Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr4

Orthogroup ID

OG0011572

Physical Location & Seq

Genomic Coordinates Reverse (-)

20758887 .. 20762492

Gene Structure 3606 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1593 bp

ATGACTCGCGGCACCCTCCCGTTGGCAGCTTCGGATCTTCATATCATGGATGGAACTCCAAGAAATTCCACACTGATATGTGCGCCGGTAGTGGCGGATTCAGTTGATCAGATGCTGGTCCAAATGGGGAAGGCAAAGGAAGTTGGTGCTGACCTCGTTGAACTTCGGGTTGATTTTTTGAAGAACTTCAGTCCAAGACACGACCTCAGTGTATTGATCAAACAAAGTCCTTTGCCTACCCTTGTCACTTACAGGCCAGCATGGGAAGGAGGTCAATACAAGGGTGACGACAAGCAGCGACAAGATGCACTGCATGTAGCCATGGATTTGGGAGCGGATTTTATTGATGTCGAACTTAAGGTAGCCGACGAGTTCTACAATTCCATTCAAGGGAGGAAGCCAGAAAAGGTCAAAATCATTGTTTCTTCACACAACTATGAGAAAACTCCATCCGCTGAGGAAATTGGTGATCTTGTTGCAAGGATACAAGCTACGGGAGCTGACATAGTGAAGATCGCAACAACTGGCTTAGACATCACCGACTCTGCACGTGTATTTCAAGTATTAGCGCATTCTCAAGTCCCAATGATAGGGCTTGTTATGGGAGAGAAGGGTTTGATCTCACGCGTACTTAGTGCAAAATATGGTGCATTCCTCACTTTTGGTACAATAGAGGCGGGAGTAGTATCGGCTCCTGGCCAGCCCACCGTAAGAGATCTATTGGACTTATACAACTTCAGACTTATAGGGGCCGATACCAAAGTACTTGGTGTGATAGGAAATCCTATCAGTCACAGCAAAATCCCTCATCTTTACAATGCAGCATTCAGATCTATCAATTTCAATGGAATTTATTTGCCTTTGTTGGTTGATAACGTTGCGAATTTTATTGACACTTATAACTCCCCCGACTTTGTTGGATACAGAATTCCTCACAACGACGCTGGGATTAAATGCTGTGATGAAATCGATCCAAATGCCAAGGAAATAGGAGCTATTAGTTGCATGATCAGGAACCCGGCTGATGGGAAATTAAAGGGCTACAATGTGGATTATCTTGGAGCAATTGCAGCCATTGAGGAAGGACTTCAAGCATTAAATGGTTCAAGTAATGGATCTGGTTCCCAGTTAGCTGGTAAACTATTTGTCGTCATGGGAGCTGGTGGTGCTGGAAAGGCACTTGCACATGGTGGAAAACAGAAAGGAGCAAGAGTGGTAGTTGCCAATCGCACATATGACAAAGCCAAGGCACTTGCAAACAAAGTTGGAGGAGAAGCTATCACTCTTGCTGAATTGGAAAATTTCCATCCAGAGGAGGGAATGGTTCTTGCAAACACCACATCGGTTGGAATGAAGCCGAGAATTGATCAAACACCCTTATCCAAGAACGCTTTGAAGAACTATTCTTTGGTGTTTGATGCCATTCTTACACCTAAATGGACAAGACTCTTACAAGAAGCACAAGAGTCTGGTGCAGCCGTTGTTTCCGGGACGGAAATGTTCCTCAATCAAGCATTTGTACAGGTTGAAAAGTTTTCTGGTTCACCTGCACCAAAGCAACTGATCAGGGATGTGTTGGCAAGAAATGCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004764 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009423 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0016053 GO:0016491 GO:0016614 GO:0016616 GO:0019632 GO:0019752 GO:0022414 GO:0032501 GO:0032502 GO:0032787 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043648 GO:0043650 GO:0044237 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046417 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0055114 GO:0061458 GO:0071704 GO:1901576 GO:1901615

KEGG Pathways

Metabolic & Signaling

ko00400 ko01100 ko01110 ko01130 ko01230 map00400 map01100 map01110 map01130 map01230

Pfam Domains

Protein Families

DHquinase_I Shikimate_DH Shikimate_dh_N

Protein Analysis

531

Amino Acids

56.87

Weight (kDa)

6.39

Isoelectric Point (pI)

22.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DHquinase_I	PF01487	27 - 244	7e-67	Type I 3-dehydroquinase
Shikimate_dh_N	PF08501	258 - 334	1.1e-13	Shikimate dehydrogenase substrate binding domain
Shikimate_DH	PF01488	376 - 448	1.3e-08	Shikimate / quinate 5-dehydrogenase
SDH_C	PF18317	497 - 526	2.5e-06	Shikimate 5'-dehydrogenase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0011572)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g04670 FvH4_6g04670 FvH4_6g04670
malus_domestica	MD04G1213800.v1.1 MD12G1228800.v1.1
prunus_persica	Prupe.6G334000_v2.0.a1 Prupe.6G334000_v2.0.a1
pyrus_communis	pycom04g18930 pycom12g21340
rosa_chinensis	RchiOBHm_Chr3g0453141
rosa_laevigata	RLG00000025534
rosa_multiflora	Rmu_sc0007132.1_g000006 Rmu_sc0013481.1_g000002
rosa_roxburghii	Rroxscaffold_6G00430200
rosa_rugosa	Rorug02G0646800
rosa_samantha	Rh3AG049400 Rh3BG050600 Rh3CG050800 Rh3DG051400
rosa_wichuraiana	Rw3G003790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	900
AarI	CACCTGC	1 cut(s)	1555
Acc36I	ACCTGC	1 cut(s)	1555
AccB1I	GGYRCC	1 cut(s)	11
AccBSI	CCGCTC	1 cut(s)	335
AccII	CGCG	2 cut(s)	9, 627
AciI	CCGC	5 cut(s)	9, 95, 335, 453, 677
AclI	AACGTT	1 cut(s)	876
AclWI	GGATC	3 cut(s)	42, 965, 1123
AcoI	YGGCCR	1 cut(s)	697
AcsI	RAATTY	5 cut(s)	64, 849, 883, 927, 1300
AcuI	CTGAAG	2 cut(s)	172, 721
AcvI	CACGTG	1 cut(s)	551
AdeI	CACNNNGTG	1 cut(s)	1190
AfaI	GTAC	4 cut(s)	630, 667, 765, 1521
AfiI	CCNNNNNNNGG	6 cut(s)	22, 390, 590, 1025, 1312, 1316
AflII	CTTAAG	1 cut(s)	356
AflIII	ACRYGT	2 cut(s)	550, 625
AgsI	TTSAA	9 cut(s)	161, 181, 389, 560, 844, 1091, 1107, 1396, 1529
AjnI	CCWGG	1 cut(s)	694
AjuI	GAANNNNNNNTTGG	2 cut(s)	1391, 1423
AluBI	AGCT	7 cut(s)	29, 491, 500, 995, 1133, 1160, 1277
AluI	AGCT	7 cut(s)	29, 491, 500, 995, 1133, 1160, 1277
AlwI	GGATC	3 cut(s)	42, 965, 1123
AlwNI	CAGNNNCTG	1 cut(s)	115
AoxI	GGCC	3 cut(s)	254, 697, 750
ApeKI	GCWGC	5 cut(s)	26, 295, 821, 1070, 1475
ApoI	RAATTY	5 cut(s)	64, 849, 883, 927, 1300
Asp700I	GAANNNNTTC	2 cut(s)	185, 1086
AspLEI	GCGC	2 cut(s)	85, 571
AspS9I	GGNCC	2 cut(s)	118, 750
AsuC2I	CCSGG	2 cut(s)	1019, 1489
AsuHPI	GGTGA	4 cut(s)	296, 479, 529, 1536
AvaII	GGWCC	1 cut(s)	118
BaeI	ACNNNNGTAYC	2 cut(s)	747, 780
BalI	TGGCCA	1 cut(s)	699
BanI	GGYRCC	1 cut(s)	11
BbrPI	CACGTG	1 cut(s)	551
BbvCI	CCTCAGC	1 cut(s)	456
BbvI	GCAGC	5 cut(s)	38, 307, 833, 1082, 1487
BccI	CCATC	4 cut(s)	44, 457, 1019, 1314
BceAI	ACGGC	1 cut(s)	1463
BcgI	CGANNNNNNTGC	2 cut(s)	959, 993
BciT130I	CCWGG	1 cut(s)	696
BciVI	GTATCC	2 cut(s)	477, 914
BclI	TGATCA	5 cut(s)	106, 216, 1008, 1366, 1563
BcnI	CCSGG	2 cut(s)	1019, 1489
BfrI	CTTAAG	1 cut(s)	356
BfuAI	ACCTGC	1 cut(s)	1555
BfuI	GTATCC	2 cut(s)	477, 914
BglII	AGATCT	2 cut(s)	715, 830
BisI	GCNGC	6 cut(s)	10, 27, 296, 822, 1071, 1476
BlsI	GCNGC	6 cut(s)	11, 28, 297, 823, 1072, 1477
BmcAI	AGTACT	1 cut(s)	765
Bme1390I	CCNGG	3 cut(s)	696, 1019, 1489
Bme18I	GGWCC	1 cut(s)	118
BmgT120I	GGNCC	2 cut(s)	118, 750
BmiI	GGNNCC	5 cut(s)	13, 693, 751, 1016, 1123
BmrFI	CCNGG	3 cut(s)	696, 1019, 1489
BmrI	ACTGGG	1 cut(s)	1120
BmsI	GCATC	3 cut(s)	102, 295, 1408
BmuI	ACTGGG	1 cut(s)	1120
Bpu10I	CCTNAGC	1 cut(s)	456
BpuEI	CTTGAG	1 cut(s)	561
BpuMI	CCSGG	2 cut(s)	1019, 1489
Bsa29I	ATCGAT	1 cut(s)	969
BsaAI	YACGTR	1 cut(s)	551
BsaJI	CCNNGG	3 cut(s)	321, 981, 1245
BsaXI	ACNNNNNCTCC	2 cut(s)	1197, 1227
Bsc4I	CCNNNNNNNGG	6 cut(s)	22, 390, 590, 1025, 1312, 1316
Bse118I	RCCGGY	1 cut(s)	85
Bse1I	ACTGG	2 cut(s)	529, 1126
BseBI	CCWGG	1 cut(s)	696
BseCI	ATCGAT	1 cut(s)	969
BseDI	CCNNGG	3 cut(s)	321, 981, 1245
BseGI	GGATG	4 cut(s)	55, 449, 1306, 1576
BseLI	CCNNNNNNNGG	6 cut(s)	22, 390, 590, 1025, 1312, 1316
BseMII	CTCAG	2 cut(s)	220, 447
BseNI	ACTGG	2 cut(s)	529, 1126
BseRI	GAGGAG	2 cut(s)	1284, 1328
BseXI	GCAGC	5 cut(s)	38, 307, 833, 1082, 1487
BseYI	CCCAGC	1 cut(s)	944
BsgI	GTGCAG	3 cut(s)	531, 1494, 1533
Bsh1236I	CGCG	2 cut(s)	9, 627
BshFI	GGCC	3 cut(s)	256, 699, 752
BshNI	GGYRCC	1 cut(s)	11
BshVI	ATCGAT	1 cut(s)	969
BsiSI	CCGG	3 cut(s)	86, 1019, 1488
BslFI	GGGAC	2 cut(s)	566, 1504
BslI	CCNNNNNNNGG	6 cut(s)	22, 390, 590, 1025, 1312, 1316
BsmFI	GGGAC	2 cut(s)	566, 1504
BsmI	GAATGC	3 cut(s)	571, 650, 824
BsnI	GGCC	3 cut(s)	256, 699, 752
Bsp1407I	TGTACA	1 cut(s)	1519
Bsp19I	CCATGG	1 cut(s)	321
BspACI	CCGC	5 cut(s)	9, 95, 335, 453, 677
BspANI	GGCC	3 cut(s)	256, 699, 752
BspCNI	CTCAG	2 cut(s)	219, 448
BspDI	ATCGAT	1 cut(s)	969
BspFNI	CGCG	2 cut(s)	9, 627
BspLI	GGNNCC	5 cut(s)	13, 693, 751, 1016, 1123
BspMI	ACCTGC	1 cut(s)	1555
BspPI	GGATC	3 cut(s)	42, 965, 1123
BspT107I	GGYRCC	1 cut(s)	11
BspTI	CTTAAG	1 cut(s)	356
BsrBI	CCGCTC	1 cut(s)	335
BsrFI	RCCGGY	1 cut(s)	85
BsrGI	TGTACA	1 cut(s)	1519
BsrI	ACTGG	2 cut(s)	529, 1126
BssAI	RCCGGY	1 cut(s)	85
BssECI	CCNNGG	3 cut(s)	321, 981, 1245
BssT1I	CCWWGG	3 cut(s)	321, 981, 1245
Bst2UI	CCWGG	1 cut(s)	696
Bst4CI	ACNGT	1 cut(s)	709
BstAFI	CTTAAG	1 cut(s)	356
BstAPI	GCANNNNNTGC	1 cut(s)	1586
BstAUI	TGTACA	1 cut(s)	1519
BstBAI	YACGTR	1 cut(s)	551
BstC8I	GCNNGC	2 cut(s)	258, 701
BstDEI	CTNAG	4 cut(s)	206, 456, 529, 632
BstDSI	CCRYGG	1 cut(s)	321
BstF5I	GGATG	4 cut(s)	55, 449, 1306, 1576
BstFNI	CGCG	2 cut(s)	9, 627
BstHHI	GCGC	2 cut(s)	85, 571
BstMWI	GCNNNNNNNGC	5 cut(s)	497, 1070, 1166, 1175, 1586
BstNI	CCWGG	1 cut(s)	696
BstNSI	RCATGY	1 cut(s)	317
BstSCI	CCNGG	3 cut(s)	694, 1017, 1487
BstUI	CGCG	2 cut(s)	9, 627
BstV1I	GCAGC	5 cut(s)	38, 307, 833, 1082, 1487
BstX2I	RGATCY	4 cut(s)	34, 715, 830, 1115
BstXI	CCANNNNNNTGG	2 cut(s)	328, 1133
BstYI	RGATCY	4 cut(s)	34, 715, 830, 1115
Bsu15I	ATCGAT	1 cut(s)	969
BsuI	GTATCC	2 cut(s)	477, 914
BsuRI	GGCC	3 cut(s)	256, 699, 752
BsuTUI	ATCGAT	1 cut(s)	969
BtgI	CCRYGG	1 cut(s)	321
BtsCI	GGATG	4 cut(s)	55, 449, 1306, 1576
BtsI	GCAGTG	1 cut(s)	308
BtsIMutI	CAGTG	3 cut(s)	71, 214, 308
BveI	ACCTGC	1 cut(s)	1555
Cac8I	GCNNGC	2 cut(s)	258, 701
CaiI	CAGNNNCTG	1 cut(s)	115
CfoI	GCGC	2 cut(s)	85, 571
Cfr10I	RCCGGY	1 cut(s)	85
Cfr13I	GGNCC	2 cut(s)	118, 750
ClaI	ATCGAT	1 cut(s)	969
CseI	GACGC	1 cut(s)	950
Csp6I	GTAC	4 cut(s)	629, 666, 764, 1520
CspCI	CAANNNNNGTGG	2 cut(s)	1327, 1362
CviAII	CATG	8 cut(s)	46, 261, 314, 322, 1006, 1153, 1187, 1590
CviQI	GTAC	4 cut(s)	629, 666, 764, 1520
DdeI	CTNAG	4 cut(s)	206, 456, 529, 632
DraIII	CACNNNGTG	1 cut(s)	1190
EaeI	YGGCCR	1 cut(s)	697
EciI	GGCGGA	1 cut(s)	110
Eco130I	CCWWGG	3 cut(s)	321, 981, 1245
Eco47I	GGWCC	1 cut(s)	118
Eco57I	CTGAAG	2 cut(s)	172, 721
Eco72I	CACGTG	1 cut(s)	551
EcoRI	GAATTC	1 cut(s)	927
EcoRII	CCWGG	1 cut(s)	694
EcoT14I	CCWWGG	3 cut(s)	321, 981, 1245
EcoT22I	ATGCAT	1 cut(s)	1591
ErhI	CCWWGG	3 cut(s)	321, 981, 1245
FaeI	CATG	8 cut(s)	49, 264, 317, 325, 1009, 1156, 1190, 1593
FaqI	GGGAC	2 cut(s)	566, 1504
FatI	CATG	8 cut(s)	45, 260, 313, 321, 1005, 1152, 1186, 1589
FauI	CCCGC	1 cut(s)	670
FauNDI	CATATG	1 cut(s)	1234
FbaI	TGATCA	5 cut(s)	106, 216, 1008, 1366, 1563
Fnu4HI	GCNGC	6 cut(s)	10, 27, 296, 822, 1071, 1476
FokI	GGATG	4 cut(s)	62, 436, 1293, 1583
Fsp4HI	GCNGC	6 cut(s)	10, 27, 296, 822, 1071, 1476
GlaI	GCGC	2 cut(s)	84, 570
GluI	GCNGC	6 cut(s)	10, 27, 296, 822, 1071, 1476
GsaI	CCCAGC	1 cut(s)	948
HaeIII	GGCC	3 cut(s)	256, 699, 752
HapII	CCGG	3 cut(s)	86, 1019, 1488
HgaI	GACGC	1 cut(s)	950
HhaI	GCGC	2 cut(s)	85, 571
Hin1II	CATG	8 cut(s)	49, 264, 317, 325, 1009, 1156, 1190, 1593
Hin6I	GCGC	2 cut(s)	83, 569
HinP1I	GCGC	2 cut(s)	83, 569
HinfI	GANTC	5 cut(s)	4, 98, 542, 1446, 1466
HpaII	CCGG	3 cut(s)	86, 1019, 1488
HphI	GGTGA	4 cut(s)	296, 479, 529, 1536
Hpy166II	GTNNAC	2 cut(s)	1139, 1544
Hpy188I	TCNGA	4 cut(s)	34, 111, 740, 830
Hpy188III	TCNNGA	2 cut(s)	1012, 1310
Hpy8I	GTNNAC	2 cut(s)	1139, 1544
Hpy99I	CGWCG	2 cut(s)	371, 944
HpyAV	CCTTC	4 cut(s)	124, 260, 604, 1076
HpyCH4III	ACNGT	1 cut(s)	709
HpyCH4IV	ACGT	2 cut(s)	550, 876
HpyF10VI	GCNNNNNNNGC	5 cut(s)	497, 1070, 1166, 1175, 1586
HpyF3I	CTNAG	4 cut(s)	206, 456, 529, 632
HpySE526I	ACGT	2 cut(s)	550, 876
Hsp92II	CATG	8 cut(s)	49, 264, 317, 325, 1009, 1156, 1190, 1593
HspAI	GCGC	2 cut(s)	83, 569
Ksp22I	TGATCA	5 cut(s)	106, 216, 1008, 1366, 1563
LmnI	GCTCC	7 cut(s)	332, 497, 697, 992, 1061, 1157, 1205
Lsp1109I	GCAGC	5 cut(s)	38, 307, 833, 1082, 1487
LweI	GCATC	3 cut(s)	102, 295, 1408
MaeII	ACGT	2 cut(s)	550, 876
MaeIII	GTNAC	3 cut(s)	244, 284, 791
MbiI	CCGCTC	1 cut(s)	335
MboII	GAAGA	5 cut(s)	29, 193, 417, 523, 1408
MfeI	CAATTG	1 cut(s)	1065
MflI	RGATCY	4 cut(s)	34, 715, 830, 1115
MlsI	TGGCCA	1 cut(s)	699
MluI	ACGCGT	1 cut(s)	625
MluNI	TGGCCA	1 cut(s)	699
MlyI	GAGTC	3 cut(s)	536, 1440, 1475
MmeI	TCCRAC	3 cut(s)	898, 1246, 1327
Mox20I	TGGCCA	1 cut(s)	699
Mph1103I	ATGCAT	1 cut(s)	1591
MroXI	GAANNNNTTC	2 cut(s)	185, 1086
MscI	TGGCCA	1 cut(s)	699
MseI	TTAA	4 cut(s)	357, 951, 1034, 1097
MslI	CAYNNNNRTG	3 cut(s)	76, 435, 1435
Msp20I	TGGCCA	1 cut(s)	699
MspA1I	CMGCKG	1 cut(s)	455
MspCI	CTTAAG	1 cut(s)	356
MspI	CCGG	3 cut(s)	86, 1019, 1488
MspR9I	CCNGG	3 cut(s)	696, 1019, 1489
MunI	CAATTG	1 cut(s)	1065
Mva1269I	GAATGC	3 cut(s)	571, 650, 824
MvaI	CCWGG	1 cut(s)	696
MvnI	CGCG	2 cut(s)	9, 627
MwoI	GCNNNNNNNGC	5 cut(s)	497, 1070, 1166, 1175, 1586
NciI	CCSGG	2 cut(s)	1019, 1489
NcoI	CCATGG	1 cut(s)	321
NdeI	CATATG	1 cut(s)	1234
NlaIII	CATG	8 cut(s)	49, 264, 317, 325, 1009, 1156, 1190, 1593
NlaIV	GGNNCC	5 cut(s)	13, 693, 751, 1016, 1123
NmeAIII	GCCGAG	1 cut(s)	1383
NmuCI	GTSAC	3 cut(s)	244, 284, 791
NsiI	ATGCAT	1 cut(s)	1591
NspI	RCATGY	1 cut(s)	317
PaqCI	CACCTGC	1 cut(s)	1555
PctI	GAATGC	3 cut(s)	571, 650, 824
PdmI	GAANNNNTTC	2 cut(s)	185, 1086
PfeI	GAWTC	1 cut(s)	98
PfoI	TCCNGGA	1 cut(s)	1487
PkrI	GCNGC	6 cut(s)	11, 28, 297, 823, 1072, 1477
PleI	GAGTC	3 cut(s)	536, 1440, 1474
PmaCI	CACGTG	1 cut(s)	551
PmlI	CACGTG	1 cut(s)	551
PpsI	GAGTC	3 cut(s)	536, 1440, 1474
Ppu21I	YACGTR	1 cut(s)	551
PsiI	TTATAA	1 cut(s)	900
Psp1406I	AACGTT	1 cut(s)	876
Psp6I	CCWGG	1 cut(s)	694
PspCI	CACGTG	1 cut(s)	551
PspFI	CCCAGC	1 cut(s)	944
PspGI	CCWGG	1 cut(s)	694
PspN4I	GGNNCC	5 cut(s)	13, 693, 751, 1016, 1123
PspPI	GGNCC	2 cut(s)	118, 750
PstNI	CAGNNNCTG	1 cut(s)	115
PsuI	RGATCY	4 cut(s)	34, 715, 830, 1115
RsaI	GTAC	4 cut(s)	630, 667, 765, 1521
RsaNI	GTAC	4 cut(s)	629, 666, 764, 1520
RseI	CAYNNNNRTG	3 cut(s)	76, 435, 1435
SaqAI	TTAA	4 cut(s)	357, 951, 1034, 1097
SatI	GCNGC	6 cut(s)	10, 27, 296, 822, 1071, 1476
Sau96I	GGNCC	2 cut(s)	118, 750
ScaI	AGTACT	1 cut(s)	765
SchI	GAGTC	3 cut(s)	536, 1440, 1475
ScrFI	CCNGG	3 cut(s)	696, 1019, 1489
SfaNI	GCATC	3 cut(s)	102, 295, 1408
SinI	GGWCC	1 cut(s)	118
SmiMI	CAYNNNNRTG	3 cut(s)	76, 435, 1435
SmlI	CTYRAG	2 cut(s)	356, 576
SmoI	CTYRAG	2 cut(s)	356, 576
SsiI	CCGC	5 cut(s)	9, 95, 335, 453, 677
StyD4I	CCNGG	3 cut(s)	694, 1017, 1487
StyI	CCWWGG	3 cut(s)	321, 981, 1245
TaaI	ACNGT	1 cut(s)	709
TaiI	ACGT	2 cut(s)	553, 879
TaqI	TCGA	2 cut(s)	351, 969
TatI	WGTACW	2 cut(s)	763, 1519
TauI	GCSGC	1 cut(s)	12
TfiI	GAWTC	1 cut(s)	98
Tru1I	TTAA	4 cut(s)	357, 951, 1034, 1097
Tru9I	TTAA	4 cut(s)	357, 951, 1034, 1097
TscAI	CASTG	3 cut(s)	78, 214, 315
TseFI	GTSAC	3 cut(s)	244, 284, 791
TseI	GCWGC	5 cut(s)	26, 295, 821, 1070, 1475
Tsp45I	GTSAC	3 cut(s)	244, 284, 791
TspDTI	ATGAA	3 cut(s)	29, 978, 1367
TspGWI	ACGGA	1 cut(s)	1508
TspRI	CASTG	3 cut(s)	78, 214, 315
Vha464I	CTTAAG	1 cut(s)	356
VpaK11BI	GGWCC	1 cut(s)	118
XapI	RAATTY	5 cut(s)	64, 849, 883, 927, 1300
XceI	RCATGY	1 cut(s)	317
XmnI	GAANNNNTTC	2 cut(s)	185, 1086
ZrmI	AGTACT	1 cut(s)	765
Zsp2I	ATGCAT	1 cut(s)	1591

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.