pycom05g29480

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0014505
Physical Location & Seq
Reverse (-)
29881362 .. 29883404
2043 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 666 bp
ATGATTTTTCCAAACGCTTTTTTATCTTCTTTTTCGTGTCTGTCTGTCTGTCTCTGCGATTTGTGTTGGGGTTTTAGACGAAATTCGAGTTCGAGAGAAAGCGAGGGGTTTTGTGGGATGGCTTCAACACTGCTTTTGGTTGTCGTTTTTGTGTTCGATCTGGTTGCTTTTGCGCTGGCTGTTGCCGCTGAGCAGCGAAGGTCCACTGTAAGTGCCAAGGTTCAAACTGAAGCAAATTCGACCTATTGTGTATATGATTCTGACATTGCAACTGGCTTAGGTGTGGGATCATTTCTGTTCTTATTGGCAAGTCAAGCTCTGATAATGGTGGCAAGTCGATGCTTGTGCTGTGGAAAGGCTTTGAGACCCAGCGGTTCTCGATCGTGGGCAATTACGCTCTTCATTACCAGCTGGGTGCTTTTTGTCATTGCTGAGGCATGCCTTCTCGCGGGTTCAGTGAGAAATGCGTACCACACAAAGTACACAACTTCGTTTATGGACGACCTTTCATGTGAGACATTGAGGAAAGGGATCTTCGGAGCTGGGGCTGCATTTGTCGTCCTCACAGGCATATTCTCGGAGCTTTACTATGTTAGCTATTCCAGGGCTAACGAATCCCCGGCTCCCTATAGCAGAGACGCCGGTGTGAGGATGCAGAACCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

222

Amino Acids

23.98

Weight (kDa)

7.99

Isoelectric Point (pI)

48.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 97 - 190 2.4e-30 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014505)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G61065
fragaria_vesca FvH4_3g04720
prunus_persica Prupe.4G042400_v2.0.a1 Prupe.4G042400_v2.0.a1
pyrus_communis pycom05g29480
rosa_chinensis RchiOBHm_Chr5g0007741
rosa_laevigata RLG00000031483
rosa_multiflora Rmu_sc0005098.1_g000021
rosa_roxburghii Rroxscaffold_1G00068670
rosa_rugosa Rorug04G0429800 Rorug04G0429900 Rorug04G0430000
rosa_samantha Rh5AG058300 Rh5BG055100 Rh5CG064800 Rh5DG054100
rosa_wichuraiana Rw5G006140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 449
AciI CCGC 3 cut(s) 186, 372, 449
AclWI GGATC 2 cut(s) 295, 539
AcsI RAATTY 2 cut(s) 82, 235
AcuI CTGAAG 1 cut(s) 249
AcyI GRCGYC 1 cut(s) 639
AfaI GTAC 2 cut(s) 470, 482
AfiI CCNNNNNNNGG 2 cut(s) 448, 648
AgsI TTSAA 2 cut(s) 126, 224
AjnI CCWGG 1 cut(s) 602
AluBI AGCT 5 cut(s) 317, 411, 542, 583, 597
AluI AGCT 5 cut(s) 317, 411, 542, 583, 597
Alw26I GTCTC 4 cut(s) 56, 358, 509, 630
AlwI GGATC 2 cut(s) 295, 539
ApeKI GCWGC 2 cut(s) 193, 548
ApoI RAATTY 2 cut(s) 82, 235
AspLEI GCGC 1 cut(s) 175
AspS9I GGNCC 1 cut(s) 201
AsuC2I CCSGG 1 cut(s) 620
AvaII GGWCC 1 cut(s) 201
BbvCI CCTCAGC 1 cut(s) 432
BbvI GCAGC 2 cut(s) 205, 535
BccI CCATC 1 cut(s) 112
BcgI CGANNNNNNTGC 4 cut(s) 146, 180, 327, 361
BciT130I CCWGG 1 cut(s) 604
BcnI CCSGG 1 cut(s) 620
BcoDI GTCTC 4 cut(s) 56, 358, 509, 630
BfmI CTRYAG 1 cut(s) 628
BisI GCNGC 3 cut(s) 186, 194, 549
BlpI GCTNAGC 1 cut(s) 189
BlsI GCNGC 3 cut(s) 187, 195, 550
Bme1390I CCNGG 2 cut(s) 604, 620
Bme18I GGWCC 1 cut(s) 201
BmgT120I GGNCC 1 cut(s) 201
BmiI GGNNCC 1 cut(s) 624
BmrFI CCNGG 2 cut(s) 604, 620
BmsI GCATC 2 cut(s) 329, 642
Bpu10I CCTNAGC 2 cut(s) 277, 432
Bpu1102I GCTNAGC 1 cut(s) 189
BpuMI CCSGG 1 cut(s) 620
BsaHI GRCGYC 1 cut(s) 639
BsaI GGTCTC 1 cut(s) 358
BsaJI CCNNGG 3 cut(s) 216, 603, 618
Bsc4I CCNNNNNNNGG 2 cut(s) 448, 648
Bse118I RCCGGY 1 cut(s) 641
Bse1I ACTGG 1 cut(s) 277
Bse3DI GCAATG 2 cut(s) 264, 426
BseBI CCWGG 1 cut(s) 604
BseDI CCNNGG 3 cut(s) 216, 603, 618
BseGI GGATG 2 cut(s) 123, 657
BseLI CCNNNNNNNGG 2 cut(s) 448, 648
BseMI GCAATG 2 cut(s) 264, 426
BseMII CTCAG 2 cut(s) 180, 423
BseNI ACTGG 1 cut(s) 277
BseXI GCAGC 2 cut(s) 205, 535
BseYI CCCAGC 3 cut(s) 368, 411, 542
Bsh1236I CGCG 1 cut(s) 449
Bsh1285I CGRYCG 1 cut(s) 383
BsiEI CGRYCG 1 cut(s) 383
BsiSI CCGG 2 cut(s) 620, 642
BslI CCNNNNNNNGG 2 cut(s) 448, 648
BsmAI GTCTC 4 cut(s) 56, 358, 509, 630
BsmBI CGTCTC 1 cut(s) 630
Bso31I GGTCTC 1 cut(s) 358
Bsp143I GATC 4 cut(s) 157, 287, 380, 531
Bsp1720I GCTNAGC 1 cut(s) 189
BspACI CCGC 3 cut(s) 186, 372, 449
BspCNI CTCAG 2 cut(s) 181, 424
BspFNI CGCG 1 cut(s) 449
BspLI GGNNCC 1 cut(s) 624
BspPI GGATC 2 cut(s) 295, 539
BspQI GCTCTTC 1 cut(s) 404
BspTNI GGTCTC 1 cut(s) 358
BsrDI GCAATG 2 cut(s) 264, 426
BsrFI RCCGGY 1 cut(s) 641
BsrI ACTGG 1 cut(s) 277
BssAI RCCGGY 1 cut(s) 641
BssECI CCNNGG 3 cut(s) 216, 603, 618
BssMI GATC 4 cut(s) 157, 287, 380, 531
BssNI GRCGYC 1 cut(s) 639
BssT1I CCWWGG 1 cut(s) 216
Bst2UI CCWGG 1 cut(s) 604
Bst4CI ACNGT 1 cut(s) 208
Bst6I CTCTTC 1 cut(s) 404
BstACI GRCGYC 1 cut(s) 639
BstC8I GCNNGC 2 cut(s) 177, 439
BstDEI CTNAG 3 cut(s) 189, 277, 432
BstF5I GGATG 2 cut(s) 123, 657
BstFNI CGCG 1 cut(s) 449
BstHHI GCGC 1 cut(s) 175
BstKTI GATC 4 cut(s) 160, 290, 383, 534
BstMAI GTCTC 4 cut(s) 56, 358, 509, 630
BstMBI GATC 4 cut(s) 157, 287, 380, 531
BstMCI CGRYCG 1 cut(s) 383
BstMWI GCNNNNNNNGC 3 cut(s) 185, 314, 548
BstNI CCWGG 1 cut(s) 604
BstNSI RCATGY 1 cut(s) 441
BstSCI CCNGG 2 cut(s) 602, 618
BstSFI CTRYAG 1 cut(s) 628
BstUI CGCG 1 cut(s) 449
BstV1I GCAGC 2 cut(s) 205, 535
BstX2I RGATCY 1 cut(s) 531
BstYI RGATCY 1 cut(s) 531
BtsCI GGATG 2 cut(s) 123, 657
BtsI GCAGTG 1 cut(s) 128
BtsIMutI CAGTG 3 cut(s) 128, 204, 462
Cac8I GCNNGC 2 cut(s) 177, 439
CfoI GCGC 1 cut(s) 175
Cfr10I RCCGGY 1 cut(s) 641
Cfr13I GGNCC 1 cut(s) 201
CseI GACGC 1 cut(s) 647
Csp6I GTAC 2 cut(s) 469, 481
CviAII CATG 2 cut(s) 438, 510
CviQI GTAC 2 cut(s) 469, 481
DdeI CTNAG 3 cut(s) 189, 277, 432
DpnI GATC 4 cut(s) 159, 289, 382, 533
DpnII GATC 4 cut(s) 157, 287, 380, 531
Eam1104I CTCTTC 1 cut(s) 404
EarI CTCTTC 1 cut(s) 404
Eco130I CCWWGG 1 cut(s) 216
Eco31I GGTCTC 1 cut(s) 358
Eco47I GGWCC 1 cut(s) 201
Eco57I CTGAAG 1 cut(s) 249
EcoRII CCWGG 1 cut(s) 602
EcoT14I CCWWGG 1 cut(s) 216
ErhI CCWWGG 1 cut(s) 216
Esp3I CGTCTC 1 cut(s) 630
FaeI CATG 2 cut(s) 441, 513
FaiI YATR 8 cut(s) 253, 255, 439, 497, 511, 572, 591, 630
FatI CATG 2 cut(s) 437, 509
FauI CCCGC 1 cut(s) 442
Fnu4HI GCNGC 3 cut(s) 186, 194, 549
FokI GGATG 1 cut(s) 130
Fsp4HI GCNGC 3 cut(s) 186, 194, 549
GlaI GCGC 1 cut(s) 174
GluI GCNGC 3 cut(s) 186, 194, 549
GsaI CCCAGC 3 cut(s) 372, 415, 546
HapII CCGG 2 cut(s) 620, 642
HgaI GACGC 1 cut(s) 647
HhaI GCGC 1 cut(s) 175
Hin1I GRCGYC 1 cut(s) 639
Hin1II CATG 2 cut(s) 441, 513
Hin6I GCGC 1 cut(s) 173
HinP1I GCGC 1 cut(s) 173
HinfI GANTC 2 cut(s) 257, 614
HpaII CCGG 2 cut(s) 620, 642
Hpy166II GTNNAC 2 cut(s) 204, 483
Hpy188I TCNGA 4 cut(s) 262, 321, 539, 580
Hpy188III TCNNGA 2 cut(s) 93, 378
Hpy8I GTNNAC 2 cut(s) 204, 483
HpyAV CCTTC 2 cut(s) 192, 452
HpyCH4III ACNGT 1 cut(s) 208
HpyCH4V TGCA 3 cut(s) 269, 551, 655
HpyF10VI GCNNNNNNNGC 3 cut(s) 185, 314, 548
HpyF3I CTNAG 3 cut(s) 189, 277, 432
Hsp92I GRCGYC 1 cut(s) 639
Hsp92II CATG 2 cut(s) 441, 513
HspAI GCGC 1 cut(s) 173
Kzo9I GATC 4 cut(s) 157, 287, 380, 531
LguI GCTCTTC 1 cut(s) 404
LmnI GCTCC 3 cut(s) 539, 580, 628
Lsp1109I GCAGC 2 cut(s) 205, 535
LweI GCATC 2 cut(s) 329, 642
MalI GATC 4 cut(s) 159, 289, 382, 533
MboI GATC 4 cut(s) 157, 287, 380, 531
MboII GAAGA 3 cut(s) 18, 391, 526
MflI RGATCY 1 cut(s) 531
MluCI AATT 3 cut(s) 82, 235, 390
MnlI CCTC 5 cut(s) 97, 427, 516, 572, 642
MspA1I CMGCKG 3 cut(s) 188, 372, 411
MspI CCGG 2 cut(s) 620, 642
MspR9I CCNGG 2 cut(s) 604, 620
MvaI CCWGG 1 cut(s) 604
MvnI CGCG 1 cut(s) 449
MwoI GCNNNNNNNGC 3 cut(s) 185, 314, 548
NciI CCSGG 1 cut(s) 620
NdeII GATC 4 cut(s) 157, 287, 380, 531
NlaIII CATG 2 cut(s) 441, 513
NlaIV GGNNCC 1 cut(s) 624
NspI RCATGY 1 cut(s) 441
PaeI GCATGC 1 cut(s) 441
PciSI GCTCTTC 1 cut(s) 404
PfeI GAWTC 2 cut(s) 257, 614
PkrI GCNGC 3 cut(s) 187, 195, 550
Ple19I CGATCG 1 cut(s) 383
Psp6I CCWGG 1 cut(s) 602
PspFI CCCAGC 3 cut(s) 368, 411, 542
PspGI CCWGG 1 cut(s) 602
PspN4I GGNNCC 1 cut(s) 624
PspPI GGNCC 1 cut(s) 201
PsuI RGATCY 1 cut(s) 531
PvuI CGATCG 1 cut(s) 383
PvuII CAGCTG 1 cut(s) 411
RsaI GTAC 2 cut(s) 470, 482
RsaNI GTAC 2 cut(s) 469, 481
SapI GCTCTTC 1 cut(s) 404
SatI GCNGC 3 cut(s) 186, 194, 549
Sau3AI GATC 4 cut(s) 157, 287, 380, 531
Sau96I GGNCC 1 cut(s) 201
ScrFI CCNGG 2 cut(s) 604, 620
SfaNI GCATC 2 cut(s) 329, 642
SfcI CTRYAG 1 cut(s) 628
SgrAI CRCCGGYG 1 cut(s) 641
SinI GGWCC 1 cut(s) 201
SphI GCATGC 1 cut(s) 441
Sse9I AATT 3 cut(s) 82, 235, 390
SsiI CCGC 3 cut(s) 186, 372, 449
StyD4I CCNGG 2 cut(s) 602, 618
StyI CCWWGG 1 cut(s) 216
TaaI ACNGT 1 cut(s) 208
TaqI TCGA 6 cut(s) 86, 92, 156, 239, 337, 379
TasI AATT 3 cut(s) 82, 235, 390
TatI WGTACW 1 cut(s) 480
TauI GCSGC 1 cut(s) 188
TfiI GAWTC 2 cut(s) 257, 614
TscAI CASTG 3 cut(s) 135, 211, 462
TseI GCWGC 2 cut(s) 193, 548
TspDTI ATGAA 2 cut(s) 391, 498
TspRI CASTG 3 cut(s) 135, 211, 462
VpaK11BI GGWCC 1 cut(s) 201
XapI RAATTY 2 cut(s) 82, 235
XceI RCATGY 1 cut(s) 441
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.