Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr5

Orthogroup ID

OG0008771

Physical Location & Seq

Genomic Coordinates Reverse (-)

24457162 .. 24460060

Gene Structure 2899 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1530 bp

ATGGAGGCTTACGGCGGATACTTGGTGGACGAGAAGGCGGTGAGGGTAGAGTCCATCTTCGTCGAGTTCCTCAAGAACTTCAGGATCCCCGGAGGAGGGGGAGAGGCGTATTACGAGGGGGAGATCGAAGCCATGGCCGCCAACGAGTCCAGCACCATGTTCATCGATTTCTCCCACGTCATGGTTTTCAACAATCTCCTCCAGAAGGCCATTTCCGACGAGTTTCTCAGGTTTGAGCCCTATTTGAAGAACGCTTGCAAGACATTCGTGACGCAGCTCCGCCCAAACTTCATCTCCGACGATACGAACAAGGACATCAATGTCGCCTTCTTCAACCTCCCCGTCTCCAAGAGGTTGAGGGAATTGACCACAGCGGAAATTGGGAAATTGGTATCGGTGAAAGGAGTGGTGACCCGGACCAGTGAGGTTCGACCGGAGCTACTACACGGGACATTCAAGTGCTTGGAGTGCGGAGGCGTCATCAAGAATGTCGAACAACAATTCAAGTACACTGAGGAGACGTCCAAAGAGATACCTGCGGGCTCCCTTCCCAGATCACTGGATGTTATTATCCGCCACGAGATTGTTGAGAAGGCCAGGGCCGGTGACACGGTCATTTTCACGGGCACGGTGGTTGTCATACCGGACATAATGGCATTGGCCTCCCCTGGAGACAGAGCAGAGTGTTCTCGACAAGCATCTCAGCGCAATGGTTCTATGGCTGGACATGAAGGAGTGAGGGGTCTTAAAGCATTGGGAGTGAGAGACCTCTCCTATCGCCTTGCCTTTATTGCCAATTCAGTTCAGATTTCTGATGGTAGACAGGATACTGATGTCCGGAACAGAAAGAAGGATGCTGACGATGATGACAACCAGCAGTTCACTGAAGAAGAGAAAGACGAAGTTCAAAGAATGAGGAATACCCCCGATTTCTTCAATAAGCTTGTTGATAGCGTTGCCCCAACTGTATTTGGCCATCAAGATATCAAGCGTGCAATTCTGCTTATGCTCCTGGGTGGTGTCCACAAGTTTACTCATGAAGGCATCAACTTGAGAGGAGACATCAATGTTTGTATAGTTGGAGACCCCAGTTGCGCAAAATCCCAGTTCCTAAAGTATACGGCAGGAATAGTTCCAAGATCCGTTCACACTTCTGGGAAATCCTCCTCTGCTGCTGGTTTAACAGCAACTGTTGCCAAAGAACCAGAAACAGGAGAATTTTGTATTGAGGCAGGTGCGTTAATGCTTGCTGACAATGGTATTTGTTGCATTGATGAATTTGACAAAATGGACATTAGGGATCAGGTTGCGATTCATGAGGCAATGGAACAACAGACAATAAGCATTACAAAAGCAGGAATACAGGCAACACTGAATGCTCGAACATCAATTCTTGCTGCTGCTAACCCCACAGGCGGCCGTTATGATAAATCTAAACCACTCAAGTATAATGTGGCTCTTCCTCCACCTATCCTCTCAAGGTTTGATCTAGTATATGTTATGATTGATGACGCAGATCGATCAAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000347 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006268 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0016043 GO:0032392 GO:0032508 GO:0032991 GO:0034641 GO:0034645 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0051276 GO:0071103 GO:0071704 GO:0071840 GO:0090304 GO:1901360 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko03030 ko04110 ko04111 ko04113 map03030 map04110 map04111 map04113

Pfam Domains

Protein Families

MCM MCM_N MCM_OB

Protein Analysis

510

Amino Acids

56.08

Weight (kDa)

5.49

Isoelectric Point (pI)

34.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MCM_N	PF14551	20 - 112	1.4e-15	MCM N-terminal domain
MCM_OB	PF17207	119 - 164	9e-11	MCM OB domain
MCM_OB	PF17207	171 - 214	2.5e-09	MCM OB domain
MCM	PF00493	299 - 508	5e-96	MCM P-loop domain
Mg_chelatase	PF01078	408 - 498	1.1e-06	Magnesium chelatase, subunit ChlI

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008771)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G44635 AT5G44635
fragaria_vesca	FvH4_3g13360 FvH4_3g13360 FvH4_3g13360 FvH4_3g13360
malus_domestica	MD05G1242400.v1.1 MD10G1224200.v1.1
prunus_persica	Prupe.4G121000_v2.0.a1 Prupe.4G121000_v2.0.a1 Prupe.4G121000_v2.0.a1
pyrus_communis	pycom05g21890 pycom10g18820
rosa_chinensis	RchiOBHm_Chr5g0022071
rosa_laevigata	RLG00000032641
rosa_multiflora	Rmu_sc0006966.1_g000002
rosa_roxburghii	Rroxscaffold_1G00056860
rosa_rugosa	Rorug05G0070900
rosa_samantha	Rh5AG158900 Rh5BG158200 Rh5CG172600 Rh5DG158400
rosa_wichuraiana	Rw5G014180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1223
AasI	GACNNNNNNGTC	1 cut(s)	320
AatII	GACGTC	1 cut(s)	524
Acc16I	TGCGCA	1 cut(s)	1096
Acc36I	ACCTGC	2 cut(s)	544, 1223
AccB7I	CCANNNNNTGG	1 cut(s)	181
AccI	GTMKAC	2 cut(s)	820, 1118
AccIII	TCCGGA	1 cut(s)	837
AciI	CCGC	9 cut(s)	15, 38, 138, 280, 374, 471, 539, 574, 1416
AclWI	GGATC	4 cut(s)	79, 92, 1134, 1308
AcoI	YGGCCR	3 cut(s)	135, 973, 1417
AcsI	RAATTY	2 cut(s)	1217, 1277
AcuI	CTGAAG	2 cut(s)	64, 906
AcyI	GRCGYC	2 cut(s)	477, 521
AfaI	GTAC	1 cut(s)	509
AfiI	CCNNNNNNNGG	6 cut(s)	95, 96, 181, 205, 1211, 1415
AgsI	TTSAA	7 cut(s)	190, 247, 334, 457, 505, 908, 937
AjiI	CACGTC	1 cut(s)	178
AjnI	CCWGG	3 cut(s)	596, 667, 1011
AluBI	AGCT	3 cut(s)	277, 439, 943
AluI	AGCT	3 cut(s)	277, 439, 943
Alw26I	GTCTC	6 cut(s)	349, 512, 666, 759, 1053, 1077
AlwI	GGATC	4 cut(s)	79, 92, 1134, 1308
AlwNI	CAGNNNCTG	1 cut(s)	1190
Aor13HI	TCCGGA	1 cut(s)	837
AoxI	GGCC	7 cut(s)	135, 207, 594, 600, 660, 973, 1417
ApeKI	GCWGC	4 cut(s)	274, 1172, 1397, 1400
ApoI	RAATTY	2 cut(s)	1217, 1277
AspLEI	GCGC	2 cut(s)	708, 1097
AspS9I	GGNCC	2 cut(s)	417, 600
AsuC2I	CCSGG	2 cut(s)	90, 415
AsuHPI	GGTGA	4 cut(s)	52, 409, 421, 617
AvaII	GGWCC	1 cut(s)	417
BaeGI	GKGCMC	1 cut(s)	629
BaeI	ACNNNNGTAYC	2 cut(s)	819, 852
BalI	TGGCCA	1 cut(s)	975
BamHI	GGATCC	1 cut(s)	84
BanII	GRGCYC	2 cut(s)	240, 545
BauI	CACGAG	1 cut(s)	578
BbvI	GCAGC	4 cut(s)	286, 1159, 1384, 1387
BccI	CCATC	3 cut(s)	62, 809, 984
BceAI	ACGGC	3 cut(s)	28, 1137, 1404
BcgI	CGANNNNNNTGC	2 cut(s)	247, 281
BciT130I	CCWGG	3 cut(s)	598, 669, 1013
BciVI	GTATCC	2 cut(s)	11, 820
BcnI	CCSGG	2 cut(s)	90, 415
BcoDI	GTCTC	6 cut(s)	349, 512, 666, 759, 1053, 1077
BfaI	CTAG	1 cut(s)	1490
BfuAI	ACCTGC	2 cut(s)	544, 1223
BfuI	GTATCC	2 cut(s)	11, 820
BisI	GCNGC	6 cut(s)	138, 275, 1173, 1398, 1401, 1417
BlsI	GCNGC	6 cut(s)	139, 276, 1174, 1399, 1402, 1418
Bme1390I	CCNGG	5 cut(s)	90, 415, 598, 669, 1013
Bme18I	GGWCC	1 cut(s)	417
BmgBI	CACGTC	1 cut(s)	178
BmgT120I	GGNCC	2 cut(s)	417, 600
BmiI	GGNNCC	2 cut(s)	86, 544
BmrFI	CCNGG	5 cut(s)	90, 415, 598, 669, 1013
BmrI	ACTGGG	2 cut(s)	1083, 1099
BmsI	GCATC	3 cut(s)	707, 844, 1053
BmuI	ACTGGG	2 cut(s)	1083, 1099
BpmI	CTGGAG	2 cut(s)	185, 690
BpuEI	CTTGAG	4 cut(s)	56, 1072, 1427, 1462
BpuMI	CCSGG	2 cut(s)	90, 415
Bsa29I	ATCGAT	2 cut(s)	165, 1519
BsaHI	GRCGYC	2 cut(s)	477, 521
BsaI	GGTCTC	2 cut(s)	759, 1077
BsaJI	CCNNGG	5 cut(s)	88, 132, 597, 667, 1012
BsaWI	WCCGGW	3 cut(s)	433, 643, 837
BsaXI	ACNNNNNCTCC	2 cut(s)	278, 308
Bsc4I	CCNNNNNNNGG	6 cut(s)	95, 96, 181, 205, 1211, 1415
Bse118I	RCCGGY	1 cut(s)	602
Bse1I	ACTGG	4 cut(s)	420, 564, 1089, 1105
Bse3DI	GCAATG	2 cut(s)	715, 1329
BseAI	TCCGGA	1 cut(s)	837
BseBI	CCWGG	3 cut(s)	598, 669, 1013
BseCI	ATCGAT	2 cut(s)	165, 1519
BseDI	CCNNGG	5 cut(s)	88, 132, 597, 667, 1012
BseGI	GGATG	2 cut(s)	568, 859
BseLI	CCNNNNNNNGG	6 cut(s)	95, 96, 181, 205, 1211, 1415
BseMI	GCAATG	2 cut(s)	715, 1329
BseMII	CTCAG	3 cut(s)	241, 504, 716
BseNI	ACTGG	4 cut(s)	420, 564, 1089, 1105
BseRI	GAGGAG	5 cut(s)	108, 188, 530, 1071, 1156
BseSI	GKGCMC	1 cut(s)	629
BseX3I	CGGCCG	1 cut(s)	1417
BseXI	GCAGC	4 cut(s)	286, 1159, 1384, 1387
Bsh1285I	CGRYCG	2 cut(s)	434, 1420
BshFI	GGCC	7 cut(s)	137, 209, 596, 602, 662, 975, 1419
BshVI	ATCGAT	2 cut(s)	165, 1519
BsiEI	CGRYCG	2 cut(s)	434, 1420
BsiSI	CCGG	6 cut(s)	90, 415, 434, 603, 644, 838
BslFI	GGGAC	1 cut(s)	463
BslI	CCNNNNNNNGG	6 cut(s)	95, 96, 181, 205, 1211, 1415
BsmAI	GTCTC	6 cut(s)	349, 512, 666, 759, 1053, 1077
BsmBI	CGTCTC	2 cut(s)	349, 512
BsmFI	GGGAC	1 cut(s)	463
BsmI	GAATGC	1 cut(s)	1381
BsnI	GGCC	7 cut(s)	137, 209, 596, 602, 662, 975, 1419
Bso31I	GGTCTC	2 cut(s)	759, 1077
Bsp1286I	GDGCHC	3 cut(s)	240, 545, 629
Bsp13I	TCCGGA	1 cut(s)	837
Bsp143I	GATC	8 cut(s)	84, 123, 554, 1139, 1300, 1486, 1516, 1520
Bsp19I	CCATGG	1 cut(s)	132
BspACI	CCGC	9 cut(s)	15, 38, 138, 280, 374, 471, 539, 574, 1416
BspANI	GGCC	7 cut(s)	137, 209, 596, 602, 662, 975, 1419
BspCNI	CTCAG	3 cut(s)	240, 505, 715
BspDI	ATCGAT	2 cut(s)	165, 1519
BspEI	TCCGGA	1 cut(s)	837
BspHI	TCATGA	2 cut(s)	1036, 1315
BspLI	GGNNCC	2 cut(s)	86, 544
BspMI	ACCTGC	2 cut(s)	544, 1223
BspPI	GGATC	4 cut(s)	79, 92, 1134, 1308
BspQI	GCTCTTC	1 cut(s)	1464
BspTNI	GGTCTC	2 cut(s)	759, 1077
BsrDI	GCAATG	2 cut(s)	715, 1329
BsrFI	RCCGGY	1 cut(s)	602
BsrI	ACTGG	4 cut(s)	420, 564, 1089, 1105
BssAI	RCCGGY	1 cut(s)	602
BssECI	CCNNGG	5 cut(s)	88, 132, 597, 667, 1012
BssMI	GATC	8 cut(s)	84, 123, 554, 1139, 1300, 1486, 1516, 1520
BssNAI	GTATAC	1 cut(s)	1119
BssNI	GRCGYC	2 cut(s)	477, 521
BssSI	CACGAG	1 cut(s)	578
BssT1I	CCWWGG	1 cut(s)	132
Bst1107I	GTATAC	1 cut(s)	1119
Bst2BI	CACGAG	1 cut(s)	578
Bst2UI	CCWGG	3 cut(s)	598, 669, 1013
Bst4CI	ACNGT	4 cut(s)	613, 631, 967, 1192
Bst6I	CTCTTC	2 cut(s)	885, 1464
BstACI	GRCGYC	2 cut(s)	477, 521
BstAPI	GCANNNNNTGC	1 cut(s)	1193
BstC8I	GCNNGC	4 cut(s)	256, 541, 993, 1248
BstDEI	CTNAG	3 cut(s)	227, 513, 702
BstDSI	CCRYGG	1 cut(s)	132
BstEII	GGTNACC	1 cut(s)	409
BstENI	CCTNNNNNAGG	1 cut(s)	203
BstF5I	GGATG	2 cut(s)	568, 859
BstHHI	GCGC	2 cut(s)	708, 1097
BstKTI	GATC	8 cut(s)	87, 126, 557, 1142, 1303, 1489, 1519, 1523
BstMAI	GTCTC	6 cut(s)	349, 512, 666, 759, 1053, 1077
BstMBI	GATC	8 cut(s)	84, 123, 554, 1139, 1300, 1486, 1516, 1520
BstMCI	CGRYCG	2 cut(s)	434, 1420
BstMWI	GCNNNNNNNGC	4 cut(s)	137, 468, 791, 1193
BstNI	CCWGG	3 cut(s)	598, 669, 1013
BstPI	GGTNACC	1 cut(s)	409
BstSCI	CCNGG	5 cut(s)	88, 413, 596, 667, 1011
BstSLI	GKGCMC	1 cut(s)	629
BstV1I	GCAGC	4 cut(s)	286, 1159, 1384, 1387
BstX2I	RGATCY	2 cut(s)	84, 1139
BstXI	CCANNNNNNTGG	1 cut(s)	559
BstYI	RGATCY	2 cut(s)	84, 1139
BstZ17I	GTATAC	1 cut(s)	1119
BstZI	CGGCCG	1 cut(s)	1417
Bsu15I	ATCGAT	2 cut(s)	165, 1519
BsuI	GTATCC	2 cut(s)	11, 820
BsuRI	GGCC	7 cut(s)	137, 209, 596, 602, 662, 975, 1419
BsuTUI	ATCGAT	2 cut(s)	165, 1519
BtgI	CCRYGG	1 cut(s)	132
BtrI	CACGTC	1 cut(s)	178
BtsCI	GGATG	2 cut(s)	568, 859
BtsIMutI	CAGTG	5 cut(s)	427, 510, 557, 882, 1370
BveI	ACCTGC	2 cut(s)	544, 1223
Cac8I	GCNNGC	4 cut(s)	256, 541, 993, 1248
CaiI	CAGNNNCTG	1 cut(s)	1190
CciI	TCATGA	2 cut(s)	1036, 1315
CfoI	GCGC	2 cut(s)	708, 1097
Cfr10I	RCCGGY	1 cut(s)	602
Cfr13I	GGNCC	2 cut(s)	417, 600
ClaI	ATCGAT	2 cut(s)	165, 1519
CseI	GACGC	3 cut(s)	280, 466, 1520
Csp6I	GTAC	1 cut(s)	508
CviAII	CATG	6 cut(s)	133, 157, 181, 728, 1037, 1316
CviQI	GTAC	1 cut(s)	508
DdeI	CTNAG	3 cut(s)	227, 513, 702
DpnI	GATC	8 cut(s)	86, 125, 556, 1141, 1302, 1488, 1518, 1522
DpnII	GATC	8 cut(s)	84, 123, 554, 1139, 1300, 1486, 1516, 1520
DrdI	GACNNNNNNGTC	1 cut(s)	320
DseDI	GACNNNNNNGTC	1 cut(s)	320
EaeI	YGGCCR	3 cut(s)	135, 973, 1417
EagI	CGGCCG	1 cut(s)	1417
Eam1104I	CTCTTC	2 cut(s)	885, 1464
EarI	CTCTTC	2 cut(s)	885, 1464
EciI	GGCGGA	3 cut(s)	30, 269, 563
EclXI	CGGCCG	1 cut(s)	1417
Eco130I	CCWWGG	1 cut(s)	132
Eco24I	GRGCYC	2 cut(s)	240, 545
Eco31I	GGTCTC	2 cut(s)	759, 1077
Eco32I	GATATC	1 cut(s)	985
Eco47I	GGWCC	1 cut(s)	417
Eco52I	CGGCCG	1 cut(s)	1417
Eco57I	CTGAAG	2 cut(s)	64, 906
Eco91I	GGTNACC	1 cut(s)	409
EcoNI	CCTNNNNNAGG	1 cut(s)	203
EcoO65I	GGTNACC	1 cut(s)	409
EcoRII	CCWGG	3 cut(s)	596, 667, 1011
EcoRV	GATATC	1 cut(s)	985
EcoT14I	CCWWGG	1 cut(s)	132
EcoT38I	GRGCYC	2 cut(s)	240, 545
ErhI	CCWWGG	1 cut(s)	132
Esp3I	CGTCTC	2 cut(s)	349, 512
FaeI	CATG	6 cut(s)	136, 160, 184, 731, 1040, 1319
FaqI	GGGAC	1 cut(s)	463
FatI	CATG	6 cut(s)	132, 156, 180, 727, 1036, 1315
FauI	CCCGC	1 cut(s)	532
FblI	GTMKAC	2 cut(s)	820, 1118
Fnu4HI	GCNGC	6 cut(s)	138, 275, 1173, 1398, 1401, 1417
FokI	GGATG	2 cut(s)	575, 866
FriOI	GRGCYC	2 cut(s)	240, 545
Fsp4HI	GCNGC	6 cut(s)	138, 275, 1173, 1398, 1401, 1417
FspBI	CTAG	1 cut(s)	1490
FspI	TGCGCA	1 cut(s)	1096
GlaI	GCGC	2 cut(s)	707, 1096
GluI	GCNGC	6 cut(s)	138, 275, 1173, 1398, 1401, 1417
GsuI	CTGGAG	2 cut(s)	185, 690
HaeIII	GGCC	7 cut(s)	137, 209, 596, 602, 662, 975, 1419
HapII	CCGG	6 cut(s)	90, 415, 434, 603, 644, 838
HgaI	GACGC	3 cut(s)	280, 466, 1520
HhaI	GCGC	2 cut(s)	708, 1097
Hin1I	GRCGYC	2 cut(s)	477, 521
Hin1II	CATG	6 cut(s)	136, 160, 184, 731, 1040, 1319
Hin6I	GCGC	2 cut(s)	706, 1095
HinP1I	GCGC	2 cut(s)	706, 1095
HindIII	AAGCTT	1 cut(s)	941
HinfI	GANTC	3 cut(s)	50, 146, 1312
HpaII	CCGG	6 cut(s)	90, 415, 434, 603, 644, 838
HphI	GGTGA	4 cut(s)	52, 409, 421, 617
Hpy166II	GTNNAC	8 cut(s)	28, 510, 821, 882, 1024, 1032, 1119, 1147
Hpy188I	TCNGA	4 cut(s)	217, 298, 807, 814
Hpy8I	GTNNAC	8 cut(s)	28, 510, 821, 882, 1024, 1032, 1119, 1147
Hpy99I	CGWCG	3 cut(s)	65, 221, 302
HpyAV	CCTTC	8 cut(s)	28, 199, 337, 557, 586, 725, 844, 1034
HpyCH4III	ACNGT	4 cut(s)	613, 631, 967, 1192
HpyCH4IV	ACGT	2 cut(s)	177, 521
HpyCH4V	TGCA	3 cut(s)	258, 995, 1269
HpyF10VI	GCNNNNNNNGC	4 cut(s)	137, 468, 791, 1193
HpyF3I	CTNAG	3 cut(s)	227, 513, 702
HpySE526I	ACGT	2 cut(s)	177, 521
Hsp92I	GRCGYC	2 cut(s)	477, 521
Hsp92II	CATG	6 cut(s)	136, 160, 184, 731, 1040, 1319
HspAI	GCGC	2 cut(s)	706, 1095
Kpn2I	TCCGGA	1 cut(s)	837
Kzo9I	GATC	8 cut(s)	84, 123, 554, 1139, 1300, 1486, 1516, 1520
LguI	GCTCTTC	1 cut(s)	1464
LmnI	GCTCC	4 cut(s)	282, 436, 548, 1014
Lsp1109I	GCAGC	4 cut(s)	286, 1159, 1384, 1387
LweI	GCATC	3 cut(s)	707, 844, 1053
MaeI	CTAG	1 cut(s)	1490
MaeII	ACGT	2 cut(s)	177, 521
MaeIII	GTNAC	3 cut(s)	268, 409, 605
MalI	GATC	8 cut(s)	86, 125, 556, 1141, 1302, 1488, 1518, 1522
MboI	GATC	8 cut(s)	84, 123, 554, 1139, 1300, 1486, 1516, 1520
MboII	GAAGA	7 cut(s)	49, 259, 322, 899, 902, 925, 1451
MflI	RGATCY	2 cut(s)	84, 1139
MhlI	GDGCHC	3 cut(s)	240, 545, 629
MlsI	TGGCCA	1 cut(s)	975
MluCI	AATT	9 cut(s)	362, 378, 386, 500, 796, 996, 1217, 1277, 1389
MluNI	TGGCCA	1 cut(s)	975
MlyI	GAGTC	2 cut(s)	59, 155
MmeI	TCCRAC	3 cut(s)	240, 321, 1060
Mox20I	TGGCCA	1 cut(s)	975
MroI	TCCGGA	1 cut(s)	837
MscI	TGGCCA	1 cut(s)	975
MseI	TTAA	3 cut(s)	747, 1181, 1241
MslI	CAYNNNNRTG	1 cut(s)	457
Msp20I	TGGCCA	1 cut(s)	975
MspA1I	CMGCKG	1 cut(s)	374
MspI	CCGG	6 cut(s)	90, 415, 434, 603, 644, 838
MspR9I	CCNGG	5 cut(s)	90, 415, 598, 669, 1013
Mva1269I	GAATGC	1 cut(s)	1381
MvaI	CCWGG	3 cut(s)	598, 669, 1013
MwoI	GCNNNNNNNGC	4 cut(s)	137, 468, 791, 1193
NciI	CCSGG	2 cut(s)	90, 415
NcoI	CCATGG	1 cut(s)	132
NdeII	GATC	8 cut(s)	84, 123, 554, 1139, 1300, 1486, 1516, 1520
NlaIII	CATG	6 cut(s)	136, 160, 184, 731, 1040, 1319
NlaIV	GGNNCC	2 cut(s)	86, 544
NmuCI	GTSAC	3 cut(s)	268, 409, 605
NsbI	TGCGCA	1 cut(s)	1096
PagI	TCATGA	2 cut(s)	1036, 1315
PaqCI	CACCTGC	1 cut(s)	1223
PciSI	GCTCTTC	1 cut(s)	1464
PctI	GAATGC	1 cut(s)	1381
PfeI	GAWTC	1 cut(s)	1312
PflFI	GACNNNGTC	1 cut(s)	611
PflMI	CCANNNNNTGG	1 cut(s)	181
PkrI	GCNGC	6 cut(s)	139, 276, 1174, 1399, 1402, 1418
PleI	GAGTC	2 cut(s)	58, 154
PpsI	GAGTC	2 cut(s)	58, 154
Psp6I	CCWGG	3 cut(s)	596, 667, 1011
PspEI	GGTNACC	1 cut(s)	409
PspGI	CCWGG	3 cut(s)	596, 667, 1011
PspN4I	GGNNCC	2 cut(s)	86, 544
PspPI	GGNCC	2 cut(s)	417, 600
PstNI	CAGNNNCTG	1 cut(s)	1190
PsuI	RGATCY	2 cut(s)	84, 1139
PsyI	GACNNNGTC	1 cut(s)	611
RsaI	GTAC	1 cut(s)	509
RsaNI	GTAC	1 cut(s)	508
RseI	CAYNNNNRTG	1 cut(s)	457
SapI	GCTCTTC	1 cut(s)	1464
SaqAI	TTAA	3 cut(s)	747, 1181, 1241
SatI	GCNGC	6 cut(s)	138, 275, 1173, 1398, 1401, 1417
Sau3AI	GATC	8 cut(s)	84, 123, 554, 1139, 1300, 1486, 1516, 1520
Sau96I	GGNCC	2 cut(s)	417, 600
SchI	GAGTC	2 cut(s)	59, 155
ScrFI	CCNGG	5 cut(s)	90, 415, 598, 669, 1013
SduI	GDGCHC	3 cut(s)	240, 545, 629
SfaNI	GCATC	3 cut(s)	707, 844, 1053
SinI	GGWCC	1 cut(s)	417
SmiMI	CAYNNNNRTG	1 cut(s)	457
SmlI	CTYRAG	4 cut(s)	71, 1051, 1442, 1477
SmoI	CTYRAG	4 cut(s)	71, 1051, 1442, 1477
Sse9I	AATT	9 cut(s)	362, 378, 386, 500, 796, 996, 1217, 1277, 1389
SsiI	CCGC	9 cut(s)	15, 38, 138, 280, 374, 471, 539, 574, 1416
SspMI	CTAG	1 cut(s)	1490
StyD4I	CCNGG	5 cut(s)	88, 413, 596, 667, 1011
StyI	CCWWGG	1 cut(s)	132
TaaI	ACNGT	4 cut(s)	613, 631, 967, 1192
TaiI	ACGT	2 cut(s)	180, 524
TaqI	TCGA	8 cut(s)	63, 126, 165, 430, 492, 691, 1381, 1519
TasI	AATT	9 cut(s)	362, 378, 386, 500, 796, 996, 1217, 1277, 1389
TatI	WGTACW	1 cut(s)	507
TauI	GCSGC	2 cut(s)	140, 1419
TfiI	GAWTC	1 cut(s)	1312
Tru1I	TTAA	3 cut(s)	747, 1181, 1241
Tru9I	TTAA	3 cut(s)	747, 1181, 1241
TscAI	CASTG	5 cut(s)	427, 517, 564, 889, 1377
TseFI	GTSAC	3 cut(s)	268, 409, 605
TseI	GCWGC	4 cut(s)	274, 1172, 1397, 1400
Tsp45I	GTSAC	3 cut(s)	268, 409, 605
TspDTI	ATGAA	6 cut(s)	151, 280, 744, 1053, 1290, 1304
TspGWI	ACGGA	1 cut(s)	1132
TspRI	CASTG	5 cut(s)	427, 517, 564, 889, 1377
Tth111I	GACNNNGTC	1 cut(s)	611
Van91I	CCANNNNNTGG	1 cut(s)	181
VpaK11BI	GGWCC	1 cut(s)	417
XagI	CCTNNNNNAGG	1 cut(s)	203
XapI	RAATTY	2 cut(s)	1217, 1277
XmiI	GTMKAC	2 cut(s)	820, 1118
XspI	CTAG	1 cut(s)	1490
ZraI	GACGTC	1 cut(s)	522

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.