pycom05g22110

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0016787
Physical Location & Seq
Forward (+)
24612448 .. 24613218
771 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g22110.1

Sequence Viewer

Length: 771 bp
ATGACAATGAAATCAGTTCAGAAACCATTGCAAATCCTCTCCCGTGCCTCAAAATTCACACCCACACCGCTCTCATTTTCCATCTCCTCTTCAGCTTCTGAGTCAATCACTGCACCAAAACCAACCAAACAACCCCAACCCGAAAGCCTAACCCAAGAAGATCTCACAAAGATCAACCTCCTCCTCCCGCGCCTCTGCCTCTTGAACCACCTCGACACGGCGACCCACCTCGCCATCACCGCCCTGCTCGCAAACCCACCTCTCGAATCCGTCTCTCTCTCCGTCCTCATCCACTCCTTCACTTCCCAACCCGACCTGGCCCGACCCATGTCGCTTTTGACCCGCCTCAGACACAACCCGCCTTCGCATCCCCACCTTACGCCTATCGCAACCATGCTCATTGCCTCATACTTCAAGAGAAACAGACCCAGAGAGGCATTCAAGATGTTCAGCTGGTTGGTGAGGCCAGGGTCCCAGTGTGTGCTCGATGAGAGAGTTTGTGAGGTTTTGGTTAATGGGTTTTGTAGGAAAGGGATGGTTTGGGAGGGTTTGAAGGTTTTGAGGGCAATGCTGGGTGCGAATATCGTGCCGGGAGGCGGTGTGAGGAAGTGGGTTTATAGGGGGTTGTTGAGGGAGGCTAGGATTAAGGAGGCAGTGGAGTTGAATGAGGCTTTGGGGTGTGTTGGGGTTGGGGCGAATGGCGATGAATCAGAGGGCGTGAAGGAGGTTTTGGCTTTGTTAGATCACATGCTTTTCAATTGGACAGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR

Protein Analysis

257

Amino Acids

28.31

Weight (kDa)

9.32

Isoelectric Point (pI)

33.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016787)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g13150 FvH4_3g13150
malus_domestica MD05G1244600.v1.1
prunus_persica Prupe.4G119000_v2.0.a1
pyrus_communis pycom05g22110
rosa_chinensis RchiOBHm_Chr5g0021391
rosa_laevigata RLG00000032609
rosa_roxburghii Rroxscaffold_1G00057150
rosa_rugosa Rorug05G0066900
rosa_samantha Rh5AG156000 Rh5BG155500 Rh5CG169200 Rh5DG156000
rosa_wichuraiana Rw5G013890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 70
AccII CGCG 1 cut(s) 190
AciI CCGC 6 cut(s) 68, 188, 240, 343, 359, 597
AcsI RAATTY 1 cut(s) 53
AcuI CTGAAG 1 cut(s) 75
AfiI CCNNNNNNNGG 2 cut(s) 217, 596
AgsI TTSAA 6 cut(s) 205, 415, 442, 553, 664, 757
AjnI CCWGG 2 cut(s) 315, 466
AjuI GAANNNNNNNTTGG 4 cut(s) 656, 688, 713, 745
AluBI AGCT 2 cut(s) 95, 453
AluI AGCT 2 cut(s) 95, 453
Alw21I GWGCWC 1 cut(s) 486
Alw26I GTCTC 1 cut(s) 277
AlwNI CAGNNNCTG 1 cut(s) 98
AoxI GGCC 2 cut(s) 318, 464
ApoI RAATTY 1 cut(s) 53
AspLEI GCGC 1 cut(s) 192
AspS9I GGNCC 2 cut(s) 319, 471
AsuC2I CCSGG 1 cut(s) 591
AsuHPI GGTGA 2 cut(s) 229, 472
AvaII GGWCC 1 cut(s) 471
Bbv12I GWGCWC 1 cut(s) 486
BccI CCATC 3 cut(s) 89, 242, 529
BceAI ACGGC 1 cut(s) 234
BcgI CGANNNNNNTGC 2 cut(s) 568, 602
BciT130I CCWGG 2 cut(s) 317, 468
BcnI CCSGG 1 cut(s) 591
BcoDI GTCTC 1 cut(s) 277
BfaI CTAG 1 cut(s) 639
BglII AGATCT 1 cut(s) 160
Bme1390I CCNGG 3 cut(s) 317, 468, 591
Bme18I GGWCC 1 cut(s) 471
BmgT120I GGNCC 2 cut(s) 319, 471
BmiI GGNNCC 2 cut(s) 472, 473
BmrFI CCNGG 3 cut(s) 317, 468, 591
BmrI ACTGGG 1 cut(s) 469
BmsI GCATC 1 cut(s) 376
BmuI ACTGGG 1 cut(s) 469
BoxI GACNNNNGTC 1 cut(s) 328
BpuMI CCSGG 1 cut(s) 591
BsaJI CCNNGG 1 cut(s) 467
BsaXI ACNNNNNCTCC 2 cut(s) 585, 615
Bsc4I CCNNNNNNNGG 2 cut(s) 217, 596
Bse1I ACTGG 1 cut(s) 475
Bse3DI GCAATG 3 cut(s) 26, 399, 573
BseBI CCWGG 2 cut(s) 317, 468
BseDI CCNNGG 1 cut(s) 467
BseGI GGATG 3 cut(s) 288, 367, 540
BseLI CCNNNNNNNGG 2 cut(s) 217, 596
BseMI GCAATG 3 cut(s) 26, 399, 573
BseMII CTCAG 2 cut(s) 90, 361
BseNI ACTGG 1 cut(s) 475
BseRI GAGGAG 3 cut(s) 76, 170, 173
BseYI CCCAGC 1 cut(s) 571
BsgI GTGCAG 1 cut(s) 96
Bsh1236I CGCG 1 cut(s) 190
BshFI GGCC 2 cut(s) 320, 466
BsiHKAI GWGCWC 1 cut(s) 486
BsiSI CCGG 1 cut(s) 590
BslFI GGGAC 1 cut(s) 457
BslI CCNNNNNNNGG 2 cut(s) 217, 596
BsmAI GTCTC 1 cut(s) 277
BsmBI CGTCTC 1 cut(s) 277
BsmFI GGGAC 1 cut(s) 457
BsmI GAATGC 1 cut(s) 437
BsnI GGCC 2 cut(s) 320, 466
Bsp1286I GDGCHC 1 cut(s) 486
Bsp143I GATC 3 cut(s) 160, 171, 742
BspACI CCGC 6 cut(s) 68, 188, 240, 343, 359, 597
BspANI GGCC 2 cut(s) 320, 466
BspCNI CTCAG 2 cut(s) 91, 360
BspFNI CGCG 1 cut(s) 190
BspLI GGNNCC 2 cut(s) 472, 473
BsrBI CCGCTC 1 cut(s) 70
BsrDI GCAATG 3 cut(s) 26, 399, 573
BsrI ACTGG 1 cut(s) 475
BssECI CCNNGG 1 cut(s) 467
BssMI GATC 3 cut(s) 160, 171, 742
Bst2UI CCWGG 2 cut(s) 317, 468
Bst6I CTCTTC 1 cut(s) 94
BstC8I GCNNGC 1 cut(s) 249
BstDEI CTNAG 2 cut(s) 99, 347
BstF5I GGATG 3 cut(s) 288, 367, 540
BstFNI CGCG 1 cut(s) 190
BstHHI GCGC 1 cut(s) 192
BstKTI GATC 3 cut(s) 163, 174, 745
BstMAI GTCTC 1 cut(s) 277
BstMBI GATC 3 cut(s) 160, 171, 742
BstMWI GCNNNNNNNGC 2 cut(s) 239, 248
BstNI CCWGG 2 cut(s) 317, 468
BstNSI RCATGY 1 cut(s) 751
BstPAI GACNNNNGTC 1 cut(s) 328
BstSCI CCNGG 3 cut(s) 315, 466, 589
BstUI CGCG 1 cut(s) 190
BstX2I RGATCY 1 cut(s) 160
BstYI RGATCY 1 cut(s) 160
BsuRI GGCC 2 cut(s) 320, 466
BtgZI GCGATG 1 cut(s) 717
BtsCI GGATG 3 cut(s) 288, 367, 540
BtsI GCAGTG 2 cut(s) 108, 660
BtsIMutI CAGTG 3 cut(s) 108, 482, 660
Cac8I GCNNGC 1 cut(s) 249
CaiI CAGNNNCTG 1 cut(s) 98
CfoI GCGC 1 cut(s) 192
Cfr13I GGNCC 2 cut(s) 319, 471
CviAII CATG 3 cut(s) 328, 394, 748
CviJI RGCY 8 cut(s) 95, 147, 320, 453, 466, 638, 671, 734
CviKI_1 RGCY 8 cut(s) 95, 147, 320, 453, 466, 638, 671, 734
DdeI CTNAG 2 cut(s) 99, 347
DpnI GATC 3 cut(s) 162, 173, 744
DpnII GATC 3 cut(s) 160, 171, 742
Eam1104I CTCTTC 1 cut(s) 94
EarI CTCTTC 1 cut(s) 94
Eco47I GGWCC 1 cut(s) 471
Eco57I CTGAAG 1 cut(s) 75
EcoO109I RGGNCCY 1 cut(s) 471
EcoRII CCWGG 2 cut(s) 315, 466
Esp3I CGTCTC 1 cut(s) 277
FaeI CATG 3 cut(s) 331, 397, 751
FaiI YATR 5 cut(s) 329, 395, 409, 618, 749
FaqI GGGAC 1 cut(s) 457
FatI CATG 3 cut(s) 327, 393, 747
FauI CCCGC 3 cut(s) 195, 350, 366
FokI GGATG 3 cut(s) 275, 354, 547
FspBI CTAG 1 cut(s) 639
GlaI GCGC 1 cut(s) 191
GsaI CCCAGC 1 cut(s) 575
HaeIII GGCC 2 cut(s) 320, 466
HapII CCGG 1 cut(s) 590
HhaI GCGC 1 cut(s) 192
Hin1II CATG 3 cut(s) 331, 397, 751
Hin6I GCGC 1 cut(s) 190
HinP1I GCGC 1 cut(s) 190
HinfI GANTC 3 cut(s) 101, 266, 707
HpaII CCGG 1 cut(s) 590
HphI GGTGA 2 cut(s) 229, 472
Hpy188I TCNGA 4 cut(s) 21, 100, 350, 712
Hpy188III TCNNGA 4 cut(s) 202, 263, 415, 442
HpyAV CCTTC 4 cut(s) 307, 372, 547, 715
HpyCH4V TGCA 2 cut(s) 31, 113
HpyF10VI GCNNNNNNNGC 2 cut(s) 239, 248
HpyF3I CTNAG 2 cut(s) 99, 347
Hsp92II CATG 3 cut(s) 331, 397, 751
HspAI GCGC 1 cut(s) 190
KflI GGGWCCC 1 cut(s) 471
Kzo9I GATC 3 cut(s) 160, 171, 742
LweI GCATC 1 cut(s) 376
MaeI CTAG 1 cut(s) 639
MalI GATC 3 cut(s) 162, 173, 744
MbiI CCGCTC 1 cut(s) 70
MboI GATC 3 cut(s) 160, 171, 742
MboII GAAGA 2 cut(s) 81, 170
MfeI CAATTG 1 cut(s) 757
MflI RGATCY 1 cut(s) 160
MhlI GDGCHC 1 cut(s) 486
MluCI AATT 2 cut(s) 53, 757
MlyI GAGTC 1 cut(s) 110
MseI TTAA 2 cut(s) 513, 645
MspA1I CMGCKG 1 cut(s) 453
MspI CCGG 1 cut(s) 590
MspR9I CCNGG 3 cut(s) 317, 468, 591
MunI CAATTG 1 cut(s) 757
Mva1269I GAATGC 1 cut(s) 437
MvaI CCWGG 2 cut(s) 317, 468
MvnI CGCG 1 cut(s) 190
MwoI GCNNNNNNNGC 2 cut(s) 239, 248
NciI CCSGG 1 cut(s) 591
NdeII GATC 3 cut(s) 160, 171, 742
NlaIII CATG 3 cut(s) 331, 397, 751
NlaIV GGNNCC 2 cut(s) 472, 473
NspI RCATGY 1 cut(s) 751
PctI GAATGC 1 cut(s) 437
PfeI GAWTC 2 cut(s) 266, 707
PleI GAGTC 1 cut(s) 109
PpsI GAGTC 1 cut(s) 109
PpuMI RGGWCCY 1 cut(s) 471
PshAI GACNNNNGTC 1 cut(s) 328
Psp5II RGGWCCY 1 cut(s) 471
Psp6I CCWGG 2 cut(s) 315, 466
PspFI CCCAGC 1 cut(s) 571
PspGI CCWGG 2 cut(s) 315, 466
PspN4I GGNNCC 2 cut(s) 472, 473
PspPI GGNCC 2 cut(s) 319, 471
PspPPI RGGWCCY 1 cut(s) 471
PstNI CAGNNNCTG 1 cut(s) 98
PsuI RGATCY 1 cut(s) 160
PvuII CAGCTG 1 cut(s) 453
SaqAI TTAA 2 cut(s) 513, 645
Sau3AI GATC 3 cut(s) 160, 171, 742
Sau96I GGNCC 2 cut(s) 319, 471
SchI GAGTC 1 cut(s) 110
ScrFI CCNGG 3 cut(s) 317, 468, 591
SduI GDGCHC 1 cut(s) 486
SfaNI GCATC 1 cut(s) 376
SinI GGWCC 1 cut(s) 471
Sse9I AATT 2 cut(s) 53, 757
SsiI CCGC 6 cut(s) 68, 188, 240, 343, 359, 597
SspMI CTAG 1 cut(s) 639
StyD4I CCNGG 3 cut(s) 315, 466, 589
TaqI TCGA 3 cut(s) 213, 264, 486
TasI AATT 2 cut(s) 53, 757
TfiI GAWTC 2 cut(s) 266, 707
Tru1I TTAA 2 cut(s) 513, 645
Tru9I TTAA 2 cut(s) 513, 645
TscAI CASTG 3 cut(s) 115, 482, 660
TspDTI ATGAA 2 cut(s) 23, 720
TspGWI ACGGA 2 cut(s) 259, 271
TspRI CASTG 3 cut(s) 115, 482, 660
VpaK11BI GGWCC 1 cut(s) 471
XapI RAATTY 1 cut(s) 53
XceI RCATGY 1 cut(s) 751
XspI CTAG 1 cut(s) 639
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.