pycom05g25900

Nucleoporin protein Ndc1-Nup

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0014392
Physical Location & Seq
Reverse (-)
27198566 .. 27201982
3417 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g25900.2

Sequence Viewer

Length: 1647 bp
ATGCCGCCTCCAGCTTCGGCGACGCCGCCGGAGATGGTGCTCCACAACCGCTTCTTAGGGTTCCTCATATGGCAATCCATCCCCTCCACCGTCATCTTCTTCCTCTTCAAAACCCTCTCATCAGTCGTCTTCTCCGCTTCCCCCTCCGCCGACAAGCCCCCTTTCACATTCCCGTTCGCCCCATCGATATTTGCCCTTATTACGTTTTTGACCTTCCACCTCTCCCAGCTCCTCTTCTCCTTCTCACTCTCTCTCGTCGCCTCGCCACACCCCCACCGCCCCGCCTCTATTCTCCAGCTCGTTCTCGGCCTCGTTCGCTTCCTTTTTGTACCGGGTGGCTCCGATTCGTCGGAGACGGCCGATTCCCGTGCCCGGGCTAAGCTTTCGGTGGGTTTCCTGATGTTTTTGGGCGCGGCGGCGGTGTCGGGGTTTGTTTCGGTGGCGTCGTTGTGTGGGGGGTTTGGCGATGGGGTTTCTGCGATTGGGAGAGTAGGGTTTAGGGGTTTTGTGATGGGTTTGCTTTATGGGCTGTACTATGTGTATAATCGCAGGTGGGTCTTGGAGTTTCCCATTATCCAGCGTCCTCCTTTTTTCAGCTTCAAAATGGGGCTTCCTTCAGCTATCACACGATCCTTGCAGCTTTCCTTTGTTGCGTACCTGTTTTCAGCTGCACTGCTAGTGTTTCTGCCCCACCACATTCAGAACCAGACTACAACTGGAAAGTTTATTGCCGAGCAGATCAGGTTTTACATTGGGAGTTTCTTAGTGTTTCTCTGCTGGGAACTAAGTCACCATTTACATCGGGTGCTTCACACAAAGAGATTCGTATTTGCGCCACCTAAAGGATCAGCAGCAGCAGAAACAAATCCAAGTGAGATTCTCCTTGCAGCTCTGGAGGAGAGCAGTCCTAATTCTCTTCTTCAGTATCTTGCATATCTTGATCTCTGTATGGTTTGCGAGAATAATGTGGATGCTTGGAGAAGAGCTGCATTTTTTGAGGAAACTGGTGAAACGTACAGAAGAGTTATATCTGTATGCTTGCGGCCTCTGGAGCAGCTTGCATCAAAGCTAGGTGAAGGCTTGGAAAGTTCCATTGAAAACGGCTCCCAAATATCCAATCAGTTACTGTCCCCAGCTGACAACCGACTAGACTCAAAATACCCTGAAGCCTTGAATAACTTCCAGCTATATGCATGGTGTGCGTGGACAGCTGCCTCACTGACTGCATGCTCACTCAAAGAGGACAGATATGGGGTTGCCCAGATCTCTGGTAGTAATGCTGCTGTAATGTCCACACTGATTTCTTGCCTTCTTGCTGTTGAAACATACATGGGGAAGAAGACCACTTTGCAGTCTGCTAATCAGTTCATGGGGTCAACTGGTTTTAAATTGAGAAACTCGAGCATGGCTAATACTGGCACAAGCAAAAAGAGAGCCGGCCCGCTTCATTCAAAAGCATATGCTGTTGCTGATGTTCTGAGGAACTCGATCTACCAGATTGTGTCTGCTTTCCATGATCAGATGGCAAGCAGTGCTAAAAAGGGCTTTCTTGACAAGGAATGGATCATTAACAGCAAGCCTCCTTTCGGACCACGCGAACTTCTTGTGCAGAAACTGCGTCTATTCCTTGACTTTCGAGCCAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
Ndc1_Nup

Protein Analysis

549

Amino Acids

60.43

Weight (kDa)

9.34

Isoelectric Point (pI)

47.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014392)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G73240
fragaria_vesca FvH4_3g04970
malus_domestica MD05G1278400.v1.1 MD10G1257000.v1.1
prunus_persica Prupe.4G086400_v2.0.a1
pyrus_communis pycom05g25900 pycom10g21270
rosa_chinensis RchiOBHm_Chr5g0015011
rosa_laevigata RLG00000032160
rosa_multiflora Rmu_sc0004889.1_g000023
rosa_roxburghii Rroxscaffold_1G00061530
rosa_rugosa Rorug05G0022800
rosa_samantha Rh5AG118000 Rh5BG115200 Rh5CG126500 Rh5DG114700
rosa_wichuraiana Rw5G010170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 540
Acc36I ACCTGC 1 cut(s) 540
AccII CGCG 2 cut(s) 413, 1596
AclWI GGATC 3 cut(s) 624, 853, 1571
AcoI YGGCCR 1 cut(s) 357
AcuI CTGAAG 3 cut(s) 600, 905, 1185
AcyI GRCGYC 2 cut(s) 23, 443
AfaI GTAC 4 cut(s) 330, 533, 656, 1016
AfiI CCNNNNNNNGG 4 cut(s) 372, 373, 842, 1586
AgsI TTSAA 6 cut(s) 109, 601, 1097, 1174, 1322, 1452
Alw21I GWGCWC 1 cut(s) 42
Alw26I GTCTC 1 cut(s) 347
AlwI GGATC 3 cut(s) 624, 853, 1571
AlwNI CAGNNNCTG 2 cut(s) 1126, 1615
Ama87I CYCGRG 2 cut(s) 372, 1399
AoxI GGCC 4 cut(s) 307, 357, 1043, 1438
ApeKI GCWGC 9 cut(s) 637, 668, 851, 854, 887, 986, 1054, 1211, 1280
Asp700I GAANNNNTTC 1 cut(s) 1178
AspLEI GCGC 2 cut(s) 413, 835
AspS9I GGNCC 2 cut(s) 1439, 1589
AsuC2I CCSGG 3 cut(s) 333, 373, 374
AsuHPI GGTGA 3 cut(s) 782, 1019, 1085
AvaI CYCGRG 2 cut(s) 372, 1399
AvaII GGWCC 1 cut(s) 1589
BaeGI GKGCMC 1 cut(s) 373
BbsI GAAGAC 2 cut(s) 121, 1346
Bbv12I GWGCWC 1 cut(s) 42
BbvI GCAGC 9 cut(s) 649, 655, 863, 866, 899, 973, 1066, 1198, 1267
BccI CCATC 6 cut(s) 28, 86, 190, 461, 505, 1516
BceAI ACGGC 2 cut(s) 372, 1117
BcgI CGANNNNNNTGC 2 cut(s) 350, 384
BclI TGATCA 1 cut(s) 1516
BcnI CCSGG 3 cut(s) 333, 373, 374
BcoDI GTCTC 1 cut(s) 347
BfaI CTAG 3 cut(s) 677, 1070, 1148
BfuAI ACCTGC 1 cut(s) 540
BglII AGATCT 1 cut(s) 1263
BlpI GCTNAGC 1 cut(s) 378
Bme1390I CCNGG 3 cut(s) 333, 373, 374
Bme18I GGWCC 1 cut(s) 1589
BmeT110I CYCGRG 2 cut(s) 372, 1399
BmgT120I GGNCC 2 cut(s) 1439, 1589
BmiI GGNNCC 3 cut(s) 62, 340, 1105
BmrFI CCNGG 3 cut(s) 333, 373, 374
BmsI GCATC 2 cut(s) 961, 1070
BpiI GAAGAC 2 cut(s) 121, 1346
BpmI CTGGAG 3 cut(s) 278, 914, 1070
Bpu1102I GCTNAGC 1 cut(s) 378
BpuMI CCSGG 3 cut(s) 333, 373, 374
Bsa29I ATCGAT 1 cut(s) 185
BsaHI GRCGYC 2 cut(s) 23, 443
BsaJI CCNNGG 1 cut(s) 372
Bsc4I CCNNNNNNNGG 4 cut(s) 372, 373, 842, 1586
Bse118I RCCGGY 1 cut(s) 1436
Bse1I ACTGG 4 cut(s) 721, 1009, 1384, 1420
BseCI ATCGAT 1 cut(s) 185
BseDI CCNNGG 1 cut(s) 372
BseGI GGATG 2 cut(s) 78, 976
BseLI CCNNNNNNNGG 4 cut(s) 372, 373, 842, 1586
BseMII CTCAG 1 cut(s) 1469
BseNI ACTGG 4 cut(s) 721, 1009, 1384, 1420
BseRI GAGGAG 2 cut(s) 221, 911
BseSI GKGCMC 1 cut(s) 373
BseX3I CGGCCG 1 cut(s) 357
BseXI GCAGC 9 cut(s) 649, 655, 863, 866, 899, 973, 1066, 1198, 1267
BseYI CCCAGC 3 cut(s) 225, 777, 1132
BsgI GTGCAG 2 cut(s) 654, 1628
Bsh1236I CGCG 2 cut(s) 413, 1596
Bsh1285I CGRYCG 1 cut(s) 360
BshFI GGCC 4 cut(s) 309, 359, 1045, 1440
BshVI ATCGAT 1 cut(s) 185
BsiEI CGRYCG 1 cut(s) 360
BsiHKAI GWGCWC 1 cut(s) 42
BsiHKCI CYCGRG 2 cut(s) 372, 1399
BsiSI CCGG 4 cut(s) 29, 332, 373, 1437
BslFI GGGAC 1 cut(s) 1114
BslI CCNNNNNNNGG 4 cut(s) 372, 373, 842, 1586
BsmAI GTCTC 1 cut(s) 347
BsmBI CGTCTC 1 cut(s) 347
BsmFI GGGAC 1 cut(s) 1114
BsnI GGCC 4 cut(s) 309, 359, 1045, 1440
BsoBI CYCGRG 2 cut(s) 372, 1399
Bsp1286I GDGCHC 2 cut(s) 42, 373
Bsp143I GATC 8 cut(s) 629, 738, 845, 940, 1263, 1488, 1516, 1563
Bsp1720I GCTNAGC 1 cut(s) 378
BspANI GGCC 4 cut(s) 309, 359, 1045, 1440
BspCNI CTCAG 1 cut(s) 1470
BspDI ATCGAT 1 cut(s) 185
BspFNI CGCG 2 cut(s) 413, 1596
BspLI GGNNCC 3 cut(s) 62, 340, 1105
BspMI ACCTGC 1 cut(s) 540
BspPI GGATC 3 cut(s) 624, 853, 1571
BspQI GCTCTTC 1 cut(s) 976
BsrFI RCCGGY 1 cut(s) 1436
BsrI ACTGG 4 cut(s) 721, 1009, 1384, 1420
BssAI RCCGGY 1 cut(s) 1436
BssECI CCNNGG 1 cut(s) 372
BssMI GATC 8 cut(s) 629, 738, 845, 940, 1263, 1488, 1516, 1563
BssNI GRCGYC 2 cut(s) 23, 443
Bst4CI ACNGT 2 cut(s) 91, 1128
Bst6I CTCTTC 5 cut(s) 110, 239, 921, 976, 1015
BstACI GRCGYC 2 cut(s) 23, 443
BstAPI GCANNNNNTGC 3 cut(s) 1199, 1532, 1615
BstC8I GCNNGC 8 cut(s) 1040, 1059, 1228, 1438, 1442, 1528, 1577, 1642
BstDEI CTNAG 5 cut(s) 55, 378, 763, 785, 1478
BstF5I GGATG 2 cut(s) 78, 976
BstFNI CGCG 2 cut(s) 413, 1596
BstHHI GCGC 2 cut(s) 413, 835
BstKTI GATC 8 cut(s) 632, 741, 848, 943, 1266, 1491, 1519, 1566
BstMAI GTCTC 1 cut(s) 347
BstMBI GATC 8 cut(s) 629, 738, 845, 940, 1263, 1488, 1516, 1563
BstMCI CGRYCG 1 cut(s) 360
BstMWI GCNNNNNNNGC 7 cut(s) 315, 526, 1051, 1199, 1208, 1532, 1615
BstNSI RCATGY 1 cut(s) 1230
BstSCI CCNGG 3 cut(s) 331, 371, 372
BstSLI GKGCMC 1 cut(s) 373
BstUI CGCG 2 cut(s) 413, 1596
BstV1I GCAGC 9 cut(s) 649, 655, 863, 866, 899, 973, 1066, 1198, 1267
BstV2I GAAGAC 2 cut(s) 121, 1346
BstX2I RGATCY 1 cut(s) 1263
BstXI CCANNNNNNTGG 1 cut(s) 1268
BstYI RGATCY 1 cut(s) 1263
BstZI CGGCCG 1 cut(s) 357
Bsu15I ATCGAT 1 cut(s) 185
BsuRI GGCC 4 cut(s) 309, 359, 1045, 1440
BsuTUI ATCGAT 1 cut(s) 185
BtgZI GCGATG 1 cut(s) 480
BtsCI GGATG 2 cut(s) 78, 976
BtsI GCAGTG 2 cut(s) 671, 1537
BtsIMutI CAGTG 4 cut(s) 671, 1217, 1295, 1537
BveI ACCTGC 1 cut(s) 540
Cac8I GCNNGC 8 cut(s) 1040, 1059, 1228, 1438, 1442, 1528, 1577, 1642
CaiI CAGNNNCTG 2 cut(s) 1126, 1615
CfoI GCGC 2 cut(s) 413, 835
Cfr10I RCCGGY 1 cut(s) 1436
Cfr13I GGNCC 2 cut(s) 1439, 1589
Cfr9I CCCGGG 1 cut(s) 372
ClaI ATCGAT 1 cut(s) 185
CseI GACGC 4 cut(s) 31, 432, 569, 1607
Csp6I GTAC 4 cut(s) 329, 532, 655, 1015
CviAII CATG 6 cut(s) 1194, 1227, 1330, 1369, 1405, 1514
CviQI GTAC 4 cut(s) 329, 532, 655, 1015
DdeI CTNAG 5 cut(s) 55, 378, 763, 785, 1478
DpnI GATC 8 cut(s) 631, 740, 847, 942, 1265, 1490, 1518, 1565
DpnII GATC 8 cut(s) 629, 738, 845, 940, 1263, 1488, 1516, 1563
DraI TTTAAA 1 cut(s) 1387
EaeI YGGCCR 1 cut(s) 357
EagI CGGCCG 1 cut(s) 357
Eam1104I CTCTTC 5 cut(s) 110, 239, 921, 976, 1015
EarI CTCTTC 5 cut(s) 110, 239, 921, 976, 1015
EciI GGCGGA 1 cut(s) 136
EclXI CGGCCG 1 cut(s) 357
Eco47I GGWCC 1 cut(s) 1589
Eco52I CGGCCG 1 cut(s) 357
Eco57I CTGAAG 3 cut(s) 600, 905, 1185
Eco88I CYCGRG 2 cut(s) 372, 1399
EcoT22I ATGCAT 1 cut(s) 1195
Esp3I CGTCTC 1 cut(s) 347
FaeI CATG 6 cut(s) 1197, 1230, 1333, 1372, 1408, 1517
FaqI GGGAC 1 cut(s) 1114
FatI CATG 6 cut(s) 1193, 1226, 1329, 1368, 1404, 1513
FauI CCCGC 2 cut(s) 289, 1449
FauNDI CATATG 2 cut(s) 68, 1459
FbaI TGATCA 1 cut(s) 1516
FokI GGATG 2 cut(s) 65, 983
FspBI CTAG 3 cut(s) 677, 1070, 1148
GlaI GCGC 2 cut(s) 412, 834
GsaI CCCAGC 3 cut(s) 229, 781, 1136
GsuI CTGGAG 3 cut(s) 278, 914, 1070
HaeIII GGCC 4 cut(s) 309, 359, 1045, 1440
HapII CCGG 4 cut(s) 29, 332, 373, 1437
HgaI GACGC 4 cut(s) 31, 432, 569, 1607
HhaI GCGC 2 cut(s) 413, 835
Hin1I GRCGYC 2 cut(s) 23, 443
Hin1II CATG 6 cut(s) 1197, 1230, 1333, 1372, 1408, 1517
Hin6I GCGC 2 cut(s) 411, 833
HinP1I GCGC 2 cut(s) 411, 833
HincII GTYRAC 1 cut(s) 1377
HindII GTYRAC 1 cut(s) 1377
HindIII AAGCTT 1 cut(s) 380
HinfI GANTC 5 cut(s) 344, 362, 822, 877, 1151
HpaII CCGG 4 cut(s) 29, 332, 373, 1437
HphI GGTGA 3 cut(s) 782, 1019, 1085
Hpy166II GTNNAC 3 cut(s) 1206, 1293, 1377
Hpy188I TCNGA 6 cut(s) 343, 352, 702, 1479, 1521, 1589
Hpy188III TCNNGA 5 cut(s) 397, 893, 938, 1049, 1550
Hpy8I GTNNAC 3 cut(s) 1206, 1293, 1377
Hpy99I CGWCG 4 cut(s) 25, 260, 352, 448
HpyAV CCTTC 5 cut(s) 223, 250, 624, 1070, 1319
HpyCH4III ACNGT 2 cut(s) 91, 1128
HpyCH4IV ACGT 2 cut(s) 203, 1013
HpyF10VI GCNNNNNNNGC 7 cut(s) 315, 526, 1051, 1199, 1208, 1532, 1615
HpyF3I CTNAG 5 cut(s) 55, 378, 763, 785, 1478
HpySE526I ACGT 2 cut(s) 203, 1013
Hsp92I GRCGYC 2 cut(s) 23, 443
Hsp92II CATG 6 cut(s) 1197, 1230, 1333, 1372, 1408, 1517
HspAI GCGC 2 cut(s) 411, 833
KroI GCCGGC 1 cut(s) 1436
KroNI GCCGGC 1 cut(s) 1438
Ksp22I TGATCA 1 cut(s) 1516
Kzo9I GATC 8 cut(s) 629, 738, 845, 940, 1263, 1488, 1516, 1563
LguI GCTCTTC 1 cut(s) 976
LmnI GCTCC 5 cut(s) 45, 234, 344, 1051, 1109
Lsp1109I GCAGC 9 cut(s) 649, 655, 863, 866, 899, 973, 1066, 1198, 1267
LweI GCATC 2 cut(s) 961, 1070
MaeI CTAG 3 cut(s) 677, 1070, 1148
MaeII ACGT 2 cut(s) 203, 1013
MaeIII GTNAC 2 cut(s) 788, 1122
MalI GATC 8 cut(s) 631, 740, 847, 942, 1265, 1490, 1518, 1565
MboI GATC 8 cut(s) 629, 738, 845, 940, 1263, 1488, 1516, 1563
MflI RGATCY 1 cut(s) 1263
MhlI GDGCHC 2 cut(s) 42, 373
MluCI AATT 2 cut(s) 910, 1388
MlyI GAGTC 1 cut(s) 1145
MmeI TCCRAC 1 cut(s) 330
Mph1103I ATGCAT 1 cut(s) 1195
MroNI GCCGGC 1 cut(s) 1436
MroXI GAANNNNTTC 1 cut(s) 1178
MseI TTAA 2 cut(s) 1386, 1569
MspA1I CMGCKG 3 cut(s) 668, 1136, 1211
MspI CCGG 4 cut(s) 29, 332, 373, 1437
MspR9I CCNGG 3 cut(s) 333, 373, 374
MvnI CGCG 2 cut(s) 413, 1596
MwoI GCNNNNNNNGC 7 cut(s) 315, 526, 1051, 1199, 1208, 1532, 1615
NaeI GCCGGC 1 cut(s) 1438
NciI CCSGG 3 cut(s) 333, 373, 374
NdeI CATATG 2 cut(s) 68, 1459
NdeII GATC 8 cut(s) 629, 738, 845, 940, 1263, 1488, 1516, 1563
NgoMIV GCCGGC 1 cut(s) 1436
NlaIII CATG 6 cut(s) 1197, 1230, 1333, 1372, 1408, 1517
NlaIV GGNNCC 3 cut(s) 62, 340, 1105
NmeAIII GCCGAG 2 cut(s) 285, 757
NmuCI GTSAC 1 cut(s) 788
NsiI ATGCAT 1 cut(s) 1195
NspI RCATGY 1 cut(s) 1230
PaeI GCATGC 1 cut(s) 1230
PaeR7I CTCGAG 1 cut(s) 1399
PaqCI CACCTGC 1 cut(s) 540
PciSI GCTCTTC 1 cut(s) 976
PcsI WCGNNNNNNNCGW 2 cut(s) 443, 1593
PdiI GCCGGC 1 cut(s) 1438
PdmI GAANNNNTTC 1 cut(s) 1178
PfeI GAWTC 4 cut(s) 344, 362, 822, 877
PleI GAGTC 1 cut(s) 1145
PpsI GAGTC 1 cut(s) 1145
PspFI CCCAGC 3 cut(s) 225, 777, 1132
PspN4I GGNNCC 3 cut(s) 62, 340, 1105
PspPI GGNCC 2 cut(s) 1439, 1589
PspXI VCTCGAGB 1 cut(s) 1399
PsrI GAACNNNNNNTAC 2 cut(s) 1475, 1507
PstNI CAGNNNCTG 2 cut(s) 1126, 1615
PsuI RGATCY 1 cut(s) 1263
PvuII CAGCTG 3 cut(s) 668, 1136, 1211
RsaI GTAC 4 cut(s) 330, 533, 656, 1016
RsaNI GTAC 4 cut(s) 329, 532, 655, 1015
SapI GCTCTTC 1 cut(s) 976
SaqAI TTAA 2 cut(s) 1386, 1569
Sau3AI GATC 8 cut(s) 629, 738, 845, 940, 1263, 1488, 1516, 1563
Sau96I GGNCC 2 cut(s) 1439, 1589
SchI GAGTC 1 cut(s) 1145
ScrFI CCNGG 3 cut(s) 333, 373, 374
SduI GDGCHC 2 cut(s) 42, 373
SfaNI GCATC 2 cut(s) 961, 1070
Sfr274I CTCGAG 1 cut(s) 1399
SinI GGWCC 1 cut(s) 1589
SlaI CTCGAG 1 cut(s) 1399
SmaI CCCGGG 1 cut(s) 374
SmlI CTYRAG 1 cut(s) 1399
SmoI CTYRAG 1 cut(s) 1399
SphI GCATGC 1 cut(s) 1230
SrfI GCCCGGGC 1 cut(s) 374
Sse9I AATT 2 cut(s) 910, 1388
SspMI CTAG 3 cut(s) 677, 1070, 1148
StyD4I CCNGG 3 cut(s) 331, 371, 372
TaaI ACNGT 2 cut(s) 91, 1128
TaiI ACGT 2 cut(s) 206, 1016
TaqI TCGA 4 cut(s) 185, 1400, 1487, 1636
TasI AATT 2 cut(s) 910, 1388
TatI WGTACW 1 cut(s) 531
TauI GCSGC 5 cut(s) 7, 28, 416, 419, 1045
TfiI GAWTC 4 cut(s) 344, 362, 822, 877
Tru1I TTAA 2 cut(s) 1386, 1569
Tru9I TTAA 2 cut(s) 1386, 1569
TscAI CASTG 4 cut(s) 678, 1224, 1302, 1537
TseFI GTSAC 1 cut(s) 788
TseI GCWGC 9 cut(s) 637, 668, 851, 854, 887, 986, 1054, 1211, 1280
Tsp45I GTSAC 1 cut(s) 788
TspDTI ATGAA 2 cut(s) 1357, 1436
TspMI CCCGGG 1 cut(s) 372
TspRI CASTG 4 cut(s) 678, 1224, 1302, 1537
VpaK11BI GGWCC 1 cut(s) 1589
XceI RCATGY 1 cut(s) 1230
XcmI CCANNNNNNNNNTGG 1 cut(s) 713
XhoI CTCGAG 1 cut(s) 1399
XmaI CCCGGG 1 cut(s) 372
XmnI GAANNNNTTC 1 cut(s) 1178
XspI CTAG 3 cut(s) 677, 1070, 1148
Zsp2I ATGCAT 1 cut(s) 1195
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.