Rh5BG115200

Nucleoporin protein Ndc1-Nup

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0014392
Physical Location & Seq
Forward (+)
10780500 .. 10784084
3585 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG115200.1

Sequence Viewer

Length: 1599 bp
ATGCCTCCAGCGCCGCCGGACGCGGTGGTCCGCAACCGCTTCTTAGGGTTCCTCATCTGGCAATCCATCCCCTCCACCCTCATTTTCCTCCTCTCCGCTCTCTCTCCCTCAAAACCCCCTTTCCCATTCGCCCCATGGTTCTTCGCCGTAATTACCTTCCTGTCCTTCCACCTCTCTCAGCTCCTCTTCTCCGCCTCCCTCTCTCTCATCTCCTCCACCCACCCCCACCACCAACTGCTCTCGCCTCTCAAGCTCTCCCTCGAGCTCATACGCTTCCTTTTCGTTCCCGGCGGCTCCGATTTGACCGGGTCGCCGGGTTCTCGGGTCCGGGTTAGGGTCACCGTCCGCTTCGTGGCGTTTCTGGCTGCGGCGGCGGTGTCCGGGTCAGTTGCGGCGGTTTCGGTCTGCGGGGAGCTCGGTGGTGGGGTTTGGGGGATTGGGAGAGTAGGGTTTAGGGGTTTTGTGATGGGTTTGCTTTATGGGTTGTATTATGTGTATAAGCGCAGGTGGGTCTTGGAGTTCCCCATTATTCAGCGTCCGCCATTTTTTAGCTTCAAGATGGGTCTCCCCACGGCTATCACACGATCCTTAAAGCTGTCCGGTGTGGCATACCTAGTCTCAGCTATTCTGGTGGTGTTCCTTCCCCATCAGTTTCACCACAACAGAGTTACAATTGGAAGGTTGATTACGGAGCAGATTACCTTCTACATTGGGAGTTTCTTGGTGTTTCTCTGCTGGGAACTGAGTCACCATTTACATCAGGTGCTTAATACCAAGAGATGCATATTTGCGCCGCCTAAAGGATCAGCTGCAGCAGAAACAAATCCAACTGAGCATCTTCTTGCAGCTTTGGAGGAGAGCACTCCTAATTCTCTTCCTCAGTATCTTGCATATCTTGATCTCTGCATGATTTGTGAGAATAATGTTGATACTTGGCGACGAGCTGCCTTTTTCGAAGAAAGTGGTGAAACTTACAAAAGGGTTGTATCTGTCTGCTTGCGGCCTCTGGAGCAGCTTGCATCAAAGCTGGGTGATGGCTTGGAAAGTCCTGTTAATGGAAAATCCCAAATATCCAATCAGTTACTGTCGCCAACTGACCAGCGACTAGATTCAAAATACTCTGAACCCTTTGACAACTTCCAGCTATATGCATGGTGTGCCTGGTCAGCTGCTTCACTGACTGCACGCTCAAATACAGAGGACAGATTTGGGGTTGCCCAGAGATCGGGTAGTAATGCTGCTGTGATCTCAACACTGATCTCTTGCCTTCTTGCTGTTGAAACATACATGGGAAAGAAGACCAATGTTCCTGCACAGTTTATGGGGCCAGCTAGTATCAAATGGGCAACCTCGAGTGTGGGAAATGTTGGCGTGGCTAGAAAGAGAGTTGGCCCTATCCATCCCAAAGCATATGCCCTTGCTGATGTTCTTAAAAACTCAATCTACCATATTGTGTCCGCATGCCATGATCAGATGGTGAGCAGTGCCAAAACAGGCGTGCTTGAGAAGTATTGGGTCATTGAAGGCAAGCCTCCTTTTGGGTCACACGAGCTTCTGGTGCAGAAATTGCGTATTTTCCTAGATTTTCGAGCTAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
Ndc1_Nup

Protein Analysis

532

Amino Acids

58.71

Weight (kDa)

9.47

Isoelectric Point (pI)

44.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ndc1_Nup PF09531 153 - 356 8e-16 Nucleoporin protein Ndc1-Nup
Ndc1_Nup PF09531 366 - 487 1.7e-08 Nucleoporin protein Ndc1-Nup
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014392)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G73240
fragaria_vesca FvH4_3g04970
malus_domestica MD05G1278400.v1.1 MD10G1257000.v1.1
prunus_persica Prupe.4G086400_v2.0.a1
pyrus_communis pycom05g25900 pycom10g21270
rosa_chinensis RchiOBHm_Chr5g0015011
rosa_laevigata RLG00000032160
rosa_multiflora Rmu_sc0004889.1_g000023
rosa_roxburghii Rroxscaffold_1G00061530
rosa_rugosa Rorug05G0022800
rosa_samantha Rh5AG118000 Rh5BG115200 Rh5CG126500 Rh5DG114700
rosa_wichuraiana Rw5G010170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 495
AasI GACNNNNNNGTC 1 cut(s) 26
Acc36I ACCTGC 1 cut(s) 495
AccBSI CCGCTC 1 cut(s) 98
AccII CGCG 1 cut(s) 23
AclWI GGATC 2 cut(s) 579, 811
AfiI CCNNNNNNNGG 6 cut(s) 334, 352, 800, 1055, 1225, 1538
AgsI TTSAA 4 cut(s) 556, 1113, 1280, 1523
AjnI CCWGG 1 cut(s) 1160
Alw21I GWGCWC 3 cut(s) 267, 417, 863
Alw26I GTCTC 2 cut(s) 569, 622
AlwI GGATC 2 cut(s) 579, 811
AlwNI CAGNNNCTG 1 cut(s) 1084
Ama87I CYCGRG 3 cut(s) 260, 321, 1351
AoxI GGCC 3 cut(s) 1001, 1325, 1390
ApeKI GCWGC 8 cut(s) 365, 809, 812, 845, 944, 1012, 1169, 1238
AspLEI GCGC 3 cut(s) 13, 504, 793
AspS9I GGNCC 4 cut(s) 28, 325, 1325, 1391
AsuC2I CCSGG 5 cut(s) 288, 307, 315, 329, 382
AsuHPI GGTGA 6 cut(s) 331, 647, 740, 977, 1043, 1489
AsuII TTCGAA 1 cut(s) 954
AsuNHI GCTAGC 1 cut(s) 1592
AvaI CYCGRG 3 cut(s) 260, 321, 1351
AvaII GGWCC 2 cut(s) 28, 325
BanII GRGCYC 2 cut(s) 267, 417
BarI GAAGNNNNNNTAC 2 cut(s) 670, 702
BauI CACGAG 1 cut(s) 1547
BbsI GAAGAC 1 cut(s) 1304
Bbv12I GWGCWC 3 cut(s) 267, 417, 863
BbvI GCAGC 8 cut(s) 352, 796, 824, 857, 931, 1024, 1156, 1225
BccI CCATC 7 cut(s) 74, 460, 553, 654, 1028, 1407, 1468
BceAI ACGGC 2 cut(s) 131, 588
BciT130I CCWGG 1 cut(s) 1162
BclI TGATCA 1 cut(s) 1468
BcnI CCSGG 5 cut(s) 288, 307, 315, 329, 382
BcoDI GTCTC 2 cut(s) 569, 622
BfaI CTAG 6 cut(s) 614, 1106, 1332, 1377, 1580, 1593
BfmI CTRYAG 1 cut(s) 810
BfoI RGCGCY 1 cut(s) 14
BfuAI ACCTGC 1 cut(s) 495
Bme1390I CCNGG 6 cut(s) 288, 307, 315, 329, 382, 1162
Bme18I GGWCC 2 cut(s) 28, 325
BmeT110I CYCGRG 3 cut(s) 260, 321, 1351
BmgT120I GGNCC 4 cut(s) 28, 325, 1325, 1391
BmiI GGNNCC 4 cut(s) 50, 295, 326, 1326
BmrFI CCNGG 6 cut(s) 288, 307, 315, 329, 382, 1162
BmsI GCATC 3 cut(s) 770, 844, 1028
BmtI GCTAGC 1 cut(s) 1596
BpiI GAAGAC 1 cut(s) 1304
BpmI CTGGAG 1 cut(s) 1028
Bpu14I TTCGAA 1 cut(s) 954
BpuEI CTTGAG 2 cut(s) 233, 1523
BpuMI CCSGG 5 cut(s) 288, 307, 315, 329, 382
BsaI GGTCTC 1 cut(s) 569
BsaJI CCNNGG 2 cut(s) 134, 570
BsaWI WCCGGW 1 cut(s) 599
Bsc4I CCNNNNNNNGG 6 cut(s) 334, 352, 800, 1055, 1225, 1538
BseBI CCWGG 1 cut(s) 1162
BseDI CCNNGG 2 cut(s) 134, 570
BseGI GGATG 2 cut(s) 66, 1399
BseLI CCNNNNNNNGG 6 cut(s) 334, 352, 800, 1055, 1225, 1538
BseMII CTCAG 5 cut(s) 191, 633, 734, 822, 893
BseRI GAGGAG 4 cut(s) 80, 173, 202, 869
BseXI GCAGC 8 cut(s) 352, 796, 824, 857, 931, 1024, 1156, 1225
BseYI CCCAGC 2 cut(s) 735, 1027
BsgI GTGCAG 3 cut(s) 1167, 1296, 1580
Bsh1236I CGCG 1 cut(s) 23
BshFI GGCC 3 cut(s) 1003, 1327, 1392
BsiHKAI GWGCWC 3 cut(s) 267, 417, 863
BsiHKCI CYCGRG 3 cut(s) 260, 321, 1351
BsiSI CCGG 7 cut(s) 17, 288, 306, 314, 328, 381, 600
BslI CCNNNNNNNGG 6 cut(s) 334, 352, 800, 1055, 1225, 1538
BsmAI GTCTC 2 cut(s) 569, 622
BsnI GGCC 3 cut(s) 1003, 1327, 1392
Bso31I GGTCTC 1 cut(s) 569
BsoBI CYCGRG 3 cut(s) 260, 321, 1351
Bsp119I TTCGAA 1 cut(s) 954
Bsp1286I GDGCHC 3 cut(s) 267, 417, 863
Bsp143I GATC 7 cut(s) 584, 803, 898, 1223, 1245, 1257, 1468
Bsp19I CCATGG 1 cut(s) 134
BspANI GGCC 3 cut(s) 1003, 1327, 1392
BspCNI CTCAG 5 cut(s) 190, 632, 735, 823, 892
BspFNI CGCG 1 cut(s) 23
BspLI GGNNCC 4 cut(s) 50, 295, 326, 1326
BspMAI CTGCAG 1 cut(s) 814
BspMI ACCTGC 1 cut(s) 495
BspOI GCTAGC 1 cut(s) 1596
BspPI GGATC 2 cut(s) 579, 811
BspT104I TTCGAA 1 cut(s) 954
BspTNI GGTCTC 1 cut(s) 569
BsrBI CCGCTC 1 cut(s) 98
BssECI CCNNGG 2 cut(s) 134, 570
BssMI GATC 7 cut(s) 584, 803, 898, 1223, 1245, 1257, 1468
BssSI CACGAG 1 cut(s) 1547
BssT1I CCWWGG 1 cut(s) 134
Bst2BI CACGAG 1 cut(s) 1547
Bst2UI CCWGG 1 cut(s) 1162
Bst4CI ACNGT 3 cut(s) 343, 1086, 1317
Bst6I CTCTTC 2 cut(s) 191, 879
BstAPI GCANNNNNTGC 2 cut(s) 1157, 1567
BstBI TTCGAA 1 cut(s) 954
BstC8I GCNNGC 8 cut(s) 998, 1017, 1186, 1329, 1462, 1499, 1529, 1594
BstDEI CTNAG 6 cut(s) 43, 177, 619, 743, 831, 879
BstDSI CCRYGG 2 cut(s) 134, 570
BstEII GGTNACC 1 cut(s) 337
BstF5I GGATG 2 cut(s) 66, 1399
BstFNI CGCG 1 cut(s) 23
BstH2I RGCGCY 1 cut(s) 14
BstHHI GCGC 3 cut(s) 13, 504, 793
BstKTI GATC 7 cut(s) 587, 806, 901, 1226, 1248, 1260, 1471
BstMAI GTCTC 2 cut(s) 569, 622
BstMBI GATC 7 cut(s) 584, 803, 898, 1223, 1245, 1257, 1468
BstMWI GCNNNNNNNGC 9 cut(s) 10, 250, 362, 371, 1009, 1157, 1166, 1558, 1567
BstNI CCWGG 1 cut(s) 1162
BstNSI RCATGY 1 cut(s) 1464
BstPI GGTNACC 1 cut(s) 337
BstSCI CCNGG 6 cut(s) 286, 305, 313, 327, 380, 1160
BstSFI CTRYAG 1 cut(s) 810
BstUI CGCG 1 cut(s) 23
BstV1I GCAGC 8 cut(s) 352, 796, 824, 857, 931, 1024, 1156, 1225
BstV2I GAAGAC 1 cut(s) 1304
BsuRI GGCC 3 cut(s) 1003, 1327, 1392
BtgI CCRYGG 2 cut(s) 134, 570
BtsCI GGATG 2 cut(s) 66, 1399
BtsI GCAGTG 1 cut(s) 1489
BtsIMutI CAGTG 3 cut(s) 1175, 1253, 1489
BveI ACCTGC 1 cut(s) 495
Cac8I GCNNGC 8 cut(s) 998, 1017, 1186, 1329, 1462, 1499, 1529, 1594
CaiI CAGNNNCTG 1 cut(s) 1084
CfoI GCGC 3 cut(s) 13, 504, 793
Cfr13I GGNCC 4 cut(s) 28, 325, 1325, 1391
CseI GACGC 2 cut(s) 29, 524
CviAII CATG 6 cut(s) 135, 907, 1152, 1288, 1461, 1466
DdeI CTNAG 6 cut(s) 43, 177, 619, 743, 831, 879
DpnI GATC 7 cut(s) 586, 805, 900, 1225, 1247, 1259, 1470
DpnII GATC 7 cut(s) 584, 803, 898, 1223, 1245, 1257, 1468
DrdI GACNNNNNNGTC 1 cut(s) 26
DseDI GACNNNNNNGTC 1 cut(s) 26
Eam1104I CTCTTC 2 cut(s) 191, 879
EarI CTCTTC 2 cut(s) 191, 879
EciI GGCGGA 2 cut(s) 181, 528
Ecl136II GAGCTC 2 cut(s) 265, 415
Eco130I CCWWGG 1 cut(s) 134
Eco24I GRGCYC 2 cut(s) 267, 417
Eco31I GGTCTC 1 cut(s) 569
Eco47I GGWCC 2 cut(s) 28, 325
Eco53kI GAGCTC 2 cut(s) 265, 415
Eco88I CYCGRG 3 cut(s) 260, 321, 1351
Eco91I GGTNACC 1 cut(s) 337
EcoICRI GAGCTC 2 cut(s) 265, 415
EcoO65I GGTNACC 1 cut(s) 337
EcoRII CCWGG 1 cut(s) 1160
EcoT14I CCWWGG 1 cut(s) 134
EcoT22I ATGCAT 2 cut(s) 785, 1153
EcoT38I GRGCYC 2 cut(s) 267, 417
ErhI CCWWGG 1 cut(s) 134
FaeI CATG 6 cut(s) 138, 910, 1155, 1291, 1464, 1469
FatI CATG 6 cut(s) 134, 906, 1151, 1287, 1460, 1465
FauI CCCGC 1 cut(s) 401
FauNDI CATATG 1 cut(s) 1411
FbaI TGATCA 1 cut(s) 1468
FokI GGATG 2 cut(s) 53, 1386
FriOI GRGCYC 2 cut(s) 267, 417
FspBI CTAG 6 cut(s) 614, 1106, 1332, 1377, 1580, 1593
GlaI GCGC 3 cut(s) 12, 503, 792
GsaI CCCAGC 2 cut(s) 739, 1031
GsuI CTGGAG 1 cut(s) 1028
HaeII RGCGCY 1 cut(s) 14
HaeIII GGCC 3 cut(s) 1003, 1327, 1392
HapII CCGG 7 cut(s) 17, 288, 306, 314, 328, 381, 600
HgaI GACGC 2 cut(s) 29, 524
HhaI GCGC 3 cut(s) 13, 504, 793
Hin1II CATG 6 cut(s) 138, 910, 1155, 1291, 1464, 1469
Hin6I GCGC 3 cut(s) 11, 502, 791
HinP1I GCGC 3 cut(s) 11, 502, 791
HinfI GANTC 2 cut(s) 745, 1109
HpaII CCGG 7 cut(s) 17, 288, 306, 314, 328, 381, 600
HphI GGTGA 6 cut(s) 331, 647, 740, 977, 1043, 1489
Hpy188I TCNGA 3 cut(s) 298, 1123, 1473
Hpy188III TCNNGA 3 cut(s) 556, 896, 1007
Hpy99I CGWCG 1 cut(s) 942
HpyAV CCTTC 7 cut(s) 166, 175, 650, 672, 712, 1277, 1517
HpyCH4III ACNGT 3 cut(s) 343, 1086, 1317
HpyF10VI GCNNNNNNNGC 9 cut(s) 10, 250, 362, 371, 1009, 1157, 1166, 1558, 1567
HpyF3I CTNAG 6 cut(s) 43, 177, 619, 743, 831, 879
Hsp92II CATG 6 cut(s) 138, 910, 1155, 1291, 1464, 1469
HspAI GCGC 3 cut(s) 11, 502, 791
Ksp22I TGATCA 1 cut(s) 1468
Kzo9I GATC 7 cut(s) 584, 803, 898, 1223, 1245, 1257, 1468
LmnI GCTCC 5 cut(s) 186, 299, 412, 691, 1009
Lsp1109I GCAGC 8 cut(s) 352, 796, 824, 857, 931, 1024, 1156, 1225
LweI GCATC 3 cut(s) 770, 844, 1028
MaeI CTAG 6 cut(s) 614, 1106, 1332, 1377, 1580, 1593
MaeIII GTNAC 5 cut(s) 337, 667, 746, 1080, 1542
MalI GATC 7 cut(s) 586, 805, 900, 1225, 1247, 1259, 1470
MbiI CCGCTC 1 cut(s) 98
MboI GATC 7 cut(s) 584, 803, 898, 1223, 1245, 1257, 1468
MboII GAAGA 6 cut(s) 133, 178, 830, 866, 968, 1309
MfeI CAATTG 1 cut(s) 672
MhlI GDGCHC 3 cut(s) 267, 417, 863
MluCI AATT 4 cut(s) 150, 672, 868, 1565
MlyI GAGTC 1 cut(s) 754
MmeI TCCRAC 1 cut(s) 851
Mph1103I ATGCAT 2 cut(s) 785, 1153
MseI TTAA 4 cut(s) 590, 768, 1053, 1431
MspA1I CMGCKG 2 cut(s) 809, 1169
MspI CCGG 7 cut(s) 17, 288, 306, 314, 328, 381, 600
MspR9I CCNGG 6 cut(s) 288, 307, 315, 329, 382, 1162
MunI CAATTG 1 cut(s) 672
MvaI CCWGG 1 cut(s) 1162
MvnI CGCG 1 cut(s) 23
MwoI GCNNNNNNNGC 9 cut(s) 10, 250, 362, 371, 1009, 1157, 1166, 1558, 1567
NciI CCSGG 5 cut(s) 288, 307, 315, 329, 382
NcoI CCATGG 1 cut(s) 134
NdeI CATATG 1 cut(s) 1411
NdeII GATC 7 cut(s) 584, 803, 898, 1223, 1245, 1257, 1468
NheI GCTAGC 1 cut(s) 1592
NlaIII CATG 6 cut(s) 138, 910, 1155, 1291, 1464, 1469
NlaIV GGNNCC 4 cut(s) 50, 295, 326, 1326
NmuCI GTSAC 3 cut(s) 337, 746, 1542
NsiI ATGCAT 2 cut(s) 785, 1153
NspI RCATGY 1 cut(s) 1464
NspV TTCGAA 1 cut(s) 954
PaeI GCATGC 1 cut(s) 1464
PaeR7I CTCGAG 2 cut(s) 260, 1351
PaqCI CACCTGC 1 cut(s) 495
PfeI GAWTC 1 cut(s) 1109
PflFI GACNNNGTC 1 cut(s) 307
PleI GAGTC 1 cut(s) 753
PpsI GAGTC 1 cut(s) 753
Psp124BI GAGCTC 2 cut(s) 267, 417
Psp6I CCWGG 1 cut(s) 1160
PspEI GGTNACC 1 cut(s) 337
PspFI CCCAGC 2 cut(s) 735, 1027
PspGI CCWGG 1 cut(s) 1160
PspN4I GGNNCC 4 cut(s) 50, 295, 326, 1326
PspPI GGNCC 4 cut(s) 28, 325, 1325, 1391
PspXI VCTCGAGB 2 cut(s) 260, 1351
PstI CTGCAG 1 cut(s) 814
PstNI CAGNNNCTG 1 cut(s) 1084
PsyI GACNNNGTC 1 cut(s) 307
PvuII CAGCTG 2 cut(s) 809, 1169
SacI GAGCTC 2 cut(s) 267, 417
SaqAI TTAA 4 cut(s) 590, 768, 1053, 1431
Sau3AI GATC 7 cut(s) 584, 803, 898, 1223, 1245, 1257, 1468
Sau96I GGNCC 4 cut(s) 28, 325, 1325, 1391
SchI GAGTC 1 cut(s) 754
ScrFI CCNGG 6 cut(s) 288, 307, 315, 329, 382, 1162
SduI GDGCHC 3 cut(s) 267, 417, 863
SfaNI GCATC 3 cut(s) 770, 844, 1028
SfcI CTRYAG 1 cut(s) 810
Sfr274I CTCGAG 2 cut(s) 260, 1351
SfuI TTCGAA 1 cut(s) 954
SinI GGWCC 2 cut(s) 28, 325
SlaI CTCGAG 2 cut(s) 260, 1351
SmlI CTYRAG 4 cut(s) 248, 260, 1351, 1502
SmoI CTYRAG 4 cut(s) 248, 260, 1351, 1502
SphI GCATGC 1 cut(s) 1464
Sse9I AATT 4 cut(s) 150, 672, 868, 1565
SspMI CTAG 6 cut(s) 614, 1106, 1332, 1377, 1580, 1593
SstI GAGCTC 2 cut(s) 267, 417
StyD4I CCNGG 6 cut(s) 286, 305, 313, 327, 380, 1160
StyI CCWWGG 1 cut(s) 134
TaaI ACNGT 3 cut(s) 343, 1086, 1317
TaqI TCGA 4 cut(s) 261, 954, 1352, 1588
TaqII GACCGA 1 cut(s) 391
TasI AATT 4 cut(s) 150, 672, 868, 1565
TauI GCSGC 7 cut(s) 16, 294, 371, 374, 395, 796, 1003
TfiI GAWTC 1 cut(s) 1109
Tru1I TTAA 4 cut(s) 590, 768, 1053, 1431
Tru9I TTAA 4 cut(s) 590, 768, 1053, 1431
TscAI CASTG 3 cut(s) 1182, 1260, 1489
TseFI GTSAC 3 cut(s) 337, 746, 1542
TseI GCWGC 8 cut(s) 365, 809, 812, 845, 944, 1012, 1169, 1238
Tsp45I GTSAC 3 cut(s) 337, 746, 1542
TspGWI ACGGA 1 cut(s) 704
TspRI CASTG 3 cut(s) 1182, 1260, 1489
Tth111I GACNNNGTC 1 cut(s) 307
VpaK11BI GGWCC 2 cut(s) 28, 325
XceI RCATGY 1 cut(s) 1464
XcmI CCANNNNNNNNNTGG 1 cut(s) 132
XhoI CTCGAG 2 cut(s) 260, 1351
XspI CTAG 6 cut(s) 614, 1106, 1332, 1377, 1580, 1593
Zsp2I ATGCAT 2 cut(s) 785, 1153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.