Rroxscaffold_6G00389990

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0019320
Physical Location & Seq
Reverse (-)
4300359 .. 4301376
1018 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00389990.1

Sequence Viewer

Length: 444 bp
ATGAATGGCTTAAGCCCTCTCACCCCTGATTCTCCTAGCAAGCTGGATATCCTTAGGCATAATTGTAACACGCTTGGCATGAATGGCGCAGAGATTGGTATCCTCAAAGAGCCCAACAAGGTAGGCCTCCGCCGCCTCCTGCAGCGCCAACACGGCGTGGCTCTGGAAACGCAGGTCCGTCTTGAAGTCCTGCGCTATTTCCCGAACAAGCCTCTGGAACGGAAGCTTCCTTATCAGCAGCTCCGTGCTCTTCTGGTACTTTCGGATTTCACGCAGAGCCACCGTTCCAGGCCGGTACCTGTGCGGCTTCTTCACTCCTCCGGTCGTCGGGGCCGACTTCCGGGCAGCCTTCGTCGCCAGTTGCTTCCGAGGAGCCTTGCCGCCGGTCGACTTCCGAGCAGTCTGCTTTGTACGGGCCATCGACGAAATTCGAGATCAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

147

Amino Acids

16.63

Weight (kDa)

11.85

Isoelectric Point (pI)

80.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019320)

Species Orthologous Gene IDs
pyrus_communis pycom05g28520 pycom11g02110
rosa_laevigata RLG00000036715
rosa_roxburghii Rroxscaffold_1G00004320 Rroxscaffold_1G00066650 Rroxscaffold_1G00067350 Rroxscaffold_6G00389990
rosa_samantha Rh5AG074500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 163
Acc65I GGTACC 1 cut(s) 295
AccB1I GGYRCC 1 cut(s) 295
AccI GTMKAC 1 cut(s) 388
AciI CCGC 4 cut(s) 130, 133, 304, 381
AcsI RAATTY 1 cut(s) 427
AdeI CACNNNGTG 1 cut(s) 157
AfaI GTAC 3 cut(s) 258, 297, 412
AfiI CCNNNNNNNGG 2 cut(s) 327, 340
AflII CTTAAG 1 cut(s) 10
AgsI TTSAA 1 cut(s) 185
AjnI CCWGG 1 cut(s) 287
AluBI AGCT 3 cut(s) 43, 226, 241
AluI AGCT 3 cut(s) 43, 226, 241
Alw21I GWGCWC 1 cut(s) 250
AoxI GGCC 4 cut(s) 124, 290, 331, 415
ApeKI GCWGC 3 cut(s) 142, 238, 345
ApoI RAATTY 1 cut(s) 427
Asp718I GGTACC 1 cut(s) 295
AspLEI GCGC 3 cut(s) 89, 147, 195
AspS9I GGNCC 3 cut(s) 175, 331, 415
AsuC2I CCSGG 1 cut(s) 342
AsuHPI GGTGA 1 cut(s) 13
AvaII GGWCC 1 cut(s) 175
AxyI CCTNAGG 1 cut(s) 53
BanI GGYRCC 1 cut(s) 295
BanII GRGCYC 1 cut(s) 114
Bbv12I GWGCWC 1 cut(s) 250
BbvI GCAGC 3 cut(s) 154, 250, 357
BccI CCATC 1 cut(s) 426
BceAI ACGGC 1 cut(s) 169
BcgI CGANNNNNNTGC 2 cut(s) 385, 419
BciT130I CCWGG 1 cut(s) 289
BciVI GTATCC 1 cut(s) 110
BcnI CCSGG 1 cut(s) 342
BfaI CTAG 1 cut(s) 36
BfmI CTRYAG 1 cut(s) 140
BfoI RGCGCY 1 cut(s) 148
BfrI CTTAAG 1 cut(s) 10
BfuAI ACCTGC 1 cut(s) 163
BfuI GTATCC 1 cut(s) 110
BglI GCCNNNNNGGC 1 cut(s) 153
BisI GCNGC 6 cut(s) 133, 143, 239, 305, 346, 381
BlsI GCNGC 6 cut(s) 134, 144, 240, 306, 347, 382
Bme1390I CCNGG 2 cut(s) 289, 342
Bme18I GGWCC 1 cut(s) 175
BmgT120I GGNCC 3 cut(s) 175, 331, 415
BmiI GGNNCC 3 cut(s) 297, 332, 374
BmrFI CCNGG 2 cut(s) 289, 342
BpuMI CCSGG 1 cut(s) 342
BsaBI GATNNNNATC 1 cut(s) 98
BsaJI CCNNGG 1 cut(s) 368
BsaWI WCCGGW 1 cut(s) 320
Bsc4I CCNNNNNNNGG 2 cut(s) 327, 340
Bse118I RCCGGY 2 cut(s) 292, 383
Bse1I ACTGG 1 cut(s) 358
Bse21I CCTNAGG 1 cut(s) 53
Bse8I GATNNNNATC 1 cut(s) 98
BseBI CCWGG 1 cut(s) 289
BseDI CCNNGG 1 cut(s) 368
BseJI GATNNNNATC 1 cut(s) 98
BseLI CCNNNNNNNGG 2 cut(s) 327, 340
BseNI ACTGG 1 cut(s) 358
BseRI GAGGAG 2 cut(s) 307, 385
BseXI GCAGC 3 cut(s) 154, 250, 357
Bsh1285I CGRYCG 2 cut(s) 325, 388
BshFI GGCC 4 cut(s) 126, 292, 333, 417
BshNI GGYRCC 1 cut(s) 295
BsiEI CGRYCG 2 cut(s) 325, 388
BsiHKAI GWGCWC 1 cut(s) 250
BsiSI CCGG 4 cut(s) 293, 321, 341, 384
BslI CCNNNNNNNGG 2 cut(s) 327, 340
BsnI GGCC 4 cut(s) 126, 292, 333, 417
Bsp1286I GDGCHC 2 cut(s) 114, 250
Bsp143I GATC 1 cut(s) 434
BspACI CCGC 4 cut(s) 130, 133, 304, 381
BspANI GGCC 4 cut(s) 126, 292, 333, 417
BspLI GGNNCC 3 cut(s) 297, 332, 374
BspMAI CTGCAG 1 cut(s) 144
BspMI ACCTGC 1 cut(s) 163
BspQI GCTCTTC 1 cut(s) 255
BspT107I GGYRCC 1 cut(s) 295
BspTI CTTAAG 1 cut(s) 10
BsrFI RCCGGY 2 cut(s) 292, 383
BsrI ACTGG 1 cut(s) 358
BssAI RCCGGY 2 cut(s) 292, 383
BssECI CCNNGG 1 cut(s) 368
BssMI GATC 1 cut(s) 434
Bst2UI CCWGG 1 cut(s) 289
Bst4CI ACNGT 1 cut(s) 284
Bst6I CTCTTC 1 cut(s) 255
BstAFI CTTAAG 1 cut(s) 10
BstC8I GCNNGC 1 cut(s) 41
BstDEI CTNAG 1 cut(s) 53
BstH2I RGCGCY 1 cut(s) 148
BstHHI GCGC 3 cut(s) 89, 147, 195
BstKTI GATC 1 cut(s) 437
BstMBI GATC 1 cut(s) 434
BstMCI CGRYCG 2 cut(s) 325, 388
BstMWI GCNNNNNNNGC 4 cut(s) 84, 132, 153, 354
BstNI CCWGG 1 cut(s) 289
BstSCI CCNGG 2 cut(s) 287, 340
BstSFI CTRYAG 1 cut(s) 140
BstV1I GCAGC 3 cut(s) 154, 250, 357
Bsu36I CCTNAGG 1 cut(s) 53
BsuI GTATCC 1 cut(s) 110
BsuRI GGCC 4 cut(s) 126, 292, 333, 417
BveI ACCTGC 1 cut(s) 163
Cac8I GCNNGC 1 cut(s) 41
CfoI GCGC 3 cut(s) 89, 147, 195
Cfr10I RCCGGY 2 cut(s) 292, 383
Cfr13I GGNCC 3 cut(s) 175, 331, 415
Csp6I GTAC 3 cut(s) 257, 296, 411
CviAII CATG 1 cut(s) 79
CviQI GTAC 3 cut(s) 257, 296, 411
DdeI CTNAG 1 cut(s) 53
DpnI GATC 1 cut(s) 436
DpnII GATC 1 cut(s) 434
DraIII CACNNNGTG 1 cut(s) 157
Eam1104I CTCTTC 1 cut(s) 255
EarI CTCTTC 1 cut(s) 255
EciI GGCGGA 1 cut(s) 119
Eco147I AGGCCT 1 cut(s) 126
Eco24I GRGCYC 1 cut(s) 114
Eco32I GATATC 1 cut(s) 49
Eco47I GGWCC 1 cut(s) 175
Eco81I CCTNAGG 1 cut(s) 53
EcoRII CCWGG 1 cut(s) 287
EcoRV GATATC 1 cut(s) 49
EcoT38I GRGCYC 1 cut(s) 114
FaeI CATG 1 cut(s) 82
FaiI YATR 2 cut(s) 60, 80
FatI CATG 1 cut(s) 78
FblI GTMKAC 1 cut(s) 388
Fnu4HI GCNGC 6 cut(s) 133, 143, 239, 305, 346, 381
FriOI GRGCYC 1 cut(s) 114
Fsp4HI GCNGC 6 cut(s) 133, 143, 239, 305, 346, 381
FspBI CTAG 1 cut(s) 36
GlaI GCGC 3 cut(s) 88, 146, 194
GluI GCNGC 6 cut(s) 133, 143, 239, 305, 346, 381
HaeII RGCGCY 1 cut(s) 148
HaeIII GGCC 4 cut(s) 126, 292, 333, 417
HapII CCGG 4 cut(s) 293, 321, 341, 384
HhaI GCGC 3 cut(s) 89, 147, 195
Hin1II CATG 1 cut(s) 82
Hin6I GCGC 3 cut(s) 87, 145, 193
HinP1I GCGC 3 cut(s) 87, 145, 193
HincII GTYRAC 1 cut(s) 389
HindII GTYRAC 1 cut(s) 389
HindIII AAGCTT 1 cut(s) 224
HinfI GANTC 1 cut(s) 29
HpaII CCGG 4 cut(s) 293, 321, 341, 384
HphI GGTGA 1 cut(s) 13
Hpy166II GTNNAC 1 cut(s) 389
Hpy188I TCNGA 3 cut(s) 265, 369, 396
Hpy188III TCNNGA 6 cut(s) 164, 182, 202, 215, 432, 438
Hpy8I GTNNAC 1 cut(s) 389
Hpy99I CGWCG 3 cut(s) 330, 357, 426
HpyAV CCTTC 1 cut(s) 359
HpyCH4III ACNGT 1 cut(s) 284
HpyCH4V TGCA 1 cut(s) 142
HpyF10VI GCNNNNNNNGC 4 cut(s) 84, 132, 153, 354
HpyF3I CTNAG 1 cut(s) 53
Hsp92II CATG 1 cut(s) 82
HspAI GCGC 3 cut(s) 87, 145, 193
KpnI GGTACC 1 cut(s) 299
Kzo9I GATC 1 cut(s) 434
LguI GCTCTTC 1 cut(s) 255
LmnI GCTCC 2 cut(s) 246, 372
Lsp1109I GCAGC 3 cut(s) 154, 250, 357
MaeI CTAG 1 cut(s) 36
MaeIII GTNAC 1 cut(s) 65
MalI GATC 1 cut(s) 436
MboI GATC 1 cut(s) 434
MboII GAAGA 2 cut(s) 242, 302
MhlI GDGCHC 2 cut(s) 114, 250
MluCI AATT 2 cut(s) 61, 427
MnlI CCTC 7 cut(s) 27, 113, 137, 146, 222, 328, 363
MseI TTAA 1 cut(s) 11
MspCI CTTAAG 1 cut(s) 10
MspI CCGG 4 cut(s) 293, 321, 341, 384
MspR9I CCNGG 2 cut(s) 289, 342
MvaI CCWGG 1 cut(s) 289
MwoI GCNNNNNNNGC 4 cut(s) 84, 132, 153, 354
NciI CCSGG 1 cut(s) 342
NdeII GATC 1 cut(s) 434
NlaIII CATG 1 cut(s) 82
NlaIV GGNNCC 3 cut(s) 297, 332, 374
PceI AGGCCT 1 cut(s) 126
PciSI GCTCTTC 1 cut(s) 255
PcsI WCGNNNNNNNCGW 1 cut(s) 331
PfeI GAWTC 1 cut(s) 29
PkrI GCNGC 6 cut(s) 134, 144, 240, 306, 347, 382
Psp6I CCWGG 1 cut(s) 287
PspGI CCWGG 1 cut(s) 287
PspN4I GGNNCC 3 cut(s) 297, 332, 374
PspPI GGNCC 3 cut(s) 175, 331, 415
PstI CTGCAG 1 cut(s) 144
RsaI GTAC 3 cut(s) 258, 297, 412
RsaNI GTAC 3 cut(s) 257, 296, 411
SalI GTCGAC 1 cut(s) 387
SapI GCTCTTC 1 cut(s) 255
SaqAI TTAA 1 cut(s) 11
SatI GCNGC 6 cut(s) 133, 143, 239, 305, 346, 381
Sau3AI GATC 1 cut(s) 434
Sau96I GGNCC 3 cut(s) 175, 331, 415
ScrFI CCNGG 2 cut(s) 289, 342
SduI GDGCHC 2 cut(s) 114, 250
SetI ASST 6 cut(s) 45, 123, 177, 228, 243, 301
SfcI CTRYAG 1 cut(s) 140
SinI GGWCC 1 cut(s) 175
SmlI CTYRAG 1 cut(s) 10
SmoI CTYRAG 1 cut(s) 10
Sse9I AATT 2 cut(s) 61, 427
SseBI AGGCCT 1 cut(s) 126
SsiI CCGC 4 cut(s) 130, 133, 304, 381
SspMI CTAG 1 cut(s) 36
StuI AGGCCT 1 cut(s) 126
StyD4I CCNGG 2 cut(s) 287, 340
TaaI ACNGT 1 cut(s) 284
TaqI TCGA 3 cut(s) 388, 421, 431
TasI AATT 2 cut(s) 61, 427
TauI GCSGC 3 cut(s) 135, 307, 383
TfiI GAWTC 1 cut(s) 29
Tru1I TTAA 1 cut(s) 11
Tru9I TTAA 1 cut(s) 11
TseI GCWGC 3 cut(s) 142, 238, 345
TspDTI ATGAA 2 cut(s) 17, 95
TspGWI ACGGA 3 cut(s) 167, 233, 235
Vha464I CTTAAG 1 cut(s) 10
VpaK11BI GGWCC 1 cut(s) 175
XapI RAATTY 1 cut(s) 427
XmiI GTMKAC 1 cut(s) 388
XspI CTAG 1 cut(s) 36
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.