pycom06g10240

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0014251
Physical Location & Seq
Forward (+)
15617688 .. 15619451
1764 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1110 bp
ATGGCGTTCAAGCTTCTTGTTTCTTTGCTCTGCATTCTGATATCCACCGCCGTTTCACAACCGTATTCTCCTCCGTATCCCCAAGAACTCATCAATTCCATCAGCCCGACTCTCTCACCTTCTTCTTCTTCATTCCCTCCGGCCCCAACAGCTTCAACCGAGTCATTTCAGGCGACTTCCCTCGTCCCGGCGCTCTTTGTCATCGGAGACTCCTCCGTCGACTGTGGCACTAACAACTTCCTCGGAACCTTCGCCCGCGCCGACCGCCTCCCCTACGGTCGAGACTTCGATACCCACCAACCCACTGGGAGGTTCTGCAATGGCAGAATCCCTGTCGATTATCTTGCGCTCCGTCTCGGGCTTCCTTTCGTACCAAGTTATCTTGGGCAGAACGGAAGAGTGGAGGAGATGATTCACGGATTGAATTTTGCGTCTGCCGGTGCCGGAATTATATTCACCAGTGGCTCAGAATTGGGTCAACATGTTTCCTTCACTCAGCAGGTTCAGCAGTTCACAGACACCTATCAGCATTTTATAATAAGTTTGGGAGAGCAAGCTGCAATTGATCTCGTCTCAAATTCGGTGCTTTACATTTCGATTGGAATCAACGACTACATACATTACTATTTGCGTAACGTATCCAACGTGCAAAATCTGTATCTGCCCTGGGGTTTCAATCAGTTTTTAGCATCCACATTGACGCAGGAAATTAAGAACTTGTACAACATAAATGTGAAGAAGGTGGTAGTGATGGGGCTGGCACCGATAGGCTGTGCGCCTCACTATTTATTTCAGTACAGAGTGAAGGATGGTCAATGTATTGAGGATATAAACAACATGGTAATGGAGTTCAACTTTGTGATGAGGTATATGATCGAGGAGCTACGTGAAGAGCTTCCCGCTTCCAACATTATCTTCTGCGATGTGTTTGAAGGTTCCATGGATATTATAAAGAACTTTGTTTCAATGAAACTACTGATGCTTGCTGTGGGTTGGGGGAGCACAAGGGTTTTGTCATGTGTTTATCGCCGGATATGGCGTGCAATGATGCTTCTAATCATGTCTGGTGGGATCAGTTCCATCCAACGGATGCGGTCAATGCCATCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

370

Amino Acids

41.17

Weight (kDa)

6.08

Isoelectric Point (pI)

43.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 65 - 311 5.2e-21 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014251)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G10950 AT4G10950
fragaria_vesca FvH4_5g04800
malus_domestica MD06G1109100.v1.1
prunus_persica Prupe.5G122000_v2.0.a1
pyrus_communis pycom06g10240
rosa_chinensis RchiOBHm_Chr7g0194451
rosa_laevigata RLG00000004200
rosa_multiflora Rmu_sc0030308.1_g000001 Rmu_ssc0000261.1_g000027
rosa_roxburghii Rroxscaffold_3G00261350
rosa_rugosa Rorug07G0015700
rosa_samantha Rh7AG143000 Rh7BG143500 Rh7CG147300 Rh7DG144900
rosa_wichuraiana Rw7G012240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 536, 950
AasI GACNNNNNNGTC 1 cut(s) 215
Acc36I ACCTGC 1 cut(s) 490
AccB1I GGYRCC 2 cut(s) 440, 760
AccI GTMKAC 1 cut(s) 219
AccII CGCG 1 cut(s) 258
AciI CCGC 5 cut(s) 48, 256, 265, 900, 1093
AclWI GGATC 1 cut(s) 1079
AcsI RAATTY 2 cut(s) 424, 577
AfaI GTAC 3 cut(s) 372, 722, 797
AfiI CCNNNNNNNGG 3 cut(s) 187, 309, 1086
AflIII ACRYGT 1 cut(s) 481
AgsI TTSAA 7 cut(s) 10, 156, 424, 676, 853, 932, 966
AjnI CCWGG 1 cut(s) 665
AjuI GAANNNNNNNTTGG 2 cut(s) 899, 931
AluBI AGCT 5 cut(s) 13, 152, 557, 883, 895
AluI AGCT 5 cut(s) 13, 152, 557, 883, 895
Alw21I GWGCWC 1 cut(s) 1004
Alw26I GTCTC 4 cut(s) 201, 276, 359, 577
AlwI GGATC 1 cut(s) 1079
Ama87I CYCGRG 1 cut(s) 356
AoxI GGCC 1 cut(s) 141
ApeKI GCWGC 1 cut(s) 557
ApoI RAATTY 2 cut(s) 424, 577
Asp700I GAANNNNTTC 1 cut(s) 894
AspLEI GCGC 4 cut(s) 193, 260, 349, 778
AspS9I GGNCC 1 cut(s) 142
AsuC2I CCSGG 1 cut(s) 188
AsuHPI GGTGA 2 cut(s) 108, 448
AvaI CYCGRG 1 cut(s) 356
BanI GGYRCC 2 cut(s) 440, 760
Bbv12I GWGCWC 1 cut(s) 1004
BbvI GCAGC 1 cut(s) 544
BccI CCATC 4 cut(s) 107, 745, 803, 1088
BceAI ACGGC 1 cut(s) 35
BcgI CGANNNNNNTGC 2 cut(s) 326, 360
BciT130I CCWGG 1 cut(s) 667
BciVI GTATCC 2 cut(s) 87, 649
BcnI CCSGG 1 cut(s) 188
BcoDI GTCTC 4 cut(s) 201, 276, 359, 577
BfaI CTAG 1 cut(s) 1108
BfoI RGCGCY 1 cut(s) 194
BfuAI ACCTGC 1 cut(s) 490
BfuI GTATCC 2 cut(s) 87, 649
BisI GCNGC 1 cut(s) 558
BlsI GCNGC 1 cut(s) 559
Bme1390I CCNGG 2 cut(s) 188, 667
BmeT110I CYCGRG 1 cut(s) 356
BmgT120I GGNCC 1 cut(s) 142
BmiI GGNNCC 5 cut(s) 144, 247, 442, 762, 937
BmrFI CCNGG 2 cut(s) 188, 667
BmrI ACTGGG 1 cut(s) 315
BmsI GCATC 4 cut(s) 698, 969, 1038, 1080
BmuI ACTGGG 1 cut(s) 315
BpuMI CCSGG 1 cut(s) 188
BsaAI YACGTR 1 cut(s) 887
BsaBI GATNNNNATC 1 cut(s) 602
BsaJI CCNNGG 4 cut(s) 241, 665, 666, 939
Bsc4I CCNNNNNNNGG 3 cut(s) 187, 309, 1086
Bse118I RCCGGY 1 cut(s) 437
Bse1I ACTGG 2 cut(s) 310, 459
Bse3DI GCAATG 2 cut(s) 325, 1050
Bse8I GATNNNNATC 1 cut(s) 602
BseBI CCWGG 1 cut(s) 667
BseDI CCNNGG 4 cut(s) 241, 665, 666, 939
BseGI GGATG 5 cut(s) 689, 814, 1080, 1095, 1103
BseJI GATNNNNATC 1 cut(s) 602
BseLI CCNNNNNNNGG 3 cut(s) 187, 309, 1086
BseMI GCAATG 2 cut(s) 325, 1050
BseMII CTCAG 2 cut(s) 480, 509
BseNI ACTGG 2 cut(s) 310, 459
BseRI GAGGAG 4 cut(s) 60, 202, 419, 893
BseXI GCAGC 1 cut(s) 544
Bsh1236I CGCG 1 cut(s) 258
Bsh1285I CGRYCG 2 cut(s) 265, 280
BshFI GGCC 1 cut(s) 143
BshNI GGYRCC 2 cut(s) 440, 760
BsiEI CGRYCG 2 cut(s) 265, 280
BsiHKAI GWGCWC 1 cut(s) 1004
BsiHKCI CYCGRG 1 cut(s) 356
BsiSI CCGG 5 cut(s) 140, 188, 438, 444, 1030
BslFI GGGAC 1 cut(s) 170
BslI CCNNNNNNNGG 3 cut(s) 187, 309, 1086
BsmAI GTCTC 4 cut(s) 201, 276, 359, 577
BsmBI CGTCTC 2 cut(s) 359, 577
BsmFI GGGAC 1 cut(s) 170
BsmI GAATGC 1 cut(s) 33
BsnI GGCC 1 cut(s) 143
BsoBI CYCGRG 1 cut(s) 356
Bsp1286I GDGCHC 1 cut(s) 1004
Bsp1407I TGTACA 1 cut(s) 720
Bsp143I GATC 3 cut(s) 565, 873, 1071
Bsp19I CCATGG 1 cut(s) 939
BspACI CCGC 5 cut(s) 48, 256, 265, 900, 1093
BspANI GGCC 1 cut(s) 143
BspCNI CTCAG 2 cut(s) 479, 508
BspFNI CGCG 1 cut(s) 258
BspLI GGNNCC 5 cut(s) 144, 247, 442, 762, 937
BspMI ACCTGC 1 cut(s) 490
BspPI GGATC 1 cut(s) 1079
BspQI GCTCTTC 1 cut(s) 885
BspT107I GGYRCC 2 cut(s) 440, 760
BsrDI GCAATG 2 cut(s) 325, 1050
BsrFI RCCGGY 1 cut(s) 437
BsrGI TGTACA 1 cut(s) 720
BsrI ACTGG 2 cut(s) 310, 459
BssAI RCCGGY 1 cut(s) 437
BssECI CCNNGG 4 cut(s) 241, 665, 666, 939
BssMI GATC 3 cut(s) 565, 873, 1071
BssT1I CCWWGG 1 cut(s) 939
Bst2UI CCWGG 1 cut(s) 667
Bst4CI ACNGT 3 cut(s) 63, 224, 278
Bst6I CTCTTC 2 cut(s) 391, 885
BstAUI TGTACA 1 cut(s) 720
BstBAI YACGTR 1 cut(s) 887
BstC8I GCNNGC 5 cut(s) 256, 555, 759, 984, 1041
BstDEI CTNAG 2 cut(s) 466, 495
BstDSI CCRYGG 1 cut(s) 939
BstF5I GGATG 5 cut(s) 689, 814, 1080, 1095, 1103
BstFNI CGCG 1 cut(s) 258
BstH2I RGCGCY 1 cut(s) 194
BstHHI GCGC 4 cut(s) 193, 260, 349, 778
BstKTI GATC 3 cut(s) 568, 876, 1074
BstMAI GTCTC 4 cut(s) 201, 276, 359, 577
BstMBI GATC 3 cut(s) 565, 873, 1071
BstMCI CGRYCG 2 cut(s) 265, 280
BstMWI GCNNNNNNNGC 4 cut(s) 149, 264, 505, 1099
BstNI CCWGG 1 cut(s) 667
BstNSI RCATGY 1 cut(s) 485
BstSCI CCNGG 2 cut(s) 186, 665
BstUI CGCG 1 cut(s) 258
BstV1I GCAGC 1 cut(s) 544
BstXI CCANNNNNNTGG 1 cut(s) 305
BsuI GTATCC 2 cut(s) 87, 649
BsuRI GGCC 1 cut(s) 143
BtgI CCRYGG 1 cut(s) 939
BtgZI GCGATG 1 cut(s) 936
BtsCI GGATG 5 cut(s) 689, 814, 1080, 1095, 1103
BtsIMutI CAGTG 2 cut(s) 303, 466
BveI ACCTGC 1 cut(s) 490
Cac8I GCNNGC 5 cut(s) 256, 555, 759, 984, 1041
CfoI GCGC 4 cut(s) 193, 260, 349, 778
Cfr10I RCCGGY 1 cut(s) 437
Cfr13I GGNCC 1 cut(s) 142
CseI GACGC 2 cut(s) 420, 709
Csp6I GTAC 3 cut(s) 371, 721, 796
CviAII CATG 5 cut(s) 482, 838, 940, 1017, 1060
CviQI GTAC 3 cut(s) 371, 721, 796
DdeI CTNAG 2 cut(s) 466, 495
DpnI GATC 3 cut(s) 567, 875, 1073
DpnII GATC 3 cut(s) 565, 873, 1071
DrdI GACNNNNNNGTC 1 cut(s) 215
DseDI GACNNNNNNGTC 1 cut(s) 215
Eam1104I CTCTTC 2 cut(s) 391, 885
EarI CTCTTC 2 cut(s) 391, 885
Eco130I CCWWGG 1 cut(s) 939
Eco32I GATATC 1 cut(s) 42
Eco88I CYCGRG 1 cut(s) 356
EcoRII CCWGG 1 cut(s) 665
EcoRV GATATC 1 cut(s) 42
EcoT14I CCWWGG 1 cut(s) 939
ErhI CCWWGG 1 cut(s) 939
Esp3I CGTCTC 2 cut(s) 359, 577
FaeI CATG 5 cut(s) 485, 841, 943, 1020, 1063
FaqI GGGAC 1 cut(s) 170
FatI CATG 5 cut(s) 481, 837, 939, 1016, 1059
FauI CCCGC 2 cut(s) 263, 907
FblI GTMKAC 1 cut(s) 219
Fnu4HI GCNGC 1 cut(s) 558
FokI GGATG 5 cut(s) 676, 821, 1067, 1090, 1102
Fsp4HI GCNGC 1 cut(s) 558
FspBI CTAG 1 cut(s) 1108
GlaI GCGC 4 cut(s) 192, 259, 348, 777
GluI GCNGC 1 cut(s) 558
HaeII RGCGCY 1 cut(s) 194
HaeIII GGCC 1 cut(s) 143
HapII CCGG 5 cut(s) 140, 188, 438, 444, 1030
HgaI GACGC 2 cut(s) 420, 709
HhaI GCGC 4 cut(s) 193, 260, 349, 778
Hin1II CATG 5 cut(s) 485, 841, 943, 1020, 1063
Hin6I GCGC 4 cut(s) 191, 258, 347, 776
HinP1I GCGC 4 cut(s) 191, 258, 347, 776
HincII GTYRAC 2 cut(s) 220, 479
HindII GTYRAC 2 cut(s) 220, 479
HindIII AAGCTT 1 cut(s) 11
HinfI GANTC 6 cut(s) 109, 161, 209, 327, 412, 603
HpaII CCGG 5 cut(s) 140, 188, 438, 444, 1030
HphI GGTGA 2 cut(s) 108, 448
Hpy166II GTNNAC 3 cut(s) 220, 479, 513
Hpy188I TCNGA 4 cut(s) 39, 206, 245, 469
Hpy188III TCNNGA 1 cut(s) 281
Hpy8I GTNNAC 3 cut(s) 220, 479, 513
Hpy99I CGWCG 1 cut(s) 221
HpyAV CCTTC 6 cut(s) 129, 259, 499, 733, 799, 926
HpyCH4III ACNGT 3 cut(s) 63, 224, 278
HpyCH4IV ACGT 3 cut(s) 636, 645, 886
HpyCH4V TGCA 5 cut(s) 33, 318, 560, 649, 1043
HpyF10VI GCNNNNNNNGC 4 cut(s) 149, 264, 505, 1099
HpyF3I CTNAG 2 cut(s) 466, 495
HpySE526I ACGT 3 cut(s) 636, 645, 886
Hsp92II CATG 5 cut(s) 485, 841, 943, 1020, 1063
HspAI GCGC 4 cut(s) 191, 258, 347, 776
Kzo9I GATC 3 cut(s) 565, 873, 1071
LguI GCTCTTC 1 cut(s) 885
LmnI GCTCC 3 cut(s) 354, 880, 999
Lsp1109I GCAGC 1 cut(s) 544
LweI GCATC 4 cut(s) 698, 969, 1038, 1080
MaeI CTAG 1 cut(s) 1108
MaeII ACGT 3 cut(s) 636, 645, 886
MaeIII GTNAC 1 cut(s) 632
MalI GATC 3 cut(s) 567, 875, 1073
MboI GATC 3 cut(s) 565, 873, 1071
MboII GAAGA 7 cut(s) 114, 117, 120, 408, 748, 902, 907
MfeI CAATTG 1 cut(s) 561
MhlI GDGCHC 1 cut(s) 1004
MluCI AATT 7 cut(s) 94, 424, 447, 470, 561, 577, 708
MlyI GAGTC 3 cut(s) 103, 170, 203
MmeI TCCRAC 3 cut(s) 666, 930, 1108
MroXI GAANNNNTTC 1 cut(s) 894
MseI TTAA 1 cut(s) 711
MslI CAYNNNNRTG 2 cut(s) 731, 842
MspI CCGG 5 cut(s) 140, 188, 438, 444, 1030
MspR9I CCNGG 2 cut(s) 188, 667
MunI CAATTG 1 cut(s) 561
Mva1269I GAATGC 1 cut(s) 33
MvaI CCWGG 1 cut(s) 667
MvnI CGCG 1 cut(s) 258
MwoI GCNNNNNNNGC 4 cut(s) 149, 264, 505, 1099
NciI CCSGG 1 cut(s) 188
NcoI CCATGG 1 cut(s) 939
NdeII GATC 3 cut(s) 565, 873, 1071
NlaIII CATG 5 cut(s) 485, 841, 943, 1020, 1063
NlaIV GGNNCC 5 cut(s) 144, 247, 442, 762, 937
NspI RCATGY 1 cut(s) 485
PasI CCCWGGG 1 cut(s) 666
PciI ACATGT 1 cut(s) 481
PciSI GCTCTTC 1 cut(s) 885
PcsI WCGNNNNNNNCGW 2 cut(s) 258, 642
PctI GAATGC 1 cut(s) 33
PdmI GAANNNNTTC 1 cut(s) 894
PfeI GAWTC 3 cut(s) 327, 412, 603
PkrI GCNGC 1 cut(s) 559
PleI GAGTC 3 cut(s) 103, 169, 203
PpsI GAGTC 3 cut(s) 103, 169, 203
Ppu21I YACGTR 1 cut(s) 887
PscI ACATGT 1 cut(s) 481
PsiI TTATAA 2 cut(s) 536, 950
Psp6I CCWGG 1 cut(s) 665
PspGI CCWGG 1 cut(s) 665
PspN4I GGNNCC 5 cut(s) 144, 247, 442, 762, 937
PspPI GGNCC 1 cut(s) 142
RsaI GTAC 3 cut(s) 372, 722, 797
RsaNI GTAC 3 cut(s) 371, 721, 796
RseI CAYNNNNRTG 2 cut(s) 731, 842
SalI GTCGAC 1 cut(s) 218
SapI GCTCTTC 1 cut(s) 885
SaqAI TTAA 1 cut(s) 711
SatI GCNGC 1 cut(s) 558
Sau3AI GATC 3 cut(s) 565, 873, 1071
Sau96I GGNCC 1 cut(s) 142
SchI GAGTC 3 cut(s) 103, 170, 203
ScrFI CCNGG 2 cut(s) 188, 667
SduI GDGCHC 1 cut(s) 1004
SfaNI GCATC 4 cut(s) 698, 969, 1038, 1080
SmiMI CAYNNNNRTG 2 cut(s) 731, 842
Sse9I AATT 7 cut(s) 94, 424, 447, 470, 561, 577, 708
SsiI CCGC 5 cut(s) 48, 256, 265, 900, 1093
SspMI CTAG 1 cut(s) 1108
StyD4I CCNGG 2 cut(s) 186, 665
StyI CCWWGG 1 cut(s) 939
TaaI ACNGT 3 cut(s) 63, 224, 278
TaiI ACGT 3 cut(s) 639, 648, 889
TaqI TCGA 6 cut(s) 219, 280, 288, 336, 596, 876
TasI AATT 7 cut(s) 94, 424, 447, 470, 561, 577, 708
TatI WGTACW 2 cut(s) 720, 795
TfiI GAWTC 3 cut(s) 327, 412, 603
Tru1I TTAA 1 cut(s) 711
Tru9I TTAA 1 cut(s) 711
TscAI CASTG 2 cut(s) 310, 466
TseI GCWGC 1 cut(s) 557
TspDTI ATGAA 2 cut(s) 120, 983
TspGWI ACGGA 6 cut(s) 63, 205, 341, 408, 432, 1102
TspRI CASTG 2 cut(s) 310, 466
XapI RAATTY 2 cut(s) 424, 577
XceI RCATGY 1 cut(s) 485
XcmI CCANNNNNNNNNTGG 1 cut(s) 302
XmiI GTMKAC 1 cut(s) 219
XmnI GAANNNNTTC 1 cut(s) 894
XspI CTAG 1 cut(s) 1108
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.