Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr5

Orthogroup ID

OG0007021

Physical Location & Seq

Genomic Coordinates Reverse (-)

31355642 .. 31358153

Gene Structure 2512 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1821 bp

ATGGACAAAGGATGGGGGCTCACTCTTGATTCTGACTCCTTCGGCTACCTCCTCAACAAGCCGCCGACCGCCGCGGTTAAGCTCGACCACCACAACAACAAGAGAAGCAGTTTTTTTGGTGGGGAAAGGATGTTCCCGGGAATGGAATTCCCCGTCAAACTTGGCCGCCGGGAAGAGCAGGATGCATCACAGCCGTCCAGTGACAACAACCGGATGGTTGTGGATGAGGTGGACTTCTTTTCCGATCGTAAGAACAAGCTCAATACCAATAGTACCGATGATCATCATCAGGATTCGAAATCCAATGGTGCAGTCAGTGTCAAGAAGGAGAATTGGACTGGTTTGGATGTTAATACTGGATTGCAGCTTGTTACTGCGAACACCCGAAGTGATCAGTCGATGGTTGACGATGGGATTTCTTCTGATCATGGGGTTAACAAAACAGCAAAGAATCATGAGCTGGCACAGTTGCAAGTGGAGGTGCAACGTATGAATGCTGAAAACCAGAGGCTGAAAGAAATGCTTGGTCAAGTGACCAACAACTACAGTGCATTGCAGATGCATCTTGCTGCAGTTGTACAACAACAAAATCACACTACTGCTGCTCCAGCTGATCAGATTTCACAATTAGACCGTGATCAGAATGCTGAAGCAAAGTCTGATCAAGAAAAGAAGCAAGGGTTGGTGCCAAGACAATTTCTGAATTTGGGTCCGAGAGCCACAGCCGAGACGAATGATGACCAAGTTTCCAATTGTTCATCGGAAGCAAGAACCCGATCAGCTTCACCACCTCAAAACATCGATGAAGTTGTGAAAAACGATCAGATTGGTCCGTTGGATCCGCCAAACTCCAATAATTTCCGTGACGGGAAAAGAGTTGGCGGGGATGAGAGTCCTGAATCTGAATCACAAGGATGGGTGCCCAACAAGGTCCCCAAGCTCAACAATTCTGCGGCTCCTAAGCCAATTGATCAATCAACCGAGGCAACCATGAGGAAAGCCCGCGTCTCCGTCCGAGCCAGATCAGAAGCCCCTATGATTACTGATGGATGTCAATGGAGAAAGTATGGACAGAAGATGGCAAAGGGAAACCCATGTCCTCGTGCATATTATAGGTGCACAATGGCTGTAGGCTGTCCTGTTCGCAAACAAGTACAACGTTGTGCTGAGGACAGAACCATACTGATCACAACATATGAAGGCAACCACAACCACCCACTGCCACCCGCTGCAATGGCCATGGCATCCACCACCACAGCAGCCGCCAGCATGTTGCTTTCGGGGTCCATGGCAAGCGCGGACGGGATGAATATGAACCCTAATAATTTGCTAGCCAGAGCAATTCTTCCTTGCTCATCGAGTGTTGCCACAATTTCAGCCTCAGCTCCATTCCCAACTGTCACATTGGACCTCACCACATCTCCATTACAATTCCAAAGACCAAACACCCCATTTCAAGTCCCTAATTTCCCAGGGCAACCCCAGAGTTTTGGGGCAGCTTCACAACAACTGCCTCAAGGTTTTGGGCAGGCCCTCTACAACCAGTCTAAGTTTTCAGGACTTCAATTGTCCCAGGATTTAATGGGGTCTACTAGTTCTCAACAACAACAACAAATGCAGCAAACTCAGTCTTCCTCGTTTGCTGACACAGTGAGTGCCATCGCGGCTGATCCGAGCTTTACTGCTGCTCTCGCGGCCGCTATCACCTCCATCATTGGTGGAGGAGCTCATCCGAGCAACAACAACAGCGCCTCTACCACGTCCAACAACAGCAGCGGAGCCAACAACAACAAGAACATTAGCAGCTTCCCGGGAAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0006355 GO:0006950 GO:0007154 GO:0008150 GO:0009267 GO:0009605 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0009991 GO:0010033 GO:0010036 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016036 GO:0019219 GO:0019222 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031667 GO:0031668 GO:0031669 GO:0033554 GO:0042221 GO:0042493 GO:0042594 GO:0044212 GO:0045892 GO:0045934 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0071496 GO:0080029 GO:0080090 GO:0080169 GO:0097159 GO:0140110 GO:1901363 GO:1901698 GO:1901700 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WRKY

Protein Analysis

607

Amino Acids

65.42

Weight (kDa)

6.61

Isoelectric Point (pI)

47.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WRKY	PF03106	349 - 406	2.6e-25	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007021)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G62300 AT4G04450 AT4G04450 AT4G22070 AT4G22070 AT4G22070 AT4G22070
fragaria_vesca	FvH4_3g01700 FvH4_3g01700
malus_domestica	MD05G1349800.v1.1 MD10G1324500.v1.1
prunus_persica	Prupe.4G017600_v2.0.a1 Prupe.4G017600_v2.0.a1
pyrus_communis	pycom05g31740 pycom10g27490
rosa_chinensis	RchiOBHm_Chr5g0002561
rosa_laevigata	RLG00000031039
rosa_multiflora	Rmu_sc0010731.1_g000002
rosa_roxburghii	Rroxscaffold_1G00073350
rosa_rugosa	Rorug04G0396900
rosa_samantha	Rh5AG020300 Rh5BG023000 Rh5CG022300 Rh5DG021400
rosa_wichuraiana	Rw5G001870

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	685, 919
AccI	GTMKAC	1 cut(s)	1589
AccII	CGCG	5 cut(s)	74, 1005, 1298, 1664, 1694
AclI	AACGTT	1 cut(s)	1159
AclWI	GGATC	3 cut(s)	833, 846, 1664
AcoI	YGGCCR	3 cut(s)	163, 1236, 1695
AcsI	RAATTY	2 cut(s)	146, 703
AcuI	CTGAAG	1 cut(s)	669
AfaI	GTAC	3 cut(s)	274, 579, 1155
AfiI	CCNNNNNNNGG	1 cut(s)	142
AgsI	TTSAA	2 cut(s)	1457, 1565
AhlI	ACTAGT	1 cut(s)	1592
AjiI	CACGTC	1 cut(s)	1761
AjnI	CCWGG	2 cut(s)	1471, 1572
AjuI	GAANNNNNNNTTGG	2 cut(s)	665, 697
AloI	GAACNNNNNNTCC	2 cut(s)	116, 148
Alw21I	GWGCWC	2 cut(s)	1121, 1729
Alw26I	GTCTC	2 cut(s)	722, 1012
Alw44I	GTGCAC	1 cut(s)	1117
AlwI	GGATC	3 cut(s)	833, 846, 1664
AlwNI	CAGNNNCTG	1 cut(s)	511
Ama87I	CYCGRG	2 cut(s)	136, 1810
AoxI	GGCC	4 cut(s)	163, 1236, 1530, 1695
ApaLI	GTGCAC	1 cut(s)	1117
ApoI	RAATTY	2 cut(s)	146, 703
AspLEI	GCGC	2 cut(s)	1298, 1751
AspS9I	GGNCC	6 cut(s)	710, 830, 931, 1284, 1408, 1531
AsuC2I	CCSGG	5 cut(s)	137, 138, 170, 1811, 1812
AsuHPI	GGTGA	3 cut(s)	777, 1405, 1696
AsuII	TTCGAA	1 cut(s)	296
AsuNHI	GCTAGC	1 cut(s)	1330
AvaI	CYCGRG	2 cut(s)	136, 1810
AvaII	GGWCC	5 cut(s)	710, 830, 931, 1284, 1408
BaeGI	GKGCMC	2 cut(s)	924, 1121
BalI	TGGCCA	1 cut(s)	1238
BamHI	GGATCC	1 cut(s)	838
BanI	GGYRCC	2 cut(s)	685, 919
BanII	GRGCYC	2 cut(s)	21, 1729
BauI	CACGAG	1 cut(s)	1101
BbsI	GAAGAC	1 cut(s)	1623
Bbv12I	GWGCWC	2 cut(s)	1121, 1729
BbvCI	CCTCAGC	2 cut(s)	1167, 1381
BccI	CCATC	9 cut(s)	6, 208, 394, 404, 909, 1040, 1072, 1667, 1718
BceAI	ACGGC	1 cut(s)	178
BciT130I	CCWGG	2 cut(s)	1473, 1574
BclI	TGATCA	8 cut(s)	280, 391, 424, 613, 637, 661, 970, 1185
BcnI	CCSGG	5 cut(s)	137, 138, 170, 1811, 1812
BcoDI	GTCTC	2 cut(s)	722, 1012
BcuI	ACTAGT	1 cut(s)	1592
BfaI	CTAG	2 cut(s)	1331, 1593
BfmI	CTRYAG	3 cut(s)	544, 570, 1128
BfoI	RGCGCY	1 cut(s)	1752
BglI	GCCNNNNNGGC	1 cut(s)	1664
Bme1390I	CCNGG	7 cut(s)	137, 138, 170, 1473, 1574, 1811, 1812
Bme18I	GGWCC	5 cut(s)	710, 830, 931, 1284, 1408
BmeT110I	CYCGRG	2 cut(s)	136, 1810
BmgBI	CACGTC	1 cut(s)	1761
BmgT120I	GGNCC	6 cut(s)	710, 830, 931, 1284, 1408, 1531
BmiI	GGNNCC	8 cut(s)	687, 711, 840, 921, 933, 957, 1285, 1780
BmrFI	CCNGG	7 cut(s)	137, 138, 170, 1473, 1574, 1811, 1812
BmsI	GCATC	5 cut(s)	172, 194, 549, 571, 1253
BmtI	GCTAGC	1 cut(s)	1334
BpiI	GAAGAC	1 cut(s)	1623
BpmI	CTGGAG	1 cut(s)	591
Bpu10I	CCTNAGC	3 cut(s)	960, 1167, 1381
Bpu14I	TTCGAA	1 cut(s)	296
BpuEI	CTTGAG	1 cut(s)	1500
BpuMI	CCSGG	5 cut(s)	137, 138, 170, 1811, 1812
Bsa29I	ATCGAT	1 cut(s)	801
BsaBI	GATNNNNATC	2 cut(s)	282, 285
BsaJI	CCNNGG	9 cut(s)	72, 136, 981, 1239, 1287, 1471, 1472, 1572, 1810
BsaWI	WCCGGW	1 cut(s)	210
BsaXI	ACNNNNNCTCC	4 cut(s)	589, 619, 1405, 1435
Bsc4I	CCNNNNNNNGG	1 cut(s)	142
Bse1I	ACTGG	4 cut(s)	198, 343, 361, 1543
Bse3DI	GCAATG	2 cut(s)	551, 1239
Bse8I	GATNNNNATC	2 cut(s)	282, 285
BseBI	CCWGG	2 cut(s)	1473, 1574
BseCI	ATCGAT	1 cut(s)	801
BseDI	CCNNGG	9 cut(s)	72, 136, 981, 1239, 1287, 1471, 1472, 1572, 1810
BseJI	GATNNNNATC	2 cut(s)	282, 285
BseLI	CCNNNNNNNGG	1 cut(s)	142
BseMI	GCAATG	2 cut(s)	551, 1239
BseMII	CTCAG	3 cut(s)	1158, 1395, 1640
BseNI	ACTGG	4 cut(s)	198, 343, 361, 1543
BseRI	GAGGAG	2 cut(s)	41, 1737
BseSI	GKGCMC	2 cut(s)	924, 1121
BseX3I	CGGCCG	1 cut(s)	1695
BsgI	GTGCAG	1 cut(s)	330
Bsh1236I	CGCG	5 cut(s)	74, 1005, 1298, 1664, 1694
Bsh1285I	CGRYCG	3 cut(s)	69, 247, 1698
BshFI	GGCC	4 cut(s)	165, 1238, 1532, 1697
BshNI	GGYRCC	2 cut(s)	685, 919
BshVI	ATCGAT	1 cut(s)	801
BsiEI	CGRYCG	3 cut(s)	69, 247, 1698
BsiHKAI	GWGCWC	2 cut(s)	1121, 1729
BsiHKCI	CYCGRG	2 cut(s)	136, 1810
BsiSI	CCGG	4 cut(s)	137, 169, 211, 1811
BslFI	GGGAC	3 cut(s)	917, 1445, 1555
BslI	CCNNNNNNNGG	1 cut(s)	142
BsmAI	GTCTC	2 cut(s)	722, 1012
BsmBI	CGTCTC	2 cut(s)	722, 1012
BsmFI	GGGAC	3 cut(s)	917, 1445, 1555
BsmI	GAATGC	2 cut(s)	499, 649
BsnI	GGCC	4 cut(s)	165, 1238, 1532, 1697
BsoBI	CYCGRG	2 cut(s)	136, 1810
Bsp119I	TTCGAA	1 cut(s)	296
Bsp1286I	GDGCHC	4 cut(s)	21, 924, 1121, 1729
Bsp1407I	TGTACA	1 cut(s)	577
Bsp19I	CCATGG	2 cut(s)	1239, 1287
BspANI	GGCC	4 cut(s)	165, 1238, 1532, 1697
BspCNI	CTCAG	3 cut(s)	1159, 1394, 1639
BspDI	ATCGAT	1 cut(s)	801
BspFNI	CGCG	5 cut(s)	74, 1005, 1298, 1664, 1694
BspHI	TCATGA	1 cut(s)	454
BspLI	GGNNCC	8 cut(s)	687, 711, 840, 921, 933, 957, 1285, 1780
BspMAI	CTGCAG	1 cut(s)	574
BspOI	GCTAGC	1 cut(s)	1334
BspPI	GGATC	3 cut(s)	833, 846, 1664
BspQI	GCTCTTC	1 cut(s)	168
BspT104I	TTCGAA	1 cut(s)	296
BspT107I	GGYRCC	2 cut(s)	685, 919
BsrDI	GCAATG	2 cut(s)	551, 1239
BsrGI	TGTACA	1 cut(s)	577
BsrI	ACTGG	4 cut(s)	198, 343, 361, 1543
BssECI	CCNNGG	9 cut(s)	72, 136, 981, 1239, 1287, 1471, 1472, 1572, 1810
BssSI	CACGAG	1 cut(s)	1101
BssT1I	CCWWGG	2 cut(s)	1239, 1287
Bst2BI	CACGAG	1 cut(s)	1101
Bst2UI	CCWGG	2 cut(s)	1473, 1574
Bst4CI	ACNGT	5 cut(s)	468, 548, 635, 1399, 1651
Bst6I	CTCTTC	1 cut(s)	168
BstAUI	TGTACA	1 cut(s)	577
BstBI	TTCGAA	1 cut(s)	296
BstC8I	GCNNGC	6 cut(s)	462, 1003, 1267, 1294, 1332, 1530
BstDEI	CTNAG	5 cut(s)	960, 1167, 1381, 1548, 1626
BstDSI	CCRYGG	3 cut(s)	72, 1239, 1287
BstFNI	CGCG	5 cut(s)	74, 1005, 1298, 1664, 1694
BstH2I	RGCGCY	1 cut(s)	1752
BstHHI	GCGC	2 cut(s)	1298, 1751
BstMAI	GTCTC	2 cut(s)	722, 1012
BstMCI	CGRYCG	3 cut(s)	69, 247, 1698
BstMWI	GCNNNNNNNGC	5 cut(s)	608, 1235, 1664, 1691, 1694
BstNI	CCWGG	2 cut(s)	1473, 1574
BstNSI	RCATGY	1 cut(s)	1273
BstSCI	CCNGG	7 cut(s)	135, 136, 168, 1471, 1572, 1809, 1810
BstSFI	CTRYAG	3 cut(s)	544, 570, 1128
BstSLI	GKGCMC	2 cut(s)	924, 1121
BstUI	CGCG	5 cut(s)	74, 1005, 1298, 1664, 1694
BstV2I	GAAGAC	1 cut(s)	1623
BstX2I	RGATCY	1 cut(s)	838
BstXI	CCANNNNNNTGG	1 cut(s)	1490
BstYI	RGATCY	1 cut(s)	838
BstZI	CGGCCG	1 cut(s)	1695
Bsu15I	ATCGAT	1 cut(s)	801
BsuRI	GGCC	4 cut(s)	165, 1238, 1532, 1697
BsuTUI	ATCGAT	1 cut(s)	801
BtgI	CCRYGG	3 cut(s)	72, 1239, 1287
BtgZI	GCGATG	1 cut(s)	1645
BtrI	CACGTC	1 cut(s)	1761
BtsI	GCAGTG	1 cut(s)	1217
BtsIMutI	CAGTG	5 cut(s)	205, 322, 553, 1217, 1656
Cac8I	GCNNGC	6 cut(s)	462, 1003, 1267, 1294, 1332, 1530
CaiI	CAGNNNCTG	1 cut(s)	511
CciI	TCATGA	1 cut(s)	454
CciNI	GCGGCCGC	1 cut(s)	1695
CfoI	GCGC	2 cut(s)	1298, 1751
Cfr13I	GGNCC	6 cut(s)	710, 830, 931, 1284, 1408, 1531
Cfr42I	CCGCGG	1 cut(s)	75
Cfr9I	CCCGGG	2 cut(s)	136, 1810
ClaI	ATCGAT	1 cut(s)	801
CseI	GACGC	1 cut(s)	994
Csp6I	GTAC	3 cut(s)	273, 578, 1154
CviAII	CATG	7 cut(s)	428, 455, 991, 1095, 1240, 1270, 1288
CviQI	GTAC	3 cut(s)	273, 578, 1154
DdeI	CTNAG	5 cut(s)	960, 1167, 1381, 1548, 1626
EaeI	YGGCCR	3 cut(s)	163, 1236, 1695
EagI	CGGCCG	1 cut(s)	1695
Eam1104I	CTCTTC	1 cut(s)	168
EarI	CTCTTC	1 cut(s)	168
EciI	GGCGGA	1 cut(s)	831
Ecl136II	GAGCTC	1 cut(s)	1727
EclXI	CGGCCG	1 cut(s)	1695
Eco130I	CCWWGG	2 cut(s)	1239, 1287
Eco24I	GRGCYC	2 cut(s)	21, 1729
Eco47I	GGWCC	5 cut(s)	710, 830, 931, 1284, 1408
Eco52I	CGGCCG	1 cut(s)	1695
Eco53kI	GAGCTC	1 cut(s)	1727
Eco57I	CTGAAG	1 cut(s)	669
Eco88I	CYCGRG	2 cut(s)	136, 1810
EcoICRI	GAGCTC	1 cut(s)	1727
EcoO109I	RGGNCCY	2 cut(s)	931, 1531
EcoRI	GAATTC	1 cut(s)	146
EcoRII	CCWGG	2 cut(s)	1471, 1572
EcoT14I	CCWWGG	2 cut(s)	1239, 1287
EcoT22I	ATGCAT	2 cut(s)	187, 564
EcoT38I	GRGCYC	2 cut(s)	21, 1729
ErhI	CCWWGG	2 cut(s)	1239, 1287
Esp3I	CGTCTC	2 cut(s)	722, 1012
FaeI	CATG	7 cut(s)	431, 458, 994, 1098, 1243, 1273, 1291
FalI	AAGNNNNNCTT	2 cut(s)	507, 539
FaqI	GGGAC	3 cut(s)	917, 1445, 1555
FatI	CATG	7 cut(s)	427, 454, 990, 1094, 1239, 1269, 1287
FauI	CCCGC	3 cut(s)	875, 1010, 1234
FauNDI	CATATG	1 cut(s)	1195
FbaI	TGATCA	8 cut(s)	280, 391, 424, 613, 637, 661, 970, 1185
FblI	GTMKAC	1 cut(s)	1589
FriOI	GRGCYC	2 cut(s)	21, 1729
FspBI	CTAG	2 cut(s)	1331, 1593
GlaI	GCGC	2 cut(s)	1297, 1750
GsuI	CTGGAG	1 cut(s)	591
HaeII	RGCGCY	1 cut(s)	1752
HaeIII	GGCC	4 cut(s)	165, 1238, 1532, 1697
HapII	CCGG	4 cut(s)	137, 169, 211, 1811
HgaI	GACGC	1 cut(s)	994
HhaI	GCGC	2 cut(s)	1298, 1751
Hin1II	CATG	7 cut(s)	431, 458, 994, 1098, 1243, 1273, 1291
Hin6I	GCGC	2 cut(s)	1296, 1749
HinP1I	GCGC	2 cut(s)	1296, 1749
HincII	GTYRAC	2 cut(s)	406, 436
HindII	GTYRAC	2 cut(s)	406, 436
HinfI	GANTC	7 cut(s)	29, 35, 293, 451, 892, 899, 905
HpaI	GTTAAC	1 cut(s)	436
HpaII	CCGG	4 cut(s)	137, 169, 211, 1811
HphI	GGTGA	3 cut(s)	777, 1405, 1696
Hpy166II	GTNNAC	5 cut(s)	232, 406, 436, 1119, 1590
Hpy188III	TCNNGA	7 cut(s)	26, 290, 322, 455, 665, 896, 1557
Hpy8I	GTNNAC	5 cut(s)	232, 406, 436, 1119, 1590
HpyAV	CCTTC	3 cut(s)	49, 319, 1193
HpyCH4III	ACNGT	5 cut(s)	468, 548, 635, 1399, 1651
HpyCH4IV	ACGT	3 cut(s)	487, 1159, 1760
HpyF10VI	GCNNNNNNNGC	5 cut(s)	608, 1235, 1664, 1691, 1694
HpyF3I	CTNAG	5 cut(s)	960, 1167, 1381, 1548, 1626
HpySE526I	ACGT	3 cut(s)	487, 1159, 1760
Hsp92II	CATG	7 cut(s)	431, 458, 994, 1098, 1243, 1273, 1291
HspAI	GCGC	2 cut(s)	1296, 1749
Ksp22I	TGATCA	8 cut(s)	280, 391, 424, 613, 637, 661, 970, 1185
KspAI	GTTAAC	1 cut(s)	436
KspI	CCGCGG	1 cut(s)	75
LguI	GCTCTTC	1 cut(s)	168
LmnI	GCTCC	5 cut(s)	610, 961, 1390, 1724, 1778
LweI	GCATC	5 cut(s)	172, 194, 549, 571, 1253
MaeI	CTAG	2 cut(s)	1331, 1593
MaeII	ACGT	3 cut(s)	487, 1159, 1760
MaeIII	GTNAC	5 cut(s)	200, 370, 532, 863, 1399
MboII	GAAGA	5 cut(s)	185, 411, 1087, 1337, 1623
MfeI	CAATTG	3 cut(s)	751, 966, 1565
MflI	RGATCY	1 cut(s)	838
MhlI	GDGCHC	4 cut(s)	21, 924, 1121, 1729
MlsI	TGGCCA	1 cut(s)	1238
MluNI	TGGCCA	1 cut(s)	1238
MlyI	GAGTC	2 cut(s)	29, 901
MmeI	TCCRAC	2 cut(s)	816, 1788
Mox20I	TGGCCA	1 cut(s)	1238
Mph1103I	ATGCAT	2 cut(s)	187, 564
MscI	TGGCCA	1 cut(s)	1238
MseI	TTAA	5 cut(s)	78, 351, 435, 1580, 1819
MslI	CAYNNNNRTG	1 cut(s)	913
Msp20I	TGGCCA	1 cut(s)	1238
MspA1I	CMGCKG	4 cut(s)	74, 611, 1229, 1776
MspI	CCGG	4 cut(s)	137, 169, 211, 1811
MspR9I	CCNGG	7 cut(s)	137, 138, 170, 1473, 1574, 1811, 1812
MunI	CAATTG	3 cut(s)	751, 966, 1565
Mva1269I	GAATGC	2 cut(s)	499, 649
MvaI	CCWGG	2 cut(s)	1473, 1574
MvnI	CGCG	5 cut(s)	74, 1005, 1298, 1664, 1694
MwoI	GCNNNNNNNGC	5 cut(s)	608, 1235, 1664, 1691, 1694
NciI	CCSGG	5 cut(s)	137, 138, 170, 1811, 1812
NcoI	CCATGG	2 cut(s)	1239, 1287
NdeI	CATATG	1 cut(s)	1195
NheI	GCTAGC	1 cut(s)	1330
NlaIII	CATG	7 cut(s)	431, 458, 994, 1098, 1243, 1273, 1291
NlaIV	GGNNCC	8 cut(s)	687, 711, 840, 921, 933, 957, 1285, 1780
NmeAIII	GCCGAG	1 cut(s)	751
NmuCI	GTSAC	4 cut(s)	200, 532, 863, 1399
NotI	GCGGCCGC	1 cut(s)	1695
NsiI	ATGCAT	2 cut(s)	187, 564
NspI	RCATGY	1 cut(s)	1273
NspV	TTCGAA	1 cut(s)	296
PagI	TCATGA	1 cut(s)	454
PasI	CCCWGGG	1 cut(s)	1472
PciSI	GCTCTTC	1 cut(s)	168
PctI	GAATGC	2 cut(s)	499, 649
PfeI	GAWTC	5 cut(s)	29, 293, 451, 899, 905
Ple19I	CGATCG	1 cut(s)	247
PleI	GAGTC	2 cut(s)	29, 900
PpsI	GAGTC	2 cut(s)	29, 900
PpuMI	RGGWCCY	1 cut(s)	931
Psp124BI	GAGCTC	1 cut(s)	1729
Psp1406I	AACGTT	1 cut(s)	1159
Psp5II	RGGWCCY	1 cut(s)	931
Psp6I	CCWGG	2 cut(s)	1471, 1572
PspGI	CCWGG	2 cut(s)	1471, 1572
PspN4I	GGNNCC	8 cut(s)	687, 711, 840, 921, 933, 957, 1285, 1780
PspPI	GGNCC	6 cut(s)	710, 830, 931, 1284, 1408, 1531
PspPPI	RGGWCCY	1 cut(s)	931
PstI	CTGCAG	1 cut(s)	574
PstNI	CAGNNNCTG	1 cut(s)	511
PsuI	RGATCY	1 cut(s)	838
PvuI	CGATCG	1 cut(s)	247
PvuII	CAGCTG	1 cut(s)	611
RsaI	GTAC	3 cut(s)	274, 579, 1155
RsaNI	GTAC	3 cut(s)	273, 578, 1154
RseI	CAYNNNNRTG	1 cut(s)	913
SacI	GAGCTC	1 cut(s)	1729
SacII	CCGCGG	1 cut(s)	75
SapI	GCTCTTC	1 cut(s)	168
SaqAI	TTAA	5 cut(s)	78, 351, 435, 1580, 1819
Sau96I	GGNCC	6 cut(s)	710, 830, 931, 1284, 1408, 1531
SchI	GAGTC	2 cut(s)	29, 901
ScrFI	CCNGG	7 cut(s)	137, 138, 170, 1473, 1574, 1811, 1812
SduI	GDGCHC	4 cut(s)	21, 924, 1121, 1729
SfaNI	GCATC	5 cut(s)	172, 194, 549, 571, 1253
SfcI	CTRYAG	3 cut(s)	544, 570, 1128
Sfr303I	CCGCGG	1 cut(s)	75
SfuI	TTCGAA	1 cut(s)	296
SgrBI	CCGCGG	1 cut(s)	75
SinI	GGWCC	5 cut(s)	710, 830, 931, 1284, 1408
SmaI	CCCGGG	2 cut(s)	138, 1812
SmiMI	CAYNNNNRTG	1 cut(s)	913
SmlI	CTYRAG	1 cut(s)	1515
SmoI	CTYRAG	1 cut(s)	1515
SpeI	ACTAGT	1 cut(s)	1592
SspMI	CTAG	2 cut(s)	1331, 1593
SstI	GAGCTC	1 cut(s)	1729
StyD4I	CCNGG	7 cut(s)	135, 136, 168, 1471, 1572, 1809, 1810
StyI	CCWWGG	2 cut(s)	1239, 1287
TaaI	ACNGT	5 cut(s)	468, 548, 635, 1399, 1651
TaiI	ACGT	3 cut(s)	490, 1162, 1763
TaqI	TCGA	5 cut(s)	84, 296, 398, 801, 1358
TatI	WGTACW	2 cut(s)	577, 1153
TauI	GCSGC	8 cut(s)	64, 74, 168, 956, 1265, 1667, 1697, 1700
TfiI	GAWTC	5 cut(s)	29, 293, 451, 899, 905
Tru1I	TTAA	5 cut(s)	78, 351, 435, 1580, 1819
Tru9I	TTAA	5 cut(s)	78, 351, 435, 1580, 1819
TscAI	CASTG	5 cut(s)	205, 322, 553, 1224, 1656
TseFI	GTSAC	4 cut(s)	200, 532, 863, 1399
Tsp45I	GTSAC	4 cut(s)	200, 532, 863, 1399
TspDTI	ATGAA	6 cut(s)	506, 747, 819, 1212, 1322, 1328
TspGWI	ACGGA	3 cut(s)	822, 851, 1000
TspMI	CCCGGG	2 cut(s)	136, 1810
TspRI	CASTG	5 cut(s)	205, 322, 553, 1224, 1656
VneI	GTGCAC	1 cut(s)	1117
VpaK11BI	GGWCC	5 cut(s)	710, 830, 931, 1284, 1408
XapI	RAATTY	2 cut(s)	146, 703
XceI	RCATGY	1 cut(s)	1273
XmaI	CCCGGG	2 cut(s)	136, 1810
XmiI	GTMKAC	1 cut(s)	1589
XspI	CTAG	2 cut(s)	1331, 1593
Zsp2I	ATGCAT	2 cut(s)	187, 564

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.