pycom06g07670

Putative lipopolysaccharide-modifying enzyme.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0008951
Physical Location & Seq
Reverse (-)
12512007 .. 12514373
2367 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1557 bp
ATGTTGCGTGGATATCGTTTTGCCAGACCTTTCAAGTTTCAGGGAACAAGTTTAAAGGGATCTGCACCAGCTGCTGTTGCAACCAATACTATTCTCTTCGTGGGGCTATTCATTGTTGCTTCGGCCATTCTATCTTCCTTTTGGGTTCAAACCTCCAATTTTCAGATTGGAAGTTCTACAAACCAAACCATAGTAGTCTCCAATGAGTTTAAAAAAACCCCTAAACCAGTTGAATTCCCACTCAACTGTTCCATCGGCAACCAAACTCAGACCTGCCCAGCAAACTACCCTACAACCTTTGGCAATACTGATGAACTTGACCCGTCGCCAAACCCTTTGTGCCCGGACTATTTCCGGTTCATCCACCAGGATCTCATGCCATGGAAACGCACAGGAATCACAAGGGACATGGTGGAGAACGCAAAGAAGACAGCACATTTTAGGCTCGTGATTGTGAAGGGCAAGGTTTATGTTGAGAAGTACAAGCAGTCCATACAAACAAGGGATGTTTTTACCATATGGGGCATTTTGCAGCTTCTTAGGAGGTATCCTGGCAGACTACCTGACTTGGAGTTCATGTTTGACTGTGATGACCAGCCGGTCATCCGATCAAGGGACTACCAGGGACTGAACTCGACTCGGGTACCGCCACTGTTCCGGTATTGTGGTGATAGATGGACAAGGGACATTGTGTTCCCTGATTGGTCCTTCTGGGGTTGGAATGAGATAAACATAAAACCATGGGAAGGTTTGTTGAAAGATGTCAAGAAAGGCAATGAAAGGATCAAATGGATGGACAGAGAACCTTATGCATACTGGAAAGGAAACCCCTTTGTTGCTGATACGAGGGAAGACTTACTCAAATGTAATGTCTCAGGGACACAAGACTGGAATGCTCGCCTATTCATCCAGGATTGGATTCTTGAATCTCAACAAGGCTTCAAGCAATCAAATGTAGCAGACCAATGCACACACAGGTACAAGATCGATCTTGAGGGTTATGCATGGTCTGTGAGCGAGAAGTACATTCTAGCCTGTGATTCAGTAACATTGTTGATAAAACCGAAATACTATGATTTCTTCACAAGAGGTCTGCAACCAGTGCACCATTACTGGCCTATAAGGCATGAAAACAAATGTAGATCCATTAAGTTTGCTGTGGATTGGGGCAACAACCACAAACAAAAGGCACAAGCAATTGGAAAAGCAGCAAGCGACTTCATTCAGGAGGAGCTAAAGATGGACTATGTGTATGACTACATGTTTCATTTGTTAAATGAATATGCTAAACTCTTAAGATTTGAGCCACGAATACCCAAAGGCACTACACATTTGTGCTCGGAGAGTATAGCTTGTCCTGCAAATGAGTCGCCGAAGAAGTCTATGACCGAATCATTGGTTAAGAGTCCATCAGCAACAGACCCTTGCACCATGCCTCCTCCCTATGAACCCCGAGCTCTTGCAAAGTTATACAGGAGGAATATTAATTCAATTACACAAGTGCAACAGTGGGAAGATAAGTACTGGGAAAATCTCTCTAAGCAGCAACAGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00514 map00514
Pfam Domains
Protein Families
Glyco_transf_90

Protein Analysis

519

Amino Acids

60.4

Weight (kDa)

9.02

Isoelectric Point (pI)

44.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_90 PF05686 111 - 505 1.8e-190 Glycosyl transferase family 90
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 599
Acc36I ACCTGC 1 cut(s) 281
Acc65I GGTACC 1 cut(s) 643
AccB1I GGYRCC 1 cut(s) 643
AciI CCGC 1 cut(s) 647
AclWI GGATC 4 cut(s) 67, 378, 791, 1137
AcoI YGGCCR 1 cut(s) 123
AcsI RAATTY 1 cut(s) 233
AfaI GTAC 5 cut(s) 482, 645, 980, 1025, 1523
AfiI CCNNNNNNNGG 2 cut(s) 613, 746
AflII CTTAAG 1 cut(s) 1294
AflIII ACRYGT 1 cut(s) 1260
AgsI TTSAA 7 cut(s) 34, 149, 233, 757, 926, 943, 1491
AjnI CCWGG 4 cut(s) 366, 550, 621, 909
AleI CACNNNNGTG 1 cut(s) 1333
AloI GAACNNNNNNTCC 2 cut(s) 677, 709
AluBI AGCT 5 cut(s) 71, 535, 1234, 1352, 1457
AluI AGCT 5 cut(s) 71, 535, 1234, 1352, 1457
Alw21I GWGCWC 3 cut(s) 1107, 1340, 1459
Alw26I GTCTC 2 cut(s) 202, 877
Alw44I GTGCAC 1 cut(s) 1103
AlwI GGATC 4 cut(s) 67, 378, 791, 1137
AlwNI CAGNNNCTG 2 cut(s) 74, 628
Ama87I CYCGRG 2 cut(s) 639, 1452
AoxI GGCC 2 cut(s) 123, 1115
ApaLI GTGCAC 1 cut(s) 1103
ApeKI GCWGC 4 cut(s) 71, 532, 1208, 1543
ApoI RAATTY 1 cut(s) 233
ArsI GACNNNNNNTTYG 2 cut(s) 855, 887
AseI ATTAAT 1 cut(s) 1485
Asp718I GGTACC 1 cut(s) 643
AspS9I GGNCC 1 cut(s) 705
AsuC2I CCSGG 1 cut(s) 344
AsuHPI GGTGA 1 cut(s) 680
AvaI CYCGRG 2 cut(s) 639, 1452
AvaII GGWCC 1 cut(s) 705
BaeGI GKGCMC 2 cut(s) 344, 1107
BaeI ACNNNNGTAYC 2 cut(s) 627, 660
BanI GGYRCC 1 cut(s) 643
BanII GRGCYC 1 cut(s) 1459
BauI CACGAG 1 cut(s) 446
BbsI GAAGAC 2 cut(s) 434, 858
Bbv12I GWGCWC 3 cut(s) 1107, 1340, 1459
BbvI GCAGC 3 cut(s) 58, 544, 1220
BccI CCATC 5 cut(s) 260, 669, 787, 1234, 1417
BcgI CGANNNNNNTGC 2 cut(s) 1350, 1384
BciT130I CCWGG 4 cut(s) 368, 552, 623, 911
BciVI GTATCC 1 cut(s) 558
BcnI CCSGG 1 cut(s) 344
BcoDI GTCTC 2 cut(s) 202, 877
BfaI CTAG 1 cut(s) 1031
BfrI CTTAAG 1 cut(s) 1294
BfuAI ACCTGC 1 cut(s) 281
BfuI GTATCC 1 cut(s) 558
BglI GCCNNNNNGGC 1 cut(s) 1123
BisI GCNGC 4 cut(s) 72, 533, 1209, 1544
BlsI GCNGC 4 cut(s) 73, 534, 1210, 1545
BmcAI AGTACT 1 cut(s) 1523
Bme1390I CCNGG 5 cut(s) 344, 368, 552, 623, 911
Bme18I GGWCC 1 cut(s) 705
BmeT110I CYCGRG 2 cut(s) 639, 1452
BmgT120I GGNCC 1 cut(s) 705
BmiI GGNNCC 1 cut(s) 645
BmrFI CCNGG 5 cut(s) 344, 368, 552, 623, 911
BmrI ACTGGG 1 cut(s) 1534
BmuI ACTGGG 1 cut(s) 1534
BpiI GAAGAC 2 cut(s) 434, 858
BpuEI CTTGAG 1 cut(s) 1013
BpuMI CCSGG 1 cut(s) 344
Bsa29I ATCGAT 1 cut(s) 987
BsaJI CCNNGG 3 cut(s) 380, 622, 740
BsaWI WCCGGW 2 cut(s) 354, 657
BsaXI ACNNNNNCTCC 4 cut(s) 563, 593, 1420, 1450
Bsc4I CCNNNNNNNGG 2 cut(s) 613, 746
Bse118I RCCGGY 1 cut(s) 598
Bse1I ACTGG 6 cut(s) 227, 821, 893, 1100, 1118, 1529
Bse3DI GCAATG 1 cut(s) 781
BseBI CCWGG 4 cut(s) 368, 552, 623, 911
BseCI ATCGAT 1 cut(s) 987
BseDI CCNNGG 3 cut(s) 380, 622, 740
BseGI GGATG 5 cut(s) 360, 511, 603, 798, 906
BseLI CCNNNNNNNGG 2 cut(s) 613, 746
BseMI GCAATG 1 cut(s) 781
BseMII CTCAG 2 cut(s) 281, 888
BseNI ACTGG 6 cut(s) 227, 821, 893, 1100, 1118, 1529
BseRI GAGGAG 2 cut(s) 1244, 1428
BseSI GKGCMC 2 cut(s) 344, 1107
BseXI GCAGC 3 cut(s) 58, 544, 1220
BseYI CCCAGC 1 cut(s) 277
BsgI GTGCAG 1 cut(s) 48
BshFI GGCC 2 cut(s) 125, 1117
BshNI GGYRCC 1 cut(s) 643
BshVI ATCGAT 1 cut(s) 987
BsiHKAI GWGCWC 3 cut(s) 1107, 1340, 1459
BsiHKCI CYCGRG 2 cut(s) 639, 1452
BsiSI CCGG 4 cut(s) 344, 355, 599, 658
BslFI GGGAC 5 cut(s) 419, 629, 639, 698, 892
BslI CCNNNNNNNGG 2 cut(s) 613, 746
BsmAI GTCTC 2 cut(s) 202, 877
BsmFI GGGAC 5 cut(s) 419, 629, 639, 698, 892
BsmI GAATGC 1 cut(s) 898
BsnI GGCC 2 cut(s) 125, 1117
BsoBI CYCGRG 2 cut(s) 639, 1452
Bsp1286I GDGCHC 4 cut(s) 344, 1107, 1340, 1459
Bsp143I GATC 7 cut(s) 59, 370, 608, 783, 984, 988, 1142
Bsp19I CCATGG 2 cut(s) 380, 740
BspACI CCGC 1 cut(s) 647
BspANI GGCC 2 cut(s) 125, 1117
BspCNI CTCAG 2 cut(s) 280, 887
BspDI ATCGAT 1 cut(s) 987
BspLI GGNNCC 1 cut(s) 645
BspMI ACCTGC 1 cut(s) 281
BspPI GGATC 4 cut(s) 67, 378, 791, 1137
BspT107I GGYRCC 1 cut(s) 643
BspTI CTTAAG 1 cut(s) 1294
BsrDI GCAATG 1 cut(s) 781
BsrFI RCCGGY 1 cut(s) 598
BsrI ACTGG 6 cut(s) 227, 821, 893, 1100, 1118, 1529
BssAI RCCGGY 1 cut(s) 598
BssECI CCNNGG 3 cut(s) 380, 622, 740
BssMI GATC 7 cut(s) 59, 370, 608, 783, 984, 988, 1142
BssSI CACGAG 1 cut(s) 446
BssT1I CCWWGG 2 cut(s) 380, 740
Bst2BI CACGAG 1 cut(s) 446
Bst2UI CCWGG 4 cut(s) 368, 552, 623, 911
Bst4CI ACNGT 5 cut(s) 248, 587, 654, 1509, 1551
Bst6I CTCTTC 1 cut(s) 101
BstAFI CTTAAG 1 cut(s) 1294
BstAPI GCANNNNNTGC 2 cut(s) 71, 1102
BstC8I GCNNGC 2 cut(s) 898, 1213
BstDEI CTNAG 4 cut(s) 267, 539, 874, 1539
BstDSI CCRYGG 2 cut(s) 380, 740
BstF5I GGATG 5 cut(s) 360, 511, 603, 798, 906
BstKTI GATC 7 cut(s) 62, 373, 611, 786, 987, 991, 1145
BstMAI GTCTC 2 cut(s) 202, 877
BstMBI GATC 7 cut(s) 59, 370, 608, 783, 984, 988, 1142
BstMWI GCNNNNNNNGC 5 cut(s) 71, 77, 1102, 1123, 1358
BstNI CCWGG 4 cut(s) 368, 552, 623, 911
BstNSI RCATGY 1 cut(s) 1264
BstSCI CCNGG 5 cut(s) 342, 366, 550, 621, 909
BstSLI GKGCMC 2 cut(s) 344, 1107
BstV1I GCAGC 3 cut(s) 58, 544, 1220
BstV2I GAAGAC 2 cut(s) 434, 858
BstX2I RGATCY 3 cut(s) 59, 370, 1142
BstYI RGATCY 3 cut(s) 59, 370, 1142
Bsu15I ATCGAT 1 cut(s) 987
BsuI GTATCC 1 cut(s) 558
BsuRI GGCC 2 cut(s) 125, 1117
BsuTUI ATCGAT 1 cut(s) 987
BtgI CCRYGG 2 cut(s) 380, 740
BtsCI GGATG 5 cut(s) 360, 511, 603, 798, 906
BtsIMutI CAGTG 3 cut(s) 650, 1107, 1514
BveI ACCTGC 1 cut(s) 281
Cac8I GCNNGC 2 cut(s) 898, 1213
CaiI CAGNNNCTG 2 cut(s) 74, 628
Cfr10I RCCGGY 1 cut(s) 598
Cfr13I GGNCC 1 cut(s) 705
ClaI ATCGAT 1 cut(s) 987
Csp6I GTAC 5 cut(s) 481, 644, 979, 1024, 1522
CviAII CATG 9 cut(s) 376, 381, 409, 577, 741, 1005, 1127, 1261, 1432
CviQI GTAC 5 cut(s) 481, 644, 979, 1024, 1522
DdeI CTNAG 4 cut(s) 267, 539, 874, 1539
DpnI GATC 7 cut(s) 61, 372, 610, 785, 986, 990, 1144
DpnII GATC 7 cut(s) 59, 370, 608, 783, 984, 988, 1142
DraI TTTAAA 2 cut(s) 54, 211
DrdI GACNNNNNNGTC 1 cut(s) 599
DseDI GACNNNNNNGTC 1 cut(s) 599
EaeI YGGCCR 1 cut(s) 123
Eam1104I CTCTTC 1 cut(s) 101
EarI CTCTTC 1 cut(s) 101
Ecl136II GAGCTC 1 cut(s) 1457
Eco130I CCWWGG 2 cut(s) 380, 740
Eco24I GRGCYC 1 cut(s) 1459
Eco32I GATATC 1 cut(s) 14
Eco47I GGWCC 1 cut(s) 705
Eco53kI GAGCTC 1 cut(s) 1457
Eco88I CYCGRG 2 cut(s) 639, 1452
EcoICRI GAGCTC 1 cut(s) 1457
EcoRI GAATTC 1 cut(s) 233
EcoRII CCWGG 4 cut(s) 366, 550, 621, 909
EcoRV GATATC 1 cut(s) 14
EcoT14I CCWWGG 2 cut(s) 380, 740
EcoT22I ATGCAT 2 cut(s) 814, 1006
EcoT38I GRGCYC 1 cut(s) 1459
ErhI CCWWGG 2 cut(s) 380, 740
FaeI CATG 9 cut(s) 379, 384, 412, 580, 744, 1008, 1130, 1264, 1435
FaqI GGGAC 5 cut(s) 419, 629, 639, 698, 892
FatI CATG 9 cut(s) 375, 380, 408, 576, 740, 1004, 1126, 1260, 1431
FauNDI CATATG 1 cut(s) 518
Fnu4HI GCNGC 4 cut(s) 72, 533, 1209, 1544
FokI GGATG 5 cut(s) 347, 518, 590, 805, 893
FriOI GRGCYC 1 cut(s) 1459
Fsp4HI GCNGC 4 cut(s) 72, 533, 1209, 1544
FspBI CTAG 1 cut(s) 1031
GluI GCNGC 4 cut(s) 72, 533, 1209, 1544
GsaI CCCAGC 1 cut(s) 281
HaeIII GGCC 2 cut(s) 125, 1117
HapII CCGG 4 cut(s) 344, 355, 599, 658
Hin1II CATG 9 cut(s) 379, 384, 412, 580, 744, 1008, 1130, 1264, 1435
HinfI GANTC 8 cut(s) 396, 637, 919, 926, 1040, 1367, 1391, 1405
HpaII CCGG 4 cut(s) 344, 355, 599, 658
HphI GGTGA 1 cut(s) 680
Hpy166II GTNNAC 1 cut(s) 1105
Hpy188I TCNGA 4 cut(s) 165, 270, 608, 1342
Hpy188III TCNNGA 5 cut(s) 448, 766, 923, 992, 1226
Hpy8I GTNNAC 1 cut(s) 1105
Hpy99I CGWCG 1 cut(s) 328
HpyAV CCTTC 3 cut(s) 451, 718, 740
HpyCH4III ACNGT 5 cut(s) 248, 587, 654, 1509, 1551
HpyF10VI GCNNNNNNNGC 5 cut(s) 71, 77, 1102, 1123, 1358
HpyF3I CTNAG 4 cut(s) 267, 539, 874, 1539
Hsp92II CATG 9 cut(s) 379, 384, 412, 580, 744, 1008, 1130, 1264, 1435
KpnI GGTACC 1 cut(s) 647
Kzo9I GATC 7 cut(s) 59, 370, 608, 783, 984, 988, 1142
LmnI GCTCC 1 cut(s) 1231
Lsp1109I GCAGC 3 cut(s) 58, 544, 1220
MaeI CTAG 1 cut(s) 1031
MaeIII GTNAC 1 cut(s) 1045
MalI GATC 7 cut(s) 61, 372, 610, 785, 986, 990, 1144
MboI GATC 7 cut(s) 59, 370, 608, 783, 984, 988, 1142
MboII GAAGA 7 cut(s) 88, 126, 439, 863, 1072, 1387, 1526
MfeI CAATTG 1 cut(s) 1197
MflI RGATCY 3 cut(s) 59, 370, 1142
MhlI GDGCHC 4 cut(s) 344, 1107, 1340, 1459
MluCI AATT 5 cut(s) 157, 233, 1197, 1486, 1491
MlyI GAGTC 3 cut(s) 631, 1376, 1414
MmeI TCCRAC 1 cut(s) 698
MnlI CCTC 9 cut(s) 163, 537, 840, 988, 1082, 1222, 1446, 1449, 1470
Mph1103I ATGCAT 2 cut(s) 814, 1006
MseI TTAA 7 cut(s) 53, 210, 1149, 1274, 1295, 1401, 1485
MslI CAYNNNNRTG 1 cut(s) 1333
MspA1I CMGCKG 1 cut(s) 71
MspCI CTTAAG 1 cut(s) 1294
MspI CCGG 4 cut(s) 344, 355, 599, 658
MspR9I CCNGG 5 cut(s) 344, 368, 552, 623, 911
MunI CAATTG 1 cut(s) 1197
Mva1269I GAATGC 1 cut(s) 898
MvaI CCWGG 4 cut(s) 368, 552, 623, 911
MwoI GCNNNNNNNGC 5 cut(s) 71, 77, 1102, 1123, 1358
NciI CCSGG 1 cut(s) 344
NcoI CCATGG 2 cut(s) 380, 740
NdeI CATATG 1 cut(s) 518
NdeII GATC 7 cut(s) 59, 370, 608, 783, 984, 988, 1142
NlaIII CATG 9 cut(s) 379, 384, 412, 580, 744, 1008, 1130, 1264, 1435
NlaIV GGNNCC 1 cut(s) 645
NsiI ATGCAT 2 cut(s) 814, 1006
NspI RCATGY 1 cut(s) 1264
OliI CACNNNNGTG 1 cut(s) 1333
PciI ACATGT 1 cut(s) 1260
PctI GAATGC 1 cut(s) 898
PfeI GAWTC 5 cut(s) 396, 919, 926, 1040, 1391
PfoI TCCNGGA 1 cut(s) 909
PkrI GCNGC 4 cut(s) 73, 534, 1210, 1545
PleI GAGTC 3 cut(s) 631, 1375, 1413
PpsI GAGTC 3 cut(s) 631, 1375, 1413
PscI ACATGT 1 cut(s) 1260
PshBI ATTAAT 1 cut(s) 1485
Psp124BI GAGCTC 1 cut(s) 1459
Psp6I CCWGG 4 cut(s) 366, 550, 621, 909
PspFI CCCAGC 1 cut(s) 277
PspGI CCWGG 4 cut(s) 366, 550, 621, 909
PspN4I GGNNCC 1 cut(s) 645
PspPI GGNCC 1 cut(s) 705
PstNI CAGNNNCTG 2 cut(s) 74, 628
PsuI RGATCY 3 cut(s) 59, 370, 1142
PvuII CAGCTG 1 cut(s) 71
RsaI GTAC 5 cut(s) 482, 645, 980, 1025, 1523
RsaNI GTAC 5 cut(s) 481, 644, 979, 1024, 1522
RseI CAYNNNNRTG 1 cut(s) 1333
SacI GAGCTC 1 cut(s) 1459
SaqAI TTAA 7 cut(s) 53, 210, 1149, 1274, 1295, 1401, 1485
SatI GCNGC 4 cut(s) 72, 533, 1209, 1544
Sau3AI GATC 7 cut(s) 59, 370, 608, 783, 984, 988, 1142
Sau96I GGNCC 1 cut(s) 705
ScaI AGTACT 1 cut(s) 1523
SchI GAGTC 3 cut(s) 631, 1376, 1414
ScrFI CCNGG 5 cut(s) 344, 368, 552, 623, 911
SduI GDGCHC 4 cut(s) 344, 1107, 1340, 1459
SinI GGWCC 1 cut(s) 705
SmiMI CAYNNNNRTG 1 cut(s) 1333
SmlI CTYRAG 2 cut(s) 992, 1294
SmoI CTYRAG 2 cut(s) 992, 1294
Sse9I AATT 5 cut(s) 157, 233, 1197, 1486, 1491
SsiI CCGC 1 cut(s) 647
SspI AATATT 1 cut(s) 1483
SspMI CTAG 1 cut(s) 1031
SstI GAGCTC 1 cut(s) 1459
StyD4I CCNGG 5 cut(s) 342, 366, 550, 621, 909
StyI CCWWGG 2 cut(s) 380, 740
TaaI ACNGT 5 cut(s) 248, 587, 654, 1509, 1551
TaqI TCGA 2 cut(s) 635, 987
TaqII GACCGA 1 cut(s) 1403
TasI AATT 5 cut(s) 157, 233, 1197, 1486, 1491
TatI WGTACW 3 cut(s) 480, 1023, 1521
TfiI GAWTC 5 cut(s) 396, 919, 926, 1040, 1391
Tru1I TTAA 7 cut(s) 53, 210, 1149, 1274, 1295, 1401, 1485
Tru9I TTAA 7 cut(s) 53, 210, 1149, 1274, 1295, 1401, 1485
TscAI CASTG 3 cut(s) 657, 1107, 1514
TseI GCWGC 4 cut(s) 71, 532, 1208, 1543
TspRI CASTG 3 cut(s) 657, 1107, 1514
Vha464I CTTAAG 1 cut(s) 1294
VneI GTGCAC 1 cut(s) 1103
VpaK11BI GGWCC 1 cut(s) 705
VspI ATTAAT 1 cut(s) 1485
XapI RAATTY 1 cut(s) 233
XceI RCATGY 1 cut(s) 1264
XspI CTAG 1 cut(s) 1031
ZrmI AGTACT 1 cut(s) 1523
Zsp2I ATGCAT 2 cut(s) 814, 1006
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.