pycom06g10430

RING-H2 zinc finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0015493
Physical Location & Seq
Reverse (-)
15754502 .. 15756687
2186 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 804 bp
ATGGCCGGTCTTCCTCAAACTCCAAACAGCACTAGTAATTCTCACCTTTATCCTCAAGCACTCCAACTTAAACTTTACCAGGCCTTCATATTCTCGATTCCTATCCTTTTTTCCATCATCCTTTTTCTCCTCTTCTACTTGTTCTACCTCAAAAGAAGGGCTTCCGCTCTCTTATCATCTTCTCAACCAGTTCTTCCAAGGAGTAATAATTATAATCTTCAAGCTGCGCCTACCACATATCATGTTTCCACTGATTGTCCGGTGGGTTTGAAGGGGGAGCTCAAGGAAAAGCTTCAAACAATTTTATTTGATGAAGAACAAAGGAAAAAGGATTCACAATGTTGTGTTTGCTTGGGGGAATTCGAAATGGAAGAAGAGTTGCTGCAAGTGCGATCATGCAAACACGTGTTTCATGTTGATTGCATACATCACTGGCTGCATAACAACACAACTTGCCCACTTTGCAGGTGCACTGTGATTCCAATCAGTACCAAACTTGATAGTCCTGCAACACCACCTGCAGGTGCTGATGTGTCCGACCCAGTAGTACAACAGCGGCACCATAGCATAATAATATCCCATCAGAGTCCAGGTAGTATTGTATTGTTGGACCAACAAAATCAGCAACAACAGCATCAAACGGGCAATAATGCTATTTCTGGTGTAACAACACCAACTGAAGAAGGTTCATCAAGTGACCAAAGTAGTACGATTTTAAGGGACTCTGGGAGGTTACCGGGGTACTATACTAATCAAGATTTGAGAGAACCAGTCGTTGTCTACATCCAAACTCATGATTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

268

Amino Acids

29.89

Weight (kDa)

6.15

Isoelectric Point (pI)

64.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 113 - 156 3.6e-12 Ring finger domain
zf-RING_11 PF17123 113 - 142 4.9e-08 RING-like zinc finger
zf-C3HC4_2 PF13923 113 - 155 9.8e-07 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 114 - 156 1.2e-07 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015493)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G24015
fragaria_vesca FvH4_5g11670
malus_domestica MD06G1111300.v1.1 MD14G1132500.v1.1
prunus_persica Prupe.5G124100_v2.0.a1
pyrus_communis pycom06g10430
rosa_chinensis RchiOBHm_Chr7g0186121
rosa_laevigata RLG00000004862
rosa_multiflora Rmu_sc0000194.1_g000030
rosa_roxburghii Rroxscaffold_3G00268410
rosa_rugosa Rorug06G0472200
rosa_samantha Rh7AG069400 Rh7BG085400 Rh7CG078700 Rh7DG078800
rosa_wichuraiana Rw7G006550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 213
AarI CACCTGC 3 cut(s) 456, 512, 526
Acc36I ACCTGC 3 cut(s) 456, 512, 526
AccB1I GGYRCC 1 cut(s) 558
AccBSI CCGCTC 1 cut(s) 167
AccI GTMKAC 1 cut(s) 780
AciI CCGC 2 cut(s) 165, 556
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 359
AcuI CTGAAG 1 cut(s) 699
AcvI CACGTG 1 cut(s) 406
AfaI GTAC 4 cut(s) 490, 549, 709, 743
AfiI CCNNNNNNNGG 1 cut(s) 521
AflIII ACRYGT 2 cut(s) 403, 405
AgsI TTSAA 3 cut(s) 221, 271, 296
AhlI ACTAGT 1 cut(s) 32
AjnI CCWGG 2 cut(s) 78, 589
AluBI AGCT 3 cut(s) 224, 280, 292
AluI AGCT 3 cut(s) 224, 280, 292
Alw21I GWGCWC 2 cut(s) 282, 473
Alw44I GTGCAC 1 cut(s) 469
AlwNI CAGNNNCTG 1 cut(s) 527
AoxI GGCC 2 cut(s) 3, 81
ApaLI GTGCAC 1 cut(s) 469
ApeKI GCWGC 3 cut(s) 224, 382, 436
ApoI RAATTY 1 cut(s) 359
Asp700I GAANNNNTTC 2 cut(s) 160, 291
AspLEI GCGC 1 cut(s) 229
AspS9I GGNCC 1 cut(s) 610
AsuC2I CCSGG 1 cut(s) 738
AsuHPI GGTGA 1 cut(s) 35
AsuII TTCGAA 1 cut(s) 363
AvaII GGWCC 1 cut(s) 610
BaeGI GKGCMC 1 cut(s) 473
BanI GGYRCC 1 cut(s) 558
BanII GRGCYC 1 cut(s) 282
BbrPI CACGTG 1 cut(s) 406
BbsI GAAGAC 1 cut(s) 2
Bbv12I GWGCWC 2 cut(s) 282, 473
BbvI GCAGC 3 cut(s) 211, 369, 423
BccI CCATC 2 cut(s) 122, 588
BciT130I CCWGG 2 cut(s) 80, 591
BcnI CCSGG 1 cut(s) 738
BcuI ACTAGT 1 cut(s) 32
BfaI CTAG 1 cut(s) 33
BfmI CTRYAG 1 cut(s) 519
BfuAI ACCTGC 3 cut(s) 456, 512, 526
BisI GCNGC 4 cut(s) 225, 383, 437, 557
BlsI GCNGC 4 cut(s) 226, 384, 438, 558
Bme1390I CCNGG 3 cut(s) 80, 591, 738
Bme18I GGWCC 1 cut(s) 610
BmgT120I GGNCC 1 cut(s) 610
BmiI GGNNCC 1 cut(s) 560
BmrFI CCNGG 3 cut(s) 80, 591, 738
BmrI ACTGGG 1 cut(s) 536
BmsI GCATC 1 cut(s) 643
BmuI ACTGGG 1 cut(s) 536
BpiI GAAGAC 1 cut(s) 2
Bpu14I TTCGAA 1 cut(s) 363
BpuEI CTTGAG 2 cut(s) 39, 266
BpuMI CCSGG 1 cut(s) 738
BsaAI YACGTR 1 cut(s) 406
BsaBI GATNNNNATC 2 cut(s) 101, 482
BsaJI CCNNGG 2 cut(s) 197, 737
BsaWI WCCGGW 1 cut(s) 259
Bsc4I CCNNNNNNNGG 1 cut(s) 521
Bse118I RCCGGY 1 cut(s) 5
Bse1I ACTGG 4 cut(s) 188, 437, 542, 770
Bse8I GATNNNNATC 2 cut(s) 101, 482
BseBI CCWGG 2 cut(s) 80, 591
BseDI CCNNGG 2 cut(s) 197, 737
BseGI GGATG 2 cut(s) 117, 783
BseJI GATNNNNATC 2 cut(s) 101, 482
BseLI CCNNNNNNNGG 1 cut(s) 521
BseNI ACTGG 4 cut(s) 188, 437, 542, 770
BseRI GAGGAG 1 cut(s) 119
BseSI GKGCMC 1 cut(s) 473
BseXI GCAGC 3 cut(s) 211, 369, 423
BshFI GGCC 2 cut(s) 5, 83
BshNI GGYRCC 1 cut(s) 558
BsiHKAI GWGCWC 2 cut(s) 282, 473
BsiSI CCGG 3 cut(s) 6, 260, 737
BslFI GGGAC 1 cut(s) 734
BslI CCNNNNNNNGG 1 cut(s) 521
BsmFI GGGAC 1 cut(s) 734
BsnI GGCC 2 cut(s) 5, 83
Bsp119I TTCGAA 1 cut(s) 363
Bsp1286I GDGCHC 2 cut(s) 282, 473
Bsp143I GATC 1 cut(s) 392
BspACI CCGC 2 cut(s) 165, 556
BspANI GGCC 2 cut(s) 5, 83
BspHI TCATGA 1 cut(s) 793
BspLI GGNNCC 1 cut(s) 560
BspMAI CTGCAG 1 cut(s) 523
BspMI ACCTGC 3 cut(s) 456, 512, 526
BspT104I TTCGAA 1 cut(s) 363
BspT107I GGYRCC 1 cut(s) 558
BsrBI CCGCTC 1 cut(s) 167
BsrFI RCCGGY 1 cut(s) 5
BsrI ACTGG 4 cut(s) 188, 437, 542, 770
BssAI RCCGGY 1 cut(s) 5
BssECI CCNNGG 2 cut(s) 197, 737
BssMI GATC 1 cut(s) 392
BssT1I CCWWGG 1 cut(s) 197
Bst2UI CCWGG 2 cut(s) 80, 591
Bst4CI ACNGT 1 cut(s) 475
Bst6I CTCTTC 2 cut(s) 137, 369
BstBAI YACGTR 1 cut(s) 406
BstBI TTCGAA 1 cut(s) 363
BstEII GGTNACC 1 cut(s) 732
BstF5I GGATG 2 cut(s) 117, 783
BstHHI GCGC 1 cut(s) 229
BstKTI GATC 1 cut(s) 395
BstMBI GATC 1 cut(s) 392
BstMWI GCNNNNNNNGC 3 cut(s) 388, 462, 631
BstNI CCWGG 2 cut(s) 80, 591
BstPI GGTNACC 1 cut(s) 732
BstSCI CCNGG 3 cut(s) 78, 589, 736
BstSFI CTRYAG 1 cut(s) 519
BstSLI GKGCMC 1 cut(s) 473
BstV1I GCAGC 3 cut(s) 211, 369, 423
BstV2I GAAGAC 1 cut(s) 2
BsuRI GGCC 2 cut(s) 5, 83
BtsCI GGATG 2 cut(s) 117, 783
BtsIMutI CAGTG 3 cut(s) 249, 430, 471
BveI ACCTGC 3 cut(s) 456, 512, 526
CaiI CAGNNNCTG 1 cut(s) 527
CciI TCATGA 1 cut(s) 793
CfoI GCGC 1 cut(s) 229
Cfr10I RCCGGY 1 cut(s) 5
Cfr13I GGNCC 1 cut(s) 610
Csp6I GTAC 4 cut(s) 489, 548, 708, 742
CviAII CATG 4 cut(s) 242, 396, 413, 794
CviJI RGCY 7 cut(s) 5, 83, 161, 224, 280, 292, 436
CviKI_1 RGCY 7 cut(s) 5, 83, 161, 224, 280, 292, 436
CviQI GTAC 4 cut(s) 489, 548, 708, 742
DpnI GATC 1 cut(s) 394
DpnII GATC 1 cut(s) 392
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 137, 369
EarI CTCTTC 2 cut(s) 137, 369
Ecl136II GAGCTC 1 cut(s) 280
Eco130I CCWWGG 1 cut(s) 197
Eco147I AGGCCT 1 cut(s) 83
Eco24I GRGCYC 1 cut(s) 282
Eco47I GGWCC 1 cut(s) 610
Eco53kI GAGCTC 1 cut(s) 280
Eco57I CTGAAG 1 cut(s) 699
Eco72I CACGTG 1 cut(s) 406
Eco91I GGTNACC 1 cut(s) 732
EcoICRI GAGCTC 1 cut(s) 280
EcoO65I GGTNACC 1 cut(s) 732
EcoRI GAATTC 1 cut(s) 359
EcoRII CCWGG 2 cut(s) 78, 589
EcoT14I CCWWGG 1 cut(s) 197
EcoT38I GRGCYC 1 cut(s) 282
ErhI CCWWGG 1 cut(s) 197
FaeI CATG 4 cut(s) 245, 399, 416, 797
FalI AAGNNNNNCTT 2 cut(s) 145, 177
FaqI GGGAC 1 cut(s) 734
FatI CATG 4 cut(s) 241, 395, 412, 793
FblI GTMKAC 1 cut(s) 780
Fnu4HI GCNGC 4 cut(s) 225, 383, 437, 557
FokI GGATG 2 cut(s) 104, 770
FriOI GRGCYC 1 cut(s) 282
Fsp4HI GCNGC 4 cut(s) 225, 383, 437, 557
FspBI CTAG 1 cut(s) 33
GlaI GCGC 1 cut(s) 228
GluI GCNGC 4 cut(s) 225, 383, 437, 557
HaeIII GGCC 2 cut(s) 5, 83
HapII CCGG 3 cut(s) 6, 260, 737
HhaI GCGC 1 cut(s) 229
Hin1II CATG 4 cut(s) 245, 399, 416, 797
Hin6I GCGC 1 cut(s) 227
HinP1I GCGC 1 cut(s) 227
HindIII AAGCTT 1 cut(s) 290
HinfI GANTC 6 cut(s) 97, 332, 478, 586, 722, 797
HpaII CCGG 3 cut(s) 6, 260, 737
HphI GGTGA 1 cut(s) 35
Hpy166II GTNNAC 2 cut(s) 471, 781
Hpy188I TCNGA 2 cut(s) 538, 585
Hpy188III TCNNGA 3 cut(s) 94, 755, 794
Hpy8I GTNNAC 2 cut(s) 471, 781
HpyAV CCTTC 4 cut(s) 94, 150, 265, 677
HpyCH4III ACNGT 1 cut(s) 475
HpyCH4IV ACGT 1 cut(s) 405
HpyCH4V TGCA 8 cut(s) 385, 399, 423, 439, 465, 471, 509, 521
HpyF10VI GCNNNNNNNGC 3 cut(s) 388, 462, 631
HpySE526I ACGT 1 cut(s) 405
Hsp92II CATG 4 cut(s) 245, 399, 416, 797
HspAI GCGC 1 cut(s) 227
Kzo9I GATC 1 cut(s) 392
LmnI GCTCC 1 cut(s) 277
Lsp1109I GCAGC 3 cut(s) 211, 369, 423
LweI GCATC 1 cut(s) 643
MaeI CTAG 1 cut(s) 33
MaeII ACGT 1 cut(s) 405
MaeIII GTNAC 3 cut(s) 664, 695, 732
MalI GATC 1 cut(s) 394
MbiI CCGCTC 1 cut(s) 167
MboI GATC 1 cut(s) 392
MboII GAAGA 8 cut(s) 124, 171, 185, 209, 326, 383, 386, 692
MhlI GDGCHC 2 cut(s) 282, 473
MluCI AATT 4 cut(s) 37, 208, 300, 359
MlyI GAGTC 2 cut(s) 595, 716
MmeI TCCRAC 3 cut(s) 88, 561, 588
MnlI CCTC 5 cut(s) 24, 63, 140, 158, 723
MroXI GAANNNNTTC 2 cut(s) 160, 291
MseI TTAA 2 cut(s) 69, 716
MspA1I CMGCKG 1 cut(s) 556
MspI CCGG 3 cut(s) 6, 260, 737
MspR9I CCNGG 3 cut(s) 80, 591, 738
MvaI CCWGG 2 cut(s) 80, 591
MwoI GCNNNNNNNGC 3 cut(s) 388, 462, 631
NciI CCSGG 1 cut(s) 738
NdeII GATC 1 cut(s) 392
NlaIII CATG 4 cut(s) 245, 399, 416, 797
NlaIV GGNNCC 1 cut(s) 560
NmuCI GTSAC 1 cut(s) 695
NspV TTCGAA 1 cut(s) 363
PagI TCATGA 1 cut(s) 793
PaqCI CACCTGC 3 cut(s) 456, 512, 526
PceI AGGCCT 1 cut(s) 83
PdmI GAANNNNTTC 2 cut(s) 160, 291
PfeI GAWTC 4 cut(s) 97, 332, 478, 797
PkrI GCNGC 4 cut(s) 226, 384, 438, 558
PleI GAGTC 2 cut(s) 594, 716
PmaCI CACGTG 1 cut(s) 406
PmlI CACGTG 1 cut(s) 406
PpsI GAGTC 2 cut(s) 594, 716
Ppu21I YACGTR 1 cut(s) 406
PsiI TTATAA 1 cut(s) 213
Psp124BI GAGCTC 1 cut(s) 282
Psp6I CCWGG 2 cut(s) 78, 589
PspCI CACGTG 1 cut(s) 406
PspEI GGTNACC 1 cut(s) 732
PspGI CCWGG 2 cut(s) 78, 589
PspN4I GGNNCC 1 cut(s) 560
PspPI GGNCC 1 cut(s) 610
PstI CTGCAG 1 cut(s) 523
PstNI CAGNNNCTG 1 cut(s) 527
RsaI GTAC 4 cut(s) 490, 549, 709, 743
RsaNI GTAC 4 cut(s) 489, 548, 708, 742
SacI GAGCTC 1 cut(s) 282
SaqAI TTAA 2 cut(s) 69, 716
SatI GCNGC 4 cut(s) 225, 383, 437, 557
Sau3AI GATC 1 cut(s) 392
Sau96I GGNCC 1 cut(s) 610
SbfI CCTGCAGG 1 cut(s) 523
SchI GAGTC 2 cut(s) 595, 716
ScrFI CCNGG 3 cut(s) 80, 591, 738
SdaI CCTGCAGG 1 cut(s) 523
SduI GDGCHC 2 cut(s) 282, 473
SfaNI GCATC 1 cut(s) 643
SfcI CTRYAG 1 cut(s) 519
SfuI TTCGAA 1 cut(s) 363
SinI GGWCC 1 cut(s) 610
SmlI CTYRAG 2 cut(s) 54, 281
SmoI CTYRAG 2 cut(s) 54, 281
SpeI ACTAGT 1 cut(s) 32
Sse8387I CCTGCAGG 1 cut(s) 523
Sse9I AATT 4 cut(s) 37, 208, 300, 359
SseBI AGGCCT 1 cut(s) 83
SsiI CCGC 2 cut(s) 165, 556
SspMI CTAG 1 cut(s) 33
SstI GAGCTC 1 cut(s) 282
StuI AGGCCT 1 cut(s) 83
StyD4I CCNGG 3 cut(s) 78, 589, 736
StyI CCWWGG 1 cut(s) 197
TaaI ACNGT 1 cut(s) 475
TaiI ACGT 1 cut(s) 408
TaqI TCGA 2 cut(s) 95, 363
TasI AATT 4 cut(s) 37, 208, 300, 359
TatI WGTACW 1 cut(s) 547
TauI GCSGC 1 cut(s) 559
TfiI GAWTC 4 cut(s) 97, 332, 478, 797
Tru1I TTAA 2 cut(s) 69, 716
Tru9I TTAA 2 cut(s) 69, 716
TscAI CASTG 3 cut(s) 256, 437, 478
TseFI GTSAC 1 cut(s) 695
TseI GCWGC 3 cut(s) 224, 382, 436
Tsp45I GTSAC 1 cut(s) 695
TspDTI ATGAA 5 cut(s) 76, 327, 401, 678, 789
TspRI CASTG 3 cut(s) 256, 437, 478
VneI GTGCAC 1 cut(s) 469
VpaK11BI GGWCC 1 cut(s) 610
XapI RAATTY 1 cut(s) 359
XmiI GTMKAC 1 cut(s) 780
XmnI GAANNNNTTC 2 cut(s) 160, 291
XspI CTAG 1 cut(s) 33
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.