pycom05g04460

ribosome-binding factor A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0012528
Physical Location & Seq
Forward (+)
5244729 .. 5247939
3211 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 888 bp
ATGGTTAATATCAAGTTCCGATTGTTCCTCCCGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAACAAAACAATATCCTTCTCTTGTTCTTGCCTCCTGCAACTTTGCATTCCCTCGCTCTCGACACAAAGCAAATTCTTGAAAGTAAGAATTCAGGACACAAACAACAAAATAAAATCAAAGAGGAGAAGAGAAGAGCGAGCTGGGATAGAATACAGCAATCAGCAATGCCAAACTTAGTCCTCCACCACCCACCATTCACCCCCTTCCTTTCCCACCTCCCATCATCATCATCAAGCTTTCCAATTCGGACCCAGAAATCAACGGCCTCCTTTCCCCCAATAGATCATGCGGTTCTGCTCCGTTGGCGGCCCAACACCATCAGGTGCATGGCGAACCCGCGCAGAGTAGCAATGGTGGCTAAGCAGATAAGGAGGGAGCTTTCTGACATGCTCTTAACTGATAAGGTCTTGCAGTACGCTATTCTTCCTGAGGTTTCTTTGGGTGCTGACCGTTACCTCTCTTCGCTAACCACCATAAGCGATGTTGAAGTCTCCACCGACTTGCAGGTGGTTAAAGTCTATGTTTCTATTTTTGGCGATGAAAGAGGGAAAGAGGTTGCCCTTGCTGGGCTAAAGTCAAAAGCCAAATATGTCCGAGGTCAATTAGGAAGGCGTATGAAGCTGCGGTTAACTCCTGAGATACGCTTCATAGAAGATGAGTCTCTAGAGAGAGGAAGCAGGGTGATTGCTATATTAGATAAGATAAAGAATGAAAAGAAAGATTCAGAAATTCAAGATGACGAACAATTGGAAACATCCGAGGCACCTGAGGATGACGGAGATTGGGAGGGTGATGACCCTGATGAAGACATTATATACATAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008150 GO:0009507 GO:0009536 GO:0022613 GO:0042254 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044085 GO:0044424 GO:0044444 GO:0044464 GO:0071840
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RBFA

Protein Analysis

296

Amino Acids

33.85

Weight (kDa)

9.43

Isoelectric Point (pI)

58.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RBFA PF02033 135 - 251 7.2e-26 Ribosome-binding factor A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012528)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34730 AT4G34730 AT4G34730
fragaria_vesca FvH4_2g10740 FvH4_2g10740
malus_domestica MD00G1066800.v1.1
prunus_persica Prupe.8G082300_v2.0.a1
pyrus_communis pycom05g04460 pycom10g04740
rosa_chinensis RchiOBHm_Chr6g0268531
rosa_laevigata RLG00000013905
rosa_multiflora Rmu_sc0012389.1_g000002
rosa_roxburghii Rroxscaffold_7G00199750
rosa_rugosa Rorug06G0042900
rosa_samantha Rh6AG162300 Rh6BG166500 Rh6CG160300 Rh6DG151700
rosa_wichuraiana Rw6G014010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 556
Acc36I ACCTGC 1 cut(s) 556
AccB1I GGYRCC 1 cut(s) 823
AccII CGCG 1 cut(s) 400
AciI CCGC 4 cut(s) 350, 367, 398, 685
AcsI RAATTY 3 cut(s) 132, 148, 789
AfaI GTAC 1 cut(s) 476
AfiI CCNNNNNNNGG 1 cut(s) 627
AgsI TTSAA 3 cut(s) 140, 548, 794
AluBI AGCT 4 cut(s) 201, 297, 439, 682
AluI AGCT 4 cut(s) 201, 297, 439, 682
Alw26I GTCTC 2 cut(s) 556, 726
Ama87I CYCGRG 1 cut(s) 31
AoxI GGCC 2 cut(s) 324, 368
ApeKI GCWGC 1 cut(s) 682
ApoI RAATTY 3 cut(s) 132, 148, 789
AspLEI GCGC 1 cut(s) 402
AspS9I GGNCC 2 cut(s) 309, 369
AsuHPI GGTGA 3 cut(s) 250, 754, 863
AvaI CYCGRG 1 cut(s) 31
AvaII GGWCC 1 cut(s) 309
AxyI CCTNAGG 2 cut(s) 489, 828
BanI GGYRCC 1 cut(s) 823
BbsI GAAGAC 1 cut(s) 873
BbvI GCAGC 1 cut(s) 669
BccI CCATC 2 cut(s) 289, 386
BceAI ACGGC 1 cut(s) 339
BcoDI GTCTC 2 cut(s) 556, 726
BfaI CTAG 1 cut(s) 725
BfuAI ACCTGC 1 cut(s) 556
BisI GCNGC 2 cut(s) 368, 683
BlpI GCTNAGC 1 cut(s) 420
BlsI GCNGC 2 cut(s) 369, 684
Bme18I GGWCC 1 cut(s) 309
BmeT110I CYCGRG 1 cut(s) 31
BmgT120I GGNCC 2 cut(s) 309, 369
BmiI GGNNCC 2 cut(s) 311, 825
BpiI GAAGAC 1 cut(s) 873
Bpu1102I GCTNAGC 1 cut(s) 420
BsaJI CCNNGG 2 cut(s) 655, 819
Bsc4I CCNNNNNNNGG 1 cut(s) 627
Bse21I CCTNAGG 2 cut(s) 489, 828
Bse3DI GCAATG 2 cut(s) 231, 417
BseDI CCNNGG 2 cut(s) 655, 819
BseGI GGATG 2 cut(s) 815, 838
BseLI CCNNNNNNNGG 1 cut(s) 627
BseMI GCAATG 2 cut(s) 231, 417
BseMII CTCAG 3 cut(s) 480, 687, 819
BseRI GAGGAG 1 cut(s) 197
BseXI GCAGC 1 cut(s) 669
BseYI CCCAGC 2 cut(s) 201, 626
Bsh1236I CGCG 1 cut(s) 400
BshFI GGCC 2 cut(s) 326, 370
BshNI GGYRCC 1 cut(s) 823
BsiHKCI CYCGRG 1 cut(s) 31
BslI CCNNNNNNNGG 1 cut(s) 627
BsmAI GTCTC 2 cut(s) 556, 726
BsmI GAATGC 1 cut(s) 106
BsnI GGCC 2 cut(s) 326, 370
BsoBI CYCGRG 1 cut(s) 31
Bsp143I GATC 1 cut(s) 343
Bsp1720I GCTNAGC 1 cut(s) 420
BspACI CCGC 4 cut(s) 350, 367, 398, 685
BspANI GGCC 2 cut(s) 326, 370
BspCNI CTCAG 3 cut(s) 481, 688, 820
BspFNI CGCG 1 cut(s) 400
BspLI GGNNCC 2 cut(s) 311, 825
BspMI ACCTGC 1 cut(s) 556
BspQI GCTCTTC 1 cut(s) 187
BspT107I GGYRCC 1 cut(s) 823
BsrDI GCAATG 2 cut(s) 231, 417
BssECI CCNNGG 2 cut(s) 655, 819
BssMI GATC 1 cut(s) 343
Bst4CI ACNGT 1 cut(s) 512
Bst6I CTCTTC 3 cut(s) 182, 187, 526
BstC8I GCNNGC 1 cut(s) 199
BstDEI CTNAG 5 cut(s) 235, 420, 489, 696, 828
BstF5I GGATG 2 cut(s) 815, 838
BstFNI CGCG 1 cut(s) 400
BstHHI GCGC 1 cut(s) 402
BstKTI GATC 1 cut(s) 346
BstMAI GTCTC 2 cut(s) 556, 726
BstMBI GATC 1 cut(s) 343
BstMWI GCNNNNNNNGC 3 cut(s) 364, 416, 679
BstNSI RCATGY 1 cut(s) 451
BstUI CGCG 1 cut(s) 400
BstV1I GCAGC 1 cut(s) 669
BstV2I GAAGAC 1 cut(s) 873
Bsu36I CCTNAGG 2 cut(s) 489, 828
BsuRI GGCC 2 cut(s) 326, 370
BtgZI GCGATG 2 cut(s) 555, 612
BtsCI GGATG 2 cut(s) 815, 838
BveI ACCTGC 1 cut(s) 556
Cac8I GCNNGC 1 cut(s) 199
CfoI GCGC 1 cut(s) 402
Cfr13I GGNCC 2 cut(s) 309, 369
Csp6I GTAC 1 cut(s) 475
CviAII CATG 3 cut(s) 347, 388, 448
CviJI RGCY 9 cut(s) 201, 297, 326, 370, 419, 439, 631, 644, 682
CviKI_1 RGCY 9 cut(s) 201, 297, 326, 370, 419, 439, 631, 644, 682
CviQI GTAC 1 cut(s) 475
DdeI CTNAG 5 cut(s) 235, 420, 489, 696, 828
DpnI GATC 1 cut(s) 345
DpnII GATC 1 cut(s) 343
Eam1104I CTCTTC 3 cut(s) 182, 187, 526
EarI CTCTTC 3 cut(s) 182, 187, 526
Eco47I GGWCC 1 cut(s) 309
Eco81I CCTNAGG 2 cut(s) 489, 828
Eco88I CYCGRG 1 cut(s) 31
EcoRI GAATTC 1 cut(s) 148
FaeI CATG 3 cut(s) 350, 391, 451
FatI CATG 3 cut(s) 346, 387, 447
FauI CCCGC 1 cut(s) 405
Fnu4HI GCNGC 2 cut(s) 368, 683
FokI GGATG 2 cut(s) 802, 845
Fsp4HI GCNGC 2 cut(s) 368, 683
FspBI CTAG 1 cut(s) 725
GlaI GCGC 1 cut(s) 401
GluI GCNGC 2 cut(s) 368, 683
GsaI CCCAGC 2 cut(s) 205, 630
HaeIII GGCC 2 cut(s) 326, 370
HhaI GCGC 1 cut(s) 402
Hin1II CATG 3 cut(s) 350, 391, 451
Hin6I GCGC 1 cut(s) 400
HinP1I GCGC 1 cut(s) 400
HincII GTYRAC 1 cut(s) 690
HindII GTYRAC 1 cut(s) 690
HindIII AAGCTT 1 cut(s) 295
HinfI GANTC 2 cut(s) 719, 782
HpaI GTTAAC 1 cut(s) 690
HphI GGTGA 3 cut(s) 250, 754, 863
Hpy166II GTNNAC 1 cut(s) 690
Hpy188I TCNGA 6 cut(s) 20, 309, 445, 656, 787, 820
Hpy188III TCNNGA 8 cut(s) 31, 119, 137, 153, 488, 695, 725, 794
Hpy8I GTNNAC 1 cut(s) 690
HpyAV CCTTC 3 cut(s) 86, 274, 663
HpyCH4III ACNGT 1 cut(s) 512
HpyCH4V TGCA 5 cut(s) 98, 106, 387, 472, 565
HpyF10VI GCNNNNNNNGC 3 cut(s) 364, 416, 679
HpyF3I CTNAG 5 cut(s) 235, 420, 489, 696, 828
Hsp92II CATG 3 cut(s) 350, 391, 451
HspAI GCGC 1 cut(s) 400
KspAI GTTAAC 1 cut(s) 690
Kzo9I GATC 1 cut(s) 343
LguI GCTCTTC 1 cut(s) 187
LmnI GCTCC 2 cut(s) 363, 436
Lsp1109I GCAGC 1 cut(s) 669
MaeI CTAG 1 cut(s) 725
MaeIII GTNAC 1 cut(s) 512
MalI GATC 1 cut(s) 345
MboI GATC 1 cut(s) 343
MboII GAAGA 6 cut(s) 199, 204, 476, 513, 725, 878
MfeI CAATTG 1 cut(s) 806
MluCI AATT 6 cut(s) 132, 148, 303, 662, 789, 806
MlyI GAGTC 1 cut(s) 728
MseI TTAA 4 cut(s) 6, 455, 573, 689
MunI CAATTG 1 cut(s) 806
Mva1269I GAATGC 1 cut(s) 106
MvnI CGCG 1 cut(s) 400
MwoI GCNNNNNNNGC 3 cut(s) 364, 416, 679
NdeII GATC 1 cut(s) 343
NlaIII CATG 3 cut(s) 350, 391, 451
NlaIV GGNNCC 2 cut(s) 311, 825
NspI RCATGY 1 cut(s) 451
PaqCI CACCTGC 1 cut(s) 556
PciSI GCTCTTC 1 cut(s) 187
PctI GAATGC 1 cut(s) 106
PfeI GAWTC 1 cut(s) 782
PkrI GCNGC 2 cut(s) 369, 684
PleI GAGTC 1 cut(s) 727
PpsI GAGTC 1 cut(s) 727
PspFI CCCAGC 2 cut(s) 201, 626
PspN4I GGNNCC 2 cut(s) 311, 825
PspPI GGNCC 2 cut(s) 309, 369
RsaI GTAC 1 cut(s) 476
RsaNI GTAC 1 cut(s) 475
SapI GCTCTTC 1 cut(s) 187
SaqAI TTAA 4 cut(s) 6, 455, 573, 689
SatI GCNGC 2 cut(s) 368, 683
Sau3AI GATC 1 cut(s) 343
Sau96I GGNCC 2 cut(s) 309, 369
SchI GAGTC 1 cut(s) 728
SinI GGWCC 1 cut(s) 309
Sse9I AATT 6 cut(s) 132, 148, 303, 662, 789, 806
SsiI CCGC 4 cut(s) 350, 367, 398, 685
SspMI CTAG 1 cut(s) 725
TaaI ACNGT 1 cut(s) 512
TaqI TCGA 1 cut(s) 120
TasI AATT 6 cut(s) 132, 148, 303, 662, 789, 806
TauI GCSGC 1 cut(s) 370
TfiI GAWTC 1 cut(s) 782
Tru1I TTAA 4 cut(s) 6, 455, 573, 689
Tru9I TTAA 4 cut(s) 6, 455, 573, 689
TseI GCWGC 1 cut(s) 682
TspDTI ATGAA 5 cut(s) 615, 692, 697, 786, 879
TspGWI ACGGA 2 cut(s) 350, 852
VpaK11BI GGWCC 1 cut(s) 309
XapI RAATTY 3 cut(s) 132, 148, 789
XbaI TCTAGA 1 cut(s) 724
XceI RCATGY 1 cut(s) 451
XcmI CCANNNNNNNNNTGG 1 cut(s) 385
XspI CTAG 1 cut(s) 725
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.