pycom05g30070

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0012906
Physical Location & Seq
Reverse (-)
30299707 .. 30300561
855 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g30070.3

Sequence Viewer

Length: 663 bp
ATGGTCCAAACCTATAGTCCATTCGTGCCACATGATCCAGTTAAGCTCAATCTACCTGGCCATCCAAATCCCAAAATCTCCCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTTCAGCCTCCACCACAAATAATAAACCTTGACAACAAAACCGATAAGCTTTTTCTTCTTCTCTCTCGTTTGCCACTGCAACCACATTTACTCTTCGTCGGAAACATGTTCTCAGTGAAGCCTTGTAATAGCCGTTGCAACTCACCATTCCTCTCCGACTCACGTCGTCTTCAAAACCTAGCAAAACAGCAAAGTACCGTTTTCACCCCATGCAGGGTATTGTCTCCTCATGAAAGAAGACTTGGCGTGAGGGCAAGTAGTGGAAAAGGAGAAGAAGAGAAGAAAGAAAGACGACCTTTCTTAACCCTAGAAGAAGCTGGTCTGGTAGAAATCTCCGGTCTCAGCACTCACGAAAGATTTCTATGTCGTTTAACGATATCGTCGTTGAATTTGCTGAGAGTGATATCAGAACAAGAAGGATGCGAAATTGAGGAGCTAAATGCTGGGAAGGTATGTGACTGGTTTGTGAAGGATAAGTTGAAAAGAGAGCAGAACTTGGACTCTGCTGTCCTCCAGTGGGATGATTCTGAGCTATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

221

Amino Acids

24.86

Weight (kDa)

8.22

Isoelectric Point (pI)

63.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012906)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21445
fragaria_vesca FvH4_3g03820 FvH4_3g03870
malus_domestica MD05G1326300.v1.1 MD10G1304300.v1.1
prunus_persica Prupe.4G035400_v2.0.a1
pyrus_communis pycom05g30070 pycom10g25680
rosa_chinensis RchiOBHm_Chr5g0006121
rosa_laevigata RLG00000031381
rosa_multiflora Rmu_sc0004331.1_g000011
rosa_roxburghii Rroxscaffold_1G00069880
rosa_rugosa Rorug04G0419600
rosa_samantha Rh5AG047400 Rh5BG046100 Rh5CG054800 Rh5DG045900
rosa_wichuraiana Rw5G004730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 629
AclWI GGATC 1 cut(s) 29
AcoI YGGCCR 1 cut(s) 58
AcsI RAATTY 1 cut(s) 511
AcuI CTGAAG 1 cut(s) 110
AfaI GTAC 1 cut(s) 319
AfiI CCNNNNNNNGG 3 cut(s) 336, 337, 640
AflIII ACRYGT 1 cut(s) 228
AgsI TTSAA 3 cut(s) 296, 511, 604
AjiI CACGTC 1 cut(s) 287
AjnI CCWGG 1 cut(s) 55
AjuI GAANNNNNNNTTGG 2 cut(s) 348, 380
AluBI AGCT 5 cut(s) 46, 172, 440, 559, 655
AluI AGCT 5 cut(s) 46, 172, 440, 559, 655
Alw26I GTCTC 2 cut(s) 351, 467
AlwI GGATC 1 cut(s) 29
AoxI GGCC 1 cut(s) 58
ApoI RAATTY 1 cut(s) 511
ArsI GACNNNNNNTTYG 1 cut(s) 33
Asp700I GAANNNNTTC 1 cut(s) 480
AspS9I GGNCC 1 cut(s) 4
AsuHPI GGTGA 2 cut(s) 258, 319
AvaII GGWCC 1 cut(s) 4
BalI TGGCCA 1 cut(s) 60
BbsI GAAGAC 2 cut(s) 284, 367
BccI CCATC 1 cut(s) 69
BceAI ACGGC 1 cut(s) 240
BciT130I CCWGG 1 cut(s) 57
BcoDI GTCTC 2 cut(s) 351, 467
BfaI CTAG 2 cut(s) 302, 431
BfmI CTRYAG 1 cut(s) 13
Bme1390I CCNGG 1 cut(s) 57
Bme18I GGWCC 1 cut(s) 4
BmgBI CACGTC 1 cut(s) 287
BmgT120I GGNCC 1 cut(s) 4
BmrFI CCNGG 1 cut(s) 57
BmsI GCATC 1 cut(s) 533
BoxI GACNNNNGTC 1 cut(s) 285
BpiI GAAGAC 2 cut(s) 284, 367
BpmI CTGGAG 1 cut(s) 620
BsaI GGTCTC 1 cut(s) 467
BsaWI WCCGGW 1 cut(s) 458
Bsc4I CCNNNNNNNGG 3 cut(s) 336, 337, 640
Bse1I ACTGG 3 cut(s) 38, 587, 637
BseBI CCWGG 1 cut(s) 57
BseGI GGATG 3 cut(s) 61, 548, 649
BseLI CCNNNNNNNGG 3 cut(s) 336, 337, 640
BseMII CTCAG 4 cut(s) 249, 478, 509, 642
BseNI ACTGG 3 cut(s) 38, 587, 637
BseRI GAGGAG 2 cut(s) 339, 569
BseYI CCCAGC 1 cut(s) 566
BshFI GGCC 1 cut(s) 60
BsiSI CCGG 1 cut(s) 459
BslI CCNNNNNNNGG 3 cut(s) 336, 337, 640
BsmAI GTCTC 2 cut(s) 351, 467
BsnI GGCC 1 cut(s) 60
Bso31I GGTCTC 1 cut(s) 467
Bsp143I GATC 1 cut(s) 34
BspANI GGCC 1 cut(s) 60
BspCNI CTCAG 4 cut(s) 248, 477, 510, 643
BspHI TCATGA 1 cut(s) 352
BspPI GGATC 1 cut(s) 29
BspTNI GGTCTC 1 cut(s) 467
BsrI ACTGG 3 cut(s) 38, 587, 637
BssMI GATC 1 cut(s) 34
Bst2UI CCWGG 1 cut(s) 57
Bst4CI ACNGT 1 cut(s) 322
Bst6I CTCTTC 3 cut(s) 129, 221, 393
BstDEI CTNAG 4 cut(s) 235, 464, 518, 651
BstF5I GGATG 3 cut(s) 61, 548, 649
BstKTI GATC 1 cut(s) 37
BstMAI GTCTC 2 cut(s) 351, 467
BstMBI GATC 1 cut(s) 34
BstNI CCWGG 1 cut(s) 57
BstNSI RCATGY 1 cut(s) 232
BstPAI GACNNNNGTC 1 cut(s) 285
BstSCI CCNGG 1 cut(s) 55
BstSFI CTRYAG 1 cut(s) 13
BstV2I GAAGAC 2 cut(s) 284, 367
BsuRI GGCC 1 cut(s) 60
BtrI CACGTC 1 cut(s) 287
BtsCI GGATG 3 cut(s) 61, 548, 649
BtsI GCAGTG 1 cut(s) 197
BtsIMutI CAGTG 3 cut(s) 197, 243, 644
CciI TCATGA 1 cut(s) 352
Cfr13I GGNCC 1 cut(s) 4
Csp6I GTAC 1 cut(s) 318
CviAII CATG 4 cut(s) 32, 229, 333, 353
CviJI RGCY 9 cut(s) 46, 60, 130, 172, 244, 255, 440, 559, 655
CviKI_1 RGCY 9 cut(s) 46, 60, 130, 172, 244, 255, 440, 559, 655
CviQI GTAC 1 cut(s) 318
DdeI CTNAG 4 cut(s) 235, 464, 518, 651
DpnI GATC 1 cut(s) 36
DpnII GATC 1 cut(s) 34
DrdI GACNNNNNNGTC 1 cut(s) 629
DseDI GACNNNNNNGTC 1 cut(s) 629
EaeI YGGCCR 1 cut(s) 58
Eam1104I CTCTTC 3 cut(s) 129, 221, 393
EarI CTCTTC 3 cut(s) 129, 221, 393
Eco31I GGTCTC 1 cut(s) 467
Eco32I GATATC 2 cut(s) 501, 528
Eco47I GGWCC 1 cut(s) 4
Eco57I CTGAAG 1 cut(s) 110
EcoRII CCWGG 1 cut(s) 55
EcoRV GATATC 2 cut(s) 501, 528
FaeI CATG 4 cut(s) 35, 232, 336, 356
FaiI YATR 7 cut(s) 15, 33, 230, 334, 354, 487, 577
FalI AAGNNNNNCTT 2 cut(s) 403, 435
FatI CATG 4 cut(s) 31, 228, 332, 352
FokI GGATG 3 cut(s) 48, 555, 656
FspBI CTAG 2 cut(s) 302, 431
GsaI CCCAGC 1 cut(s) 570
GsuI CTGGAG 1 cut(s) 620
HaeIII GGCC 1 cut(s) 60
HapII CCGG 1 cut(s) 459
Hin1II CATG 4 cut(s) 35, 232, 336, 356
HindIII AAGCTT 1 cut(s) 170
HinfI GANTC 3 cut(s) 281, 623, 647
HpaII CCGG 1 cut(s) 459
HphI GGTGA 2 cut(s) 258, 319
Hpy188I TCNGA 4 cut(s) 224, 280, 532, 652
Hpy188III TCNNGA 2 cut(s) 353, 473
Hpy99I CGWCG 3 cut(s) 224, 291, 508
HpyAV CCTTC 3 cut(s) 533, 565, 586
HpyCH4III ACNGT 1 cut(s) 322
HpyCH4IV ACGT 1 cut(s) 286
HpyCH4V TGCA 3 cut(s) 202, 261, 336
HpyF3I CTNAG 4 cut(s) 235, 464, 518, 651
HpySE526I ACGT 1 cut(s) 286
Hsp92II CATG 4 cut(s) 35, 232, 336, 356
Kzo9I GATC 1 cut(s) 34
LmnI GCTCC 1 cut(s) 556
LweI GCATC 1 cut(s) 533
MaeI CTAG 2 cut(s) 302, 431
MaeII ACGT 1 cut(s) 286
MaeIII GTNAC 1 cut(s) 578
MalI GATC 1 cut(s) 36
MboI GATC 1 cut(s) 34
MlsI TGGCCA 1 cut(s) 60
MluCI AATT 2 cut(s) 511, 549
MluNI TGGCCA 1 cut(s) 60
MlyI GAGTC 2 cut(s) 275, 617
MmeI TCCRAC 2 cut(s) 202, 303
MnlI CCTC 7 cut(s) 92, 141, 284, 360, 366, 547, 644
Mox20I TGGCCA 1 cut(s) 60
MroXI GAANNNNTTC 1 cut(s) 480
MscI TGGCCA 1 cut(s) 60
MseI TTAA 4 cut(s) 42, 425, 494, 661
Msp20I TGGCCA 1 cut(s) 60
MspI CCGG 1 cut(s) 459
MspR9I CCNGG 1 cut(s) 57
MvaI CCWGG 1 cut(s) 57
NdeII GATC 1 cut(s) 34
NlaIII CATG 4 cut(s) 35, 232, 336, 356
NmuCI GTSAC 1 cut(s) 578
NspI RCATGY 1 cut(s) 232
PagI TCATGA 1 cut(s) 352
PciI ACATGT 1 cut(s) 228
PcsI WCGNNNNNNNCGW 1 cut(s) 503
PdmI GAANNNNTTC 1 cut(s) 480
PfeI GAWTC 1 cut(s) 647
PleI GAGTC 2 cut(s) 275, 617
PpsI GAGTC 2 cut(s) 275, 617
PscI ACATGT 1 cut(s) 228
PshAI GACNNNNGTC 1 cut(s) 285
Psp6I CCWGG 1 cut(s) 55
PspFI CCCAGC 1 cut(s) 566
PspGI CCWGG 1 cut(s) 55
PspPI GGNCC 1 cut(s) 4
RsaI GTAC 1 cut(s) 319
RsaNI GTAC 1 cut(s) 318
SaqAI TTAA 4 cut(s) 42, 425, 494, 661
Sau3AI GATC 1 cut(s) 34
Sau96I GGNCC 1 cut(s) 4
SchI GAGTC 2 cut(s) 275, 617
ScrFI CCNGG 1 cut(s) 57
SfaNI GCATC 1 cut(s) 533
SfcI CTRYAG 1 cut(s) 13
SinI GGWCC 1 cut(s) 4
Sse9I AATT 2 cut(s) 511, 549
SspMI CTAG 2 cut(s) 302, 431
StyD4I CCNGG 1 cut(s) 55
TaaI ACNGT 1 cut(s) 322
TaiI ACGT 1 cut(s) 289
TasI AATT 2 cut(s) 511, 549
TfiI GAWTC 1 cut(s) 647
Tru1I TTAA 4 cut(s) 42, 425, 494, 661
Tru9I TTAA 4 cut(s) 42, 425, 494, 661
TscAI CASTG 3 cut(s) 204, 243, 644
TseFI GTSAC 1 cut(s) 578
Tsp45I GTSAC 1 cut(s) 578
TspDTI ATGAA 1 cut(s) 369
TspRI CASTG 3 cut(s) 204, 243, 644
VpaK11BI GGWCC 1 cut(s) 4
XapI RAATTY 1 cut(s) 511
XceI RCATGY 1 cut(s) 232
XmnI GAANNNNTTC 1 cut(s) 480
XspI CTAG 2 cut(s) 302, 431
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.