pycom05g28520

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0019320
Physical Location & Seq
Reverse (-)
29201319 .. 29201891
573 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g28520.1

Sequence Viewer

Length: 573 bp
ATGGATGTTAACATTACAATCGTTCAGACTCGATTCTTACCTACATTTCCTACCCTAAACCAAAAAGCGACAACCCCACAAAATAACGTGAACATTTCAACAAACACCTTACCTACGAAAACCATACTCCTTTTTATCTGTACTAAACCCCAATGGAATCTAAGCCCTCTCACCCCTGATGCGCCTAGCAAGCTGGATATCCTTGGGCATGATGGTGACCCTCTTGGCGTGGATGGCGCAAAGGTTGGTGTCCTCAAAAAGCCCGACGAGGTAGGCCTCCGCCGCCTCCTGGAGGGCCGCAACGGCGGAGCTCTGGAACCTCAGGTCGGTCTTGAAGTCCTGGGCGATCTCACGGACCAGGCGCTGGAATGGGAGCTTTCGGATCAGGAGCTCAGTGCTCTTCTGGTACTTACGAATCTCACGGAGAGCGACGGTTCCGGGCCTGAAGCGGTGGGGCTTCTTCACTCCGCCGGTGGCCGGAGCAGACTTGCGAGCCGCCTTGGTGGCCAGCTGCTTGCGGGGAGCTTTTCCTCCGGTGGACTTGCGGGCGGTTTGCTTGGTGCGAGCCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

191

Amino Acids

19.84

Weight (kDa)

4.97

Isoelectric Point (pI)

36.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019320)

Species Orthologous Gene IDs
pyrus_communis pycom05g28520 pycom11g02110
rosa_laevigata RLG00000036715
rosa_roxburghii Rroxscaffold_1G00004320 Rroxscaffold_1G00066650 Rroxscaffold_1G00067350 Rroxscaffold_6G00389990
rosa_samantha Rh5AG074500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 364
AclWI GGATC 1 cut(s) 390
AcoI YGGCCR 2 cut(s) 475, 505
AcuI CTGAAG 1 cut(s) 465
AfaI GTAC 2 cut(s) 142, 408
AfiI CCNNNNNNNGG 5 cut(s) 289, 292, 326, 364, 477
AgsI TTSAA 2 cut(s) 99, 335
AjnI CCWGG 3 cut(s) 288, 339, 357
AluBI AGCT 6 cut(s) 193, 311, 376, 391, 511, 525
AluI AGCT 6 cut(s) 193, 311, 376, 391, 511, 525
Alw21I GWGCWC 3 cut(s) 313, 393, 400
AlwI GGATC 1 cut(s) 390
AlwNI CAGNNNCTG 1 cut(s) 364
AoxI GGCC 5 cut(s) 274, 295, 440, 475, 505
ApeKI GCWGC 1 cut(s) 511
AspLEI GCGC 3 cut(s) 184, 239, 364
AspS9I GGNCC 3 cut(s) 295, 355, 440
AsuC2I CCSGG 1 cut(s) 439
AsuHPI GGTGA 2 cut(s) 163, 227
AvaII GGWCC 1 cut(s) 355
AxyI CCTNAGG 1 cut(s) 321
BalI TGGCCA 1 cut(s) 507
BanII GRGCYC 2 cut(s) 313, 393
Bbv12I GWGCWC 3 cut(s) 313, 393, 400
BbvI GCAGC 1 cut(s) 498
BccI CCATC 2 cut(s) 206, 227
BceAI ACGGC 1 cut(s) 319
BciT130I CCWGG 3 cut(s) 290, 341, 359
BcnI CCSGG 1 cut(s) 439
BfaI CTAG 1 cut(s) 186
BfoI RGCGCY 1 cut(s) 365
BglI GCCNNNNNGGC 2 cut(s) 303, 504
BisI GCNGC 4 cut(s) 283, 298, 496, 512
BlsI GCNGC 4 cut(s) 284, 299, 497, 513
Bme1390I CCNGG 4 cut(s) 290, 341, 359, 439
Bme18I GGWCC 1 cut(s) 355
BmgT120I GGNCC 3 cut(s) 295, 355, 440
BmiI GGNNCC 2 cut(s) 318, 436
BmrFI CCNGG 4 cut(s) 290, 341, 359, 439
BmsI GCATC 1 cut(s) 169
BpmI CTGGAG 1 cut(s) 311
BpuMI CCSGG 1 cut(s) 439
BsaJI CCNNGG 3 cut(s) 202, 340, 499
BsaWI WCCGGW 1 cut(s) 533
Bsc4I CCNNNNNNNGG 5 cut(s) 289, 292, 326, 364, 477
Bse118I RCCGGY 1 cut(s) 470
Bse21I CCTNAGG 1 cut(s) 321
BseBI CCWGG 3 cut(s) 290, 341, 359
BseDI CCNNGG 3 cut(s) 202, 340, 499
BseGI GGATG 2 cut(s) 10, 238
BseLI CCNNNNNNNGG 5 cut(s) 289, 292, 326, 364, 477
BseMII CTCAG 2 cut(s) 335, 406
BseXI GCAGC 1 cut(s) 498
BshFI GGCC 5 cut(s) 276, 297, 442, 477, 507
BsiHKAI GWGCWC 3 cut(s) 313, 393, 400
BsiSI CCGG 4 cut(s) 438, 471, 478, 534
BslI CCNNNNNNNGG 5 cut(s) 289, 292, 326, 364, 477
BsnI GGCC 5 cut(s) 276, 297, 442, 477, 507
Bsp1286I GDGCHC 3 cut(s) 313, 393, 400
Bsp143I GATC 2 cut(s) 346, 382
BspANI GGCC 5 cut(s) 276, 297, 442, 477, 507
BspCNI CTCAG 2 cut(s) 334, 405
BspLI GGNNCC 2 cut(s) 318, 436
BspPI GGATC 1 cut(s) 390
BspQI GCTCTTC 1 cut(s) 405
BsrFI RCCGGY 1 cut(s) 470
BssAI RCCGGY 1 cut(s) 470
BssECI CCNNGG 3 cut(s) 202, 340, 499
BssMI GATC 2 cut(s) 346, 382
BssT1I CCWWGG 2 cut(s) 202, 499
Bst2UI CCWGG 3 cut(s) 290, 341, 359
Bst4CI ACNGT 1 cut(s) 434
Bst6I CTCTTC 1 cut(s) 405
BstC8I GCNNGC 6 cut(s) 191, 493, 509, 516, 547, 565
BstDEI CTNAG 3 cut(s) 161, 321, 392
BstEII GGTNACC 1 cut(s) 215
BstENI CCTNNNNNAGG 1 cut(s) 290
BstF5I GGATG 2 cut(s) 10, 238
BstH2I RGCGCY 1 cut(s) 365
BstHHI GCGC 3 cut(s) 184, 239, 364
BstKTI GATC 2 cut(s) 349, 385
BstMBI GATC 2 cut(s) 346, 382
BstMWI GCNNNNNNNGC 5 cut(s) 190, 234, 282, 303, 504
BstNI CCWGG 3 cut(s) 290, 341, 359
BstPI GGTNACC 1 cut(s) 215
BstSCI CCNGG 4 cut(s) 288, 339, 357, 437
BstV1I GCAGC 1 cut(s) 498
Bsu36I CCTNAGG 1 cut(s) 321
BsuRI GGCC 5 cut(s) 276, 297, 442, 477, 507
BtsCI GGATG 2 cut(s) 10, 238
BtsIMutI CAGTG 1 cut(s) 400
Cac8I GCNNGC 6 cut(s) 191, 493, 509, 516, 547, 565
CaiI CAGNNNCTG 1 cut(s) 364
CfoI GCGC 3 cut(s) 184, 239, 364
Cfr10I RCCGGY 1 cut(s) 470
Cfr13I GGNCC 3 cut(s) 295, 355, 440
Csp6I GTAC 2 cut(s) 141, 407
CviAII CATG 1 cut(s) 209
CviQI GTAC 2 cut(s) 141, 407
DdeI CTNAG 3 cut(s) 161, 321, 392
DpnI GATC 2 cut(s) 348, 384
DpnII GATC 2 cut(s) 346, 382
EaeI YGGCCR 2 cut(s) 475, 505
Eam1104I CTCTTC 1 cut(s) 405
EarI CTCTTC 1 cut(s) 405
EciI GGCGGA 3 cut(s) 269, 321, 457
Ecl136II GAGCTC 2 cut(s) 311, 391
Eco130I CCWWGG 2 cut(s) 202, 499
Eco147I AGGCCT 1 cut(s) 276
Eco24I GRGCYC 2 cut(s) 313, 393
Eco32I GATATC 1 cut(s) 199
Eco47I GGWCC 1 cut(s) 355
Eco53kI GAGCTC 2 cut(s) 311, 391
Eco57I CTGAAG 1 cut(s) 465
Eco81I CCTNAGG 1 cut(s) 321
Eco91I GGTNACC 1 cut(s) 215
EcoICRI GAGCTC 2 cut(s) 311, 391
EcoNI CCTNNNNNAGG 1 cut(s) 290
EcoO65I GGTNACC 1 cut(s) 215
EcoRII CCWGG 3 cut(s) 288, 339, 357
EcoRV GATATC 1 cut(s) 199
EcoT14I CCWWGG 2 cut(s) 202, 499
EcoT38I GRGCYC 2 cut(s) 313, 393
ErhI CCWWGG 2 cut(s) 202, 499
FaeI CATG 1 cut(s) 212
FaiI YATR 2 cut(s) 125, 210
FatI CATG 1 cut(s) 208
FauI CCCGC 2 cut(s) 511, 538
Fnu4HI GCNGC 4 cut(s) 283, 298, 496, 512
FokI GGATG 2 cut(s) 17, 245
FriOI GRGCYC 2 cut(s) 313, 393
Fsp4HI GCNGC 4 cut(s) 283, 298, 496, 512
FspBI CTAG 1 cut(s) 186
GlaI GCGC 3 cut(s) 183, 238, 363
GluI GCNGC 4 cut(s) 283, 298, 496, 512
GsuI CTGGAG 1 cut(s) 311
HaeII RGCGCY 1 cut(s) 365
HaeIII GGCC 5 cut(s) 276, 297, 442, 477, 507
HapII CCGG 4 cut(s) 438, 471, 478, 534
HhaI GCGC 3 cut(s) 184, 239, 364
Hin1II CATG 1 cut(s) 212
Hin6I GCGC 3 cut(s) 182, 237, 362
HinP1I GCGC 3 cut(s) 182, 237, 362
HincII GTYRAC 1 cut(s) 10
HindII GTYRAC 1 cut(s) 10
HinfI GANTC 4 cut(s) 28, 33, 157, 415
HpaI GTTAAC 1 cut(s) 10
HpaII CCGG 4 cut(s) 438, 471, 478, 534
HphI GGTGA 2 cut(s) 163, 227
Hpy166II GTNNAC 3 cut(s) 10, 91, 539
Hpy188I TCNGA 2 cut(s) 27, 382
Hpy188III TCNNGA 3 cut(s) 314, 332, 386
Hpy8I GTNNAC 3 cut(s) 10, 91, 539
Hpy99I CGWCG 2 cut(s) 269, 434
HpyCH4III ACNGT 1 cut(s) 434
HpyCH4IV ACGT 1 cut(s) 87
HpyF10VI GCNNNNNNNGC 5 cut(s) 190, 234, 282, 303, 504
HpyF3I CTNAG 3 cut(s) 161, 321, 392
HpySE526I ACGT 1 cut(s) 87
Hsp92II CATG 1 cut(s) 212
HspAI GCGC 3 cut(s) 182, 237, 362
KspAI GTTAAC 1 cut(s) 10
Kzo9I GATC 2 cut(s) 346, 382
LguI GCTCTTC 1 cut(s) 405
LmnI GCTCC 5 cut(s) 308, 373, 388, 480, 522
Lsp1109I GCAGC 1 cut(s) 498
LweI GCATC 1 cut(s) 169
MaeI CTAG 1 cut(s) 186
MaeII ACGT 1 cut(s) 87
MaeIII GTNAC 1 cut(s) 215
MalI GATC 2 cut(s) 348, 384
MboI GATC 2 cut(s) 346, 382
MboII GAAGA 2 cut(s) 392, 452
MhlI GDGCHC 3 cut(s) 313, 393, 400
MlsI TGGCCA 1 cut(s) 507
MluNI TGGCCA 1 cut(s) 507
MlyI GAGTC 1 cut(s) 22
MnlI CCTC 9 cut(s) 177, 231, 262, 263, 286, 287, 296, 330, 541
Mox20I TGGCCA 1 cut(s) 507
MscI TGGCCA 1 cut(s) 507
MseI TTAA 1 cut(s) 9
MslI CAYNNNNRTG 1 cut(s) 213
Msp20I TGGCCA 1 cut(s) 507
MspA1I CMGCKG 1 cut(s) 511
MspI CCGG 4 cut(s) 438, 471, 478, 534
MspR9I CCNGG 4 cut(s) 290, 341, 359, 439
MvaI CCWGG 3 cut(s) 290, 341, 359
MwoI GCNNNNNNNGC 5 cut(s) 190, 234, 282, 303, 504
NciI CCSGG 1 cut(s) 439
NdeII GATC 2 cut(s) 346, 382
NlaIII CATG 1 cut(s) 212
NlaIV GGNNCC 2 cut(s) 318, 436
NmuCI GTSAC 1 cut(s) 215
PceI AGGCCT 1 cut(s) 276
PciSI GCTCTTC 1 cut(s) 405
PfeI GAWTC 3 cut(s) 33, 157, 415
PflMI CCANNNNNTGG 1 cut(s) 364
PfoI TCCNGGA 1 cut(s) 288
PkrI GCNGC 4 cut(s) 284, 299, 497, 513
PleI GAGTC 1 cut(s) 22
PpsI GAGTC 1 cut(s) 22
Psp124BI GAGCTC 2 cut(s) 313, 393
Psp6I CCWGG 3 cut(s) 288, 339, 357
PspEI GGTNACC 1 cut(s) 215
PspGI CCWGG 3 cut(s) 288, 339, 357
PspN4I GGNNCC 2 cut(s) 318, 436
PspPI GGNCC 3 cut(s) 295, 355, 440
PstNI CAGNNNCTG 1 cut(s) 364
PvuII CAGCTG 1 cut(s) 511
RsaI GTAC 2 cut(s) 142, 408
RsaNI GTAC 2 cut(s) 141, 407
RseI CAYNNNNRTG 1 cut(s) 213
SacI GAGCTC 2 cut(s) 313, 393
SapI GCTCTTC 1 cut(s) 405
SaqAI TTAA 1 cut(s) 9
SatI GCNGC 4 cut(s) 283, 298, 496, 512
Sau3AI GATC 2 cut(s) 346, 382
Sau96I GGNCC 3 cut(s) 295, 355, 440
SchI GAGTC 1 cut(s) 22
ScrFI CCNGG 4 cut(s) 290, 341, 359, 439
SduI GDGCHC 3 cut(s) 313, 393, 400
SfaNI GCATC 1 cut(s) 169
SgrAI CRCCGGYG 1 cut(s) 470
SinI GGWCC 1 cut(s) 355
SmiMI CAYNNNNRTG 1 cut(s) 213
SseBI AGGCCT 1 cut(s) 276
SspMI CTAG 1 cut(s) 186
SstI GAGCTC 2 cut(s) 313, 393
StuI AGGCCT 1 cut(s) 276
StyD4I CCNGG 4 cut(s) 288, 339, 357, 437
StyI CCWWGG 2 cut(s) 202, 499
TaaI ACNGT 1 cut(s) 434
TaiI ACGT 1 cut(s) 90
TaqI TCGA 1 cut(s) 31
TaqII GACCGA 1 cut(s) 317
TatI WGTACW 1 cut(s) 140
TauI GCSGC 3 cut(s) 285, 300, 498
TfiI GAWTC 3 cut(s) 33, 157, 415
Tru1I TTAA 1 cut(s) 9
Tru9I TTAA 1 cut(s) 9
TscAI CASTG 1 cut(s) 400
TseFI GTSAC 1 cut(s) 215
TseI GCWGC 1 cut(s) 511
Tsp45I GTSAC 1 cut(s) 215
TspGWI ACGGA 2 cut(s) 368, 437
TspRI CASTG 1 cut(s) 400
Van91I CCANNNNNTGG 1 cut(s) 364
VpaK11BI GGWCC 1 cut(s) 355
XagI CCTNNNNNAGG 1 cut(s) 290
XspI CTAG 1 cut(s) 186
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.