pycom05g06520

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0015534
Physical Location & Seq
Forward (+)
9009059 .. 9010422
1364 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g06520.1

Sequence Viewer

Length: 720 bp
ATGGGGAAGATAACAATGGCGAAGAAGAAGAAGAACACGATCATGGTCACACCATTGATGATGGTAATGATGACGATGTATGTATTTGTTGGAGATGCAGCTGATACAAACAGTGTTTATGATCCATGCTCAGATGCAAAAATCCAGAGATTAGATGGGTTTACTTTTGGCCTTGCATTTTCAAAAAAAGACAAGTTCTTCTTTAATCAGACTCAACTTTCTCCGTGTGATAAGCGGCTTTCGCTCACCGGAAATGATGCCCAGCTTGCTGTGTTTAGGCCTAAAGTTGATGAGATGTCTTTCCTAAGCATCAACAACAGCACCTTCAGTCAGATCAAGGCTGGTGTGTACATGGTAGCTTTTGCCGGACGGAAGTATGCAGCAAGATCACCCCCAATATTGGTTGCTGATGACTCACACACCATAACAAGCTTCACCTTGGTACTTGAATTTGAAAGGGGGACTCTTCTTAATTTGTATTGGAAGAAATTCGGGTGCAAGGCATGCTCCGGGGACTATTCTGTGTGCCTCAACGATGAAGATTGTGCTGTTCCAAATTCAAAATGTCAAGGCAGTGGTGGATCTTTTGACTGCAACCTAAGCATACAGTTGACCTTCTCAGGAACAGACAAACATCTTCAAGTTCTTAACTCCTGGTATGAGGTGAAAAATCTTCGGAAGTACTCACTCTATGCCCTATTCTCCAATCTTCTGCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

240

Amino Acids

26.71

Weight (kDa)

8.82

Isoelectric Point (pI)

27.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015534)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g07220
malus_domestica MD05G1072900.v1.1
prunus_persica Prupe.8G113200_v2.0.a1
pyrus_communis pycom05g06520
rosa_chinensis RchiOBHm_Chr6g0259741
rosa_laevigata RLG00000014512
rosa_multiflora Rmu_sc0002130.1_g000009 Rmu_sc0015614.1_g000002
rosa_roxburghii Rroxscaffold_7G00207000
rosa_rugosa Rorug05G0590300
rosa_samantha Rh6AG106700 Rh6BG102000 Rh6CG095700 Rh6DG089600
rosa_wichuraiana Rw0G013490 Rw6G009240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 235
AclWI GGATC 2 cut(s) 116, 589
AcsI RAATTY 3 cut(s) 449, 488, 556
AcuI CTGAAG 1 cut(s) 310
AfaI GTAC 3 cut(s) 350, 444, 683
AfiI CCNNNNNNNGG 1 cut(s) 400
AgsI TTSAA 5 cut(s) 183, 449, 455, 561, 641
AjnI CCWGG 1 cut(s) 653
AluBI AGCT 4 cut(s) 101, 265, 359, 432
AluI AGCT 4 cut(s) 101, 265, 359, 432
AlwI GGATC 2 cut(s) 116, 589
AoxI GGCC 2 cut(s) 169, 278
ApeKI GCWGC 2 cut(s) 98, 380
ApoI RAATTY 3 cut(s) 449, 488, 556
Asp700I GAANNNNTTC 1 cut(s) 488
AsuC2I CCSGG 1 cut(s) 511
AsuHPI GGTGA 4 cut(s) 238, 381, 427, 676
BbvI GCAGC 2 cut(s) 110, 392
BccI CCATC 2 cut(s) 55, 149
BciT130I CCWGG 1 cut(s) 655
BcnI CCSGG 1 cut(s) 511
BfmI CTRYAG 1 cut(s) 713
BisI GCNGC 3 cut(s) 99, 236, 381
BlsI GCNGC 3 cut(s) 100, 237, 382
BmcAI AGTACT 1 cut(s) 683
Bme1390I CCNGG 2 cut(s) 511, 655
BmrFI CCNGG 2 cut(s) 511, 655
BmsI GCATC 4 cut(s) 85, 124, 247, 318
Bpu10I CCTNAGC 2 cut(s) 305, 599
BpuMI CCSGG 1 cut(s) 511
BsaJI CCNNGG 2 cut(s) 438, 510
BsaWI WCCGGW 1 cut(s) 248
Bsc4I CCNNNNNNNGG 1 cut(s) 400
BseBI CCWGG 1 cut(s) 655
BseDI CCNNGG 2 cut(s) 438, 510
BseLI CCNNNNNNNGG 1 cut(s) 400
BseMII CTCAG 2 cut(s) 144, 633
BseXI GCAGC 2 cut(s) 110, 392
BseYI CCCAGC 1 cut(s) 261
BshFI GGCC 2 cut(s) 171, 280
BsiSI CCGG 3 cut(s) 249, 366, 510
BslFI GGGAC 2 cut(s) 475, 527
BslI CCNNNNNNNGG 1 cut(s) 400
BsmFI GGGAC 2 cut(s) 475, 527
BsnI GGCC 2 cut(s) 171, 280
Bsp1407I TGTACA 1 cut(s) 348
Bsp143I GATC 5 cut(s) 39, 121, 333, 386, 581
BspACI CCGC 1 cut(s) 235
BspANI GGCC 2 cut(s) 171, 280
BspCNI CTCAG 2 cut(s) 143, 632
BspMAI CTGCAG 1 cut(s) 717
BspPI GGATC 2 cut(s) 116, 589
BsrGI TGTACA 1 cut(s) 348
BssECI CCNNGG 2 cut(s) 438, 510
BssMI GATC 5 cut(s) 39, 121, 333, 386, 581
BssT1I CCWWGG 1 cut(s) 438
Bst2UI CCWGG 1 cut(s) 655
Bst4CI ACNGT 2 cut(s) 113, 609
Bst6I CTCTTC 1 cut(s) 471
BstAPI GCANNNNNTGC 1 cut(s) 504
BstAUI TGTACA 1 cut(s) 348
BstC8I GCNNGC 2 cut(s) 267, 505
BstDEI CTNAG 4 cut(s) 130, 305, 599, 619
BstKTI GATC 5 cut(s) 42, 124, 336, 389, 584
BstMBI GATC 5 cut(s) 39, 121, 333, 386, 581
BstMWI GCNNNNNNNGC 4 cut(s) 241, 266, 504, 600
BstNI CCWGG 1 cut(s) 655
BstNSI RCATGY 1 cut(s) 507
BstSCI CCNGG 2 cut(s) 509, 653
BstSFI CTRYAG 1 cut(s) 713
BstV1I GCAGC 2 cut(s) 110, 392
BstX2I RGATCY 1 cut(s) 581
BstYI RGATCY 1 cut(s) 581
BsuRI GGCC 2 cut(s) 171, 280
BtsI GCAGTG 1 cut(s) 580
BtsIMutI CAGTG 2 cut(s) 118, 580
Cac8I GCNNGC 2 cut(s) 267, 505
Csp6I GTAC 3 cut(s) 349, 443, 682
CviAII CATG 4 cut(s) 43, 126, 352, 504
CviJI RGCY 8 cut(s) 101, 171, 238, 265, 280, 341, 359, 432
CviKI_1 RGCY 8 cut(s) 101, 171, 238, 265, 280, 341, 359, 432
CviQI GTAC 3 cut(s) 349, 443, 682
DdeI CTNAG 4 cut(s) 130, 305, 599, 619
DpnI GATC 5 cut(s) 41, 123, 335, 388, 583
DpnII GATC 5 cut(s) 39, 121, 333, 386, 581
Eam1104I CTCTTC 1 cut(s) 471
EarI CTCTTC 1 cut(s) 471
Eco130I CCWWGG 1 cut(s) 438
Eco147I AGGCCT 1 cut(s) 280
Eco57I CTGAAG 1 cut(s) 310
EcoRII CCWGG 1 cut(s) 653
EcoT14I CCWWGG 1 cut(s) 438
ErhI CCWWGG 1 cut(s) 438
FaeI CATG 4 cut(s) 46, 129, 355, 507
FalI AAGNNNNNCTT 2 cut(s) 185, 217
FaqI GGGAC 2 cut(s) 475, 527
FatI CATG 4 cut(s) 42, 125, 351, 503
Fnu4HI GCNGC 3 cut(s) 99, 236, 381
Fsp4HI GCNGC 3 cut(s) 99, 236, 381
GluI GCNGC 3 cut(s) 99, 236, 381
GsaI CCCAGC 1 cut(s) 265
HaeIII GGCC 2 cut(s) 171, 280
HapII CCGG 3 cut(s) 249, 366, 510
Hin1II CATG 4 cut(s) 46, 129, 355, 507
HincII GTYRAC 1 cut(s) 612
HindII GTYRAC 1 cut(s) 612
HindIII AAGCTT 1 cut(s) 430
HinfI GANTC 3 cut(s) 211, 413, 463
HpaII CCGG 3 cut(s) 249, 366, 510
HphI GGTGA 4 cut(s) 238, 381, 427, 676
Hpy166II GTNNAC 3 cut(s) 162, 349, 612
Hpy188I TCNGA 4 cut(s) 133, 210, 333, 678
Hpy188III TCNNGA 2 cut(s) 145, 621
Hpy8I GTNNAC 3 cut(s) 162, 349, 612
HpyAV CCTTC 2 cut(s) 334, 625
HpyCH4III ACNGT 2 cut(s) 113, 609
HpyCH4V TGCA 7 cut(s) 98, 137, 176, 380, 498, 594, 715
HpyF10VI GCNNNNNNNGC 4 cut(s) 241, 266, 504, 600
HpyF3I CTNAG 4 cut(s) 130, 305, 599, 619
Hsp92II CATG 4 cut(s) 46, 129, 355, 507
Kzo9I GATC 5 cut(s) 39, 121, 333, 386, 581
LmnI GCTCC 1 cut(s) 512
LpnPI CCDG 9 cut(s) 158, 262, 275, 327, 379, 523, 606, 640, 667
Lsp1109I GCAGC 2 cut(s) 110, 392
LweI GCATC 4 cut(s) 85, 124, 247, 318
MaeIII GTNAC 1 cut(s) 46
MalI GATC 5 cut(s) 41, 123, 335, 388, 583
MboI GATC 5 cut(s) 39, 121, 333, 386, 581
MflI RGATCY 1 cut(s) 581
MluCI AATT 4 cut(s) 449, 472, 488, 556
MlyI GAGTC 3 cut(s) 205, 407, 457
MmeI TCCRAC 1 cut(s) 70
MnlI CCTC 2 cut(s) 539, 655
MroXI GAANNNNTTC 1 cut(s) 488
MseI TTAA 3 cut(s) 204, 471, 648
MslI CAYNNNNRTG 1 cut(s) 41
MspA1I CMGCKG 1 cut(s) 101
MspI CCGG 3 cut(s) 249, 366, 510
MspR9I CCNGG 2 cut(s) 511, 655
MvaI CCWGG 1 cut(s) 655
MwoI GCNNNNNNNGC 4 cut(s) 241, 266, 504, 600
NciI CCSGG 1 cut(s) 511
NdeII GATC 5 cut(s) 39, 121, 333, 386, 581
NlaIII CATG 4 cut(s) 46, 129, 355, 507
NmuCI GTSAC 1 cut(s) 46
NspI RCATGY 1 cut(s) 507
PaeI GCATGC 1 cut(s) 507
PceI AGGCCT 1 cut(s) 280
PdmI GAANNNNTTC 1 cut(s) 488
PkrI GCNGC 3 cut(s) 100, 237, 382
PleI GAGTC 3 cut(s) 205, 407, 457
PpsI GAGTC 3 cut(s) 205, 407, 457
Psp6I CCWGG 1 cut(s) 653
PspFI CCCAGC 1 cut(s) 261
PspGI CCWGG 1 cut(s) 653
PstI CTGCAG 1 cut(s) 717
PsuI RGATCY 1 cut(s) 581
PvuII CAGCTG 1 cut(s) 101
RsaI GTAC 3 cut(s) 350, 444, 683
RsaNI GTAC 3 cut(s) 349, 443, 682
RseI CAYNNNNRTG 1 cut(s) 41
SaqAI TTAA 3 cut(s) 204, 471, 648
SatI GCNGC 3 cut(s) 99, 236, 381
Sau3AI GATC 5 cut(s) 39, 121, 333, 386, 581
ScaI AGTACT 1 cut(s) 683
SchI GAGTC 3 cut(s) 205, 407, 457
ScrFI CCNGG 2 cut(s) 511, 655
SetI ASST 9 cut(s) 103, 267, 326, 361, 434, 440, 600, 617, 666
SfaNI GCATC 4 cut(s) 85, 124, 247, 318
SfcI CTRYAG 1 cut(s) 713
SmiMI CAYNNNNRTG 1 cut(s) 41
SphI GCATGC 1 cut(s) 507
Sse9I AATT 4 cut(s) 449, 472, 488, 556
SseBI AGGCCT 1 cut(s) 280
SsiI CCGC 1 cut(s) 235
SspI AATATT 1 cut(s) 399
StuI AGGCCT 1 cut(s) 280
StyD4I CCNGG 2 cut(s) 509, 653
StyI CCWWGG 1 cut(s) 438
TaaI ACNGT 2 cut(s) 113, 609
TasI AATT 4 cut(s) 449, 472, 488, 556
TatI WGTACW 2 cut(s) 348, 681
TauI GCSGC 1 cut(s) 238
Tru1I TTAA 3 cut(s) 204, 471, 648
Tru9I TTAA 3 cut(s) 204, 471, 648
TscAI CASTG 2 cut(s) 118, 580
TseFI GTSAC 1 cut(s) 46
TseI GCWGC 2 cut(s) 98, 380
Tsp45I GTSAC 1 cut(s) 46
TspDTI ATGAA 1 cut(s) 552
TspGWI ACGGA 2 cut(s) 213, 385
TspRI CASTG 2 cut(s) 118, 580
XapI RAATTY 3 cut(s) 449, 488, 556
XceI RCATGY 1 cut(s) 507
XcmI CCANNNNNNNNNTGG 1 cut(s) 152
XmnI GAANNNNTTC 1 cut(s) 488
ZrmI AGTACT 1 cut(s) 683
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.