Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr5

Orthogroup ID

OG0012332

Physical Location & Seq

Genomic Coordinates Reverse (-)

23991514 .. 23994051

Gene Structure 2538 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom05g21360.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2538 bp

ATGTTTTATAGGAGGAAGCTCTGCCCTGTAAGTATTTCTAGACTCACCAAAAACCCTACAGTCAGCTCTGTGCTTCATAGTTCCAATTCACCGTACCCCAATCTGTGTGCAAATGAACTCCATAGTTATGGAGGAAGTTTCAGGTTATGGAGCTCTATAACGGGTTTCAATTCGATTTCTCATAGATTATCTGTACCGGCAAAACCCAATTTTGTGGGGAGCTCTGGTTTTTCAGGTACGAATTTGGTTGAAAAGCCGGTTTCTTTAGGCCCTGGTTTGATCAGAAAGTACTGTTCTGGGAAGAAGAGTGATGAGTGGATTGACGAGATTGACTATTTGGATGAATCAGGGAGTGTTATTTACACTGGGAAAGGTATAAGATCGGTTGAGCCAGGGCTCGATGACCATGTAATGGTAGGCGGGTTGAAAAAGCCAATGTTGAATGCCTCTGCCATTGCGAAAATTGTTGAGGTTGTAAAGAGGTGGAAATGGGGGCCTGAATTGGAGACCCAATTGGACAAACTCCAATTTGTACCAAACATGACTCACATTACTCAAGCGTTGAAGGTTATTAAAGATGGTGATGGGGCTTTGAGTTTGTTTCGGTGGGCTAAGAGGCAGTCATGGTATTTGCCGAGTGATGAGTGTTATGTGGTGTTGTTTGATGGCCTAAACCAAAGCAAGGACTTTGATGGAATCCAATCATTGTTTGATGAAATGGTGCAGGATTCTGGTAGTAGTGGGGTTTTGTCATCGGGTGCATATAACCGTGTCATTCAGTTCTTGGCTAAAGCAGATAAAATGGAGGTGGCATTTTGTTGCTTTAAGAAGATTCAAGATTCAGGTTTCGAAGTTGATACTCAGACGTATAATTCGCTTATATTGCTGTTTTTGAATAAGGGTTTACCTTATAAGGCATTTGAGATATATGAGAGCATGCAAGCTGCAAGCTGCTCATTAGATGGATCAACCTATGAGTTGATGGTACCAAACTTGGCAAAGTCAGGCCGCCTTGATGCTGCATTTAAGCTCTTCCAAGAAATGAAAGGGAGGAACTTTAAACCCAGCTTTAATGTCTTTGCATCGCTTGTTGATTCAATGGGAAAAGCTGGGCGGTTGGACACGTCGATGAAGTTATACATGGAAATGCAGGGCTATGGTTTCAGGCCATCTGCTCCCATGTATGTTTCTATGATTGAGTCATATGTAAGGGCTGGGAAATTAGATGCTGCCCTTAGACTTTGGGATGAGATGAAGAAAGCGGGCTTTAGGCCTAACTTTGGGTTATACACAATGATTGTTGAGTCCCATGCAAAATCAGGGAAACTTGATATTGCAATGTCTACCTTTACCGATATGGAGAAGGCTGGATTTCTACCCACTCCTTCGACATATTCATGTCTATTGGAAATGCATGCTGCCTCTGGACAAGTAGATTCTGCTATGAAGCTGTATAACTCAATGACCAATGCAGGTTTAAGACCAGGTCTAAGTACTTACACATCCCTTTTGATGCTTTTGGCTAATAAGAAGCTTGTGGACGTGGCTGCCAAGATTTTGCTTGAGATGAAGACGATGGGTTATTCTGTTGATGTGAGCGCTAGCGATGTTTTGATGGTGTATATCAAGGAGGGTTCTGTTGATGTAGCCTTGCGGTGGTTGCGCTTCATGGGCTCATCGGGGATTAGAACAAATAATTTTATAATCAGACAGTTGTTCGAGTCATGTATGAAGAGTGGTTTGTATGAGTCAGCAAAGCCTCTCTTAGAAACGTATGTGAACTCTGCTGCAAAAGTCGATCTCATACTTTACACATCCATTCTAGCGTATCTCGTAAGATGCCAGGAAGAAGAGCATGAGAGGCATTTGATGTCAATACTTGGTGCCACAAGACACGAGGCACACGCTTTTATGTGTGGACTCTTCACAGGCCCAGAACAGAGGAAACAACCAGTGTTATCTTTTGTGAGGGAATTTTTCCAAGGTATTGATTATGAGTTGGAAGAGGGACCTGCTAAGTACTTTGTTAATGTTCTTCTCAACTACCTTGTTCTTATGGGGCAGATAAATCGAGCCCGGTGTGTTTGGAAAGTTGCCTACGAGAATAAGCTTTTCCCAAAGGCAATAGTATTTGATCAGCTAATTGCTTGGTCCCTGGACGTCAGGAACTTATCAGTTGGAGCTGCTCTGATAGCAGTTGTGCATACTCTCCATAGGTTCAGAAAACGAATGTTATATTATGGTGTCATCCCAAGACGCATTAAATTGGTCACAGGACCGACTTTGAAGATAGTGGTTGCACAGATATTGAACTCGGTGGAATCTCCATTCGAGGTTAGCAAAGTGGTGCTGAGGGCACCGGGAGATGCTGTCATGGAGTGGTTTAAGAAACCCATTGTTCAACAGTTTCTTCTGAATGAGATTCCGTCAAGGTCCGATATACTCATGCACAAGATGAACACTCTTTTTCCTAGTTCTGCACCTGAATTAAGATCTCTGGCCCCTCCCAAAATACTCATGCCAAGGAAAGCAATGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

846

Amino Acids

94.68

Weight (kDa)

9.1

Isoelectric Point (pI)

36.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	253 - 294	1.7e-07	PPR repeat family
TPR_24	PF23276	253 - 382	1.8e-06	Fungal tetratrico peptide repeats
PPR_long	PF17177	256 - 407	3.2e-09	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	286 - 334	1.3e-06	PPR repeat family
PPR_3	PF13812	379 - 434	1.3e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	394 - 426	1.8e-07	PPR repeat family
PPR	PF01535	394 - 423	5.5e-07	PPR repeat
PPR_long	PF17177	408 - 494	1e-05	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	416 - 471	2e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	430 - 471	1.3e-06	PPR repeat family
TPR_24	PF23276	430 - 538	1.7e-09	Fungal tetratrico peptide repeats
PPR_2	PF13041	464 - 506	3.3e-08	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012332)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G79490
fragaria_vesca	FvH4_3g14450 FvH4_3g14450
malus_domestica	MD05G1236700.v1.1
prunus_persica	Prupe.4G129400_v2.0.a1
pyrus_communis	pycom05g21360
rosa_chinensis	RchiOBHm_Chr5g0023761
rosa_laevigata	RLG00000032735
rosa_multiflora	Rmu_sc0001372.1_g000009 Rmu_ssc0000027.1_g000014
rosa_roxburghii	Rroxscaffold_1G00055430
rosa_rugosa	Rorug05G0079500
rosa_samantha	Rh5AG169500 Rh5BG166900 Rh5CG182500 Rh5DG168800
rosa_wichuraiana	Rw0G001560 Rw0G005010 Rw5G015330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	912, 1703
AatII	GACGTC	1 cut(s)	2163
Acc36I	ACCTGC	2 cut(s)	1463, 2020
Acc65I	GGTACC	1 cut(s)	985
AccB1I	GGYRCC	3 cut(s)	985, 1883, 2356
AccB7I	CCANNNNNTGG	1 cut(s)	412
AccI	GTMKAC	1 cut(s)	1343
AciI	CCGC	5 cut(s)	420, 1009, 1114, 1262, 1654
AclWI	GGATC	1 cut(s)	973
AcsI	RAATTY	2 cut(s)	241, 1973
AcyI	GRCGYC	1 cut(s)	2160
AfaI	GTAC	8 cut(s)	95, 195, 238, 290, 534, 987, 1495, 2021
AfeI	AGCGCT	1 cut(s)	1600
AfiI	CCNNNNNNNGG	4 cut(s)	412, 682, 1280, 1656
AflIII	ACRYGT	1 cut(s)	1122
AjiI	CACGTC	2 cut(s)	1125, 1543
AjnI	CCWGG	5 cut(s)	271, 391, 1483, 1842, 2154
Alw21I	GWGCWC	2 cut(s)	155, 224
Alw26I	GTCTC	1 cut(s)	500
AlwI	GGATC	1 cut(s)	973
Aor51HI	AGCGCT	1 cut(s)	1600
AoxI	GGCC	8 cut(s)	268, 494, 667, 1006, 1166, 1271, 1930, 2499
ApeKI	GCWGC	8 cut(s)	944, 951, 1019, 1229, 1418, 1547, 1787, 2183
ApoI	RAATTY	2 cut(s)	241, 1973
ArsI	GACNNNNNNTTYG	2 cut(s)	856, 888
Asp718I	GGTACC	1 cut(s)	985
AspLEI	GCGC	2 cut(s)	1601, 1665
AspS9I	GGNCC	8 cut(s)	269, 494, 1931, 2009, 2151, 2276, 2433, 2500
AsuC2I	CCSGG	2 cut(s)	2077, 2361
AsuHPI	GGTGA	3 cut(s)	37, 81, 593
AsuII	TTCGAA	1 cut(s)	849
AsuNHI	GCTAGC	1 cut(s)	1601
AvaII	GGWCC	4 cut(s)	2009, 2151, 2276, 2433
BaeGI	GKGCMC	1 cut(s)	2359
BanI	GGYRCC	3 cut(s)	985, 1883, 2356
BanII	GRGCYC	5 cut(s)	155, 224, 399, 1676, 2077
BauI	CACGAG	1 cut(s)	1895
BbsI	GAAGAC	1 cut(s)	1577
Bbv12I	GWGCWC	2 cut(s)	155, 224
BbvCI	CCTCAGC	1 cut(s)	2351
BbvI	GCAGC	8 cut(s)	931, 938, 1006, 1216, 1405, 1534, 1774, 2170
BccI	CCATC	9 cut(s)	572, 578, 659, 686, 956, 976, 1177, 1570, 1609
BcgI	CGANNNNNNTGC	2 cut(s)	2051, 2085
BciT130I	CCWGG	5 cut(s)	273, 393, 1485, 1844, 2156
BclI	TGATCA	2 cut(s)	279, 2134
BcnI	CCSGG	2 cut(s)	2077, 2361
BcoDI	GTCTC	1 cut(s)	500
BfaI	CTAG	4 cut(s)	39, 1602, 1823, 2472
BfmI	CTRYAG	1 cut(s)	57
BfoI	RGCGCY	1 cut(s)	1602
BfuAI	ACCTGC	2 cut(s)	1463, 2020
BglII	AGATCT	1 cut(s)	2492
BisI	GCNGC	9 cut(s)	945, 952, 1009, 1020, 1230, 1419, 1548, 1788, 2184
BlsI	GCNGC	9 cut(s)	946, 953, 1010, 1021, 1231, 1420, 1549, 1789, 2185
BmcAI	AGTACT	3 cut(s)	290, 1495, 2021
Bme1390I	CCNGG	7 cut(s)	273, 393, 1485, 1844, 2077, 2156, 2361
Bme18I	GGWCC	4 cut(s)	2009, 2151, 2276, 2433
BmgBI	CACGTC	2 cut(s)	1125, 1543
BmgT120I	GGNCC	8 cut(s)	269, 494, 1931, 2009, 2151, 2276, 2433, 2500
BmiI	GGNNCC	7 cut(s)	495, 987, 1885, 2010, 2153, 2358, 2502
BmrFI	CCNGG	7 cut(s)	273, 393, 1485, 1844, 2077, 2156, 2361
BmrI	ACTGGG	1 cut(s)	375
BmsI	GCATC	6 cut(s)	1006, 1091, 1216, 1503, 1829, 2356
BmtI	GCTAGC	1 cut(s)	1605
BmuI	ACTGGG	1 cut(s)	375
BpiI	GAAGAC	1 cut(s)	1577
Bpu10I	CCTNAGC	1 cut(s)	2351
Bpu14I	TTCGAA	1 cut(s)	849
BpuEI	CTTGAG	2 cut(s)	540, 1583
BpuMI	CCSGG	2 cut(s)	2077, 2361
BsaHI	GRCGYC	1 cut(s)	2160
BsaI	GGTCTC	1 cut(s)	500
BsaJI	CCNNGG	5 cut(s)	271, 392, 1981, 2154, 2522
BsaXI	ACNNNNNCTCC	4 cut(s)	211, 241, 2355, 2385
Bsc4I	CCNNNNNNNGG	4 cut(s)	412, 682, 1280, 1656
Bse118I	RCCGGY	2 cut(s)	196, 256
Bse1I	ACTGG	2 cut(s)	370, 1952
Bse3DI	GCAATG	3 cut(s)	453, 1344, 2538
BseBI	CCWGG	5 cut(s)	273, 393, 1485, 1844, 2156
BseDI	CCNNGG	5 cut(s)	271, 392, 1981, 2154, 2522
BseGI	GGATG	5 cut(s)	346, 1252, 1502, 1814, 2247
BseLI	CCNNNNNNNGG	4 cut(s)	412, 682, 1280, 1656
BseMI	GCAATG	3 cut(s)	453, 1344, 2538
BseMII	CTCAG	2 cut(s)	875, 2342
BseNI	ACTGG	2 cut(s)	370, 1952
BseSI	GKGCMC	1 cut(s)	2359
BseXI	GCAGC	8 cut(s)	931, 938, 1006, 1216, 1405, 1534, 1774, 2170
BseYI	CCCAGC	3 cut(s)	1064, 1109, 1214
BsgI	GTGCAG	2 cut(s)	743, 2463
BshFI	GGCC	8 cut(s)	270, 496, 669, 1008, 1168, 1273, 1932, 2501
BshNI	GGYRCC	3 cut(s)	985, 1883, 2356
BsiHKAI	GWGCWC	2 cut(s)	155, 224
BsiSI	CCGG	4 cut(s)	197, 257, 2077, 2360
BslFI	GGGAC	3 cut(s)	1291, 2022, 2137
BslI	CCNNNNNNNGG	4 cut(s)	412, 682, 1280, 1656
BsmAI	GTCTC	1 cut(s)	500
BsmFI	GGGAC	3 cut(s)	1291, 2022, 2137
BsmI	GAATGC	1 cut(s)	448
BsnI	GGCC	8 cut(s)	270, 496, 669, 1008, 1168, 1273, 1932, 2501
Bso31I	GGTCTC	1 cut(s)	500
Bsp119I	TTCGAA	1 cut(s)	849
Bsp1286I	GDGCHC	6 cut(s)	155, 224, 399, 1676, 2077, 2359
Bsp143I	GATC	6 cut(s)	279, 380, 965, 1798, 2134, 2492
BspACI	CCGC	5 cut(s)	420, 1009, 1114, 1262, 1654
BspANI	GGCC	8 cut(s)	270, 496, 669, 1008, 1168, 1273, 1932, 2501
BspCNI	CTCAG	2 cut(s)	874, 2343
BspLI	GGNNCC	7 cut(s)	495, 987, 1885, 2010, 2153, 2358, 2502
BspMI	ACCTGC	2 cut(s)	1463, 2020
BspOI	GCTAGC	1 cut(s)	1605
BspPI	GGATC	1 cut(s)	973
BspQI	GCTCTTC	2 cut(s)	1037, 1845
BspT104I	TTCGAA	1 cut(s)	849
BspT107I	GGYRCC	3 cut(s)	985, 1883, 2356
BspTNI	GGTCTC	1 cut(s)	500
BsrDI	GCAATG	3 cut(s)	453, 1344, 2538
BsrFI	RCCGGY	2 cut(s)	196, 256
BsrI	ACTGG	2 cut(s)	370, 1952
BssAI	RCCGGY	2 cut(s)	196, 256
BssECI	CCNNGG	5 cut(s)	271, 392, 1981, 2154, 2522
BssMI	GATC	6 cut(s)	279, 380, 965, 1798, 2134, 2492
BssNI	GRCGYC	1 cut(s)	2160
BssSI	CACGAG	1 cut(s)	1895
BssT1I	CCWWGG	2 cut(s)	1981, 2522
Bst2BI	CACGAG	1 cut(s)	1895
Bst2UI	CCWGG	5 cut(s)	273, 393, 1485, 1844, 2156
Bst4CI	ACNGT	6 cut(s)	61, 93, 293, 770, 1713, 2406
Bst6I	CTCTTC	6 cut(s)	299, 1037, 1727, 1845, 1928, 1998
BstACI	GRCGYC	1 cut(s)	2160
BstBI	TTCGAA	1 cut(s)	849
BstC8I	GCNNGC	6 cut(s)	938, 942, 949, 1264, 1416, 1603
BstDEI	CTNAG	7 cut(s)	612, 861, 1235, 1490, 1765, 2016, 2351
BstF5I	GGATG	5 cut(s)	346, 1252, 1502, 1814, 2247
BstH2I	RGCGCY	1 cut(s)	1602
BstHHI	GCGC	2 cut(s)	1601, 1665
BstKTI	GATC	6 cut(s)	282, 383, 968, 1801, 2137, 2495
BstMAI	GTCTC	1 cut(s)	500
BstMBI	GATC	6 cut(s)	279, 380, 965, 1798, 2134, 2492
BstMWI	GCNNNNNNNGC	5 cut(s)	883, 1660, 1671, 1861, 2192
BstNI	CCWGG	5 cut(s)	273, 393, 1485, 1844, 2156
BstNSI	RCATGY	2 cut(s)	940, 1418
BstSCI	CCNGG	7 cut(s)	271, 391, 1483, 1842, 2075, 2154, 2359
BstSFI	CTRYAG	1 cut(s)	57
BstSLI	GKGCMC	1 cut(s)	2359
BstV1I	GCAGC	8 cut(s)	931, 938, 1006, 1216, 1405, 1534, 1774, 2170
BstV2I	GAAGAC	1 cut(s)	1577
BstX2I	RGATCY	1 cut(s)	2492
BstXI	CCANNNNNNTGG	2 cut(s)	128, 214
BstYI	RGATCY	1 cut(s)	2492
BsuRI	GGCC	8 cut(s)	270, 496, 669, 1008, 1168, 1273, 1932, 2501
BtgZI	GCGATG	2 cut(s)	1068, 1620
BtrI	CACGTC	2 cut(s)	1125, 1543
BtsCI	GGATG	5 cut(s)	346, 1252, 1502, 1814, 2247
BtsIMutI	CAGTG	2 cut(s)	363, 1959
BveI	ACCTGC	2 cut(s)	1463, 2020
Cac8I	GCNNGC	6 cut(s)	938, 942, 949, 1264, 1416, 1603
CfoI	GCGC	2 cut(s)	1601, 1665
Cfr10I	RCCGGY	2 cut(s)	196, 256
Cfr13I	GGNCC	8 cut(s)	269, 494, 1931, 2009, 2151, 2276, 2433, 2500
CseI	GACGC	1 cut(s)	2265
CsiI	ACCWGGT	1 cut(s)	1483
Csp6I	GTAC	8 cut(s)	94, 194, 237, 289, 533, 986, 1494, 2020
CviQI	GTAC	8 cut(s)	94, 194, 237, 289, 533, 986, 1494, 2020
DdeI	CTNAG	7 cut(s)	612, 861, 1235, 1490, 1765, 2016, 2351
DpnI	GATC	6 cut(s)	281, 382, 967, 1800, 2136, 2494
DpnII	GATC	6 cut(s)	279, 380, 965, 1798, 2134, 2492
DraI	TTTAAA	1 cut(s)	1060
Eam1104I	CTCTTC	6 cut(s)	299, 1037, 1727, 1845, 1928, 1998
EarI	CTCTTC	6 cut(s)	299, 1037, 1727, 1845, 1928, 1998
Ecl136II	GAGCTC	2 cut(s)	153, 222
Eco130I	CCWWGG	2 cut(s)	1981, 2522
Eco147I	AGGCCT	1 cut(s)	1273
Eco24I	GRGCYC	5 cut(s)	155, 224, 399, 1676, 2077
Eco31I	GGTCTC	1 cut(s)	500
Eco47I	GGWCC	4 cut(s)	2009, 2151, 2276, 2433
Eco47III	AGCGCT	1 cut(s)	1600
Eco53kI	GAGCTC	2 cut(s)	153, 222
EcoICRI	GAGCTC	2 cut(s)	153, 222
EcoO109I	RGGNCCY	3 cut(s)	269, 494, 2009
EcoRII	CCWGG	5 cut(s)	271, 391, 1483, 1842, 2154
EcoT14I	CCWWGG	2 cut(s)	1981, 2522
EcoT22I	ATGCAT	1 cut(s)	1416
EcoT38I	GRGCYC	5 cut(s)	155, 224, 399, 1676, 2077
ErhI	CCWWGG	2 cut(s)	1981, 2522
FalI	AAGNNNNNCTT	2 cut(s)	1748, 1780
FaqI	GGGAC	3 cut(s)	1291, 2022, 2137
FauI	CCCGC	2 cut(s)	413, 1255
FauNDI	CATATG	1 cut(s)	1204
FbaI	TGATCA	2 cut(s)	279, 2134
FblI	GTMKAC	1 cut(s)	1343
Fnu4HI	GCNGC	9 cut(s)	945, 952, 1009, 1020, 1230, 1419, 1548, 1788, 2184
FokI	GGATG	5 cut(s)	353, 1259, 1489, 1801, 2234
FriOI	GRGCYC	5 cut(s)	155, 224, 399, 1676, 2077
Fsp4HI	GCNGC	9 cut(s)	945, 952, 1009, 1020, 1230, 1419, 1548, 1788, 2184
FspBI	CTAG	4 cut(s)	39, 1602, 1823, 2472
GlaI	GCGC	2 cut(s)	1600, 1664
GluI	GCNGC	9 cut(s)	945, 952, 1009, 1020, 1230, 1419, 1548, 1788, 2184
GsaI	CCCAGC	3 cut(s)	1068, 1113, 1218
HaeII	RGCGCY	1 cut(s)	1602
HaeIII	GGCC	8 cut(s)	270, 496, 669, 1008, 1168, 1273, 1932, 2501
HapII	CCGG	4 cut(s)	197, 257, 2077, 2360
HgaI	GACGC	1 cut(s)	2265
HhaI	GCGC	2 cut(s)	1601, 1665
Hin1I	GRCGYC	1 cut(s)	2160
Hin6I	GCGC	2 cut(s)	1599, 1663
HinP1I	GCGC	2 cut(s)	1599, 1663
HindIII	AAGCTT	2 cut(s)	1532, 2108
HpaII	CCGG	4 cut(s)	197, 257, 2077, 2360
HphI	GGTGA	3 cut(s)	37, 81, 593
Hpy166II	GTNNAC	5 cut(s)	905, 1344, 1540, 1780, 1919
Hpy188I	TCNGA	7 cut(s)	284, 864, 1709, 2190, 2222, 2415, 2437
Hpy188III	TCNNGA	4 cut(s)	39, 836, 1425, 2164
Hpy8I	GTNNAC	5 cut(s)	905, 1344, 1540, 1780, 1919
Hpy99I	CGWCG	1 cut(s)	1129
HpyAV	CCTTC	3 cut(s)	559, 1357, 1395
HpyCH4III	ACNGT	6 cut(s)	61, 93, 293, 770, 1713, 2406
HpyCH4IV	ACGT	5 cut(s)	866, 1124, 1542, 1772, 2160
HpyF10VI	GCNNNNNNNGC	5 cut(s)	883, 1660, 1671, 1861, 2192
HpyF3I	CTNAG	7 cut(s)	612, 861, 1235, 1490, 1765, 2016, 2351
HpySE526I	ACGT	5 cut(s)	866, 1124, 1542, 1772, 2160
Hsp92I	GRCGYC	1 cut(s)	2160
HspAI	GCGC	2 cut(s)	1599, 1663
KpnI	GGTACC	1 cut(s)	989
Ksp22I	TGATCA	2 cut(s)	279, 2134
Kzo9I	GATC	6 cut(s)	279, 380, 965, 1798, 2134, 2492
LguI	GCTCTTC	2 cut(s)	1037, 1845
LmnI	GCTCC	4 cut(s)	150, 219, 1180, 2180
Lsp1109I	GCAGC	8 cut(s)	931, 938, 1006, 1216, 1405, 1534, 1774, 2170
LweI	GCATC	6 cut(s)	1006, 1091, 1216, 1503, 1829, 2356
MabI	ACCWGGT	1 cut(s)	1483
MaeI	CTAG	4 cut(s)	39, 1602, 1823, 2472
MaeII	ACGT	5 cut(s)	866, 1124, 1542, 1772, 2160
MaeIII	GTNAC	1 cut(s)	2269
MalI	GATC	6 cut(s)	281, 382, 967, 1800, 2136, 2494
MboI	GATC	6 cut(s)	279, 380, 965, 1798, 2134, 2492
MfeI	CAATTG	1 cut(s)	512
MflI	RGATCY	1 cut(s)	2492
MhlI	GDGCHC	6 cut(s)	155, 224, 399, 1676, 2077, 2359
MlyI	GAGTC	7 cut(s)	36, 538, 1208, 1313, 1730, 1757, 1914
MmeI	TCCRAC	3 cut(s)	1098, 1980, 2158
Mph1103I	ATGCAT	1 cut(s)	1416
MslI	CAYNNNNRTG	4 cut(s)	126, 1127, 1145, 1396
MspI	CCGG	4 cut(s)	197, 257, 2077, 2360
MspR9I	CCNGG	7 cut(s)	273, 393, 1485, 1844, 2077, 2156, 2361
MunI	CAATTG	1 cut(s)	512
Mva1269I	GAATGC	1 cut(s)	448
MvaI	CCWGG	5 cut(s)	273, 393, 1485, 1844, 2156
MwoI	GCNNNNNNNGC	5 cut(s)	883, 1660, 1671, 1861, 2192
NciI	CCSGG	2 cut(s)	2077, 2361
NdeI	CATATG	1 cut(s)	1204
NdeII	GATC	6 cut(s)	279, 380, 965, 1798, 2134, 2492
NheI	GCTAGC	1 cut(s)	1601
NlaIV	GGNNCC	7 cut(s)	495, 987, 1885, 2010, 2153, 2358, 2502
NmeAIII	GCCGAG	1 cut(s)	660
NmuCI	GTSAC	1 cut(s)	2269
NsiI	ATGCAT	1 cut(s)	1416
NspI	RCATGY	2 cut(s)	940, 1418
NspV	TTCGAA	1 cut(s)	849
PaeI	GCATGC	2 cut(s)	940, 1418
PceI	AGGCCT	1 cut(s)	1273
PciSI	GCTCTTC	2 cut(s)	1037, 1845
PctI	GAATGC	1 cut(s)	448
PfeI	GAWTC	9 cut(s)	344, 696, 728, 832, 839, 1094, 1436, 2321, 2422
PflFI	GACNNNGTC	1 cut(s)	1485
PflMI	CCANNNNNTGG	1 cut(s)	412
PkrI	GCNGC	9 cut(s)	946, 953, 1010, 1021, 1231, 1420, 1549, 1789, 2185
PleI	GAGTC	7 cut(s)	36, 538, 1207, 1312, 1729, 1756, 1914
PpsI	GAGTC	7 cut(s)	36, 538, 1207, 1312, 1729, 1756, 1914
PpuMI	RGGWCCY	1 cut(s)	2009
PsiI	TTATAA	2 cut(s)	912, 1703
Psp124BI	GAGCTC	2 cut(s)	155, 224
Psp5II	RGGWCCY	1 cut(s)	2009
Psp6I	CCWGG	5 cut(s)	271, 391, 1483, 1842, 2154
PspFI	CCCAGC	3 cut(s)	1064, 1109, 1214
PspGI	CCWGG	5 cut(s)	271, 391, 1483, 1842, 2154
PspN4I	GGNNCC	7 cut(s)	495, 987, 1885, 2010, 2153, 2358, 2502
PspPI	GGNCC	8 cut(s)	269, 494, 1931, 2009, 2151, 2276, 2433, 2500
PspPPI	RGGWCCY	1 cut(s)	2009
PsuI	RGATCY	1 cut(s)	2492
PsyI	GACNNNGTC	1 cut(s)	1485
RsaI	GTAC	8 cut(s)	95, 195, 238, 290, 534, 987, 1495, 2021
RsaNI	GTAC	8 cut(s)	94, 194, 237, 289, 533, 986, 1494, 2020
RseI	CAYNNNNRTG	4 cut(s)	126, 1127, 1145, 1396
SacI	GAGCTC	2 cut(s)	155, 224
SapI	GCTCTTC	2 cut(s)	1037, 1845
SatI	GCNGC	9 cut(s)	945, 952, 1009, 1020, 1230, 1419, 1548, 1788, 2184
Sau3AI	GATC	6 cut(s)	279, 380, 965, 1798, 2134, 2492
Sau96I	GGNCC	8 cut(s)	269, 494, 1931, 2009, 2151, 2276, 2433, 2500
ScaI	AGTACT	3 cut(s)	290, 1495, 2021
SchI	GAGTC	7 cut(s)	36, 538, 1208, 1313, 1730, 1757, 1914
ScrFI	CCNGG	7 cut(s)	273, 393, 1485, 1844, 2077, 2156, 2361
SduI	GDGCHC	6 cut(s)	155, 224, 399, 1676, 2077, 2359
SexAI	ACCWGGT	1 cut(s)	1483
SfaNI	GCATC	6 cut(s)	1006, 1091, 1216, 1503, 1829, 2356
SfcI	CTRYAG	1 cut(s)	57
SfuI	TTCGAA	1 cut(s)	849
SinI	GGWCC	4 cut(s)	2009, 2151, 2276, 2433
SmiMI	CAYNNNNRTG	4 cut(s)	126, 1127, 1145, 1396
SmlI	CTYRAG	2 cut(s)	555, 1562
SmoI	CTYRAG	2 cut(s)	555, 1562
SphI	GCATGC	2 cut(s)	940, 1418
SseBI	AGGCCT	1 cut(s)	1273
SsiI	CCGC	5 cut(s)	420, 1009, 1114, 1262, 1654
SspMI	CTAG	4 cut(s)	39, 1602, 1823, 2472
SstI	GAGCTC	2 cut(s)	155, 224
StuI	AGGCCT	1 cut(s)	1273
StyD4I	CCNGG	7 cut(s)	271, 391, 1483, 1842, 2075, 2154, 2359
StyI	CCWWGG	2 cut(s)	1981, 2522
TaaI	ACNGT	6 cut(s)	61, 93, 293, 770, 1713, 2406
TaiI	ACGT	5 cut(s)	869, 1127, 1545, 1775, 2163
TaqI	TCGA	9 cut(s)	173, 399, 849, 1127, 1388, 1719, 1797, 2071, 2331
TaqII	GACCGA	1 cut(s)	2293
TatI	WGTACW	3 cut(s)	288, 1493, 2019
TauI	GCSGC	1 cut(s)	1011
TfiI	GAWTC	9 cut(s)	344, 696, 728, 832, 839, 1094, 1436, 2321, 2422
TscAI	CASTG	2 cut(s)	370, 1959
TseFI	GTSAC	1 cut(s)	2269
TseI	GCWGC	8 cut(s)	944, 951, 1019, 1229, 1418, 1547, 1787, 2183
Tsp45I	GTSAC	1 cut(s)	2269
TspGWI	ACGGA	1 cut(s)	2415
TspRI	CASTG	2 cut(s)	370, 1959
Tth111I	GACNNNGTC	1 cut(s)	1485
Van91I	CCANNNNNTGG	1 cut(s)	412
VpaK11BI	GGWCC	4 cut(s)	2009, 2151, 2276, 2433
XapI	RAATTY	2 cut(s)	241, 1973
XbaI	TCTAGA	1 cut(s)	38
XceI	RCATGY	2 cut(s)	940, 1418
XmiI	GTMKAC	1 cut(s)	1343
XspI	CTAG	4 cut(s)	39, 1602, 1823, 2472
ZraI	GACGTC	1 cut(s)	2161
ZrmI	AGTACT	3 cut(s)	290, 1495, 2021
Zsp2I	ATGCAT	1 cut(s)	1416

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.