Rh5AG074500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0019320
Physical Location & Seq
Forward (+)
6308078 .. 6308747
670 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG074500.1

Sequence Viewer

Length: 408 bp
ATGAATGGCACACAGATTGGTGTCCTCAAAGAGCCCGACGAGGTAGGCCTCCGCCGCCTCCTGCAGCGCAGCCACGGCGGAGCTCTGGAACCTCAGATCGGTCTTGAAATCCTGAGCGATTTCACGGACCAACCGCTGGAATGGGAGCTTGCGGATCAGAAGCTCCGTGCTCTTCTGGTACTTGCGGATCTCACGGAGCGCGACGGTTCCCGGCCTGAATCTGTGTGGCTTCTTCACTCCGCCGGTGGCCGGAGCGGACTTCCTGGCGGCCTTGGTGGCAAGCTGCTTACGTGGCGCCTTGCCTCCGGTGGACTTGCGGGCGGTTTGCTTGGTACGAGCCATTGTGGGATTGGGAAATTAGGGTTCGAAAGATTTCTTGGGTTTTCAGAGGAATTGGGGATTTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

135

Amino Acids

14.44

Weight (kDa)

5.34

Isoelectric Point (pI)

38.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019320)

Species Orthologous Gene IDs
pyrus_communis pycom05g28520 pycom11g02110
rosa_laevigata RLG00000036715
rosa_roxburghii Rroxscaffold_1G00004320 Rroxscaffold_1G00066650 Rroxscaffold_1G00067350 Rroxscaffold_6G00389990
rosa_samantha Rh5AG074500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 294
AccB7I CCANNNNNTGG 1 cut(s) 136
AccBSI CCGCTC 1 cut(s) 255
AccII CGCG 1 cut(s) 201
AclWI GGATC 2 cut(s) 162, 195
AcoI YGGCCR 1 cut(s) 247
AcyI GRCGYC 1 cut(s) 295
AfaI GTAC 2 cut(s) 180, 334
AfiI CCNNNNNNNGG 3 cut(s) 98, 136, 249
AgsI TTSAA 1 cut(s) 107
AjnI CCWGG 1 cut(s) 262
AjuI GAANNNNNNNTTGG 2 cut(s) 360, 392
AluBI AGCT 4 cut(s) 83, 148, 163, 283
AluI AGCT 4 cut(s) 83, 148, 163, 283
Alw21I GWGCWC 2 cut(s) 85, 172
AlwI GGATC 2 cut(s) 162, 195
AoxI GGCC 4 cut(s) 46, 212, 247, 268
ApeKI GCWGC 3 cut(s) 64, 69, 283
Asp700I GAANNNNTTC 1 cut(s) 372
AspLEI GCGC 3 cut(s) 69, 201, 297
AspS9I GGNCC 1 cut(s) 127
AsuC2I CCSGG 1 cut(s) 211
AsuII TTCGAA 1 cut(s) 366
AvaII GGWCC 1 cut(s) 127
BanI GGYRCC 1 cut(s) 294
BanII GRGCYC 2 cut(s) 36, 85
Bbv12I GWGCWC 2 cut(s) 85, 172
BbvI GCAGC 3 cut(s) 76, 81, 270
BceAI ACGGC 1 cut(s) 91
BciT130I CCWGG 1 cut(s) 264
BcnI CCSGG 1 cut(s) 211
BfmI CTRYAG 1 cut(s) 62
BfoI RGCGCY 1 cut(s) 298
BglI GCCNNNNNGGC 1 cut(s) 276
BisI GCNGC 5 cut(s) 55, 65, 70, 268, 284
BlsI GCNGC 5 cut(s) 56, 66, 71, 269, 285
Bme1390I CCNGG 2 cut(s) 211, 264
Bme18I GGWCC 1 cut(s) 127
BmgT120I GGNCC 1 cut(s) 127
BmiI GGNNCC 3 cut(s) 90, 208, 296
BmrFI CCNGG 2 cut(s) 211, 264
Bpu10I CCTNAGC 1 cut(s) 113
Bpu14I TTCGAA 1 cut(s) 366
BpuMI CCSGG 1 cut(s) 211
BsaAI YACGTR 1 cut(s) 291
BsaHI GRCGYC 1 cut(s) 295
BsaJI CCNNGG 2 cut(s) 73, 271
BsaWI WCCGGW 1 cut(s) 305
Bsc4I CCNNNNNNNGG 3 cut(s) 98, 136, 249
Bse118I RCCGGY 1 cut(s) 242
BseBI CCWGG 1 cut(s) 264
BseDI CCNNGG 2 cut(s) 73, 271
BseLI CCNNNNNNNGG 3 cut(s) 98, 136, 249
BseMII CTCAG 2 cut(s) 104, 107
BseXI GCAGC 3 cut(s) 76, 81, 270
Bsh1236I CGCG 1 cut(s) 201
BshFI GGCC 4 cut(s) 48, 214, 249, 270
BshNI GGYRCC 1 cut(s) 294
BsiHKAI GWGCWC 2 cut(s) 85, 172
BsiSI CCGG 4 cut(s) 211, 243, 250, 306
BslI CCNNNNNNNGG 3 cut(s) 98, 136, 249
BsnI GGCC 4 cut(s) 48, 214, 249, 270
Bsp119I TTCGAA 1 cut(s) 366
Bsp1286I GDGCHC 3 cut(s) 36, 85, 172
Bsp143I GATC 3 cut(s) 96, 154, 187
BspANI GGCC 4 cut(s) 48, 214, 249, 270
BspCNI CTCAG 2 cut(s) 105, 106
BspFNI CGCG 1 cut(s) 201
BspLI GGNNCC 3 cut(s) 90, 208, 296
BspMAI CTGCAG 1 cut(s) 66
BspPI GGATC 2 cut(s) 162, 195
BspQI GCTCTTC 1 cut(s) 177
BspT104I TTCGAA 1 cut(s) 366
BspT107I GGYRCC 1 cut(s) 294
BsrBI CCGCTC 1 cut(s) 255
BsrFI RCCGGY 1 cut(s) 242
BssAI RCCGGY 1 cut(s) 242
BssECI CCNNGG 2 cut(s) 73, 271
BssMI GATC 3 cut(s) 96, 154, 187
BssNI GRCGYC 1 cut(s) 295
BssT1I CCWWGG 1 cut(s) 271
Bst2UI CCWGG 1 cut(s) 264
Bst4CI ACNGT 1 cut(s) 206
Bst6I CTCTTC 1 cut(s) 177
BstACI GRCGYC 1 cut(s) 295
BstBAI YACGTR 1 cut(s) 291
BstBI TTCGAA 1 cut(s) 366
BstC8I GCNNGC 3 cut(s) 150, 281, 319
BstDEI CTNAG 2 cut(s) 93, 113
BstDSI CCRYGG 1 cut(s) 73
BstFNI CGCG 1 cut(s) 201
BstH2I RGCGCY 1 cut(s) 298
BstHHI GCGC 3 cut(s) 69, 201, 297
BstKTI GATC 3 cut(s) 99, 157, 190
BstMBI GATC 3 cut(s) 96, 154, 187
BstMWI GCNNNNNNNGC 4 cut(s) 54, 75, 276, 292
BstNI CCWGG 1 cut(s) 264
BstSCI CCNGG 2 cut(s) 209, 262
BstSFI CTRYAG 1 cut(s) 62
BstUI CGCG 1 cut(s) 201
BstV1I GCAGC 3 cut(s) 76, 81, 270
BstX2I RGATCY 1 cut(s) 187
BstYI RGATCY 1 cut(s) 187
BsuRI GGCC 4 cut(s) 48, 214, 249, 270
BtgI CCRYGG 1 cut(s) 73
Cac8I GCNNGC 3 cut(s) 150, 281, 319
CfoI GCGC 3 cut(s) 69, 201, 297
Cfr10I RCCGGY 1 cut(s) 242
Cfr13I GGNCC 1 cut(s) 127
Csp6I GTAC 2 cut(s) 179, 333
CviQI GTAC 2 cut(s) 179, 333
DdeI CTNAG 2 cut(s) 93, 113
DinI GGCGCC 1 cut(s) 296
DpnI GATC 3 cut(s) 98, 156, 189
DpnII GATC 3 cut(s) 96, 154, 187
EaeI YGGCCR 1 cut(s) 247
Eam1104I CTCTTC 1 cut(s) 177
EarI CTCTTC 1 cut(s) 177
EciI GGCGGA 3 cut(s) 41, 93, 229
Ecl136II GAGCTC 1 cut(s) 83
Eco130I CCWWGG 1 cut(s) 271
Eco147I AGGCCT 1 cut(s) 48
Eco24I GRGCYC 2 cut(s) 36, 85
Eco47I GGWCC 1 cut(s) 127
Eco53kI GAGCTC 1 cut(s) 83
EcoICRI GAGCTC 1 cut(s) 83
EcoRII CCWGG 1 cut(s) 262
EcoT14I CCWWGG 1 cut(s) 271
EcoT38I GRGCYC 2 cut(s) 36, 85
EgeI GGCGCC 1 cut(s) 296
EheI GGCGCC 1 cut(s) 296
ErhI CCWWGG 1 cut(s) 271
FauI CCCGC 1 cut(s) 310
Fnu4HI GCNGC 5 cut(s) 55, 65, 70, 268, 284
FriOI GRGCYC 2 cut(s) 36, 85
Fsp4HI GCNGC 5 cut(s) 55, 65, 70, 268, 284
GlaI GCGC 3 cut(s) 68, 200, 296
GluI GCNGC 5 cut(s) 55, 65, 70, 268, 284
HaeII RGCGCY 1 cut(s) 298
HaeIII GGCC 4 cut(s) 48, 214, 249, 270
HapII CCGG 4 cut(s) 211, 243, 250, 306
HhaI GCGC 3 cut(s) 69, 201, 297
Hin1I GRCGYC 1 cut(s) 295
Hin6I GCGC 3 cut(s) 67, 199, 295
HinP1I GCGC 3 cut(s) 67, 199, 295
HinfI GANTC 1 cut(s) 218
HpaII CCGG 4 cut(s) 211, 243, 250, 306
Hpy166II GTNNAC 1 cut(s) 311
Hpy188I TCNGA 3 cut(s) 96, 159, 388
Hpy188III TCNNGA 3 cut(s) 86, 104, 112
Hpy8I GTNNAC 1 cut(s) 311
Hpy99I CGWCG 2 cut(s) 41, 206
HpyCH4III ACNGT 1 cut(s) 206
HpyCH4IV ACGT 1 cut(s) 290
HpyCH4V TGCA 1 cut(s) 64
HpyF10VI GCNNNNNNNGC 4 cut(s) 54, 75, 276, 292
HpyF3I CTNAG 2 cut(s) 93, 113
HpySE526I ACGT 1 cut(s) 290
Hsp92I GRCGYC 1 cut(s) 295
HspAI GCGC 3 cut(s) 67, 199, 295
KasI GGCGCC 1 cut(s) 294
Kzo9I GATC 3 cut(s) 96, 154, 187
LguI GCTCTTC 1 cut(s) 177
LmnI GCTCC 5 cut(s) 80, 145, 168, 196, 252
Lsp1109I GCAGC 3 cut(s) 76, 81, 270
MaeII ACGT 1 cut(s) 290
MalI GATC 3 cut(s) 98, 156, 189
MbiI CCGCTC 1 cut(s) 255
MboI GATC 3 cut(s) 96, 154, 187
MboII GAAGA 2 cut(s) 164, 224
MflI RGATCY 1 cut(s) 187
MhlI GDGCHC 3 cut(s) 36, 85, 172
MluCI AATT 2 cut(s) 356, 392
Mly113I GGCGCC 1 cut(s) 295
MnlI CCTC 7 cut(s) 34, 35, 59, 68, 102, 313, 382
MroXI GAANNNNTTC 1 cut(s) 372
MspA1I CMGCKG 1 cut(s) 136
MspI CCGG 4 cut(s) 211, 243, 250, 306
MspR9I CCNGG 2 cut(s) 211, 264
MvaI CCWGG 1 cut(s) 264
MvnI CGCG 1 cut(s) 201
MwoI GCNNNNNNNGC 4 cut(s) 54, 75, 276, 292
NarI GGCGCC 1 cut(s) 295
NciI CCSGG 1 cut(s) 211
NdeII GATC 3 cut(s) 96, 154, 187
NlaIV GGNNCC 3 cut(s) 90, 208, 296
NspV TTCGAA 1 cut(s) 366
PceI AGGCCT 1 cut(s) 48
PciSI GCTCTTC 1 cut(s) 177
PdmI GAANNNNTTC 1 cut(s) 372
PfeI GAWTC 1 cut(s) 218
PflMI CCANNNNNTGG 1 cut(s) 136
PkrI GCNGC 5 cut(s) 56, 66, 71, 269, 285
PluTI GGCGCC 1 cut(s) 298
Ppu21I YACGTR 1 cut(s) 291
Psp124BI GAGCTC 1 cut(s) 85
Psp6I CCWGG 1 cut(s) 262
PspGI CCWGG 1 cut(s) 262
PspN4I GGNNCC 3 cut(s) 90, 208, 296
PspPI GGNCC 1 cut(s) 127
PstI CTGCAG 1 cut(s) 66
PsuI RGATCY 1 cut(s) 187
RsaI GTAC 2 cut(s) 180, 334
RsaNI GTAC 2 cut(s) 179, 333
SacI GAGCTC 1 cut(s) 85
SapI GCTCTTC 1 cut(s) 177
SatI GCNGC 5 cut(s) 55, 65, 70, 268, 284
Sau3AI GATC 3 cut(s) 96, 154, 187
Sau96I GGNCC 1 cut(s) 127
ScrFI CCNGG 2 cut(s) 211, 264
SduI GDGCHC 3 cut(s) 36, 85, 172
SetI ASST 7 cut(s) 45, 85, 94, 150, 165, 285, 293
SfcI CTRYAG 1 cut(s) 62
SfoI GGCGCC 1 cut(s) 296
SfuI TTCGAA 1 cut(s) 366
SgrAI CRCCGGYG 1 cut(s) 242
SinI GGWCC 1 cut(s) 127
Sse9I AATT 2 cut(s) 356, 392
SseBI AGGCCT 1 cut(s) 48
SspDI GGCGCC 1 cut(s) 294
SstI GAGCTC 1 cut(s) 85
StuI AGGCCT 1 cut(s) 48
StyD4I CCNGG 2 cut(s) 209, 262
StyI CCWWGG 1 cut(s) 271
TaaI ACNGT 1 cut(s) 206
TaiI ACGT 1 cut(s) 293
TaqI TCGA 1 cut(s) 366
TaqII GACCGA 1 cut(s) 89
TasI AATT 2 cut(s) 356, 392
TauI GCSGC 2 cut(s) 57, 270
TfiI GAWTC 1 cut(s) 218
TseI GCWGC 3 cut(s) 64, 69, 283
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 3 cut(s) 140, 155, 209
Van91I CCANNNNNTGG 1 cut(s) 136
VpaK11BI GGWCC 1 cut(s) 127
XcmI CCANNNNNNNNNTGG 1 cut(s) 347
XmnI GAANNNNTTC 1 cut(s) 372
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.