pycom05g00170
ERF Family

Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin Fzo YdjA family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0012009
Physical Location & Seq
Forward (+)
201434 .. 210721
9288 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2382 bp
ATGGAGGCGATCGAGGAATTGTTGCAGCTATCAGACTCGATGCGGCAAGGCACCGCTATCCTTGCCGACGAAGATGTGGACGAGAACACAACCTCCTCTCGCAGGGCTTCCAGTTTCCTTAACGTCGTCGCTCTCGGCAATGTTGGTGCGGGTAAATCAGCAGTTTTGAACAGTTTGATTGGACATCCTGTTTTGCCAACTGGTGAAAATGGTGCTACTCGAGCTCCCATTAGCATTGATTTGCAGAGGGATGGTTCCTTGAGCAGCAAATCTATTATATTGCAGATTGACAATAAATCTCAGCAAGTTTCTGCAAGTGCTTTACGGCATTCTCTGCAAGGTAGGCTGGGAAACGCCACAGGAAAAACCCGTGATGAAATTAATTTGAAGCTACGAACAAGTACAGCCCCACCCCTCAAATTGATTGATTTACCTGGGCTTGATCAAAGGATCATGGATGAGTCTATGATTAGTGAATATGCTGAGCACAATGATTCAATTTTGCTCGTTATAGTTCCTGCATCTCAAGCACCTGAAGTTGCTTCATCTCGAGCCCTCAGAGTCGTCAAGGAGTATGATGCAGATGGTACCAGAACTGTTGGTGTTATTAGCAAGATAGACCAAGCTGCTTCAGATCAGAAAGCACTTTCTGCAGTCCAGGCTCTTCTGTTAAATCAAGGACCATCAAGAGCATCTGATATCCCTTGGGTTGCTTTAATAGGTCAATCAGTTTCAATAGCTTCTGCACAGTCTGGAGGTTCTGAAAGTTCTCTAGAAACCGCCTGGAGGGCTGAAAGTGAAAGTCTGAAGTCTATTTTGACCGGAGCTCCTCAAAGTAAGCTAGGCAGAATAGCGTTGGTGGATGCTCTGGCACAGCAGATTCGTAGCCGTATGAAAGTTCGACTTCCAAACCTCCTTTCTGGGTTACAAGGGAAGTCTCAAATAGTTCAGGATGAGTTAGTAAAGCTTGGTGCATCTATGGTGCAGAGTGCTGAGGGTACCAGATCCCTAGCTTTGGAGCTTTGCCGTGAGTTTGAGGATAAGTTTCTCCAACACATTACAACTGGTGAGGGTTCTGGATGGAAAATTGTTGCAAGTTTTGAAGGAAATTTCCCAAATAGAATAAAACAACTCCCATTAGACCGACATTTTGATATCAATAATGTGAAGAGGATTGTGTTGGAAGCAGATGGTTATCAACCTTATCTCATATCCCCTGAAAAGGGGTTGAGGTCTTTAATAAAAGGGGTCTTAGAGCTTGCAAAGGAGCCATCACGTCTTTGTGTGGATGAGGTACATCGTGTCCTGGTGGATATAGTTTCTGCTGCTGCTAATGCTACGCCAGGTCTTGGAAGATACCCTCCTTTCAAGAGGGAGGTTGTGGCCATTGCAAGTGCTGCATTGGATGCGTTTAAGAATGAAGCAAAGAAAATGGTGGTTGCACTTGTAGATATGGAACGTGCTTTTGTGCCTCCCCAACACTTCATTCGCTTGGTGCAAAGGAGGATGGAGAGGCAGCGTAGAGAGGAAGAAGTGAAAAATCGATCTTCGAAGAAAGGACAAGAGGCAGAGCAATCAATAATGAATCGGGCAACTAGTCCTCAAACAGGGGGGGAACAAGCTGGTGGAAGCATGAAATCAATGAAGGATAAGGATAAATCTGGTAAGGACGGCAAAGAAGTACAGGAGGCAACTGGTTTGAAGACTGCAGGTCCTGAAGGAGAAATAACAGCAGGTTTCTTGTATAAAAAAAGTGCCAAAACTAATGGATGGAGCAAGCGATGGTTTGTTTTGAATGAAAAGACTGGAAAGCTTGGTTACACCAAAAAGCAAGAGGAGAGACACTTCCGTGGTGTCATCACTTTGGAGGAGTGTAATATTGAAGAGGCTTCAGATGAGGATGAACCACCTCCATCGAAGAGCTCAAAGGACAAAAAGTCAAATGGACCAGAAAAATCACCCAGTCTTGTGTTTAAGATAACTAGCAAAGTTCCATATAAAACTGTTCTGAAAGCGCACAGCGCCGTTGTGCTAAAGGCAGAGACTGTGGCAGACAAGCTAGAGTGGATAAATAAGATTGGAAAGGTTATTCAACCTTCTAAAGGACTAGTGAGGGGTGCTTCAACTGAAGGCGGTCCTACAATGCGACAAAGTCTTTCTGATGGTTCTCTTGATACAATGACTCGAAGACCTGCTGACCCTGAGGAAGAACTCCGGTGGATGTCTCAAGAAGTTCGTGGTTATGTTGAAGCTGTTTTGAATAGTTTGGCTGCTAATGTCCCTAAGGCAGTTGTTCTTTGCCAAGTTGAAAAGGCCAAGGAAGACATGTTAAATCAGCTATATAGCTCTGTCAGCCTTTGCTCAAAGCACAGCAAAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000266 GO:0000902 GO:0000904 GO:0001101 GO:0003674 GO:0003824 GO:0003924 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005829 GO:0005886 GO:0005911 GO:0006810 GO:0006892 GO:0006896 GO:0006897 GO:0006898 GO:0006996 GO:0007005 GO:0007034 GO:0007275 GO:0008017 GO:0008092 GO:0008150 GO:0009504 GO:0009506 GO:0009653 GO:0009719 GO:0009725 GO:0009737 GO:0009888 GO:0009987 GO:0010015 GO:0010033 GO:0010053 GO:0010054 GO:0012505 GO:0015631 GO:0016020 GO:0016043 GO:0016192 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017111 GO:0021700 GO:0022622 GO:0030054 GO:0030135 GO:0030136 GO:0030139 GO:0030154 GO:0030276 GO:0031090 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0032878 GO:0032989 GO:0033993 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045334 GO:0046907 GO:0048193 GO:0048285 GO:0048364 GO:0048468 GO:0048469 GO:0048731 GO:0048764 GO:0048765 GO:0048766 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0055044 GO:0065007 GO:0071695 GO:0071840 GO:0071944 GO:0072583 GO:0090558 GO:0090627 GO:0097305 GO:0097708 GO:0098588 GO:0098657 GO:0098805 GO:0099402 GO:1901700 GO:1905392 GO:2000114
KEGG Pathways
Metabolic & Signaling
ko04072 ko04144 ko04721 ko04961 ko05100 map04072 map04144 map04721 map04961 map05100
Pfam Domains
Protein Families
Dynamin_M Dynamin_N GED PH

Protein Analysis

794

Amino Acids

86.28

Weight (kDa)

8.86

Isoelectric Point (pI)

46.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dynamin_N PF00350 42 - 205 1.4e-30 Dynamin family
Dynamin_M PF01031 257 - 494 7.9e-24 Dynamin central region
PH PF00169 578 - 697 4.6e-11 PH domain
PH_8 PF15409 580 - 631 4.6e-06 Pleckstrin homology domain
GED PF02212 736 - 785 3.1e-06 Dynamin GTPase effector domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012009)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10290 AT1G59610
fragaria_vesca FvH4_2g00460 FvH4_2g00460
malus_domestica MD05G1001600.v1.1 MD10G1002800.v1.1
prunus_persica Prupe.8G004400_v2.0.a1
pyrus_communis pycom05g00170 pycom10g00220
rosa_chinensis RchiOBHm_Chr6g0250411
rosa_laevigata RLG00000015541
rosa_multiflora Rmu_sc0001209.1_g000036
rosa_roxburghii Rroxscaffold_7G00217250
rosa_rugosa Rorug05G0496400 Rorug05G0496400
rosa_samantha Rh6AG005800 Rh6DG003800
rosa_wichuraiana Rw6G000640 Rw6G000710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 1700, 1724, 2200
Acc65I GGTACC 2 cut(s) 587, 998
AccB1I GGYRCC 3 cut(s) 50, 587, 998
AccB7I CCANNNNNTGG 1 cut(s) 1349
AciI CCGC 5 cut(s) 43, 54, 149, 780, 2133
AclWI GGATC 2 cut(s) 458, 999
AcoI YGGCCR 1 cut(s) 1383
AcsI RAATTY 1 cut(s) 1108
AcuI CTGAAG 6 cut(s) 555, 615, 827, 1737, 1875, 2148
AfaI GTAC 5 cut(s) 403, 589, 1000, 1296, 1683
AfiI CCNNNNNNNGG 8 cut(s) 102, 786, 1015, 1222, 1223, 1349, 1607, 2102
AflIII ACRYGT 1 cut(s) 2325
AhdI GACNNNNNGTC 2 cut(s) 1710, 1936
AhlI ACTAGT 2 cut(s) 1595, 2107
AjiI CACGTC 1 cut(s) 1277
AjnI CCWGG 5 cut(s) 433, 657, 782, 1305, 1342
AjuI GAANNNNNNNTTGG 4 cut(s) 901, 933, 1470, 1502
AleI CACNNNNGTG 1 cut(s) 1848
AloI GAACNNNNNNTCC 2 cut(s) 77, 109
Alw21I GWGCWC 4 cut(s) 226, 489, 829, 1925
Alw26I GTCTC 4 cut(s) 942, 1834, 2036, 2229
AlwI GGATC 2 cut(s) 458, 999
AlwNI CAGNNNCTG 2 cut(s) 1715, 2045
Ama87I CYCGRG 2 cut(s) 219, 549
AoxI GGCC 2 cut(s) 1383, 2313
ApeKI GCWGC 8 cut(s) 25, 264, 626, 1325, 1328, 1397, 1516, 2270
ApoI RAATTY 1 cut(s) 1108
ArsI GACNNNNNNTTYG 2 cut(s) 1133, 1165
AseI ATTAAT 1 cut(s) 381
Asp718I GGTACC 2 cut(s) 587, 998
AspLEI GCGC 2 cut(s) 2017, 2024
AspS9I GGNCC 4 cut(s) 680, 1712, 1946, 2135
AsuHPI GGTGA 3 cut(s) 215, 1079, 1950
AsuII TTCGAA 1 cut(s) 1550
AvaI CYCGRG 2 cut(s) 219, 549
AvaII GGWCC 4 cut(s) 680, 1712, 1946, 2135
AxyI CCTNAGG 2 cut(s) 2202, 2283
BalI TGGCCA 1 cut(s) 1385
BanI GGYRCC 3 cut(s) 50, 587, 998
BanII GRGCYC 4 cut(s) 226, 556, 829, 1925
BbsI GAAGAC 3 cut(s) 1709, 2194, 2328
Bbv12I GWGCWC 4 cut(s) 226, 489, 829, 1925
BbvCI CCTCAGC 1 cut(s) 993
BbvI GCAGC 8 cut(s) 37, 276, 613, 1312, 1315, 1384, 1528, 2257
BceAI ACGGC 5 cut(s) 341, 873, 1011, 1687, 2009
BciT130I CCWGG 5 cut(s) 435, 659, 784, 1307, 1344
BclI TGATCA 1 cut(s) 442
BcoDI GTCTC 4 cut(s) 942, 1834, 2036, 2229
BcuI ACTAGT 2 cut(s) 1595, 2107
BfaI CTAG 7 cut(s) 773, 842, 1010, 1596, 1983, 2060, 2108
BfmI CTRYAG 2 cut(s) 651, 1707
BfoI RGCGCY 1 cut(s) 2025
BfuAI ACCTGC 3 cut(s) 1700, 1724, 2200
BglI GCCNNNNNGGC 1 cut(s) 788
BisI GCNGC 9 cut(s) 26, 44, 265, 627, 1326, 1329, 1398, 1517, 2271
BlpI GCTNAGC 1 cut(s) 483
BlsI GCNGC 9 cut(s) 27, 45, 266, 628, 1327, 1330, 1399, 1518, 2272
Bme1390I CCNGG 5 cut(s) 435, 659, 784, 1307, 1344
Bme18I GGWCC 4 cut(s) 680, 1712, 1946, 2135
BmeRI GACNNNNNGTC 2 cut(s) 1710, 1936
BmeT110I CYCGRG 2 cut(s) 219, 549
BmgBI CACGTC 1 cut(s) 1277
BmgT120I GGNCC 4 cut(s) 680, 1712, 1946, 2135
BmiI GGNNCC 5 cut(s) 52, 256, 589, 1000, 1269
BmrFI CCNGG 5 cut(s) 435, 659, 784, 1307, 1344
BmrI ACTGGG 1 cut(s) 1956
BmsI GCATC 7 cut(s) 30, 530, 568, 701, 853, 983, 1396
BmuI ACTGGG 1 cut(s) 1956
BpiI GAAGAC 3 cut(s) 1709, 2194, 2328
BpmI CTGGAG 2 cut(s) 774, 805
Bpu10I CCTNAGC 1 cut(s) 993
Bpu1102I GCTNAGC 1 cut(s) 483
Bpu14I TTCGAA 1 cut(s) 1550
BpuEI CTTGAG 3 cut(s) 280, 510, 2211
Bsa29I ATCGAT 1 cut(s) 1543
BsaBI GATNNNNATC 1 cut(s) 1194
BsaJI CCNNGG 4 cut(s) 434, 704, 1849, 2316
BsaWI WCCGGW 2 cut(s) 821, 2214
BsaXI ACNNNNNCTCC 6 cut(s) 77, 107, 208, 238, 811, 841
Bsc4I CCNNNNNNNGG 8 cut(s) 102, 786, 1015, 1222, 1223, 1349, 1607, 2102
Bse1I ACTGG 6 cut(s) 111, 205, 1069, 1699, 1810, 1962
Bse21I CCTNAGG 2 cut(s) 2202, 2283
Bse3DI GCAATG 2 cut(s) 145, 1386
Bse8I GATNNNNATC 1 cut(s) 1194
BseBI CCWGG 5 cut(s) 435, 659, 784, 1307, 1344
BseCI ATCGAT 1 cut(s) 1543
BseDI CCNNGG 4 cut(s) 434, 704, 1849, 2316
BseJI GATNNNNATC 1 cut(s) 1194
BseLI CCNNNNNNNGG 8 cut(s) 102, 786, 1015, 1222, 1223, 1349, 1607, 2102
BseMI GCAATG 2 cut(s) 145, 1386
BseMII CTCAG 5 cut(s) 314, 474, 571, 984, 2193
BseNI ACTGG 6 cut(s) 111, 205, 1069, 1699, 1810, 1962
BseRI GAGGAG 4 cut(s) 85, 819, 1850, 1883
BseXI GCAGC 8 cut(s) 37, 276, 613, 1312, 1315, 1384, 1528, 2257
BseYI CCCAGC 1 cut(s) 346
BsgI GTGCAG 2 cut(s) 729, 1004
Bsh1285I CGRYCG 1 cut(s) 12
BshFI GGCC 2 cut(s) 1385, 2315
BshNI GGYRCC 3 cut(s) 50, 587, 998
BshVI ATCGAT 1 cut(s) 1543
BsiEI CGRYCG 1 cut(s) 12
BsiHKAI GWGCWC 4 cut(s) 226, 489, 829, 1925
BsiHKCI CYCGRG 2 cut(s) 219, 549
BsiSI CCGG 2 cut(s) 822, 2215
BslFI GGGAC 1 cut(s) 2264
BslI CCNNNNNNNGG 8 cut(s) 102, 786, 1015, 1222, 1223, 1349, 1607, 2102
BsmAI GTCTC 4 cut(s) 942, 1834, 2036, 2229
BsmFI GGGAC 1 cut(s) 2264
BsmI GAATGC 1 cut(s) 328
BsnI GGCC 2 cut(s) 1385, 2315
BsoBI CYCGRG 2 cut(s) 219, 549
Bsp119I TTCGAA 1 cut(s) 1550
Bsp1286I GDGCHC 5 cut(s) 226, 489, 556, 829, 1925
Bsp143I GATC 6 cut(s) 9, 442, 450, 634, 1004, 1544
Bsp1720I GCTNAGC 1 cut(s) 483
BspACI CCGC 5 cut(s) 43, 54, 149, 780, 2133
BspANI GGCC 2 cut(s) 1385, 2315
BspCNI CTCAG 5 cut(s) 313, 475, 570, 985, 2194
BspDI ATCGAT 1 cut(s) 1543
BspLI GGNNCC 5 cut(s) 52, 256, 589, 1000, 1269
BspMAI CTGCAG 2 cut(s) 655, 1711
BspMI ACCTGC 3 cut(s) 1700, 1724, 2200
BspPI GGATC 2 cut(s) 458, 999
BspQI GCTCTTC 2 cut(s) 669, 1913
BspT104I TTCGAA 1 cut(s) 1550
BspT107I GGYRCC 3 cut(s) 50, 587, 998
BsrDI GCAATG 2 cut(s) 145, 1386
BsrI ACTGG 6 cut(s) 111, 205, 1069, 1699, 1810, 1962
BssECI CCNNGG 4 cut(s) 434, 704, 1849, 2316
BssMI GATC 6 cut(s) 9, 442, 450, 634, 1004, 1544
BssT1I CCWWGG 2 cut(s) 704, 2316
Bst2UI CCWGG 5 cut(s) 435, 659, 784, 1307, 1344
Bst4CI ACNGT 5 cut(s) 173, 598, 750, 2005, 2047
Bst6I CTCTTC 4 cut(s) 669, 1163, 1878, 1913
BstAPI GCANNNNNTGC 4 cut(s) 334, 650, 1397, 1406
BstBI TTCGAA 1 cut(s) 1550
BstC8I GCNNGC 2 cut(s) 1260, 1778
BstDEI CTNAG 7 cut(s) 300, 483, 557, 993, 1252, 2202, 2283
BstDSI CCRYGG 1 cut(s) 1849
BstENI CCTNNNNNAGG 3 cut(s) 100, 1605, 2100
BstH2I RGCGCY 1 cut(s) 2025
BstHHI GCGC 2 cut(s) 2017, 2024
BstKTI GATC 6 cut(s) 12, 445, 453, 637, 1007, 1547
BstMAI GTCTC 4 cut(s) 942, 1834, 2036, 2229
BstMBI GATC 6 cut(s) 9, 442, 450, 634, 1004, 1544
BstMCI CGRYCG 1 cut(s) 12
BstNI CCWGG 5 cut(s) 435, 659, 784, 1307, 1344
BstNSI RCATGY 1 cut(s) 2329
BstSCI CCNGG 5 cut(s) 433, 657, 782, 1305, 1342
BstSFI CTRYAG 2 cut(s) 651, 1707
BstV1I GCAGC 8 cut(s) 37, 276, 613, 1312, 1315, 1384, 1528, 2257
BstV2I GAAGAC 3 cut(s) 1709, 2194, 2328
BstX2I RGATCY 1 cut(s) 1004
BstYI RGATCY 1 cut(s) 1004
Bsu15I ATCGAT 1 cut(s) 1543
Bsu36I CCTNAGG 2 cut(s) 2202, 2283
BsuRI GGCC 2 cut(s) 1385, 2315
BsuTUI ATCGAT 1 cut(s) 1543
BtgI CCRYGG 1 cut(s) 1849
BtgZI GCGATG 1 cut(s) 1795
BtrI CACGTC 1 cut(s) 1277
BveI ACCTGC 3 cut(s) 1700, 1724, 2200
Cac8I GCNNGC 2 cut(s) 1260, 1778
CaiI CAGNNNCTG 2 cut(s) 1715, 2045
CfoI GCGC 2 cut(s) 2017, 2024
Cfr13I GGNCC 4 cut(s) 680, 1712, 1946, 2135
ClaI ATCGAT 1 cut(s) 1543
Csp6I GTAC 5 cut(s) 402, 588, 999, 1295, 1682
CviAII CATG 3 cut(s) 454, 1633, 2326
CviQI GTAC 5 cut(s) 402, 588, 999, 1295, 1682
DdeI CTNAG 7 cut(s) 300, 483, 557, 993, 1252, 2202, 2283
DpnI GATC 6 cut(s) 11, 444, 452, 636, 1006, 1546
DpnII GATC 6 cut(s) 9, 442, 450, 634, 1004, 1544
DriI GACNNNNNGTC 2 cut(s) 1710, 1936
EaeI YGGCCR 1 cut(s) 1383
Eam1104I CTCTTC 4 cut(s) 669, 1163, 1878, 1913
Eam1105I GACNNNNNGTC 2 cut(s) 1710, 1936
EarI CTCTTC 4 cut(s) 669, 1163, 1878, 1913
Ecl136II GAGCTC 3 cut(s) 224, 827, 1923
Eco130I CCWWGG 2 cut(s) 704, 2316
Eco24I GRGCYC 4 cut(s) 226, 556, 829, 1925
Eco32I GATATC 2 cut(s) 700, 1156
Eco47I GGWCC 4 cut(s) 680, 1712, 1946, 2135
Eco53kI GAGCTC 3 cut(s) 224, 827, 1923
Eco57I CTGAAG 6 cut(s) 555, 615, 827, 1737, 1875, 2148
Eco81I CCTNAGG 2 cut(s) 2202, 2283
Eco88I CYCGRG 2 cut(s) 219, 549
EcoICRI GAGCTC 3 cut(s) 224, 827, 1923
EcoNI CCTNNNNNAGG 3 cut(s) 100, 1605, 2100
EcoO109I RGGNCCY 1 cut(s) 1712
EcoRII CCWGG 5 cut(s) 433, 657, 782, 1305, 1342
EcoRV GATATC 2 cut(s) 700, 1156
EcoT14I CCWWGG 2 cut(s) 704, 2316
EcoT38I GRGCYC 4 cut(s) 226, 556, 829, 1925
ErhI CCWWGG 2 cut(s) 704, 2316
FaeI CATG 3 cut(s) 457, 1636, 2329
FalI AAGNNNNNCTT 2 cut(s) 888, 920
FaqI GGGAC 1 cut(s) 2264
FatI CATG 3 cut(s) 453, 1632, 2325
FauI CCCGC 1 cut(s) 142
FbaI TGATCA 1 cut(s) 442
Fnu4HI GCNGC 9 cut(s) 26, 44, 265, 627, 1326, 1329, 1398, 1517, 2271
FriOI GRGCYC 4 cut(s) 226, 556, 829, 1925
Fsp4HI GCNGC 9 cut(s) 26, 44, 265, 627, 1326, 1329, 1398, 1517, 2271
FspBI CTAG 7 cut(s) 773, 842, 1010, 1596, 1983, 2060, 2108
GlaI GCGC 2 cut(s) 2016, 2023
GluI GCNGC 9 cut(s) 26, 44, 265, 627, 1326, 1329, 1398, 1517, 2271
GsaI CCCAGC 1 cut(s) 350
GsuI CTGGAG 2 cut(s) 774, 805
HaeII RGCGCY 1 cut(s) 2025
HaeIII GGCC 2 cut(s) 1385, 2315
HapII CCGG 2 cut(s) 822, 2215
HhaI GCGC 2 cut(s) 2017, 2024
Hin1II CATG 3 cut(s) 457, 1636, 2329
Hin6I GCGC 2 cut(s) 2015, 2022
HinP1I GCGC 2 cut(s) 2015, 2022
HindIII AAGCTT 2 cut(s) 965, 1811
HinfI GANTC 7 cut(s) 35, 461, 494, 561, 880, 1585, 2182
HpaII CCGG 2 cut(s) 822, 2215
HphI GGTGA 3 cut(s) 215, 1079, 1950
Hpy166II GTNNAC 1 cut(s) 79
Hpy8I GTNNAC 1 cut(s) 79
Hpy99I CGWCG 3 cut(s) 71, 128, 131
HpyAV CCTTC 5 cut(s) 1097, 1639, 1712, 2106, 2123
HpyCH4III ACNGT 5 cut(s) 173, 598, 750, 2005, 2047
HpyCH4IV ACGT 3 cut(s) 123, 1276, 1459
HpyF3I CTNAG 7 cut(s) 300, 483, 557, 993, 1252, 2202, 2283
HpySE526I ACGT 3 cut(s) 123, 1276, 1459
Hsp92II CATG 3 cut(s) 457, 1636, 2329
HspAI GCGC 2 cut(s) 2015, 2022
KpnI GGTACC 2 cut(s) 591, 1002
Ksp22I TGATCA 1 cut(s) 442
Kzo9I GATC 6 cut(s) 9, 442, 450, 634, 1004, 1544
LguI GCTCTTC 2 cut(s) 669, 1913
LmnI GCTCC 6 cut(s) 229, 824, 832, 1018, 1267, 1773
Lsp1109I GCAGC 8 cut(s) 37, 276, 613, 1312, 1315, 1384, 1528, 2257
LweI GCATC 7 cut(s) 30, 530, 568, 701, 853, 983, 1396
MaeI CTAG 7 cut(s) 773, 842, 1010, 1596, 1983, 2060, 2108
MaeII ACGT 3 cut(s) 123, 1276, 1459
MaeIII GTNAC 2 cut(s) 924, 1817
MalI GATC 6 cut(s) 11, 444, 452, 636, 1006, 1546
MboI GATC 6 cut(s) 9, 442, 450, 634, 1004, 1544
MflI RGATCY 1 cut(s) 1004
MhlI GDGCHC 5 cut(s) 226, 489, 556, 829, 1925
MlsI TGGCCA 1 cut(s) 1385
MluCI AATT 7 cut(s) 17, 378, 382, 419, 498, 1086, 1108
MluNI TGGCCA 1 cut(s) 1385
MlyI GAGTC 4 cut(s) 29, 470, 570, 2176
MmeI TCCRAC 2 cut(s) 1075, 1161
Mox20I TGGCCA 1 cut(s) 1385
MscI TGGCCA 1 cut(s) 1385
MseI TTAA 8 cut(s) 120, 381, 671, 716, 1238, 1413, 1974, 2330
MslI CAYNNNNRTG 1 cut(s) 1848
Msp20I TGGCCA 1 cut(s) 1385
MspI CCGG 2 cut(s) 822, 2215
MspR9I CCNGG 5 cut(s) 435, 659, 784, 1307, 1344
Mva1269I GAATGC 1 cut(s) 328
MvaI CCWGG 5 cut(s) 435, 659, 784, 1307, 1344
NdeII GATC 6 cut(s) 9, 442, 450, 634, 1004, 1544
NlaIII CATG 3 cut(s) 457, 1636, 2329
NlaIV GGNNCC 5 cut(s) 52, 256, 589, 1000, 1269
NmeAIII GCCGAG 1 cut(s) 114
NspI RCATGY 1 cut(s) 2329
NspV TTCGAA 1 cut(s) 1550
OliI CACNNNNGTG 1 cut(s) 1848
PaeR7I CTCGAG 2 cut(s) 219, 549
PciI ACATGT 1 cut(s) 2325
PciSI GCTCTTC 2 cut(s) 669, 1913
PctI GAATGC 1 cut(s) 328
PfeI GAWTC 3 cut(s) 494, 880, 1585
PflFI GACNNNGTC 1 cut(s) 2151
PflMI CCANNNNNTGG 1 cut(s) 1349
PkrI GCNGC 9 cut(s) 27, 45, 266, 628, 1327, 1330, 1399, 1518, 2272
Ple19I CGATCG 1 cut(s) 12
PleI GAGTC 4 cut(s) 29, 469, 569, 2176
PpsI GAGTC 4 cut(s) 29, 469, 569, 2176
PpuMI RGGWCCY 1 cut(s) 1712
PscI ACATGT 1 cut(s) 2325
PshBI ATTAAT 1 cut(s) 381
Psp124BI GAGCTC 3 cut(s) 226, 829, 1925
Psp5II RGGWCCY 1 cut(s) 1712
Psp6I CCWGG 5 cut(s) 433, 657, 782, 1305, 1342
PspFI CCCAGC 1 cut(s) 346
PspGI CCWGG 5 cut(s) 433, 657, 782, 1305, 1342
PspN4I GGNNCC 5 cut(s) 52, 256, 589, 1000, 1269
PspPI GGNCC 4 cut(s) 680, 1712, 1946, 2135
PspPPI RGGWCCY 1 cut(s) 1712
PspXI VCTCGAGB 1 cut(s) 219
PstI CTGCAG 2 cut(s) 655, 1711
PstNI CAGNNNCTG 2 cut(s) 1715, 2045
PsuI RGATCY 1 cut(s) 1004
PsyI GACNNNGTC 1 cut(s) 2151
PvuI CGATCG 1 cut(s) 12
RsaI GTAC 5 cut(s) 403, 589, 1000, 1296, 1683
RsaNI GTAC 5 cut(s) 402, 588, 999, 1295, 1682
RseI CAYNNNNRTG 1 cut(s) 1848
SacI GAGCTC 3 cut(s) 226, 829, 1925
SapI GCTCTTC 2 cut(s) 669, 1913
SaqAI TTAA 8 cut(s) 120, 381, 671, 716, 1238, 1413, 1974, 2330
SatI GCNGC 9 cut(s) 26, 44, 265, 627, 1326, 1329, 1398, 1517, 2271
Sau3AI GATC 6 cut(s) 9, 442, 450, 634, 1004, 1544
Sau96I GGNCC 4 cut(s) 680, 1712, 1946, 2135
SchI GAGTC 4 cut(s) 29, 470, 570, 2176
ScrFI CCNGG 5 cut(s) 435, 659, 784, 1307, 1344
SduI GDGCHC 5 cut(s) 226, 489, 556, 829, 1925
SfaNI GCATC 7 cut(s) 30, 530, 568, 701, 853, 983, 1396
SfcI CTRYAG 2 cut(s) 651, 1707
Sfr274I CTCGAG 2 cut(s) 219, 549
SfuI TTCGAA 1 cut(s) 1550
SinI GGWCC 4 cut(s) 680, 1712, 1946, 2135
SlaI CTCGAG 2 cut(s) 219, 549
SmiMI CAYNNNNRTG 1 cut(s) 1848
SmlI CTYRAG 5 cut(s) 219, 259, 525, 549, 2226
SmoI CTYRAG 5 cut(s) 219, 259, 525, 549, 2226
SpeI ACTAGT 2 cut(s) 1595, 2107
Sse9I AATT 7 cut(s) 17, 378, 382, 419, 498, 1086, 1108
SsiI CCGC 5 cut(s) 43, 54, 149, 780, 2133
SspI AATATT 1 cut(s) 1879
SspMI CTAG 7 cut(s) 773, 842, 1010, 1596, 1983, 2060, 2108
SstI GAGCTC 3 cut(s) 226, 829, 1925
StyD4I CCNGG 5 cut(s) 433, 657, 782, 1305, 1342
StyI CCWWGG 2 cut(s) 704, 2316
TaaI ACNGT 5 cut(s) 173, 598, 750, 2005, 2047
TaiI ACGT 3 cut(s) 126, 1279, 1462
TaqI TCGA 9 cut(s) 12, 38, 220, 550, 901, 1543, 1550, 1916, 2185
TaqII GACCGA 1 cut(s) 1158
TasI AATT 7 cut(s) 17, 378, 382, 419, 498, 1086, 1108
TatI WGTACW 2 cut(s) 401, 1681
TauI GCSGC 1 cut(s) 46
TfiI GAWTC 3 cut(s) 494, 880, 1585
Tru1I TTAA 8 cut(s) 120, 381, 671, 716, 1238, 1413, 1974, 2330
Tru9I TTAA 8 cut(s) 120, 381, 671, 716, 1238, 1413, 1974, 2330
TseI GCWGC 8 cut(s) 25, 264, 626, 1325, 1328, 1397, 1516, 2270
TspGWI ACGGA 1 cut(s) 1838
Tth111I GACNNNGTC 1 cut(s) 2151
Van91I CCANNNNNTGG 1 cut(s) 1349
VpaK11BI GGWCC 4 cut(s) 680, 1712, 1946, 2135
VspI ATTAAT 1 cut(s) 381
XagI CCTNNNNNAGG 3 cut(s) 100, 1605, 2100
XapI RAATTY 1 cut(s) 1108
XbaI TCTAGA 1 cut(s) 772
XceI RCATGY 1 cut(s) 2329
XhoI CTCGAG 2 cut(s) 219, 549
XspI CTAG 7 cut(s) 773, 842, 1010, 1596, 1983, 2060, 2108
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.