
BseLI CCNNNNNNNGG 9 cut(s) 40, 107, 685, 734, 954, 1035, 1662, 1783, 1832
BseMI GCAATG 4 cut(s) 328, 1047, 1625, 2145
BseMII CTCAG 2 cut(s) 283, 1242
BseNI ACTGG 3 cut(s) 883, 1359, 1981
BseRI GAGGAG 3 cut(s) 67, 152, 1210
BseSI GKGCMC 1 cut(s) 1026
BseXI GCAGC 7 cut(s) 709, 945, 1529, 1801, 1804, 1807, 2043
Bsh1236I CGCG 1 cut(s) 449
BshFI GGCC 4 cut(s) 27, 44, 68, 1176
BshNI GGYRCC 2 cut(s) 737, 1835
BshVI ATCGAT 1 cut(s) 1736
BsiSI CCGG 4 cut(s) 753, 815, 1851, 1913
BslFI GGGAC 2 cut(s) 958, 2056
BslI CCNNNNNNNGG 9 cut(s) 40, 107, 685, 734, 954, 1035, 1662, 1783, 1832
BsmAI GTCTC 2 cut(s) 1011, 2109
BsmBI CGTCTC 2 cut(s) 1011, 2109
BsmFI GGGAC 2 cut(s) 958, 2056
BsnI GGCC 4 cut(s) 27, 44, 68, 1176
Bsp1286I GDGCHC 1 cut(s) 1026
Bsp1407I TGTACA 3 cut(s) 226, 873, 1971
Bsp143I GATC 4 cut(s) 639, 1494, 1711, 1737
Bsp19I CCATGG 1 cut(s) 2151
BspACI CCGC 9 cut(s) 37, 287, 447, 449, 609, 646, 1026, 1986, 2172
BspANI GGCC 4 cut(s) 27, 44, 68, 1176
BspCNI CTCAG 2 cut(s) 282, 1243
BspDI ATCGAT 1 cut(s) 1736
BspFNI CGCG 1 cut(s) 449
BspLI GGNNCC 4 cut(s) 656, 739, 946, 1837
BspMAI CTGCAG 2 cut(s) 919, 2017
BspQI GCTCTTC 2 cut(s) 12, 1385
BspT107I GGYRCC 2 cut(s) 737, 1835
BspTI CTTAAG 2 cut(s) 898, 1996
BsrDI GCAATG 4 cut(s) 328, 1047, 1625, 2145
BsrFI RCCGGY 4 cut(s) 752, 814, 1850, 1912
BsrGI TGTACA 3 cut(s) 226, 873, 1971
BsrI ACTGG 3 cut(s) 883, 1359, 1981
BssAI RCCGGY 4 cut(s) 752, 814, 1850, 1912
BssECI CCNNGG 9 cut(s) 22, 447, 460, 1029, 1171, 1599, 1612, 2127, 2151
BssMI GATC 4 cut(s) 639, 1494, 1711, 1737
BssNAI GTATAC 1 cut(s) 1105
BssSI CACGAG 2 cut(s) 134, 580
BssT1I CCWWGG 7 cut(s) 22, 460, 1029, 1171, 1612, 2127, 2151
Bst1107I GTATAC 1 cut(s) 1105
Bst2BI CACGAG 2 cut(s) 134, 580
Bst2UI CCWGG 2 cut(s) 686, 1784
Bst4CI ACNGT 6 cut(s) 124, 499, 929, 1603, 1648, 2027
Bst6I CTCTTC 3 cut(s) 12, 1385, 1513
BstAFI CTTAAG 2 cut(s) 898, 1996
BstAPI GCANNNNNTGC 2 cut(s) 737, 1835
BstAUI TGTACA 3 cut(s) 226, 873, 1971
BstC8I GCNNGC 4 cut(s) 771, 1026, 1869, 2124
BstDEI CTNAG 4 cut(s) 269, 1251, 1569, 2046
BstDSI CCRYGG 3 cut(s) 447, 1599, 2151
BstF5I GGATG 5 cut(s) 150, 605, 1296, 1324, 1631
BstFNI CGCG 1 cut(s) 449
BstH2I RGCGCY 2 cut(s) 441, 1593
BstHHI GCGC 4 cut(s) 195, 321, 440, 1592
BstKTI GATC 4 cut(s) 642, 1497, 1714, 1740
BstMAI GTCTC 2 cut(s) 1011, 2109
BstMBI GATC 4 cut(s) 639, 1494, 1711, 1737
BstNI CCWGG 2 cut(s) 686, 1784
BstPAI GACNNNNGTC 2 cut(s) 449, 1601
BstSCI CCNGG 2 cut(s) 684, 1782
BstSFI CTRYAG 2 cut(s) 915, 2013
BstSLI GKGCMC 1 cut(s) 1026
BstUI CGCG 1 cut(s) 449
BstV1I GCAGC 7 cut(s) 709, 945, 1529, 1801, 1804, 1807, 2043
BstXI CCANNNNNNTGG 1 cut(s) 1150
BstZ17I GTATAC 1 cut(s) 1105
Bsu15I ATCGAT 1 cut(s) 1736
BsuI GTATCC 3 cut(s) 992, 1473, 2090
BsuRI GGCC 4 cut(s) 27, 44, 68, 1176
BsuTUI ATCGAT 1 cut(s) 1736
BtgI CCRYGG 3 cut(s) 447, 1599, 2151
BtrI CACGTC 3 cut(s) 1021, 1307, 2119
BtsCI GGATG 5 cut(s) 150, 605, 1296, 1324, 1631
BtsIMutI CAGTG 4 cut(s) 277, 876, 1366, 1974
Cac8I GCNNGC 4 cut(s) 771, 1026, 1869, 2124
CfoI GCGC 4 cut(s) 195, 321, 440, 1592
Cfr10I RCCGGY 4 cut(s) 752, 814, 1850, 1912
Cfr13I GGNCC 3 cut(s) 881, 945, 1979
Cfr42I CCGCGG 1 cut(s) 450
ClaI ATCGAT 1 cut(s) 1736
CseI GACGC 2 cut(s) 168, 848
DdeI CTNAG 4 cut(s) 269, 1251, 1569, 2046
DpnI GATC 4 cut(s) 641, 1496, 1713, 1739
DpnII GATC 4 cut(s) 639, 1494, 1711, 1737
DrdI GACNNNNNNGTC 1 cut(s) 1354
DseDI GACNNNNNNGTC 1 cut(s) 1354
Eam1104I CTCTTC 3 cut(s) 12, 1385, 1513
EarI CTCTTC 3 cut(s) 12, 1385, 1513
Eco130I CCWWGG 7 cut(s) 22, 460, 1029, 1171, 1612, 2127, 2151
Eco47I GGWCC 3 cut(s) 881, 945, 1979
Eco57I CTGAAG 2 cut(s) 687, 1785
EcoRI GAATTC 4 cut(s) 848, 967, 1946, 2065
EcoRII CCWGG 2 cut(s) 684, 1782
EcoT14I CCWWGG 7 cut(s) 22, 460, 1029, 1171, 1612, 2127, 2151
EcoT22I ATGCAT 2 cut(s) 790, 1888
ErhI CCWWGG 7 cut(s) 22, 460, 1029, 1171, 1612, 2127, 2151
Esp3I CGTCTC 2 cut(s) 1011, 2109
FaqI GGGAC 2 cut(s) 958, 2056
FauI CCCGC 1 cut(s) 1033
FbaI TGATCA 1 cut(s) 639
FblI GTMKAC 1 cut(s) 1104
Fnu4HI GCNGC 7 cut(s) 723, 959, 1518, 1815, 1818, 1821, 2057
FokI GGATG 5 cut(s) 157, 612, 1303, 1331, 1638
Fsp4HI GCNGC 7 cut(s) 723, 959, 1518, 1815, 1818, 1821, 2057
FspBI CTAG 4 cut(s) 566, 717, 933, 2031
FspI TGCGCA 1 cut(s) 320
GlaI GCGC 4 cut(s) 194, 320, 439, 1591
GluI GCNGC 7 cut(s) 723, 959, 1518, 1815, 1818, 1821, 2057
GsuI CTGGAG 1 cut(s) 1506
HaeII RGCGCY 2 cut(s) 441, 1593
HaeIII GGCC 4 cut(s) 27, 44, 68, 1176
HapII CCGG 4 cut(s) 753, 815, 1851, 1913
HgaI GACGC 2 cut(s) 168, 848
HhaI GCGC 4 cut(s) 195, 321, 440, 1592
Hin6I GCGC 4 cut(s) 193, 319, 438, 1590
HinP1I GCGC 4 cut(s) 193, 319, 438, 1590
HincII GTYRAC 2 cut(s) 1244, 1432
HindII GTYRAC 2 cut(s) 1244, 1432
HindIII AAGCTT 2 cut(s) 231, 1227
HinfI GANTC 2 cut(s) 362, 1462
HpaII CCGG 4 cut(s) 753, 815, 1851, 1913
HphI GGTGA 1 cut(s) 1543
Hpy166II GTNNAC 9 cut(s) 264, 874, 876, 1105, 1244, 1312, 1432, 1972, 1974
Hpy188I TCNGA 4 cut(s) 361, 556, 1252, 1705
Hpy188III TCNNGA 5 cut(s) 208, 454, 964, 1523, 2062
Hpy8I GTNNAC 9 cut(s) 264, 874, 876, 1105, 1244, 1312, 1432, 1972, 1974
Hpy99I CGWCG 1 cut(s) 1462
HpyAV CCTTC 9 cut(s) 79, 178, 506, 790, 1028, 1370, 1655, 1888, 2126
HpyCH4III ACNGT 6 cut(s) 124, 499, 929, 1603, 1648, 2027
HpyCH4IV ACGT 4 cut(s) 343, 1020, 1306, 2118
HpyF3I CTNAG 4 cut(s) 269, 1251, 1569, 2046
HpySE526I ACGT 4 cut(s) 343, 1020, 1306, 2118
HspAI GCGC 4 cut(s) 193, 319, 438, 1590
Ksp22I TGATCA 1 cut(s) 639
KspI CCGCGG 1 cut(s) 450
Kzo9I GATC 4 cut(s) 639, 1494, 1711, 1737
LguI GCTCTTC 2 cut(s) 12, 1385
LmnI GCTCC 6 cut(s) 80, 654, 730, 1223, 1525, 1828
Lsp1109I GCAGC 7 cut(s) 709, 945, 1529, 1801, 1804, 1807, 2043
LweI GCATC 8 cut(s) 163, 180, 383, 740, 775, 1309, 1838, 1873
MaeI CTAG 4 cut(s) 566, 717, 933, 2031
MaeII ACGT 4 cut(s) 343, 1020, 1306, 2118
MaeIII GTNAC 9 cut(s) 442, 575, 587, 661, 910, 1340, 1549, 1594, 2008
MalI GATC 4 cut(s) 641, 1496, 1713, 1739
MboI GATC 4 cut(s) 639, 1494, 1711, 1737
MboII GAAGA 7 cut(s) 405, 680, 794, 1402, 1500, 1778, 1892
MfeI CAATTG 1 cut(s) 1584
MhlI GDGCHC 1 cut(s) 1026
MlyI GAGTC 2 cut(s) 356, 1471
MmeI TCCRAC 4 cut(s) 384, 1094, 1251, 1527
Mph1103I ATGCAT 2 cut(s) 790, 1888
MseI TTAA 7 cut(s) 237, 519, 899, 1383, 1668, 1935, 1997
MslI CAYNNNNRTG 4 cut(s) 1052, 1305, 1556, 2150
MspA1I CMGCKG 1 cut(s) 449
MspCI CTTAAG 2 cut(s) 898, 1996
MspI CCGG 4 cut(s) 753, 815, 1851, 1913
MspR9I CCNGG 2 cut(s) 686, 1784
MunI CAATTG 1 cut(s) 1584
MvaI CCWGG 2 cut(s) 686, 1784
MvnI CGCG 1 cut(s) 449
NcoI CCATGG 1 cut(s) 2151
NdeII GATC 4 cut(s) 639, 1494, 1711, 1737
NlaIV GGNNCC 4 cut(s) 656, 739, 946, 1837
NmuCI GTSAC 6 cut(s) 442, 575, 910, 1549, 1594, 2008
NsbI TGCGCA 1 cut(s) 320
NsiI ATGCAT 2 cut(s) 790, 1888
OliI CACNNNNGTG 1 cut(s) 1556
PciSI GCTCTTC 2 cut(s) 12, 1385
PcsI WCGNNNNNNNCGW 1 cut(s) 1303
PflMI CCANNNNNTGG 2 cut(s) 107, 954
PkrI GCNGC 7 cut(s) 724, 960, 1519, 1816, 1819, 1822, 2058
PleI GAGTC 2 cut(s) 356, 1470
PpsI GAGTC 2 cut(s) 356, 1470
PshAI GACNNNNGTC 2 cut(s) 449, 1601
PsiI TTATAA 1 cut(s) 168
Psp1406I AACGTT 1 cut(s) 343
Psp6I CCWGG 2 cut(s) 684, 1782
PspGI CCWGG 2 cut(s) 684, 1782
PspN4I GGNNCC 4 cut(s) 656, 739, 946, 1837
PspPI GGNCC 3 cut(s) 881, 945, 1979
PstI CTGCAG 2 cut(s) 919, 2017
RseI CAYNNNNRTG 4 cut(s) 1052, 1305, 1556, 2150
SacII CCGCGG 1 cut(s) 450
SapI GCTCTTC 2 cut(s) 12, 1385
SaqAI TTAA 7 cut(s) 237, 519, 899, 1383, 1668, 1935, 1997
SatI GCNGC 7 cut(s) 723, 959, 1518, 1815, 1818, 1821, 2057
Sau3AI GATC 4 cut(s) 639, 1494, 1711, 1737
Sau96I GGNCC 3 cut(s) 881, 945, 1979
ScaI AGTACT 1 cut(s) 1128
SchI GAGTC 2 cut(s) 356, 1471
ScrFI CCNGG 2 cut(s) 686, 1784
SduI GDGCHC 1 cut(s) 1026
SfaNI GCATC 8 cut(s) 163, 180, 383, 740, 775, 1309, 1838, 1873
SfcI CTRYAG 2 cut(s) 915, 2013
Sfr303I CCGCGG 1 cut(s) 450
SgrBI CCGCGG 1 cut(s) 450
SinI GGWCC 3 cut(s) 881, 945, 1979
SmiMI CAYNNNNRTG 4 cut(s) 1052, 1305, 1556, 2150
SmlI CTYRAG 4 cut(s) 898, 962, 1996, 2060
SmoI CTYRAG 4 cut(s) 898, 962, 1996, 2060
SpeI ACTAGT 2 cut(s) 932, 2030
SsiI CCGC 9 cut(s) 37, 287, 447, 449, 609, 646, 1026, 1986, 2172
SspMI CTAG 4 cut(s) 566, 717, 933, 2031
StyD4I CCNGG 2 cut(s) 684, 1782
StyI CCWWGG 7 cut(s) 22, 460, 1029, 1171, 1612, 2127, 2151
TaaI ACNGT 6 cut(s) 124, 499, 929, 1603, 1648, 2027
TaiI ACGT 4 cut(s) 346, 1023, 1309, 2121
TaqI TCGA 6 cut(s) 80, 385, 1297, 1537, 1736, 1961
TatI WGTACW 6 cut(s) 226, 261, 873, 1126, 1404, 1971
Tru1I TTAA 7 cut(s) 237, 519, 899, 1383, 1668, 1935, 1997
Tru9I TTAA 7 cut(s) 237, 519, 899, 1383, 1668, 1935, 1997
TscAI CASTG 4 cut(s) 277, 883, 1366, 1981
TseFI GTSAC 6 cut(s) 442, 575, 910, 1549, 1594, 2008
TseI GCWGC 7 cut(s) 722, 958, 1517, 1814, 1817, 1820, 2056
Tsp45I GTSAC 6 cut(s) 442, 575, 910, 1549, 1594, 2008
TspDTI ATGAA 4 cut(s) 343, 921, 1557, 2019
TspGWI ACGGA 1 cut(s) 612
TspRI CASTG 4 cut(s) 277, 883, 1366, 1981
Van91I CCANNNNNTGG 2 cut(s) 107, 954
Vha464I CTTAAG 2 cut(s) 898, 1996
VpaK11BI GGWCC 3 cut(s) 881, 945, 1979
XapI RAATTY 9 cut(s) 221, 306, 702, 848, 967, 1448, 1800, 1946, 2065
XcmI CCANNNNNNNNNTGG 3 cut(s) 692, 1619, 1790
XmiI GTMKAC 1 cut(s) 1104
XspI CTAG 4 cut(s) 566, 717, 933, 2031
ZrmI AGTACT 1 cut(s) 1128
Zsp2I ATGCAT 2 cut(s) 790, 1888
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.